Carg17559 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg17559
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	kunitz trypsin inhibitor 1-like
Location	Carg_Chr12: 10375726 .. 10376408 (+)
RNA-Seq Expression	Carg17559
Synteny	Carg17559

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGTGTAAGTCACTTGTTGCCTTTAGCTACCTATGCCTCGTCATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCTGTCACTGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTGCCACTTCGTTCACAAGAAATAGGTATTATTACATCACAATCGAACTCAAAAACTTTTGATTAGTGATACCATTAGAAAACTCTTGATTATGTTACGAATTCTTAACCATAGGTTTCGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTCGTGTTTCAAGCGTTCTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGAGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

mRNA sequence

ATGTTGTGTAAGTCACTTGTTGCCTTTAGCTACCTATGCCTCGTCATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCTGTCACTGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTGCCACTTCGTTCACAAGAAATAGGTTTCGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTCGTGTTTCAAGCGTTCTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGAGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Coding sequence (CDS)

ATGTTGTGTAAGTCACTTGTTGCCTTTAGCTACCTATGCCTCGTCATGGCCGTGTCCTCTATCGCTCAAAGTCCTCCCGTGCTCGACACCGAGGGCCGACCTTTACAGATCAACGTCGAGTACTTCATCAAGCCTGCTGTCACTGATGTCGCTGGAAATCTCACCCTATTAACCCGCAAAGGCGATCAGTGCCCATTTCATGTTGGCCAAGTGCCACTTCGTTCACAAGAAATAGGTTTCGGGGTCACCTTTACGCCCTACCTACCGGGTGAAGATACGATCAGAGAAGGGAGGGATTTGAAGGTCGTGTTTCAAGCGTTCTCGATCTGCATAACGTCGACTGCATGGAGAGTCGGGGAGACAGATCCTACCACTGGAAGGAGGTTTGTTGAGACCGGGGACGACGACACTGCCACCGGGTACTTTAGGATCGATAGGGACAACTTTGGCGTTTATAATATAGGATGGTGTCCCTCGGATGTCCCTATTAGAGGGAGGCCAAGGTGTGGAAGAGCTGGAATTTTGATTGAGAAAGGCTTGAGGCTGTTGGCTTTGGATGGCTCAGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Protein sequence

MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFVRA

Homology

[Close]

BLAST of Carg17559 vs. NCBI nr
Match: XP_022938134.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata])

HSP 1 Score: 410.2 bits (1053), Expect = 9.9e-111
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK
Sbjct: 2   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 61

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 121

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
           TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL
Sbjct: 122 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 181

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 182 RLLALDGSAFPFEFVRA 198

[Close]

BLAST of Carg17559 vs. NCBI nr
Match: KAG7021121.1 (hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 410.2 bits (1053), Expect = 9.9e-111
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK
Sbjct: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
           TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL
Sbjct: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 181 RLLALDGSAFPFEFVRA 197

[Close]

BLAST of Carg17559 vs. NCBI nr
Match: XP_023521354.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_023538197.1 alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.5 bits (1046), Expect = 6.4e-110
Identity = 194/197 (98.48%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPA+TDVAGNLTLLTRK
Sbjct: 2   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAITDVAGNLTLLTRK 61

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 121

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
           TDPTTGRRFVETGD+DTATGYFRIDRDNFG+YNIGWCPSDVPIRGRPRCGRAGILIEKGL
Sbjct: 122 TDPTTGRRFVETGDNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIEKGL 181

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 182 RLLALDGSAFPFEFVRA 198

[Close]

BLAST of Carg17559 vs. NCBI nr
Match: XP_022965952.1 (kunitz trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 398.3 bits (1022), Expect = 3.9e-107
Identity = 190/197 (96.45%), Postives = 192/197 (97.46%), Query Frame = 0


Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           MLCKSLVAFSYLCLVMA SSIAQSPPVLD EGRPL+INVEYFIKPA+TDVAGNLTLLTRK
Sbjct: 2   MLCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRK 61

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GDQCPFHVGQVPLRSQEIGFGVTFTPYLP ED IREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGE 121

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
            DPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL
Sbjct: 122 ADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 181

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 182 RLLALDGSAFPFEFVRA 198

[Close]

BLAST of Carg17559 vs. NCBI nr
Match: KAG6586275.1 (hypothetical protein SDJN03_19008, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 383.3 bits (983), Expect = 1.3e-102
Identity = 182/182 (100.00%), Postives = 182/182 (100.00%), Query Frame = 0


Query: 16  MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS 75
           MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS
Sbjct: 1   MAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRS 60

Query: 76  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD 135
           QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD
Sbjct: 61  QEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETGDD 120

