Carg16449 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg16449
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	diacylglycerol kinase
Location	Carg_Chr12: 7519968 .. 7525892 (-)
RNA-Seq Expression	Carg16449
Synteny	Carg16449

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTTTTTTTCCTCTCTCTCTGTCTCTTGTGACTTCGCCGGAATGTGTTTGATTTGATTTCTTCACTCGATCGCCAAGTCCTTATGTATTTATCCAGAGGAACAAGAGGCTAATCCAACACTTCGTGATTCTGTGAAGGAAAAAGAGAGAGGAGAGGGAGGGAAAGTGATGGATTCGCCAACGTCGGGAGGCGAAGGCGATTCAACAACTCGAATCGCGGCTCAATCTTCGGTGGTGGACTCGTTCAAGGGATGTGGATTCACGGGGATCAAAATCGACAAGGATGAGTTGCGGAAGCAGCTCCGGATGCCGCAATACCTTCGTTATGCTATGCGAGATTCCATTCGGCTTCAGGACCCTGGCGCCGGAGAGACCTGGTTGCCTGGTGCTAGATCTATCTCTGGCTCCTCCGTTGTTCCAGAGATTAACGATGGTCCGCCTGAATCGCCGATGGTTGTGTTCATTAATCCTCGAAGTGGTGGTCGCCATGGTCCCTTGCTTAAGGAGAGGCTCCAGATGCTAATTAGCGAAGAACAGGTCCATACATTTCTGAAACTTTCCCCTCCCTCTCTTGCTTTTGAATTTTGTTTAATGCTGTTCAACGATGCGTGTTCATACAAACCTAGGATTTGTAAAATGTTGTTAATCAAAATTTGATTCGGAGAGTTGGATGTGTTGTTCTTGTTCAGATCAATAATATCTAGTCAGTGATTGAAATCTCCATAGCTAATGGTTCTGAACGTTGGTCCATAATTTCTGTGAAGGTTTTTGACCTAACAGATGTGAAGCCCCATGAATTTGTCCGGTATGGATTGAGATGCCTCGAGTTATTGGCTGGGCTTGGTGATGCTTGTGCTAAAGAGATCCGTGAGAAGATGAGGGTCATGGTATGATTAAAGATCTGAAGCTATATGAATTTTGGTTATGGTGAAAACTAACTGATTTATCCACAGGTTGCTGGAGGTGATGGCACTGTTGGTTGGGTTCTTGGTTGTCTTTTAGACCTCGAGAAAAGCGGGCGGCTGCCCGTTCCACCAGTAGGAATAATTCCTCTTGGTACTGGCAATGACTTATCCAGAACCTTTGGCTGGGTGAGTTCTCTTATTTCTCATGAAAATTCATATCATATTTACATATCTGATAATGTCATGAGCTGTAGGATTGAATGCTCTTTTCAGGGTGGTTCTTTTCCTTTTGCTTGGAAGTCAGCCATCAAAAGATCCCTTGACAGGGCAACCACTGGTCAGATTCGTAGATTGGATAGGTACTATTTTTCTCGGTTTTAAGCATAATTATGGCATTGTGTGATCTCACATCGGTTGAGGAGGAGAACGAAGCACCCTTTATAAGGGTGGCATTGTGAGATCCCACATCGGTTGGGGAGGAGAACGAAGCACCCTTTACAAGGGTGTTGAAACCTCTCCTTAACAGAGGCGTTTTAGTTTTAAAAACTTTGAGGGGAAGCCCGAAATGGAAAGTCAAAAGAGGACAATATCTGCTAACGGTGGGCCTGGGCCGTTACGAATGGTATCAGAGCCAGACACGGGGCAATGTGTCGGCGAATAGGCTGTTCCCGAAGGGGGTAGATATGAGGTGGTGTGTCAGTAAGGATGCTGAACTCCGAAGGTGATGGATTTGGTGGTGGTCCCACATCGATGGGAGAAAGGAACGAGTGCTAACGAGGATGCTGGGCCCTAAACGGGGTGGATTGTGAGATCCCACGTCTTTGATAGGAGAACGAAACACCCTTTATAAAGGTGTTGAAACCTCTCCCTAGCAGAAAAGTTTTAAAAACCTTGAGGGAAGCCCGAAATAGAAAGCCCAAAAGACAATATCTGTTAGCGTGGGCTGGGCCGTTACAGGCATGGTGTGCTGGTGATTGCAGGTTTAGGTTTGTAAATGTGAATGTTTTGGTAGTATAGTTCTCTGTAAATGCAGTGGCTGAGAGTGCTTGGTGCCTCTGCATAGTGAACGCATCAGTGAAACAATTTATGGCCCCTCATAAGTCTGAATCTTGTGATGCAGTTGGCATGTTACTCTAGCAACCCCATCTACTAAAATCGTGAAATTACCACATTGCATGAAACCTACGGAAGTCCATGCTCTTGATGAGGTTTGTGCACTTCTTCAACGTTTTTTTAATACTTTCACAGTCTAGCTGAATGAAAACACTCTGGATGGGCTCTGGTGCCTTACCATTGACACTGCCCTTTAATCGTTCATCCATTTTTCTTTTAATCATTCAACACTACCCTTTAATTTATCTTATCTTTCTTTCTGTTATCCCTTGTACATTTAGTGGTTAGTGGACAAGCAAAAATATAAGTGCTAATCTTTAAAATGCTGCATCTTCTTTTCCAAATAATCTTGTGACAACAAAGATTTGTATCTCAGAGGTAGAAGTGTTTTTTTTTTTTAATTTATTTTTGTGGCCGCAGAGCTTGGAAATCGAGGAGTCATTACCAGAGAAAGCCTACTTCCACGAAGGAGTATTTTATAATTACTTCAGCATAGGTATATTAGGCGTTTACATTTAAATAGCCAATGACACTCTCATGAATGATAAATCCTGAAGAAACATTATTTACCTGAAAATTTGAAGTTAATTCTGCCATTTACGCAAGTGTCAATATAAAAGAGTATGTATGTTGAACTTGATTCTACGGTCAACATCAAAGCGGTTAGTACGCAGATATTGGCTCTTTGTCAATATTGAAGAGGTTTTTAGGCAGAAACTAATTTTCAAGTAGTTGCAGTAATATTCTTTCTCTCTGTCTTTTATTAAGTAATGGTGCAATTATTTCCTTTCTCAGCTAAAACTATATATTATACTGTCATTGAACTGCCTGAAATGGGCTCGTGTAACAATGGCTAACTTTCTAGCTTCAAGCAATATTAGCTCAATTTTGTTTTCTGAAGGGATAATGTATGCTTTGACATATTTGGTTGGTATTGAAATGCAGTATTGTACTAATGTGGTTTGCAAATCTAAATAAATGCACCTTGTGGATATATCTCTCTATCTCTCTCAGTCAAGGGAGTTAGCTTTAGATCGCAGGTGCAATTTTATTTGTTAGTTTAACAGGGGTGCATTTTGTTTATTTTCATATCGCATGGTTACAAAAAATGCAATCATTTAATGATCTTCATTTGATTGTTTGATCACTTGTTTGTAAGTAGGTTTTTTTATTTTGTTCATGCAGGAATGGATGCTCAAGTTGCTTATGGCTTCCACCAACTTCGGGATGAAAAACCCTACCTCGCACAAGGTCCAATTTCTAACAAGGTTGGGCTTTGAACTTTAAAGTTATTTAGAAATTTGGAGTAGTTTGCTGCAAGTTTCCTTTAATCACTTTATTTTATGAATTAAACCGACAAACAATTAATTTTGAATACTAATATAAAAACAAAGCCTTCTATAGAATGAATGAGATCAACACATCCAAACTAGAACGAACGCAACATCTGTGGATACGAGGGTAGTAAAATCTTTGAGCATCCGAAGAATAAGGTGGAGTCTCCTGGAAAATCTGGAGGGATGTTGGTCCTTCATTCCTATGGGGTGAAAGGAAAATCAGGGTGGATGAGGTGAGGGAGGTTATTCTTTATCAATCAAAACTGAAAAAGCCTTTTGAGAATGCAAAATTGTGGTAACTCTGAAAATTTTTGGGGAATGATAATGCTCCAGGACTTAAGTTGATCTCTTAAATATGTTCAATGGTTTTTATTGAAACGTAAGGATGAATTCAACTGATAGAGATCTCCTATTACAGCTGATTTATTCTGGCTATAGTTGTAGTCAGGGCTGGTTCTTCACGCCTTGCATTAGTGATCCGAGTTTAAGGTTAGATATTCTACACCGTTTGGGACTGTTGCTTTGTGATTTGGATATGAGTTTTTCAAATATAGAAAACAAGTAGGTTTGTATTTGCTAAACAGAGTTTCTAAGAAAATTTTCTGGTTGTAGGGGCCTTAAACACATCCTAAGGATGCATGTCAAAAAGGTCAATTGTTCCGATTGGGAGGAGGTTTTAATCCCCTCGAGGTATCTATCATGCGCTCTTTGCTTGGCTTAACTTGCATTTGTAATGGTATTTTTGAGTTCATGCATCCAATATCGTATTGTTGTTGAAATAAACAGAATTTTGTGTATTGAACTGTTTCTTTGAAATGAATATGATAGTGATTAACAGGGTCGATGTCATGCAGCAGCCTAATTGTTGTTGCGGCCAATAAAAAAACCTAACCTCTAAAACTAGCTGAATCTTTAGATTATGAGCCTTATCATTCTTGCAGTGGTTTGAAGTGTTTTAGTATAGGAAATGTGTAGCCAGGACCCATTTTGTTGGGATTAAAGTCTGTTTAAGTTGAGTTCGAATTTGAGGGAAGATGGTTTTCGCTCCCCAAATTTATCTTATTATTATTATTAGAGAAAGGGGAGACTAACAAGGTTTGGAACCCTACTCCCCAATCCATATATCAATCTTGTAAGCAAGTTGGTGCAGCGCTTTCGCTTTAAATCAATCTCACTCATTTGGTTTGTTCGGTCAAATCAGCTCTCTTGCCTGAATGGTTTTTGCTTCCCAAATTTTTAGGCATTTAACTTGAATCATTTTGTGCAGTGTAAGATCTCTGGTTGCGTTGAATCTCAACAACTATGGTAGTGGGAGACATCCATGGGGAAATCTTACGCCAGAGTACATGGAAAGGGTACATTGTTTACTTACCTTTTCTCTTTACATGATGAAACGTTTCTTATCTGCACATATCACCCAGATTTCTAATTTTTTTAACTGGATTCTAGCCCAATTACATTTGACAGGATATAATTTCTGTTTATTGCACTAGTTTTGGCAATTTTCTTATAGCTTGGCCATTTTTGTTGCGATTCCCTAGGAACAGGAGTTCTCTAGTCTCTCTTGCTGCTATAAATGAATACCCAAACCTTTCTCTATCTCTATTTGTAACGGCCCAAGCCTATCGCTAGCAGATATTATCCTTTTTGGGCTTTCCCTTCCCCTCAAGATTTTTAAAACACTTCTGCTGGGAGAGGTTTCTACACCCGTATAAAGGGTGTGTCATTCTCCTCCTCAACCTATGTGAGATCTCACAATCCACCCTCATTCAGGGCCCAGCATCCTCGTTGGCACTCGTTCCTTTTTCTAATCGATGTGAGACCACTACCAAATTCACCCCCCTTCGGGGCCCAACGTCCTCGCTGGCACTCGGTCCTTTCTCCAATCGATGTGGGACCCCACCAAATCCACCCATCTTCGGGGCCTAGCCCTCATGTCCACCCTCCTTCGAGACTCAGCCTCCTTGCTGGCAAATCGCCTGGTGTCTGACTCTAATACCATTTCTAACGGCCCAAACCCACCGCTAGCAGATATTGTCATCTTTGGACTTCACCTCTTGGGATTCCCCTCAAGATTTTTAAAACAACTATACTAGGGAGTGGTTTTCACACCCTTATAAAGGGTGTGTCTTTCTCCTCCCCAACCGACGTGGGATCTCACACTAGTTCTTTAAGGCTACGACTAGAGTTTACAAATTTTCTTAGAAATATTTTACTTTCAGAGAGGATTTGTTGAGGCACATGTTGATGATGGGCTTCTAGAAGTGTTTGGTCTAAAGCAAGGGTGGCATGCATCACTTGTTATGGGAGAGGTCATCTCAGCTAAACACATAGTTCAGGTAATGATATTGTGTTCGATTCATTCATCAAATTGCTACAAATTGAAACACTTTGGTAAAAATTGTGGGGTTGCAGGCTGCAGCAATTCGGTTTGAGCTTCGAGGTGGAGAATGGAAACACGCATTTCTACAGATGGATGGAGAGCCATGGAAGCAACCCATGAGCAGAGAATGTTTGACTTACGTTGAAATCAAAAGGGTACCTTCCCAATCACTTATGATCAATGGAGAA

