Carg14784 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg14784
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cytochrome b561 domain-containing protein
Location	Carg_Chr01: 10398634 .. 10401055 (-)
RNA-Seq Expression	Carg14784
Synteny	Carg14784

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTATTTTTACCGAACAAAAAGTCCTCTTTCTCCATTCTTTTTCTCCTTCTTCTTCTCACATTTCAACCACTCAGTTCATCCCAACAGCTCAAGAAAAATGTAGAATTCAATACAAGCAAGAAAGATCACAGCCAGAAGGTTTCTTATCGTTCTTCCTATTGTTTCATTTTCATATTTGAAATGAATATCATTGCAGAAAAAGAAGGTTTAAATGTATAGCTGAAAGTGATTATTGGTTAATAATGGCAGCAGATTAGTTCCAGCTTACTGTTTGATATCACACTCCATGGATTTCTTCTATGGGCTTCAATGGGGTTCCTTATGCCGGTTGGGATTCTTGTTATCAGAATGTCCAACAGAGAGCAATGTGGAAGAAGGCTGAAGTTTTACTTTTACATTCACACAATTCTACAGGCAAGTTCATCTCTGGTTCATCTCAATCTTCTCTAGCAATAATGAAATTCTGTTCGATAGCTATTTTGGTTTTTGAAAGTTATATAACGGCTCAAGCCCACCGCTAGTAGATATTGTCCTCTTTAGGCTTTCCCTTTCGGGCTTTTCCTCAAGGTTTTTAAAACGCGTCTGCTAGGAAAAGGTTTCCACGCCCTTATAAAGAATATTTTGTTTTCTCCCCAACCGATGTGGGATCTCACAATCCACCCCCCTTCAGGGCCCAACATCTTCGCTGGCACTCGTTCCCTTCTCCAATCGATGTGGGACCCTCCCAATCTACCCCTTTTTTTGGGCCAGCGTCCTTGCTGGCATACTGCCTCGTGTCCACCCCCCTTGGGGATCAGCCTCCTCGTTAACACATCACTCGATGTCTGGCTCTAATACCATTTAACTTACCTAAGCCCACCACTAGTAGATATTGTCCTCTTTAGGCTTTCCCTTTCAAGCTTCCCCTCAAGGTTTCTAAAACGCGCCTGTTAGGGAGAGGTTTCCACACCCTTGTAAAGAATGTTTCGTTTCCTCCCCAACCGACATGGGATCTCACACATTACTTCCACCTAAATGTTTCTTTCTTTATTCAAAAACTAAAAGATTCTGAAAAAACTTGCTTTCGTTTTTTAAACTTGGGTAAGAATTCTATTGTTTCTTCGCTAAGGAAAGTGAAAACCATAGTGAATCCTTTGATCATCAAATGGGGCCTAAAATAAACCCTATGGTGTTTGTTTTCTTAAAGAAGAATCCTTTTAAACTAAACAAACAATAACTACATAGAGTTGCACGAAAGCATCATCATTAATGCAATTAGCTTTATCTTTTCACTAAACAAGCAAACAATCCACTTTTTTCTTGACTTTATGAGCTTAATGATGGTTCAAGAGAAATCCCAAGGGCCCTGATTCTGAATATCTTATACAGCCCCCAAGTTTCTAAACACAAAAAGTTGATCCAGCAGAATATTATGAATACTTGCATCGAAAGAAAAGACAGGCATCATCACATTCTAACTAATTAGATACTCAAATAGAAACTAAGTTAAAATTCCAAATTTATAGTAGCAGTTTGATGGATTTTGATGATCCTTTAATCACAAAGATGGGTTCATTACATTATCTTAACTTGATTATGATGAACAGATACTCTCTGTATTACTAGTCACAGCTGGAGCAGTCATGTCTATCAAGAAATTCAACAATGCTTTCAATAATAACCATCAAAGGATAGGGATTGGCCTATATGGGATGATCTGGTTACAGGCATTGATTGGAATTGTAAGGCCAAAAAGGTAAAGTTTCATATTTTCTTCAAATATGCAGCAATAGTTTATTAGAAAGAGTTATTGTGAGGTTGTTTTCAAAGTAGTACTGTAACAGCCCAAACTCACCGCTAGCAGATATTGTTTGCTATACCTTGTTACATATCGTCGTCAGCCTCACAGTTTTAAAACGTGTCTGCTAGGGAGAGGTTTCCACATCCTTATAAAGAATACTTTTTTCCTCTCTCCAACTGATGTGGGACCCCGTAATTCATTCCTTTCCGTGGATTTAGATAACAAAACAAAGGAAGTAGTGTTCAGTGGATTTAGATCAGGAAAGAGGAATAGTATGATTTATTTCAATTTGTATACTCTGAATCCATTCCTTTCTCATATTCTGGCAGGGGCTCTAGAACAAGAAGCCTATGGTTTTTCATACACTGGATGCTTGGCACTGCAGTATCTCTCCTAGGAGTTTTCAATGTATACTCAGGATTATTTGCTTACCACGAGAAAACATCACGAAGGATACGAATTTGGACCATAATTTTCACTGTTGAAATTTCTTTGATTTCCTTCTTTTACCTATTCCAAGACAAACTGGAGTATATACAAAAGCAAGGAGTTATTTTGGGCAGTGATTTGGTAACACCTACTGATCAAGGGCTCTCGCCAAATGACAAGCAAAAGGAACTGCCTACTGATCCATGCTGA

mRNA sequence

ATGCTATTTTTACCGAACAAAAAGTCCTCTTTCTCCATTCTTTTTCTCCTTCTTCTTCTCACATTTCAACCACTCAGTTCATCCCAACAGCTCAAGAAAAATGTAGAATTCAATACAAGCAAGAAAGATCACAGCCAGAAGATTAGTTCCAGCTTACTGTTTGATATCACACTCCATGGATTTCTTCTATGGGCTTCAATGGGGTTCCTTATGCCGGTTGGGATTCTTGTTATCAGAATGTCCAACAGAGAGCAATGTGGAAGAAGGCTGAAGGGCTCTAGAACAAGAAGCCTATGGTTTTTCATACACTGGATGCTTGGCACTGCAGTATCTCTCCTAGGAGTTTTCAATGTATACTCAGGATTATTTGCTTACCACGAGAAAACATCACGAAGGATACGAATTTGGACCATAATTTTCACTGTTGAAATTTCTTTGATTTCCTTCTTTTACCTATTCCAAGACAAACTGGAGTATATACAAAAGCAAGGAGTTATTTTGGGCAGTGATTTGGTAACACCTACTGATCAAGGGCTCTCGCCAAATGACAAGCAAAAGGAACTGCCTACTGATCCATGCTGA

Coding sequence (CDS)

ATGCTATTTTTACCGAACAAAAAGTCCTCTTTCTCCATTCTTTTTCTCCTTCTTCTTCTCACATTTCAACCACTCAGTTCATCCCAACAGCTCAAGAAAAATGTAGAATTCAATACAAGCAAGAAAGATCACAGCCAGAAGATTAGTTCCAGCTTACTGTTTGATATCACACTCCATGGATTTCTTCTATGGGCTTCAATGGGGTTCCTTATGCCGGTTGGGATTCTTGTTATCAGAATGTCCAACAGAGAGCAATGTGGAAGAAGGCTGAAGGGCTCTAGAACAAGAAGCCTATGGTTTTTCATACACTGGATGCTTGGCACTGCAGTATCTCTCCTAGGAGTTTTCAATGTATACTCAGGATTATTTGCTTACCACGAGAAAACATCACGAAGGATACGAATTTGGACCATAATTTTCACTGTTGAAATTTCTTTGATTTCCTTCTTTTACCTATTCCAAGACAAACTGGAGTATATACAAAAGCAAGGAGTTATTTTGGGCAGTGATTTGGTAACACCTACTGATCAAGGGCTCTCGCCAAATGACAAGCAAAAGGAACTGCCTACTGATCCATGCTGA

Protein sequence

MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHGFLLWASMGFLMPVGILVIRMSNREQCGRRLKGSRTRSLWFFIHWMLGTAVSLLGVFNVYSGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLSPNDKQKELPTDPC

Homology

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BLAST of Carg14784 vs. NCBI nr
Match: KAG7037492.1 (Cytochrome b561 domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 379.0 bits (972), Expect = 2.4e-101
Identity = 193/193 (100.00%), Postives = 193/193 (100.00%), Query Frame = 0


Query: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG 60
           MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG
Sbjct: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG 60

