Carg19376 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg19376
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptiontRNA-dihydrouridine(47) synthase [NAD(P)(+)]
LocationCarg_Chr19: 6984124 .. 6989713 (+)
RNA-Seq ExpressionCarg19376
SyntenyCarg19376
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCAGTTACTCGTGCGCCCTAGCGCAGCCTCCCACTCGCACACTGATGGAAGACTCAGTTTCGGCGGAGCTCCGGGACCCCACGGCGGTTTCCGAGGAGCCGGCTTCTCTGTCGGCCTCTACCGGAGCTCAGTTGACGCCTGAAGAATTGGTGGCCAAGGCCATAGCCCCTGTTAAGCGTGAATATCTTCGTCCTCCGCCGGTGAGGTCCTCCTGTAACAACGTGAAGGATACCGCTTCCACTGGTAAGAATGATGGTGATGATAAGGCTGCTCCGTCTAGCTTGGTGAAGGAGAAGAAGTCCAAACGTCAAATGAAACGAGAACGCCGTCAGGTTCCTAGTCATTCATGTCGCTTTGTGTTTTTGTAGTGAAAGTTAGCCTGCGTCATTTCTAGTTTTGGATTTTGCTTTCCTAAGCATTACTTGAAATCTAGTTGAAGTGCCTGAAACCTTTTTGAATTGAATGGAGCTTGGAATATGACGTTCCTATCTGAAATTGATGGTTAACGGTTGATTAAACACTATAAAACACTTGCAAACGATTGTGATGAGTATCACTGCCTTGAACTCGGTCTTTTTGCTTGTTGTTCGTATATTTTTGTTGATTCCATGAAGACAAATGATTCTAAGTATACGTTATTGTCTCTTTGATAAGTACCAAATTATGAATAGCAGATTTACTCTGTACAGGAACAGAAGTCCACTAGCCACTTATGTCCTGAAATAGCAAAGACAGGAGATGTTAGCTCATGTCGGTATAGTGATAAATGCCGCTTTAGCCATGATATTGAAGCCTTTAAATCTCAGGTAGTGCATTTAAAATCCCTCTTCTAGCCCCCTTTTCTTTATCTAACAAATTTATTTGTGAGTTTACCTTTATTTTGACAAGAGGTTTATGATCACAGAAACCAGCAGATCTCGATGGAGAGTGTCCCTTCAGTGGTGCCGAACAACTTTGCCCCTACGGTTTAGCATGTAGATTTGCAGGGACCCACAAGGAAGGTGTTCCTGCTGGTTCGTCGGTTGCTTTGAAGAAATGCTCCGAAATGAATGGACTGAATAAAGATGTTCAGAAGCTCTTATGGAAGAACAAGATGAAATTTCCAAAAGCTGATTCCAAACTCAAAGAACTTGGGCTCTTGGTATGACCAGAAAGCATACTTTGTATTTTTATCTCTTCTCACGACACACTGTTACTTAAAGCCTGTCCACGTATCACTTTGTTTTGACTAGATAAGGGGTGAAGAGGCTACAGCTTTTAAGGAATAGATTCTAATGCATTTCTTAATCTATTACCCATAATGTTATCTGGTTCCATCGAATAAAAAGACATTGACGTAAGTTTTGGCGTTGTTAGGGGCATGGAAAGTCAAAATCGAGGGTCAAAGAAGAGAAGGAAGATGTTGTATCAAATGATAGTGCTGCAGACAGAACACGCTGTGGCGAAGTGAATAATGACATGGATGCTGACTTAGATATTCCTGTGGAAATTCCAGAAGAAAATGGGCAGACAGAAGGAACATTTTCATCTGATGAATCTCGACCCCTGAAGAAACCGAAATCTATGTCTTCAGAGAACTGTCATTCTGATAAAATTGACAATGGTGGTTTATATTTTATTACTTTATCTTATAGTTTCATCTTTCTCTTGTTATTTATATCTCTCATGTGTGCAACAGGTGCATGTATGATTGATGCAACTATTAGTGAGGGCCATACACCAATTGATTCTGAAGTCGTGAACGACAGTGTACTTCCTGATAGTGATGCAAGCCTTAAATCACATCCCCGCGAAAAAAAACTTATTGATTTTAGAGATAAGCTGTATCTTGCCCCTCTGACCACTGTTGGGAATCTTCCCTTCAGAAGAGTTTGCAAAGTTTTAGGAGCAGATGTGACATGCGGTGAAATGGCAATGTGTACAAATCTTTTGCAGGTTGTGCATCTTACCCTTGGCTCTGCAGAAATTAATATTAATAAGGGCTCCTTAATTGATTGACATCAGCTAGCTATGAAGTTCCAAATTATAGGTTTTGAAAGTTATTTGTTTGTTCTTATACAACAAACAAATAATATTTAGCCATTTAGTAATTATGAAACTATGCAACTACATTTTAATCTAAAACATGGTTTGGCCCTTTTCATTTTGATCTGAAATGTCATTGTTCATTATTTGGCACGATTCTCATTCATTCACTTGTTAATGATGTTTCTATTTCATATTTCTTAAGATGAGTTGAATTGAACATTCTTTTATATTATTTGTTTTGTGTGGTATAGGGTCAAGCTTCAGAATGGGCATTGTTGAGACGACATTCCTCAGAAGATCTATTTGGTGTTCAGATATGCGGTGCATATCCAGACACAGTATCACGTGCTGTAGAACTTATAGACAAAGAATGCGTGATCGACTTCATAGACATAAACATGGGATGCCCAATTGACATTGTTGTCAATAAGGGTGCTGGATCAGCTCTTCTTATGAAGCCAATGCGTATGAAAAGTGTAGTAGAAACCGCTTCCGGAATTGTGGATACACCCATAACAATTAAGGTATTGATTATACTTGGGTACTTATTTCAATGGAACGACCAAAATTAACTGGTGAAGTTGCTTCTAACTGAGGTTATGGGCACTCTCCTTACGTTTCTCTTTTTCTTCCTTTGTGTTATGAGTTTTTTATTGCCCTAAATTTGTTACATGAATTGGTGATGGTCTCGTGGGAGTTTAGGAGGGCAAAGTTAAATGAAATGAGACGAATGAATACTTTTGGAGAATTTAAGAGGCTAAAAGCTCAAAGGTTGTAATTGTTAGACGAACACGTCTCTCCACAATGGTATGATATTATCCACTTTGAGCATAATCTCTAATGGCTTTGCTTTGGGCTTTCCCAAAAGGCCTCATACCAATGGAGAGAATATTTTTTGATTATAAACCCATGATCATTCCCTAAATTAGTCAGTGTGGGACTTTTATCATCCAACAGTAATTAGGTGGATCTAGCATATACTAGTTGTTTTCTTATTTTGCATTCATAAGATTGAATTCTAGGAATTGAGTTGAAGATTAACACAAATGCCCTGGACCCCTTGATTTTTGGAAAGCTGTCTCCTGTATTAGTTTACATGAGGATAGTATAAGGTGGTTTCTGGATAGCTCCCTCGAGGAGGAGCCCCTTCATAGCTCCCTCTAAGTGTTTGAAAGATGAGAGATGTCAAGCAGCTAAATGTTTTCATTTGGTCGCTTGTTCTGGGAGGTAGTAATACAAATAGTTGTTGCTAAAAATTGTGAGTCTTTATTCGTTTAAGAACTACTTGATTTTTGGTTTTTGAAACTTGTGCTTGCTTCTTTAAAATAGTGGTTTTTAGATTTCAAAACTTGGCTTGGTTTTTGAAAATACTCCTAAAATGTAGATAGCAAAACAAAGAACTCAACTAGTGTAGGTAGCGTTTATAAGTTGATTTTCAAAAACCGAATGACCGACCCAAAAAACAAAACTGCAACAGCTCGAGAGCTCGAGATCTGATTTCAATTGTGTATATTAGGGAGCATAATATCGAACCAGCGTGTTCTTTCTTAGGGGGAGGGGGATCTATATATTTGTTATGTTCTTGGTTTCTCAAGTTCTTCATTTTGTACCATTGTGTTTTTCTTGCATTAGGTTCGGACAGGCTACTTCGAAGGGAAAAACCGTATCGATTCACTTATTGCAGACATTGGTAACTGGGGAGCTGGTGCTGTAACCATTCATGGCCGTACTCGCCAGCAACGTTACAGCAAGCTTGCTGACTGGGAATACATCAATCGCTGTTCCTCGTTAGCTCCTGATTCCCTGCAAGTTATAGGAAATGGGGACGTATTTTCATATGCAGATTGGAATCGACATAAAGTTGAATCCCCAAAGTTAGCTACTTGCATGATTGCTAGAGGAGCATTAATTAAGGTTTTTTTGCAGTTTGATGTTCTTTTATTTGATTCTTCAACTCATTGAAGCTTATGTTGCTATAATCTGTTGCCCATTGCAGCCTTGGTTATTTACTGAAATTAAGGAACAGAGAAATTGGGACATCACTTCAGGGGAGCGGCTAAATATCTTGAAGGATTTTGTACATTTTGGACTTGAGCACTGGGGTTCCGACACAAAAGGTATGTCGCTGGTTGCCTGTTGCTTGTCATACTGCTCGTTGCATTTCGTTGTTGCTACTATTGAACACAACTCTCCGTAGGAAACACTTAGCACTATTTATTAAGGGAAAAGAATACAAGACATTCTGTAGAAACTGCAATCGACCATAGACCAATTCTCCTTTTATTTGGGATGGGAGTATTGACTTGACTACACCCAAAAGCCATATGTCAGATCCGAGGCATCCCAATTCGATTGAATGGGATGACAGTTTCTCATTCTGAATCTGTATGATCAAATCACACCTCGCAATATACTAAGCCTTTCCTTTCTCATTTGATCTAATCTTTACTGTCTCCCGATTGTTTCATAGAGACAATCGGGAGATGGGATGACCTATTACATGCTAGAGCTTATCGAAAAGAAACAAGTTCCTTTTTTTAAGATTCAAATAAAAAAAAGCCAATTATCCATTCAATATAATAGTGGTGATTGAATGCTTGAGCTCTCAGATACTTTCATGAGTCCATATATAAAGTATCTTCCTCCTTTCTGTAACTCAAAATGAAATTCGATTACTTTGTGCTTTGTATTTCTTTTGGGATAAAAATCTAGGTAGGGTGGAGGCTTATTTATAGTATAAGCTAGACAATCTTTTTTCTAAAATATTTAAATATCTGTTAAAATATCTAGGTATCTTTATTCCCCGACCACATAAATCATTGTCCTAAAATGAGTATAGAATGGGCTCATACCATTATTGGGCTTGACCCTATATTGGGCTAGTGATGATATTTTAACGGCCACCGGGCTTCTTCAGCTGATTTTTATGCTATCCTTTCTTCCCATTGTTATATCTGCAGGTGTTGAGACAACAAGGCATTTCTTATTGGAATGGCTTAGCTACACATGCAGATATATTCCCGTTGGTCTTCTGGATGTTGTTCCCCAACGTTTGAACTGGCGCCCGCCGTCGTATTTTGGCCGGGACGACCTCGAGACGCTCATGTCCTCCGACTCTGCAGCTGACTGGGTAATACATTTTTACTTTTTAGTTCAAAAAGTGCTCAAACCATTCGACCTCGAATCGTAATCCCTTCCATTACTACAGATTAAGATCTCAGAGATGTTGCTCGGTAAAGTTCCCGATGCCTTCTCGTTCGCTCCCAAACACAAGTCCAACGCCTACGACCGAGCCGAAAATGGCTGATTCGGGTTCATCACTGTAAGAGAAGATCCGATCACTGAAATTCAAATTCGTTCTTCTGGTGCACACATAAACTGAGACACTTTCCATTGCCATTGTCAGTAGAGCTAATTTAGTTATTTTCACGAATTATTTATGTATTTATGTTCCAATTACATGATAAATCAGGTGCTCGACGCAGCTGTAAATTTATTTATTAATTATTATACCATGCTGACTACATATATTTATTTTAATTCCAATATTATTATTATTATT

