Carg15322 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg15322
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description28 kDa heat- and acid-stable phosphoprotein-like
LocationCarg_Chr03: 9282822 .. 9286170 (+)
RNA-Seq ExpressionCarg15322
SyntenyCarg15322
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCCATGTCCAGATTTTTTTCCCTCCCAAATTCTTTTTTCGAGGGTTATTTCTCCATATATACCCTCCCCAAAATACCTACCGTTTATATTTAATATGATTATATATTTATTATTATTACTTTCGCTCTGGTTTCCACCCTCTTATAAAAAAAAATATTTTAATCAACGTTGGATTTTACAATCCACTCATTTGGGGCTAGCATCCTCAAGTTTCCACGTTCTTGTAAGTAATATTTCAACCGCTATGTAATCTTACAAACCACTCCTTTGGTGCCCTAGCGTTCATTCTCGAGTTTTTACACCTTTACAAGAAATACTTGAAGCGATGTGAGATCTTACAATCCACCCCTTTGGGATCCAACGTCCTCACTAACACGTCGTTCAGTGTCTAGTTCTGATACAATTTGTAACAGCGCGAACTCACCGCTAGTAAATATTGTCGGCTTTAATTAATTACGTATTGCCATCAAACTGGCGGGTTTGTAATGGAAAATCTAGGATTAATGTGGAGGGTGAAGATATGAAGGAAAGGATAAGCAGGTGATTATTGGGTGCCGGTTCGGGTAGGGTTTGGGAATCCGGTTGGCGATTTGCACCGCAGAGAGATGTAAACGGGACGGGAACAAAGAAGCCAACACAACTATAAATTCACTTCTCGGCGGAAAGGCGGAGAAGTCACTAACTTCGTCTTCCACAACTAAAACTAAGAGGGGACTTCTATTCTACGTCGATTCTGCTTCTCTTCTGCTTCTTCGTCCGATTCTACCTTCTCCCTGCTGCTTTTCGTTGACGATGGGAAGAGGAAAGTACAAGAGCAAGCCCACCGGTCGGCGCCAATTCTCTACCCCTGAAGACATGCGTAAGCAATATTTATCTCTCGTTTCTTTTTAACCACCTTCGGTTGATTATCGAGAAAGTCAAGATCACGAGATCTTTTTCTGCGAGTGCTATCGTCTTCTTGCCTAATGTGTTGATTTTATATATTTGAGGTGATGAATTCGAGTTGTGTTTTTAGGATACGAGATGGTAGTGGACATGGAGAGTCCAGTAGTTGGAATGATGCTGGATATATTAGTGATTTTAGCTGTTTTTATATGTTACTTCTTTATATTTTAGATCGGTTTCGCCGACTATGAGGAGGTGGTTGGTGGTGAGATCTAGGCTTAGTTGATTGTGGAAATTGTTTTACTTTTTGATGTGGATGCTGAAATGATTGTAACCTAAATGAAGCGTCGTGGTTATTGTGCGAGGTGAACGCGTTTTGCTTTGGAATTGCTTCTTAAAACTAGTAGAAGTTGGTAGAGATGGATGGTGTCTATGAATTAATCTTTTAAAGCAAAGCCCAAAACTTGATCACAACGCATTATGTAAATACGCTTGTGATTAGAGTTCTTCCGTTCTTCAGATTCACCAATAGAAGAACTGCCATACTAACTAAAGATTTCATTGTTGAGAAAGTTTAAGATGGCTGAAAATTGCACTGCTAACTTCAGTTTCTGAATTAATTGTAGTTGCCGGAACCTCCACGCGTCCACGCACGTTCAAACAGGTTCTCTCTCTCTCTCTCTCTCTGTATCTTAAGACACGCACACACTTAGTTCCCTCTACCAACAACATATAATTACAATGCCCGGTAGTTGGGTTTGTCAATTGGGTATGGATAATACTCTGATGTGTTGCAACTTTTCCTTCTGCTGCTGCTTCTTCAACTGCATGGTCTTGTTCTTTCTATTTACTTATTTTTCTGCACATGATGTATTTAATTTATTATTATAGGTAGAAGCTGAACACAAGGAGGAGGAAGAATCTGAGGAAGGATCCGAACAAGAATCGGGAGATGAAACTGATGATGAGGATAAACCTAGAGTTTGTATTCAATCACATTTTTTTTTCTTTAATCTACAAGTCTGATCGTCTACATATTTTGTTGTTTGTTTGAGCTGAGCGAGACTAAGTCACTTAATATGTTGTTAAATTCTGCCAGAGGAAAGGCACTCAAGGTATTATTGAGATTGAAAATCCTAACTTGGTGAAGCCAAAAACCTTGAAAGCTAGAGATATTGATGTAAGTTTAATAATCATCCTTACGGAAGGTTTTGCATTGAACATTGTTATACAGTGGAGGTGTAATTTCACTTCATTTTGCAGATTGGGAAAACAACTGAACTTTCAAGGCGTGAAAGGTTGTTACAAAGCTCTATTTTCTTTGTTTTAACGAATATTTAACATTTACTTAATTTTTATGTTTATAGTTGATTTTTCTTTAATTCTTTTTCCAGAGAGGAGATAGAGAAACAAAAGGCTCATGAGCGATACATGAGGCTTCAAGAACAAGGGAAAACTGAACAAGCTAGGAAAGATTTAGGTAATCCTCTTTCAATGATCAATTCTCTGGTTTAAGTGTGTGTTCTATGTTCTGAGGATGAAACCTTTTTATTATCGATGTGTCATCCAAGATTCATATGCATTTTCTTTCATTCTTTTCCTGAGTTAAAAGGGAAAAACATATAGGAATGCTCAGAATGATCACCTGCTGTTGCACTTGCTTTGTATTCAGCGTCTTGCACATTCACATTGTATAAATTTGGTTCAAAATGCGCTTTGATAACATCATGGGGAACCAATGCTATGTCCTGAACAACTTTTTTCATGTGCACCATTGATAGCTGTTGAAGGGTGTGAAAAAAATTTGAAGCCATAACTTTCATTGCACTGTCTTAGAACTCAACGATACAGTTATACAGCCTAAAGAACAGATTCTGTTCAAGTAAGGTCATAATTGGTTTGTCTGATATTGAAATTTTATTTCACCATATGCTGTGCAGAACGTTTGTCTCTTATTCGTCAACAAAGGGCAGAAGCTGCTAAAAAAAGAGAAGAAGAAAAGGCTGGTAATATATACAACAGAACACTATCACTATATAGTTCTTATAGTAACATTTCCTTGTAACATTGAAACTGATATCCACTCTCTCCCCCTCACTTTCAGCCAAAGAACAGAAGAAAGCAGAAGCTCGCAAATGAGCTCCGGGACCCTCCGAGTTCTTTTCTCTGGGACGAAAGACATGTAATCTCTACAATTTTACTTTCTAGTTTGCCTAGACTAATATTGTCAGCAACCAAGTTATATTCTCTTATTTGGCACAGAAGTGACCATAGTATTAAGCCGTTTTACATTTATAATTTACGCAAGTATACTTCTTATATCTCTACGGCATAAGAGGTTTGATTCAATATGACTTTAATATTAGGTTATAACCTTTATTCTCTACGCCTTTATACCTTATCAATTTAGAATATGTTTCCATTTTCTTCTTTAAATATTGGCCTAGAATACTTCTGAGA