mRNA sequence

TTTTTTTTTCCTCTCTCTCTGTCTCTTGTGACTTCGCCGGAATGTGTTTGATTTGATTTCTTCACTCGATCGCCAAGTCCTTATGTATTTATCCAGAGGAACAAGAGGCTAATCCAACACTTCGTGATTCTGTGAAGGAAAAAGAGAGAGGAGAGGGAGGGAAAGTGATGGATTCGCCAACGTCGGGAGGCGAAGGCGATTCAACAACTCGAATCGCGGCTCAATCTTCGGTGGTGGACTCGTTCAAGGGATGTGGATTCACGGGGATCAAAATCGACAAGGATGAGTTGCGGAAGCAGCTCCGGATGCCGCAATACCTTCGTTATGCTATGCGAGATTCCATTCGGCTTCAGGACCCTGGCGCCGGAGAGACCTGGTTGCCTGGTGCTAGATCTATCTCTGGCTCCTCCGTTGTTCCAGAGATTAACGATGGTCCGCCTGAATCGCCGATGGTTGTGTTCATTAATCCTCGAAGTGGTGGTCGCCATGGTCCCTTGCTTAAGGAGAGGCTCCAGATGCTAATTAGCGAAGAACAGGTTTTTGACCTAACAGATGTGAAGCCCCATGAATTTGTCCGGTATGGATTGAGATGCCTCGAGTTATTGGCTGGGCTTGGTGATGCTTGTGCTAAAGAGATCCGTGAGAAGATGAGGGTCATGGTTGCTGGAGGTGATGGCACTGTTGGTTGGGTTCTTGGTTGTCTTTTAGACCTCGAGAAAAGCGGGCGGCTGCCCGTTCCACCAGTAGGAATAATTCCTCTTGGTACTGGCAATGACTTATCCAGAACCTTTGGCTGGGGTGGTTCTTTTCCTTTTGCTTGGAATTGGCATGTTACTCTAGCAACCCCATCTACTAAAATCGTGAAATTACCACATTGCATGAAACCTACGGAAGTCCATGCTCTTGATGAGAGCTTGGAAATCGAGGAGTCATTACCAGAGAAAGCCTACTTCCACGAAGGAGTATTTTATAATTACTTCAGCATAGGAATGGATGCTCAAGTTGCTTATGGCTTCCACCAACTTCGGGATGAAAAACCCTACCTCGCACAAGGTCCAATTTCTAACAAGCTGATTTATTCTGGCTATAGTTGTAGTCAGGGCTGGTTCTTCACGCCTTGCATTAGTGATCCGAGTTTAAGGGGCCTTAAACACATCCTAAGGATGCATGTCAAAAAGGTCAATTGTTCCGATTGGGAGGAGGTTTTAATCCCCTCGAGTGTAAGATCTCTGGTTGCGTTGAATCTCAACAACTATGGTAGTGGGAGACATCCATGGGGAAATCTTACGCCAGAGTACATGGAAAGGGCTGCAGCAATTCGGTTTGAGCTTCGAGGTGGAGAATGGAAACACGCATTTCTACAGATGGATGGAGAGCCATGGAAGCAACCCATGAGCAGAGAATGTTTGACTTACGTTGAAATCAAAAGGGTACCTTCCCAATCACTTATGATCAATGGAGAA