Query: 61  FLLWASMGFLMPVGILVIRMSNREQCGRRLKGSRTRSLWFFIHWMLGTAVSLLGVFNVYS 120
           FLLWASMGFLMPVGILVIRMSNREQCGRRLKGSRTRSLWFFIHWMLGTAVSLLGVFNVYS
Sbjct: 61  FLLWASMGFLMPVGILVIRMSNREQCGRRLKGSRTRSLWFFIHWMLGTAVSLLGVFNVYS 120

Query: 121 GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS 180
           GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS
Sbjct: 121 GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS 180

Query: 181 PNDKQKELPTDPC 194
           PNDKQKELPTDPC
Sbjct: 181 PNDKQKELPTDPC 193

[Close]

BLAST of Carg14784 vs. NCBI nr
Match: XP_022940301.1 (cytochrome b561 domain-containing protein At4g18260-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 348.6 bits (893), Expect = 3.5e-92
Identity = 192/253 (75.89%), Postives = 192/253 (75.89%), Query Frame = 0


Query: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG 60
           MLFL NKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG
Sbjct: 1   MLFLQNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG 60

Query: 61  FLLWASMGFLMPVGILVIRMSNREQCGRRLK----------------------------- 120
           FLLWASMGFLMPVGILVIRMSNREQCGRRLK                             
Sbjct: 61  FLLWASMGFLMPVGILVIRMSNREQCGRRLKFYFYIHTILQILSVLLVTAGAVMSIKKFN 120

Query: 121 -------------------------------GSRTRSLWFFIHWMLGTAVSLLGVFNVYS 180
                                          GSRTRSLWFFIHWMLGTAVSLLGVFNVYS
Sbjct: 121 NAFNNNHQRIGIGLYGMIWLQALIGIVRPKRGSRTRSLWFFIHWMLGTAVSLLGVFNVYS 180

Query: 181 GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS 194
           GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS
Sbjct: 181 GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS 240

[Close]

BLAST of Carg14784 vs. NCBI nr
Match: KAG6607978.1 (Cytochrome b561 domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 347.1 bits (889), Expect = 1.0e-91
Identity = 193/254 (75.98%), Postives = 193/254 (75.98%), Query Frame = 0


Query: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQK-ISSSLLFDITLH 60
           MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQK ISSSLLFDITLH
Sbjct: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKQISSSLLFDITLH 60

Query: 61  GFLLWASMGFLMPVGILVIRMSNREQCGRRLK---------------------------- 120
           GFLLWASMGFLMPVGILVIRMSNREQCGRRLK                            
Sbjct: 61  GFLLWASMGFLMPVGILVIRMSNREQCGRRLKFYFYIHTILQILSVLLVTAGAVMSIKKF 120

Query: 121 --------------------------------GSRTRSLWFFIHWMLGTAVSLLGVFNVY 180
                                           GSRTRSLWFFIHWMLGTAVSLLGVFNVY
Sbjct: 121 NNAFNNNHQRIGIGLYGMIWLQALIGIVRPKRGSRTRSLWFFIHWMLGTAVSLLGVFNVY 180

Query: 181 SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGL 194
           SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGL
Sbjct: 181 SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGL 240

[Close]

BLAST of Carg14784 vs. NCBI nr
Match: XP_022940300.1 (cytochrome b561 domain-containing protein At4g18260-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 344.0 bits (881), Expect = 8.6e-91
Identity = 192/254 (75.59%), Postives = 192/254 (75.59%), Query Frame = 0


Query: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQK-ISSSLLFDITLH 60
           MLFL NKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQK ISSSLLFDITLH
Sbjct: 1   MLFLQNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKQISSSLLFDITLH 60

Query: 61  GFLLWASMGFLMPVGILVIRMSNREQCGRRLK---------------------------- 120
           GFLLWASMGFLMPVGILVIRMSNREQCGRRLK                            
Sbjct: 61  GFLLWASMGFLMPVGILVIRMSNREQCGRRLKFYFYIHTILQILSVLLVTAGAVMSIKKF 120

Query: 121 --------------------------------GSRTRSLWFFIHWMLGTAVSLLGVFNVY 180
                                           GSRTRSLWFFIHWMLGTAVSLLGVFNVY
Sbjct: 121 NNAFNNNHQRIGIGLYGMIWLQALIGIVRPKRGSRTRSLWFFIHWMLGTAVSLLGVFNVY 180