mRNA sequence

ATGTTCCATGGTTTGGGAATCCGGTTGGCGATTTGCACCGCAGAGAGATGTAAACGGGACGGGAACAAAGAAGCCAACACAACTATAAATTCACTTCTCGGCGGAAAGGCGGAGAAGTCACTAACTTCGTCTTCCACAACTAAAACTAAGAGGGGACTTCTATTCTACGTCGATTCTGCTTCTCTTCTGCTTCTTCGTCCGATTCTACCTTCTCCCTGCTGCTTTTCGTTGACGATGGGAAGAGGAAAGTACAAGAGCAAGCCCACCGGTCGGCGCCAATTCTCTACCCCTGAAGACATGCGATACGAGATGGTAGTGGACATGGAGAGTCCAGTAGTTGGAATGATGCTGGATATATTAGTGATTTTAGCTGTAGAAGCTGAACACAAGGAGGAGGAAGAATCTGAGGAAGGATCCGAACAAGAATCGGGAGATGAAACTGATGATGAGGATAAACCTAGAAGGAAAGGCACTCAAGGTATTATTGAGATTGAAAATCCTAACTTGGTGAAGCCAAAAACCTTGAAAGCTAGAGATATTGATATTGGGAAAACAACTGAACTTTCAAGGCGTGAAAGAGAGGAGATAGAGAAACAAAAGGCTCATGAGCGATACATGAGGCTTCAAGAACAAGGGAAAACTGAACAAGCTAGGAAAGATTTAGAACGTTTGTCTCTTATTCGTCAACAAAGGGCAGAAGCTGCTAAAAAAAGAGAAGAAGAAAAGGCTGCCAAAGAACAGAAGAAAGCAGAAGCTCGCAAATGAGCTCCGGGACCCTCCGAGTTCTTTTCTCTGGGACGAAAGACATGTAATCTCTACAATTTTACTTTCTAGTTTGCCTAGACTAATATTGTCAGCAACCAAGTTATATTCTCTTATTTGGCACAGAAGTGACCATAGTATTAAGCCGTTTTACATTTATAATTTACGCAAGTATACTTCTTATATCTCTACGGCATAAGAGGTTTGATTCAATATGACTTTAATATTAGGTTATAACCTTTATTCTCTACGCCTTTATACCTTATCAATTTAGAATATGTTTCCATTTTCTTCTTTAAATATTGGCCTAGAATACTTCTGAGA

Coding sequence (CDS)

ATGTTCCATGGTTTGGGAATCCGGTTGGCGATTTGCACCGCAGAGAGATGTAAACGGGACGGGAACAAAGAAGCCAACACAACTATAAATTCACTTCTCGGCGGAAAGGCGGAGAAGTCACTAACTTCGTCTTCCACAACTAAAACTAAGAGGGGACTTCTATTCTACGTCGATTCTGCTTCTCTTCTGCTTCTTCGTCCGATTCTACCTTCTCCCTGCTGCTTTTCGTTGACGATGGGAAGAGGAAAGTACAAGAGCAAGCCCACCGGTCGGCGCCAATTCTCTACCCCTGAAGACATGCGATACGAGATGGTAGTGGACATGGAGAGTCCAGTAGTTGGAATGATGCTGGATATATTAGTGATTTTAGCTGTAGAAGCTGAACACAAGGAGGAGGAAGAATCTGAGGAAGGATCCGAACAAGAATCGGGAGATGAAACTGATGATGAGGATAAACCTAGAAGGAAAGGCACTCAAGGTATTATTGAGATTGAAAATCCTAACTTGGTGAAGCCAAAAACCTTGAAAGCTAGAGATATTGATATTGGGAAAACAACTGAACTTTCAAGGCGTGAAAGAGAGGAGATAGAGAAACAAAAGGCTCATGAGCGATACATGAGGCTTCAAGAACAAGGGAAAACTGAACAAGCTAGGAAAGATTTAGAACGTTTGTCTCTTATTCGTCAACAAAGGGCAGAAGCTGCTAAAAAAAGAGAAGAAGAAAAGGCTGCCAAAGAACAGAAGAAAGCAGAAGCTCGCAAATGA

Protein sequence

MFHGLGIRLAICTAERCKRDGNKEANTTINSLLGGKAEKSLTSSSTTKTKRGLLFYVDSASLLLLRPILPSPCCFSLTMGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEEGSEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK
Homology
BLAST of Carg15322 vs. NCBI nr
Match: KAG7034622.1 (28 kDa heat- and acid-stable phosphoprotein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 459.5 bits (1181), Expect = 1.8e-125
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0

Query: 1   MFHGLGIRLAICTAERCKRDGNKEANTTINSLLGGKAEKSLTSSSTTKTKRGLLFYVDSA 60
           MFHGLGIRLAICTAERCKRDGNKEANTTINSLLGGKAEKSLTSSSTTKTKRGLLFYVDSA
Sbjct: 1   MFHGLGIRLAICTAERCKRDGNKEANTTINSLLGGKAEKSLTSSSTTKTKRGLLFYVDSA 60

Query: 61  SLLLLRPILPSPCCFSLTMGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDIL 120
           SLLLLRPILPSPCCFSLTMGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDIL
Sbjct: 61  SLLLLRPILPSPCCFSLTMGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDIL 120

Query: 121 VILAVEAEHKEEEESEEGSEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDI 180
           VILAVEAEHKEEEESEEGSEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDI
Sbjct: 121 VILAVEAEHKEEEESEEGSEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDI 180

Query: 181 DIGKTTELSRREREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREE 240
           DIGKTTELSRREREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREE
Sbjct: 181 DIGKTTELSRREREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREE 240

Query: 241 EKAAKEQKKAEARK 255
           EKAAKEQKKAEARK
Sbjct: 241 EKAAKEQKKAEARK 254

BLAST of Carg15322 vs. NCBI nr
Match: KAG6581338.1 (28 kDa heat- and acid-stable phosphoprotein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 397.5 bits (1020), Expect = 8.6e-107
Identity = 228/254 (89.76%), Postives = 231/254 (90.94%), Query Frame = 0

Query: 1   MFHGLGIRLAICTAERCKRDGNKEANTTINSLLGGKAEKSLTSSSTTKTKRGLLFYVDSA 60
           M +GLGIRLAICTA+RCKRDGNKEANTTINSLLGGKAEKSLTSSSTTKTKRGLLFYVDSA
Sbjct: 1   MENGLGIRLAICTADRCKRDGNKEANTTINSLLGGKAEKSLTSSSTTKTKRGLLFYVDSA 60

Query: 61  SLLLLRPILPSPCCFSLTMGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDIL 120
           SLLLLRPILPSPCCFSLTMGRGKYKSKPTGRRQFSTPEDM           + G      
Sbjct: 61  SLLLLRPILPSPCCFSLTMGRGKYKSKPTGRRQFSTPEDM-----------LAGTSTRPR 120

Query: 121 VILAVEAEHKEEEESEEGSEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDI 180
               VEAEHKEEEESEEGSEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDI
Sbjct: 121 TFKQVEAEHKEEEESEEGSEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDI 180

Query: 181 DIGKTTELSRREREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREE 240
           DIGKTTELSRREREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREE
Sbjct: 181 DIGKTTELSRREREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREE 240

Query: 241 EKAAKEQKKAEARK 255
           EKAAKEQKKAEARK
Sbjct: 241 EKAAKEQKKAEARK 243

BLAST of Carg15322 vs. NCBI nr
Match: XP_022925842.1 (28 kDa heat- and acid-stable phosphoprotein-like [Cucurbita moschata] >XP_022978774.1 28 kDa heat- and acid-stable phosphoprotein-like [Cucurbita maxima])

HSP 1 Score: 253.4 bits (646), Expect = 2.0e-63
Identity = 153/176 (86.93%), Postives = 154/176 (87.50%), Query Frame = 0

Query: 79  MGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEEG 138
           MGRGKYKSKPTGRRQFSTPEDM           + G          VEAEHKEEEESEEG
Sbjct: 1   MGRGKYKSKPTGRRQFSTPEDM-----------LAGTSTRPRTFKQVEAEHKEEEESEEG 60

Query: 139 SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK 198
           SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK
Sbjct: 61  SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK 120

Query: 199 QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 255
           QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK
Sbjct: 121 QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 165

BLAST of Carg15322 vs. NCBI nr
Match: XP_023543121.1 (28 kDa heat- and acid-stable phosphoprotein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 252.3 bits (643), Expect = 4.5e-63
Identity = 152/176 (86.36%), Postives = 154/176 (87.50%), Query Frame = 0

