Carg13937 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg13937
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRING-type E3 ubiquitin transferase
LocationCarg_Chr08: 4357529 .. 4361799 (-)
RNA-Seq ExpressionCarg13937
SyntenyCarg13937
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTGGGTCGAAATTTGATGAATAATATTAACCAACCACTGCGACGGTGGTGGATTGCTCGAAGAAAAATTCCCTCCACTGCGCTACCCGTTTTCCCAAATTCTGCAGTTCTCCTCCGAATTGGCCTGCGTTATCTCCCGCCATTTGCCGGCAAATCGCCGCCGCACTGACTCGGCGGTTGTGCTGGTCGCCATCTACAACGCATTCAAGGAGGGAACTGTTTTCACCGCCACAGAGACGCGGAATTCACTGATGTCCTCATCGGAGCAAGCCCTCGTCTCTTTACTCTCCCGATTTGCTTTATCGTTTGATGGAGCTGCTTTGGGCGCAGCCCTAGCCTACGCTGCGTTTCGTACCGTCCTCAAACTCACTGCTTCCTGCTCTGCTCTCGATGAGCTCCGTAAAGCTCCAAATGTAAGAGTATCTGATCTTCGCCATATGATTTCCGATGGAGATTCGGGACAATCCGAGTCCGATCAAAAGCTTGTTGTAGTTCGTGGCACCGTGGAGGCCAGGTCGTTTGTGGAAGGTAATTGGAAGAGCTTGCAGCCTAATGTATTGATTTCTCAGGAGTCCGGAGATAGAGCTGTGATTATTCAGCGTACGCAAATGGTAAAATCTCCTATGGTATTTTTGCTAGGCACTGCGTGACTGTGTTAAGATGTTGTTTTTGGGGCTGCAGTTCTTCTGGGGAGGATAAGTGAAAACTGAGCTATGCAGTTTAGAGGATTGCCCGATTAAATTCAATATTATTCCGTTTGTTAGAATCATACGAATGCAGAGTTTTGTGTTAGTATTTGAGTTACTTGATAGCACTACTAAAAGTTGCCATTGAAGTCTAACCGATTAATCAATAACTCGTGAACTCGTGCATTGTTAAATTCTTTTCACATTATGAGGTCTCTAGTAACGTCCTTAAGTTCAGGAGCAATAATGATATGATTCTAGTCTGGATGAACAATGAAATGAGAAGAATCATGGGTGAAATTATATGATTCTAGTCTGCATAGGTCTTCTGGCTTGCCTGAATTTGTTCCATCATCATATATAATATATAACTACGATATGCGGATTAAACATTTGGGAAACCTTGAACTCTTAAATCAATTGTAAAATCTTGGGGAAAACGAATTTCTATTTTCTCAGACGAACTATCATAACCTAATTGGTTCTGCAAGTGTCTGTTTTTGTTCCCCTTTGCTATTCAGTATTGTGAATGTGTTGTGTGTGTACATAATATCCAGTTTTATAAACTAAAGCTTATTGGGTTGTAACTTGTGGCGTTGTTTAGTGCATATTCAACGAATGGAAGGGCTTTCTTGGATGGACTTCTGATTTACGTGCAATTTTTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCGTTCAGAACGGTAAAATCAAGCTTCTCTCTGTTATTGCTATGGTTGGTTCACTCTTTTTGTCCTTAGTAATAAAAGATCTTTGTATTCAATACCTTTTAAGCTTGAATAATATGGAACTTTGTTATTCTGTCTGTGAATAAAGAGCAAATTGAAGTTGAATGATTTTTTTCTGAGGGCATCTCTTATATGTGGCCCTTCTGTTAGGTCCTTTTCCTGTGATTGTCTATTGGATCTTCTTTTTCCCTTGCTTGTGGCGCGTGCAAGTAGGTTTGTAAGTGCTATCTCTTCAGAAAGGGGTCCAAACCGAGTAGGCTTGAAGGTATACCCCATAGTGTCAAGAAATAAGGAATTTATTGTAGCTAACTTTTTGTTTATAAAAACTAAATGTACTTCATATGAGATTGATGTGTGGCAGTTGTGATTTGTGAAGACTGAAGGTAAAATATATATTTTAGATATCTGGTTGAGCAGCAGGGAGCTATATTCCTGTGGTGATTGTACCAGAATTGAGTGCAATTTACATATTACGTCTTATCTTATGTGATGCTGATATTTGTGAAAAAATTTTACAGGTTCCTTTTGTCCTTGTTGAGGGTCATTCCCCGTATTCTGATTTTGTTGTTGTTAACATGGATGGCTCAAGACATCGTTTGCCTCTTACAACTGTGTATCGTCAACTGCAACCCATTTGCGCTACTCCTTATACCTTCCTTCAAGCACTTTTTGGTCATGAGTACCCTGTGAGTTAGGCAATCTATATTGAATTAAAACATCTTTTTCTTTCTTTTTATTAATGGATTTTATCTAAAAATTCTTTATCATGTTCTACTTTGAAGGTTGGGGTGCTTGATGAGGAGAAAATACTTTCATTAGGGAAAAATATCAGTGTTGTTGGCGTATGCAGTTTTAAAAATGGAGTTCCTGAGATAAAGTCAAGCAAGGATTTTCCCCACTTCCTGTAAGTTTATGCATTTTGAAGTAGAAATAACGTTGAGAAGGTCAAGATTATGTAATGTAATTTATGAAGAAGGTAAACCATTGATGCATATAATTAATTCTTTCCCTTGACTCATCCACATAAAATTTGACAATAAATGTGTTCTGTCTGTCAATAGTGAGCCCCATTAATTCGGCTTTAGTGCTCTGTTTGGGATTTTAACTAGGTCCTAGGATCAAGTCAATGAGTAGAAAGCTTTCGTTGTCTGTTGGAGTGATGCTAGCAAATGCTAGTCTCTCCCACATCCACTCGAAGTAACCCTCGCATGAGTGACTATGGGGAACAGTGTCTAATTGGACAAAAATTATTCGTGTTTGTCCTTTGAGATAATCTTCGTTGAAAAAACGGGTACTATGATGACTTACTTTTCCTTGCAGATGTGAAATGACTAAAGATCAGATGGTTTTAGATCTTGTCTTCAAAACAAAATTTCTGTTCTGGAGTAGTATTGTCCTCGGTTCGTTGTCAGTTGGCGTTCTTGGCTGTTCTGTTATGAGGTATGTTATCTTTCTTTCTTTCTGATACGTGGAAATCACTACAAGTGGAGTAAGATTCGGATTACCTTGTTGATCAAATATCTCAAGGCAAGAACATTGTTTGAGATTCGAATCACTCCACAAGCAAGATTGATCATGTCTAGTTTGAATGATTCTTGTTGATCAAATATCTCAACGCAAGAACCACTTGTTTGGGATTCGAATCACTCCACAAGCAAGATTGATCATGTCGAGCTTGAATGATTCTACATGCAACCTAACTACATAGAATTGCAAAGAAACTTAGCCATTGGCTAAAGAAAGCACTACATTTTTCAAGTCTACTTACAAATACAATATACATGGCTTTATATAGCCTCATAATGAAACTATTAAAGGCATTCCAAGAGTTGTAACATTCATACTTCATTATGTATATTGTAACCTAGAGTAAATAAAAAGTCCTAAAATATATTAATGAAATACAATAACTCTAAATTATTCTAAATTGTAATTTACCCAAAATTTATAACATGAAATTTCATTTTTCTTGAATGCAGCAATATTTTCTTCCTATCTTCATTAAAGTATATTGTATGATTGATGTCTCTTGGTTCATATCACTGTCTTTCTTTTATTTTATTGTCAAACTGTAGCAAGGTATCTTAATGTTCGGTAACTTTTTTAGGCAACGCAATGCACTCGTAATATATGCCATCCATGAAATGTTTATTTGCTTGGCACTCTGCTTTCTGCAAATTGGTAGGGTAAGGGATGAAGTAGAAGAGTAGCAATGATTTGTAGCAATGATTCTTCAATCTTTATTTTTCTAGTGGTGAATTTCTCCTGCTTTTATGAACTAACTAATGTTCTTATTTGGTCCTCGAATGGAATGAATAAAAGCTTAGCTCTACTGCGTAGTGAGTGAGTGATGAATGATGAGATTTGATGAATGTTTGAACTTATTAGCGCCCATTCTCTTTCAGAAATTGGAATAGATGGAAACAGTGGAGGCAACAGAGACGGTTGCAGGATTCAGGGAATGATTCCACCATGTCACAACTGGATTCAAGCAATAATATCCCCATCTATGGTGATTTTGGAACTGCATCAGACGACGAAGTAGCATCCATTCCAGATGACGAGTTATCATCGAACGTTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAGGCGATCTGCTTTCATTCCATGTGGTCATTTTGTCTGCTGTGAACGTTGTGCCATATCAGTCGAACGCGATTCAGCTCCCAAATGCCCCGTCTGTCGCCAGCAGATCCGTAGTTCTGTGCGAATATATGATTCGTGAGAAGAAGCAAATTATTTCTCAGGTTCGAACTCATCTATCTGGGAATCTCATTCATCTGGGTGGCCTTTTTTTTTTTTT