Coding sequence (CDS)

ATGGATTCGCCAACGTCGGGAGGCGAAGGCGATTCAACAACTCGAATCGCGGCTCAATCTTCGGTGGTGGACTCGTTCAAGGGATGTGGATTCACGGGGATCAAAATCGACAAGGATGAGTTGCGGAAGCAGCTCCGGATGCCGCAATACCTTCGTTATGCTATGCGAGATTCCATTCGGCTTCAGGACCCTGGCGCCGGAGAGACCTGGTTGCCTGGTGCTAGATCTATCTCTGGCTCCTCCGTTGTTCCAGAGATTAACGATGGTCCGCCTGAATCGCCGATGGTTGTGTTCATTAATCCTCGAAGTGGTGGTCGCCATGGTCCCTTGCTTAAGGAGAGGCTCCAGATGCTAATTAGCGAAGAACAGGTTTTTGACCTAACAGATGTGAAGCCCCATGAATTTGTCCGGTATGGATTGAGATGCCTCGAGTTATTGGCTGGGCTTGGTGATGCTTGTGCTAAAGAGATCCGTGAGAAGATGAGGGTCATGGTTGCTGGAGGTGATGGCACTGTTGGTTGGGTTCTTGGTTGTCTTTTAGACCTCGAGAAAAGCGGGCGGCTGCCCGTTCCACCAGTAGGAATAATTCCTCTTGGTACTGGCAATGACTTATCCAGAACCTTTGGCTGGGGTGGTTCTTTTCCTTTTGCTTGGAATTGGCATGTTACTCTAGCAACCCCATCTACTAAAATCGTGAAATTACCACATTGCATGAAACCTACGGAAGTCCATGCTCTTGATGAGAGCTTGGAAATCGAGGAGTCATTACCAGAGAAAGCCTACTTCCACGAAGGAGTATTTTATAATTACTTCAGCATAGGAATGGATGCTCAAGTTGCTTATGGCTTCCACCAACTTCGGGATGAAAAACCCTACCTCGCACAAGGTCCAATTTCTAACAAGCTGATTTATTCTGGCTATAGTTGTAGTCAGGGCTGGTTCTTCACGCCTTGCATTAGTGATCCGAGTTTAAGGGGCCTTAAACACATCCTAAGGATGCATGTCAAAAAGGTCAATTGTTCCGATTGGGAGGAGGTTTTAATCCCCTCGAGTGTAAGATCTCTGGTTGCGTTGAATCTCAACAACTATGGTAGTGGGAGACATCCATGGGGAAATCTTACGCCAGAGTACATGGAAAGGGCTGCAGCAATTCGGTTTGAGCTTCGAGGTGGAGAATGGAAACACGCATTTCTACAGATGGATGGAGAGCCATGGAAGCAACCCATGAGCAGAGAATGTTTGACTTACGTTGAAATCAAAAGGGTACCTTCCCAATCACTTATGATCAATGGAGAA

Protein sequence

MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIRLQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLISEEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAWNWHVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVAYGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNCSDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMERAAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKRVPSQSLMINGE

Homology

[Close]

BLAST of Carg16449 vs. NCBI nr
Match: KAG7020845.1 (Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 907.1 bits (2343), Expect = 5.7e-260
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60
           MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR
Sbjct: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60

Query: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120
           LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS
Sbjct: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAWNWHVTLATPSTKIVKLPHCMKP 240
           DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAWNWHVTLATPSTKIVKLPHCMKP
Sbjct: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAWNWHVTLATPSTKIVKLPHCMKP 240

Query: 241 TEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVAYGFHQLRDEKPYLAQGPISN 300
           TEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVAYGFHQLRDEKPYLAQGPISN
Sbjct: 241 TEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVAYGFHQLRDEKPYLAQGPISN 300

Query: 301 KLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNCSDWEEVLIPSSVRSLVALNL 360
           KLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNCSDWEEVLIPSSVRSLVALNL
Sbjct: 301 KLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNCSDWEEVLIPSSVRSLVALNL 360

Query: 361 NNYGSGRHPWGNLTPEYMERAAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIK 420
           NNYGSGRHPWGNLTPEYMERAAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIK
Sbjct: 361 NNYGSGRHPWGNLTPEYMERAAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIK 420

Query: 421 RVPSQSLMINGE 433
           RVPSQSLMINGE
Sbjct: 421 RVPSQSLMINGE 432

[Close]

BLAST of Carg16449 vs. NCBI nr
Match: XP_022937846.1 (diacylglycerol kinase 7-like [Cucurbita moschata])

HSP 1 Score: 874.0 bits (2257), Expect = 5.3e-250
Identity = 431/491 (87.78%), Postives = 432/491 (87.98%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60
           MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR
Sbjct: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60

Query: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120
           LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS
Sbjct: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW--------------------NW 240
           DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW                    +W
Sbjct: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRRLDSW 240

Query: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300
           HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA
Sbjct: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360
           YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC
Sbjct: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360

Query: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER-------------------- 420
           SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER                    
Sbjct: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMERRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 -------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 433
                              AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR
Sbjct: 421 GWHASLVMGEVISAKHIVQAAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 480

[Close]

BLAST of Carg16449 vs. NCBI nr
Match: XP_023538307.1 (diacylglycerol kinase 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 872.8 bits (2254), Expect = 1.2e-249
Identity = 430/491 (87.58%), Postives = 432/491 (87.98%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60
           MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR
Sbjct: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60

Query: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120
           LQDPGAGETWLPGARSISGSSVVPEINDGPP+SPMVVFINPRSGGRHGPLLKERLQMLIS
Sbjct: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPDSPMVVFINPRSGGRHGPLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW--------------------NW 240
           DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW                    +W
Sbjct: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRRLDSW 240

Query: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300
           HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA
Sbjct: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360
           YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC
Sbjct: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360

Query: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER-------------------- 420
           SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER                    
Sbjct: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMERRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 -------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 433
                              AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR
Sbjct: 421 GWHASLVMGEVISAKHIVQAAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 480

[Close]

BLAST of Carg16449 vs. NCBI nr
Match: XP_022969690.1 (diacylglycerol kinase 7-like [Cucurbita maxima])

HSP 1 Score: 867.5 bits (2240), Expect = 5.0e-248
Identity = 429/491 (87.37%), Postives = 430/491 (87.58%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60
           MDSPTSGGEGDSTTRIAAQSSVVDS KG GFTGIKIDKDELRKQLRMPQYLRYAMRDSIR
Sbjct: 1   MDSPTSGGEGDSTTRIAAQSSVVDSLKGFGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60

Query: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120
           LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS
Sbjct: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW--------------------NW 240
           DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW                    +W
Sbjct: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAWKSAIKKSLDRATTGQIRRLDSW 240

Query: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300
           HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA
Sbjct: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360
           YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC
Sbjct: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360

Query: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER-------------------- 420
           SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER                    
Sbjct: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMERRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 -------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 433
                              AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR
Sbjct: 421 GWHASLVMGEVISAKHIVQAAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 480

[Close]

BLAST of Carg16449 vs. NCBI nr
Match: XP_038890957.1 (diacylglycerol kinase 7-like [Benincasa hispida])

HSP 1 Score: 759.2 bits (1959), Expect = 1.9e-215
Identity = 371/491 (75.56%), Postives = 405/491 (82.48%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60
           M+SP+S GEGDSTTRIAA+SS+++SFKGCG TG++IDK+ELRK+LRMPQYLR+AMRDSIR
Sbjct: 1   MNSPSSRGEGDSTTRIAARSSMIESFKGCGLTGLRIDKEELRKKLRMPQYLRFAMRDSIR 60