Query: 136 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 195
           DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV
Sbjct: 121 DTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALDGSAFPFEFV 180

Query: 196 RA 198
           RA
Sbjct: 181 RA 182

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BLAST of Carg17559 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 8.1e-39
Identity = 89/188 (47.34%), Postives = 121/188 (64.36%), Query Frame = 0


Query: 14  LVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPL 73
           L++  +SIAQ   V+DT G P++ + +YFI+PA+T   G+LTL+TR  + CPF+VG  P 
Sbjct: 16  LLVVTTSIAQF--VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCPFNVGLDPD 75

Query: 74  RSQEIGFGVTFTPYLPG--EDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVE 133
             Q  GF V  +P++    ED +R GRDL+V+FQA + C  ST WR+GE D TTGRRF+ 
Sbjct: 76  APQ--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDATTGRRFII 135

Query: 134 TGDDDTATG----YFRI-DRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGLRLLALD 193
           TG DD+  G    +FRI    + G++NI WCP++V    +  CG  GI+ E G  LLALD
Sbjct: 136 TGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVRENGKILLALD 195

Query: 194 GSAFPFEF 195
           GSA P  F
Sbjct: 196 GSALPVAF 197

[Close]

BLAST of Carg17559 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 9.2e-35
Identity = 81/198 (40.91%), Postives = 116/198 (58.59%), Query Frame = 0


Query: 4   KSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQ 63
           ++L+  +++CL +  ++IAQ   VLDT G P++ + EYFI+P +T+  G  T+++R  + 
Sbjct: 8   RTLIILAHVCLFITTTTIAQF--VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR-NES 67

Query: 64  CPFHVGQVPLRSQEIGFGVTFTPYLPGE--DTIREGRDLKVVFQAFSICITSTAWRVGET 123
           CP HVG + L     G  V FTP+ P    D +R  RDL++ FQA S C+ ST WR+GE 
Sbjct: 68  CPLHVG-LELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLGEK 127

Query: 124 DPTTGRRFVETGDDDTATG----YFRI-DRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI 183
           D  +GRR + TG D    G    +FRI +    G+YNI WCP++V    +  CG   +L 
Sbjct: 128 DTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDMLN 187

Query: 184 EKGLRLLALDGSAFPFEF 195
           E G  LLALDG   P  F
Sbjct: 188 ENGKILLALDGGPLPLVF 201

[Close]

BLAST of Carg17559 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 2.7e-34
Identity = 86/202 (42.57%), Postives = 117/202 (57.92%), Query Frame = 0


Query: 1   MLCKSLVAF--SYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLT 60
           M  +SL  F  +++ L+MA +SIAQ   V+DT G P++ + EYFI+PA+T   G  TL+T
Sbjct: 1   MSTRSLTIFILAHVWLLMATTSIAQF--VIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT 60

Query: 61  RKGDQCPFHVGQVPLRSQEIGFGVTFTPYLP--GEDTIREGRDLKVVFQAFSICITSTAW 120
             G  CP HVG +      +G  V FTP+ P   +D +R  RDL+V F   + C  ST W
Sbjct: 61  GNG-PCPLHVG-LDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDW 120

Query: 121 RVGETDPTTGRRFVETGDDDTA---TGYFRI-DRDNFGVYNIGWCPSDVPIRGRPRCGRA 180
           R+GE D T+GRR + TG D+ A     +FRI      G YNI WCP++     + +CG  
Sbjct: 121 RLGEKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTV 180

Query: 181 GILIEKGLRLLALDGSAFPFEF 195
           G++ E G  LLALDG A P  F
Sbjct: 181 GVIRENGKNLLALDGDALPVVF 198

[Close]

BLAST of Carg17559 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 3.6e-15
Identity = 59/195 (30.26%), Postives = 90/195 (46.15%), Query Frame = 0


Query: 14  LVMAVSSIAQS---PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQ 73
           LV+A++++  S     V+D +G  +  +  Y++ P +    G LTL  R G  CP+ + Q
Sbjct: 14  LVLALTAVLASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ 73

Query: 74  VPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQ-AFSICITSTAWRVGETDPTTGRRF 133
                 E G  V F+ +      + E ++L +      +ICI ST WRVGE D    + F
Sbjct: 74  ESSEVDE-GIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYF 133

Query: 134 VETG------DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI-EKGLRL 193
           V  G        D+   +F+I++     Y   +CP      G P+C   GI I E G+R 
Sbjct: 134 VVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRR 193

Query: 194 LALDGSAFPFEFVRA 198
           LAL    F   F +A
Sbjct: 194 LALSDKPFLVMFKKA 205

[Close]

BLAST of Carg17559 vs. ExPASy Swiss-Prot
Match: P07596 (Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-14
Identity = 48/135 (35.56%), Postives = 70/135 (51.85%), Query Frame = 0


Query: 19  SSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQVPLRSQEI 78
           S  A  PPV DT+G  L+ +  Y++  A     G LT+    G  CP  V Q P   Q  
Sbjct: 20  SRAADPPPVHDTDGHELRADANYYVLSANRAHGGGLTMAPGHGRHCPLFVSQDP-NGQHD 79

Query: 79  GFGVTFTPY--LPGEDTIREGRDLKVVFQAFSICITSTAWRVGETDPTTGRRFVETG--D 138
           GF V  TPY   P +  IR   D+++ F+A++ C+ ST W + +++   GRR V TG   
Sbjct: 80  GFPVRITPYGVAPSDKIIRLSTDVRISFRAYTTCLQSTEWHI-DSELAAGRRHVITGPVK 139

Query: 139 DDTATG---YFRIDR 147
           D + +G    FRI++
Sbjct: 140 DPSPSGRENAFRIEK 152

[Close]

BLAST of Carg17559 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 4.8e-111
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK
Sbjct: 2   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 61

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 121

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
           TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL
Sbjct: 122 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 181

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 182 RLLALDGSAFPFEFVRA 198

[Close]

BLAST of Carg17559 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.9e-107
Identity = 190/197 (96.45%), Postives = 192/197 (97.46%), Query Frame = 0


Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           MLCKSLVAFSYLCLVMA SSIAQSPPVLD EGRPL+INVEYFIKPA+TDVAGNLTLLTRK
Sbjct: 2   MLCKSLVAFSYLCLVMAASSIAQSPPVLDIEGRPLEINVEYFIKPAITDVAGNLTLLTRK 61

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GDQCPFHVGQVPLRSQEIGFGVTFTPYLP ED IREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 62  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWRVGE 121

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
            DPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL
Sbjct: 122 ADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 181

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 182 RLLALDGSAFPFEFVRA 198

[Close]

BLAST of Carg17559 vs. ExPASy TrEMBL
Match: A0A6J1DBS1 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.7e-87
Identity = 159/197 (80.71%), Postives = 174/197 (88.32%), Query Frame = 0


Query: 1   MLCKSLVAFSYLCLVMAVSSIAQSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 60
           +L K L  FS L L MA+ S AQSPPVLDT+GRPLQ  VEYFIKPAVTDVAGNLTLLTRK
Sbjct: 4   LLQKPLAIFSCLFLFMAIYSTAQSPPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLLTRK 63

Query: 61  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 120
           GD CPFHVGQVPLRS +IG+ +    +L GEDTIREGRDLKVVFQAFSICITSTAWRVGE
Sbjct: 64  GDPCPFHVGQVPLRSTDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWRVGE 123

Query: 121 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 180
           T+PTTGRR V TG+DDTATG+FRID+ + GVYN+GWCPSDVPIRGRPRCGRAGIL+EKG+
Sbjct: 124 TEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVEKGV 183

Query: 181 RLLALDGSAFPFEFVRA 198
           RLLALDGSAFPFEFVRA
Sbjct: 184 RLLALDGSAFPFEFVRA 200

[Close]

BLAST of Carg17559 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 327.4 bits (838), Expect = 4.1e-86
Identity = 155/196 (79.08%), Postives = 172/196 (87.76%), Query Frame = 0


Query: 4   KSLVA-FSYLCLVMAVSSIAQ--SPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRK 63
           KSL+A FS+LC  MA+ S AQ   PPVLDTEGRPLQ +VEYFIKPA+TDVAGNLTL+TRK
Sbjct: 6   KSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLITRK 65

Query: 64  GDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVGE 123
           GDQCPF+VGQVPL SQEIGFGVT TPYL G+DTIREGRDLK VFQAF+ICIT T W+VGE
Sbjct: 66  GDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQWKVGE 125

Query: 124 TDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKGL 183
            DP +GRRFV+ G+D+TATGYFRID+ N GVYNIGWCPSDVP +GRPRCG AGILIEKG+
Sbjct: 126 EDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILIEKGV 185

Query: 184 RLLALDGSAFPFEFVR 197
           R LALDG AFPFEFVR
Sbjct: 186 RFLALDGPAFPFEFVR 201

[Close]

BLAST of Carg17559 vs. ExPASy TrEMBL
Match: A0A5A7V7Y2 (Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00540 PE=3 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.4e-81
Identity = 150/197 (76.14%), Postives = 164/197 (83.25%), Query Frame = 0


Query: 4   KSLVA-FSYLCLVMAVSSIA---QSPPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTR 63
           KSL A FS LC  MA+ S A     PPVLDTEGRPLQ + EYFIKPA+TDVAGNLTL+TR
Sbjct: 6   KSLFAIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLTLITR 65

Query: 64  KGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRVG 123
           KGDQCPF+VGQVPL SQE GFGV+  PY  GEDTIREGRDLK VFQ F+ICIT T W+VG
Sbjct: 66  KGDQCPFYVGQVPLFSQETGFGVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQWKVG 125

Query: 124 ETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEKG 183
           E DP TGRRFV+ G D+TATGYFRID+ + GVYNIGWCPSDVPI+GRPRCG AGILIEKG
Sbjct: 126 EADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGILIEKG 185

Query: 184 LRLLALDGSAFPFEFVR 197
           +R LALDG AFPFEFVR
Sbjct: 186 VRFLALDGPAFPFEFVR 202

[Close]

BLAST of Carg17559 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 83.2 bits (204), Expect = 2.6e-16
Identity = 59/195 (30.26%), Postives = 90/195 (46.15%), Query Frame = 0


Query: 14  LVMAVSSIAQS---PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLTRKGDQCPFHVGQ 73
           LV+A++++  S     V+D +G  +  +  Y++ P +    G LTL  R G  CP+ + Q
Sbjct: 14  LVLALTAVLASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAGRGGQPCPYDIVQ 73

Query: 74  VPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQ-AFSICITSTAWRVGETDPTTGRRF 133
                 E G  V F+ +      + E ++L +      +ICI ST WRVGE D    + F
Sbjct: 74  ESSEVDE-GIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQYF 133

Query: 134 VETG------DDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILI-EKGLRL 193
           V  G        D+   +F+I++     Y   +CP      G P+C   GI I E G+R 
Sbjct: 134 VVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVRR 193

Query: 194 LALDGSAFPFEFVRA 198
           LAL    F   F +A
Sbjct: 194 LALSDKPFLVMFKKA 205

[Close]

BLAST of Carg17559 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 48.5 bits (114), Expect = 7.1e-06
Identity = 43/165 (26.06%), Postives = 66/165 (40.00%), Query Frame = 0


Query: 2   LCKSLVAFSYLCLVMAVSSIAQSPP---VLDTEGRPLQINVEYFIKPAVTDVAGNLTLLT 61
           L  S +  + L  +   +S A + P   VLD  G P+Q NV+Y+I PA     G L    
Sbjct: 4   LTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLIPSN 63

Query: 62  RK---GDQCPFHVGQVPLRSQEI-GFGVTFTPYLPGEDTIREGRDLKVVFQA-FSICITS 121
           R     D C  ++  V   S  + G  VTF+P       ++    L + F +   +C  S
Sbjct: 64  RNLSTQDLC-LNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCPDS 123

Query: 122 TAWRVGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCP 159
             WR+  +           G       +F+I  D    Y + +CP
Sbjct: 124 KVWRIDHSVQLRKSFVSIGGQKGKGNSWFQIQEDG-DAYKLMYCP 166

The following BLAST results are available for this feature:

BLAST of Carg17559 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022938134.1	9.9e-111	100.00	alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata]	[more]
KAG7021121.1	9.9e-111	100.00	hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023521354.1	6.4e-110	98.48	alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_0235381...	[more]
XP_022965952.1	3.9e-107	96.45	kunitz trypsin inhibitor 1-like [Cucurbita maxima]	[more]
KAG6586275.1	1.3e-102	100.00	hypothetical protein SDJN03_19008, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Carg17559 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	8.1e-39	47.34	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	9.2e-35	40.91	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
A0A072TH68	2.7e-34	42.57	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
Q8RXD5	3.6e-15	30.26	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P07596	1.4e-14	35.56	Alpha-amylase/subtilisin inhibitor OS=Hordeum vulgare OX=4513 PE=1 SV=2	[more]

BLAST of Carg17559 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FD70	4.8e-111	100.00	alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLP6	1.9e-107	96.45	kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3...	[more]
A0A6J1DBS1	1.7e-87	80.71	miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1	[more]
A0A0A0LGB4	4.1e-86	79.08	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1	[more]
A0A5A7V7Y2	1.4e-81	76.14	Endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo var. makuwa O...	[more]

BLAST of Carg17559 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73260.1	2.6e-16	30.26	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	7.1e-06	26.06	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 26..197 e-value: 8.9E-22 score: 88.3
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 27..195 e-value: 1.1E-36 score: 126.4
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 5..197
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 27..43
None	No IPR available	GENE3D	2.80.10.50		coord: 23..197 e-value: 5.0E-39 score: 135.8
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 5..197
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 25..196

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg17559-RA	Carg17559-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity