Carg17964 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17964
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein EMBRYO DEFECTIVE 514-like
Location	Carg_Chr10: 878468 .. 880836 (+)
RNA-Seq Expression	Carg17964
Synteny	Carg17964

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAGAAGAAACAATCCCTCAACCGGCTGACAACGATCAGGAGGTGACCAACGCCGAAGACATGGAATTGGAGAACACAGAGCCCGGAGCAGAACCGAAAGGATCAAATGATGAAGCAAATGAGGCTGCAAAAGTAACTAATGGCGATGCAAATTCGAAGCGGGAGAGGGAGGAAGAAAGCGCCGATGGCAGCGTCGAAGAGGTGAAAAAGCAGAAGATGGAGAAGTCAGTTGAAGAGGGAAGGGTTGAGAAGGCGCAAGGAGACGGGAAGGGAGAGGAGGAGCCTGGTCCTGTGACTATAGGCCCTAAGAGCTTTCGATCGTCGAGGGAATTGTTTGATCACTTCTATAAGTTCCTCCACCACTGGCCTGCCTACTTGAACGTGAACCAGGTTTCATATTATGGACTTCTTTTTAATTTGCTATCGTCCTCTGTTTTGTTAATTGAAAGGTTAAGTTGAGAATGATGCTTATGAAATTTTATGTTAGTTTTAGAACGCAGAGCTGGAACTTGATTGACATATTTTCTTTTTTCCCAATATTGCTGTTATGGTTTATCATCATCATTATTTTAATTTGTGAAAGTTACATCTTTCTGAAGGGAAAACGAATTGCAAAAGGAGGTGTAGATTTAGGTATCTTGATCCTCATGAATGGTGAACAATTTTAGCAGGACTTTTTGTTCTAGTCACTTGTTTGAGAAACTGCTATAATTGTGTACATAATAGAATCAGAGAGGTGACATTCTTCAATATTCTCATGAGATTAATGATGATTGGTATCAGTTTTGCCCAAAATCCATTTACAGATTTAGATGTTAAAACAATTAATCAAAGAATCCCACCCTGGATGAATAAATTTATTTCTATCTGTGAATCTACTGTCTTGACCTTATTTTCTCATTGAAGAATTTCTTTTAGCTGTTTTCTTTTTTTTTTCTCTTTTTTTTTTTTTTTGCATTGTTTTGCTGTTATTCTTTATGATTCCCTTCTTATTCTTGATTGATGTTAGTGGAAGAAATAGATATGCTATATCTATTTATTGCTTTCTTTCTAGCATATCCTGTGATGTAGCAAATTATACATACAAGTATGGCTTAACTCTTCAAAACCAAGAGCCGCACCAAGCCATTGAGCATGTCCTTATATGGTATAATTTTGCAGCCCAAAAGGGGAAGTAATAATTAGTGTTTAGAAATCCATGTATTCATGGCCGGTTACAGGGTGTGATCGCTTAGTTATGAAAAAAATCCTTAAATTGAAAGACAGTTGGGTGTTATTATTTGTATTCAAATATTATTTCTACTAAAGTCTATTCCTTTAGCACTTAGTGTAACAGCCCAAACCAACCACCGCTAGCCCATATTGTCCTCTTTAGATTTTTCTTCAAGGTTTTTACGACGCATGTACTAGGCAGAGATTTCCACACCCTTATAAGGAATGTGTTACTCCTCTTTTCAACTGATGTGAGATCTCACAATCCCACCTCCCTTAGAGCCCGGCGTCCTCGTTGGCACACCATCCGATGTCTGGCTTTGATACCATTTGTAACCGCCCAAGCCCACCACTACAAATATTGTCCTCTTTGGACTTTCTCTTTTGGGCTTCCCTTCTAGGTTTTAAAATGTGTCTACTAGGAAGAGGTTTCCACACTCTTATAAGGAATGTTTCGTTTTTCTTTCCAACTGACGTGGGACCTCACATTTAGCCTACTTTACTATAACTTGGTTAAACATTAGTAATATCTTCTGTAGGTTTGTATTCTTGTTCTGTGTTTGAATAGTAATATTTAAAAGAAAAAGAGTGTATTTCTTTAGGGGCCCAGTATTATCTTGTCGGTCATGTTTTTCGTCTAGTCAACCGCCATTCTAAGGTGAAAAACTCTACTAATTTTACCCCCTAAGTCATTGAAAACATCTATAATTGATCAAATTTACACGATGAATAATAAATTGATTCATGGTAAAACTATTTGCATATTGTGGAGGCATTGATTCTGTTGTTGATTCAATATTTGATATCGGTCTGTGTGGCAGTACGAGAAGATGATGTTGCTGGATTTGCTGAAGAAGGGGCATGCAGAACCAGAGAAAAAGATAGGGTGCGGTATTCATTCTTTCCAAATTCGAAACCATCCTATTTGGAAAAACAAGTGCTTCTTCCTCATTCGGGAGGACGAGTCTGTTGATGACTTCAGCTTCAGAAAGTGTGTGGATCATATCCTTCCCCTTCCTGAAAACCTCAAAGGTAGATCTGATGCAAACAAGGCATTCGGTGGTGGCAAGCACCGCGGTGGCGGTGGAGGACGAGGCGGGGGTGGTGGTGGCCGTGGAAGAGGAGGAGGAGGAGGGGGTAGGTGGAGAAACTGA

mRNA sequence

ATGGCAGAAGAAACAATCCCTCAACCGGCTGACAACGATCAGGAGGTGACCAACGCCGAAGACATGGAATTGGAGAACACAGAGCCCGGAGCAGAACCGAAAGGATCAAATGATGAAGCAAATGAGGCTGCAAAAGTAACTAATGGCGATGCAAATTCGAAGCGGGAGAGGGAGGAAGAAAGCGCCGATGGCAGCGTCGAAGAGGTGAAAAAGCAGAAGATGGAGAAGTCAGTTGAAGAGGGAAGGGTTGAGAAGGCGCAAGGAGACGGGAAGGGAGAGGAGGAGCCTGGTCCTGTGACTATAGGCCCTAAGAGCTTTCGATCGTCGAGGGAATTGTTTGATCACTTCTATAAGTTCCTCCACCACTGGCCTGCCTACTTGAACGTGAACCAGTACGAGAAGATGATGTTGCTGGATTTGCTGAAGAAGGGGCATGCAGAACCAGAGAAAAAGATAGGGTGCGGTATTCATTCTTTCCAAATTCGAAACCATCCTATTTGGAAAAACAAGTGCTTCTTCCTCATTCGGGAGGACGAGTCTGTTGATGACTTCAGCTTCAGAAAGTGTGTGGATCATATCCTTCCCCTTCCTGAAAACCTCAAAGGTAGATCTGATGCAAACAAGGCATTCGGTGGTGGCAAGCACCGCGGTGGCGGTGGAGGACGAGGCGGGGGTGGTGGTGGCCGTGGAAGAGGAGGAGGAGGAGGGGGTAGGTGGAGAAACTGA

Coding sequence (CDS)

ATGGCAGAAGAAACAATCCCTCAACCGGCTGACAACGATCAGGAGGTGACCAACGCCGAAGACATGGAATTGGAGAACACAGAGCCCGGAGCAGAACCGAAAGGATCAAATGATGAAGCAAATGAGGCTGCAAAAGTAACTAATGGCGATGCAAATTCGAAGCGGGAGAGGGAGGAAGAAAGCGCCGATGGCAGCGTCGAAGAGGTGAAAAAGCAGAAGATGGAGAAGTCAGTTGAAGAGGGAAGGGTTGAGAAGGCGCAAGGAGACGGGAAGGGAGAGGAGGAGCCTGGTCCTGTGACTATAGGCCCTAAGAGCTTTCGATCGTCGAGGGAATTGTTTGATCACTTCTATAAGTTCCTCCACCACTGGCCTGCCTACTTGAACGTGAACCAGTACGAGAAGATGATGTTGCTGGATTTGCTGAAGAAGGGGCATGCAGAACCAGAGAAAAAGATAGGGTGCGGTATTCATTCTTTCCAAATTCGAAACCATCCTATTTGGAAAAACAAGTGCTTCTTCCTCATTCGGGAGGACGAGTCTGTTGATGACTTCAGCTTCAGAAAGTGTGTGGATCATATCCTTCCCCTTCCTGAAAACCTCAAAGGTAGATCTGATGCAAACAAGGCATTCGGTGGTGGCAAGCACCGCGGTGGCGGTGGAGGACGAGGCGGGGGTGGTGGTGGCCGTGGAAGAGGAGGAGGAGGAGGGGGTAGGTGGAGAAACTGA

Protein sequence

MAEETIPQPADNDQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEESADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFLHHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDESVDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGGGRWRN

Homology

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BLAST of Carg17964 vs. NCBI nr
Match: KAG7023193.1 (Protein EMBRYO DEFECTIVE, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 454.5 bits (1168), Expect = 5.6e-124
Identity = 241/241 (100.00%), Postives = 241/241 (100.00%), Query Frame = 0


Query: 1   MAEETIPQPADNDQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEE 60
           MAEETIPQPADNDQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEE
Sbjct: 1   MAEETIPQPADNDQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEE 60

Query: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120
           SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL
Sbjct: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120

Query: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180
           HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES
Sbjct: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180

Query: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGGGRWR 240
           VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGGGRWR
Sbjct: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGGGRWR 240

Query: 241 N 242
           N
Sbjct: 241 N 241

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BLAST of Carg17964 vs. NCBI nr
Match: XP_022921344.1 (protein EMBRYO DEFECTIVE 514-like [Cucurbita moschata])

HSP 1 Score: 442.6 bits (1137), Expect = 2.2e-120
Identity = 237/241 (98.34%), Postives = 239/241 (99.17%), Query Frame = 0


Query: 1   MAEETIPQPADNDQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEE 60
           MAEETIPQPA+N+QEVTNAEDMELENTEP AEPKGSNDEANEAAKVTNGDANSKREREEE
Sbjct: 1   MAEETIPQPAENNQEVTNAEDMELENTEPVAEPKGSNDEANEAAKVTNGDANSKREREEE 60

Query: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120
           SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL
Sbjct: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120

Query: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180
           HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES
Sbjct: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180

Query: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGGGRWR 240
           VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGR GGGGGRWR
Sbjct: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGR-GGGGGRWR 240

Query: 241 N 242
           N
Sbjct: 241 N 240

[Close]

BLAST of Carg17964 vs. NCBI nr
Match: KAG6589507.1 (Protein EMBRYO DEFECTIVE 514, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 441.0 bits (1133), Expect = 6.4e-120
Identity = 235/236 (99.58%), Postives = 235/236 (99.58%), Query Frame = 0


Query: 1   MAEETIPQPADNDQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEE 60
           MAEETIPQPADNDQEVTNAEDMELENTEPGAE KGSNDEANEAAKVTNGDANSKREREEE
Sbjct: 1   MAEETIPQPADNDQEVTNAEDMELENTEPGAESKGSNDEANEAAKVTNGDANSKREREEE 60

Query: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120
           SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL
Sbjct: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120

Query: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180
           HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES
Sbjct: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180

Query: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGG 237
           VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGG
Sbjct: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGG 236

[Close]

BLAST of Carg17964 vs. NCBI nr
Match: XP_023515551.1 (protein EMBRYO DEFECTIVE 514-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 439.5 bits (1129), Expect = 1.9e-119
Identity = 236/243 (97.12%), Postives = 239/243 (98.35%), Query Frame = 0


Query: 1   MAEETIPQPADNDQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEE 60
           MAEETIPQPADN+QEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKRER+EE
Sbjct: 1   MAEETIPQPADNNQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKRERDEE 60

Query: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120
           SADGSVEE KKQKMEKSVEEGR+EKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL
Sbjct: 61  SADGSVEEAKKQKMEKSVEEGRIEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120

Query: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180
           HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES
Sbjct: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180

Query: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRG--GGGGRGGGGGGRGRGGGGGGR 240
           VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRG  GGGGRGGGGGGRGR GGGGGR
Sbjct: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGSGGGGRGGGGGGRGR-GGGGGR 240

Query: 241 WRN 242
           WRN
Sbjct: 241 WRN 242

[Close]

BLAST of Carg17964 vs. NCBI nr
Match: XP_022987201.1 (protein EMBRYO DEFECTIVE 514-like [Cucurbita maxima])

HSP 1 Score: 431.4 bits (1108), Expect = 5.1e-117
Identity = 233/244 (95.49%), Postives = 237/244 (97.13%), Query Frame = 0