Query: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120
           LQDP AGE+ LPGA SISGS+ VPE +DGPP SPMVVFINPRSGGRHG LLKERLQMLIS
Sbjct: 61  LQDPSAGESRLPGANSISGSAFVPENDDGPPASPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180
           EEQ+FDLTDVKPHEFV+YGLRCLELLAGLGDACAK++REKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQIFDLTDVKPHEFVKYGLRCLELLAGLGDACAKDVREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW--------------------NW 240
           +LEK  RLPVPPVG+IPLGTGNDLSRTFGWGGSFPFAW                    +W
Sbjct: 181 ELEKKERLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLDSW 240

Query: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300
           HV L+TPS + V+LPHCMKPTE HALDESLEIEE+L EKA  HEGVFYNYFSIGMDAQVA
Sbjct: 241 HVILSTPSGENVELPHCMKPTEEHALDESLEIEEALSEKASCHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360
           YGFH LR+EKPYLAQGPI+NKLIYSGYSC QGWFFTPC SDP LRGLK+ILRMHVKKVNC
Sbjct: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPRLRGLKNILRMHVKKVNC 360

Query: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER-------------------- 420
           SDWE++LIPSSVRSLVALNLNNYGSGRHPWGNLTPEYME+                    
Sbjct: 361 SDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGLKQ 420

Query: 421 -------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 433
                              AAAIRFELRGGEWK AFLQMDGEPWKQPMS E  T+VEIK+
Sbjct: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSDEFSTFVEIKK 480

[Close]

BLAST of Carg16449 vs. ExPASy Swiss-Prot
Match: F4JQ95 (Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1)

HSP 1 Score: 561.2 bits (1445), Expect = 1.0e-158
Identity = 277/500 (55.40%), Postives = 348/500 (69.60%), Query Frame = 0


Query: 2   DSPTSGGEGDSTTRIAAQSSV----VDSFKGCGFTG---IKIDKDELRKQLRMPQYLRYA 61
           ++P S GE  +T  +AA+ S       + +GCGF     + IDK+ELR +L MP+YLR A
Sbjct: 3   ETPRSVGEASTTNFVAARPSAKTDDAVTMRGCGFANLALVGIDKEELRGRLAMPEYLRIA 62

Query: 62  MRDSIRLQDPG--AGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLK 121
           MRD I+ +D    +    LPG  +           D  P +PMVVFINP+SGGRHGP+LK
Sbjct: 63  MRDCIKRKDSTEISDHLLLPGGAAA----------DMAPHAPMVVFINPKSGGRHGPVLK 122

Query: 122 ERLQMLISEEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTV 181
           ERLQ L++EEQVFDLT+VKPHEFVRYGL CL+ LA  GD CA+E REK+R+MVAGGDGTV
Sbjct: 123 ERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAKGDECARECREKIRIMVAGGDGTV 182

Query: 182 GWVLGCLLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW-------------- 241
           GWVLGCL +L K G+  +PPVG+IPLGTGNDLSR+F WGGSFPFAW              
Sbjct: 183 GWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFSWGGSFPFAWRSAMKRTLHRATLG 242

Query: 242 ------NWHVTLATPSTKIVKLPHCMKPT-EVHALDESLEIEESLPEKAYFHEGVFYNYF 301
                 +W + ++ PS ++V  P+ +KPT E  ALD++L+ +  +P KA  +EGVFYNYF
Sbjct: 243 SIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQALDADGDVPPKAKSYEGVFYNYF 302

Query: 302 SIGMDAQVAYGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHIL 361
           SIGMDAQVAYGFH LR+EKPYLAQGP++NK+IYS YSC+QGWF TPC+++P+LRGL++I+
Sbjct: 303 SIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYSCTQGWFCTPCVNNPALRGLRNIM 362

Query: 362 RMHVKKVNCSDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER----------- 421
           ++H+KK NCS+WEE+ +P SVRS+V LNL NYGSGRHPWGNL P+Y+E+           
Sbjct: 363 KIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRHPWGNLRPKYLEKRGFVEAHCDDG 422

Query: 422 ----------------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRE 433
                                       AAAIRFELRGG+WK+AFLQMDGEPWKQPM  +
Sbjct: 423 LIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELRGGDWKNAFLQMDGEPWKQPMKSD 482

[Close]

BLAST of Carg16449 vs. ExPASy Swiss-Prot
Match: Q8VZG1 (Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1)

HSP 1 Score: 554.3 bits (1427), Expect = 1.2e-156
Identity = 273/496 (55.04%), Postives = 338/496 (68.15%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDS--FKGCGFTG---IKIDKDELRKQLRMPQYLRYAM 60
           MDSP S  +      +A++ S  DS   +GCG      + +DK ELR++L MP+YLR AM
Sbjct: 1   MDSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAM 60

Query: 61  RDSIRLQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERL 120
           RD I+ +D  A    +P    + G +V     D  P +PMVVFINP SGGRHGP+LKERL
Sbjct: 61  RDCIKRKDSSA----IPDHLLLPGGAVA----DMAPHAPMVVFINPNSGGRHGPVLKERL 120

Query: 121 QMLISEEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWV 180
           Q L+SEEQVFDLT+VKPHEFVRYGL CLE +A  GD CAKE R ++R+MVAGGDGTVGWV
Sbjct: 121 QQLMSEEQVFDLTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWV 180

Query: 181 LGCLLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW----------------- 240
           LGCL +L K G+  +PPVG+IPLGTGNDLSR+FGWGGSFPFAW                 
Sbjct: 181 LGCLGELNKDGKSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVA 240

Query: 241 ---NWHVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGM 300
              +W + ++ PS ++V  P+ +KP E + LD+ L+     P  A  +EGVFYNY SIGM
Sbjct: 241 RLDSWKILVSMPSGEVVDPPYSLKPAEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGM 300

Query: 301 DAQVAYGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHV 360
           DAQVAYGFH LR+ KPYLAQGPISNK+IYS + CSQGWF TPC++DP LRGL++I+++H+
Sbjct: 301 DAQVAYGFHHLRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHI 360

Query: 361 KKVNCSDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER--------------- 420
           KKVNCS WEE+ +P +VRS+VALNL++YGSG HPWGNL P+Y+E+               
Sbjct: 361 KKVNCSQWEEIAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEI 420

Query: 421 ------------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTY 433
                                   AAA+RFELRGG+W+ AFLQMDGEPWKQPMS E  T+
Sbjct: 421 FGFKQGWHASFVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTF 480

[Close]

BLAST of Carg16449 vs. ExPASy Swiss-Prot
Match: Q1PDI2 (Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 7.9e-148
Identity = 264/499 (52.91%), Postives = 336/499 (67.33%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKG------CGFTGIKIDKDELRKQLRMPQYLRYA 60
           M+SP+ G     T R+  + S +DSF             I++ K ELR+++ +PQYLR A
Sbjct: 1   MESPSIG--DSLTARMIPRHSSLDSFGAMKVSLLVNLASIRVSKAELRQRVMLPQYLRIA 60

Query: 61  MRDSIRLQDPGAGETWLPGARSISGSSVVP--EINDGPPESPMVVFINPRSGGRHGPLLK 120
           +RD I  +D              + SSV P  E N   PE P++VF+NP+SGGR GPL+K
Sbjct: 61  IRDCILRKDDSFD----------ASSSVAPPLENNALTPEVPLMVFVNPKSGGRQGPLIK 120

Query: 121 ERLQMLISEEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTV 180
           ERLQ LISEEQV+DLT+VKP+EF+RYGL CLE  A  GD CAKEIREKMR++VAGGDGTV
Sbjct: 121 ERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIREKMRIVVAGGDGTV 180

Query: 181 GWVLGCLLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW-------------- 240
           GWVLGCL +L    RLPVPPV I+PLGTGNDLSR+FGWGGSFPFAW              
Sbjct: 181 GWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAWKSAIKRTLHRASVA 240

Query: 241 ------NWHVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFS 300
                 +W++ +  PS +IV  P+ +K T+   +D++LEIE  +P     +EGVFYNYFS
Sbjct: 241 PISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQNLEIEGEIPPSTNGYEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILR 360
           IGMDAQVAYGFH LR+EKPYLA GPI+NK+IYSGY CSQGWF T CI+DP LRGLK+I+ 
Sbjct: 301 IGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTHCINDPGLRGLKNIMT 360

Query: 361 MHVKKVNCSDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER------------ 420
           +H+KK++ S+WE+V +P SVR++VALNL++YGSGR+PWGNL  +Y+E+            
Sbjct: 361 LHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDYLEKRGFVEAQADDGL 420

Query: 421 ---------------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSREC 433
                                      AAAIR E+RGG+WK AF+QMDGEPWKQPM+R+ 
Sbjct: 421 LEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFMQMDGEPWKQPMTRDY 480

[Close]

BLAST of Carg16449 vs. ExPASy Swiss-Prot
Match: Q9C5E5 (Diacylglycerol kinase 5 OS=Arabidopsis thaliana OX=3702 GN=DGK5 PE=2 SV=1)

HSP 1 Score: 210.7 bits (535), Expect = 3.3e-53
Identity = 128/387 (33.07%), Postives = 187/387 (48.32%), Query Frame = 0


Query: 91  PESPMVVFINPRSGGRHGPLLKERLQMLISEEQVFDLTDVKPHEFVRYGLRCLELLAGLG 150
           P SP++VFIN +SGG+ G  L    + L++  QVFDL    P + +R     LE L    
Sbjct: 37  PASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLK--D 96

Query: 151 DACAKEIREKMRVMVAGGDGTVGWVLGCLLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGW 210
           D  A++IREK++++VAGGDGT GW+LG + DL+ S     PP+  +PLGTGN+L   FGW
Sbjct: 97  DDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLS---HPPPIATVPLGTGNNLPFAFGW 156

Query: 211 GGSFPFA--------------------WNWHVTLATPSTK---------IVKLPHCMKPT 270
           G   P                       NWH+ +   + K          ++LPH     
Sbjct: 157 GKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPH----- 216

Query: 271 EVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVAYGFHQLRDEKPYLAQGPISNK 330
            +HA       +E   E  +   G F+NYFS+GMDAQ++Y FH  R   P   +  + N+
Sbjct: 217 SLHAFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQLVNQ 276

Query: 331 LIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNCSDWEEVLIPSSVRSLVALNLN 390
             Y    C+QGW F   +  P+ R +  + ++ +   N   W+++ IP S+RS+V LNL 
Sbjct: 277 STYVKLGCTQGW-FCASLFHPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRSIVCLNLP 336

Query: 391 NYGSGRHPWG----------NLTPEYME-----------------------------RAA 410
           ++  G +PWG           LTP +++                             +A 
Sbjct: 337 SFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQAN 396

[Close]

BLAST of Carg16449 vs. ExPASy Swiss-Prot
Match: Q7XQT2 (Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 209.9 bits (533), Expect = 5.7e-53
Identity = 132/398 (33.17%), Postives = 196/398 (49.25%), Query Frame = 0


Query: 88  DGPPESPMVVFINPRSGGRHGPLLKERLQMLISEEQVFDLTDVKPHEFVRYGLRC-LELL 147
           D  P  P+VVFIN RSGG+ G  L +  + L+++ QVFDL++  P E V + L C  E L
Sbjct: 39  DQAPCCPVVVFINSRSGGQLGSSLIKTYRELLNKAQVFDLSEEAP-EKVLHRLYCNFEKL 98

Query: 148 AGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLLDLEKSGRLPVPPVGIIPLGTGNDLSR 207
              GD  A +I+  +R++VAGGDGT  W+LG + DL+ S     PP+  +PLGTGN+L  
Sbjct: 99  KSNGDPIAFQIQSNLRLIVAGGDGTASWLLGVVSDLKLS---HPPPIATVPLGTGNNLPF 158

Query: 208 TFGWGGSFPFA--------------------WNWHVTLAT------PSTKI--VKLPHCM 267
           +FGWG   P                       +WH+ +        P   I  ++LPH  
Sbjct: 159 SFGWGKKNPTTDQEAVKSFLGQVKKAREMNIDSWHIIMRMRAPQEGPCEPIAPLELPH-- 218

Query: 268 KPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVAYGFHQLRDEKPYLAQGPI 327
               +HA       +    E  + + G F+NYFS+GMDAQV+Y FH  R   P   +  +
Sbjct: 219 ---SLHAFHRVSGSDSLNMEGYHTYRGGFWNYFSMGMDAQVSYEFHSERKRNPEKFKNQL 278

Query: 328 SNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNCSDWEEVLIPSSVRSLVAL 387
           +N+  Y+     QGW F   ++ PS R +  + ++ + K     WEE+ IP S+RS+V L
Sbjct: 279 TNQSTYAKLGLKQGW-FAASLTHPSSRNIAQLAKVRIMKRPGGQWEELKIPRSIRSIVCL 338

Query: 388 NLNNYGSGRHPWG----------NLTPEYME----------------------------- 418
           NL ++  G +PWG          +LT  +++                             
Sbjct: 339 NLPSFSGGLNPWGTPGTRKVQDRDLTAPFVDDGLIEVVGFRDAWHGLVLLAPNGHGTRLA 398

[Close]

BLAST of Carg16449 vs. ExPASy TrEMBL
Match: A0A6J1FBI4 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111444111 PE=3 SV=1)

HSP 1 Score: 874.0 bits (2257), Expect = 2.6e-250
Identity = 431/491 (87.78%), Postives = 432/491 (87.98%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60
           MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR
Sbjct: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60

Query: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120
           LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS
Sbjct: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW--------------------NW 240
           DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW                    +W
Sbjct: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRRLDSW 240

Query: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300
           HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA
Sbjct: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360
           YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC
Sbjct: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360

Query: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER-------------------- 420
           SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER                    
Sbjct: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMERRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 -------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 433
                              AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR
Sbjct: 421 GWHASLVMGEVISAKHIVQAAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 480

[Close]

BLAST of Carg16449 vs. ExPASy TrEMBL
Match: A0A6J1I0M6 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111468641 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.4e-248
Identity = 429/491 (87.37%), Postives = 430/491 (87.58%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60
           MDSPTSGGEGDSTTRIAAQSSVVDS KG GFTGIKIDKDELRKQLRMPQYLRYAMRDSIR
Sbjct: 1   MDSPTSGGEGDSTTRIAAQSSVVDSLKGFGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60

Query: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120
           LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS
Sbjct: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW--------------------NW 240
           DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW                    +W
Sbjct: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAWKSAIKKSLDRATTGQIRRLDSW 240

Query: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300
           HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA
Sbjct: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360
           YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC
Sbjct: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360

Query: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER-------------------- 420
           SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER                    
Sbjct: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMERRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 -------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 433
                              AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR
Sbjct: 421 GWHASLVMGEVISAKHIVQAAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 480

[Close]

BLAST of Carg16449 vs. ExPASy TrEMBL
Match: A0A6J1EPG0 (diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111436187 PE=3 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 8.7e-214
Identity = 369/491 (75.15%), Postives = 401/491 (81.67%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60
           MDSPTS G GDSTTRI A+SS+++SFKGCG TG++IDK++LRKQLRMPQYLR+AMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120
           LQDPGAGE+ LPGA SISGS    + +DGPPESPMVVFINPRSGGRHG LLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPRSGGRHGRLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKE REKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW--------------------NW 240
           +LEK GRLPVPPVG+IPLGTGNDLSRTFGWGGSFPFAW                    +W
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300
            V L++PS + + LPHCMKPTE HALDESLEIEE+LPEKA  HEGVFYNYFSIGMDAQVA
Sbjct: 241 QVFLSSPSGENMDLPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360
           YGFH LR+EKPYLAQGPI+NKLIYSGYSC QGWFFTPC SDPSLRGLK+ILRMHVKK+NC
Sbjct: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKINC 360

Query: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER-------------------- 420
           SDWE + IPSSVRSLVALNLNNYGSGRHPWGNL+PEYME+                    
Sbjct: 361 SDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 -------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 433
                              AAAIRFELRGGEW++AFLQMDGEPWKQPM+ E  T+VEIKR
Sbjct: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEIKR 480

[Close]

BLAST of Carg16449 vs. ExPASy TrEMBL
Match: A0A6J1KDS8 (diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111492334 PE=3 SV=1)

