Carg16340 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg16340
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAmino acid permease family protein
LocationCarg_Chr19: 6040775 .. 6043386 (+)
RNA-Seq ExpressionCarg16340
SyntenyCarg16340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CATTGGCCAAGAACAGCACAGATTTAGGTTCATTTCCCTGCAATTTCCAGCGGATCTCGCTCATATTCCCAATTCCGTTTGATTTTTGCGGTGTTTCTTCATCTCTGAGAACAGCCCTGTCCTGTCCATGGGCGACGAGAGGGCTAATATTACTCCAAAACTAACGATTCTTCCTCTCATCGCTTTGATTTTCTACGACGTTTCAGGAGGACCCTTCGGAGTGGAGGATTCCGTGAGCACTGGCGGTGGTCCGCTTCTAGCTTTGCTAGGTTTCTTACTGTTTCCCTTTATTTGGAGTATTCCGGAGGCGTTGGTCACGGCGGAGCTCGCCACAAGCTTCCCCCAAAACGGCGGATATGTGGTCTGGATTTCGGCAGCTTTTGGCCCGTTTTGGGGGTTTCAAGAGGGCTTCTGGAAATGGTTCAGTGGGGTAATGGATAATGCTCTGTATCCTGTACTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAGCCATATATTTGCTCGAATTCCAGCTCTGTTAGGAATCACTGCTTCTTTAACTTACTTGAACTATCGTGGTCTGCATATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTGTCCTTTTGTAGTGATGACCATTCTTTCAATTCCCAGAATAAGGCCTAGGAAGTGGCTAGTTGTAGATTATAGTATGGTGAATTGGAGGGGCTATTTCAATAGTATGTTTTGGAACCTGAACTATTGGGATAAGGCAAGTACTTTAGCTGGGGAGGTTGAGAATCCTAGCAAGACCTTCCCCAAGGCTATGTTTGGGGCTGTGGTTTTGGTGGTTTGTTCTTATTTGGTTCCTCTTTTGGCTGGGACTGGTGCGTTGGAGACAGATTCAAGTGAGTGGAGTGATGGGTATTTTGCTGAGGTTGGGGCGTTGATTGGTGGGGCGTGGCTGAAGTGGTGGATTCAAGCGGCTGCTGCTATGTCTAACATGGGGTTGTTTGAGGCTGAAATGAGTAGTGATGCATATCAACTGCTTGGGATGAGTGAGATGGGCATGATTCCATCTGTGTTTGCTTCAAGGTTGGTGGTTCTGTTTAGTTTCCTTCTCATTGTTCTGTATTGTTTGCAACTTGAGTAGCAATAGTCTTGCCTTATGTACCCACAAATGAGATTTGTAGCTCGAATTGGTAGTAGCGAGTCATATTAGCAATCCTTTTGAGGGTGGGAGGTCGAAGGTTTGTATCACCATGGAGGTAAAAACATAGGTAGCTCAAATGGGTCGGGATAACAAGATGTGTTTTAGTAGTAAGTTGACATTGACATAACTTGTAATAGTTCAAACCCACGGTCAGTAGATATTGTCCTTTTTGGGCTTTCCCTTTCATGCATCCTCTCAAAGTTTTTAAAACGTGTCTATTAGGGAGAGGTTTCTACACCCTTATAAACAGTGCTTCGTTCTCCTTTCCAATTGACGTGGGATCTCACCTGATGTACCCACAAATGAGATTTGTAGCTCGAATCAGTGGTAGTGAGTCATAATAGCAATCCTTTGAGGGGGGAGGTCGAAGGTTTATATCACCATGGACGTAAAACCATAGGTAGCTCAAATGGGTTGGGATAATAATATGCATTTTATTAGTAAGTTGACATTGACATAACTTGTAACAGTCCAAGCCCACTGCTAGCAGATATTGTCCTCTTTGGGTTTCACTTTCGAGCTTCCCCTCATTGTTTTTAAAACGCGTTTGCTAGAGAAAGGTTTCCACACCCTTATAAAAAAAATGTTTCGTTCTCCTCTCCAACTGATGTGGGATCTCACAATCCACCTCCTTCGAGGCCTAGCGTCCTCGCTGACACTCGTTCTACTATGGAATCTCCATAACCCCTAAGACTATAAGACTCGTAGGAGTTCTTTAAACTTCCAAGATGAGTTTAGGTAGCTCAAAACTTTATGCCAAACATTTCCAAAACTCCTTTTCTCCTATCATCATACCCAAACGCAAGTGAACTTCCTCCTTAAACTCTTCCAATGTTTTGATCCATTTGATTTACAGGCACATTGTGGATGCAACAGCTGATCTTATTGAACTACTGTCTCTCATTCACTTTGCAGATCAAAATATGGGACACCCACCTTCAGCATTCTGTGCTCTGCCATTGGGGTTATCTTCCTCTCATGGATGAGTTTCCAAGAAATACTCGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTTTGGAGCTTGCTGCCTTTATAAAACTTAGAATAAAGAAGCCAGACCTCCACAGACCTTACAAAGTTCCCTTGCAAACATTTGGTGCCATAATGCTTTGCTTGCCACCTGCAGCTTTGCTTGTTCTTGTCATGTGTTTAGCTTCTGCACAAACATTCTTAATCAGTGGGGTTATAATTGCTGTAGGGTTTCTTCTATACCCTTCTCTTTCACAAGCGAAGAACAGAGGATGGGTCAAATTTATTTCAGAACAGCAAGGAGATACTCTTCCAGATGTGGAAGACTGCCTGGTCGAGCCGCAACCACGGCAAGAAGTTCTCGATGAGTCAGTGATTCGGCTTCTTTCAGAATCTCCTTCGTCTCCGAATATAGCCAACCAATAA

mRNA sequence

CATTGGCCAAGAACAGCACAGATTTAGGTTCATTTCCCTGCAATTTCCAGCGGATCTCGCTCATATTCCCAATTCCGTTTGATTTTTGCGGTGTTTCTTCATCTCTGAGAACAGCCCTGTCCTGTCCATGGGCGACGAGAGGGCTAATATTACTCCAAAACTAACGATTCTTCCTCTCATCGCTTTGATTTTCTACGACGTTTCAGGAGGACCCTTCGGAGTGGAGGATTCCGTGAGCACTGGCGGTGGTCCGCTTCTAGCTTTGCTAGGTTTCTTACTGTTTCCCTTTATTTGGAGTATTCCGGAGGCGTTGGTCACGGCGGAGCTCGCCACAAGCTTCCCCCAAAACGGCGGATATGTGGTCTGGATTTCGGCAGCTTTTGGCCCGTTTTGGGGGTTTCAAGAGGGCTTCTGGAAATGGTTCAGTGGGGTAATGGATAATGCTCTGTATCCTGTACTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAGCCATATATTTGCTCGAATTCCAGCTCTGTTAGGAATCACTGCTTCTTTAACTTACTTGAACTATCGTGGTCTGCATATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTGTCCTTTTGTAGTGATGACCATTCTTTCAATTCCCAGAATAAGGCCTAGGAAGTGGCTAGTTGTAGATTATAGTATGGTGAATTGGAGGGGCTATTTCAATAGTATGTTTTGGAACCTGAACTATTGGGATAAGGCAAGTACTTTAGCTGGGGAGGTTGAGAATCCTAGCAAGACCTTCCCCAAGGCTATGTTTGGGGCTGTGGTTTTGGTGGTTTGTTCTTATTTGGTTCCTCTTTTGGCTGGGACTGGTGCGTTGGAGACAGATTCAAGTGAGTGGAGTGATGGGTATTTTGCTGAGGTTGGGGCGTTGATTGGTGGGGCGTGGCTGAAGTGGTGGATTCAAGCGGCTGCTGCTATGTCTAACATGGGGTTGTTTGAGGCTGAAATGAGTAGTGATGCATATCAACTGCTTGGGATGAGTGAGATGGGCATGATTCCATCTGTGTTTGCTTCAAGATCAAAATATGGGACACCCACCTTCAGCATTCTGTGCTCTGCCATTGGGGTTATCTTCCTCTCATGGATGAGTTTCCAAGAAATACTCGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTTTGGAGCTTGCTGCCTTTATAAAACTTAGAATAAAGAAGCCAGACCTCCACAGACCTTACAAAGTTCCCTTGCAAACATTTGGTGCCATAATGCTTTGCTTGCCACCTGCAGCTTTGCTTGTTCTTGTCATGTGTTTAGCTTCTGCACAAACATTCTTAATCAGTGGGGTTATAATTGCTGTAGGGTTTCTTCTATACCCTTCTCTTTCACAAGCGAAGAACAGAGGATGGGTCAAATTTATTTCAGAACAGCAAGGAGATACTCTTCCAGATGTGGAAGACTGCCTGGTCGAGCCGCAACCACGGCAAGAAGTTCTCGATGAGTCAGTGATTCGGCTTCTTTCAGAATCTCCTTCGTCTCCGAATATAGCCAACCAATAA

Coding sequence (CDS)

ATGGGCGACGAGAGGGCTAATATTACTCCAAAACTAACGATTCTTCCTCTCATCGCTTTGATTTTCTACGACGTTTCAGGAGGACCCTTCGGAGTGGAGGATTCCGTGAGCACTGGCGGTGGTCCGCTTCTAGCTTTGCTAGGTTTCTTACTGTTTCCCTTTATTTGGAGTATTCCGGAGGCGTTGGTCACGGCGGAGCTCGCCACAAGCTTCCCCCAAAACGGCGGATATGTGGTCTGGATTTCGGCAGCTTTTGGCCCGTTTTGGGGGTTTCAAGAGGGCTTCTGGAAATGGTTCAGTGGGGTAATGGATAATGCTCTGTATCCTGTACTGTTTCTTGATTACTTGAAGCGTTCGTTTCCTGTTTTTAGCCATATATTTGCTCGAATTCCAGCTCTGTTAGGAATCACTGCTTCTTTAACTTACTTGAACTATCGTGGTCTGCATATTGTTGGGTTTTCTGCTGTTGTTCTTGCTGTGTTTTCGCTTTGTCCTTTTGTAGTGATGACCATTCTTTCAATTCCCAGAATAAGGCCTAGGAAGTGGCTAGTTGTAGATTATAGTATGGTGAATTGGAGGGGCTATTTCAATAGTATGTTTTGGAACCTGAACTATTGGGATAAGGCAAGTACTTTAGCTGGGGAGGTTGAGAATCCTAGCAAGACCTTCCCCAAGGCTATGTTTGGGGCTGTGGTTTTGGTGGTTTGTTCTTATTTGGTTCCTCTTTTGGCTGGGACTGGTGCGTTGGAGACAGATTCAAGTGAGTGGAGTGATGGGTATTTTGCTGAGGTTGGGGCGTTGATTGGTGGGGCGTGGCTGAAGTGGTGGATTCAAGCGGCTGCTGCTATGTCTAACATGGGGTTGTTTGAGGCTGAAATGAGTAGTGATGCATATCAACTGCTTGGGATGAGTGAGATGGGCATGATTCCATCTGTGTTTGCTTCAAGATCAAAATATGGGACACCCACCTTCAGCATTCTGTGCTCTGCCATTGGGGTTATCTTCCTCTCATGGATGAGTTTCCAAGAAATACTCGAGTTTCTCAACTTCCTATACTCCATAGGAATGCTTTTGGAGCTTGCTGCCTTTATAAAACTTAGAATAAAGAAGCCAGACCTCCACAGACCTTACAAAGTTCCCTTGCAAACATTTGGTGCCATAATGCTTTGCTTGCCACCTGCAGCTTTGCTTGTTCTTGTCATGTGTTTAGCTTCTGCACAAACATTCTTAATCAGTGGGGTTATAATTGCTGTAGGGTTTCTTCTATACCCTTCTCTTTCACAAGCGAAGAACAGAGGATGGGTCAAATTTATTTCAGAACAGCAAGGAGATACTCTTCCAGATGTGGAAGACTGCCTGGTCGAGCCGCAACCACGGCAAGAAGTTCTCGATGAGTCAGTGATTCGGCTTCTTTCAGAATCTCCTTCGTCTCCGAATATAGCCAACCAATAA

Protein sequence

MGDERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSSPNIANQ
Homology
BLAST of Carg16340 vs. NCBI nr
Match: XP_022952576.1 (probable polyamine transporter At3g19553 [Cucurbita moschata] >KAG6571928.1 putative polyamine transporter, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011615.1 putative polyamine transporter [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 957.2 bits (2473), Expect = 5.3e-275
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MGDERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPE 60
           MGDERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPE
Sbjct: 1   MGDERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPE 60

Query: 61  ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF 120
           ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF
Sbjct: 61  ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF 120

Query: 121 PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR 180
           PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR
Sbjct: 121 PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR 180

Query: 181 KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV 240
           KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV
Sbjct: 181 KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV 240

Query: 241 PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL 300
           PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL
Sbjct: 241 PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL 300

Query: 301 LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL 360
           LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL
Sbjct: 301 LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL 360

Query: 361 AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF 420
           AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF
Sbjct: 361 AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF 420

Query: 421 LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSSPNI 480
           LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSSPNI
Sbjct: 421 LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSSPNI 480

Query: 481 ANQ 484
           ANQ
Sbjct: 481 ANQ 483

BLAST of Carg16340 vs. NCBI nr
Match: XP_022972448.1 (probable polyamine transporter At3g19553 [Cucurbita maxima])

HSP 1 Score: 948.0 bits (2449), Expect = 3.2e-272
Identity = 476/483 (98.55%), Postives = 481/483 (99.59%), Query Frame = 0

Query: 1   MGDERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPE 60
           MGDERA I PKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWSIPE
Sbjct: 1   MGDERAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPE 60

Query: 61  ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF 120
           ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF
Sbjct: 61  ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF 120

Query: 121 PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR 180
           PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR
Sbjct: 121 PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR 180

Query: 181 KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV 240
           KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV
Sbjct: 181 KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV 240

Query: 241 PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL 300
           PLLAGTGALETDSS+WSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL
Sbjct: 241 PLLAGTGALETDSSDWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL 300

Query: 301 LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL 360
           LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL
Sbjct: 301 LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL 360

Query: 361 AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF 420
           AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISG+II+VGF
Sbjct: 361 AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGIIISVGF 420

Query: 421 LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSSPNI 480
           LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDE+VIRLLSESPSSPNI
Sbjct: 421 LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDEAVIRLLSESPSSPNI 480

Query: 481 ANQ 484
           ANQ
Sbjct: 481 ANQ 483

BLAST of Carg16340 vs. NCBI nr
Match: XP_023553804.1 (probable polyamine transporter At3g19553 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 940.6 bits (2430), Expect = 5.2e-270
Identity = 474/483 (98.14%), Postives = 480/483 (99.38%), Query Frame = 0

Query: 1   MGDERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPE 60
           MGDERANI PKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWSIPE
Sbjct: 1   MGDERANIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPE 60

Query: 61  ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF 120
           ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF
Sbjct: 61  ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF 120

Query: 121 PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR 180
           PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR
Sbjct: 121 PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR 180

Query: 181 KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV 240
           KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV
Sbjct: 181 KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV 240

Query: 241 PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL 300
           PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL
Sbjct: 241 PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL 300

Query: 301 LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL 360
           LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL
Sbjct: 301 LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL 360

Query: 361 AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF 420
           AAFIKLR+KKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF
Sbjct: 361 AAFIKLRMKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF 420

Query: 421 LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSSPNI 480
           LLYPSLSQAKNRGWVKFIS++QG TLPDVED LVEPQPR+EVLDE+VIRLLSESPSSPNI
Sbjct: 421 LLYPSLSQAKNRGWVKFISDEQGATLPDVEDRLVEPQPREEVLDEAVIRLLSESPSSPNI 480

Query: 481 ANQ 484
           ANQ
Sbjct: 481 ANQ 483

BLAST of Carg16340 vs. NCBI nr
Match: XP_038888395.1 (probable polyamine transporter At3g19553 [Benincasa hispida])

HSP 1 Score: 857.8 bits (2215), Expect = 4.4e-245
Identity = 432/486 (88.89%), Postives = 455/486 (93.62%), Query Frame = 0

Query: 1   MGDER---ANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWS 60
           MGDER   A I  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRADAKIAQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120

Query: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180
           RSFPVF+H+FARIP LLGITASLTYLNYRGLHIVG SAVVLA FSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFNHVFARIPTLLGITASLTYLNYRGLHIVGVSAVVLAAFSLCPFVVMTLLSIPRI 180

Query: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240
           RP+KWLVV+YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVV S
Sbjct: 181 RPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALE+DSSEWSDGYFAEVGALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALESDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGM+PSVFA+RSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMLPSVFAARSKYGTPTFSILCSAMGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420
           LE AAFIKLRIKKPDL+RPY+VPLQTFG  MLC PPAALLVLVMCLASA+TFLISG+I+ 
Sbjct: 361 LEFAAFIKLRIKKPDLNRPYRVPLQTFGVTMLCFPPAALLVLVMCLASAKTFLISGIIVT 420

Query: 421 VGFLLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSS 480
           VGFLLYP+L QAKN+ WVKFISEQ   TLPDVED LVE   +QEV +E+ +RLLSES SS
Sbjct: 421 VGFLLYPTLLQAKNKRWVKFISEQPEATLPDVEDRLVESHAQQEVPNEAEVRLLSESSSS 480

Query: 481 PNIANQ 484
            NIA Q
Sbjct: 481 SNIAQQ 486

BLAST of Carg16340 vs. NCBI nr
Match: XP_004136943.1 (probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypothetical protein Csa_017296 [Cucumis sativus])

HSP 1 Score: 853.2 bits (2203), Expect = 1.1e-243
Identity = 436/487 (89.53%), Postives = 456/487 (93.63%), Query Frame = 0

Query: 1   MGDER---ANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWS 60
           MGDER   A IT KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180
           RSFPVF+HIFARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240
            P+KWLVV+YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVV  
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALETDSSEWSDGYFAEVGALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGM+PSVFASRSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLY+IGML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420
           LE AAFIKLRI+KPDL+RPYKVPLQTFG  +LC PPAALL LVMCLASA+TFLISG+IIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPSLSQAKNRGWVKFISEQQGD-TLPDVEDCLVEPQPRQEVLDESVIRLLSESPS 480
           VGFLLYP+L QAKNR WVKFISEQ  D TLPDVED LVE Q +QEV +E+ +RLLSES S
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSES-S 480

Query: 481 SPNIANQ 484
           S NIA Q
Sbjct: 481 SSNIAQQ 486

BLAST of Carg16340 vs. ExPASy Swiss-Prot
Match: Q9LH39 (Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g19553 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 7.9e-205
Identity = 356/464 (76.72%), Postives = 402/464 (86.64%), Query Frame = 0

Query: 9   TPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALVTAELA 68
           +PKLT+LPL+ LIFY+VSGGPFGVEDSV +GGGPLLALLGFL+FP IWSIPEALVTAELA
Sbjct: 19  SPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWSIPEALVTAELA 78

Query: 69  TSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFSHIFA 128
           TSFP+NGGYVVWIS+AFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK SFPV  H+ A
Sbjct: 79  TSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKHSFPVLDHVAA 138

Query: 129 RIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWLVVDYS 188
           R+PALL IT SLTYLNYRGLHIVGFSAVVLAVFSLCPFVVM +L++P IRP++WL VD  
Sbjct: 139 RVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNIRPKRWLFVDTQ 198

Query: 189 MVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLLAGTGA 248
            +NWRGYFN+MFWNLNYWDKASTLAGEV+ P KTFPKA+FGAV+LV+ SYL+PL+AGTGA
Sbjct: 199 KINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGSYLIPLMAGTGA 258

Query: 249 LETDSS-EWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMG 308
           L + +S EWSDGYFAEVG LIGG WLK WIQAAAAMSN+GLFEAEMSSDA+QLLGMSE+G
Sbjct: 259 LSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSDAFQLLGMSEIG 318

Query: 309 MIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFIKLR 368
           M+P+ FA RSKYGTPT SILCSA GVIFLSWMSFQEI+EFLNFLY++GMLLE AAF+KLR
Sbjct: 319 MLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGMLLEFAAFVKLR 378

Query: 369 IKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLYPSLS 428
           IKKPDLHRPY+VPL TFG  MLCLPP+ L++LVM LA+ +TFLISGVII +GF LYP L+
Sbjct: 379 IKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVIIVLGFCLYPFLT 438

Query: 429 QAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLL 472
             K + W +FI E   +T P V     E Q  +E  DES   LL
Sbjct: 439 LVKEKQWARFIPE---ETRP-VSGVSSESQLDEEHGDESAASLL 478

BLAST of Carg16340 vs. ExPASy Swiss-Prot
Match: Q9C6S5 (Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g31830 PE=2 SV=1)

HSP 1 Score: 540.4 bits (1391), Expect = 2.0e-152
Identity = 256/440 (58.18%), Postives = 337/440 (76.59%), Query Frame = 0

Query: 11  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALVTAELATS 70
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF++FPFIWSIPEAL+TAE+ T 
Sbjct: 48  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 107

Query: 71  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFSHIFARI 130
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SGV+DNALYPVLFLDYLK   P       R+
Sbjct: 108 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 167

Query: 131 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWLVVDYSMV 190
            ++L +T  LTYLNYRGL IVG+ AV++ VFS+ PF VM ++SIP++ P +WLV+D   V
Sbjct: 168 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 227

Query: 191 NWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLLAGTGALE 250
           NW  Y N++FWNLNYWD  STLAGEVENP+ T PKA+F  V+LV CSY+ PLLAG GA+ 
Sbjct: 228 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 287

Query: 251 TDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 310
            +  +W+DGYF++V   +GGAWL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 288 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 347

Query: 311 SVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFIKLRIKK 370
             FA RS+YGTP   IL SA GV+ LSW+SFQEI+   N LY +GM+LE  AF+++R+K 
Sbjct: 348 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 407

Query: 371 PDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLYPSLSQAK 430
           P   RPYK+P+ T G+I++C+PP  L+  V+ L+S +   +S V++ +GFL++P L+   
Sbjct: 408 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 467

Query: 431 NRGWVKFISEQQGDTLPDVE 451
            + WVKF        LPD++
Sbjct: 468 RKRWVKF---SISSDLPDLQ 483

BLAST of Carg16340 vs. ExPASy Swiss-Prot
Match: Q9FFL1 (Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 8.6e-151
Identity = 253/433 (58.43%), Postives = 330/433 (76.21%), Query Frame = 0

Query: 7   NITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALVTAE 66
           N   K+T+LPL+ LIFY+VSGGPFG+EDSV    GPLLA++GF++FPFIWSIPEAL+TAE
Sbjct: 48  NQIKKITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAE 107

Query: 67  LATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFSHI 126
           + T FP+NGGYVVW++ A GP+WGFQ+G+ KW SGV+DNALYP+LFLDYLK   P+    
Sbjct: 108 MGTMFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSG 167

Query: 127 FARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWLVVD 186
             R+ A+L +T +LTYLNYRGL IVG +AV+L VFS+ PFVVM+ +SIP+++P +WLVV 
Sbjct: 168 IPRVAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVS 227

Query: 187 YSM--VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLLA 246
             M  VNW  Y N++FWNLNYWD  STL GEVENPSKT P+A+F A++LVV SY+ P+L 
Sbjct: 228 KKMKGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLT 287

Query: 247 GTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMS 306
           GTGA+  D   W+DGYFA++G +IGG WL WWIQAAAA SNMG+F AEMSSD++QLLGM+
Sbjct: 288 GTGAIALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMA 347

Query: 307 EMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFI 366
           E GM+P VFA RS+Y TP   IL SA GVI LSW+SFQEI+   N LY  GM+LE   F+
Sbjct: 348 ERGMLPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFV 407

Query: 367 KLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLYP 426
           +LR+K P   RP+K+P+   G++++C+PP  L+ ++M   + +  L+S   I +G +L P
Sbjct: 408 RLRMKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQP 467

Query: 427 SLSQAKNRGWVKF 438
            L Q + +GW+KF
Sbjct: 468 CLKQVEKKGWLKF 479

BLAST of Carg16340 vs. ExPASy Swiss-Prot
Match: A2X8M8 (Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 3.7e-146
Identity = 249/426 (58.45%), Postives = 328/426 (77.00%), Query Frame = 0

Query: 12  LTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALVTAELATSF 71
           ++++PLI LIFY+VSGGPFG+EDSV    GPLLA++GFL+ P IWSIPEAL+TAEL   F
Sbjct: 83  VSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSIPEALITAELGAMF 142

Query: 72  PQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFSHIFARIP 131
           P+NGGYVVW+++A GP+WGFQ+G+ KW SGV+DNALYPVLFLDYLK   P       R  
Sbjct: 143 PENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAPRAF 202

Query: 132 ALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWLVVDYSMVN 191
           A++G+TA LT LNYRGL +VG+ A+ L VFSL PF VM ++++P++RP +WLV+D   V+
Sbjct: 203 AVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRPARWLVIDLHNVD 262

Query: 192 WRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLLAGTGALET 251
           W  Y N++FWNLNYWD  STLAGEV+NP KT PKA+F AV+ VV +YL PLLAGTGA+  
Sbjct: 263 WNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGAVPL 322

Query: 252 DSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIPS 311
           D  +W+DGYFA++  L+GGAWL WW+Q+AAA+SNMG+F AEMSSD+YQLLGM+E GM+PS
Sbjct: 323 DRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGMLPS 382

Query: 312 VFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFIKLRIKKP 371
            FA+RS+YGTP   IL SA GV+ LS MSFQEI+   NFLY  GMLLE  AFI  R+++P
Sbjct: 383 FFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRVRRP 442

Query: 372 DLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLYPSLSQAKN 431
           D  RPY+VPL T G + + +PP AL+ +V+ L++ +  ++S   +A+G +L P+L   + 
Sbjct: 443 DAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRFVEK 502

Query: 432 RGWVKF 438
           + W++F
Sbjct: 503 KRWLRF 507

BLAST of Carg16340 vs. ExPASy Swiss-Prot
Match: Q6Z8D0 (Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 3.7e-146
Identity = 249/426 (58.45%), Postives = 328/426 (77.00%), Query Frame = 0

Query: 12  LTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALVTAELATSF 71
           ++++PLI LIFY+VSGGPFG+EDSV    GPLLA++GFL+ P IWSIPEAL+TAEL   F
Sbjct: 83  VSMIPLIFLIFYEVSGGPFGIEDSVG-AAGPLLAIIGFLVLPVIWSIPEALITAELGAMF 142

Query: 72  PQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFSHIFARIP 131
           P+NGGYVVW+++A GP+WGFQ+G+ KW SGV+DNALYPVLFLDYLK   P       R  
Sbjct: 143 PENGGYVVWVASALGPYWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGGGAPRAF 202

Query: 132 ALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWLVVDYSMVN 191
           A++G+TA LT LNYRGL +VG+ A+ L VFSL PF VM ++++P++RP +WLV+D   V+
Sbjct: 203 AVVGLTAVLTLLNYRGLTVVGWVAICLGVFSLLPFFVMGLIALPKLRPARWLVIDLHNVD 262

Query: 192 WRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLLAGTGALET 251
           W  Y N++FWNLNYWD  STLAGEV+NP KT PKA+F AV+ VV +YL PLLAGTGA+  
Sbjct: 263 WNLYLNTLFWNLNYWDSISTLAGEVKNPGKTLPKALFYAVIFVVVAYLYPLLAGTGAVPL 322

Query: 252 DSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIPS 311
           D  +W+DGYFA++  L+GGAWL WW+Q+AAA+SNMG+F AEMSSD+YQLLGM+E GM+PS
Sbjct: 323 DRGQWTDGYFADIAKLLGGAWLMWWVQSAAALSNMGMFVAEMSSDSYQLLGMAERGMLPS 382

Query: 312 VFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFIKLRIKKP 371
            FA+RS+YGTP   IL SA GV+ LS MSFQEI+   NFLY  GMLLE  AFI  R+++P
Sbjct: 383 FFAARSRYGTPLAGILFSASGVLLLSMMSFQEIVAAENFLYCFGMLLEFVAFILHRVRRP 442

Query: 372 DLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLYPSLSQAKN 431
           D  RPY+VPL T G + + +PP AL+ +V+ L++ +  ++S   +A+G +L P+L   + 
Sbjct: 443 DAARPYRVPLGTAGCVAMLVPPTALIAVVLALSTLKVAVVSLGAVAMGLVLQPALRFVEK 502

Query: 432 RGWVKF 438
           + W++F
Sbjct: 503 KRWLRF 507

BLAST of Carg16340 vs. ExPASy TrEMBL
Match: A0A6J1GKM4 (probable polyamine transporter At3g19553 OS=Cucurbita moschata OX=3662 GN=LOC111455227 PE=3 SV=1)

HSP 1 Score: 957.2 bits (2473), Expect = 2.6e-275
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MGDERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPE 60
           MGDERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPE
Sbjct: 1   MGDERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPE 60

Query: 61  ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF 120
           ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF
Sbjct: 61  ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF 120

Query: 121 PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR 180
           PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR
Sbjct: 121 PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR 180

Query: 181 KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV 240
           KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV
Sbjct: 181 KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV 240

Query: 241 PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL 300
           PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL
Sbjct: 241 PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL 300

Query: 301 LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL 360
           LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL
Sbjct: 301 LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL 360

Query: 361 AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF 420
           AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF
Sbjct: 361 AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF 420

Query: 421 LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSSPNI 480
           LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSSPNI
Sbjct: 421 LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSSPNI 480

Query: 481 ANQ 484
           ANQ
Sbjct: 481 ANQ 483

BLAST of Carg16340 vs. ExPASy TrEMBL
Match: A0A6J1I9Z5 (probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC111471008 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.6e-272
Identity = 476/483 (98.55%), Postives = 481/483 (99.59%), Query Frame = 0

Query: 1   MGDERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPE 60
           MGDERA I PKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWSIPE
Sbjct: 1   MGDERAKIAPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWSIPE 60

Query: 61  ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF 120
           ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF
Sbjct: 61  ALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSF 120

Query: 121 PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR 180
           PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR
Sbjct: 121 PVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPR 180

Query: 181 KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV 240
           KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV
Sbjct: 181 KWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLV 240

Query: 241 PLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL 300
           PLLAGTGALETDSS+WSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL
Sbjct: 241 PLLAGTGALETDSSDWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQL 300

Query: 301 LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL 360
           LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL
Sbjct: 301 LGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLEL 360

Query: 361 AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGF 420
           AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISG+II+VGF
Sbjct: 361 AAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGIIISVGF 420

Query: 421 LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLLSESPSSPNI 480
           LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDE+VIRLLSESPSSPNI
Sbjct: 421 LLYPSLSQAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDEAVIRLLSESPSSPNI 480

Query: 481 ANQ 484
           ANQ
Sbjct: 481 ANQ 483

BLAST of Carg16340 vs. ExPASy TrEMBL
Match: A0A0A0K2Y2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.3e-244
Identity = 436/487 (89.53%), Postives = 456/487 (93.63%), Query Frame = 0

Query: 1   MGDER---ANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWS 60
           MGDER   A IT KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRVDAKITQKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180
           RSFPVF+HIFARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240
            P+KWLVV+YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVV  
Sbjct: 181 SPKKWLVVEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSF 240

Query: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGALETDSSEWSDGYFAEVGALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALETDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGM+PSVFASRSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLY+IGML
Sbjct: 301 YQLLGMSEMGMLPSVFASRSKYGTPTFSILCSALGVIFLSWMSFQEILEFLNFLYAIGML 360

Query: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420
           LE AAFIKLRI+KPDL+RPYKVPLQTFG  +LC PPAALL LVMCLASA+TFLISG+IIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTFGVTLLCFPPAALLFLVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPSLSQAKNRGWVKFISEQQGD-TLPDVEDCLVEPQPRQEVLDESVIRLLSESPS 480
           VGFLLYP+L QAKNR WVKFISEQ  D TLPDVED LVE Q +QEV +E+ +RLLSES S
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLPDVEDRLVESQQQQEVPNEAEVRLLSES-S 480

Query: 481 SPNIANQ 484
           S NIA Q
Sbjct: 481 SSNIAQQ 486

BLAST of Carg16340 vs. ExPASy TrEMBL
Match: A0A5D3C4H2 (Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001140 PE=3 SV=1)

HSP 1 Score: 852.4 bits (2201), Expect = 9.0e-244
Identity = 433/487 (88.91%), Postives = 456/487 (93.63%), Query Frame = 0

Query: 1   MGDER---ANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWS 60
           MGDER   A I  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180
           RSFPVF+HIFARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240
            P+KWL+++YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVV S
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVSS 240

Query: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGAL TDSSEWSDGYFAEVGALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420
           LE AAFIKLRI+KPDL+RPYKVPLQT G  +LC PP+ALL+LVMCLASA+TFLISG+IIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPSLSQAKNRGWVKFISEQQGD-TLPDVEDCLVEPQPRQEVLDESVIRLLSESPS 480
           VGFLLYP+L QAKNR WVKFISEQ  D TL DVED LVEPQP+QEV +E+ +RLLSES S
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES-S 480