Query: 1   MAEETIPQPADNDQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEE 60
           MAEETIPQPADN+QEVTNAEDMELENTEPGAEPKGSNDEANEA+K+TNGDANSKREREEE
Sbjct: 1   MAEETIPQPADNNQEVTNAEDMELENTEPGAEPKGSNDEANEASKITNGDANSKREREEE 60

Query: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120
           SADGSVEE KKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL
Sbjct: 61  SADGSVEEAKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120

Query: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180
           HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHP+WKNKCFFLIREDES
Sbjct: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPMWKNKCFFLIREDES 180

Query: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGG---GG 240
           VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGG GG GGGRG GGGG   GG
Sbjct: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGG-GGSGGGRGGGGGGRGRGG 240

Query: 241 RWRN 242
           RWRN
Sbjct: 241 RWRN 243

[Close]

BLAST of Carg17964 vs. ExPASy Swiss-Prot
Match: Q8L557 (Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 4.7e-41
Identity = 115/221 (52.04%), Postives = 140/221 (63.35%), Query Frame = 0


Query: 21  DMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEESADGSVEEVKKQKMEKSVEE 80
           DME+E   P AE                GD   KREREE   + +  E KKQK+ +    
Sbjct: 22  DMEVETATPKAE---------------TGD--EKREREETEEEENGGESKKQKVGE---- 81

Query: 81  GRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFLHHWPAYLNVNQYEKMMLLDL 140
                       EE+ GPV +GPK F +S  +FD+F KFLH WP  L+VN+YE M+LLDL
Sbjct: 82  ------------EEKSGPVKLGPKEFVTSVAMFDYFVKFLHFWPTDLDVNKYEHMVLLDL 141

Query: 141 LKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDESVDDFSFRKCVDHILPLPENL 200
           +KKGH+EPEKKIG GI +FQ+R HP+WK++CFFL+RED++ DDFSFRKCVD ILPLPEN+
Sbjct: 142 IKKGHSEPEKKIGGGIKTFQVRTHPMWKSRCFFLVREDDTADDFSFRKCVDQILPLPENM 201

Query: 201 KGRSDANKAFGGGKHRGGGGGRGGGGGGR-GRGGGGGGRWR 241
           K          G    G GGGRGGGGG R GRGGG GGR+R
Sbjct: 202 K--------TPGANGNGHGGGRGGGGGRRGGRGGGRGGRFR 201

[Close]

BLAST of Carg17964 vs. ExPASy Swiss-Prot
Match: Q9C642 (Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.5e-07
Identity = 27/83 (32.53%), Postives = 49/83 (59.04%), Query Frame = 0


Query: 116 FYKFLHHWPAYLN---VNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWK-NKC 175
           F + + H   Y N   ++   +  ++++L   H E EKKIGCGI    + +HP ++ ++C
Sbjct: 114 FVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRC 173

Query: 176 FFLIREDESVDDFSFRKCVDHIL 195
            F++R+D  V DFS+ KC+  ++
Sbjct: 174 MFIVRKDGEVVDFSYWKCIKGLI 196

[Close]

BLAST of Carg17964 vs. ExPASy TrEMBL
Match: A0A6J1E069 (protein EMBRYO DEFECTIVE 514-like OS=Cucurbita moschata OX=3662 GN=LOC111429642 PE=4 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 1.1e-120
Identity = 237/241 (98.34%), Postives = 239/241 (99.17%), Query Frame = 0


Query: 1   MAEETIPQPADNDQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEE 60
           MAEETIPQPA+N+QEVTNAEDMELENTEP AEPKGSNDEANEAAKVTNGDANSKREREEE
Sbjct: 1   MAEETIPQPAENNQEVTNAEDMELENTEPVAEPKGSNDEANEAAKVTNGDANSKREREEE 60

Query: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120
           SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL
Sbjct: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120

Query: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180
           HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES
Sbjct: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180

Query: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGGGRWR 240
           VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGR GGGGGRWR
Sbjct: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGR-GGGGGRWR 240

Query: 241 N 242
           N
Sbjct: 241 N 240

[Close]

BLAST of Carg17964 vs. ExPASy TrEMBL
Match: A0A6J1JDH2 (protein EMBRYO DEFECTIVE 514-like OS=Cucurbita maxima OX=3661 GN=LOC111484822 PE=4 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 2.5e-117
Identity = 233/244 (95.49%), Postives = 237/244 (97.13%), Query Frame = 0


Query: 1   MAEETIPQPADNDQEVTNAEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEE 60
           MAEETIPQPADN+QEVTNAEDMELENTEPGAEPKGSNDEANEA+K+TNGDANSKREREEE
Sbjct: 1   MAEETIPQPADNNQEVTNAEDMELENTEPGAEPKGSNDEANEASKITNGDANSKREREEE 60

Query: 61  SADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120
           SADGSVEE KKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL
Sbjct: 61  SADGSVEEAKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFL 120

Query: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDES 180
           HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHP+WKNKCFFLIREDES
Sbjct: 121 HHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPMWKNKCFFLIREDES 180

Query: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGG---GG 240
           VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGG GG GGGRG GGGG   GG
Sbjct: 181 VDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGG-GGSGGGRGGGGGGRGRGG 240

Query: 241 RWRN 242
           RWRN
Sbjct: 241 RWRN 243

[Close]

BLAST of Carg17964 vs. ExPASy TrEMBL
Match: A0A6J1C0K4 (protein EMBRYO DEFECTIVE 514 OS=Momordica charantia OX=3673 GN=LOC111007244 PE=4 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 7.2e-85
Identity = 179/237 (75.53%), Postives = 206/237 (86.92%), Query Frame = 0


Query: 1   MAEETIPQPADNDQEVTNA--EDMELENTEPGAEPKGSN--DEANEAAKVTNGDANSKRE 60
           MAEET PQ A+ND EVTN+  EDMELE +EPGAEPKG N   EANEAA  TNGDANSKRE
Sbjct: 1   MAEETTPQLAENDPEVTNSATEDMELETSEPGAEPKGLNGDSEANEAAPETNGDANSKRE 60

Query: 61  REEESADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHF 120
           REEE+AD ++ E KK K+EKSVEE R+EK +GDGKG+E+ GPV++GPKSF SS E+FD+F
Sbjct: 61  REEETADENIGEAKKPKVEKSVEEERMEKVEGDGKGDEKSGPVSLGPKSFGSSVEMFDYF 120

Query: 121 YKFLHHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIR 180
           YKFLH+WPA LN+N+YE+M+LL+LLKKGH EPEKKIGCGIH+FQIRNHP +K+KC+FLIR
Sbjct: 121 YKFLHYWPANLNLNEYEQMVLLELLKKGHVEPEKKIGCGIHAFQIRNHPTFKSKCYFLIR 180

Query: 181 EDESVDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGG 234
           EDESVDDFSFRKCVDHILPLPENLK +SDAN+A GG KHRGGG    GGGGGRGRGG
Sbjct: 181 EDESVDDFSFRKCVDHILPLPENLKVKSDANRALGGCKHRGGG---RGGGGGRGRGG 234

[Close]

BLAST of Carg17964 vs. ExPASy TrEMBL
Match: A0A0A0LRN9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009730 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.4e-80
Identity = 185/243 (76.13%), Postives = 198/243 (81.48%), Query Frame = 0


Query: 1   MAEETIPQPADNDQEVTNA--EDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKRERE 60
           MAEETIPQ    D EVTN+  EDMELE TEP A    S  EANEAA V NGDA SKRER 
Sbjct: 1   MAEETIPQ---LDPEVTNSGTEDMELETTEPKASTDAS--EANEAAPVINGDATSKRER- 60

Query: 61  EESADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYK 120
           EESAD  V + KKQK+EKSVEE R+EK  GDGK EEEP PV++GPKSFRSS ELFD+FYK
Sbjct: 61  EESADDGVADGKKQKIEKSVEEERLEKLGGDGKCEEEPVPVSLGPKSFRSSVELFDYFYK 120

Query: 121 FLHHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIRED 180
            LHHWP  LNVNQYE+M+L+DLLKKGH EPEKKIGCGIHSFQIR HP WK+KCFFLIRED
Sbjct: 121 ILHHWPVNLNVNQYEQMVLVDLLKKGHLEPEKKIGCGIHSFQIRFHPEWKSKCFFLIRED 180

Query: 181 ESVDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGGGR 240
           ES DDFSFRKCVDHILPLPEN KG+ DAN+A GGGKHR  GGGRGGGG   GRG GGGGR
Sbjct: 181 ESADDFSFRKCVDHILPLPENFKGKFDANRALGGGKHR--GGGRGGGG---GRGRGGGGR 232

