Homology
BLAST of Carg19376 vs. NCBI nr
Match:
KAG6572077.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011741.1 tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1404.4 bits (3634), Expect = 0.0e+00
Identity = 696/696 (100.00%), Postives = 696/696 (100.00%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
Query: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE
Sbjct: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696
BLAST of Carg19376 vs. NCBI nr
Match:
XP_022952746.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita moschata])
HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 693/696 (99.57%), Postives = 695/696 (99.86%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPP+RSSCNNV
Sbjct: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPMRSSCNNV 60
Query: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
SYADWNRHKVESPKLATC IARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGLE
Sbjct: 541 SYADWNRHKVESPKLATCTIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGLE 600
Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696
BLAST of Carg19376 vs. NCBI nr
Match:
XP_022969416.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima])
HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 681/696 (97.84%), Postives = 687/696 (98.71%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
Query: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KD ASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
C EVNNDMDADLDIP+E+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDNGACM
Sbjct: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGACM 300
Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
IDATI EGHTPI+SEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFE 480
Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
SYA+WNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE
Sbjct: 541 SYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
AADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696
BLAST of Carg19376 vs. NCBI nr
Match:
XP_023554076.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1372.8 bits (3552), Expect = 0.0e+00
Identity = 680/696 (97.70%), Postives = 687/696 (98.71%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELR PTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSS NNV
Sbjct: 1 MEDSVSAELRVPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSGNNV 60
Query: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KD ASTGKNDGD+KAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDAASTGKNDGDNKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV+LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVSLKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
C EVNNDMDADLDIPVE+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM
Sbjct: 241 CCSEVNNDMDADLDIPVEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
IDATISEGHTPI+SEVVND VLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATISEGHTPIESEVVNDIVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
GKNRIDSLIADIGNWGA AVT+HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGASAVTVHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGLE
Sbjct: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGLE 600
Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696
BLAST of Carg19376 vs. NCBI nr
Match:
XP_004148548.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 hypothetical protein Csa_020245 [Cucumis sativus])
HSP 1 Score: 1271.5 bits (3289), Expect = 0.0e+00
Identity = 624/696 (89.66%), Postives = 655/696 (94.11%), Query Frame = 0
Query: 2 EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61
EDSV+A+L D TAVSE P S S+ G LTP++LVAKAIAPVKR++LRPPP+RSS NN+K
Sbjct: 3 EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNLK 62
Query: 62 DTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSD 121
D ST KNDGDDKAAPS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDVSSCRYSD
Sbjct: 63 DATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSD 122
Query: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCS 181
KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LK+CS
Sbjct: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182
Query: 182 EMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDS-AADRT 241
EMNGLNKD+QKLLWKNKMKFPKADSKLKELGLLGHGKSK RVKE++E+ VSNDS ADR
Sbjct: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242
Query: 242 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 301
C E+NND+DADL+I VE+PEENG+ EGTFSSDESRPLKK KS+SSEN HSD+IDNGACM
Sbjct: 243 CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACM 302
Query: 302 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 361