Query: 79  MGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEEG 138
           MGRGKYKSKPTGRRQFSTPEDM           + G          VEAEHKEEEESEEG
Sbjct: 1   MGRGKYKSKPTGRRQFSTPEDM-----------LAGTSTRPRTFKQVEAEHKEEEESEEG 60

Query: 139 SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK 198
           S+QESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK
Sbjct: 61  SDQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK 120

Query: 199 QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 255
           QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK
Sbjct: 121 QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 165

BLAST of Carg15322 vs. NCBI nr
Match: XP_038883580.1 (28 kDa heat- and acid-stable phosphoprotein [Benincasa hispida])

HSP 1 Score: 243.4 bits (620), Expect = 2.1e-60
Identity = 149/177 (84.18%), Postives = 154/177 (87.01%), Query Frame = 0

Query: 79  MGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEEG 138
           MGRGKYKSKPTGRRQFSTPEDM           + G          VEAEHKEEEESEEG
Sbjct: 1   MGRGKYKSKPTGRRQFSTPEDM-----------LAGTSTRPRTFKQVEAEHKEEEESEEG 60

Query: 139 SEQESGDETDDE-DKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE 198
           SEQESGDE+DD+ DKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE
Sbjct: 61  SEQESGDESDDQGDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE 120

Query: 199 KQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 255
           KQKAHER+MRLQEQGKTEQARKDLERL+LIRQQRAEAAKKREEEKAAKEQKKAEARK
Sbjct: 121 KQKAHERFMRLQEQGKTEQARKDLERLTLIRQQRAEAAKKREEEKAAKEQKKAEARK 166

BLAST of Carg15322 vs. ExPASy Swiss-Prot
Match: Q3UHX2 (28 kDa heat- and acid-stable phosphoprotein OS=Mus musculus OX=10090 GN=Pdap1 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.1e-19
Identity = 78/191 (40.84%), Postives = 112/191 (58.64%), Query Frame = 0

Query: 79  MGRGKYKSKPTGR-RQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEE 138
           M +G  K    GR RQ+++PE++  ++  + +                  +  EE+E EE
Sbjct: 1   MPKGGRKGGHKGRVRQYTSPEEIDAQLQAEKQ------------------KANEEDEQEE 60

Query: 139 GSEQESG-----------DETDDED---KPRRKGTQGIIEIENPNLVKPKTLKARDIDIG 198
           G +  SG           DE++DED   + +RKG +G+I+IENPN V   T K   +D+ 
Sbjct: 61  GGDGASGDPKKEKKSLDSDESEDEDDDYQQKRKGVEGLIDIENPNRVAQTTKKVTQLDLD 120

Query: 199 KTTELSRREREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKA 255
              ELSRREREEIEKQKA ERYM++   GKTEQA+ DL RL++IR+QR EAA+K+EEE+ 
Sbjct: 121 GPKELSRREREEIEKQKAKERYMKMHLAGKTEQAKADLARLAIIRKQREEAARKKEEERK 173

BLAST of Carg15322 vs. ExPASy Swiss-Prot
Match: Q62785 (28 kDa heat- and acid-stable phosphoprotein OS=Rattus norvegicus OX=10116 GN=Pdap1 PE=1 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 3.1e-19
Identity = 78/191 (40.84%), Postives = 112/191 (58.64%), Query Frame = 0

Query: 79  MGRGKYKSKPTGR-RQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEE 138
           M +G  K    GR RQ+++PE++  ++  + +                  +  EE+E EE
Sbjct: 1   MPKGGRKGGHKGRVRQYTSPEEIDAQLQAEKQ------------------KANEEDEQEE 60

Query: 139 GSEQESG-----------DETDDED---KPRRKGTQGIIEIENPNLVKPKTLKARDIDIG 198
           G +  SG           DE++DED   + +RKG +G+I+IENPN V   T K   +D+ 
Sbjct: 61  GGDGASGDPKKEKKSLDSDESEDEDDDYQQKRKGVEGLIDIENPNRVAQTTKKVTQLDLD 120

Query: 199 KTTELSRREREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKA 255
              ELSRREREEIEKQKA ERYM++   GKTEQA+ DL RL++IR+QR EAA+K+EEE+ 
Sbjct: 121 GPKELSRREREEIEKQKAKERYMKMHLAGKTEQAKADLARLAIIRKQREEAARKKEEERK 173

BLAST of Carg15322 vs. ExPASy Swiss-Prot
Match: Q13442 (28 kDa heat- and acid-stable phosphoprotein OS=Homo sapiens OX=9606 GN=PDAP1 PE=1 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.6e-18
Identity = 76/191 (39.79%), Postives = 113/191 (59.16%), Query Frame = 0

Query: 79  MGRGKYKSKPTGR-RQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEE 138
           M +G  K    GR RQ+++PE++  ++  + +                  + +EEEE +E
Sbjct: 1   MPKGGRKGGHKGRARQYTSPEEIDAQLQAEKQ------------------KAREEEEQKE 60

Query: 139 GSEQESG-----------DETDDED---KPRRKGTQGIIEIENPNLVKPKTLKARDIDIG 198
           G +  +G           DE++DE+   + +RKG +G+I+IENPN V   T K   +D+ 
Sbjct: 61  GGDGAAGDPKKEKKSLDSDESEDEEDDYQQKRKGVEGLIDIENPNRVAQTTKKVTQLDLD 120

Query: 199 KTTELSRREREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKA 255
              ELSRREREEIEKQKA ERYM++   GKTEQA+ DL RL++IR+QR EAA+K+EEE+ 
Sbjct: 121 GPKELSRREREEIEKQKAKERYMKMHLAGKTEQAKADLARLAIIRKQREEAARKKEEERK 173

BLAST of Carg15322 vs. ExPASy Swiss-Prot
Match: Q556U5 (28 kDa heat- and acid-stable phosphoprotein homolog OS=Dictyostelium discoideum OX=44689 GN=DDB_G0273095 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 9.5e-08
Identity = 52/123 (42.28%), Postives = 73/123 (59.35%), Query Frame = 0

Query: 132 EEESEEGSEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRR 191
           +++ E  SE +S  E+D ED           EI NPN +K  T K  +I++    ELSRR
Sbjct: 100 KKQQESDSEDDSDKESDSED-----------EIANPNRMKQVTKKLSEINVNAKVELSRR 159

Query: 192 EREEIEKQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAE 251
           E+EE+ +Q A +R          +Q + DLERL +IR+QR EAAK++EEEK A E+K AE
Sbjct: 160 EKEELARQAATQRQ-------NEKQQKSDLERLQVIRKQREEAAKRKEEEKKANEEKMAE 204

Query: 252 ARK 255
            R+
Sbjct: 220 RRR 204

BLAST of Carg15322 vs. ExPASy TrEMBL
Match: A0A6J1IUZ4 (28 kDa heat- and acid-stable phosphoprotein-like OS=Cucurbita maxima OX=3661 GN=LOC111478634 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 9.7e-64
Identity = 153/176 (86.93%), Postives = 154/176 (87.50%), Query Frame = 0

Query: 79  MGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEEG 138
           MGRGKYKSKPTGRRQFSTPEDM           + G          VEAEHKEEEESEEG
Sbjct: 1   MGRGKYKSKPTGRRQFSTPEDM-----------LAGTSTRPRTFKQVEAEHKEEEESEEG 60

Query: 139 SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK 198
           SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK
Sbjct: 61  SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK 120

Query: 199 QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 255
           QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK
Sbjct: 121 QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 165

BLAST of Carg15322 vs. ExPASy TrEMBL
Match: A0A6J1ECQ7 (28 kDa heat- and acid-stable phosphoprotein-like OS=Cucurbita moschata OX=3662 GN=LOC111433129 PE=4 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 9.7e-64
Identity = 153/176 (86.93%), Postives = 154/176 (87.50%), Query Frame = 0

Query: 79  MGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEEG 138
           MGRGKYKSKPTGRRQFSTPEDM           + G          VEAEHKEEEESEEG
Sbjct: 1   MGRGKYKSKPTGRRQFSTPEDM-----------LAGTSTRPRTFKQVEAEHKEEEESEEG 60

Query: 139 SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK 198
           SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK
Sbjct: 61  SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK 120

Query: 199 QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 255
           QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK
Sbjct: 121 QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 165

BLAST of Carg15322 vs. ExPASy TrEMBL
Match: A0A5D3CQ74 (28 kDa heat-and acid-stable phosphoprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007860 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 3.8e-60
Identity = 148/177 (83.62%), Postives = 153/177 (86.44%), Query Frame = 0

Query: 79  MGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEEG 138
           MGRGKYKSKPTGRRQFSTPEDM           + G          VEAEHKEEEESEEG
Sbjct: 1   MGRGKYKSKPTGRRQFSTPEDM-----------LAGTSTRPRTFKQVEAEHKEEEESEEG 60

Query: 139 SEQESGDETDDE-DKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE 198
           SEQESGDE+DD+ DKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE
Sbjct: 61  SEQESGDESDDQGDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE 120

Query: 199 KQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 255
           KQKAHER+MRLQEQGKTEQARKDLERLSLIRQQR EAAKKREEEKAA+EQKKAEARK
Sbjct: 121 KQKAHERFMRLQEQGKTEQARKDLERLSLIRQQREEAAKKREEEKAAREQKKAEARK 166

BLAST of Carg15322 vs. ExPASy TrEMBL
Match: A0A1S3AZ63 (28 kDa heat- and acid-stable phosphoprotein OS=Cucumis melo OX=3656 GN=LOC103484483 PE=4 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 3.8e-60
Identity = 148/177 (83.62%), Postives = 153/177 (86.44%), Query Frame = 0

Query: 79  MGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEEG 138
           MGRGKYKSKPTGRRQFSTPEDM           + G          VEAEHKEEEESEEG
Sbjct: 1   MGRGKYKSKPTGRRQFSTPEDM-----------LAGTSTRPRTFKQVEAEHKEEEESEEG 60

Query: 139 SEQESGDETDDE-DKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE 198
           SEQESGDE+DD+ DKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE
Sbjct: 61  SEQESGDESDDQGDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE 120

Query: 199 KQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 255
           KQKAHER+MRLQEQGKTEQARKDLERLSLIRQQR EAAKKREEEKAA+EQKKAEARK
Sbjct: 121 KQKAHERFMRLQEQGKTEQARKDLERLSLIRQQREEAAKKREEEKAAREQKKAEARK 166

BLAST of Carg15322 vs. ExPASy TrEMBL
Match: A0A0A0KHS1 (PP28 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G518250 PE=4 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 8.5e-60
Identity = 147/177 (83.05%), Postives = 153/177 (86.44%), Query Frame = 0

Query: 79  MGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEEG 138
           MGRGKYKSKPTGRRQFSTPEDM           + G          VEAEHKEEE+SEEG
Sbjct: 1   MGRGKYKSKPTGRRQFSTPEDM-----------LAGTSTRPRTFKQVEAEHKEEEDSEEG 60

Query: 139 SEQESGDETDDE-DKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE 198
           SEQESGDE+DD+ DKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE
Sbjct: 61  SEQESGDESDDQGDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIE 120

Query: 199 KQKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 255
           KQKAHER+MRLQEQGKTEQARKDLERLSLIRQQR EAAKKREEEKAA+EQKKAEARK
Sbjct: 121 KQKAHERFMRLQEQGKTEQARKDLERLSLIRQQREEAAKKREEEKAAREQKKAEARK 166

BLAST of Carg15322 vs. TAIR 10
Match: AT5G46020.1 (CONTAINS InterPro DOMAIN/s: Casein kinase substrate, phosphoprotein PP28 (InterPro:IPR019380); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 161.0 bits (406), Expect = 1.3e-39
Identity = 102/176 (57.95%), Postives = 129/176 (73.30%), Query Frame = 0

Query: 79  MGRGKYKSKPTGRRQFSTPEDMRYEMVVDMESPVVGMMLDILVILAVEAEHKEEEESEEG 138
           MGRGK+K KPTG+R+FS+  D+                         + E + EE+ EE 
Sbjct: 1   MGRGKFKGKPTGQRRFSSAADILAGTSAARPRSF------------KQKEAEYEEDVEEE 60

Query: 139 SEQESGDETDDEDKPRRKGTQGIIEIENPNLVKPKTLKARDIDIGKTTELSRREREEIEK 198
           SE+ES +E++DE   ++KG + +IE++NPN V+ KTLKA+D+D  KTTELSRREREE+EK
Sbjct: 61  SEEESEEESEDEADVKKKGAEAVIEVDNPNRVRQKTLKAKDLDASKTTELSRREREELEK 120

Query: 199 QKAHERYMRLQEQGKTEQARKDLERLSLIRQQRAEAAKKREEEKAAKEQKKAEARK 255
           Q+AHERYMRLQEQGKTEQARKDL+RL+LIRQQR EAAKKREEEKAA++ KK E RK
Sbjct: 121 QRAHERYMRLQEQGKTEQARKDLDRLALIRQQREEAAKKREEEKAARDAKKVEGRK 164

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7034622.11.8e-125100.0028 kDa heat- and acid-stable phosphoprotein, partial [Cucurbita argyrosperma sub... [more]
KAG6581338.18.6e-10789.7628 kDa heat- and acid-stable phosphoprotein, partial [Cucurbita argyrosperma sub... [more]
XP_022925842.12.0e-6386.9328 kDa heat- and acid-stable phosphoprotein-like [Cucurbita moschata] >XP_022978... [more]
XP_023543121.14.5e-6386.3628 kDa heat- and acid-stable phosphoprotein-like [Cucurbita pepo subsp. pepo][more]
XP_038883580.12.1e-6084.1828 kDa heat- and acid-stable phosphoprotein [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q3UHX23.1e-1940.8428 kDa heat- and acid-stable phosphoprotein OS=Mus musculus OX=10090 GN=Pdap1 PE... [more]
Q627853.1e-1940.8428 kDa heat- and acid-stable phosphoprotein OS=Rattus norvegicus OX=10116 GN=Pda... [more]
Q134421.6e-1839.7928 kDa heat- and acid-stable phosphoprotein OS=Homo sapiens OX=9606 GN=PDAP1 PE=... [more]
Q556U59.5e-0842.2828 kDa heat- and acid-stable phosphoprotein homolog OS=Dictyostelium discoideum ... [more]
Match NameE-valueIdentityDescription
A0A6J1IUZ49.7e-6486.9328 kDa heat- and acid-stable phosphoprotein-like OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1ECQ79.7e-6486.9328 kDa heat- and acid-stable phosphoprotein-like OS=Cucurbita moschata OX=3662 G... [more]
A0A5D3CQ743.8e-6083.6228 kDa heat-and acid-stable phosphoprotein OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3AZ633.8e-6083.6228 kDa heat- and acid-stable phosphoprotein OS=Cucumis melo OX=3656 GN=LOC103484... [more]
A0A0A0KHS18.5e-6083.05PP28 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G518250 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT5G46020.11.3e-3957.95CONTAINS InterPro DOMAIN/s: Casein kinase substrate, phosphoprotein PP28 (InterP... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 191..211
NoneNo IPR availableCOILSCoilCoilcoord: 227..254
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 130..147
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 172..254
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 129..254
NoneNo IPR availablePANTHERPTHR22055:SF828 KDA HEAT- AND ACID-STABLE PHOSPHOPROTEIN-LIKE ISOFORM X1coord: 79..101
coord: 129..254
IPR019380Casein kinase substrate, phosphoprotein PP28PFAMPF10252PP28coord: 166..243
e-value: 7.1E-27
score: 93.7
IPR03987628kDa heat- and acid-stable phosphoproteinPANTHERPTHR2205528 KDA HEAT- AND ACID-STABLE PHOSPHOPROTEIN PDGF-ASSOCIATED PROTEINcoord: 79..101
coord: 129..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg15322-RACarg15322-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
molecular_function GO:0016301 kinase activity