mRNA sequence

TCAGTTACTCGTGCGCCCTAGCGCAGCCTCCCACTCGCACACTGATGGAAGACTCAGTTTCGGCGGAGCTCCGGGACCCCACGGCGGTTTCCGAGGAGCCGGCTTCTCTGTCGGCCTCTACCGGAGCTCAGTTGACGCCTGAAGAATTGGTGGCCAAGGCCATAGCCCCTGTTAAGCGTGAATATCTTCGTCCTCCGCCGGTGAGGTCCTCCTGTAACAACGTGAAGGATACCGCTTCCACTGGTAAGAATGATGGTGATGATAAGGCTGCTCCGTCTAGCTTGGTGAAGGAGAAGAAGTCCAAACGTCAAATGAAACGAGAACGCCGTCAGGAACAGAAGTCCACTAGCCACTTATGTCCTGAAATAGCAAAGACAGGAGATGTTAGCTCATGTCGGTATAGTGATAAATGCCGCTTTAGCCATGATATTGAAGCCTTTAAATCTCAGAAACCAGCAGATCTCGATGGAGAGTGTCCCTTCAGTGGTGCCGAACAACTTTGCCCCTACGGTTTAGCATGTAGATTTGCAGGGACCCACAAGGAAGGTGTTCCTGCTGGTTCGTCGGTTGCTTTGAAGAAATGCTCCGAAATGAATGGACTGAATAAAGATGTTCAGAAGCTCTTATGGAAGAACAAGATGAAATTTCCAAAAGCTGATTCCAAACTCAAAGAACTTGGGCTCTTGGGGCATGGAAAGTCAAAATCGAGGGTCAAAGAAGAGAAGGAAGATGTTGTATCAAATGATAGTGCTGCAGACAGAACACGCTGTGGCGAAGTGAATAATGACATGGATGCTGACTTAGATATTCCTGTGGAAATTCCAGAAGAAAATGGGCAGACAGAAGGAACATTTTCATCTGATGAATCTCGACCCCTGAAGAAACCGAAATCTATGTCTTCAGAGAACTGTCATTCTGATAAAATTGACAATGGTGCATGTATGATTGATGCAACTATTAGTGAGGGCCATACACCAATTGATTCTGAAGTCGTGAACGACAGTGTACTTCCTGATAGTGATGCAAGCCTTAAATCACATCCCCGCGAAAAAAAACTTATTGATTTTAGAGATAAGCTGTATCTTGCCCCTCTGACCACTGTTGGGAATCTTCCCTTCAGAAGAGTTTGCAAAGTTTTAGGAGCAGATGTGACATGCGGTGAAATGGCAATGTGTACAAATCTTTTGCAGGGTCAAGCTTCAGAATGGGCATTGTTGAGACGACATTCCTCAGAAGATCTATTTGGTGTTCAGATATGCGGTGCATATCCAGACACAGTATCACGTGCTGTAGAACTTATAGACAAAGAATGCGTGATCGACTTCATAGACATAAACATGGGATGCCCAATTGACATTGTTGTCAATAAGGGTGCTGGATCAGCTCTTCTTATGAAGCCAATGCGTATGAAAAGTGTAGTAGAAACCGCTTCCGGAATTGTGGATACACCCATAACAATTAAGGTTCGGACAGGCTACTTCGAAGGGAAAAACCGTATCGATTCACTTATTGCAGACATTGGTAACTGGGGAGCTGGTGCTGTAACCATTCATGGCCGTACTCGCCAGCAACGTTACAGCAAGCTTGCTGACTGGGAATACATCAATCGCTGTTCCTCGTTAGCTCCTGATTCCCTGCAAGTTATAGGAAATGGGGACGTATTTTCATATGCAGATTGGAATCGACATAAAGTTGAATCCCCAAAGTTAGCTACTTGCATGATTGCTAGAGGAGCATTAATTAAGCCTTGGTTATTTACTGAAATTAAGGAACAGAGAAATTGGGACATCACTTCAGGGGAGCGGCTAAATATCTTGAAGGATTTTGTACATTTTGGACTTGAGCACTGGGGTTCCGACACAAAAGGTGTTGAGACAACAAGGCATTTCTTATTGGAATGGCTTAGCTACACATGCAGATATATTCCCGTTGGTCTTCTGGATGTTGTTCCCCAACGTTTGAACTGGCGCCCGCCGTCGTATTTTGGCCGGGACGACCTCGAGACGCTCATGTCCTCCGACTCTGCAGCTGACTGGATTAAGATCTCAGAGATGTTGCTCGGTAAAGTTCCCGATGCCTTCTCGTTCGCTCCCAAACACAAGTCCAACGCCTACGACCGAGCCGAAAATGGCTGATTCGGGTTCATCACTGTAAGAGAAGATCCGATCACTGAAATTCAAATTCGTTCTTCTGGTGCACACATAAACTGAGACACTTTCCATTGCCATTGTCAGTAGAGCTAATTTAGTTATTTTCACGAATTATTTATGTATTTATGTTCCAATTACATGATAAATCAGGTGCTCGACGCAGCTGTAAATTTATTTATTAATTATTATACCATGCTGACTACATATATTTATTTTAATTCCAATATTATTATTATTATT

Coding sequence (CDS)

ATGGAAGACTCAGTTTCGGCGGAGCTCCGGGACCCCACGGCGGTTTCCGAGGAGCCGGCTTCTCTGTCGGCCTCTACCGGAGCTCAGTTGACGCCTGAAGAATTGGTGGCCAAGGCCATAGCCCCTGTTAAGCGTGAATATCTTCGTCCTCCGCCGGTGAGGTCCTCCTGTAACAACGTGAAGGATACCGCTTCCACTGGTAAGAATGATGGTGATGATAAGGCTGCTCCGTCTAGCTTGGTGAAGGAGAAGAAGTCCAAACGTCAAATGAAACGAGAACGCCGTCAGGAACAGAAGTCCACTAGCCACTTATGTCCTGAAATAGCAAAGACAGGAGATGTTAGCTCATGTCGGTATAGTGATAAATGCCGCTTTAGCCATGATATTGAAGCCTTTAAATCTCAGAAACCAGCAGATCTCGATGGAGAGTGTCCCTTCAGTGGTGCCGAACAACTTTGCCCCTACGGTTTAGCATGTAGATTTGCAGGGACCCACAAGGAAGGTGTTCCTGCTGGTTCGTCGGTTGCTTTGAAGAAATGCTCCGAAATGAATGGACTGAATAAAGATGTTCAGAAGCTCTTATGGAAGAACAAGATGAAATTTCCAAAAGCTGATTCCAAACTCAAAGAACTTGGGCTCTTGGGGCATGGAAAGTCAAAATCGAGGGTCAAAGAAGAGAAGGAAGATGTTGTATCAAATGATAGTGCTGCAGACAGAACACGCTGTGGCGAAGTGAATAATGACATGGATGCTGACTTAGATATTCCTGTGGAAATTCCAGAAGAAAATGGGCAGACAGAAGGAACATTTTCATCTGATGAATCTCGACCCCTGAAGAAACCGAAATCTATGTCTTCAGAGAACTGTCATTCTGATAAAATTGACAATGGTGCATGTATGATTGATGCAACTATTAGTGAGGGCCATACACCAATTGATTCTGAAGTCGTGAACGACAGTGTACTTCCTGATAGTGATGCAAGCCTTAAATCACATCCCCGCGAAAAAAAACTTATTGATTTTAGAGATAAGCTGTATCTTGCCCCTCTGACCACTGTTGGGAATCTTCCCTTCAGAAGAGTTTGCAAAGTTTTAGGAGCAGATGTGACATGCGGTGAAATGGCAATGTGTACAAATCTTTTGCAGGGTCAAGCTTCAGAATGGGCATTGTTGAGACGACATTCCTCAGAAGATCTATTTGGTGTTCAGATATGCGGTGCATATCCAGACACAGTATCACGTGCTGTAGAACTTATAGACAAAGAATGCGTGATCGACTTCATAGACATAAACATGGGATGCCCAATTGACATTGTTGTCAATAAGGGTGCTGGATCAGCTCTTCTTATGAAGCCAATGCGTATGAAAAGTGTAGTAGAAACCGCTTCCGGAATTGTGGATACACCCATAACAATTAAGGTTCGGACAGGCTACTTCGAAGGGAAAAACCGTATCGATTCACTTATTGCAGACATTGGTAACTGGGGAGCTGGTGCTGTAACCATTCATGGCCGTACTCGCCAGCAACGTTACAGCAAGCTTGCTGACTGGGAATACATCAATCGCTGTTCCTCGTTAGCTCCTGATTCCCTGCAAGTTATAGGAAATGGGGACGTATTTTCATATGCAGATTGGAATCGACATAAAGTTGAATCCCCAAAGTTAGCTACTTGCATGATTGCTAGAGGAGCATTAATTAAGCCTTGGTTATTTACTGAAATTAAGGAACAGAGAAATTGGGACATCACTTCAGGGGAGCGGCTAAATATCTTGAAGGATTTTGTACATTTTGGACTTGAGCACTGGGGTTCCGACACAAAAGGTGTTGAGACAACAAGGCATTTCTTATTGGAATGGCTTAGCTACACATGCAGATATATTCCCGTTGGTCTTCTGGATGTTGTTCCCCAACGTTTGAACTGGCGCCCGCCGTCGTATTTTGGCCGGGACGACCTCGAGACGCTCATGTCCTCCGACTCTGCAGCTGACTGGATTAAGATCTCAGAGATGTTGCTCGGTAAAGTTCCCGATGCCTTCTCGTTCGCTCCCAAACACAAGTCCAACGCCTACGACCGAGCCGAAAATGGCTGA

Protein sequence

MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRTRCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACMIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG
Homology
BLAST of Carg19376 vs. NCBI nr
Match: KAG6572077.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011741.1 tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 696/696 (100.00%), Postives = 696/696 (100.00%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60

Query: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240

Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
           RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300

Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
           IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360

Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420

Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
           KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480

Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
           GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540

Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
           SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE
Sbjct: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600

Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
           HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660

Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
           AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696

BLAST of Carg19376 vs. NCBI nr
Match: XP_022952746.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita moschata])

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 693/696 (99.57%), Postives = 695/696 (99.86%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPP+RSSCNNV
Sbjct: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPMRSSCNNV 60

Query: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240

Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
           RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300

Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
           IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360

Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420

Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
           KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480

Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
           GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540

Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
           SYADWNRHKVESPKLATC IARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGLE
Sbjct: 541 SYADWNRHKVESPKLATCTIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGLE 600

Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
           HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660

Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
           AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696

BLAST of Carg19376 vs. NCBI nr
Match: XP_022969416.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima])

HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 681/696 (97.84%), Postives = 687/696 (98.71%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60

Query: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KD ASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240

Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
            C EVNNDMDADLDIP+E+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDNGACM
Sbjct: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGACM 300

Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
           IDATI EGHTPI+SEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360

Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420

Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
           KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFE 480

Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
           GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540

Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
           SYA+WNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE
Sbjct: 541 SYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600

Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
           HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660

Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
           AADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696

BLAST of Carg19376 vs. NCBI nr
Match: XP_023554076.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 680/696 (97.70%), Postives = 687/696 (98.71%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELR PTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSS NNV
Sbjct: 1   MEDSVSAELRVPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSGNNV 60

Query: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KD ASTGKNDGD+KAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDAASTGKNDGDNKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV+LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVSLKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240

Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
            C EVNNDMDADLDIPVE+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM
Sbjct: 241 CCSEVNNDMDADLDIPVEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300

Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
           IDATISEGHTPI+SEVVND VLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATISEGHTPIESEVVNDIVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360

Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420

Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
           KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480

Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
           GKNRIDSLIADIGNWGA AVT+HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGASAVTVHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540

Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
           SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGLE
Sbjct: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGLE 600

Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
           HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660

Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
           AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696

BLAST of Carg19376 vs. NCBI nr
Match: XP_004148548.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 hypothetical protein Csa_020245 [Cucumis sativus])

HSP 1 Score: 1271.5 bits (3289), Expect = 0.0e+00
Identity = 624/696 (89.66%), Postives = 655/696 (94.11%), Query Frame = 0

Query: 2   EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61
           EDSV+A+L D TAVSE P S S+  G  LTP++LVAKAIAPVKR++LRPPP+RSS NN+K
Sbjct: 3   EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNLK 62

Query: 62  DTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSD 121
           D  ST KNDGDDKAAPS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDVSSCRYSD
Sbjct: 63  DATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSD 122

Query: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCS 181
           KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LK+CS
Sbjct: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182

Query: 182 EMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDS-AADRT 241
           EMNGLNKD+QKLLWKNKMKFPKADSKLKELGLLGHGKSK RVKE++E+ VSNDS  ADR 
Sbjct: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242

Query: 242 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 301
            C E+NND+DADL+I VE+PEENG+ EGTFSSDESRPLKK KS+SSEN HSD+IDNGACM
Sbjct: 243 CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACM 302

Query: 302 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 361
           ID TI EGH PI+SE +NDSVL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 303 IDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRR 362

Query: 362 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 421
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 363 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 422

Query: 422 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 481
           KECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+E
Sbjct: 423 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYE 482

Query: 482 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 541
           GKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 483 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 542

Query: 542 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 601
           SY DWN HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGLE
Sbjct: 543 SYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLE 602

Query: 602 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 661
           HWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 603 HWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDS 662

Query: 662 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
           AADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 663 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of Carg19376 vs. ExPASy Swiss-Prot
Match: Q9T0J6 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=3702 GN=At4g38890 PE=1 SV=2)

HSP 1 Score: 869.8 bits (2246), Expect = 2.1e-251
Identity = 447/716 (62.43%), Postives = 544/716 (75.98%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQL-----TPEELVAKAIAPVKREYLRPPPVRS 60
           M D ++    D +   +  A+L   +  Q      T EEL+ +++AP+K+E+L PPP   
Sbjct: 1   MSDVIAEHAVDTSVEGDAKAALPVQSTKQFSVYEATSEELIERSMAPIKKEFLCPPP--- 60

Query: 61  SCNNVKDTASTGKNDGDDKAAP-SSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDV 120
                  + S  +ND  D  AP S LV+EKKSKRQ+KRERR++  ST +LCP++A+T DV
Sbjct: 61  ------PSRSVKQNDAADVRAPQSGLVQEKKSKRQLKRERREQ--STINLCPQVARTEDV 120

Query: 121 SSCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSS 180
            SC+Y DKCRF+HDIEAFK+QK  D++G+CPF  +   C YGL+CRF G+H++    G+S
Sbjct: 121 DSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASGMKCAYGLSCRFLGSHRD--ITGNS 180

Query: 181 VALKKCSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSN- 240
              +K SEMN  NK+ Q+LLWKNKM F  AD+KLK LGLLGH K  +  +E   +   N 
Sbjct: 181 DDKEK-SEMNFFNKETQRLLWKNKMTFTNADAKLKSLGLLGHAKKSNAAEEITAEKTQNG 240

Query: 241 -------DSAADRTRCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSS 300
                  + A D     E  ++M  D+DIP           G   ++E RP+KK KS   
Sbjct: 241 MNGTQATEVAVDSAVSSEHTSEMIQDVDIP-----------GPLETEEVRPMKKAKSEDQ 300

Query: 301 ENCHSDKID---NGACMIDATISEGHTPIDSEVVN-DSV-LPDSDASLKSHPRE-KKLID 360
           +N  +  +    +G  + + T   G+    + V + DS+ + ++D+SLK HPRE KKLID
Sbjct: 301 KNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSIKIVETDSSLKLHPREKKKLID 360

Query: 361 FRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
           FRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF
Sbjct: 361 FRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420

Query: 421 GVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE 480
           GVQICG+YPDTVSR VELID+EC +DFIDINMGCPID+VVNK AGSALL KP+RMK++VE
Sbjct: 421 GVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVE 480

Query: 481 TASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYI 540
            +S IV+TPITIKVRT +FEGKNRIDSLIADIGNWGA AVTIHGR+RQQRYSK ADW+YI
Sbjct: 481 VSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKSADWDYI 540

Query: 541 NRCSSLAPDSLQVIGNGDVFSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNW 600
            +C+  A  +LQVIGNGDV+SY DWN+HK + P+L++CMIARGALIKPW+FTEIKEQR+W
Sbjct: 541 YQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCMIARGALIKPWIFTEIKEQRHW 600

Query: 601 DITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLN 660
           DITSGERLNI+KDFV FGL+HWGSDTKGVETTRHFLLEWLSYT RYIPVGLLDV+PQ++N
Sbjct: 601 DITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEWLSYTFRYIPVGLLDVIPQQIN 660

Query: 661 WRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
           WRPPSYFGRDDLETLM S+SA DW++ISEMLLGKVP+ F+FAPKHKSNAYDRAENG
Sbjct: 661 WRPPSYFGRDDLETLMMSESAGDWVRISEMLLGKVPEGFTFAPKHKSNAYDRAENG 691

BLAST of Carg19376 vs. ExPASy Swiss-Prot
Match: Q7XT07 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0117600 PE=2 SV=2)

HSP 1 Score: 850.1 bits (2195), Expect = 1.7e-245
Identity = 432/682 (63.34%), Postives = 522/682 (76.54%), Query Frame = 0

Query: 20  ASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDTASTGKNDGDDKAAPSS 79
           +S +AS+  + +PEELVA+A+APVK  +LRPP    S    KD    GK +G       +
Sbjct: 19  SSPAASSPPRPSPEELVARAVAPVKPAFLRPP---LSATPPKD---EGKANGG-----GA 78

Query: 80  LVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPAD 139
           +V EKKSKRQ+KRER+QEQKS+SHLC E+ K+G+VSSC+Y D CRFSHDI+A+ +QKPAD
Sbjct: 79  VVAEKKSKRQLKRERKQEQKSSSHLCIEVGKSGNVSSCKYGDSCRFSHDIDAYLAQKPAD 138

Query: 140 LDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCSEMNGLNKDVQKLLWKNKM 199
           L+G CPF+  +QLCPYGL CRF GTHK+   A  +++ K   E+N LNKD+QKLLWKNK 
Sbjct: 139 LEGTCPFTNLDQLCPYGLTCRFLGTHKDIHAASGNLSEK--HEINALNKDIQKLLWKNKY 198

Query: 200 KFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSN--DSAADRTRCGEVNNDMDADLDIPV 259
           KFPKA +++K LGL    KSK     + + V  +  D   D  +    N  ++A+ D   
Sbjct: 199 KFPKASAQIKLLGLKEVIKSKPDAANDDKKVNHDNLDGNDDENKEPLCNPPVNAECD--S 258

Query: 260 EIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACMIDATISEGHTPIDS--- 319
            + EE  ++EG    D S P  +P+        SD+ID        T +E   P  S   
Sbjct: 259 TLCEELDRSEGEPLIDNSIPCVEPRPTKKSKVESDEIDKHGAGTLNTNTESEDPNLSNGL 318

Query: 320 EVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEM 379
           E  N+S    +D     H REKK+IDFR+KLYLAPLTTVGNLPFRR+CK LGAD+TCGEM
Sbjct: 319 EPSNNSSSCRTDLITTPHLREKKIIDFREKLYLAPLTTVGNLPFRRLCKTLGADITCGEM 378

Query: 380 AMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDINMGC 439
           AMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTV+R VEL+D EC +DFIDINMGC
Sbjct: 379 AMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVARTVELVDNECSVDFIDINMGC 438

Query: 440 PIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNRIDSLIADIGN 499
           PIDIVVNKGAGS+LL KPMR+KS+V+ AS + + P+T+KVRT +FEG+NR DS+++DI +
Sbjct: 439 PIDIVVNKGAGSSLLTKPMRIKSIVQAASTVTEKPLTVKVRTAFFEGRNRADSIVSDIYD 498

Query: 500 WGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNRHKVESPK 559
           WGA A+T+HGR+RQQRYSKLADW+YI +C+  APD L V+GNGDVFS+ DWN+H     K
Sbjct: 499 WGASAITVHGRSRQQRYSKLADWDYIYQCAQKAPDQLHVVGNGDVFSFTDWNKHVSGCSK 558

Query: 560 LATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVETTRH 619
           ++T MIARGALIKPW+FTE+KEQR+WDITSGER NILKDFV FGLEHWGSD+KGVETTR+
Sbjct: 559 ISTSMIARGALIKPWIFTEVKEQRHWDITSGERFNILKDFVSFGLEHWGSDSKGVETTRY 618

Query: 620 FLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGK 679
           FLLEWLSYTCRYIPVGLLDV+PQRLNWRPPSY GRDDLETLM SDSAADWI+ISEMLLGK
Sbjct: 619 FLLEWLSYTCRYIPVGLLDVIPQRLNWRPPSYCGRDDLETLMISDSAADWIRISEMLLGK 678

Query: 680 VPDAFSFAPKHKSNAYDRAENG 697
           VP+ F+F PKHKSNAYDRAENG
Sbjct: 679 VPEGFTFTPKHKSNAYDRAENG 685

BLAST of Carg19376 vs. ExPASy Swiss-Prot
Match: Q91XI1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus3l PE=1 SV=1)

HSP 1 Score: 518.8 bits (1335), Expect = 9.2e-146
Identity = 288/686 (41.98%), Postives = 392/686 (57.14%), Query Frame = 0

Query: 38  KAIAPVK----------REYLRPPPVRSSCNNVK--DTASTGK-----NDGDDKAAPSSL 97
           + +AP+K           EYL       +C  V   D A  G       DG +       
Sbjct: 21  RGVAPIKAQYRTTKERFHEYLDGDKQEGACQEVPTGDPAEPGAKRIRLEDGQENGKTEVA 80

Query: 98  VKEKKSKRQMKRERRQEQKSTSH----------LCPEIAKTGDVSSCRYSDKCRFSHDIE 157
           ++ +  +RQ+ +  R + KS  H          LCP   +    + C + D+CRF HD+ 
Sbjct: 81  IESR--ERQVPKRARGQNKSRPHVKPAHYDKDRLCPSFLQE-PATPCAFGDRCRFLHDVG 140

Query: 158 AFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTH--KEGVPAGSSVALKKCSEM----N 217
            +   KPADL   C        CP+ + CRFAG H   EG        + +C+++    N
Sbjct: 141 RYLETKPADLGPRCVLFETFGRCPFSMTCRFAGAHLGPEGQNLVQEEVVARCAQLPSVRN 200

Query: 218 GLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRTRCGE 277
           GL++ +Q+ L K ++ F +A+  L  L                                 
Sbjct: 201 GLDRALQQQLRKRQVCFERAEQALNRL--------------------------------- 260

Query: 278 VNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACMIDAT 337
                     +P  +PE             +  +  PK  S   CH+ ++D        T
Sbjct: 261 ------TQSPMPTVVPE------------TTVAMATPKQNS---CHA-QLD--------T 320

Query: 338 ISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 397
           +    TP  S V     L D D  ++  P EKK +D   KLYLAPLTT GNLPFRR+CK 
Sbjct: 321 VGGAGTPQSSPVPTCGPLTDEDV-IRLRPCEKKRLDISGKLYLAPLTTCGNLPFRRICKR 380

Query: 398 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 457
            GADVTCGEMA+CTNLLQGQ SEWALL+RH  ED+FGVQ+ GA+PDT+++  EL+++   
Sbjct: 381 FGADVTCGEMAVCTNLLQGQMSEWALLKRHPCEDIFGVQLEGAFPDTMTKCAELLNRTID 440

Query: 458 IDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNR 517
           +DF+DIN+GCPID+V  KG G AL+ +  + + +V   +G++D P+T+K+RTG  E  + 
Sbjct: 441 VDFVDINVGCPIDLVYKKGGGCALMNRSAKFQQIVRGVNGVLDVPLTVKMRTGVQERVSL 500

Query: 518 IDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAD 577
              L+ ++ +WG   VT+HGR+R+QRY++LADW YI +C+ +A   + + GNGD+ S+ D
Sbjct: 501 AHRLLPELRDWGVALVTLHGRSREQRYTRLADWPYIEQCAKVA-SPMPLFGNGDILSFED 560

Query: 578 WNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 637
            N        +A  M+ARGAL+KPWLFTEIKEQR+WDI+S ERL+IL+DF H+GLEHWGS
Sbjct: 561 AN--CAMQTGVAGIMVARGALLKPWLFTEIKEQRHWDISSSERLDILRDFTHYGLEHWGS 620

Query: 638 DTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADW 691
           DT+GVE TR FLLEWLS+ CRY+PVGLL+  PQR+N RPP Y GRD LETLM+S  AADW
Sbjct: 621 DTQGVERTRRFLLEWLSFLCRYVPVGLLERPPQRINERPPYYLGRDYLETLMASQQAADW 636

BLAST of Carg19376 vs. ExPASy Swiss-Prot
Match: Q96G46 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS3L PE=1 SV=2)

HSP 1 Score: 518.1 bits (1333), Expect = 1.6e-145
Identity = 279/640 (43.59%), Postives = 377/640 (58.91%), Query Frame = 0

Query: 62  DTASTGKNDGDDKAAPSSLVKEKKSKRQMKRER---RQEQKSTSHLCPEIAKTGDVSSCR 121
           +   T     ++ A P   ++ +K  R   + R   +      + LCP + +    + C 
Sbjct: 78  EDGQTADGQTEEAAEPGEQLQTQKRARGQNKGRPHVKPTNYDKNRLCPSLIQE-SAAKCF 137

Query: 122 YSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALK 181
           + D+CRF HD+  +   KPADL   C        CPYG+ CRFAG H    P G ++  +
Sbjct: 138 FGDRCRFLHDVGRYLETKPADLGPRCVLFETFGRCPYGVTCRFAGAHLR--PEGQNLVQE 197

Query: 182 KC--------SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDV 241
           +         S  NGL+K +Q+ L K +++F +A+  L+       G + +         
Sbjct: 198 ELAARGTQPPSIRNGLDKALQQQLRKREVRFERAEQALRR---FSQGPTPA--------- 257

Query: 242 VSNDSAADRTRCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCH 301
                                       +P      EGT +    R          ENC 
Sbjct: 258 --------------------------AAVP------EGTAAEGAPR---------QENCG 317

Query: 302 SDKIDNGACMIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPL 361
           + ++  G            TP  S V     L D D  ++  P EKK +D R KLYLAPL
Sbjct: 318 AQQVPAG--------PGTSTPPSSPVRTCGPLTDEDV-VRLRPCEKKRLDIRGKLYLAPL 377

Query: 362 TTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPD 421
           TT GNLPFRR+CK  GADVTCGEMA+CTNLLQGQ SEWALL+RH  ED+FGVQ+ GA+PD
Sbjct: 378 TTCGNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHQCEDIFGVQLEGAFPD 437

Query: 422 TVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPI 481
           T+++  EL+ +   +DF+DIN+GCPID+V  KG G AL+ +  + + +V   + ++D P+
Sbjct: 438 TMTKCAELLSRTVEVDFVDINVGCPIDLVYKKGGGCALMNRSTKFQQIVRGMNQVLDVPL 497

Query: 482 TIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDS 541
           T+K+RTG  E  N    L+ ++ +WG   VT+HGR+R+QRY+KLADW+YI  C   A   
Sbjct: 498 TVKIRTGVQERVNLAHRLLPELRDWGVALVTLHGRSREQRYTKLADWQYIEECVQ-AASP 557

Query: 542 LQVIGNGDVFSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNI 601
           + + GNGD+ S+ D NR       +   MIARGAL+KPWLFTEIKEQR+WDI+S ERL+I
Sbjct: 558 MPLFGNGDILSFEDANR--AMQTGVTGIMIARGALLKPWLFTEIKEQRHWDISSSERLDI 617

Query: 602 LKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRD 661
           L+DF ++GLEHWGSDT+GVE TR FLLEWLS+ CRY+PVGLL+ +PQR+N RPP Y GRD
Sbjct: 618 LRDFTNYGLEHWGSDTQGVEKTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLGRD 649

Query: 662 DLETLMSSDSAADWIKISEMLLGKVPDAFSFAPKHKSNAY 691
            LETLM+S  AADWI+ISEMLLG VP +F+F PKHK+NAY
Sbjct: 678 YLETLMASQKAADWIRISEMLLGPVPPSFAFLPKHKANAY 649

BLAST of Carg19376 vs. ExPASy Swiss-Prot
Match: Q7ZWS1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Xenopus laevis OX=8355 GN=dus3l PE=2 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.7e-144
Identity = 279/638 (43.73%), Postives = 376/638 (58.93%), Query Frame = 0

Query: 67  GKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKST----SHLCPEIAKTGDVSSCRYSDK 126
           G ++G D+   ++  K++K + + + + R   K +    + LCP + +    S C + DK
Sbjct: 87  GSSEGQDQPPKTAENKQEKKRARGQNKSRPHMKHSQFEENKLCPSVTQE-CASKCFFGDK 146

Query: 127 CRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTH----------KEGVPAG 186
           C+FSHD+  + SQKP D+   C        C YG+ CRFA +H          +E +   
Sbjct: 147 CKFSHDVAKYVSQKPEDIRPNCHLYETFGKCIYGVTCRFAKSHLGEDFKNIINEELMKEW 206

Query: 187 SSVALKKCSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVS 246
               L K    N L+K +++ L K K+ F K+D  LK     G         + K  VV 
Sbjct: 207 EGKVLVK----NSLDKSLKEQLRKKKVVFEKSDKYLKLCFKSGDS------SKMKNPVVK 266

Query: 247 NDSAADRTRCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSD 306
            DSA   T+                                                   
Sbjct: 267 EDSAVQVTQ--------------------------------------------------- 326

Query: 307 KIDNGACMIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTT 366
                               DS +     + D D  +K  P EKK IDFR+KLYLAPLTT
Sbjct: 327 -------------------KDSPITTVGAVTDEDV-IKLRPCEKKTIDFRNKLYLAPLTT 386

Query: 367 VGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTV 426
            GNLPFRR+CK LGAD+TCGEMAMCTNLLQGQ SEWALL+RH SED+FGVQ+ GA+PDT+
Sbjct: 387 CGNLPFRRICKRLGADITCGEMAMCTNLLQGQPSEWALLKRHHSEDIFGVQLEGAFPDTM 446

Query: 427 SRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITI 486
           ++  EL+++   +DF+DIN+GCPID+V  KG G  L+ +  + + +V+  + ++D P+T+
Sbjct: 447 TKCAELLNRTIDVDFVDINVGCPIDLVYKKGGGCGLMNRTNKFEQIVKGMNSVLDVPLTV 506

Query: 487 KVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQ 546
           K+RTG  E  N    LI ++ +WG   VT+HGR+R+QRY+KLA+WEYI++C+ +A   + 
Sbjct: 507 KIRTGVQEKVNIAHKLIPNLRDWGVSLVTLHGRSREQRYTKLANWEYIDQCAKIA-SPVP 566

Query: 547 VIGNGDVFSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILK 606
           + GNGD+ SY D NR       +A  M+ARGAL KPWLFTEIKEQR+WDI+S ER +ILK
Sbjct: 567 LFGNGDIISYEDANR--ALQTGVAGVMLARGALFKPWLFTEIKEQRHWDISSTERFDILK 626

Query: 607 DFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDL 666
           DF ++GLEHWGSD +GVE TR F+LEWLS+ CRYIPVGLL+ VPQ++N RPP Y GRD L
Sbjct: 627 DFTNYGLEHWGSDCQGVEKTRKFMLEWLSFLCRYIPVGLLEHVPQKINERPPYYMGRDYL 639

Query: 667 ETLMSSDSAADWIKISEMLLGKVPDAFSFAPKHKSNAY 691
           ETLM+S +  DWIKISEMLLG VP  F+F PKHK+N+Y
Sbjct: 687 ETLMASQNVTDWIKISEMLLGPVPPNFTFLPKHKANSY 639

BLAST of Carg19376 vs. ExPASy TrEMBL
Match: A0A6J1GL24 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita moschata OX=3662 GN=LOC111455351 PE=3 SV=1)

HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 693/696 (99.57%), Postives = 695/696 (99.86%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPP+RSSCNNV
Sbjct: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPMRSSCNNV 60

Query: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240

Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
           RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300

Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
           IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360

Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420

Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
           KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480

Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
           GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540

Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
           SYADWNRHKVESPKLATC IARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGLE
Sbjct: 541 SYADWNRHKVESPKLATCTIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGLE 600

Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
           HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660

Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
           AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696

BLAST of Carg19376 vs. ExPASy TrEMBL
Match: A0A6J1HXQ8 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita maxima OX=3661 GN=LOC111468431 PE=3 SV=1)

HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 681/696 (97.84%), Postives = 687/696 (98.71%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
           MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60

Query: 61  KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
           KD ASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61  KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120

Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
           DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180

Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
           SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240

Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
            C EVNNDMDADLDIP+E+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDNGACM
Sbjct: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGACM 300

Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
           IDATI EGHTPI+SEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360

Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420

Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
           KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFE 480

Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
           GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540

Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
           SYA+WNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE
Sbjct: 541 SYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600

Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
           HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660

Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
           AADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696

BLAST of Carg19376 vs. ExPASy TrEMBL
Match: A0A0A0K4I1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7G007900 PE=3 SV=1)

HSP 1 Score: 1271.5 bits (3289), Expect = 0.0e+00
Identity = 624/696 (89.66%), Postives = 655/696 (94.11%), Query Frame = 0

Query: 2   EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61
           EDSV+A+L D TAVSE P S S+  G  LTP++LVAKAIAPVKR++LRPPP+RSS NN+K
Sbjct: 3   EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNLK 62

Query: 62  DTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSD 121
           D  ST KNDGDDKAAPS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDVSSCRYSD
Sbjct: 63  DATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSD 122

Query: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCS 181
           KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LK+CS
Sbjct: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182

Query: 182 EMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDS-AADRT 241
           EMNGLNKD+QKLLWKNKMKFPKADSKLKELGLLGHGKSK RVKE++E+ VSNDS  ADR 
Sbjct: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242

Query: 242 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 301
            C E+NND+DADL+I VE+PEENG+ EGTFSSDESRPLKK KS+SSEN HSD+IDNGACM
Sbjct: 243 CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACM 302

Query: 302 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 361
           ID TI EGH PI+SE +NDSVL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 303 IDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRR 362

Query: 362 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 421
           VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 363 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 422

Query: 422 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 481
           KECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+E
Sbjct: 423 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYE 482

Query: 482 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 541
           GKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 483 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 542

Query: 542 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 601
           SY DWN HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGLE
Sbjct: 543 SYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLE 602

Query: 602 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 661
           HWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 603 HWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDS 662

Query: 662 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
           AADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 663 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of Carg19376 vs. ExPASy TrEMBL
Match: A0A5D3CK47 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G001380 PE=3 SV=1)

HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 615/685 (89.78%), Postives = 643/685 (93.87%), Query Frame = 0

Query: 13  TAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDTASTGKNDGD 72
           TAVSE P S SA  G   TP++LVAKAIAPV  ++LRPPP+RSS N +KD  ST  NDGD
Sbjct: 7   TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNTLKDATSTANNDGD 66

Query: 73  DKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAF 132
           DKA PS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDV+SCRYSDKCRFSHDIEAF
Sbjct: 67  DKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFSHDIEAF 126

Query: 133 KSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCSEMNGLNKDVQK 192
           KSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LK+CSEMNGLNKD+QK
Sbjct: 127 KSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQK 186

Query: 193 LLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDS-AADRTRCGEVNNDMDA 252
           LLWKNKMKFPKADSKLKELGLLGHGKSK RVKE+KE+ VSNDS  ADRT C E NND+DA
Sbjct: 187 LLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSETNNDLDA 246

Query: 253 DLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACMIDATISEGHTP 312
           DL+IPVE+PEENG+ EGTFSSDESRPLKK KS+SS N HSD+IDNGACMID TI EGH P
Sbjct: 247 DLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGACMIDETIGEGHAP 306

Query: 313 IDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC 372
           I+SEV+ND VL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC
Sbjct: 307 IESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC 366

Query: 373 GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDIN 432
           GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV+DFIDIN
Sbjct: 367 GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDIN 426

Query: 433 MGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNRIDSLIAD 492
           MGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNRIDSLIAD
Sbjct: 427 MGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIAD 486

Query: 493 IGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNRHKVE 552
           IGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWN+HK E
Sbjct: 487 IGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNQHKDE 546

Query: 553 SPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVET 612
            PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSD+KGVET
Sbjct: 547 CPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDSKGVET 606

Query: 613 TRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML 672
           TRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML
Sbjct: 607 TRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML 666

Query: 673 LGKVPDAFSFAPKHKSNAYDRAENG 697
           LGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 667 LGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of Carg19376 vs. ExPASy TrEMBL
Match: A0A1S3BIS3 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo OX=3656 GN=LOC103490332 PE=3 SV=1)

HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 615/685 (89.78%), Postives = 643/685 (93.87%), Query Frame = 0

Query: 13  TAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDTASTGKNDGD 72
           TAVSE P S SA  G   TP++LVAKAIAPV  ++LRPPP+RSS N +KD  ST  NDGD
Sbjct: 7   TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNTLKDATSTANNDGD 66

Query: 73  DKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAF 132
           DKA PS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDV+SCRYSDKCRFSHDIEAF
Sbjct: 67  DKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFSHDIEAF 126

Query: 133 KSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCSEMNGLNKDVQK 192
           KSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LK+CSEMNGLNKD+QK
Sbjct: 127 KSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQK 186

Query: 193 LLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDS-AADRTRCGEVNNDMDA 252
           LLWKNKMKFPKADSKLKELGLLGHGKSK RVKE+KE+ VSNDS  ADRT C E NND+DA
Sbjct: 187 LLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSETNNDLDA 246

Query: 253 DLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACMIDATISEGHTP 312
           DL+IPVE+PEENG+ EGTFSSDESRPLKK KS+SS N HSD+IDNGACMID TI EGH P
Sbjct: 247 DLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGACMIDETIGEGHAP 306

Query: 313 IDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC 372
           I+SEV+ND VL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC
Sbjct: 307 IESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC 366

Query: 373 GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDIN 432
           GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV+DFIDIN
Sbjct: 367 GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDIN 426

Query: 433 MGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNRIDSLIAD 492
           MGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNRIDSLIAD
Sbjct: 427 MGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIAD 486

Query: 493 IGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNRHKVE 552
           IGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWN+HK E
Sbjct: 487 IGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNQHKDE 546

Query: 553 SPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVET 612
            PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSD+KGVET
Sbjct: 547 CPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDSKGVET 606

Query: 613 TRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML 672
           TRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML
Sbjct: 607 TRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML 666

Query: 673 LGKVPDAFSFAPKHKSNAYDRAENG 697
           LGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 667 LGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of Carg19376 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 869.8 bits (2246), Expect = 1.5e-252
Identity = 447/716 (62.43%), Postives = 544/716 (75.98%), Query Frame = 0

Query: 1   MEDSVSAELRDPTAVSEEPASLSASTGAQL-----TPEELVAKAIAPVKREYLRPPPVRS 60
           M D ++    D +   +  A+L   +  Q      T EEL+ +++AP+K+E+L PPP   
Sbjct: 1   MSDVIAEHAVDTSVEGDAKAALPVQSTKQFSVYEATSEELIERSMAPIKKEFLCPPP--- 60

Query: 61  SCNNVKDTASTGKNDGDDKAAP-SSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDV 120
                  + S  +ND  D  AP S LV+EKKSKRQ+KRERR++  ST +LCP++A+T DV
Sbjct: 61  ------PSRSVKQNDAADVRAPQSGLVQEKKSKRQLKRERREQ--STINLCPQVARTEDV 120

Query: 121 SSCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSS 180
            SC+Y DKCRF+HDIEAFK+QK  D++G+CPF  +   C YGL+CRF G+H++    G+S
Sbjct: 121 DSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASGMKCAYGLSCRFLGSHRD--ITGNS 180

Query: 181 VALKKCSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSN- 240
              +K SEMN  NK+ Q+LLWKNKM F  AD+KLK LGLLGH K  +  +E   +   N 
Sbjct: 181 DDKEK-SEMNFFNKETQRLLWKNKMTFTNADAKLKSLGLLGHAKKSNAAEEITAEKTQNG 240

Query: 241 -------DSAADRTRCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSS 300
                  + A D     E  ++M  D+DIP           G   ++E RP+KK KS   
Sbjct: 241 MNGTQATEVAVDSAVSSEHTSEMIQDVDIP-----------GPLETEEVRPMKKAKSEDQ 300

Query: 301 ENCHSDKID---NGACMIDATISEGHTPIDSEVVN-DSV-LPDSDASLKSHPRE-KKLID 360
           +N  +  +    +G  + + T   G+    + V + DS+ + ++D+SLK HPRE KKLID
Sbjct: 301 KNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSIKIVETDSSLKLHPREKKKLID 360

Query: 361 FRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
           FRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF
Sbjct: 361 FRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420

Query: 421 GVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE 480
           GVQICG+YPDTVSR VELID+EC +DFIDINMGCPID+VVNK AGSALL KP+RMK++VE
Sbjct: 421 GVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVE 480

Query: 481 TASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYI 540
            +S IV+TPITIKVRT +FEGKNRIDSLIADIGNWGA AVTIHGR+RQQRYSK ADW+YI
Sbjct: 481 VSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKSADWDYI 540

Query: 541 NRCSSLAPDSLQVIGNGDVFSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNW 600
            +C+  A  +LQVIGNGDV+SY DWN+HK + P+L++CMIARGALIKPW+FTEIKEQR+W
Sbjct: 541 YQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCMIARGALIKPWIFTEIKEQRHW 600

Query: 601 DITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLN 660
           DITSGERLNI+KDFV FGL+HWGSDTKGVETTRHFLLEWLSYT RYIPVGLLDV+PQ++N
Sbjct: 601 DITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEWLSYTFRYIPVGLLDVIPQQIN 660

Query: 661 WRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
           WRPPSYFGRDDLETLM S+SA DW++ISEMLLGKVP+ F+FAPKHKSNAYDRAENG
Sbjct: 661 WRPPSYFGRDDLETLMMSESAGDWVRISEMLLGKVPEGFTFAPKHKSNAYDRAENG 691

BLAST of Carg19376 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 110.9 bits (276), Expect = 4.1e-24
Identity = 87/309 (28.16%), Postives = 145/309 (46.93%), Query Frame = 0

Query: 339 IDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGE------MAMCTNLLQGQASEWALLR 398
           +D+++KL LAP+  VG L FR +    GAD+T GE      +  C   L   +     + 
Sbjct: 1   MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60

Query: 399 RHSSEDLFGVQICGAYPDTV---------SRAVELIDKEC-VIDFIDINMGCPIDIVVNK 458
           + +   +F    C      V          RA++  +  C  +  IDINMGCP    +  
Sbjct: 61  KGTDNVVFST--CDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQG 120

Query: 459 GAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTI 518
           G G+ALL KP  +  ++ T    +D P+T K+R    +       L   I   G  A+ +
Sbjct: 121 GMGAALLSKPELIHDILATLKRNLDVPVTCKIR--LLKSPADTVELARRIEKLGVPALAV 180

Query: 519 HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNRHKVESPKLATCMIAR 578
           HGR    R    A W+ I     +A  S+ VI NGDV  Y D++R K  +   A+ M+AR
Sbjct: 181 HGRKIADRPRDPAKWDEI--ADVVAALSIPVIANGDVLEYDDFSRIKT-ATGAASVMVAR 240

Query: 579 GALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLS- 631
           GA+    +F+  K + +W+       ++ K ++   +  W +D   V++T++ + E ++ 
Sbjct: 241 GAMWNASIFSP-KGKSHWE-------DVKKKYLRKSI-LWNND---VKSTKYTIKEMIAH 290

BLAST of Carg19376 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 77.4 bits (189), Expect = 5.0e-14
Identity = 58/204 (28.43%), Postives = 95/204 (46.57%), Query Frame = 0

Query: 342 RDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEM---AMCTNLLQGQASEWALLRRHSSED 401
           R K  +AP+     LPFR +C+  GA      M    + T   + +  E+   +      
Sbjct: 87  RPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQEFTTCK--EDRP 146

Query: 402 LFGVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSV 461
           LF VQ C   PDT+  A + ++  C  D++DIN+GCP  I      G+ L+     +KS+
Sbjct: 147 LF-VQFCANDPDTLLEAAKRVEPYC--DYVDINLGCPQRIARRGNYGAFLMDNLPLVKSL 206

Query: 462 VETASGIVDTPITIKVRT-GYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSK--LA 521
           VE  +  ++ P++ K+R     E   +   ++ D    G   + +HGRTR ++  K   A
Sbjct: 207 VEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDA---GCSLLAVHGRTRDEKDGKKFRA 266

Query: 522 DWEYINRCSSLAPDSLQVIGNGDV 540
           DW  I    +     + V+ NG+V
Sbjct: 267 DWSAIKEVKNAM--RIPVLANGNV 280

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6572077.10.0e+00100.00tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein, partial [Cucurbita ar... [more]
XP_022952746.10.0e+0099.57tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita moschata][more]
XP_022969416.10.0e+0097.84tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima][more]
XP_023554076.10.0e+0097.70tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo][more]
XP_004148548.10.0e+0089.66tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 ... [more]
Match NameE-valueIdentityDescription
Q9T0J62.1e-25162.43tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=370... [more]
Q7XT071.7e-24563.34tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonic... [more]
Q91XI19.2e-14641.98tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Du... [more]
Q96G461.6e-14543.59tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS... [more]
Q7ZWS11.7e-14443.73tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Xenopus laevis OX=8355 GN=d... [more]
Match NameE-valueIdentityDescription
A0A6J1GL240.0e+0099.57tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1HXQ80.0e+0097.84tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0K4I10.0e+0089.66tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7... [more]
A0A5D3CK470.0e+0089.78tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3BIS30.0e+0089.78tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo OX=3656 GN=LOC10349... [more]
Match NameE-valueIdentityDescription
AT4G38890.11.5e-25262.43FMN-linked oxidoreductases superfamily protein [more]
AT3G49640.14.1e-2428.16Aldolase-type TIM barrel family protein [more]
AT5G67220.15.0e-1428.43FMN-linked oxidoreductases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 340..582
e-value: 3.3E-72
score: 244.6
IPR035587DUS-like, FMN-binding domainPFAMPF01207Duscoord: 347..614
e-value: 4.1E-49
score: 167.3
IPR035587DUS-like, FMN-binding domainCDDcd02801DUS_like_FMNcoord: 344..578
e-value: 8.6426E-91
score: 280.535
NoneNo IPR availableGENE3D4.10.1000.10coord: 94..173
e-value: 6.0E-6
score: 28.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..105
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 217..242
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 273..289
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 260..291
NoneNo IPR availablePANTHERPTHR45846:SF1TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKEcoord: 39..696
NoneNo IPR availablePANTHERPTHR45846TRNA-DIHYDROURIDINE(47) SYNTHASE [NAD(P)(+)]-LIKEcoord: 39..696
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 344..657
IPR041367E3 ligase, CCCH-type zinc fingerPFAMPF18044zf-CCCH_4coord: 103..128
e-value: 6.7E-6
score: 25.7
IPR018517tRNA-dihydrouridine synthase, conserved sitePROSITEPS01136UPF0034coord: 428..446
IPR000571Zinc finger, CCCH-typePROSITEPS50103ZF_C3H1coord: 99..130
score: 10.727612

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19376-RACarg19376-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002943 tRNA dihydrouridine synthesis
biological_process GO:0008033 tRNA processing
molecular_function GO:0003677 DNA binding
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0017150 tRNA dihydrouridine synthase activity
molecular_function GO:0003824 catalytic activity