ID TI EGH PI+SE +NDSVL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 303 IDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRR 362
Query: 362 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 421
VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 363 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 422
Query: 422 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 481
KECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+E
Sbjct: 423 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYE 482
Query: 482 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 541
GKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 483 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 542
Query: 542 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 601
SY DWN HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGLE
Sbjct: 543 SYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLE 602
Query: 602 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 661
HWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 603 HWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDS 662
Query: 662 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
AADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 663 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
BLAST of Carg19376 vs. ExPASy Swiss-Prot
Match:
Q9T0J6 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=3702 GN=At4g38890 PE=1 SV=2)
HSP 1 Score: 869.8 bits (2246), Expect = 2.1e-251
Identity = 447/716 (62.43%), Postives = 544/716 (75.98%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQL-----TPEELVAKAIAPVKREYLRPPPVRS 60
M D ++ D + + A+L + Q T EEL+ +++AP+K+E+L PPP
Sbjct: 1 MSDVIAEHAVDTSVEGDAKAALPVQSTKQFSVYEATSEELIERSMAPIKKEFLCPPP--- 60
Query: 61 SCNNVKDTASTGKNDGDDKAAP-SSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDV 120
+ S +ND D AP S LV+EKKSKRQ+KRERR++ ST +LCP++A+T DV
Sbjct: 61 ------PSRSVKQNDAADVRAPQSGLVQEKKSKRQLKRERREQ--STINLCPQVARTEDV 120
Query: 121 SSCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSS 180
SC+Y DKCRF+HDIEAFK+QK D++G+CPF + C YGL+CRF G+H++ G+S
Sbjct: 121 DSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASGMKCAYGLSCRFLGSHRD--ITGNS 180
Query: 181 VALKKCSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSN- 240
+K SEMN NK+ Q+LLWKNKM F AD+KLK LGLLGH K + +E + N
Sbjct: 181 DDKEK-SEMNFFNKETQRLLWKNKMTFTNADAKLKSLGLLGHAKKSNAAEEITAEKTQNG 240
Query: 241 -------DSAADRTRCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSS 300
+ A D E ++M D+DIP G ++E RP+KK KS
Sbjct: 241 MNGTQATEVAVDSAVSSEHTSEMIQDVDIP-----------GPLETEEVRPMKKAKSEDQ 300
Query: 301 ENCHSDKID---NGACMIDATISEGHTPIDSEVVN-DSV-LPDSDASLKSHPRE-KKLID 360
+N + + +G + + T G+ + V + DS+ + ++D+SLK HPRE KKLID
Sbjct: 301 KNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSIKIVETDSSLKLHPREKKKLID 360
Query: 361 FRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
FRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF
Sbjct: 361 FRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
Query: 421 GVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE 480
GVQICG+YPDTVSR VELID+EC +DFIDINMGCPID+VVNK AGSALL KP+RMK++VE
Sbjct: 421 GVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVE 480
Query: 481 TASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYI 540
+S IV+TPITIKVRT +FEGKNRIDSLIADIGNWGA AVTIHGR+RQQRYSK ADW+YI
Sbjct: 481 VSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKSADWDYI 540
Query: 541 NRCSSLAPDSLQVIGNGDVFSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNW 600
+C+ A +LQVIGNGDV+SY DWN+HK + P+L++CMIARGALIKPW+FTEIKEQR+W
Sbjct: 541 YQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCMIARGALIKPWIFTEIKEQRHW 600
Query: 601 DITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLN 660
DITSGERLNI+KDFV FGL+HWGSDTKGVETTRHFLLEWLSYT RYIPVGLLDV+PQ++N
Sbjct: 601 DITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEWLSYTFRYIPVGLLDVIPQQIN 660
Query: 661 WRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
WRPPSYFGRDDLETLM S+SA DW++ISEMLLGKVP+ F+FAPKHKSNAYDRAENG
Sbjct: 661 WRPPSYFGRDDLETLMMSESAGDWVRISEMLLGKVPEGFTFAPKHKSNAYDRAENG 691
BLAST of Carg19376 vs. ExPASy Swiss-Prot
Match:
Q7XT07 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0117600 PE=2 SV=2)
HSP 1 Score: 850.1 bits (2195), Expect = 1.7e-245
Identity = 432/682 (63.34%), Postives = 522/682 (76.54%), Query Frame = 0
Query: 20 ASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDTASTGKNDGDDKAAPSS 79
+S +AS+ + +PEELVA+A+APVK +LRPP S KD GK +G +
Sbjct: 19 SSPAASSPPRPSPEELVARAVAPVKPAFLRPP---LSATPPKD---EGKANGG-----GA 78
Query: 80 LVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPAD 139
+V EKKSKRQ+KRER+QEQKS+SHLC E+ K+G+VSSC+Y D CRFSHDI+A+ +QKPAD