Query: 181 SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGL 194
           SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGL
Sbjct: 181 SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGL 240

[Close]

BLAST of Carg14784 vs. NCBI nr
Match: XP_022981110.1 (cytochrome b561 domain-containing protein At4g18260-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 340.1 bits (871), Expect = 1.2e-89
Identity = 188/253 (74.31%), Postives = 189/253 (74.70%), Query Frame = 0


Query: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG 60
           MLFL NKK SFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDH QKIS+SLLFDITLHG
Sbjct: 1   MLFLQNKKFSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHCQKISASLLFDITLHG 60

Query: 61  FLLWASMGFLMPVGILVIRMSNREQCGRRLK----------------------------- 120
           FLLWASMGFLMPVGILVIRMSNREQCGRRLK                             
Sbjct: 61  FLLWASMGFLMPVGILVIRMSNREQCGRRLKFYFYIHTILQILSVLLVTAGAVMSIKKFN 120

Query: 121 -------------------------------GSRTRSLWFFIHWMLGTAVSLLGVFNVYS 180
                                          GSRTRSLWFFIHWMLGTAVSLLGVFNVYS
Sbjct: 121 NAFNNNHQRIGIGLYGMIWLQALIGIVRPKRGSRTRSLWFFIHWMLGTAVSLLGVFNVYS 180

Query: 181 GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS 194
           GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVT TDQGLS
Sbjct: 181 GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTSTDQGLS 240

[Close]

BLAST of Carg14784 vs. ExPASy Swiss-Prot
Match: Q0WPS2 (Cytochrome b561 domain-containing protein At4g18260 OS=Arabidopsis thaliana OX=3702 GN=At4g18260 PE=2 SV=1)

HSP 1 Score: 110.2 bits (274), Expect = 2.7e-23
Identity = 75/231 (32.47%), Postives = 110/231 (47.62%), Query Frame = 0


Query: 8   KSSFSILFLLL--LLTFQPLSSSQQLKKNVE------FNTSKKDHSQKISSSLLFDITLH 67
           KS +S++  +L  L +  P      L+  ++        T       K+S  ++  I LH
Sbjct: 5   KSLWSVMISILFYLTSAPPFVICSSLEVTIDNHSPSNLKTKGSLEQDKLSHQMINSIKLH 64

Query: 68  GFLLWASMGFLMPVGILVIRMSNR------------------------------------ 127
           G LLW SMGFLMPVGIL IRM+N+                                    
Sbjct: 65  GILLWVSMGFLMPVGILFIRMANKAHENGIKVKVFFYLHVIFQILAVVLATIGAILSLRT 124

Query: 128 ---------EQCGRRL----------------KGSRTRSLWFFIHWMLGTAVSLLGVFNV 168
                    ++ G  L                +GS+ R  WF +HW+LGT VS++G+ N+
Sbjct: 125 LENSFDNNHQRLGLALYAAMWLQFLTGVFKPSRGSKRRLRWFLLHWILGTIVSIVGIVNI 184

[Close]

BLAST of Carg14784 vs. ExPASy Swiss-Prot
Match: O80854 (Cytochrome b561 domain-containing protein At2g30890 OS=Arabidopsis thaliana OX=3702 GN=At2g30890 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.6e-23
Identity = 69/217 (31.80%), Postives = 108/217 (49.77%), Query Frame = 0


Query: 12  SILFLLLLLTFQPLSSSQQLKKNVEFNTS----KKDHSQKISSSLLFDITLHGFLLWASM 71
           S+LFLLL     PL SSQ+  +++  + +        S+K++  L+++I +HGF+LWA+M
Sbjct: 10  SLLFLLL-----PLCSSQENTRSLAIDVNGPVETSPISEKLNPKLVYEIKVHGFMLWAAM 69

Query: 72  GFLMPVGILVIRM---------------------------------------------SN 131
           G LMP+GI+ IR+                                             ++
Sbjct: 70  GVLMPIGIISIRLMSIKDQPIITLRRLFFLHVTSQMVAVILVTIGAVMSVINFNNSFSNH 129

Query: 132 REQCG----------------RRLKGSRTRSLWFFIHWMLGTAVSLLGVFNVYSGLFAYH 164
            +Q G                R  +  + R  WF  HW+LGT++++LG+ N+Y+GL AY 
Sbjct: 130 HQQLGIGLYVIVWFQALLGFLRPPREEKARRKWFVGHWILGTSIAILGIINIYTGLHAYA 189

[Close]

BLAST of Carg14784 vs. ExPASy TrEMBL
Match: A0A6J1FI32 (cytochrome b561 domain-containing protein At4g18260-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445961 PE=4 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.7e-92
Identity = 192/253 (75.89%), Postives = 192/253 (75.89%), Query Frame = 0


Query: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG 60
           MLFL NKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG
Sbjct: 1   MLFLQNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG 60

Query: 61  FLLWASMGFLMPVGILVIRMSNREQCGRRLK----------------------------- 120
           FLLWASMGFLMPVGILVIRMSNREQCGRRLK                             
Sbjct: 61  FLLWASMGFLMPVGILVIRMSNREQCGRRLKFYFYIHTILQILSVLLVTAGAVMSIKKFN 120

Query: 121 -------------------------------GSRTRSLWFFIHWMLGTAVSLLGVFNVYS 180
                                          GSRTRSLWFFIHWMLGTAVSLLGVFNVYS
Sbjct: 121 NAFNNNHQRIGIGLYGMIWLQALIGIVRPKRGSRTRSLWFFIHWMLGTAVSLLGVFNVYS 180

Query: 181 GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS 194
           GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS
Sbjct: 181 GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS 240

[Close]

BLAST of Carg14784 vs. ExPASy TrEMBL
Match: A0A6J1FQ60 (cytochrome b561 domain-containing protein At4g18260-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445961 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.1e-91
Identity = 192/254 (75.59%), Postives = 192/254 (75.59%), Query Frame = 0


Query: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQK-ISSSLLFDITLH 60
           MLFL NKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQK ISSSLLFDITLH
Sbjct: 1   MLFLQNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKQISSSLLFDITLH 60

Query: 61  GFLLWASMGFLMPVGILVIRMSNREQCGRRLK---------------------------- 120
           GFLLWASMGFLMPVGILVIRMSNREQCGRRLK                            
Sbjct: 61  GFLLWASMGFLMPVGILVIRMSNREQCGRRLKFYFYIHTILQILSVLLVTAGAVMSIKKF 120

Query: 121 --------------------------------GSRTRSLWFFIHWMLGTAVSLLGVFNVY 180
                                           GSRTRSLWFFIHWMLGTAVSLLGVFNVY
Sbjct: 121 NNAFNNNHQRIGIGLYGMIWLQALIGIVRPKRGSRTRSLWFFIHWMLGTAVSLLGVFNVY 180

Query: 181 SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGL 194
           SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGL
Sbjct: 181 SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGL 240

[Close]

BLAST of Carg14784 vs. ExPASy TrEMBL
Match: A0A6J1IYJ3 (cytochrome b561 domain-containing protein At4g18260-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480357 PE=4 SV=1)

HSP 1 Score: 340.1 bits (871), Expect = 6.0e-90
Identity = 188/253 (74.31%), Postives = 189/253 (74.70%), Query Frame = 0


Query: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFDITLHG 60
           MLFL NKK SFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDH QKIS+SLLFDITLHG
Sbjct: 1   MLFLQNKKFSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHCQKISASLLFDITLHG 60

Query: 61  FLLWASMGFLMPVGILVIRMSNREQCGRRLK----------------------------- 120
           FLLWASMGFLMPVGILVIRMSNREQCGRRLK                             
Sbjct: 61  FLLWASMGFLMPVGILVIRMSNREQCGRRLKFYFYIHTILQILSVLLVTAGAVMSIKKFN 120

Query: 121 -------------------------------GSRTRSLWFFIHWMLGTAVSLLGVFNVYS 180
                                          GSRTRSLWFFIHWMLGTAVSLLGVFNVYS
Sbjct: 121 NAFNNNHQRIGIGLYGMIWLQALIGIVRPKRGSRTRSLWFFIHWMLGTAVSLLGVFNVYS 180

Query: 181 GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGLS 194
           GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVT TDQGLS
Sbjct: 181 GLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTSTDQGLS 240

[Close]

BLAST of Carg14784 vs. ExPASy TrEMBL
Match: A0A6J1J124 (cytochrome b561 domain-containing protein At4g18260-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480357 PE=4 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 1.5e-88
Identity = 188/254 (74.02%), Postives = 189/254 (74.41%), Query Frame = 0


Query: 1   MLFLPNKKSSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQK-ISSSLLFDITLH 60
           MLFL NKK SFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDH QK IS+SLLFDITLH
Sbjct: 1   MLFLQNKKFSFSILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHCQKQISASLLFDITLH 60

Query: 61  GFLLWASMGFLMPVGILVIRMSNREQCGRRLK---------------------------- 120
           GFLLWASMGFLMPVGILVIRMSNREQCGRRLK                            
Sbjct: 61  GFLLWASMGFLMPVGILVIRMSNREQCGRRLKFYFYIHTILQILSVLLVTAGAVMSIKKF 120

Query: 121 --------------------------------GSRTRSLWFFIHWMLGTAVSLLGVFNVY 180
                                           GSRTRSLWFFIHWMLGTAVSLLGVFNVY
Sbjct: 121 NNAFNNNHQRIGIGLYGMIWLQALIGIVRPKRGSRTRSLWFFIHWMLGTAVSLLGVFNVY 180

Query: 181 SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPTDQGL 194
           SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVT TDQGL
Sbjct: 181 SGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTSTDQGL 240

[Close]

BLAST of Carg14784 vs. ExPASy TrEMBL
Match: A0A6J1II03 (cytochrome b561 domain-containing protein At4g18260-like OS=Cucurbita maxima OX=3661 GN=LOC111477093 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 9.6e-80
Identity = 174/258 (67.44%), Postives = 180/258 (69.77%), Query Frame = 0


Query: 1   MLFLPNKKSSF-----SILFLLLLLTFQPLSSSQQLKKNVEFNTSKKDHSQKISSSLLFD 60
           MLFL  +   F     SILFLLLLLTFQP+SSSQQ  KN  FNTSKKDHSQK+SSSLLFD
Sbjct: 1   MLFLQKRMFPFSIPASSILFLLLLLTFQPVSSSQQPYKNENFNTSKKDHSQKMSSSLLFD 60

Query: 61  ITLHGFLLWASMGFLMPVGILVIRMSNREQCGRRLK------------------------ 120
           ITLHGFLLWASMGFLMPVGILVIRMS+REQCGRRLK                        
Sbjct: 61  ITLHGFLLWASMGFLMPVGILVIRMSSREQCGRRLKFYFYIHTILQILSVLLVTAGAVMS 120

Query: 121 ------------------------------------GSRTRSLWFFIHWMLGTAVSLLGV 180
                                               GS TRSLWFFIHWMLGTAVSLLGV
Sbjct: 121 IKKFNNAFNNSHQRIGIGLYGMIWLQALIGIVRPKRGSNTRSLWFFIHWMLGTAVSLLGV 180

Query: 181 FNVYSGLFAYHEKTSRRIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGSDLVTPT 194
           FNVYSGLFAYHEKTSR IRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILG+DL+TPT
Sbjct: 181 FNVYSGLFAYHEKTSRSIRIWTIIFTVEISLISFFYLFQDKLEYIQKQGVILGNDLITPT 240

[Close]

BLAST of Carg14784 vs. TAIR 10
Match: AT4G18260.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 110.2 bits (274), Expect = 1.9e-24
Identity = 75/231 (32.47%), Postives = 110/231 (47.62%), Query Frame = 0


Query: 8   KSSFSILFLLL--LLTFQPLSSSQQLKKNVE------FNTSKKDHSQKISSSLLFDITLH 67
           KS +S++  +L  L +  P      L+  ++        T       K+S  ++  I LH
Sbjct: 5   KSLWSVMISILFYLTSAPPFVICSSLEVTIDNHSPSNLKTKGSLEQDKLSHQMINSIKLH 64

Query: 68  GFLLWASMGFLMPVGILVIRMSNR------------------------------------ 127
           G LLW SMGFLMPVGIL IRM+N+                                    
Sbjct: 65  GILLWVSMGFLMPVGILFIRMANKAHENGIKVKVFFYLHVIFQILAVVLATIGAILSLRT 124

Query: 128 ---------EQCGRRL----------------KGSRTRSLWFFIHWMLGTAVSLLGVFNV 168
                    ++ G  L                +GS+ R  WF +HW+LGT VS++G+ N+
Sbjct: 125 LENSFDNNHQRLGLALYAAMWLQFLTGVFKPSRGSKRRLRWFLLHWILGTIVSIVGIVNI 184

[Close]

BLAST of Carg14784 vs. TAIR 10
Match: AT2G30890.1 (Cytochrome b561/ferric reductase transmembrane protein family )

HSP 1 Score: 109.4 bits (272), Expect = 3.3e-24
Identity = 69/217 (31.80%), Postives = 108/217 (49.77%), Query Frame = 0


Query: 12  SILFLLLLLTFQPLSSSQQLKKNVEFNTS----KKDHSQKISSSLLFDITLHGFLLWASM 71
           S+LFLLL     PL SSQ+  +++  + +        S+K++  L+++I +HGF+LWA+M
Sbjct: 10  SLLFLLL-----PLCSSQENTRSLAIDVNGPVETSPISEKLNPKLVYEIKVHGFMLWAAM 69

Query: 72  GFLMPVGILVIRM---------------------------------------------SN 131
           G LMP+GI+ IR+                                             ++
Sbjct: 70  GVLMPIGIISIRLMSIKDQPIITLRRLFFLHVTSQMVAVILVTIGAVMSVINFNNSFSNH 129

Query: 132 REQCG----------------RRLKGSRTRSLWFFIHWMLGTAVSLLGVFNVYSGLFAYH 164
            +Q G                R  +  + R  WF  HW+LGT++++LG+ N+Y+GL AY 
Sbjct: 130 HQQLGIGLYVIVWFQALLGFLRPPREEKARRKWFVGHWILGTSIAILGIINIYTGLHAYA 189

The following BLAST results are available for this feature:

BLAST of Carg14784 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037492.1	2.4e-101	100.00	Cytochrome b561 domain-containing protein, partial [Cucurbita argyrosperma subsp...	[more]
XP_022940301.1	3.5e-92	75.89	cytochrome b561 domain-containing protein At4g18260-like isoform X2 [Cucurbita m...	[more]
KAG6607978.1	1.0e-91	75.98	Cytochrome b561 domain-containing protein, partial [Cucurbita argyrosperma subsp...	[more]
XP_022940300.1	8.6e-91	75.59	cytochrome b561 domain-containing protein At4g18260-like isoform X1 [Cucurbita m...	[more]
XP_022981110.1	1.2e-89	74.31	cytochrome b561 domain-containing protein At4g18260-like isoform X2 [Cucurbita m...	[more]

BLAST of Carg14784 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q0WPS2	2.7e-23	32.47	Cytochrome b561 domain-containing protein At4g18260 OS=Arabidopsis thaliana OX=3...	[more]
O80854	4.6e-23	31.80	Cytochrome b561 domain-containing protein At2g30890 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Carg14784 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FI32	1.7e-92	75.89	cytochrome b561 domain-containing protein At4g18260-like isoform X2 OS=Cucurbita...	[more]
A0A6J1FQ60	4.1e-91	75.59	cytochrome b561 domain-containing protein At4g18260-like isoform X1 OS=Cucurbita...	[more]
A0A6J1IYJ3	6.0e-90	74.31	cytochrome b561 domain-containing protein At4g18260-like isoform X2 OS=Cucurbita...	[more]
A0A6J1J124	1.5e-88	74.02	cytochrome b561 domain-containing protein At4g18260-like isoform X1 OS=Cucurbita...	[more]
A0A6J1II03	9.6e-80	67.44	cytochrome b561 domain-containing protein At4g18260-like OS=Cucurbita maxima OX=...	[more]

BLAST of Carg14784 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G18260.1	1.9e-24	32.47	Cytochrome b561/ferric reductase transmembrane protein family	[more]
AT2G30890.1	3.3e-24	31.80	Cytochrome b561/ferric reductase transmembrane protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR045150	Transmembrane reductase CYB561D1/2	PANTHER	PTHR15422	OS05G0565100 PROTEIN	coord: 9..90 coord: 91..193
None	No IPR available	PANTHER	PTHR15422:SF42	EUKARYOTIC CYTOCHROME B561 PROTEIN	coord: 9..90 coord: 91..193
None	No IPR available	CDD	cd08760	Cyt_b561_FRRS1_like	coord: 37..155 e-value: 5.22798E-12 score: 59.6658

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg14784-RA	Carg14784-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0140575	transmembrane monodehydroascorbate reductase activity