Carg13997 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13997
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description50S ribosomal protein L23, chloroplastic
LocationCarg_Chr02: 6665638 .. 6665901 (+)
RNA-Seq ExpressionCarg13997
SyntenyCarg13997
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAGGAGGAATGTCTGGTTTGTCAACAAAACAAGTCTGGGACTCAATCTCGGAATCAAACATGTAGGATTTACAGACAAAACTACTTGGTTATTGGGAAAACTTCAATATACTTCTAATGTTGAATCGTGATCAATTAGGACAGAAATAAAGCATTGTGTTAAACTCTTCTTTGGTGTCAAGGTAATAGCTATGAATAGTCATCGACTTCCGAAAAAGAGTAGGAGAATGAGACCTGTTATGAGACATACAATGCATTAG

mRNA sequence

ATGGAGAGGAGGAATGTCTGGTTTGTCAACAAAACAAGTCTGGGACTCAATCTCGGAATCAAACATGTAGGATTTACAGACAAAACTACTTGGTTATTGGGAAAACTTCAATATACTTCTAATGTTGAATCGACAGAAATAAAGCATTGTGTTAAACTCTTCTTTGGTGTCAAGGTAATAGCTATGAATAGTCATCGACTTCCGAAAAAGAGTAGGAGAATGAGACCTGTTATGAGACATACAATGCATTAG

Coding sequence (CDS)

ATGGAGAGGAGGAATGTCTGGTTTGTCAACAAAACAAGTCTGGGACTCAATCTCGGAATCAAACATGTAGGATTTACAGACAAAACTACTTGGTTATTGGGAAAACTTCAATATACTTCTAATGTTGAATCGACAGAAATAAAGCATTGTGTTAAACTCTTCTTTGGTGTCAAGGTAATAGCTATGAATAGTCATCGACTTCCGAAAAAGAGTAGGAGAATGAGACCTGTTATGAGACATACAATGCATTAG

Protein sequence

MERRNVWFVNKTSLGLNLGIKHVGFTDKTTWLLGKLQYTSNVESTEIKHCVKLFFGVKVIAMNSHRLPKKSRRMRPVMRHTMH
Homology
[Close]
BLAST of Carg13997 vs. NCBI nr
Match: KAG7035768.1 (50S ribosomal protein L23, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 173.7 bits (439), Expect = 6.6e-40
Identity = 83/83 (100.00%), Postives = 83/83 (100.00%), Query Frame = 0 

Query: 1  MERRNVWFVNKTSLGLNLGIKHVGFTDKTTWLLGKLQYTSNVESTEIKHCVKLFFGVKVI 60
          MERRNVWFVNKTSLGLNLGIKHVGFTDKTTWLLGKLQYTSNVESTEIKHCVKLFFGVKVI
Sbjct: 1  MERRNVWFVNKTSLGLNLGIKHVGFTDKTTWLLGKLQYTSNVESTEIKHCVKLFFGVKVI 60

Query: 61 AMNSHRLPKKSRRMRPVMRHTMH 84
          AMNSHRLPKKSRRMRPVMRHTMH
Sbjct: 61 AMNSHRLPKKSRRMRPVMRHTMH 83

[Close]
BLAST of Carg13997 vs. NCBI nr
Match: YP_009731796.1 (ribosomal protein L23 [Siphonostegia chinensis] >YP_009731816.1 ribosomal protein L23 [Siphonostegia chinensis] >QHR84990.1 ribosomal protein L23 [Siphonostegia chinensis] >QHR85010.1 ribosomal protein L23 [Siphonostegia chinensis])

HSP 1 Score: 99.4 bits (246), Expect = 1.6e-17
Identity = 51/69 (73.91%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+ WLLGK QYTSNVES    TE+KH V+LFFGVKVIAMNSHRLP K RRM
Sbjct: 3  GIKNPVFTDKSIWLLGKNQYTSNVESGSTRTELKHWVELFFGVKVIAMNSHRLPGKGRRM 62

Query: 79 RPVMRHTMH 84
           P+M HTMH
Sbjct: 63 GPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. NCBI nr
Match: YP_009756322.1 (ribosomal protein L23 [Withania coagulans] >YP_009756343.1 ribosomal protein L23 [Withania coagulans] >QFR99349.1 ribosomal protein L23 [Withania somnifera] >DAC81886.1 TPA_asm: ribosomal protein L23 [Withania adpressa] >DAC81970.1 TPA_asm: ribosomal protein L23 [Withania riebeckii] >QFR99368.1 ribosomal protein L23 [Withania somnifera] >QJA26325.1 ribosomal protein L23 [Withania coagulans])

HSP 1 Score: 99.0 bits (245), Expect = 2.1e-17
Identity = 53/69 (76.81%), Postives = 57/69 (82.61%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+V FTDK+  LLGK QYTSNVES    TEIKH V+LFFGVKVIAMNSHRLP KSRRM
Sbjct: 3  GIKYVVFTDKSIRLLGKNQYTSNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKSRRM 62

Query: 79 RPVMRHTMH 84
           P+M HTMH
Sbjct: 63 GPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. NCBI nr
Match: YP_009671421.1 (ribosomal protein L23 [Passiflora obovata] >YP_009671437.1 ribosomal protein L23 [Passiflora obovata] >QCX30696.1 ribosomal protein L23 [Passiflora obovata] >QCX30697.1 ribosomal protein L23 [Passiflora obovata])

HSP 1 Score: 99.0 bits (245), Expect = 2.1e-17
Identity = 50/69 (72.46%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GI++  FTDK+ WLLGK QYT NVES    TEIKH V+LFFGVKV+AMNSHRLP K RRM
Sbjct: 3  GIQYAVFTDKSIWLLGKNQYTLNVESGSTRTEIKHWVELFFGVKVMAMNSHRLPGKGRRM 62

Query: 79 RPVMRHTMH 84
          RP+M HTMH
Sbjct: 63 RPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. NCBI nr
Match: YP_009474014.1 (ribosomal protein L23 [Itoa orientalis] >YP_009474034.1 ribosomal protein L23 [Itoa orientalis] >YP_009474099.1 ribosomal protein L23 [Poliothyrsis sinensis] >YP_009474119.1 ribosomal protein L23 [Poliothyrsis sinensis] >YP_009674617.1 ribosomal protein L23 [Carrierea calycina] >YP_009674638.1 ribosomal protein L23 [Carrierea calycina] >AVK40678.1 ribosomal protein L23 [Itoa orientalis] >AVK40698.1 ribosomal protein L23 [Itoa orientalis] >AVK40764.1 ribosomal protein L23 [Poliothyrsis sinensis] >AVK40782.1 ribosomal protein L23 [Poliothyrsis sinensis] >QDC34994.1 ribosomal protein L23 [Carrierea calycina])

HSP 1 Score: 99.0 bits (245), Expect = 2.1e-17
Identity = 52/69 (75.36%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+  LLGK QYTSNVES    TEIKH V+LFFGVKVIAMNSHRLP K RRM
Sbjct: 3  GIKYAVFTDKSILLLGKNQYTSNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKGRRM 62

Query: 79 RPVMRHTMH 84
          RP+M HTMH
Sbjct: 63 RPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. ExPASy Swiss-Prot
Match: Q7FNR4 (50S ribosomal protein L23, chloroplastic OS=Atropa belladonna OX=33113 GN=rpl23-A PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 7.9e-20
Identity = 52/69 (75.36%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+  LLGK QYTSNVES    TEIKH V+LFFGVKVIAMNSHRLP KSRRM
Sbjct: 3  GIKYAVFTDKSIRLLGKNQYTSNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKSRRM 62

Query: 79 RPVMRHTMH 84
           P+M HTMH
Sbjct: 63 GPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. ExPASy Swiss-Prot
Match: Q3C1N5 (50S ribosomal protein L23, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=rpl23-A PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 7.9e-20
Identity = 52/69 (75.36%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+  LLGK QYTSNVES    TEIKH V+LFFGVKVIAMNSHRLP KSRRM
Sbjct: 3  GIKYAVFTDKSIRLLGKNQYTSNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKSRRM 62

Query: 79 RPVMRHTMH 84
           P+M HTMH
Sbjct: 63 GPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. ExPASy Swiss-Prot
Match: Q33BY9 (50S ribosomal protein L23, chloroplastic OS=Nicotiana tomentosiformis OX=4098 GN=rpl23-A PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 7.9e-20
Identity = 52/69 (75.36%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+  LLGK QYTSNVES    TEIKH V+LFFGVKVIAMNSHRLP KSRRM
Sbjct: 3  GIKYAVFTDKSIRLLGKNQYTSNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKSRRM 62

Query: 79 RPVMRHTMH 84
           P+M HTMH
Sbjct: 63 GPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. ExPASy Swiss-Prot
Match: Q2MIE5 (50S ribosomal protein L23, chloroplastic OS=Solanum bulbocastanum OX=147425 GN=rpl23-A PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 7.9e-20
Identity = 52/69 (75.36%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+  LLGK QYTSNVES    TEIKH V+LFFGVKVIAMNSHRLP KSRRM
Sbjct: 3  GIKYAVFTDKSIRLLGKNQYTSNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKSRRM 62

Query: 79 RPVMRHTMH 84
           P+M HTMH
Sbjct: 63 GPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. ExPASy Swiss-Prot
Match: Q2MI58 (50S ribosomal protein L23, chloroplastic OS=Solanum lycopersicum OX=4081 GN=rpl23-A PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 7.9e-20
Identity = 52/69 (75.36%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+  LLGK QYTSNVES    TEIKH V+LFFGVKVIAMNSHRLP KSRRM
Sbjct: 3  GIKYAVFTDKSIRLLGKNQYTSNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKSRRM 62

Query: 79 RPVMRHTMH 84
           P+M HTMH
Sbjct: 63 GPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. ExPASy TrEMBL
Match: A0A6B9XLF4 (50S ribosomal protein L23, chloroplastic OS=Siphonostegia chinensis OX=374729 GN=rpl23 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 7.6e-18
Identity = 51/69 (73.91%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+ WLLGK QYTSNVES    TE+KH V+LFFGVKVIAMNSHRLP K RRM
Sbjct: 3  GIKNPVFTDKSIWLLGKNQYTSNVESGSTRTELKHWVELFFGVKVIAMNSHRLPGKGRRM 62

Query: 79 RPVMRHTMH 84
           P+M HTMH
Sbjct: 63 GPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. ExPASy TrEMBL
Match: A0A2P1CRM0 (50S ribosomal protein L23, chloroplastic OS=Itoa orientalis OX=179731 GN=rpl23 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-17
Identity = 52/69 (75.36%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+  LLGK QYTSNVES    TEIKH V+LFFGVKVIAMNSHRLP K RRM
Sbjct: 3  GIKYAVFTDKSILLLGKNQYTSNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKGRRM 62

Query: 79 RPVMRHTMH 84
          RP+M HTMH
Sbjct: 63 RPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. ExPASy TrEMBL
Match: A0A4Y5QGD7 (50S ribosomal protein L23, chloroplastic OS=Passiflora obovata OX=378248 GN=rpl23 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-17
Identity = 50/69 (72.46%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GI++  FTDK+ WLLGK QYT NVES    TEIKH V+LFFGVKV+AMNSHRLP K RRM
Sbjct: 3  GIQYAVFTDKSIWLLGKNQYTLNVESGSTRTEIKHWVELFFGVKVMAMNSHRLPGKGRRM 62

Query: 79 RPVMRHTMH 84
          RP+M HTMH
Sbjct: 63 RPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. ExPASy TrEMBL
Match: A0A6H1YDD7 (50S ribosomal protein L23, chloroplastic OS=Withania coagulans OX=180581 GN=rpl23 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-17
Identity = 53/69 (76.81%), Postives = 57/69 (82.61%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+V FTDK+  LLGK QYTSNVES    TEIKH V+LFFGVKVIAMNSHRLP KSRRM
Sbjct: 3  GIKYVVFTDKSIRLLGKNQYTSNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKSRRM 62

Query: 79 RPVMRHTMH 84
           P+M HTMH
Sbjct: 63 GPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. ExPASy TrEMBL
Match: A0A4Y5UR01 (50S ribosomal protein L23, chloroplastic OS=Carrierea calycina OX=312356 GN=rpl23 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.0e-17
Identity = 52/69 (75.36%), Postives = 56/69 (81.16%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+  LLGK QYTSNVES    TEIKH V+LFFGVKVIAMNSHRLP K RRM
Sbjct: 3  GIKYAVFTDKSILLLGKNQYTSNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKGRRM 62

Query: 79 RPVMRHTMH 84
          RP+M HTMH
Sbjct: 63 RPIMGHTMH 71

[Close]
BLAST of Carg13997 vs. TAIR 10
Match: ATCG00840.1 (ribosomal protein L23.1 )

HSP 1 Score: 90.5 bits (223), Expect = 6.8e-19
Identity = 48/69 (69.57%), Postives = 54/69 (78.26%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+  LLGK QYT NVES    TEIKH V+LFFGVKVIAMNSHRLP K +RM
Sbjct: 3  GIKYAVFTDKSIRLLGKNQYTFNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKVKRM 62

Query: 79 RPVMRHTMH 84
           P++ HTMH
Sbjct: 63 GPILGHTMH 71

[Close]
BLAST of Carg13997 vs. TAIR 10
Match: ATCG01300.1 (ribosomal protein L23 )

HSP 1 Score: 90.5 bits (223), Expect = 6.8e-19
Identity = 48/69 (69.57%), Postives = 54/69 (78.26%), Query Frame = 0 

Query: 19 GIKHVGFTDKTTWLLGKLQYTSNVES----TEIKHCVKLFFGVKVIAMNSHRLPKKSRRM 78
          GIK+  FTDK+  LLGK QYT NVES    TEIKH V+LFFGVKVIAMNSHRLP K +RM
Sbjct: 3  GIKYAVFTDKSIRLLGKNQYTFNVESGSTRTEIKHWVELFFGVKVIAMNSHRLPGKVKRM 62

Query: 79 RPVMRHTMH 84
           P++ HTMH
Sbjct: 63 GPILGHTMH 71

The following BLAST results are available for this feature:
BLAST of Carg13997 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7035768.16.6e-40100.0050S ribosomal protein L23, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
YP_009731796.11.6e-1773.91ribosomal protein L23 [Siphonostegia chinensis] >YP_009731816.1 ribosomal protei... [more]
YP_009756322.12.1e-1776.81ribosomal protein L23 [Withania coagulans] >YP_009756343.1 ribosomal protein L23... [more]
YP_009671421.12.1e-1772.46ribosomal protein L23 [Passiflora obovata] >YP_009671437.1 ribosomal protein L23... [more]
YP_009474014.12.1e-1775.36ribosomal protein L23 [Itoa orientalis] >YP_009474034.1 ribosomal protein L23 [I... [more]
BLAST of Carg13997 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q7FNR47.9e-2075.3650S ribosomal protein L23, chloroplastic OS=Atropa belladonna OX=33113 GN=rpl23-... [more]
Q3C1N57.9e-2075.3650S ribosomal protein L23, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=rpl2... [more]
Q33BY97.9e-2075.3650S ribosomal protein L23, chloroplastic OS=Nicotiana tomentosiformis OX=4098 GN... [more]
Q2MIE57.9e-2075.3650S ribosomal protein L23, chloroplastic OS=Solanum bulbocastanum OX=147425 GN=r... [more]
Q2MI587.9e-2075.3650S ribosomal protein L23, chloroplastic OS=Solanum lycopersicum OX=4081 GN=rpl2... [more]
BLAST of Carg13997 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6B9XLF47.6e-1873.9150S ribosomal protein L23, chloroplastic OS=Siphonostegia chinensis OX=374729 GN... [more]
A0A2P1CRM01.0e-1775.3650S ribosomal protein L23, chloroplastic OS=Itoa orientalis OX=179731 GN=rpl23 P... [more]
A0A4Y5QGD71.0e-1772.4650S ribosomal protein L23, chloroplastic OS=Passiflora obovata OX=378248 GN=rpl2... [more]
A0A6H1YDD71.0e-1776.8150S ribosomal protein L23, chloroplastic OS=Withania coagulans OX=180581 GN=rpl2... [more]
A0A4Y5UR011.0e-1775.3650S ribosomal protein L23, chloroplastic OS=Carrierea calycina OX=312356 GN=rpl2... [more]
BLAST of Carg13997 vs. TAIR 10
Match NameE-valueIdentityDescription
ATCG00840.16.8e-1969.57ribosomal protein L23.1 [more]
ATCG01300.16.8e-1969.57ribosomal protein L23 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012677Nucleotide-binding alpha-beta plait domain superfamilyGENE3D3.30.70.330coord: 19..83
e-value: 9.0E-7
score: 31.2
IPR013025Ribosomal protein L25/L23PANTHERPTHR1162060S RIBOSOMAL PROTEIN L23Acoord: 19..83
NoneNo IPR availablePANTHERPTHR11620:SF6950S RIBOSOMAL PROTEIN L23, CHLOROPLASTICcoord: 19..83
IPR012678Ribosomal protein L23/L15e core domain superfamilySUPERFAMILY54189Ribosomal proteins S24e, L23 and L15ecoord: 20..82

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13997-RACarg13997-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006412 translation
cellular_component GO:0009507 chloroplast
cellular_component GO:0005840 ribosome
molecular_function GO:0003729 mRNA binding
molecular_function GO:0019843 rRNA binding
molecular_function GO:0003735 structural constituent of ribosome