Query: 481 SPNIANQ 484
           S NIA Q
Sbjct: 481 SSNIAQQ 486

BLAST of Carg16340 vs. ExPASy TrEMBL
Match: A0A1S3C014 (probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495296 PE=3 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 2.0e-243
Identity = 432/487 (88.71%), Postives = 456/487 (93.63%), Query Frame = 0

Query: 1   MGDER---ANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWS 60
           MGDER   A I  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFL+FPFIWS
Sbjct: 1   MGDERRVDAKIAKKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLVFPFIWS 60

Query: 61  IPEALVTAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK 120
           IPEALVTAELAT FPQNGGYV+WISAAFGPFWGFQEGFWKWFSG MDNALYPVLFLDYLK
Sbjct: 61  IPEALVTAELATIFPQNGGYVIWISAAFGPFWGFQEGFWKWFSGAMDNALYPVLFLDYLK 120

Query: 121 RSFPVFSHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRI 180
           RSFPVF+HIFARIPALLGITASLTYLNYRGLHIVG SAVVLAVFSLCPFVVMT+LSIPRI
Sbjct: 121 RSFPVFNHIFARIPALLGITASLTYLNYRGLHIVGVSAVVLAVFSLCPFVVMTLLSIPRI 180

Query: 181 RPRKWLVVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCS 240
            P+KWL+++YS VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFP+AMFGAVVLVV S
Sbjct: 181 SPKKWLILEYSKVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPRAMFGAVVLVVSS 240

Query: 241 YLVPLLAGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDA 300
           YL+PLLAGTGAL TDSSEWSDGYFAEVGALIGG WLKWWIQAAAAMSNMGLFEAEMSSDA
Sbjct: 241 YLIPLLAGTGALATDSSEWSDGYFAEVGALIGGVWLKWWIQAAAAMSNMGLFEAEMSSDA 300

Query: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGML 360
           YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSA+GVIFLSWMSFQEILEFLNFLYS+GML
Sbjct: 301 YQLLGMSEMGMIPSVFASRSKYGTPTFSILCSAVGVIFLSWMSFQEILEFLNFLYSVGML 360

Query: 361 LELAAFIKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIA 420
           LE AAFIKLRI+KPDL+RPYKVPLQT G  +LC PP+ALL+LVMCLASA+TFLISG+IIA
Sbjct: 361 LEFAAFIKLRIRKPDLNRPYKVPLQTVGVTLLCFPPSALLILVMCLASAKTFLISGIIIA 420

Query: 421 VGFLLYPSLSQAKNRGWVKFISEQQGD-TLPDVEDCLVEPQPRQEVLDESVIRLLSESPS 480
           VGFLLYP+L QAKNR WVKFISEQ  D TL DVED LVEPQP+QEV +E+ +RLLSES S
Sbjct: 421 VGFLLYPTLLQAKNRRWVKFISEQPEDTTLSDVEDRLVEPQPQQEVPNEAEVRLLSES-S 480

Query: 481 SPNIANQ 484
           S NIA Q
Sbjct: 481 SSNIAQQ 486

BLAST of Carg16340 vs. TAIR 10
Match: AT3G19553.1 (Amino acid permease family protein )

HSP 1 Score: 714.5 bits (1843), Expect = 5.6e-206
Identity = 356/464 (76.72%), Postives = 402/464 (86.64%), Query Frame = 0

Query: 9   TPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALVTAELA 68
           +PKLT+LPL+ LIFY+VSGGPFGVEDSV +GGGPLLALLGFL+FP IWSIPEALVTAELA
Sbjct: 19  SPKLTLLPLVFLIFYEVSGGPFGVEDSVKSGGGPLLALLGFLIFPLIWSIPEALVTAELA 78

Query: 69  TSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFSHIFA 128
           TSFP+NGGYVVWIS+AFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLK SFPV  H+ A
Sbjct: 79  TSFPENGGYVVWISSAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKHSFPVLDHVAA 138

Query: 129 RIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWLVVDYS 188
           R+PALL IT SLTYLNYRGLHIVGFSAVVLAVFSLCPFVVM +L++P IRP++WL VD  
Sbjct: 139 RVPALLVITFSLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMALLAVPNIRPKRWLFVDTQ 198

Query: 189 MVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLLAGTGA 248
            +NWRGYFN+MFWNLNYWDKASTLAGEV+ P KTFPKA+FGAV+LV+ SYL+PL+AGTGA
Sbjct: 199 KINWRGYFNTMFWNLNYWDKASTLAGEVDRPGKTFPKALFGAVLLVMGSYLIPLMAGTGA 258

Query: 249 LETDSS-EWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMG 308
           L + +S EWSDGYFAEVG LIGG WLK WIQAAAAMSN+GLFEAEMSSDA+QLLGMSE+G
Sbjct: 259 LSSSTSGEWSDGYFAEVGMLIGGVWLKGWIQAAAAMSNLGLFEAEMSSDAFQLLGMSEIG 318

Query: 309 MIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFIKLR 368
           M+P+ FA RSKYGTPT SILCSA GVIFLSWMSFQEI+EFLNFLY++GMLLE AAF+KLR
Sbjct: 319 MLPAFFAQRSKYGTPTISILCSATGVIFLSWMSFQEIIEFLNFLYALGMLLEFAAFVKLR 378

Query: 369 IKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLYPSLS 428
           IKKPDLHRPY+VPL TFG  MLCLPP+ L++LVM LA+ +TFLISGVII +GF LYP L+
Sbjct: 379 IKKPDLHRPYRVPLNTFGVSMLCLPPSLLVILVMVLAAPKTFLISGVIIVLGFCLYPFLT 438

Query: 429 QAKNRGWVKFISEQQGDTLPDVEDCLVEPQPRQEVLDESVIRLL 472
             K + W +FI E   +T P V     E Q  +E  DES   LL
Sbjct: 439 LVKEKQWARFIPE---ETRP-VSGVSSESQLDEEHGDESAASLL 478

BLAST of Carg16340 vs. TAIR 10
Match: AT1G31830.1 (Amino acid permease family protein )

HSP 1 Score: 540.4 bits (1391), Expect = 1.5e-153
Identity = 256/440 (58.18%), Postives = 337/440 (76.59%), Query Frame = 0

Query: 11  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALVTAELATS 70
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF++FPFIWSIPEAL+TAE+ T 
Sbjct: 48  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 107

Query: 71  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFSHIFARI 130
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SGV+DNALYPVLFLDYLK   P       R+
Sbjct: 108 YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 167

Query: 131 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWLVVDYSMV 190
            ++L +T  LTYLNYRGL IVG+ AV++ VFS+ PF VM ++SIP++ P +WLV+D   V
Sbjct: 168 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 227

Query: 191 NWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLLAGTGALE 250
           NW  Y N++FWNLNYWD  STLAGEVENP+ T PKA+F  V+LV CSY+ PLLAG GA+ 
Sbjct: 228 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 287

Query: 251 TDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 310
            +  +W+DGYF++V   +GGAWL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 288 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 347

Query: 311 SVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFIKLRIKK 370
             FA RS+YGTP   IL SA GV+ LSW+SFQEI+   N LY +GM+LE  AF+++R+K 
Sbjct: 348 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 407

Query: 371 PDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLYPSLSQAK 430
           P   RPYK+P+ T G+I++C+PP  L+  V+ L+S +   +S V++ +GFL++P L+   
Sbjct: 408 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 467

Query: 431 NRGWVKFISEQQGDTLPDVE 451
            + WVKF        LPD++
Sbjct: 468 RKRWVKF---SISSDLPDLQ 483

BLAST of Carg16340 vs. TAIR 10
Match: AT1G31830.2 (Amino acid permease family protein )

HSP 1 Score: 540.4 bits (1391), Expect = 1.5e-153
Identity = 256/440 (58.18%), Postives = 337/440 (76.59%), Query Frame = 0

Query: 11  KLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALVTAELATS 70
           K+++LPL+ LIFY+VSGGPFGVEDSV+   GPLLALLGF++FPFIWSIPEAL+TAE+ T 
Sbjct: 32  KVSMLPLVFLIFYEVSGGPFGVEDSVN-AAGPLLALLGFVIFPFIWSIPEALITAEMGTM 91

Query: 71  FPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFSHIFARI 130
           +P+NGGYVVW+S+A GPFWGFQ+G+ KW SGV+DNALYPVLFLDYLK   P       R+
Sbjct: 92  YPENGGYVVWVSSALGPFWGFQQGWMKWLSGVIDNALYPVLFLDYLKSGVPALGSGLPRV 151

Query: 131 PALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWLVVDYSMV 190
            ++L +T  LTYLNYRGL IVG+ AV++ VFS+ PF VM ++SIP++ P +WLV+D   V
Sbjct: 152 ASILVLTILLTYLNYRGLTIVGWVAVLMGVFSILPFAVMGLISIPQLEPSRWLVMDLGNV 211

Query: 191 NWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLLAGTGALE 250
           NW  Y N++FWNLNYWD  STLAGEVENP+ T PKA+F  V+LV CSY+ PLLAG GA+ 
Sbjct: 212 NWNLYLNTLFWNLNYWDSISTLAGEVENPNHTLPKALFYGVILVACSYIFPLLAGIGAIP 271

Query: 251 TDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMSEMGMIP 310
            +  +W+DGYF++V   +GGAWL+WW+QAAAA SNMG+F AEMSSD++QLLGM+E GM+P
Sbjct: 272 LEREKWTDGYFSDVAKALGGAWLRWWVQAAAATSNMGMFIAEMSSDSFQLLGMAERGMLP 331

Query: 311 SVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFIKLRIKK 370
             FA RS+YGTP   IL SA GV+ LSW+SFQEI+   N LY +GM+LE  AF+++R+K 
Sbjct: 332 EFFAKRSRYGTPLLGILFSASGVVLLSWLSFQEIVAAENLLYCVGMILEFIAFVRMRMKH 391

Query: 371 PDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLYPSLSQAK 430
           P   RPYK+P+ T G+I++C+PP  L+  V+ L+S +   +S V++ +GFL++P L+   
Sbjct: 392 PAASRPYKIPIGTTGSILMCIPPTILICAVVALSSLKVAAVSIVMMIIGFLIHPLLNHMD 451

Query: 431 NRGWVKFISEQQGDTLPDVE 451
            + WVKF        LPD++
Sbjct: 452 RKRWVKF---SISSDLPDLQ 467

BLAST of Carg16340 vs. TAIR 10
Match: AT5G05630.1 (Amino acid permease family protein )

HSP 1 Score: 535.0 bits (1377), Expect = 6.1e-152
Identity = 253/433 (58.43%), Postives = 330/433 (76.21%), Query Frame = 0

Query: 7   NITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALVTAE 66
           N   K+T+LPL+ LIFY+VSGGPFG+EDSV    GPLLA++GF++FPFIWSIPEAL+TAE
Sbjct: 48  NQIKKITVLPLVFLIFYEVSGGPFGIEDSVK-AAGPLLAIVGFIVFPFIWSIPEALITAE 107

Query: 67  LATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVFSHI 126
           + T FP+NGGYVVW++ A GP+WGFQ+G+ KW SGV+DNALYP+LFLDYLK   P+    
Sbjct: 108 MGTMFPENGGYVVWVTLAMGPYWGFQQGWVKWLSGVIDNALYPILFLDYLKSGIPILGSG 167

Query: 127 FARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWLVVD 186
             R+ A+L +T +LTYLNYRGL IVG +AV+L VFS+ PFVVM+ +SIP+++P +WLVV 
Sbjct: 168 IPRVAAILVLTVALTYLNYRGLSIVGVAAVLLGVFSILPFVVMSFMSIPKLKPSRWLVVS 227

Query: 187 YSM--VNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLLA 246
             M  VNW  Y N++FWNLNYWD  STL GEVENPSKT P+A+F A++LVV SY+ P+L 
Sbjct: 228 KKMKGVNWSLYLNTLFWNLNYWDSVSTLTGEVENPSKTLPRALFYALLLVVFSYIFPVLT 287

Query: 247 GTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGMS 306
           GTGA+  D   W+DGYFA++G +IGG WL WWIQAAAA SNMG+F AEMSSD++QLLGM+
Sbjct: 288 GTGAIALDQKLWTDGYFADIGKVIGGVWLGWWIQAAAATSNMGMFLAEMSSDSFQLLGMA 347

Query: 307 EMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAFI 366
           E GM+P VFA RS+Y TP   IL SA GVI LSW+SFQEI+   N LY  GM+LE   F+
Sbjct: 348 ERGMLPEVFAKRSRYRTPWVGILFSASGVIILSWLSFQEIVAAENLLYCFGMVLEFITFV 407

Query: 367 KLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLYP 426
           +LR+K P   RP+K+P+   G++++C+PP  L+ ++M   + +  L+S   I +G +L P
Sbjct: 408 RLRMKYPAASRPFKIPVGVLGSVLMCIPPTVLIGVIMAFTNLKVALVSLAAIVIGLVLQP 467

Query: 427 SLSQAKNRGWVKF 438
            L Q + +GW+KF
Sbjct: 468 CLKQVEKKGWLKF 479

BLAST of Carg16340 vs. TAIR 10
Match: AT1G31820.1 (Amino acid permease family protein )

HSP 1 Score: 505.0 bits (1299), Expect = 6.8e-143
Identity = 252/456 (55.26%), Postives = 333/456 (73.03%), Query Frame = 0

Query: 4   ERANITPKLTILPLIALIFYDVSGGPFGVEDSVSTGGGPLLALLGFLLFPFIWSIPEALV 63
           E  N   K+++LPL+ LIFY+VSGGPFG E SV+   GPLLALLGF++FPFIW IPEAL+
Sbjct: 28  EGNNSIQKVSMLPLVFLIFYEVSGGPFGAEGSVN-AAGPLLALLGFVIFPFIWCIPEALI 87

Query: 64  TAELATSFPQNGGYVVWISAAFGPFWGFQEGFWKWFSGVMDNALYPVLFLDYLKRSFPVF 123
           TAE++T FP NGG+VVW+S+A G FWGFQ G+ KW  GV+DNALYPVLFLDYLK + P  
Sbjct: 88  TAEMSTMFPINGGFVVWVSSALGTFWGFQVGWMKWLCGVIDNALYPVLFLDYLKSAVPAL 147

Query: 124 SHIFARIPALLGITASLTYLNYRGLHIVGFSAVVLAVFSLCPFVVMTILSIPRIRPRKWL 183
           +    R+ ++L +T  LTYLNYRGL IVG++AV + VFS+ PF VM+++SIP++ P +WL
Sbjct: 148 ATGLPRVASILILTLLLTYLNYRGLTIVGWTAVFMGVFSMLPFAVMSLVSIPQLEPSRWL 207

Query: 184 VVDYSMVNWRGYFNSMFWNLNYWDKASTLAGEVENPSKTFPKAMFGAVVLVVCSYLVPLL 243
           V+D   VNW  Y N++ WNLNYWD  STLAGEV NP KT PKA+   V+ V  S  +PLL
Sbjct: 208 VMDLGNVNWNLYLNTLLWNLNYWDSVSTLAGEVANPKKTLPKALCYGVIFVALSNFLPLL 267

Query: 244 AGTGALETDSSEWSDGYFAEVGALIGGAWLKWWIQAAAAMSNMGLFEAEMSSDAYQLLGM 303
           +GTGA+  D   W+DGY AEV   IGG WL+ W+QAAAA SNMG+F AEMSSD++QLLGM
Sbjct: 268 SGTGAIPLDRELWTDGYLAEVAKAIGGGWLQLWVQAAAATSNMGMFLAEMSSDSFQLLGM 327

Query: 304 SEMGMIPSVFASRSKYGTPTFSILCSAIGVIFLSWMSFQEILEFLNFLYSIGMLLELAAF 363
           +E+G++P +FA RS+YGTP   IL SA GV+ LS +SFQEI+   N LY  GM+LE  AF
Sbjct: 328 AELGILPEIFAQRSRYGTPLLGILFSASGVLLLSGLSFQEIIAAENLLYCGGMILEFIAF 387

Query: 364 IKLRIKKPDLHRPYKVPLQTFGAIMLCLPPAALLVLVMCLASAQTFLISGVIIAVGFLLY 423
           ++LR K P   RPYK+P+ T G+I++C+PP  L+ LV+ L++ +  L+S V++ +GFL+ 
Sbjct: 388 VRLRKKHPAASRPYKIPVGTVGSILICVPPIVLICLVIVLSTIKVALVSFVMVVIGFLMK 447

Query: 424 PSLSQAKNRGWVKF-----ISEQQGDTLPDVEDCLV 455
           P L+    + WVKF     ++E Q + L D E+ L+
Sbjct: 448 PCLNHMDGKKWVKFSVCSDLAEFQKENL-DCEESLL 481

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022952576.15.3e-275100.00probable polyamine transporter At3g19553 [Cucurbita moschata] >KAG6571928.1 puta... [more]
XP_022972448.13.2e-27298.55probable polyamine transporter At3g19553 [Cucurbita maxima][more]
XP_023553804.15.2e-27098.14probable polyamine transporter At3g19553 [Cucurbita pepo subsp. pepo][more]
XP_038888395.14.4e-24588.89probable polyamine transporter At3g19553 [Benincasa hispida][more]
XP_004136943.11.1e-24389.53probable polyamine transporter At3g19553 [Cucumis sativus] >KGN43858.1 hypotheti... [more]
Match NameE-valueIdentityDescription
Q9LH397.9e-20576.72Probable polyamine transporter At3g19553 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q9C6S52.0e-15258.18Probable polyamine transporter At1g31830 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
Q9FFL18.6e-15158.43Polyamine transporter RMV1 OS=Arabidopsis thaliana OX=3702 GN=RMV1 PE=1 SV=1[more]
A2X8M83.7e-14658.45Polyamine transporter PUT1 OS=Oryza sativa subsp. indica OX=39946 GN=PUT1 PE=3 S... [more]
Q6Z8D03.7e-14658.45Polyamine transporter PUT1 OS=Oryza sativa subsp. japonica OX=39947 GN=PUT1 PE=1... [more]
Match NameE-valueIdentityDescription
A0A6J1GKM42.6e-275100.00probable polyamine transporter At3g19553 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1I9Z51.6e-27298.55probable polyamine transporter At3g19553 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
A0A0A0K2Y25.3e-24489.53Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G071470 PE=3 SV=1[more]
A0A5D3C4H29.0e-24488.91Putative polyamine transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3C0142.0e-24388.71probable polyamine transporter At3g19553 isoform X1 OS=Cucumis melo OX=3656 GN=L... [more]
Match NameE-valueIdentityDescription
AT3G19553.15.6e-20676.72Amino acid permease family protein [more]
AT1G31830.11.5e-15358.18Amino acid permease family protein [more]
AT1G31830.21.5e-15358.18Amino acid permease family protein [more]
AT5G05630.16.1e-15258.43Amino acid permease family protein [more]
AT1G31820.16.8e-14355.26Amino acid permease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePIRSFPIRSF006060AA_transportercoord: 3..438
e-value: 1.5E-66
score: 222.4
NoneNo IPR availableGENE3D1.20.1740.10Amino acid/polyamine transporter Icoord: 11..439
e-value: 8.9E-57
score: 194.6
NoneNo IPR availablePANTHERPTHR45826:SF21BNAA05G20530D PROTEINcoord: 7..470
IPR002293Amino acid/polyamine transporter IPFAMPF13520AA_permease_2coord: 17..417
e-value: 2.1E-34
score: 119.2
IPR044566Polyamine transporter RMV1-likePANTHERPTHR45826POLYAMINE TRANSPORTER PUT1coord: 7..470

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg16340-RACarg16340-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902047 polyamine transmembrane transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015203 polyamine transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity