Carg14454 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg14454
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionFAD synthase
LocationCarg_Chr18: 8517233 .. 8520435 (+)
RNA-Seq ExpressionCarg14454
SyntenyCarg14454
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAAGAACCAAGAAGCTACGAATTCAACCAATTCAAAGGAGCGAGGACAAGTGTCGTCTACTCGGGTTCGCTACGCCCTTTCGACAGGTGATATGAAATACGTCTCCGAGCTTTTAGGCCGTAGACATCGTCTAGTTTTGATGGCGAAAGACGTAGAGGGATTAAGTTATAGCAATAGTAGAGTGTCAGTTCCAAGATCATGTTCATTAAACTTAGCACCAAAAGAAGGTTTATATGATAAGTGCTTTGTCTGGTCGTTTGATCAGAACTTGATTCCATGTCGGGTCGTTATTGACTCGAGTCATGTTCATATCGAGTTAGATGAGTTAGCTACGTGTCGTCATCCTGTAGGAACCGATGACTACATAAGTGTTGAGTTTGACGATGAAGGTGTATGATTATATTCGATTCACCTCCGCTTACTTGTTCCTATACGTACATCGGTGAGATCCATCGTTGTTTTTCCTAACAGAGGTATGTTGCTTTATCTGATGATTCAAATTCAGGGTGTTTTAGCAAATTATGTTCTAAGTAACTCTGAGCCCGTTAATAGATATTGTTCTTTTTGGGCTTTCCCATTCGGTTTCCCCTCAAGGTTTTTAAAACGTGTTTGCTAGAGAGAGATTTCCATGCCCTTATAAAAAATGTTTTGTTCTTCTCTCCAACCGAGCGGAGATTTCACAATCCACCCCCCTTGGAGGCCTAGCGTCCTCGCTGATACTCATTCCTCTCTCTAATCGGTGTAGGATCTCACAATCCACCCTCTTTTGTGGCTCAACATTCTCGCTGGCACTCCACCCGGTGTTCACCCCATTCAGTGTGCCAGCAAGAACGCTAGGTGTTACAGTCATGCTTGAAAGGAGATTGTGACACTAGGGGTTCCGAACAGGGATGAATTGTGAGATCCCACATCGATTTGTGAGGGAAAAGGTGCCAGCGAGAATGCGGGGCTCTGAAGGGGGATGGATTTTGAGATCCCACATCCATTGGGGAGGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGTCGCGTTTTAAAACCTTGAGGAGAACCCAAAAGGGAAAGCTCAAATCAGACAGAGGTTTGTTACAAATGATATCAAAGCCAAACATTAGACAGTGTACGAGCGAGGACGCTGGGCCCTGAATGAGGGTAAATTGTGAGACCCCGCATCTATTGGAGAGAGGAACGAGTGCCAACAACGACGCTGGGCTCCAAAGGAGGGTGAATTGTGAGATCCCACATTAATTAGAGAGCGGAACGAGTGCCAACGAGGACTCTCGACCCTGAAAGGGGGTAGACTCTAAGATCCCACATCCGTCAGGGAGGAAAACAAAACATCCTTCCTAATAGACGCGTTTTAAAACCTTGAGGGGAAACCCGAAAGGGAAAGCCCAAACATGACAATATCTATTAGCCGTGGACTTGAGTTCTTACATTAAAGATCTTTCATACGTCGAAAAGAAAAGACGTCTGTACCATTCGAGACCTTTATTTCAATCCTTAGAAAGCCCGTTCTCGAGGAACTTAAAAGCCATCTTATAACTCAAAAACCCCATAAACCAGAACTCGAAACCCATCAACAGTCACCACTTGAATGTACTTTTTTGATGGATTGTTTACATTTTCACTTTGGTTAACTCTCTTCATCTTCTTCATTGGGATCATAACCTATAAACATTGCAGGAAACATGTCAGAACCCACACAAACAGACAGACAGGGTGACAGAAAGTCAAAACATACTTTGTAATTCATTCTGGTCTGTTCTTCATGTAGAGAAGAGAATATGATTGGTCTGTCACTGCAGAAGGCGACCACATGGGTGGAGACAAAGAGGAGGCCTGCTATTGGACCTGCTGTTGTTGACAAATAACAGTGTGAAGAGCTCAGCAACTTGTCATCTTGTCTGAGTTTGAACATCTTCTTAAATATCTTCTCACGTCTACCAATCTGAACAAGCTTTGCTCCCAACCATAGCTTCCGTTTCACGGTTTTGGTGACGCTCGGTGATAGTTTAACTGCACGGCGTCGTTCCAATGGAACTGTCAAGCAATTTGTTCCAGGGGAATATGTTCGTTTGTATGATTATAAAAAGTTTAACGAGTTGAGTTATGTTCAGGTTGACCTACAAAAGTTGTCGTAGTGGATTTTGTGATTATGCGGTACTCACTTCCTAATACTAAGTGAGTTAAGTTGATTTGTATTTGTGTTGCCTACGGCTGGGTAATGTATGCTCAAGCATTTTGCCTTGTTTTGAGAATAAGGTTGTAGAAAACGGGGACAGAGAGATTTTGTGATGTCCCACATTAGTTAGGGAAGAGAACAAAACACCCTTTATAAGGGTGTGAAAACCTTCTCCTAGCGTATGCGTTTTAAAGCCTTGATGGGAAGCCCGAAGAGGACAATATCTGCTAGCAGTGGATCTGGGTCGTTACAAATGGTATCAGAGCCAGACACTGGACGATGTGCCAGTCTTCTCGCTGTTCCTCGAAGGGGGATAGACACGAGGCGGTGTGCCCGTAAGGATGCTGGGCCCCAAAGGAGGGTGGATTCTGAGGGGGTCCCACATCGATTGGAGAAAGGAACGAGTGCTAGCGAGGACGCTGGGCCCCGAAAGGGGGTGGATTGTGATGTCCCACATTGGTTGAGGAGGAGAACAAAATACCCTTTATAAGGGTGTGGAAACCTTCCCCTGACAGACGTGTTTTAAAGCCTGGAGGGGAAGCCCGAAAAGGAAAGCCTAAAGAGGACAATATTTGCTAGCGGTGGATCTGGATTGTTACAGATTTTGAGAGAGAGATTGAAAGCAATGGACTGAAAGCAATGTTAAGTTAGAATTGCCTAAAAAAAATGGATGTGATTGCACCTAAAAAAGTCATTTCGGTTCGTTATCGGTTAGGTATCAGTGGGAGCATCAACATCAAAGAGTTAACTAAGTATCAGATCGGAAGTGAAAGTTGGACGTATTACATAATCCTAATTGGACTTGTAAGTAGGTTTATGGAGACACCAAGAGCCCCACATTTGAAGGAAGCAAAGAGGTCGACCGGGCGGGAGAAAAACCCCCTGAAACCGGACAAAACCCATCTAAGATCCTATGTGCTAGCTTAGTAGGCCCAAAACCTAAAGCTAACATTTCAATCAAAAATCGACGTCGGGTCGCGACGAGACGAAAAAGGCAATGA

mRNA sequence

ATGGATAAGAACCAAGAAGCTACGAATTCAACCAATTCAAAGGAGCGAGGACAAGTGTCGTCTACTCGGGTTCGCTACGCCCTTTCGACAGGTGATATGAAATACGTCTCCGAGCTTTTAGGCCGTAGACATCGTCTAGTTTTGATGGCGAAAGACGTAGAGGGATTAAGTTATAGCAATAGTAGAGTGTCAGTTCCAAGATCATGTTCATTAAACTTAGCACCAAAAGAAGGTTTATATGATAAGTGCTTTGTCTGGTCGTTTGATCAGAACTTGATTCCATGTCGGGTCGTTATTGACTCGAGTCATGTTCATATCGAGTTAGATGAGTTAGCTACGTGTCGTCATCCTGTAGGAACCGATGACTACATAAGTGTTGAGTTTGACGATGAAGGTGTTTATGGAGACACCAAGAGCCCCACATTTGAAGGAAGCAAAGAGGTCGACCGGGCGGGAGAAAAACCCCCTGAAACCGGACAAAACCCATCTAAGATCCTATGTGCTAGCTTAGTAGGCCCAAAACCTAAAGCTAACATTTCAATCAAAAATCGACGTCGGGTCGCGACGAGACGAAAAAGGCAATGA

Coding sequence (CDS)

ATGGATAAGAACCAAGAAGCTACGAATTCAACCAATTCAAAGGAGCGAGGACAAGTGTCGTCTACTCGGGTTCGCTACGCCCTTTCGACAGGTGATATGAAATACGTCTCCGAGCTTTTAGGCCGTAGACATCGTCTAGTTTTGATGGCGAAAGACGTAGAGGGATTAAGTTATAGCAATAGTAGAGTGTCAGTTCCAAGATCATGTTCATTAAACTTAGCACCAAAAGAAGGTTTATATGATAAGTGCTTTGTCTGGTCGTTTGATCAGAACTTGATTCCATGTCGGGTCGTTATTGACTCGAGTCATGTTCATATCGAGTTAGATGAGTTAGCTACGTGTCGTCATCCTGTAGGAACCGATGACTACATAAGTGTTGAGTTTGACGATGAAGGTGTTTATGGAGACACCAAGAGCCCCACATTTGAAGGAAGCAAAGAGGTCGACCGGGCGGGAGAAAAACCCCCTGAAACCGGACAAAACCCATCTAAGATCCTATGTGCTAGCTTAGTAGGCCCAAAACCTAAAGCTAACATTTCAATCAAAAATCGACGTCGGGTCGCGACGAGACGAAAAAGGCAATGA

Protein sequence

MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGTDDYISVEFDDEGVYGDTKSPTFEGSKEVDRAGEKPPETGQNPSKILCASLVGPKPKANISIKNRRRVATRRKRQ
Homology
BLAST of Carg14454 vs. NCBI nr
Match: KAG7012829.1 (FAD synthetase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 384.4 bits (986), Expect = 5.7e-103
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN
Sbjct: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
           SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT
Sbjct: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120

Query: 121 DDYISVEFDDEGVYGDTKSPTFEGSKEVDRAGEKPPETGQNPSKILCASLVGPKPKANIS 180
           DDYISVEFDDEGVYGDTKSPTFEGSKEVDRAGEKPPETGQNPSKILCASLVGPKPKANIS
Sbjct: 121 DDYISVEFDDEGVYGDTKSPTFEGSKEVDRAGEKPPETGQNPSKILCASLVGPKPKANIS 180

Query: 181 IKNRRRVATRRKRQ 195
           IKNRRRVATRRKRQ
Sbjct: 181 IKNRRRVATRRKRQ 194

BLAST of Carg14454 vs. NCBI nr
Match: KAG6573753.1 (FAD synthetase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 268.9 bits (686), Expect = 3.5e-68
Identity = 133/133 (100.00%), Postives = 133/133 (100.00%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN
Sbjct: 234 MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 293

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
           SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT
Sbjct: 294 SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 353

Query: 121 DDYISVEFDDEGV 134
           DDYISVEFDDEGV
Sbjct: 354 DDYISVEFDDEGV 366

BLAST of Carg14454 vs. NCBI nr
Match: XP_022945276.1 (FAD synthetase 2, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 254.2 bits (648), Expect = 9.0e-64
Identity = 126/133 (94.74%), Postives = 128/133 (96.24%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           MDKNQE T+STNSKE+GQV STRVRY LSTGDMKYVSELLG RHRLVLMAKDVEGLSYSN
Sbjct: 186 MDKNQEFTDSTNSKEQGQVLSTRVRYTLSTGDMKYVSELLGCRHRLVLMAKDVEGLSYSN 245

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
           SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNL PCRVVIDSSHVHIELDELATCRHPVGT
Sbjct: 246 SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLTPCRVVIDSSHVHIELDELATCRHPVGT 305

Query: 121 DDYISVEFDDEGV 134
           DDYISVEFDDEGV
Sbjct: 306 DDYISVEFDDEGV 318

BLAST of Carg14454 vs. NCBI nr
Match: XP_022945275.1 (FAD synthetase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 254.2 bits (648), Expect = 9.0e-64
Identity = 126/133 (94.74%), Postives = 128/133 (96.24%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           MDKNQE T+STNSKE+GQV STRVRY LSTGDMKYVSELLG RHRLVLMAKDVEGLSYSN
Sbjct: 227 MDKNQEFTDSTNSKEQGQVLSTRVRYTLSTGDMKYVSELLGCRHRLVLMAKDVEGLSYSN 286

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
           SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNL PCRVVIDSSHVHIELDELATCRHPVGT
Sbjct: 287 SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLTPCRVVIDSSHVHIELDELATCRHPVGT 346

Query: 121 DDYISVEFDDEGV 134
           DDYISVEFDDEGV
Sbjct: 347 DDYISVEFDDEGV 359

BLAST of Carg14454 vs. NCBI nr
Match: XP_022945277.1 (FAD synthetase 2, chloroplastic-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 254.2 bits (648), Expect = 9.0e-64
Identity = 126/133 (94.74%), Postives = 128/133 (96.24%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           MDKNQE T+STNSKE+GQV STRVRY LSTGDMKYVSELLG RHRLVLMAKDVEGLSYSN
Sbjct: 161 MDKNQEFTDSTNSKEQGQVLSTRVRYTLSTGDMKYVSELLGCRHRLVLMAKDVEGLSYSN 220

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
           SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNL PCRVVIDSSHVHIELDELATCRHPVGT
Sbjct: 221 SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLTPCRVVIDSSHVHIELDELATCRHPVGT 280

Query: 121 DDYISVEFDDEGV 134
           DDYISVEFDDEGV
Sbjct: 281 DDYISVEFDDEGV 293

BLAST of Carg14454 vs. ExPASy Swiss-Prot
Match: Q8VZR0 (FAD synthetase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF2 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.0e-22
Identity = 59/129 (45.74%), Postives = 83/129 (64.34%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           M +     +S +SK+RGQVSSTRVR AL+ GDM+YVSELLGR HRL+L  +  +    S 
Sbjct: 240 MKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQD--MPSE 299

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
             +SVPRS  LNL P  G+Y  C +   D++ +PC VV+D+S++H+E +E+  C      
Sbjct: 300 RMISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEEVRLCNLDWSQ 359

Query: 121 D-DYISVEF 129
           +   +SVEF
Sbjct: 360 EFRLVSVEF 366

BLAST of Carg14454 vs. ExPASy Swiss-Prot
Match: Q10BX9 (FAD synthetase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBF PE=3 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 3.7e-20
Identity = 55/122 (45.08%), Postives = 81/122 (66.39%), Query Frame = 0

Query: 8   TNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSNSRVSVPR 67
           T S NS ++GQVSS+RVR+AL+ GDM+YVSELLGR+HRLVL  K  E       R+ +P+
Sbjct: 272 TTSVNSSDKGQVSSSRVRHALAMGDMEYVSELLGRKHRLVLTVK--ENHLQERKRIMLPK 331

Query: 68  SCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIEL-DELATCRHPVGTDDYISV 127
           SC LN+ P +GLY+ C + +   +L  CRV+I+S  + IE+ DE +   + +  +  + +
Sbjct: 332 SCMLNMPPADGLYENCDLLN-GGHLGLCRVIINSETIVIEMKDENSLLPNTIQENQQLGI 390

Query: 128 EF 129
           EF
Sbjct: 392 EF 390

BLAST of Carg14454 vs. ExPASy Swiss-Prot
Match: Q9FMW8 (FAD synthetase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF1 PE=1 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.4e-19
Identity = 52/114 (45.61%), Postives = 79/114 (69.30%), Query Frame = 0

Query: 1   MDKNQEATNS----TNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGL 60
           MDKNQ + N+    + SKERGQVSSTRVR+AL+ GD++YV+ELLGR HR++   +  + L
Sbjct: 222 MDKNQVSVNTEEEDSKSKERGQVSSTRVRHALAAGDVRYVTELLGRPHRVISRTR-TQDL 281

Query: 61  SYSNSRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDE 111
           +    R+S+  S  LNL P  G+Y  C +   D++ I C+V++D+S+++IE +E
Sbjct: 282 TSKRGRISLQTSSLLNLPPGNGVYKACSLIVGDKHPISCKVIVDTSNLYIETEE 334

BLAST of Carg14454 vs. ExPASy TrEMBL
Match: A0A6J1G0H2 (FAD synthetase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449571 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.3e-64
Identity = 126/133 (94.74%), Postives = 128/133 (96.24%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           MDKNQE T+STNSKE+GQV STRVRY LSTGDMKYVSELLG RHRLVLMAKDVEGLSYSN
Sbjct: 227 MDKNQEFTDSTNSKEQGQVLSTRVRYTLSTGDMKYVSELLGCRHRLVLMAKDVEGLSYSN 286

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
           SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNL PCRVVIDSSHVHIELDELATCRHPVGT
Sbjct: 287 SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLTPCRVVIDSSHVHIELDELATCRHPVGT 346

Query: 121 DDYISVEFDDEGV 134
           DDYISVEFDDEGV
Sbjct: 347 DDYISVEFDDEGV 359

BLAST of Carg14454 vs. ExPASy TrEMBL
Match: A0A6J1G0E4 (FAD synthetase 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449571 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.3e-64
Identity = 126/133 (94.74%), Postives = 128/133 (96.24%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           MDKNQE T+STNSKE+GQV STRVRY LSTGDMKYVSELLG RHRLVLMAKDVEGLSYSN
Sbjct: 186 MDKNQEFTDSTNSKEQGQVLSTRVRYTLSTGDMKYVSELLGCRHRLVLMAKDVEGLSYSN 245

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
           SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNL PCRVVIDSSHVHIELDELATCRHPVGT
Sbjct: 246 SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLTPCRVVIDSSHVHIELDELATCRHPVGT 305

Query: 121 DDYISVEFDDEGV 134
           DDYISVEFDDEGV
Sbjct: 306 DDYISVEFDDEGV 318

BLAST of Carg14454 vs. ExPASy TrEMBL
Match: A0A6J1G0H1 (FAD synthetase 2, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111449571 PE=4 SV=1)

HSP 1 Score: 254.2 bits (648), Expect = 4.3e-64
Identity = 126/133 (94.74%), Postives = 128/133 (96.24%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           MDKNQE T+STNSKE+GQV STRVRY LSTGDMKYVSELLG RHRLVLMAKDVEGLSYSN
Sbjct: 161 MDKNQEFTDSTNSKEQGQVLSTRVRYTLSTGDMKYVSELLGCRHRLVLMAKDVEGLSYSN 220

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
           SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNL PCRVVIDSSHVHIELDELATCRHPVGT
Sbjct: 221 SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLTPCRVVIDSSHVHIELDELATCRHPVGT 280

Query: 121 DDYISVEFDDEGV 134
           DDYISVEFDDEGV
Sbjct: 281 DDYISVEFDDEGV 293

BLAST of Carg14454 vs. ExPASy TrEMBL
Match: A0A6J1KGG1 (FAD synthase OS=Cucurbita maxima OX=3661 GN=LOC111493664 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 4.4e-48
Identity = 102/134 (76.12%), Postives = 117/134 (87.31%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGL--SY 60
           MD+NQ+  NSTNS ERGQVSSTRVRYALS GDMKYVSELLGRRHRL+LMAK +EGL  S 
Sbjct: 232 MDRNQKVVNSTNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGLSNSN 291

Query: 61  SNSRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPV 120
           SNSR+S P+SC LNLAPKEGLY+KCFV + D +LIPCRVVIDS+HVHIE+D++ T RHPV
Sbjct: 292 SNSRLSAPKSCLLNLAPKEGLYNKCFVCTSDDDLIPCRVVIDSTHVHIEMDDIGT-RHPV 351

Query: 121 GTDDYISVEFDDEG 133
           GT +Y+SVEFD EG
Sbjct: 352 GTQNYLSVEFDHEG 364

BLAST of Carg14454 vs. ExPASy TrEMBL
Match: A0A6J1GWB8 (FAD synthase OS=Cucurbita moschata OX=3662 GN=LOC111458123 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 9.7e-48
Identity = 101/132 (76.52%), Postives = 116/132 (87.88%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           MD+NQ+  NSTNS ERGQVSSTRVRYALS GDMKYVSELLGRRHRL+LMAK +EGL  SN
Sbjct: 232 MDRNQKVVNSTNSMERGQVSSTRVRYALSIGDMKYVSELLGRRHRLILMAKGLEGL--SN 291

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
           SRVS P+SC LNLAPKEGLY+ CFV + D++LIPCRVVIDS+HVHIE+D++ T RHPVGT
Sbjct: 292 SRVSAPKSCLLNLAPKEGLYNTCFVCTSDEDLIPCRVVIDSTHVHIEMDDIGT-RHPVGT 351

Query: 121 DDYISVEFDDEG 133
            +Y+SVEFD EG
Sbjct: 352 QNYLSVEFDHEG 360

BLAST of Carg14454 vs. TAIR 10
Match: AT5G08340.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 7.4e-24
Identity = 59/129 (45.74%), Postives = 83/129 (64.34%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           M +     +S +SK+RGQVSSTRVR AL+ GDM+YVSELLGR HRL+L  +  +    S 
Sbjct: 240 MKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQD--MPSE 299

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDELATCRHPVGT 120
             +SVPRS  LNL P  G+Y  C +   D++ +PC VV+D+S++H+E +E+  C      
Sbjct: 300 RMISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEEVRLCNLDWSQ 359

Query: 121 D-DYISVEF 129
           +   +SVEF
Sbjct: 360 EFRLVSVEF 366

BLAST of Carg14454 vs. TAIR 10
Match: AT5G08340.2 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 104.4 bits (259), Expect = 1.1e-22
Identity = 54/110 (49.09%), Postives = 75/110 (68.18%), Query Frame = 0

Query: 1   MDKNQEATNSTNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGLSYSN 60
           M +     +S +SK+RGQVSSTRVR AL+ GDM+YVSELLGR HRL+L  +  +    S 
Sbjct: 240 MKQGSAKRDSGDSKDRGQVSSTRVRQALAAGDMRYVSELLGRAHRLILRVRTQD--MPSE 299

Query: 61  SRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDE 111
             +SVPRS  LNL P  G+Y  C +   D++ +PC VV+D+S++H+E +E
Sbjct: 300 RMISVPRSSILNLPPGIGIYKACLLLVGDESSVPCTVVVDTSNIHVETEE 347

BLAST of Carg14454 vs. TAIR 10
Match: AT5G23330.1 (Nucleotidylyl transferase superfamily protein )

HSP 1 Score: 97.8 bits (242), Expect = 1.0e-20
Identity = 52/114 (45.61%), Postives = 79/114 (69.30%), Query Frame = 0

Query: 1   MDKNQEATNS----TNSKERGQVSSTRVRYALSTGDMKYVSELLGRRHRLVLMAKDVEGL 60
           MDKNQ + N+    + SKERGQVSSTRVR+AL+ GD++YV+ELLGR HR++   +  + L
Sbjct: 222 MDKNQVSVNTEEEDSKSKERGQVSSTRVRHALAAGDVRYVTELLGRPHRVISRTR-TQDL 281

Query: 61  SYSNSRVSVPRSCSLNLAPKEGLYDKCFVWSFDQNLIPCRVVIDSSHVHIELDE 111
           +    R+S+  S  LNL P  G+Y  C +   D++ I C+V++D+S+++IE +E
Sbjct: 282 TSKRGRISLQTSSLLNLPPGNGVYKACSLIVGDKHPISCKVIVDTSNLYIETEE 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7012829.15.7e-103100.00FAD synthetase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosp... [more]
KAG6573753.13.5e-68100.00FAD synthetase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022945276.19.0e-6494.74FAD synthetase 2, chloroplastic-like isoform X2 [Cucurbita moschata][more]
XP_022945275.19.0e-6494.74FAD synthetase 2, chloroplastic-like isoform X1 [Cucurbita moschata][more]
XP_022945277.19.0e-6494.74FAD synthetase 2, chloroplastic-like isoform X3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q8VZR01.0e-2245.74FAD synthetase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF2 PE=1 SV... [more]
Q10BX93.7e-2045.08FAD synthetase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=RIBF P... [more]
Q9FMW81.4e-1945.61FAD synthetase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RIBF1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1G0H24.3e-6494.74FAD synthetase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1G0E44.3e-6494.74FAD synthetase 2, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1G0H14.3e-6494.74FAD synthetase 2, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1KGG14.4e-4876.12FAD synthase OS=Cucurbita maxima OX=3661 GN=LOC111493664 PE=4 SV=1[more]
A0A6J1GWB89.7e-4876.52FAD synthase OS=Cucurbita moschata OX=3662 GN=LOC111458123 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G08340.17.4e-2445.74Nucleotidylyl transferase superfamily protein [more]
AT5G08340.21.1e-2249.09Nucleotidylyl transferase superfamily protein [more]
AT5G23330.11.0e-2045.61Nucleotidylyl transferase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 134..166
NoneNo IPR availablePANTHERPTHR12714:SF20FAD SYNTHETASE 1, CHLOROPLASTIC-RELATEDcoord: 1..129
NoneNo IPR availablePANTHERPTHR12714PROTEIN-S ISOPRENYLCYSTEINE O-METHYLTRANSFERASEcoord: 1..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg14454-RACarg14454-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006747 FAD biosynthetic process
biological_process GO:0009231 riboflavin biosynthetic process
cellular_component GO:0009507 chloroplast
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0003919 FMN adenylyltransferase activity