HSP 1 Score: 750.4 bits (1936), Expect = 4.3e-213
Identity = 367/491 (74.75%), Postives = 401/491 (81.67%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60
           MDSPTS G GDSTTRI A+SS+++SFKGCG TG++IDK++LRKQLRMPQYLR+AMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIPARSSMIESFKGCGLTGMRIDKEKLRKQLRMPQYLRFAMRDSIR 60

Query: 61  LQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQMLIS 120
           LQDPGAGE+ LPGA SISGS    + +DGPPESPMVVFINP+SGGRHG LLKERLQMLIS
Sbjct: 61  LQDPGAGESRLPGASSISGSPFAQDTDDGPPESPMVVFINPQSGGRHGRLLKERLQMLIS 120

Query: 121 EEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGCLL 180
           EEQVFD+TDVKPHEFVRYGLRCLELLAGLGDACAKE REKMRVMVAGGDGTVGWVLGCLL
Sbjct: 121 EEQVFDITDVKPHEFVRYGLRCLELLAGLGDACAKETREKMRVMVAGGDGTVGWVLGCLL 180

Query: 181 DLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW--------------------NW 240
           +LEK GRLPVPPVG+IPLGTGNDLSRTFGWGGSFPFAW                    +W
Sbjct: 181 ELEKKGRLPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGKIRRLDSW 240

Query: 241 HVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVA 300
            V L++PS + + LPHCMKPTE HALDESLEIEE+LPEKA  HEGVFYNYFSIGMDAQVA
Sbjct: 241 QVFLSSPSGENMDLPHCMKPTEDHALDESLEIEEALPEKATCHEGVFYNYFSIGMDAQVA 300

Query: 301 YGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNC 360
           YGFH LR+EKPYLAQGPI+NKLIYSGYSC QGWFFTPC SDPSLRGLK+ILRMHVKK+NC
Sbjct: 301 YGFHHLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPSLRGLKNILRMHVKKINC 360

Query: 361 SDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER-------------------- 420
           SDWE + IPSSVRSLVALNLNNYGSGRHPWGNL+PEYME+                    
Sbjct: 361 SDWEHISIPSSVRSLVALNLNNYGSGRHPWGNLSPEYMEKRGFVEAHVDDGLLEVFGLKQ 420

Query: 421 -------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEIKR 433
                              AAAIRFELRGGEW++AFLQMDGEPWKQPM+ E  T+VEIKR
Sbjct: 421 GWHASLVMGELISAKHIVQAAAIRFELRGGEWRNAFLQMDGEPWKQPMNDEFSTFVEIKR 480

[Close]

BLAST of Carg16449 vs. ExPASy TrEMBL
Match: A0A1S3BRW0 (diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103493013 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.9e-208
Identity = 363/493 (73.63%), Postives = 398/493 (80.73%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKGCGFTGIKIDKDELRKQLRMPQYLRYAMRDSIR 60
           MDSPTS G GDSTTRIAA+SS+++SFKGCG TG++IDK++LRK+L+MPQYLR+AMRDSIR
Sbjct: 1   MDSPTSRGGGDSTTRIAARSSMIESFKGCGLTGLRIDKEDLRKKLQMPQYLRFAMRDSIR 60

Query: 61  LQDPGAGETWLPGAR--SISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERLQML 120
           LQDP AGE+ LPG R  S+S S+ VPE  + PPESPMVVFINPRSGGRHG LLK+RLQML
Sbjct: 61  LQDPSAGESRLPGTRLDSLSASTFVPESTEEPPESPMVVFINPRSGGRHGRLLKDRLQML 120

Query: 121 ISEEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWVLGC 180
           ISEEQVFDLTDVKPHEFVRYGL CLELLA LGDACAK++R+K+RVMVAGGDGTVGWVLGC
Sbjct: 121 ISEEQVFDLTDVKPHEFVRYGLGCLELLAELGDACAKDVRQKIRVMVAGGDGTVGWVLGC 180

Query: 181 LLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW-------------------- 240
           LL+LEK  R PVPPVG+IPLGTGNDLSRTFGWGGSFPFAW                    
Sbjct: 181 LLELEKKDRRPVPPVGVIPLGTGNDLSRTFGWGGSFPFAWKSAIKRSLDRATTGQIRKLD 240

Query: 241 NWHVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQ 300
           +WHV L+TPS + V+LPHCMKPTE  ALDESLEIEE+L EKA  HEGVFYNYFSIGMDAQ
Sbjct: 241 SWHVFLSTPSGENVQLPHCMKPTEELALDESLEIEEALSEKASCHEGVFYNYFSIGMDAQ 300

Query: 301 VAYGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKV 360
           VAYGFH LR+EKPYLAQGPI+NKLIYSGYSC QGWFFTPC SDP LRGLK+ILRMHVKKV
Sbjct: 301 VAYGFHNLRNEKPYLAQGPIANKLIYSGYSCGQGWFFTPCSSDPGLRGLKNILRMHVKKV 360

Query: 361 NCSDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER------------------ 420
           NCSDWE++LIPSSVRSLVALNLNNYGSGRHPWGNLTPEYME+                  
Sbjct: 361 NCSDWEQILIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMEKRGFVEAQVDDGLLEVFGL 420

Query: 421 ---------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTYVEI 433
                                AAAIRFELRGGEWK AFLQMDGEPWKQPMS E  T+VEI
Sbjct: 421 KQGWHASLVMGELISAKHIVQAAAIRFELRGGEWKDAFLQMDGEPWKQPMSNEFSTFVEI 480

[Close]

BLAST of Carg16449 vs. TAIR 10
Match: AT4G30340.1 (diacylglycerol kinase 7 )

HSP 1 Score: 561.2 bits (1445), Expect = 7.1e-160
Identity = 277/500 (55.40%), Postives = 348/500 (69.60%), Query Frame = 0


Query: 2   DSPTSGGEGDSTTRIAAQSSV----VDSFKGCGFTG---IKIDKDELRKQLRMPQYLRYA 61
           ++P S GE  +T  +AA+ S       + +GCGF     + IDK+ELR +L MP+YLR A
Sbjct: 3   ETPRSVGEASTTNFVAARPSAKTDDAVTMRGCGFANLALVGIDKEELRGRLAMPEYLRIA 62

Query: 62  MRDSIRLQDPG--AGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLK 121
           MRD I+ +D    +    LPG  +           D  P +PMVVFINP+SGGRHGP+LK
Sbjct: 63  MRDCIKRKDSTEISDHLLLPGGAAA----------DMAPHAPMVVFINPKSGGRHGPVLK 122

Query: 122 ERLQMLISEEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTV 181
           ERLQ L++EEQVFDLT+VKPHEFVRYGL CL+ LA  GD CA+E REK+R+MVAGGDGTV
Sbjct: 123 ERLQQLMTEEQVFDLTEVKPHEFVRYGLGCLDTLAAKGDECARECREKIRIMVAGGDGTV 182

Query: 182 GWVLGCLLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW-------------- 241
           GWVLGCL +L K G+  +PPVG+IPLGTGNDLSR+F WGGSFPFAW              
Sbjct: 183 GWVLGCLGELHKDGKSHIPPVGVIPLGTGNDLSRSFSWGGSFPFAWRSAMKRTLHRATLG 242

Query: 242 ------NWHVTLATPSTKIVKLPHCMKPT-EVHALDESLEIEESLPEKAYFHEGVFYNYF 301
                 +W + ++ PS ++V  P+ +KPT E  ALD++L+ +  +P KA  +EGVFYNYF
Sbjct: 243 SIARLDSWKIVVSMPSGEVVDPPYSLKPTIEETALDQALDADGDVPPKAKSYEGVFYNYF 302

Query: 302 SIGMDAQVAYGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHIL 361
           SIGMDAQVAYGFH LR+EKPYLAQGP++NK+IYS YSC+QGWF TPC+++P+LRGL++I+
Sbjct: 303 SIGMDAQVAYGFHHLRNEKPYLAQGPVTNKIIYSSYSCTQGWFCTPCVNNPALRGLRNIM 362