mRNA sequence

CTGGGTCGAAATTTGATGAATAATATTAACCAACCACTGCGACGGTGGTGGATTGCTCGAAGAAAAATTCCCTCCACTGCGCTACCCGTTTTCCCAAATTCTGCAGTTCTCCTCCGAATTGGCCTGCGTTATCTCCCGCCATTTGCCGGCAAATCGCCGCCGCACTGACTCGGCGGTTGTGCTGGTCGCCATCTACAACGCATTCAAGGAGGGAACTGTTTTCACCGCCACAGAGACGCGGAATTCACTGATGTCCTCATCGGAGCAAGCCCTCGTCTCTTTACTCTCCCGATTTGCTTTATCGTTTGATGGAGCTGCTTTGGGCGCAGCCCTAGCCTACGCTGCGTTTCGTACCGTCCTCAAACTCACTGCTTCCTGCTCTGCTCTCGATGAGCTCCGTAAAGCTCCAAATGTAAGAGTATCTGATCTTCGCCATATGATTTCCGATGGAGATTCGGGACAATCCGAGTCCGATCAAAAGCTTGTTGTAGTTCGTGGCACCGTGGAGGCCAGGTCGTTTGTGGAAGGTAATTGGAAGAGCTTGCAGCCTAATGTATTGATTTCTCAGGAGTCCGGAGATAGAGCTGTGATTATTCAGCGTACGCAAATGTGCATATTCAACGAATGGAAGGGCTTTCTTGGATGGACTTCTGATTTACGTGCAATTTTTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCGTTCAGAACGGTTCCTTTTGTCCTTGTTGAGGGTCATTCCCCGTATTCTGATTTTGTTGTTGTTAACATGGATGGCTCAAGACATCGTTTGCCTCTTACAACTGTGTATCGTCAACTGCAACCCATTTGCGCTACTCCTTATACCTTCCTTCAAGCACTTTTTGGTCATGAGTACCCTGTTGGGGTGCTTGATGAGGAGAAAATACTTTCATTAGGGAAAAATATCAGTGTTGTTGGCGTATGCAGTTTTAAAAATGGAGTTCCTGAGATAAAGTCAAGCAAGGATTTTCCCCACTTCCTATGTGAAATGACTAAAGATCAGATGGTTTTAGATCTTGTCTTCAAAACAAAATTTCTGTTCTGGAGTAGTATTGTCCTCGGTTCGTTGTCAGTTGGCGTTCTTGGCTGTTCTGTTATGAGAAATTGGAATAGATGGAAACAGTGGAGGCAACAGAGACGGTTGCAGGATTCAGGGAATGATTCCACCATGTCACAACTGGATTCAAGCAATAATATCCCCATCTATGGTGATTTTGGAACTGCATCAGACGACGAAGTAGCATCCATTCCAGATGACGAGTTATCATCGAACGTTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAGGCGATCTGCTTTCATTCCATGTGGTCATTTTGTCTGCTGTGAACGTTGTGCCATATCAGTCGAACGCGATTCAGCTCCCAAATGCCCCGTCTGTCGCCAGCAGATCCGTAGTTCTGTGCGAATATATGATTCGTGAGAAGAAGCAAATTATTTCTCAGGTTCGAACTCATCTATCTGGGAATCTCATTCATCTGGGTGGCCTTTTTTTTTTTTT

Coding sequence (CDS)

ATGTCCTCATCGGAGCAAGCCCTCGTCTCTTTACTCTCCCGATTTGCTTTATCGTTTGATGGAGCTGCTTTGGGCGCAGCCCTAGCCTACGCTGCGTTTCGTACCGTCCTCAAACTCACTGCTTCCTGCTCTGCTCTCGATGAGCTCCGTAAAGCTCCAAATGTAAGAGTATCTGATCTTCGCCATATGATTTCCGATGGAGATTCGGGACAATCCGAGTCCGATCAAAAGCTTGTTGTAGTTCGTGGCACCGTGGAGGCCAGGTCGTTTGTGGAAGGTAATTGGAAGAGCTTGCAGCCTAATGTATTGATTTCTCAGGAGTCCGGAGATAGAGCTGTGATTATTCAGCGTACGCAAATGTGCATATTCAACGAATGGAAGGGCTTTCTTGGATGGACTTCTGATTTACGTGCAATTTTTGGAAGAACCTTTAGAAAGCAAGAGTCAACATCGTTCAGAACGGTTCCTTTTGTCCTTGTTGAGGGTCATTCCCCGTATTCTGATTTTGTTGTTGTTAACATGGATGGCTCAAGACATCGTTTGCCTCTTACAACTGTGTATCGTCAACTGCAACCCATTTGCGCTACTCCTTATACCTTCCTTCAAGCACTTTTTGGTCATGAGTACCCTGTTGGGGTGCTTGATGAGGAGAAAATACTTTCATTAGGGAAAAATATCAGTGTTGTTGGCGTATGCAGTTTTAAAAATGGAGTTCCTGAGATAAAGTCAAGCAAGGATTTTCCCCACTTCCTATGTGAAATGACTAAAGATCAGATGGTTTTAGATCTTGTCTTCAAAACAAAATTTCTGTTCTGGAGTAGTATTGTCCTCGGTTCGTTGTCAGTTGGCGTTCTTGGCTGTTCTGTTATGAGAAATTGGAATAGATGGAAACAGTGGAGGCAACAGAGACGGTTGCAGGATTCAGGGAATGATTCCACCATGTCACAACTGGATTCAAGCAATAATATCCCCATCTATGGTGATTTTGGAACTGCATCAGACGACGAAGTAGCATCCATTCCAGATGACGAGTTATCATCGAACGTTCCAGATGGACAGTTGTGCGTGATCTGTCTCATGAGGAGAAGGCGATCTGCTTTCATTCCATGTGGTCATTTTGTCTGCTGTGAACGTTGTGCCATATCAGTCGAACGCGATTCAGCTCCCAAATGCCCCGTCTGTCGCCAGCAGATCCGTAGTTCTGTGCGAATATATGATTCGTGA

Protein sequence

MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDLRHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQMCIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHRLPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPEIKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS
Homology
BLAST of Carg13937 vs. NCBI nr
Match: KAG7025892.1 (E3 ubiquitin-protein ligase SPL2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 816.2 bits (2107), Expect = 1.2e-232
Identity = 407/407 (100.00%), Postives = 407/407 (100.00%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL
Sbjct: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180
           CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180

Query: 181 LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE 240
           LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE
Sbjct: 181 LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE 240

Query: 241 IKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300
           IKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR
Sbjct: 241 IKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360
           QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 408
           RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS
Sbjct: 361 RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Carg13937 vs. NCBI nr
Match: KAG6593550.1 (E3 ubiquitin-protein ligase SPL2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 812.8 bits (2098), Expect = 1.4e-231
Identity = 405/407 (99.51%), Postives = 405/407 (99.51%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSSSEQALVSL SRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL
Sbjct: 90  MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 149

Query: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 150 RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 209

Query: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180
           CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR
Sbjct: 210 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 269

Query: 181 LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE 240
           LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE
Sbjct: 270 LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE 329

Query: 241 IKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300
           IKS KDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR
Sbjct: 330 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 389

Query: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360
           QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM
Sbjct: 390 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 449

Query: 361 RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 408
           RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS
Sbjct: 450 RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 496

BLAST of Carg13937 vs. NCBI nr
Match: XP_023000260.1 (E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 803.1 bits (2073), Expect = 1.1e-228
Identity = 399/407 (98.03%), Postives = 402/407 (98.77%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSSSEQALVSL SRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHM SDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMTSDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180
           CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180

Query: 181 LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE 240
           LPLTTVYRQLQP+CATPYTFLQALFGH+YPVGVLDEEKIL LGKNISVVGVCSFKNGVPE
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHKYPVGVLDEEKILPLGKNISVVGVCSFKNGVPE 240

Query: 241 IKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300
           IKS KDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360
           QQRRLQDSGNDST+SQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTISQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 408
           RRRRSAFIPCGH VCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS
Sbjct: 361 RRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Carg13937 vs. NCBI nr
Match: XP_022964220.1 (E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 802.4 bits (2071), Expect = 1.9e-228
Identity = 399/407 (98.03%), Postives = 401/407 (98.53%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSSSEQALVSL SRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHMISDGDS QSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180
           CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDF VVNMDGSRHR
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFAVVNMDGSRHR 180

Query: 181 LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE 240
           LPLTTVYRQLQP+CATPYTFLQALFGHEYPVGVLDEEKIL LG+NISVVGVCSFKNGVPE
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPE 240

Query: 241 IKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300
           IKS KDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360
           QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 408
           RRRRSAFIPCGH VCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS
Sbjct: 361 RRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Carg13937 vs. NCBI nr
Match: XP_023514152.1 (E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 799.7 bits (2064), Expect = 1.2e-227
Identity = 398/407 (97.79%), Postives = 401/407 (98.53%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL
Sbjct: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHMISDGDS QSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180
           CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180

Query: 181 LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE 240
           LPLTTVYRQLQP+CATPYTFLQALFGHEYPVGVLDEEKIL LG+NISVVGVCSFKNGVPE
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPE 240

Query: 241 IKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300
           IKS KDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360
           QQRRLQDSGNDST+SQLDSSNN PIYGDFGTASDDE ASIPDDELSSNVPDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTISQLDSSNNDPIYGDFGTASDDEAASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 408
           RRRRSAFIPCGH VCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS
Sbjct: 361 RRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Carg13937 vs. ExPASy Swiss-Prot
Match: Q9SYH3 (E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.0e-96
Identity = 200/414 (48.31%), Postives = 268/414 (64.73%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSS E+AL++LL+  ALSFDGA LG  LA +A  + LK  ++ +AL +++ AP V +SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHMI------SDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVI 120
           R ++      S+ +  +  +DQ++VVVRG V+ +  + G+      NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPK--ISGDEGYKNNNVLISPETGDKALI 120

Query: 121 IQRTQMCIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLV-EGHSPYSDFVVVN 180
           IQRTQ  +++ WK     T   R +  R+ RK  +   RTVPFV+V +     S FV VN
Sbjct: 121 IQRTQTYVYSGWKRLFQSTGH-RFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSFVAVN 180

Query: 181 MDGSRHRLPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCS 240
           MDGSR  LPLTTVY +LQPI +   +FLQA    +YPVG+LD EKIL  GK+I+ VG+ S
Sbjct: 181 MDGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYS 240

Query: 241 FKNGVPEIKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNW 300
           F NGVPEIKS +D P+FL EMTKD+M+ DL+ +T F+F  S++LG +SVG+L  + +R W
Sbjct: 241 FNNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTW 300

Query: 301 NRWKQWRQQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQ 360
           N+WKQW  QR L    NDS +                   DDE    P+D  +  +PDG+
Sbjct: 301 NKWKQWNHQRELPQRPNDSVV-------------------DDE----PED--ADEIPDGE 360

Query: 361 LCVICLMRRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 408
           LCVIC+ RRR  AFIPCGH VCC RCA +VER+  PKCPVC Q IR S+R+Y S
Sbjct: 361 LCVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQSIRGSMRVYYS 383

BLAST of Carg13937 vs. ExPASy Swiss-Prot
Match: O08863 (Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE=1 SV=2)

HSP 1 Score: 60.5 bits (145), Expect = 5.2e-08
Identity = 28/84 (33.33%), Postives = 48/84 (57.14%), Query Frame = 0