Query: 241 WRN 242
           WRN
Sbjct: 241 WRN 232

[Close]

BLAST of Carg17964 vs. ExPASy TrEMBL
Match: A0A6J1F569 (protein EMBRYO DEFECTIVE 514-like OS=Cucurbita moschata OX=3662 GN=LOC111440949 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 3.5e-79
Identity = 172/243 (70.78%), Postives = 192/243 (79.01%), Query Frame = 0


Query: 1   MAEETIPQPADNDQEVTN--AEDMELENTEPGAEPKGSNDEANEAAKVTNGDANSKRERE 60
           MAEETIPQ A+N  EVTN  AEDMELE+TEP  +  G + +ANEAA VTNGDANSKRERE
Sbjct: 1   MAEETIPQLAENGPEVTNSAAEDMELESTEP--KVSGGDSDANEAAPVTNGDANSKRERE 60

Query: 61  EESADGSVEEVKKQKMEKSVEEGRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYK 120
           EE AD    E KK K+EKSVEE R+EK + DGKGEEE GPV++GPKSF SS ELFD+FY 
Sbjct: 61  EEGADDHAGEAKKPKVEKSVEEERLEKVERDGKGEEEHGPVSLGPKSFGSSVELFDYFYN 120

Query: 121 FLHHWPAYLNVNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIRED 180
           FLH+WPA LNVNQYE M+LLDL+KKGH EP+KKIGCG+ + QIR HP+WK+KCFFLIRED
Sbjct: 121 FLHYWPANLNVNQYELMVLLDLIKKGHVEPDKKIGCGVRACQIRYHPMWKSKCFFLIRED 180

Query: 181 ESVDDFSFRKCVDHILPLPENLKGRSDANKAFGGGKHRGGGGGRGGGGGGRGRGGGGGGR 240
            S DDFSFRKCVDHILPLPENLK  SDAN+A GGGKHRGG             G GGGGR
Sbjct: 181 GSADDFSFRKCVDHILPLPENLKTISDANRALGGGKHRGG-------------GRGGGGR 228

Query: 241 WRN 242
           WRN
Sbjct: 241 WRN 228

[Close]

BLAST of Carg17964 vs. TAIR 10
Match: AT5G62440.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 169.5 bits (428), Expect = 3.4e-42
Identity = 115/221 (52.04%), Postives = 140/221 (63.35%), Query Frame = 0


Query: 21  DMELENTEPGAEPKGSNDEANEAAKVTNGDANSKREREEESADGSVEEVKKQKMEKSVEE 80
           DME+E   P AE                GD   KREREE   + +  E KKQK+ +    
Sbjct: 22  DMEVETATPKAE---------------TGD--EKREREETEEEENGGESKKQKVGE---- 81

Query: 81  GRVEKAQGDGKGEEEPGPVTIGPKSFRSSRELFDHFYKFLHHWPAYLNVNQYEKMMLLDL 140
                       EE+ GPV +GPK F +S  +FD+F KFLH WP  L+VN+YE M+LLDL
Sbjct: 82  ------------EEKSGPVKLGPKEFVTSVAMFDYFVKFLHFWPTDLDVNKYEHMVLLDL 141

Query: 141 LKKGHAEPEKKIGCGIHSFQIRNHPIWKNKCFFLIREDESVDDFSFRKCVDHILPLPENL 200
           +KKGH+EPEKKIG GI +FQ+R HP+WK++CFFL+RED++ DDFSFRKCVD ILPLPEN+
Sbjct: 142 IKKGHSEPEKKIGGGIKTFQVRTHPMWKSRCFFLVREDDTADDFSFRKCVDQILPLPENM 201

Query: 201 KGRSDANKAFGGGKHRGGGGGRGGGGGGR-GRGGGGGGRWR 241
           K          G    G GGGRGGGGG R GRGGG GGR+R
Sbjct: 202 K--------TPGANGNGHGGGRGGGGGRRGGRGGGRGGRFR 201

[Close]

BLAST of Carg17964 vs. TAIR 10
Match: AT1G45230.1 (Protein of unknown function (DUF3223) )

HSP 1 Score: 56.6 bits (135), Expect = 3.2e-08
Identity = 27/83 (32.53%), Postives = 49/83 (59.04%), Query Frame = 0


Query: 116 FYKFLHHWPAYLN---VNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWK-NKC 175
           F + + H   Y N   ++   +  ++++L   H E EKKIGCGI    + +HP ++ ++C
Sbjct: 114 FVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYIMVGHHPDFESSRC 173

Query: 176 FFLIREDESVDDFSFRKCVDHIL 195
            F++R+D  V DFS+ KC+  ++
Sbjct: 174 MFIVRKDGEVVDFSYWKCIKGLI 196

[Close]

BLAST of Carg17964 vs. TAIR 10
Match: AT1G45230.2 (Protein of unknown function (DUF3223) )

HSP 1 Score: 56.2 bits (134), Expect = 4.1e-08
Identity = 27/83 (32.53%), Postives = 49/83 (59.04%), Query Frame = 0


Query: 116 FYKFLHHWPAYLN---VNQYEKMMLLDLLKKGHAEPEKKIGCGIHSFQIRNHPIWK-NKC 175
           F + + H   Y N   ++   +  ++++L   H E EKKIGCGI    + +HP ++ ++C
Sbjct: 114 FVRMILHSGKYANRDRLSPEHERTIIEMLLPYHPECEKKIGCGIDYIMVWHHPDFESSRC 173

Query: 176 FFLIREDESVDDFSFRKCVDHIL 195
            F++R+D  V DFS+ KC+  ++
Sbjct: 174 MFIVRKDGEVVDFSYWKCIKGLI 196

The following BLAST results are available for this feature:

BLAST of Carg17964 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7023193.1	5.6e-124	100.00	Protein EMBRYO DEFECTIVE, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022921344.1	2.2e-120	98.34	protein EMBRYO DEFECTIVE 514-like [Cucurbita moschata]	[more]
KAG6589507.1	6.4e-120	99.58	Protein EMBRYO DEFECTIVE 514, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023515551.1	1.9e-119	97.12	protein EMBRYO DEFECTIVE 514-like [Cucurbita pepo subsp. pepo]	[more]
XP_022987201.1	5.1e-117	95.49	protein EMBRYO DEFECTIVE 514-like [Cucurbita maxima]	[more]

BLAST of Carg17964 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L557	4.7e-41	52.04	Protein EMBRYO DEFECTIVE 514 OS=Arabidopsis thaliana OX=3702 GN=EMB514 PE=1 SV=1	[more]
Q9C642	4.5e-07	32.53	Protein DCL homolog, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DCL PE=2 S...	[more]

BLAST of Carg17964 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E069	1.1e-120	98.34	protein EMBRYO DEFECTIVE 514-like OS=Cucurbita moschata OX=3662 GN=LOC111429642 ...	[more]
A0A6J1JDH2	2.5e-117	95.49	protein EMBRYO DEFECTIVE 514-like OS=Cucurbita maxima OX=3661 GN=LOC111484822 PE...	[more]
A0A6J1C0K4	7.2e-85	75.53	protein EMBRYO DEFECTIVE 514 OS=Momordica charantia OX=3673 GN=LOC111007244 PE=4...	[more]
A0A0A0LRN9	1.4e-80	76.13	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G009730 PE=4 SV=1	[more]
A0A6J1F569	3.5e-79	70.78	protein EMBRYO DEFECTIVE 514-like OS=Cucurbita moschata OX=3662 GN=LOC111440949 ...	[more]

BLAST of Carg17964 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62440.1	3.4e-42	52.04	Protein of unknown function (DUF3223)	[more]
AT1G45230.1	3.2e-08	32.53	Protein of unknown function (DUF3223)	[more]
AT1G45230.2	4.1e-08	32.53	Protein of unknown function (DUF3223)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF11523	DUF3223	coord: 118..190 e-value: 1.9E-23 score: 82.9
None	No IPR available	GENE3D	3.10.450.40		coord: 95..193 e-value: 2.9E-26 score: 93.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..103
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..92
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 205..241
None	No IPR available	PANTHER	PTHR33415:SF12	PROTEIN EMBRYO DEFECTIVE 514	coord: 12..226
IPR044673	Protein DCL-like	PANTHER	PTHR33415	FAMILY NOT NAMED	coord: 12..226

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg17964-RA	Carg17964-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017126	nucleologenesis
cellular_component	GO:0005634	nucleus