Query: 362 RMHVKKVNCSDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER----------- 421
           ++H+KK NCS+WEE+ +P SVRS+V LNL NYGSGRHPWGNL P+Y+E+           
Sbjct: 363 KIHIKKANCSEWEEIHVPKSVRSIVVLNLYNYGSGRHPWGNLRPKYLEKRGFVEAHCDDG 422

Query: 422 ----------------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRE 433
                                       AAAIRFELRGG+WK+AFLQMDGEPWKQPM  +
Sbjct: 423 LIEIFGLKQGWHASFVMAEIISAKHIAQAAAIRFELRGGDWKNAFLQMDGEPWKQPMKSD 482

[Close]

BLAST of Carg16449 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 554.3 bits (1427), Expect = 8.7e-158
Identity = 273/496 (55.04%), Postives = 338/496 (68.15%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDS--FKGCGFTG---IKIDKDELRKQLRMPQYLRYAM 60
           MDSP S  +      +A++ S  DS   +GCG      + +DK ELR++L MP+YLR AM
Sbjct: 1   MDSPVSKTDASKEKFVASRPSTADSKTMRGCGLANLAWVGVDKVELRQRLMMPEYLRLAM 60

Query: 61  RDSIRLQDPGAGETWLPGARSISGSSVVPEINDGPPESPMVVFINPRSGGRHGPLLKERL 120
           RD I+ +D  A    +P    + G +V     D  P +PMVVFINP SGGRHGP+LKERL
Sbjct: 61  RDCIKRKDSSA----IPDHLLLPGGAVA----DMAPHAPMVVFINPNSGGRHGPVLKERL 120

Query: 121 QMLISEEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTVGWV 180
           Q L+SEEQVFDLT+VKPHEFVRYGL CLE +A  GD CAKE R ++R+MVAGGDGTVGWV
Sbjct: 121 QQLMSEEQVFDLTEVKPHEFVRYGLGCLEKVAAEGDECAKECRARLRIMVAGGDGTVGWV 180

Query: 181 LGCLLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW----------------- 240
           LGCL +L K G+  +PPVG+IPLGTGNDLSR+FGWGGSFPFAW                 
Sbjct: 181 LGCLGELNKDGKSQIPPVGVIPLGTGNDLSRSFGWGGSFPFAWRSAVKRTLHRASMGPVA 240

Query: 241 ---NWHVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFSIGM 300
              +W + ++ PS ++V  P+ +KP E + LD+ L+     P  A  +EGVFYNY SIGM
Sbjct: 241 RLDSWKILVSMPSGEVVDPPYSLKPAEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGM 300

Query: 301 DAQVAYGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHV 360
           DAQVAYGFH LR+ KPYLAQGPISNK+IYS + CSQGWF TPC++DP LRGL++I+++H+
Sbjct: 301 DAQVAYGFHHLRNTKPYLAQGPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHI 360

Query: 361 KKVNCSDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER--------------- 420
           KKVNCS WEE+ +P +VRS+VALNL++YGSG HPWGNL P+Y+E+               
Sbjct: 361 KKVNCSQWEEIAVPKNVRSIVALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEI 420

Query: 421 ------------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSRECLTY 433
                                   AAA+RFELRGG+W+ AFLQMDGEPWKQPMS E  T+
Sbjct: 421 FGFKQGWHASFVMAELISAKHIAQAAAVRFELRGGDWRDAFLQMDGEPWKQPMSTEYSTF 480

[Close]

BLAST of Carg16449 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 525.0 bits (1351), Expect = 5.6e-149
Identity = 264/499 (52.91%), Postives = 336/499 (67.33%), Query Frame = 0


Query: 1   MDSPTSGGEGDSTTRIAAQSSVVDSFKG------CGFTGIKIDKDELRKQLRMPQYLRYA 60
           M+SP+ G     T R+  + S +DSF             I++ K ELR+++ +PQYLR A
Sbjct: 1   MESPSIG--DSLTARMIPRHSSLDSFGAMKVSLLVNLASIRVSKAELRQRVMLPQYLRIA 60

Query: 61  MRDSIRLQDPGAGETWLPGARSISGSSVVP--EINDGPPESPMVVFINPRSGGRHGPLLK 120
           +RD I  +D              + SSV P  E N   PE P++VF+NP+SGGR GPL+K
Sbjct: 61  IRDCILRKDDSFD----------ASSSVAPPLENNALTPEVPLMVFVNPKSGGRQGPLIK 120

Query: 121 ERLQMLISEEQVFDLTDVKPHEFVRYGLRCLELLAGLGDACAKEIREKMRVMVAGGDGTV 180
           ERLQ LISEEQV+DLT+VKP+EF+RYGL CLE  A  GD CAKEIREKMR++VAGGDGTV
Sbjct: 121 ERLQNLISEEQVYDLTEVKPNEFIRYGLGCLEAFASRGDECAKEIREKMRIVVAGGDGTV 180

Query: 181 GWVLGCLLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGWGGSFPFAW-------------- 240
           GWVLGCL +L    RLPVPPV I+PLGTGNDLSR+FGWGGSFPFAW              
Sbjct: 181 GWVLGCLGELNLQNRLPVPPVSIMPLGTGNDLSRSFGWGGSFPFAWKSAIKRTLHRASVA 240

Query: 241 ------NWHVTLATPSTKIVKLPHCMKPTEVHALDESLEIEESLPEKAYFHEGVFYNYFS 300
                 +W++ +  PS +IV  P+ +K T+   +D++LEIE  +P     +EGVFYNYFS
Sbjct: 241 PISRLDSWNILITMPSGEIVDPPYSLKATQECYIDQNLEIEGEIPPSTNGYEGVFYNYFS 300

Query: 301 IGMDAQVAYGFHQLRDEKPYLAQGPISNKLIYSGYSCSQGWFFTPCISDPSLRGLKHILR 360
           IGMDAQVAYGFH LR+EKPYLA GPI+NK+IYSGY CSQGWF T CI+DP LRGLK+I+ 
Sbjct: 301 IGMDAQVAYGFHHLRNEKPYLANGPIANKIIYSGYGCSQGWFLTHCINDPGLRGLKNIMT 360

Query: 361 MHVKKVNCSDWEEVLIPSSVRSLVALNLNNYGSGRHPWGNLTPEYMER------------ 420
           +H+KK++ S+WE+V +P SVR++VALNL++YGSGR+PWGNL  +Y+E+            
Sbjct: 361 LHIKKLDSSEWEKVPVPKSVRAVVALNLHSYGSGRNPWGNLKQDYLEKRGFVEAQADDGL 420

Query: 421 ---------------------------AAAIRFELRGGEWKHAFLQMDGEPWKQPMSREC 433
                                      AAAIR E+RGG+WK AF+QMDGEPWKQPM+R+ 
Sbjct: 421 LEIFGLKQGWHASFVMVELISAKHIAQAAAIRLEIRGGDWKDAFMQMDGEPWKQPMTRDY 480

[Close]

BLAST of Carg16449 vs. TAIR 10
Match: AT2G20900.1 (diacylglycerol kinase 5 )

HSP 1 Score: 210.7 bits (535), Expect = 2.4e-54
Identity = 128/387 (33.07%), Postives = 187/387 (48.32%), Query Frame = 0


Query: 91  PESPMVVFINPRSGGRHGPLLKERLQMLISEEQVFDLTDVKPHEFVRYGLRCLELLAGLG 150
           P SP++VFIN +SGG+ G  L    + L++  QVFDL    P + +R     LE L    
Sbjct: 37  PASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLK--D 96

Query: 151 DACAKEIREKMRVMVAGGDGTVGWVLGCLLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGW 210
           D  A++IREK++++VAGGDGT GW+LG + DL+ S     PP+  +PLGTGN+L   FGW
Sbjct: 97  DDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLS---HPPPIATVPLGTGNNLPFAFGW 156

Query: 211 GGSFPFA--------------------WNWHVTLATPSTK---------IVKLPHCMKPT 270
           G   P                       NWH+ +   + K          ++LPH     
Sbjct: 157 GKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPH----- 216