Query: 322 NIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCGHFVCCERCAI 381
           +I +  D  +   D++A++P +E    + + ++C +C+ R     FIPCGH V C+ CA 
Sbjct: 520 DIFVQQDIRSLPTDDIAALPMEEQLRKLQEERMCKVCMDREVSIVFIPCGHLVVCKDCAP 579

Query: 382 SVERDSAPKCPVCRQQIRSSVRIY 406
           S+      KCP+CR  I+ +VR +
Sbjct: 580 SLR-----KCPICRGTIKGTVRTF 598

BLAST of Carg13937 vs. ExPASy Swiss-Prot
Match: Q94HV7 (E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.2e-07
Identity = 59/240 (24.58%), Postives = 113/240 (47.08%), Query Frame = 0

Query: 172 VNMDGSRHRLPLTTVYRQLQPICATPYTFLQALFGHEYP---VGVLDEEKILSLGKNISV 231
           VN+D S+  L L           A P + +Q   G+      +GV   E+++ +G  ++V
Sbjct: 120 VNVDVSQGELGLALTVGSDVFEKAEPVSLVQGALGYLKGFKILGVRHVERVVPIGTPLTV 179

Query: 232 VGVCSFKNGVPEIKSSK--DFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLG 291
           VG  + ++G+  ++  K    P ++  +  DQ++  L   ++   ++S+ L  L V ++ 
Sbjct: 180 VGE-AVRDGMGNVRIQKPEQGPFYVTYIPLDQLISKLGDLSRRFKYASMGLTVLGVILIS 239

Query: 292 CSVMRN-WNRWKQWRQQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDEL 351
             V+     R +   ++RR Q +     +    +    P+ G  GT+ D           
Sbjct: 240 KPVIEYILKRIEDTLERRRRQFA--LKRVVDAAARRAKPVTGG-GTSRD----------- 299

Query: 352 SSNVPDGQLCVICLMRRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIY 406
             + PD  LCV+CL ++  +AF+ CGH  CC  C++ +       CP+CR++I+  ++IY
Sbjct: 300 -GDTPD--LCVVCLDQKYNTAFVECGHMCCCTPCSLQLR-----TCPLCRERIQQVLKIY 336

BLAST of Carg13937 vs. ExPASy Swiss-Prot
Match: Q24306 (Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=Diap1 PE=1 SV=2)

HSP 1 Score: 58.5 bits (140), Expect = 2.0e-07
Identity = 26/62 (41.94%), Postives = 38/62 (61.29%), Query Frame = 0

Query: 346 SSNVPDGQLCVICLMRRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIY 405
           S+++P+ +LC IC      +AF+PCGH V C +CA SV      KCP+CR+     +R+Y
Sbjct: 382 STSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSV-----TKCPLCRKPFTDVMRVY 438

Query: 406 DS 408
            S
Sbjct: 442 FS 438

BLAST of Carg13937 vs. ExPASy Swiss-Prot
Match: P41436 (Apoptosis inhibitor IAP OS=Cydia pomonella granulosis virus (isolate Mexico/1963) OX=654905 GN=IAP PE=4 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.0e-07
Identity = 26/64 (40.62%), Postives = 38/64 (59.38%), Query Frame = 0

Query: 344 ELSSNVPDGQLCVICLMRRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVR 403
           E    V D +LC IC +      F+PCGH V C +CA+SV+     KCP+CR+ + S ++
Sbjct: 217 EKEPQVEDSKLCKICYVEECIVCFVPCGHVVACAKCALSVD-----KCPMCRKIVTSVLK 275

Query: 404 IYDS 408
           +Y S
Sbjct: 277 VYFS 275

BLAST of Carg13937 vs. ExPASy TrEMBL
Match: A0A6J1KHU7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494539 PE=4 SV=1)

HSP 1 Score: 803.1 bits (2073), Expect = 5.3e-229
Identity = 399/407 (98.03%), Postives = 402/407 (98.77%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSSSEQALVSL SRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHM SDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMTSDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180
           CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180

Query: 181 LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE 240
           LPLTTVYRQLQP+CATPYTFLQALFGH+YPVGVLDEEKIL LGKNISVVGVCSFKNGVPE
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHKYPVGVLDEEKILPLGKNISVVGVCSFKNGVPE 240

Query: 241 IKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300
           IKS KDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360
           QQRRLQDSGNDST+SQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTISQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 408
           RRRRSAFIPCGH VCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS
Sbjct: 361 RRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Carg13937 vs. ExPASy TrEMBL
Match: A0A6J1HK84 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304 PE=4 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 9.0e-229
Identity = 399/407 (98.03%), Postives = 401/407 (98.53%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSSSEQALVSL SRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHMISDGDS QSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFVVVNMDGSRHR 180
           CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDF VVNMDGSRHR
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLVEGHSPYSDFAVVNMDGSRHR 180

Query: 181 LPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPE 240
           LPLTTVYRQLQP+CATPYTFLQALFGHEYPVGVLDEEKIL LG+NISVVGVCSFKNGVPE
Sbjct: 181 LPLTTVYRQLQPVCATPYTFLQALFGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPE 240

Query: 241 IKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300
           IKS KDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR
Sbjct: 241 IKSCKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWR 300

Query: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360
           QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM
Sbjct: 301 QQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLM 360

Query: 361 RRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 408
           RRRRSAFIPCGH VCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS
Sbjct: 361 RRRRSAFIPCGHLVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Carg13937 vs. ExPASy TrEMBL
Match: A0A6J1KJE8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494539 PE=4 SV=1)

HSP 1 Score: 780.0 bits (2013), Expect = 4.8e-222
Identity = 399/456 (87.50%), Postives = 402/456 (88.16%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSSSEQALVSL SRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHM SDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMTSDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRT-------------------------- 180
           CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRT                          
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVKSSFSLLLLWSFSCDSLLDLLFPLL 180

Query: 181 -----------------------VPFVLVEGHSPYSDFVVVNMDGSRHRLPLTTVYRQLQ 240
                                  VPFVLVEGHSPYSDFVVVNMDGSRHRLPLTTVYRQLQ
Sbjct: 181 VARASRFVSAISSERGPNRVGLKVPFVLVEGHSPYSDFVVVNMDGSRHRLPLTTVYRQLQ 240

Query: 241 PICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPEIKSSKDFPHFL 300
           P+CATPYTFLQALFGH+YPVGVLDEEKIL LGKNISVVGVCSFKNGVPEIKS KDFPHFL
Sbjct: 241 PVCATPYTFLQALFGHKYPVGVLDEEKILPLGKNISVVGVCSFKNGVPEIKSCKDFPHFL 300

Query: 301 CEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGND 360
           CEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGND
Sbjct: 301 CEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGND 360

Query: 361 STMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCG 408
           ST+SQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCG
Sbjct: 361 STISQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCG 420

BLAST of Carg13937 vs. ExPASy TrEMBL
Match: A0A6J1HH89 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304 PE=4 SV=1)

HSP 1 Score: 779.2 bits (2011), Expect = 8.1e-222
Identity = 399/456 (87.50%), Postives = 401/456 (87.94%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSSSEQALVSL SRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL
Sbjct: 1   MSSSEQALVSLFSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60

Query: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RHMISDGDS QSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHMISDGDSRQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRT-------------------------- 180
           CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRT                          
Sbjct: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVKFSFSLLLLWSFSCDSYWIFFFPLL 180

Query: 181 -----------------------VPFVLVEGHSPYSDFVVVNMDGSRHRLPLTTVYRQLQ 240
                                  VPFVLVEGHSPYSDF VVNMDGSRHRLPLTTVYRQLQ
Sbjct: 181 VARASRFVSAISSERGPNRVGLKVPFVLVEGHSPYSDFAVVNMDGSRHRLPLTTVYRQLQ 240

Query: 241 PICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVPEIKSSKDFPHFL 300
           P+CATPYTFLQALFGHEYPVGVLDEEKIL LG+NISVVGVCSFKNGVPEIKS KDFPHFL
Sbjct: 241 PVCATPYTFLQALFGHEYPVGVLDEEKILPLGRNISVVGVCSFKNGVPEIKSCKDFPHFL 300

Query: 301 CEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGND 360
           CEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGND
Sbjct: 301 CEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQWRQQRRLQDSGND 360

Query: 361 STMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCG 408
           STMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCG
Sbjct: 361 STMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPCG 420

BLAST of Carg13937 vs. ExPASy TrEMBL
Match: A0A6J1JT39 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 PE=4 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.3e-211
Identity = 374/408 (91.67%), Postives = 388/408 (95.10%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSSSEQALVSLLS+FALSFDGA LGAALAYAA RTVLKLTASCSALDELRKAP V VSDL
Sbjct: 1   MSSSEQALVSLLSQFALSFDGAVLGAALAYAAVRTVLKLTASCSALDELRKAPYVSVSDL 60

Query: 61  RHMISDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVIIQRTQM 120
           RH+ISDGDSG+SESD KLVVVRGTVEARS VEGNWKS+QPNVLISQESGDRAVIIQRTQM
Sbjct: 61  RHIISDGDSGKSESDHKLVVVRGTVEARSSVEGNWKSVQPNVLISQESGDRAVIIQRTQM 120

Query: 121 CIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLV-EGHSPYSDFVVVNMDGSRH 180
           CIFNEWKGF GWTSDLRAIFGRTFRKQESTSFRTVPFVLV EGHSPYSDFVVVNMDGSRH
Sbjct: 121 CIFNEWKGFFGWTSDLRAIFGRTFRKQESTSFRTVPFVLVDEGHSPYSDFVVVNMDGSRH 180

Query: 181 RLPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCSFKNGVP 240
            LPLTTVY QLQP+ ATPYTFLQALFGHEYPVGVLDEEKIL LGKNIS VG+CSFKNGVP
Sbjct: 181 PLPLTTVYHQLQPVSATPYTFLQALFGHEYPVGVLDEEKILPLGKNISAVGICSFKNGVP 240

Query: 241 EIKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNWNRWKQW 300
            IKS KDFPHFLCEMTKDQM+LDLVFKTKFLFWSS+VLGSLSVGVLG SV+RNWNRWKQW
Sbjct: 241 VIKSCKDFPHFLCEMTKDQMILDLVFKTKFLFWSSVVLGSLSVGVLGYSVVRNWNRWKQW 300

