Carg14782 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg14782 vs. NCBI nr
Match: KAG7037490.1 (hypothetical protein SDJN02_01117, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 338.2 bits (866), Expect = 4.2e-89
Identity = 173/173 (100.00%), Postives = 173/173 (100.00%), Query Frame = 0

Query: 1   MGPYFGAILEWDRSAAMADLVLIRPSGAPRAGSVRFNVALEALKSCAEIAMSLKNIGEKA 60
           MGPYFGAILEWDRSAAMADLVLIRPSGAPRAGSVRFNVALEALKSCAEIAMSLKNIGEKA
Sbjct: 1   MGPYFGAILEWDRSAAMADLVLIRPSGAPRAGSVRFNVALEALKSCAEIAMSLKNIGEKA 60

Query: 61  EFAWKRIKSMLHSGRETTENSNSLGEKTSTAAEKSNEGKENVTPTNGNGKVAAAAAKLFV 120
           EFAWKRIKSMLHSGRETTENSNSLGEKTSTAAEKSNEGKENVTPTNGNGKVAAAAAKLFV
Sbjct: 61  EFAWKRIKSMLHSGRETTENSNSLGEKTSTAAEKSNEGKENVTPTNGNGKVAAAAAKLFV 120

Query: 121 MSMGKHKFLEAHGLEQWWSDDLQHQLALHDSKQLKSLYTLASAQQPTPGRPSR 174
           MSMGKHKFLEAHGLEQWWSDDLQHQLALHDSKQLKSLYTLASAQQPTPGRPSR
Sbjct: 121 MSMGKHKFLEAHGLEQWWSDDLQHQLALHDSKQLKSLYTLASAQQPTPGRPSR 173

BLAST of Carg14782 vs. NCBI nr
Match: KAG6607976.1 (hypothetical protein SDJN03_01318, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 236.1 bits (601), Expect = 2.3e-58
Identity = 123/124 (99.19%), Postives = 123/124 (99.19%), Query Frame = 0

Query: 51  MSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGEKTSTAA-EKSNEGKENVTPTNGNG 110
           MSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGEKTSTAA EKSNEGKENVTPTNGNG
Sbjct: 1   MSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGEKTSTAAVEKSNEGKENVTPTNGNG 60

Query: 111 KVAAAAAKLFVMSMGKHKFLEAHGLEQWWSDDLQHQLALHDSKQLKSLYTLASAQQPTPG 170
           KVAAAAAKLFVMSMGKHKFLEAHGLEQWWSDDLQHQLALHDSKQLKSLYTLASAQQPTPG
Sbjct: 61  KVAAAAAKLFVMSMGKHKFLEAHGLEQWWSDDLQHQLALHDSKQLKSLYTLASAQQPTPG 120

Query: 171 RPSR 174
           RPSR
Sbjct: 121 RPSR 124

BLAST of Carg14782 vs. NCBI nr
Match: XP_038897095.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At4g10955 [Benincasa hispida])

HSP 1 Score: 199.9 bits (507), Expect = 1.8e-47
Identity = 141/296 (47.64%), Postives = 147/296 (49.66%), Query Frame = 0

Query: 2   GPYFGAILEWDRSAAMADLVLIRPSGAPRA------------------------------ 61
           G  FGAILEWDRSAAMADLVLIRPSGAPRA                              
Sbjct: 104 GSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESL 163

Query: 62  -GSVRFNVALEALKSCAE------------------------------------------ 121
            GSVRFNVALEALKS AE                                          
Sbjct: 164 KGSVRFNVALEALKSVAESYGSNNVCVAGHSLGAGFALQVGKALAKEGIYVETHLFNPPS 223

Query: 122 --IAMSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGEKT------------------ 174
             +AMSL+NIGEKAEFAWKRIKSML SG ETTENSN   EK                   
Sbjct: 224 VSVAMSLRNIGEKAEFAWKRIKSMLPSGNETTENSNIEEEKAYGGAGLKSWVSNLNRLKN 283

BLAST of Carg14782 vs. NCBI nr
Match: XP_022132245.1 (GDSL esterase/lipase At4g10955 [Momordica charantia])

HSP 1 Score: 193.0 bits (489), Expect = 2.2e-45
Identity = 139/295 (47.12%), Postives = 146/295 (49.49%), Query Frame = 0

Query: 2   GPYFGAILEWDRSAAMADLVLIRPSGAPRA------------------------------ 61
           G  FGAILEWDRSAAMADLVLIRPSGAPRA                              
Sbjct: 103 GSIFGAILEWDRSAAMADLVLIRPSGAPRAVLVLRGTLLKSPTIRRDIEDDLRFFAWESL 162