Sbjct: 79 VVAEKKSKRQLKRERKQEQKSSSHLCIEVGKSGNVSSCKYGDSCRFSHDIDAYLAQKPAD 138
Query: 140 LDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCSEMNGLNKDVQKLLWKNKM 199
L+G CPF+ +QLCPYGL CRF GTHK+ A +++ K E+N LNKD+QKLLWKNK
Sbjct: 139 LEGTCPFTNLDQLCPYGLTCRFLGTHKDIHAASGNLSEK--HEINALNKDIQKLLWKNKY 198
Query: 200 KFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSN--DSAADRTRCGEVNNDMDADLDIPV 259
KFPKA +++K LGL KSK + + V + D D + N ++A+ D
Sbjct: 199 KFPKASAQIKLLGLKEVIKSKPDAANDDKKVNHDNLDGNDDENKEPLCNPPVNAECD--S 258
Query: 260 EIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACMIDATISEGHTPIDS--- 319
+ EE ++EG D S P +P+ SD+ID T +E P S
Sbjct: 259 TLCEELDRSEGEPLIDNSIPCVEPRPTKKSKVESDEIDKHGAGTLNTNTESEDPNLSNGL 318
Query: 320 EVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEM 379
E N+S +D H REKK+IDFR+KLYLAPLTTVGNLPFRR+CK LGAD+TCGEM
Sbjct: 319 EPSNNSSSCRTDLITTPHLREKKIIDFREKLYLAPLTTVGNLPFRRLCKTLGADITCGEM 378
Query: 380 AMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDINMGC 439
AMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTV+R VEL+D EC +DFIDINMGC
Sbjct: 379 AMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVARTVELVDNECSVDFIDINMGC 438
Query: 440 PIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNRIDSLIADIGN 499
PIDIVVNKGAGS+LL KPMR+KS+V+ AS + + P+T+KVRT +FEG+NR DS+++DI +
Sbjct: 439 PIDIVVNKGAGSSLLTKPMRIKSIVQAASTVTEKPLTVKVRTAFFEGRNRADSIVSDIYD 498
Query: 500 WGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNRHKVESPK 559
WGA A+T+HGR+RQQRYSKLADW+YI +C+ APD L V+GNGDVFS+ DWN+H K
Sbjct: 499 WGASAITVHGRSRQQRYSKLADWDYIYQCAQKAPDQLHVVGNGDVFSFTDWNKHVSGCSK 558
Query: 560 LATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVETTRH 619
++T MIARGALIKPW+FTE+KEQR+WDITSGER NILKDFV FGLEHWGSD+KGVETTR+
Sbjct: 559 ISTSMIARGALIKPWIFTEVKEQRHWDITSGERFNILKDFVSFGLEHWGSDSKGVETTRY 618
Query: 620 FLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEMLLGK 679
FLLEWLSYTCRYIPVGLLDV+PQRLNWRPPSY GRDDLETLM SDSAADWI+ISEMLLGK
Sbjct: 619 FLLEWLSYTCRYIPVGLLDVIPQRLNWRPPSYCGRDDLETLMISDSAADWIRISEMLLGK 678
Query: 680 VPDAFSFAPKHKSNAYDRAENG 697
VP+ F+F PKHKSNAYDRAENG
Sbjct: 679 VPEGFTFTPKHKSNAYDRAENG 685
BLAST of Carg19376 vs. ExPASy Swiss-Prot
Match:
Q91XI1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus3l PE=1 SV=1)
HSP 1 Score: 518.8 bits (1335), Expect = 9.2e-146
Identity = 288/686 (41.98%), Postives = 392/686 (57.14%), Query Frame = 0
Query: 38 KAIAPVK----------REYLRPPPVRSSCNNVK--DTASTGK-----NDGDDKAAPSSL 97
+ +AP+K EYL +C V D A G DG +
Sbjct: 21 RGVAPIKAQYRTTKERFHEYLDGDKQEGACQEVPTGDPAEPGAKRIRLEDGQENGKTEVA 80
Query: 98 VKEKKSKRQMKRERRQEQKSTSH----------LCPEIAKTGDVSSCRYSDKCRFSHDIE 157
++ + +RQ+ + R + KS H LCP + + C + D+CRF HD+
Sbjct: 81 IESR--ERQVPKRARGQNKSRPHVKPAHYDKDRLCPSFLQE-PATPCAFGDRCRFLHDVG 140
Query: 158 AFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTH--KEGVPAGSSVALKKCSEM----N 217
+ KPADL C CP+ + CRFAG H EG + +C+++ N
Sbjct: 141 RYLETKPADLGPRCVLFETFGRCPFSMTCRFAGAHLGPEGQNLVQEEVVARCAQLPSVRN 200
Query: 218 GLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRTRCGE 277
GL++ +Q+ L K ++ F +A+ L L
Sbjct: 201 GLDRALQQQLRKRQVCFERAEQALNRL--------------------------------- 260
Query: 278 VNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACMIDAT 337
+P +PE + + PK S CH+ ++D T
Sbjct: 261 ------TQSPMPTVVPE------------TTVAMATPKQNS---CHA-QLD--------T 320
Query: 338 ISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 397
+ TP S V L D D ++ P EKK +D KLYLAPLTT GNLPFRR+CK
Sbjct: 321 VGGAGTPQSSPVPTCGPLTDEDV-IRLRPCEKKRLDISGKLYLAPLTTCGNLPFRRICKR 380
Query: 398 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 457
GADVTCGEMA+CTNLLQGQ SEWALL+RH ED+FGVQ+ GA+PDT+++ EL+++
Sbjct: 381 FGADVTCGEMAVCTNLLQGQMSEWALLKRHPCEDIFGVQLEGAFPDTMTKCAELLNRTID 440
Query: 458 IDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNR 517
+DF+DIN+GCPID+V KG G AL+ + + + +V +G++D P+T+K+RTG E +
Sbjct: 441 VDFVDINVGCPIDLVYKKGGGCALMNRSAKFQQIVRGVNGVLDVPLTVKMRTGVQERVSL 500
Query: 518 IDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAD 577
L+ ++ +WG VT+HGR+R+QRY++LADW YI +C+ +A + + GNGD+ S+ D
Sbjct: 501 AHRLLPELRDWGVALVTLHGRSREQRYTRLADWPYIEQCAKVA-SPMPLFGNGDILSFED 560
Query: 578 WNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 637
N +A M+ARGAL+KPWLFTEIKEQR+WDI+S ERL+IL+DF H+GLEHWGS
Sbjct: 561 AN--CAMQTGVAGIMVARGALLKPWLFTEIKEQRHWDISSSERLDILRDFTHYGLEHWGS 620
Query: 638 DTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADW 691
DT+GVE TR FLLEWLS+ CRY+PVGLL+ PQR+N RPP Y GRD LETLM+S AADW
Sbjct: 621 DTQGVERTRRFLLEWLSFLCRYVPVGLLERPPQRINERPPYYLGRDYLETLMASQQAADW 636
BLAST of Carg19376 vs. ExPASy Swiss-Prot
Match:
Q96G46 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS3L PE=1 SV=2)
HSP 1 Score: 518.1 bits (1333), Expect = 1.6e-145
Identity = 279/640 (43.59%), Postives = 377/640 (58.91%), Query Frame = 0
Query: 62 DTASTGKNDGDDKAAPSSLVKEKKSKRQMKRER---RQEQKSTSHLCPEIAKTGDVSSCR 121
+ T ++ A P ++ +K R + R + + LCP + + + C
Sbjct: 78 EDGQTADGQTEEAAEPGEQLQTQKRARGQNKGRPHVKPTNYDKNRLCPSLIQE-SAAKCF 137
Query: 122 YSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALK 181
+ D+CRF HD+ + KPADL C CPYG+ CRFAG H P G ++ +
Sbjct: 138 FGDRCRFLHDVGRYLETKPADLGPRCVLFETFGRCPYGVTCRFAGAHLR--PEGQNLVQE 197
Query: 182 KC--------SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDV 241
+ S NGL+K +Q+ L K +++F +A+ L+ G + +
Sbjct: 198 ELAARGTQPPSIRNGLDKALQQQLRKREVRFERAEQALRR---FSQGPTPA--------- 257
Query: 242 VSNDSAADRTRCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCH 301
+P EGT + R ENC
Sbjct: 258 --------------------------AAVP------EGTAAEGAPR---------QENCG 317
Query: 302 SDKIDNGACMIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPL 361
+ ++ G TP S V L D D ++ P EKK +D R KLYLAPL
Sbjct: 318 AQQVPAG--------PGTSTPPSSPVRTCGPLTDEDV-VRLRPCEKKRLDIRGKLYLAPL 377
Query: 362 TTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPD 421
TT GNLPFRR+CK GADVTCGEMA+CTNLLQGQ SEWALL+RH ED+FGVQ+ GA+PD
Sbjct: 378 TTCGNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHQCEDIFGVQLEGAFPD 437
Query: 422 TVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPI 481
T+++ EL+ + +DF+DIN+GCPID+V KG G AL+ + + + +V + ++D P+
Sbjct: 438 TMTKCAELLSRTVEVDFVDINVGCPIDLVYKKGGGCALMNRSTKFQQIVRGMNQVLDVPL 497
Query: 482 TIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDS 541
T+K+RTG E N L+ ++ +WG VT+HGR+R+QRY+KLADW+YI C A
Sbjct: 498 TVKIRTGVQERVNLAHRLLPELRDWGVALVTLHGRSREQRYTKLADWQYIEECVQ-AASP 557
Query: 542 LQVIGNGDVFSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNI 601
+ + GNGD+ S+ D NR + MIARGAL+KPWLFTEIKEQR+WDI+S ERL+I
Sbjct: 558 MPLFGNGDILSFEDANR--AMQTGVTGIMIARGALLKPWLFTEIKEQRHWDISSSERLDI 617
Query: 602 LKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRD 661
L+DF ++GLEHWGSDT+GVE TR FLLEWLS+ CRY+PVGLL+ +PQR+N RPP Y GRD
Sbjct: 618 LRDFTNYGLEHWGSDTQGVEKTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLGRD 649
Query: 662 DLETLMSSDSAADWIKISEMLLGKVPDAFSFAPKHKSNAY 691
LETLM+S AADWI+ISEMLLG VP +F+F PKHK+NAY
Sbjct: 678 YLETLMASQKAADWIRISEMLLGPVPPSFAFLPKHKANAY 649
BLAST of Carg19376 vs. ExPASy Swiss-Prot
Match:
Q7ZWS1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Xenopus laevis OX=8355 GN=dus3l PE=2 SV=1)
HSP 1 Score: 514.6 bits (1324), Expect = 1.7e-144
Identity = 279/638 (43.73%), Postives = 376/638 (58.93%), Query Frame = 0
Query: 67 GKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKST----SHLCPEIAKTGDVSSCRYSDK 126
G ++G D+ ++ K++K + + + + R K + + LCP + + S C + DK
Sbjct: 87 GSSEGQDQPPKTAENKQEKKRARGQNKSRPHMKHSQFEENKLCPSVTQE-CASKCFFGDK 146
Query: 127 CRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTH----------KEGVPAG 186
C+FSHD+ + SQKP D+ C C YG+ CRFA +H +E +
Sbjct: 147 CKFSHDVAKYVSQKPEDIRPNCHLYETFGKCIYGVTCRFAKSHLGEDFKNIINEELMKEW 206
Query: 187 SSVALKKCSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVS 246
L K N L+K +++ L K K+ F K+D LK G + K VV
Sbjct: 207 EGKVLVK----NSLDKSLKEQLRKKKVVFEKSDKYLKLCFKSGDS------SKMKNPVVK 266
Query: 247 NDSAADRTRCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSD 306
DSA T+
Sbjct: 267 EDSAVQVTQ--------------------------------------------------- 326
Query: 307 KIDNGACMIDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTT 366
DS + + D D +K P EKK IDFR+KLYLAPLTT
Sbjct: 327 -------------------KDSPITTVGAVTDEDV-IKLRPCEKKTIDFRNKLYLAPLTT 386
Query: 367 VGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTV 426
GNLPFRR+CK LGAD+TCGEMAMCTNLLQGQ SEWALL+RH SED+FGVQ+ GA+PDT+
Sbjct: 387 CGNLPFRRICKRLGADITCGEMAMCTNLLQGQPSEWALLKRHHSEDIFGVQLEGAFPDTM 446
Query: 427 SRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITI 486
++ EL+++ +DF+DIN+GCPID+V KG G L+ + + + +V+ + ++D P+T+
Sbjct: 447 TKCAELLNRTIDVDFVDINVGCPIDLVYKKGGGCGLMNRTNKFEQIVKGMNSVLDVPLTV 506
Query: 487 KVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQ 546
K+RTG E N LI ++ +WG VT+HGR+R+QRY+KLA+WEYI++C+ +A +
Sbjct: 507 KIRTGVQEKVNIAHKLIPNLRDWGVSLVTLHGRSREQRYTKLANWEYIDQCAKIA-SPVP 566
Query: 547 VIGNGDVFSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILK 606
+ GNGD+ SY D NR +A M+ARGAL KPWLFTEIKEQR+WDI+S ER +ILK
Sbjct: 567 LFGNGDIISYEDANR--ALQTGVAGVMLARGALFKPWLFTEIKEQRHWDISSTERFDILK 626
Query: 607 DFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDL 666
DF ++GLEHWGSD +GVE TR F+LEWLS+ CRYIPVGLL+ VPQ++N RPP Y GRD L
Sbjct: 627 DFTNYGLEHWGSDCQGVEKTRKFMLEWLSFLCRYIPVGLLEHVPQKINERPPYYMGRDYL 639
Query: 667 ETLMSSDSAADWIKISEMLLGKVPDAFSFAPKHKSNAY 691
ETLM+S + DWIKISEMLLG VP F+F PKHK+N+Y
Sbjct: 687 ETLMASQNVTDWIKISEMLLGPVPPNFTFLPKHKANSY 639
BLAST of Carg19376 vs. ExPASy TrEMBL
Match:
A0A6J1GL24 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita moschata OX=3662 GN=LOC111455351 PE=3 SV=1)
HSP 1 Score: 1399.0 bits (3620), Expect = 0.0e+00
Identity = 693/696 (99.57%), Postives = 695/696 (99.86%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPP+RSSCNNV
Sbjct: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPMRSSCNNV 60
Query: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM
Sbjct: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
SYADWNRHKVESPKLATC IARGALIKPWLFTEIKEQRNWDITSGERLNILK+FVHFGLE
Sbjct: 541 SYADWNRHKVESPKLATCTIARGALIKPWLFTEIKEQRNWDITSGERLNILKNFVHFGLE 600
Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 696
BLAST of Carg19376 vs. ExPASy TrEMBL
Match:
A0A6J1HXQ8 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita maxima OX=3661 GN=LOC111468431 PE=3 SV=1)
HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 681/696 (97.84%), Postives = 687/696 (98.71%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV
Sbjct: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNV 60
Query: 61 KDTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
KD ASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS
Sbjct: 61 KDAASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYS 120
Query: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKC 180
DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LKKC
Sbjct: 121 DKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKKC 180
Query: 181 SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDSAADRT 240
SEMNGLNKDVQKLLWKNKMKFPKAD+KLKELGLLGHGKSKSRVKEEKED VSNDS ADRT
Sbjct: 181 SEMNGLNKDVQKLLWKNKMKFPKADAKLKELGLLGHGKSKSRVKEEKEDAVSNDSVADRT 240
Query: 241 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 300
C EVNNDMDADLDIP+E+PEENGQTEGTFSSDESRPLKKPKSMSSENCHSD+IDNGACM
Sbjct: 241 CCSEVNNDMDADLDIPLEVPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDEIDNGACM 300
Query: 301 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
IDATI EGHTPI+SEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 301 IDATIREGHTPIESEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 360
Query: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 361 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 420
Query: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 480
KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE ASGIVDTPITIKVRTGYFE
Sbjct: 421 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVEIASGIVDTPITIKVRTGYFE 480
Query: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 481 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 540
Query: 541 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
SYA+WNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE
Sbjct: 541 SYAEWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 600
Query: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 601 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 660
Query: 661 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
AADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 661 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 696
BLAST of Carg19376 vs. ExPASy TrEMBL
Match:
A0A0A0K4I1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7G007900 PE=3 SV=1)
HSP 1 Score: 1271.5 bits (3289), Expect = 0.0e+00
Identity = 624/696 (89.66%), Postives = 655/696 (94.11%), Query Frame = 0
Query: 2 EDSVSAELRDPTAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVK 61
EDSV+A+L D TAVSE P S S+ G LTP++LVAKAIAPVKR++LRPPP+RSS NN+K
Sbjct: 3 EDSVTADLLDDTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSSNNLK 62
Query: 62 DTASTGKNDGDDKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSD 121
D ST KNDGDDKAAPS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDVSSCRYSD
Sbjct: 63 DATSTAKNDGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSD 122
Query: 122 KCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCS 181
KCRFSHDIEAFKSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LK+CS
Sbjct: 123 KCRFSHDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCS 182
Query: 182 EMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDS-AADRT 241
EMNGLNKD+QKLLWKNKMKFPKADSKLKELGLLGHGKSK RVKE++E+ VSNDS ADR
Sbjct: 183 EMNGLNKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRP 242
Query: 242 RCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACM 301
C E+NND+DADL+I VE+PEENG+ EGTFSSDESRPLKK KS+SSEN HSD+IDNGACM
Sbjct: 243 CCSEMNNDLDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDNGACM 302
Query: 302 IDATISEGHTPIDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRR 361
ID TI EGH PI+SE +NDSVL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 303 IDETIGEGHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRR 362
Query: 362 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 421
VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID
Sbjct: 363 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 422
Query: 422 KECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFE 481
KECV+DFIDINMGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+E
Sbjct: 423 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYE 482
Query: 482 GKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 541
GKNRIDSLIADIGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF
Sbjct: 483 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVF 542
Query: 542 SYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 601
SY DWN HK E PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGLE
Sbjct: 543 SYTDWNEHKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLE 602
Query: 602 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDS 661
HWGSD+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDS
Sbjct: 603 HWGSDSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDS 662
Query: 662 AADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
AADWIKISEMLLGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 663 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698
BLAST of Carg19376 vs. ExPASy TrEMBL
Match:
A0A5D3CK47 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G001380 PE=3 SV=1)
HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 615/685 (89.78%), Postives = 643/685 (93.87%), Query Frame = 0
Query: 13 TAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDTASTGKNDGD 72
TAVSE P S SA G TP++LVAKAIAPV ++LRPPP+RSS N +KD ST NDGD
Sbjct: 7 TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNTLKDATSTANNDGD 66
Query: 73 DKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAF 132
DKA PS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDV+SCRYSDKCRFSHDIEAF
Sbjct: 67 DKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFSHDIEAF 126
Query: 133 KSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCSEMNGLNKDVQK 192
KSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LK+CSEMNGLNKD+QK
Sbjct: 127 KSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQK 186
Query: 193 LLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDS-AADRTRCGEVNNDMDA 252
LLWKNKMKFPKADSKLKELGLLGHGKSK RVKE+KE+ VSNDS ADRT C E NND+DA
Sbjct: 187 LLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSETNNDLDA 246
Query: 253 DLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACMIDATISEGHTP 312
DL+IPVE+PEENG+ EGTFSSDESRPLKK KS+SS N HSD+IDNGACMID TI EGH P
Sbjct: 247 DLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGACMIDETIGEGHAP 306
Query: 313 IDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC 372
I+SEV+ND VL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC
Sbjct: 307 IESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC 366
Query: 373 GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDIN 432
GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV+DFIDIN
Sbjct: 367 GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDIN 426
Query: 433 MGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNRIDSLIAD 492
MGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNRIDSLIAD
Sbjct: 427 MGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIAD 486
Query: 493 IGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNRHKVE 552
IGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWN+HK E
Sbjct: 487 IGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNQHKDE 546
Query: 553 SPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVET 612
PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSD+KGVET
Sbjct: 547 CPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDSKGVET 606
Query: 613 TRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML 672
TRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML
Sbjct: 607 TRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML 666
Query: 673 LGKVPDAFSFAPKHKSNAYDRAENG 697
LGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 667 LGKVPDGFSFAPKHKSNAYDRAENG 691
BLAST of Carg19376 vs. ExPASy TrEMBL
Match:
A0A1S3BIS3 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo OX=3656 GN=LOC103490332 PE=3 SV=1)
HSP 1 Score: 1255.0 bits (3246), Expect = 0.0e+00
Identity = 615/685 (89.78%), Postives = 643/685 (93.87%), Query Frame = 0
Query: 13 TAVSEEPASLSASTGAQLTPEELVAKAIAPVKREYLRPPPVRSSCNNVKDTASTGKNDGD 72
TAVSE P S SA G TP++LVAKAIAPV ++LRPPP+RSS N +KD ST NDGD
Sbjct: 7 TAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSSNTLKDATSTANNDGD 66
Query: 73 DKAAPSSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDVSSCRYSDKCRFSHDIEAF 132
DKA PS+LVKEKKSKRQ+KRERRQEQKST HLCPEIAKTGDV+SCRYSDKCRFSHDIEAF
Sbjct: 67 DKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFSHDIEAF 126
Query: 133 KSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVALKKCSEMNGLNKDVQK 192
KSQKPADL+GECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSV LK+CSEMNGLNKD+QK
Sbjct: 127 KSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKDIQK 186
Query: 193 LLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSNDS-AADRTRCGEVNNDMDA 252
LLWKNKMKFPKADSKLKELGLLGHGKSK RVKE+KE+ VSNDS ADRT C E NND+DA
Sbjct: 187 LLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSETNNDLDA 246
Query: 253 DLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSSENCHSDKIDNGACMIDATISEGHTP 312
DL+IPVE+PEENG+ EGTFSSDESRPLKK KS+SS N HSD+IDNGACMID TI EGH P
Sbjct: 247 DLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGACMIDETIGEGHAP 306
Query: 313 IDSEVVNDSVLPDSDASLKSHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC 372
I+SEV+ND VL DSDASLK HPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC
Sbjct: 307 IESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTC 366
Query: 373 GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVIDFIDIN 432
GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV+DFIDIN
Sbjct: 367 GEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDIN 426
Query: 433 MGCPIDIVVNKGAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNRIDSLIAD 492
MGCPIDIVVNKGAGSALL KPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNRIDSLIAD
Sbjct: 427 MGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDSLIAD 486
Query: 493 IGNWGAGAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNRHKVE 552
IGNWGA AVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWN+HK E
Sbjct: 487 IGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNQHKDE 546
Query: 553 SPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVET 612
PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSD+KGVET
Sbjct: 547 CPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDSKGVET 606
Query: 613 TRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML 672
TRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML
Sbjct: 607 TRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKISEML 666
Query: 673 LGKVPDAFSFAPKHKSNAYDRAENG 697
LGKVPD FSFAPKHKSNAYDRAENG
Sbjct: 667 LGKVPDGFSFAPKHKSNAYDRAENG 691
BLAST of Carg19376 vs. TAIR 10
Match:
AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )
HSP 1 Score: 869.8 bits (2246), Expect = 1.5e-252
Identity = 447/716 (62.43%), Postives = 544/716 (75.98%), Query Frame = 0
Query: 1 MEDSVSAELRDPTAVSEEPASLSASTGAQL-----TPEELVAKAIAPVKREYLRPPPVRS 60
M D ++ D + + A+L + Q T EEL+ +++AP+K+E+L PPP
Sbjct: 1 MSDVIAEHAVDTSVEGDAKAALPVQSTKQFSVYEATSEELIERSMAPIKKEFLCPPP--- 60
Query: 61 SCNNVKDTASTGKNDGDDKAAP-SSLVKEKKSKRQMKRERRQEQKSTSHLCPEIAKTGDV 120
+ S +ND D AP S LV+EKKSKRQ+KRERR++ ST +LCP++A+T DV
Sbjct: 61 ------PSRSVKQNDAADVRAPQSGLVQEKKSKRQLKRERREQ--STINLCPQVARTEDV 120
Query: 121 SSCRYSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSS 180
SC+Y DKCRF+HDIEAFK+QK D++G+CPF + C YGL+CRF G+H++ G+S
Sbjct: 121 DSCQYKDKCRFNHDIEAFKAQKADDIEGQCPFVASGMKCAYGLSCRFLGSHRD--ITGNS 180
Query: 181 VALKKCSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLGHGKSKSRVKEEKEDVVSN- 240
+K SEMN NK+ Q+LLWKNKM F AD+KLK LGLLGH K + +E + N
Sbjct: 181 DDKEK-SEMNFFNKETQRLLWKNKMTFTNADAKLKSLGLLGHAKKSNAAEEITAEKTQNG 240
Query: 241 -------DSAADRTRCGEVNNDMDADLDIPVEIPEENGQTEGTFSSDESRPLKKPKSMSS 300
+ A D E ++M D+DIP G ++E RP+KK KS
Sbjct: 241 MNGTQATEVAVDSAVSSEHTSEMIQDVDIP-----------GPLETEEVRPMKKAKSEDQ 300
Query: 301 ENCHSDKID---NGACMIDATISEGHTPIDSEVVN-DSV-LPDSDASLKSHPRE-KKLID 360
+N + + +G + + T G+ + V + DS+ + ++D+SLK HPRE KKLID
Sbjct: 301 KNSKTGDVGGVYDGVKLEEETKKNGYPTSKANVEDEDSIKIVETDSSLKLHPREKKKLID 360
Query: 361 FRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
FRDKLYLAPLTTVGNLPFRR+CKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF
Sbjct: 361 FRDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLF 420
Query: 421 GVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSVVE 480
GVQICG+YPDTVSR VELID+EC +DFIDINMGCPID+VVNK AGSALL KP+RMK++VE
Sbjct: 421 GVQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVE 480
Query: 481 TASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSKLADWEYI 540
+S IV+TPITIKVRT +FEGKNRIDSLIADIGNWGA AVTIHGR+RQQRYSK ADW+YI
Sbjct: 481 VSSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKSADWDYI 540
Query: 541 NRCSSLAPDSLQVIGNGDVFSYADWNRHKVESPKLATCMIARGALIKPWLFTEIKEQRNW 600
+C+ A +LQVIGNGDV+SY DWN+HK + P+L++CMIARGALIKPW+FTEIKEQR+W
Sbjct: 541 YQCTKNATTNLQVIGNGDVYSYLDWNKHKSDCPELSSCMIARGALIKPWIFTEIKEQRHW 600
Query: 601 DITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLN 660
DITSGERLNI+KDFV FGL+HWGSDTKGVETTRHFLLEWLSYT RYIPVGLLDV+PQ++N
Sbjct: 601 DITSGERLNIMKDFVRFGLQHWGSDTKGVETTRHFLLEWLSYTFRYIPVGLLDVIPQQIN 660
Query: 661 WRPPSYFGRDDLETLMSSDSAADWIKISEMLLGKVPDAFSFAPKHKSNAYDRAENG 697
WRPPSYFGRDDLETLM S+SA DW++ISEMLLGKVP+ F+FAPKHKSNAYDRAENG
Sbjct: 661 WRPPSYFGRDDLETLMMSESAGDWVRISEMLLGKVPEGFTFAPKHKSNAYDRAENG 691
BLAST of Carg19376 vs. TAIR 10
Match:
AT3G49640.1 (Aldolase-type TIM barrel family protein )
HSP 1 Score: 110.9 bits (276), Expect = 4.1e-24
Identity = 87/309 (28.16%), Postives = 145/309 (46.93%), Query Frame = 0
Query: 339 IDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGE------MAMCTNLLQGQASEWALLR 398
+D+++KL LAP+ VG L FR + GAD+T GE + C L + +
Sbjct: 1 MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60
Query: 399 RHSSEDLFGVQICGAYPDTV---------SRAVELIDKEC-VIDFIDINMGCPIDIVVNK 458
+ + +F C V RA++ + C + IDINMGCP +
Sbjct: 61 KGTDNVVFST--CDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQG 120
Query: 459 GAGSALLMKPMRMKSVVETASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGAGAVTI 518
G G+ALL KP + ++ T +D P+T K+R + L I G A+ +
Sbjct: 121 GMGAALLSKPELIHDILATLKRNLDVPVTCKIR--LLKSPADTVELARRIEKLGVPALAV 180
Query: 519 HGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYADWNRHKVESPKLATCMIAR 578
HGR R A W+ I +A S+ VI NGDV Y D++R K + A+ M+AR
Sbjct: 181 HGRKIADRPRDPAKWDEI--ADVVAALSIPVIANGDVLEYDDFSRIKT-ATGAASVMVAR 240
Query: 579 GALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLS- 631
GA+ +F+ K + +W+ ++ K ++ + W +D V++T++ + E ++
Sbjct: 241 GAMWNASIFSP-KGKSHWE-------DVKKKYLRKSI-LWNND---VKSTKYTIKEMIAH 290
BLAST of Carg19376 vs. TAIR 10
Match:
AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )
HSP 1 Score: 77.4 bits (189), Expect = 5.0e-14
Identity = 58/204 (28.43%), Postives = 95/204 (46.57%), Query Frame = 0
Query: 342 RDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEM---AMCTNLLQGQASEWALLRRHSSED 401
R K +AP+ LPFR +C+ GA M + T + + E+ +
Sbjct: 87 RPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQEFTTCK--EDRP 146
Query: 402 LFGVQICGAYPDTVSRAVELIDKECVIDFIDINMGCPIDIVVNKGAGSALLMKPMRMKSV 461
LF VQ C PDT+ A + ++ C D++DIN+GCP I G+ L+ +KS+
Sbjct: 147 LF-VQFCANDPDTLLEAAKRVEPYC--DYVDINLGCPQRIARRGNYGAFLMDNLPLVKSL 206
Query: 462 VETASGIVDTPITIKVRT-GYFEGKNRIDSLIADIGNWGAGAVTIHGRTRQQRYSK--LA 521
VE + ++ P++ K+R E + ++ D G + +HGRTR ++ K A
Sbjct: 207 VEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDA---GCSLLAVHGRTRDEKDGKKFRA 266
Query: 522 DWEYINRCSSLAPDSLQVIGNGDV 540
DW I + + V+ NG+V
Sbjct: 267 DWSAIKEVKNAM--RIPVLANGNV 280
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG6572077.1 | 0.0e+00 | 100.00 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like protein, partial [Cucurbita ar... | [more] |
XP_022952746.1 | 0.0e+00 | 99.57 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita moschata] | [more] |
XP_022969416.1 | 0.0e+00 | 97.84 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita maxima] | [more] |
XP_023554076.1 | 0.0e+00 | 97.70 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo] | [more] |
XP_004148548.1 | 0.0e+00 | 89.66 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 ... | [more] |
Match Name | E-value | Identity | Description | |
Q9T0J6 | 2.1e-251 | 62.43 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=370... | [more] |
Q7XT07 | 1.7e-245 | 63.34 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonic... | [more] |
Q91XI1 | 9.2e-146 | 41.98 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Du... | [more] |
Q96G46 | 1.6e-145 | 43.59 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS... | [more] |
Q7ZWS1 | 1.7e-144 | 43.73 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Xenopus laevis OX=8355 GN=d... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1GL24 | 0.0e+00 | 99.57 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita moschata OX=3662 GN=LO... | [more] |
A0A6J1HXQ8 | 0.0e+00 | 97.84 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucurbita maxima OX=3661 GN=LOC1... | [more] |
A0A0A0K4I1 | 0.0e+00 | 89.66 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7... | [more] |
A0A5D3CK47 | 0.0e+00 | 89.78 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194... | [more] |
A0A1S3BIS3 | 0.0e+00 | 89.78 | tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo OX=3656 GN=LOC10349... | [more] |
Match Name | E-value | Identity | Description | |
AT4G38890.1 | 1.5e-252 | 62.43 | FMN-linked oxidoreductases superfamily protein | [more] |
AT3G49640.1 | 4.1e-24 | 28.16 | Aldolase-type TIM barrel family protein | [more] |
AT5G67220.1 | 5.0e-14 | 28.43 | FMN-linked oxidoreductases superfamily protein | [more] |