Query: 271 EVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVAYGFHQLRDEKPYLAQGPISNK 330
            +HA       +E   E  +   G F+NYFS+GMDAQ++Y FH  R   P   +  + N+
Sbjct: 217 SLHAFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQLVNQ 276

Query: 331 LIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNCSDWEEVLIPSSVRSLVALNLN 390
             Y    C+QGW F   +  P+ R +  + ++ +   N   W+++ IP S+RS+V LNL 
Sbjct: 277 STYVKLGCTQGW-FCASLFHPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRSIVCLNLP 336

Query: 391 NYGSGRHPWG----------NLTPEYME-----------------------------RAA 410
           ++  G +PWG           LTP +++                             +A 
Sbjct: 337 SFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQAN 396

[Close]

BLAST of Carg16449 vs. TAIR 10
Match: AT2G20900.3 (diacylglycerol kinase 5 )

HSP 1 Score: 210.7 bits (535), Expect = 2.4e-54
Identity = 128/387 (33.07%), Postives = 187/387 (48.32%), Query Frame = 0


Query: 91  PESPMVVFINPRSGGRHGPLLKERLQMLISEEQVFDLTDVKPHEFVRYGLRCLELLAGLG 150
           P SP++VFIN +SGG+ G  L    + L++  QVFDL    P + +R     LE L    
Sbjct: 37  PASPVLVFINSKSGGQLGGELILTYRSLLNHNQVFDLDQETPDKVLRRIYLNLERLK--D 96

Query: 151 DACAKEIREKMRVMVAGGDGTVGWVLGCLLDLEKSGRLPVPPVGIIPLGTGNDLSRTFGW 210
           D  A++IREK++++VAGGDGT GW+LG + DL+ S     PP+  +PLGTGN+L   FGW
Sbjct: 97  DDFARQIREKLKIIVAGGDGTAGWLLGVVCDLKLS---HPPPIATVPLGTGNNLPFAFGW 156

Query: 211 GGSFPFA--------------------WNWHVTLATPSTK---------IVKLPHCMKPT 270
           G   P                       NWH+ +   + K          ++LPH     
Sbjct: 157 GKKNPGTDRTAVESFLEQVLKAKVMKIDNWHILMRMKTPKEGGSCDPVAPLELPH----- 216

Query: 271 EVHALDESLEIEESLPEKAYFHEGVFYNYFSIGMDAQVAYGFHQLRDEKPYLAQGPISNK 330
            +HA       +E   E  +   G F+NYFS+GMDAQ++Y FH  R   P   +  + N+
Sbjct: 217 SLHAFHRVSPTDELNKEGCHTFRGGFWNYFSLGMDAQISYAFHSERKLHPEKFKNQLVNQ 276

Query: 331 LIYSGYSCSQGWFFTPCISDPSLRGLKHILRMHVKKVNCSDWEEVLIPSSVRSLVALNLN 390
             Y    C+QGW F   +  P+ R +  + ++ +   N   W+++ IP S+RS+V LNL 
Sbjct: 277 STYVKLGCTQGW-FCASLFHPASRNIAQLAKVKIATRN-GQWQDLHIPHSIRSIVCLNLP 336

Query: 391 NYGSGRHPWG----------NLTPEYME-----------------------------RAA 410
           ++  G +PWG           LTP +++                             +A 
Sbjct: 337 SFSGGLNPWGTPNPRKQRDRGLTPPFVDDGLIEVVGFRNAWHGLVLLAPNGHGTRLAQAN 396

The following BLAST results are available for this feature:

BLAST of Carg16449 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020845.1	5.7e-260	100.00	Diacylglycerol kinase 7, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022937846.1	5.3e-250	87.78	diacylglycerol kinase 7-like [Cucurbita moschata]	[more]
XP_023538307.1	1.2e-249	87.58	diacylglycerol kinase 7-like [Cucurbita pepo subsp. pepo]	[more]
XP_022969690.1	5.0e-248	87.37	diacylglycerol kinase 7-like [Cucurbita maxima]	[more]
XP_038890957.1	1.9e-215	75.56	diacylglycerol kinase 7-like [Benincasa hispida]	[more]

BLAST of Carg16449 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JQ95	1.0e-158	55.40	Diacylglycerol kinase 7 OS=Arabidopsis thaliana OX=3702 GN=DGK7 PE=1 SV=1	[more]
Q8VZG1	1.2e-156	55.04	Diacylglycerol kinase 3 OS=Arabidopsis thaliana OX=3702 GN=DGK3 PE=1 SV=1	[more]
Q1PDI2	7.9e-148	52.91	Diacylglycerol kinase 4 OS=Arabidopsis thaliana OX=3702 GN=DGK4 PE=1 SV=1	[more]
Q9C5E5	3.3e-53	33.07	Diacylglycerol kinase 5 OS=Arabidopsis thaliana OX=3702 GN=DGK5 PE=2 SV=1	[more]
Q7XQT2	5.7e-53	33.17	Diacylglycerol kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DGK1 PE=1 SV...	[more]

BLAST of Carg16449 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FBI4	2.6e-250	87.78	diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111444111 PE=3 SV=1	[more]
A0A6J1I0M6	2.4e-248	87.37	diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111468641 PE=3 SV=1	[more]
A0A6J1EPG0	8.7e-214	75.15	diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111436187 PE=3 SV=1	[more]
A0A6J1KDS8	4.3e-213	74.75	diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111492334 PE=3 SV=1	[more]
A0A1S3BRW0	1.9e-208	73.63	diacylglycerol kinase OS=Cucumis melo OX=3656 GN=LOC103493013 PE=3 SV=1	[more]

BLAST of Carg16449 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30340.1	7.1e-160	55.40	diacylglycerol kinase 7	[more]
AT2G18730.1	8.7e-158	55.04	diacylglycerol kinase 3	[more]
AT5G57690.1	5.6e-149	52.91	diacylglycerol kinase 4	[more]
AT2G20900.1	2.4e-54	33.07	diacylglycerol kinase 5	[more]
AT2G20900.3	2.4e-54	33.07	diacylglycerol kinase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000756	Diacylglycerol kinase, accessory domain	SMART	SM00045	dagk_c4b_2	coord: 266..404 e-value: 2.0E-4 score: -28.1
IPR000756	Diacylglycerol kinase, accessory domain	PFAM	PF00609	DAGK_acc	coord: 266..376 e-value: 1.6E-23 score: 83.8
IPR001206	Diacylglycerol kinase, catalytic domain	SMART	SM00046	dagk_c4a_7	coord: 94..247 e-value: 1.7E-21 score: 87.4
IPR001206	Diacylglycerol kinase, catalytic domain	PFAM	PF00781	DAGK_cat	coord: 94..219 e-value: 7.1E-28 score: 96.7
IPR001206	Diacylglycerol kinase, catalytic domain	PROSITE	PS50146	DAGK	coord: 90..211 score: 25.827215
IPR017438	Inorganic polyphosphate/ATP-NAD kinase, N-terminal	GENE3D	3.40.50.10330		coord: 92..232 e-value: 5.2E-20 score: 73.3
None	No IPR available	PANTHER	PTHR11255:SF96	DIACYLGLYCEROL KINASE	coord: 10..219 coord: 220..379 coord: 379..431
IPR037607	Diacylglycerol kinase	PANTHER	PTHR11255	DIACYLGLYCEROL KINASE	coord: 220..379 coord: 379..431
IPR037607	Diacylglycerol kinase	PANTHER	PTHR11255	DIACYLGLYCEROL KINASE	coord: 10..219
IPR016064	NAD kinase/diacylglycerol kinase-like domain superfamily	SUPERFAMILY	111331	NAD kinase/diacylglycerol kinase-like	coord: 94..423

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg16449-RA	Carg16449-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006952	defense response
biological_process	GO:0016310	phosphorylation
biological_process	GO:0007205	protein kinase C-activating G protein-coupled receptor signaling pathway
biological_process	GO:0007165	signal transduction
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004143	diacylglycerol kinase activity
molecular_function	GO:0003951	NAD+ kinase activity
molecular_function	GO:0016301	kinase activity