Query: 62  -GSVRFNVALEALKSCAE------------------------------------------ 121
            GSVRF VALEALKS AE                                          
Sbjct: 163 KGSVRFKVALEALKSVAESYGSNNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPS 222

Query: 122 --IAMSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGEKTSTAA-------------- 174
             +AMSL+NIGEKAEFAWKR+KSML SG ETTE SN  GEK   AA              
Sbjct: 223 VSVAMSLRNIGEKAEFAWKRLKSMLPSGSETTEASNIEGEKPPGAAGLKSWVSNLNRKNP 282

BLAST of Carg14782 vs. NCBI nr
Match: XP_022936957.1 (GDSL esterase/lipase At4g10955 isoform X2 [Cucurbita moschata])

HSP 1 Score: 191.0 bits (484), Expect = 8.3e-45
Identity = 138/296 (46.62%), Postives = 144/296 (48.65%), Query Frame = 0

Query: 2   GPYFGAILEWDRSAAMADLVLIRPSGAPRA------------------------------ 61
           G  FGAILEWDRSAAMADLVLIRPSGAPRA                              
Sbjct: 102 GSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESL 161

Query: 62  -GSVRFNVALEALKSCAE------------------------------------------ 121
            GSVRFNVAL+ALKS AE                                          
Sbjct: 162 KGSVRFNVALKALKSVAESYGSSNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPS 221

Query: 122 --IAMSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGEKT------------------ 174
             +AMSL+NIGEKAEFAWKRIKSML SG ETT NS+  GEK                   
Sbjct: 222 VSVAMSLRNIGEKAEFAWKRIKSMLPSGSETTGNSSMEGEKASGGGAGFKNWVANLNRLK 281

BLAST of Carg14782 vs. ExPASy TrEMBL
Match: A0A6J1BRX2 (GDSL esterase/lipase At4g10955 OS=Momordica charantia OX=3673 GN=LOC111005145 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 1.1e-45
Identity = 139/295 (47.12%), Postives = 146/295 (49.49%), Query Frame = 0

Query: 2   GPYFGAILEWDRSAAMADLVLIRPSGAPRA------------------------------ 61
           G  FGAILEWDRSAAMADLVLIRPSGAPRA                              
Sbjct: 103 GSIFGAILEWDRSAAMADLVLIRPSGAPRAVLVLRGTLLKSPTIRRDIEDDLRFFAWESL 162

Query: 62  -GSVRFNVALEALKSCAE------------------------------------------ 121
            GSVRF VALEALKS AE                                          
Sbjct: 163 KGSVRFKVALEALKSVAESYGSNNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPS 222

Query: 122 --IAMSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGEKTSTAA-------------- 174
             +AMSL+NIGEKAEFAWKR+KSML SG ETTE SN  GEK   AA              
Sbjct: 223 VSVAMSLRNIGEKAEFAWKRLKSMLPSGSETTEASNIEGEKPPGAAGLKSWVSNLNRKNP 282

BLAST of Carg14782 vs. ExPASy TrEMBL
Match: A0A6J1F9S8 (GDSL esterase/lipase At4g10955 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443389 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 4.0e-45
Identity = 138/296 (46.62%), Postives = 144/296 (48.65%), Query Frame = 0

Query: 2   GPYFGAILEWDRSAAMADLVLIRPSGAPRA------------------------------ 61
           G  FGAILEWDRSAAMADLVLIRPSGAPRA                              
Sbjct: 102 GSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESL 161

Query: 62  -GSVRFNVALEALKSCAE------------------------------------------ 121
            GSVRFNVAL+ALKS AE                                          
Sbjct: 162 KGSVRFNVALKALKSVAESYGSSNVCIAGHSLGAGFALQVGKALAKEGIYVETHLFNPPS 221

Query: 122 --IAMSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGEKT------------------ 174
             +AMSL+NIGEKAEFAWKRIKSML SG ETT NS+  GEK                   
Sbjct: 222 VSVAMSLRNIGEKAEFAWKRIKSMLPSGSETTGNSSMEGEKASGGGAGFKNWVANLNRLK 281

BLAST of Carg14782 vs. ExPASy TrEMBL
Match: A0A5D3CGI8 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold504G00550 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 9.0e-45
Identity = 134/291 (46.05%), Postives = 144/291 (49.48%), Query Frame = 0

Query: 2   GPYFGAILEWDRSAAMADLVLIRPSGAPRA------------------------------ 61
           G  FGAILEWDRSAAMADLVLIRPSGAPRA                              
Sbjct: 106 GSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESL 165

Query: 62  -GSVRFNVALEALKSCAE------------------------------------------ 121
            GSVRFNVALEALKS AE                                          
Sbjct: 166 KGSVRFNVALEALKSVAESYGSNNVCVAGHSLGAGFALQVGKALAKEGIYVETHLFNPPS 225

Query: 122 --IAMSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGE-------------------- 168
             +AMSL+NIGEKAEFAWKRIKSM+ SG ETTENS+++ E                    
Sbjct: 226 VSVAMSLRNIGEKAEFAWKRIKSMIPSGSETTENSSNIEEEKSYGVTGLKSWVSNLNRLK 285

BLAST of Carg14782 vs. ExPASy TrEMBL
Match: A0A1S4E575 (GDSL esterase/lipase At4g10955 isoform X4 OS=Cucumis melo OX=3656 GN=LOC103503049 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 9.0e-45
Identity = 134/291 (46.05%), Postives = 144/291 (49.48%), Query Frame = 0

Query: 2   GPYFGAILEWDRSAAMADLVLIRPSGAPRA------------------------------ 61
           G  FGAILEWDRSAAMADLVLIRPSGAPRA                              
Sbjct: 48  GSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESL 107

Query: 62  -GSVRFNVALEALKSCAE------------------------------------------ 121
            GSVRFNVALEALKS AE                                          
Sbjct: 108 KGSVRFNVALEALKSVAESYGSNNVCVAGHSLGAGFALQVGKALAKEGIYVETHLFNPPS 167

Query: 122 --IAMSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGE-------------------- 168
             +AMSL+NIGEKAEFAWKRIKSM+ SG ETTENS+++ E                    
Sbjct: 168 VSVAMSLRNIGEKAEFAWKRIKSMIPSGSETTENSSNIEEEKSYGVTGLKSWVSNLNRLK 227

BLAST of Carg14782 vs. ExPASy TrEMBL
Match: A0A1S3CNX1 (GDSL esterase/lipase At4g10955 isoform X3 OS=Cucumis melo OX=3656 GN=LOC103503049 PE=4 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 9.0e-45
Identity = 134/291 (46.05%), Postives = 144/291 (49.48%), Query Frame = 0

Query: 2   GPYFGAILEWDRSAAMADLVLIRPSGAPRA------------------------------ 61
           G  FGAILEWDRSAAMADLVLIRPSGAPRA                              
Sbjct: 85  GSIFGAILEWDRSAAMADLVLIRPSGAPRAVLALRGTLLKSPTIRRDIEDDLRFLAWESL 144

Query: 62  -GSVRFNVALEALKSCAE------------------------------------------ 121
            GSVRFNVALEALKS AE                                          
Sbjct: 145 KGSVRFNVALEALKSVAESYGSNNVCVAGHSLGAGFALQVGKALAKEGIYVETHLFNPPS 204

Query: 122 --IAMSLKNIGEKAEFAWKRIKSMLHSGRETTENSNSLGE-------------------- 168
             +AMSL+NIGEKAEFAWKRIKSM+ SG ETTENS+++ E                    
Sbjct: 205 VSVAMSLRNIGEKAEFAWKRIKSMIPSGSETTENSSNIEEEKSYGVTGLKSWVSNLNRLK 264

BLAST of Carg14782 vs. TAIR 10
Match: AT5G50890.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 2.1e-25
Identity = 100/288 (34.72%), Postives = 121/288 (42.01%), Query Frame = 0

Query: 2   GPYFGAILEWDRSAAMADLVLIRPSGAPRA------------------------------ 61
           G  FGA+LEWDR+AAMADLV+IRPSGAP+A                              
Sbjct: 123 GSIFGAVLEWDRAAAMADLVVIRPSGAPKAVLALRGTILKSLTMRRDIEDDLRFLAWESL 182

Query: 62  -GSVRFNVALEALKSCA------------------------------------------- 121
            GSVRFNVALEAL+S A                                           
Sbjct: 183 KGSVRFNVALEALQSVATRYGSSNVCVVGHSLGAGFALQVGKSLAKEGLFVDAHLFNPPS 242

Query: 122 -EIAMSLKNIGEKAEFAWKRIKSMLHSGRE-TTENSNS---------------------- 161
             +AMSL+NI EKA  AWKR+ SML    E   +NSN                       
Sbjct: 243 ISVAMSLRNISEKAGVAWKRLMSMLPPKHEPLIQNSNEGQVSPSNVSSGFRNWVPSFYGS 302