Query: 301 RQQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICL 360
           RQQRRLQDSGNDSTMSQLD SNNIPIYGDFGTASD+E A  P+DE+SSNVPDGQLCVICL
Sbjct: 301 RQQRRLQDSGNDSTMSQLD-SNNIPIYGDFGTASDNEEAVDPEDEVSSNVPDGQLCVICL 360

Query: 361 MRRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 408
           MRR+RSAFIPCGHFVCCERCA+SVERDSAPKCPVCRQQIRSSVRIYDS
Sbjct: 361 MRRKRSAFIPCGHFVCCERCAMSVERDSAPKCPVCRQQIRSSVRIYDS 407

BLAST of Carg13937 vs. TAIR 10
Match: AT1G54150.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 355.1 bits (910), Expect = 7.3e-98
Identity = 200/414 (48.31%), Postives = 268/414 (64.73%), Query Frame = 0

Query: 1   MSSSEQALVSLLSRFALSFDGAALGAALAYAAFRTVLKLTASCSALDELRKAPNVRVSDL 60
           MSS E+AL++LL+  ALSFDGA LG  LA +A  + LK  ++ +AL +++ AP V +SDL
Sbjct: 1   MSSPERALLNLLTDIALSFDGAILGLTLAVSAVGSALKYASTNAALKKIKDAPEVSISDL 60

Query: 61  RHMI------SDGDSGQSESDQKLVVVRGTVEARSFVEGNWKSLQPNVLISQESGDRAVI 120
           R ++      S+ +  +  +DQ++VVVRG V+ +  + G+      NVLIS E+GD+A+I
Sbjct: 61  RSLLPASEDKSETNDNRKSNDQRIVVVRGVVKPK--ISGDEGYKNNNVLISPETGDKALI 120

Query: 121 IQRTQMCIFNEWKGFLGWTSDLRAIFGRTFRKQESTSFRTVPFVLV-EGHSPYSDFVVVN 180
           IQRTQ  +++ WK     T   R +  R+ RK  +   RTVPFV+V +     S FV VN
Sbjct: 121 IQRTQTYVYSGWKRLFQSTGH-RFMLERSLRKHGADFTRTVPFVIVGKDQQSNSSFVAVN 180

Query: 181 MDGSRHRLPLTTVYRQLQPICATPYTFLQALFGHEYPVGVLDEEKILSLGKNISVVGVCS 240
           MDGSR  LPLTTVY +LQPI +   +FLQA    +YPVG+LD EKIL  GK+I+ VG+ S
Sbjct: 181 MDGSRQPLPLTTVYNRLQPINS---SFLQAFLYPDYPVGLLDIEKILPPGKDITAVGIYS 240

Query: 241 FKNGVPEIKSSKDFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLGCSVMRNW 300
           F NGVPEIKS +D P+FL EMTKD+M+ DL+ +T F+F  S++LG +SVG+L  + +R W
Sbjct: 241 FNNGVPEIKSCQDLPYFLSEMTKDKMIEDLMEQTNFIFLGSVILGIVSVGILSYAAVRTW 300

Query: 301 NRWKQWRQQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQ 360
           N+WKQW  QR L    NDS +                   DDE    P+D  +  +PDG+
Sbjct: 301 NKWKQWNHQRELPQRPNDSVV-------------------DDE----PED--ADEIPDGE 360

Query: 361 LCVICLMRRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIYDS 408
           LCVIC+ RRR  AFIPCGH VCC RCA +VER+  PKCPVC Q IR S+R+Y S
Sbjct: 361 LCVICVSRRRVPAFIPCGHVVCCRRCASTVERELNPKCPVCLQSIRGSMRVYYS 383

BLAST of Carg13937 vs. TAIR 10
Match: AT1G59560.1 (E3 Ubiquitin ligase family protein )

HSP 1 Score: 59.3 bits (142), Expect = 8.3e-09
Identity = 59/240 (24.58%), Postives = 113/240 (47.08%), Query Frame = 0

Query: 172 VNMDGSRHRLPLTTVYRQLQPICATPYTFLQALFGHEYP---VGVLDEEKILSLGKNISV 231
           VN+D S+  L L           A P + +Q   G+      +GV   E+++ +G  ++V
Sbjct: 120 VNVDVSQGELGLALTVGSDVFEKAEPVSLVQGALGYLKGFKILGVRHVERVVPIGTPLTV 179

Query: 232 VGVCSFKNGVPEIKSSK--DFPHFLCEMTKDQMVLDLVFKTKFLFWSSIVLGSLSVGVLG 291
           VG  + ++G+  ++  K    P ++  +  DQ++  L   ++   ++S+ L  L V ++ 
Sbjct: 180 VGE-AVRDGMGNVRIQKPEQGPFYVTYIPLDQLISKLGDLSRRFKYASMGLTVLGVILIS 239

Query: 292 CSVMRN-WNRWKQWRQQRRLQDSGNDSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDEL 351
             V+     R +   ++RR Q +     +    +    P+ G  GT+ D           
Sbjct: 240 KPVIEYILKRIEDTLERRRRQFA--LKRVVDAAARRAKPVTGG-GTSRD----------- 299

Query: 352 SSNVPDGQLCVICLMRRRRSAFIPCGHFVCCERCAISVERDSAPKCPVCRQQIRSSVRIY 406
             + PD  LCV+CL ++  +AF+ CGH  CC  C++ +       CP+CR++I+  ++IY
Sbjct: 300 -GDTPD--LCVVCLDQKYNTAFVECGHMCCCTPCSLQLR-----TCPLCRERIQQVLKIY 336

BLAST of Carg13937 vs. TAIR 10
Match: AT4G24204.1 (RING/U-box superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 5.4e-08
Identity = 33/94 (35.11%), Postives = 44/94 (46.81%), Query Frame = 0

Query: 311 DSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPC 370
           D T   ++ S  +   GD  TAS            S +V    LCVIC   RR   F+PC
Sbjct: 34  DWTPPLINESLEVTGKGDVETASFSS---------SDDVDYSTLCVICFEERRNCFFVPC 93

Query: 371 GHFVCCERCAISVERDSAPKCPVCRQQIRSSVRI 405
           GH   C  CA  +  + +  CP+CR+ IR S R+
Sbjct: 94  GHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 118

BLAST of Carg13937 vs. TAIR 10
Match: AT4G24204.2 (RING/U-box superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 5.4e-08
Identity = 33/94 (35.11%), Postives = 44/94 (46.81%), Query Frame = 0

Query: 311 DSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPC 370
           D T   ++ S  +   GD  TAS            S +V    LCVIC   RR   F+PC
Sbjct: 68  DWTPPLINESLEVTGKGDVETASFSS---------SDDVDYSTLCVICFEERRNCFFVPC 127

Query: 371 GHFVCCERCAISVERDSAPKCPVCRQQIRSSVRI 405
           GH   C  CA  +  + +  CP+CR+ IR S R+
Sbjct: 128 GHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 152

BLAST of Carg13937 vs. TAIR 10
Match: AT4G24204.3 (RING/U-box superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 5.4e-08
Identity = 33/94 (35.11%), Postives = 44/94 (46.81%), Query Frame = 0

Query: 311 DSTMSQLDSSNNIPIYGDFGTASDDEVASIPDDELSSNVPDGQLCVICLMRRRRSAFIPC 370
           D T   ++ S  +   GD  TAS            S +V    LCVIC   RR   F+PC
Sbjct: 89  DWTPPLINESLEVTGKGDVETASFSS---------SDDVDYSTLCVICFEERRNCFFVPC 148

Query: 371 GHFVCCERCAISVERDSAPKCPVCRQQIRSSVRI 405
           GH   C  CA  +  + +  CP+CR+ IR S R+
Sbjct: 149 GHSATCRGCAQRILSEESKVCPICRRVIRKSKRL 173

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025892.11.2e-232100.00E3 ubiquitin-protein ligase SPL2 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6593550.11.4e-23199.51E3 ubiquitin-protein ligase SPL2, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_023000260.11.1e-22898.03E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita maxima][more]
XP_022964220.11.9e-22898.03E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita moschata][more]
XP_023514152.11.2e-22797.79E3 ubiquitin-protein ligase SPL2-like isoform X2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SYH31.0e-9648.31E3 ubiquitin-protein ligase SPL2 OS=Arabidopsis thaliana OX=3702 GN=SPL2 PE=2 SV... [more]
O088635.2e-0833.33Baculoviral IAP repeat-containing protein 3 OS=Mus musculus OX=10090 GN=Birc3 PE... [more]
Q94HV71.2e-0724.58E3 ubiquitin-protein ligase SPL1 OS=Arabidopsis thaliana OX=3702 GN=SPL1 PE=2 SV... [more]
Q243062.0e-0741.94Death-associated inhibitor of apoptosis 1 OS=Drosophila melanogaster OX=7227 GN=... [more]
P414362.0e-0740.63Apoptosis inhibitor IAP OS=Cydia pomonella granulosis virus (isolate Mexico/1963... [more]
Match NameE-valueIdentityDescription
A0A6J1KHU75.3e-22998.03RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494539 P... [more]
A0A6J1HK849.0e-22998.03RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304... [more]
A0A6J1KJE84.8e-22287.50RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111494539 P... [more]
A0A6J1HH898.1e-22287.50RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111464304... [more]
A0A6J1JT391.3e-21191.67RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488642 P... [more]
Match NameE-valueIdentityDescription
AT1G54150.17.3e-9848.31E3 Ubiquitin ligase family protein [more]
AT1G59560.18.3e-0924.58E3 Ubiquitin ligase family protein [more]
AT4G24204.15.4e-0835.11RING/U-box superfamily protein [more]
AT4G24204.25.4e-0835.11RING/U-box superfamily protein [more]
AT4G24204.35.4e-0835.11RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 405..407
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 353..400
e-value: 3.7E-14
score: 52.3
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 349..401
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 355..394
e-value: 0.0033
score: 26.7
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 355..395
score: 10.704165
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 330..406
e-value: 5.4E-17
score: 63.0
IPR022170E3 Ubiquitin ligase MUL1-likePFAMPF12483GIDEcoord: 142..285
e-value: 5.6E-33
score: 113.6
IPR044247E3 ubiquitin-protein ligase SPL2-likePANTHERPTHR47355E3 UBIQUITIN-PROTEIN LIGASE SPL2coord: 1..407

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg13937-RACarg13937-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006996 organelle organization
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0009941 chloroplast envelope
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016874 ligase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity