Carg13450 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg13450
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type E3 ubiquitin transferase
Location	Carg_Chr08: 2901304 .. 2907098 (+)
RNA-Seq Expression	Carg13450
Synteny	Carg13450

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTCCGAACGAACAATGAAGTGCGTGGAATTCGTTCTTTTTTCCACTTTCCATTTCCCTCCACTGCTTATATCTTATTCAAATCTTCTCTTACTTGTTTAATCAACCTTTTCAATCGTCTTTTCTTCCATTTTCCGCTTCTAACTTCGATCGCCATGTCTAAAAGGTGAGGTTCTGTTCTTTTGTTTCGATTCTGCTTCTTTCTATGTTCTTGTTGATTTTGTCTGGCAGATGCTTGTTTGTTCGTTTTCTCGTGTATTTTTTAAAAGTTTTGATGATTGGATGGCAGATTACGCGCTTTGATTCTTTTTTTCTTTTTCTTGAATAGTATCTTCCGGTTCATGGTTTTCTTTGTTTGGATGTACCTTCTCGAGTAATCTTGATCATGGGTCTTTGGTATGTGGTTTGATTATTAGGTGTATGCTATTTTGAGGGGCTGGGTGATCAAATATGTGGTTGGAAATGTTTGGAATTTGAGTTTCACGAAAGCTGTTTTGTTGGGTGGTTGTTGTTGAGGCTGGACTTTGTGTTTATGAAGTTTTGTTATTTTATATCTTGGAATTAACACGTTCTTAGCGTCTTAATTTACTTTGGTGGATTGTTTAGGGTTCTATGCAAGTATTTTGCGCATGGAGCATGCTTGAAAGGGGAGTATTGTGAGTTTTCGCATGACTGGACGGCTCCACCTAGCAATGTAAATATTACTTCCACTCCTCTGCATTGCTATGCATTTTAATGTAAACCAACTGTCAGTTTGGCACTCCGATCATTCTTTCTTTCTGATGTATCATTCGTGAAACAGGTTTGCACTTACTATCAAAAGGGAAGGTGTGCTTTTGGCAGTCGATGCAGATATGACCATGTTAAAGTTTCTCATGCAGAGTCATCAGCTTCTTCTAATTCTCAGCAACCTCACGTGTCTGAATCTGTTTCAATAGCTCATACTCTATTATCAAGTCCACTTTCTCCTTCACCTGGGTCGACAATTGAGCTTGCTGGTTCAAAGATATCCTGTCTTCCCTCGCACGTTGAATCTGCTTGGAGTGTAGAGTGTGAGCTTCCATCTGGTCTTGATGATTGGGGGAGTAGGGAGACAAGGATTATTAGTCCAGCCGATTTACCTATTTGCTCTTTTTCTGCTGCTGGTACTAATTGTCCGCGTGGTGATAATTGCCCTCACATTCATGGGGACTTATGTCCCACGTGCGGAAAACAATGTTTACATCCTTTTAGGACGGATGAAAGGGAGGAGCATATGAAGGCATGTGAGAAAAAGCAAAAACATCTCGAGTCTTTGAAACATAGCCAAGAAATAGAGTGTAGTGTCTGCCTCGAGCGTGTTCTTTCTAAAACCGAAATAGCCGAGAGGAAGTTTGGGCTTCTATCTGAATGTGATCATCCGTTCTGCATTTCTTGTATTAGAAACTGGCGTAATAGTTCATCTACCTCCGGAATGGATGTTAACAGTGCTTTAAGAGCCTGCCCAATTTGTAGGAAGCTATCATACTTTGTTATTCCAAGCGTCATTTGGTATTCTTCCAAGGAAGAAAAACAAGAGATCGTTGATAGCTACAAGTCAAAGCTGCGGTAAATTTATGTTCTTAATTCCTAATATTTGATTGCATTCTGGATACTATTAACTTTTGTCCATTCTGTTCCCATTTTTTATGTTTTATAACCAAACTCATTTTTGTCTATGCCATATCAAATGTAATCTGGAAAGCTCCATAGTCCACCGAATGATCAATATGCTTTCTAGAAGAATGGAAACTGCATAACTGTTCTAAAGTAATTGTGTGAGCTGTTGAAATAATTTCTATACTCTGATCTTGCAACTTTATATTATTTTCATGCACAGATCAATCGATTGCAAGCACTTTAACTTTGGAAATGGGAACTGCCCATTTGGATCTAGCTGTTTTTACAAGGTAAATTTTTCTCTGAAATCATCTACTTCAGTGTTCTTATCCTGATTGTGCTCTTCTAAGCAAGCTGGATGTTGCTGTAGCCTTTGGAATTGCAATTTTCCTGAAATTTTTTCTTCTTCCTTTTTTTTTTTTCCCCCTTTATTCCTTTATCCATATTGCTTTTTAACCGATCATTGATATGCTGAATGGAAATCTCAACCTGTTTGGTTGTTCTAATATTTGAATGCCTATGAAGTATGTTGATTGGTTACATCTTTGATTTCATGGACATGAGTTTATGGAAGGAATTCTTTTTGTGATCTGGAGACGGAAATATAGTGCAACAGAAGATTCTTTTTGTTCTTATGATCCTCCAATGGTTTTATAAGCGTCTCCATTTACCATGGCCATCCAATCATGGTGGGTTAGATATGTGGAATCGTACAATTTGAACTTGCATGTTGACAAAGTAAATTTGCTAATCATTTGGCCAGCTTTTTCACTATGTTTTTGTGCAATTTTCTTGCTATTAGGTGCTCATAATAATAGACTACCAATATGATGAAAAATATATAAATTTCTGGTTCAGTACCTCTTAGTGTGTTTCATTTTGTTCTTTTGACACAATGGTAAGGGTGCTTCTTTCTTGTCCATTTTTTTTTAAACAAAACCAAAGGATGCCTCCATTTCTCATAGGTATCCTCTCTTGGGAAGCCCTGTTTCAGTTAGAATAGTTCGAGGGCGAGGATGAGGGGTGAAGTCCCTTTCTTTGCTCCCACCGGTGTTCCTACTATAACCAAATCTCATAAATAATAACTTGCTTTGGTGTATACTATAGATAATCTATATCCATTATCTTTTCGTTTTCTGTTTCTAAAATTCATGCTTTATAACCAATTTCACTGTTCTGTAATCTATTTTTAAAAATCCTAAACAAATTATTTTTAGAAATTGTGCTTGATGGGAACTAAGAATTTAAAAGTGCTTTTAAAATGTGATAAACAAAAAAATGTATGATCTTAAAATTTACTTGAGTCTTTGAATAACAATTTTCTTTAGTCTTCAATTTAGTTTTGAGGATATTTTAAAAGCCTACCTTTTTTTTTTAATTAAAAATAAAATGTAAAGAATATTTTTAATAGTTAAAAGAAAACCCTCCAAATTTTGGTTTTATGTATTTAAAATTTGGCTGAGAATTTTAAACTGGCTTTTGAAAAGCAGAAAACAAAAAAACTAAACTAAGAAACGGTTGGTTAATTTTCGAAAAATTAAAATTCTATGTTTATTAAGCAAGGCCTAAGTTTTATCTACAAATGTCCATGTAATTAGTGGGGATGATTTCACAATTTCTCAAACAAAATCTTTGCAATTACTTTTGTTGCCGTCTTAGAAAATTTTCTGGTCTCAATCTGGCTGAAAGTGATGTGCTTACTACTTTGGCTGATTGGCTCTGCAACCTCTGTTTAGTAATGTCTTATCAGTCTTTTAGCTAAATTACCAATATGATGAAATTATTTAATTTTGCAAACCAATATTTTATTGCTAGACCTCCTATAAAAAAAAGAAAAAGAATGTATTGCTAGACCTGTTATAATTGATTGGTCTGTTTTAAGGTGAAGATAATAGACCTCTCTGTTTCAAATTAATAAAATTAATATACTTATAGCTATTGGATTTAAAAGTGTTATCATGAGGATATTGTAGGTTAGCACCATAGAATGACTGGAGGGACCTCTTCTAAATTGCGTTCTTTTAATTAGGAAAACTGTTATTGGAATCATTCATCAGTTCTAAAGCCAATCCATATGCTTGCAAATCATTGGCGCGTGTCTATTTTGGAGAATAAATATGCAACTGCTCTGTCACATTCTTCTGTCTCCCAAAAACTTTCAGTCAGCTTTTGTGGTAAATATTGAAAGATGTGGTAGACATAAGCAGTTCTGTTCATTGCGCATTCTGTTGTCATTTAATTTCATTAGAGCAGATTGGCTTTCCAATCTTTTGATTGATACAATGTTTGTTGTGTTCCAGCACTTGGTCAAGCCTGGTTCATACACATGGAAATATCACAGGCCACCTCTGCTCCGACCACGATCACAGAGAACTCCTCCTAGCAGATCTAATATTGTGGATATGGATGCCTTTTTTGATATGTTTGAAGACTTAGAGGATTTGGATTTTAGTGCTGAAGATTTAGATTTTGAAGATTTAACACCTTTGGAGATGGCATTTGTTCTAATGCAGTTGGGTTGTGAAGCCAACTTGTCAAGTGATGAGGAGAATTATAGTTTTTGAACTACATTTTTTATAGGTAGTTGTGCCTGATGTAAATTGGGTCTTTCACGAGTTCCTTTCTTGTCTTTGCCCAGATATTTGCTATTCTAGCATTTTTGGCCACATAGCAGTGCCCTACGTGCAAAAATACAGGCTCGTAGACTTTGTTAATTGTCACAAACGCCATTATTGGGGAATGATACTTCACAATAAGCTTTAGTCATTTGTCCTAGCGCAAATTAGTTGCTGAGTTACCACGATGACGATGATCTATATATGGGAGACCCCTTTTTGTTATGTGTAGATTATACTATGACTTCGGAAGTGTTGTATGTTACCGCTGGGAATTAGGAATATCTGTTACTTGCTATCTTTCATCATTCATAGATTTTATGCGGAGTTTTGTTTCACGCTCTTGCTTTCCCAAGCCAATTGTGCTCTCCTCATGTTTGATTTGGTGATTTCTTTGTTTGCAGTTGTGAAGTGTTTTTTTTTCTTTTCCTTCGAATATTTGGATTCTTTACTTCCCAAATCTTATATGTCATTTTCTGCCTTTGAAGTGTTTGCAATTCTGCCTTTTAGTCTGCTGTATTAACTTCAAAATGAGATACAAATTTTGATCTGATTTTCTCATTGCAGCACGCACATCGGGATGGCCGGTTGGAAGAGGTAGCACTGCGTCACATTGGGAATCAAGACGGCCTCACTATTATAGCTAAAAACATTAGGTTTTGTTTTGTGCATTTGCTTGATATTGTCCATTTCTTGTGCAATTTCGCCCTTTATTAATTTGATGCTATTTCCAGGTTGTCGGACTTCCTTAGTGACTTGCATATCAGGTGAAAGAACCATATTATCAGGTATGAAAACTCACGAAGCAGCCCTCACCCCTAAGCTCAGCTTCATGCCATTTGCCGTCTACTGTATGATATTTTCACATTTTAATATGATAATCATTGAATCTTGAAAACTTATGTTGTTGCTAGTCATAAAGTCTTGTTTGGACTTTGAAATTCAGCATCTTTATCAATTGACACCTAATATATTTATAAGAAACGGAGTTTATTAATCTTGGTACACAACCTGAGATCACTCTATTTCAAACTCGTTCTTGTTCCATATGTAACACTCTTGTCCATGAAATGCCAAACTTTGTGCATTGCATTAGTCTCGAGTTAGGCTTGCTTAGGAAATCAGTTATTAACAATTTAGTCCATGAAATGCCAAACTTTGTGCATTTCATTAGGCTTGAGTTAGGCTTGTATTAACGATTTAGTCCATGAAATGCCGAACTCTGTACTCTGTGTATTGTTGTAAAGTCTTCTTTTACAGAAATCTAAGAGTAGTTTGAAAACGCGTTTCAGGTGTCGAATTGCTTGCTGGAGTCCGATAATGAGAAATGGAAGGTGGGTTATTGAACACGGTGATTCGAACTTCATCATCTTCTATGAATGGAAATAAAGCTAAAGACATTGTCATACTGAGAAATGAATGTAGTAAGTTGAAGCCTTATTCTTGGGATGGCAAAATAAAGGGTTTTTCACATCAGATGTAGCACAGCTGAATAGCTCAATAAATTGCGTCGTTGGTTGTACGTGCTGTACTAAACATTC

mRNA sequence

CTTCTCCGAACGAACAATGAAGTGCGTGGAATTCGTTCTTTTTTCCACTTTCCATTTCCCTCCACTGCTTATATCTTATTCAAATCTTCTCTTACTTGTTTAATCAACCTTTTCAATCGTCTTTTCTTCCATTTTCCGCTTCTAACTTCGATCGCCATGTCTAAAAGGGTTCTATGCAAGTATTTTGCGCATGGAGCATGCTTGAAAGGGGAGTATTGTGAGTTTTCGCATGACTGGACGGCTCCACCTAGCAATGTTTGCACTTACTATCAAAAGGGAAGGTGTGCTTTTGGCAGTCGATGCAGATATGACCATGTTAAAGTTTCTCATGCAGAGTCATCAGCTTCTTCTAATTCTCAGCAACCTCACGTGTCTGAATCTGTTTCAATAGCTCATACTCTATTATCAAGTCCACTTTCTCCTTCACCTGGGTCGACAATTGAGCTTGCTGGTTCAAAGATATCCTGTCTTCCCTCGCACGTTGAATCTGCTTGGAGTGTAGAGTGTGAGCTTCCATCTGGTCTTGATGATTGGGGGAGTAGGGAGACAAGGATTATTAGTCCAGCCGATTTACCTATTTGCTCTTTTTCTGCTGCTGGTACTAATTGTCCGCGTGGTGATAATTGCCCTCACATTCATGGGGACTTATGTCCCACGTGCGGAAAACAATGTTTACATCCTTTTAGGACGGATGAAAGGGAGGAGCATATGAAGGCATGTGAGAAAAAGCAAAAACATCTCGAGTCTTTGAAACATAGCCAAGAAATAGAGTGTAGTGTCTGCCTCGAGCGTGTTCTTTCTAAAACCGAAATAGCCGAGAGGAAGTTTGGGCTTCTATCTGAATGTGATCATCCGTTCTGCATTTCTTGTATTAGAAACTGGCGTAATAGTTCATCTACCTCCGGAATGGATGTTAACAGTGCTTTAAGAGCCTGCCCAATTTGTAGGAAGCTATCATACTTTGTTATTCCAAGCGTCATTTGGTATTCTTCCAAGGAAGAAAAACAAGAGATCGTTGATAGCTACAAGTCAAAGCTGCGATCAATCGATTGCAAGCACTTTAACTTTGGAAATGGGAACTGCCCATTTGGATCTAGCTGTTTTTACAAGCACTTGGTCAAGCCTGGTTCATACACATGGAAATATCACAGGCCACCTCTGCTCCGACCACGATCACAGAGAACTCCTCCTAGCAGATCTAATATTGTGGATATGGATGCCTTTTTTGATATGTTTGAAGACTTAGAGGATTTGGATTTTAGTGCTGAAGATTTAGATTTTGAAGATTTAACACCTTTGGAGATGGCATTTGTTCTAATGCAGTTGGGTTGTGAAGCCAACTTGTCAAGTGATGAGGAGAATTATATTGTGCCTGATCACGCACATCGGGATGGCCGGTTGGAAGAGGTAGCACTGCGTCACATTGGGAATCAAGACGGCCTCACTATTATAGCTAAAAACATTAGGTTGTCGGACTTCCTTAGTGACTTGCATATCAGGTGTCGAATTGCTTGCTGGAGTCCGATAATGAGAAATGGAAGGTGGGTTATTGAACACGGTGATTCGAACTTCATCATCTTCTATGAATGGAAATAAAGCTAAAGACATTGTCATACTGAGAAATGAATGTAGTAAGTTGAAGCCTTATTCTTGGGATGGCAAAATAAAGGGTTTTTCACATCAGATGTAGCACAGCTGAATAGCTCAATAAATTGCGTCGTTGGTTGTACGTGCTGTACTAAACATTC

Coding sequence (CDS)

ATGTCTAAAAGGGTTCTATGCAAGTATTTTGCGCATGGAGCATGCTTGAAAGGGGAGTATTGTGAGTTTTCGCATGACTGGACGGCTCCACCTAGCAATGTTTGCACTTACTATCAAAAGGGAAGGTGTGCTTTTGGCAGTCGATGCAGATATGACCATGTTAAAGTTTCTCATGCAGAGTCATCAGCTTCTTCTAATTCTCAGCAACCTCACGTGTCTGAATCTGTTTCAATAGCTCATACTCTATTATCAAGTCCACTTTCTCCTTCACCTGGGTCGACAATTGAGCTTGCTGGTTCAAAGATATCCTGTCTTCCCTCGCACGTTGAATCTGCTTGGAGTGTAGAGTGTGAGCTTCCATCTGGTCTTGATGATTGGGGGAGTAGGGAGACAAGGATTATTAGTCCAGCCGATTTACCTATTTGCTCTTTTTCTGCTGCTGGTACTAATTGTCCGCGTGGTGATAATTGCCCTCACATTCATGGGGACTTATGTCCCACGTGCGGAAAACAATGTTTACATCCTTTTAGGACGGATGAAAGGGAGGAGCATATGAAGGCATGTGAGAAAAAGCAAAAACATCTCGAGTCTTTGAAACATAGCCAAGAAATAGAGTGTAGTGTCTGCCTCGAGCGTGTTCTTTCTAAAACCGAAATAGCCGAGAGGAAGTTTGGGCTTCTATCTGAATGTGATCATCCGTTCTGCATTTCTTGTATTAGAAACTGGCGTAATAGTTCATCTACCTCCGGAATGGATGTTAACAGTGCTTTAAGAGCCTGCCCAATTTGTAGGAAGCTATCATACTTTGTTATTCCAAGCGTCATTTGGTATTCTTCCAAGGAAGAAAAACAAGAGATCGTTGATAGCTACAAGTCAAAGCTGCGATCAATCGATTGCAAGCACTTTAACTTTGGAAATGGGAACTGCCCATTTGGATCTAGCTGTTTTTACAAGCACTTGGTCAAGCCTGGTTCATACACATGGAAATATCACAGGCCACCTCTGCTCCGACCACGATCACAGAGAACTCCTCCTAGCAGATCTAATATTGTGGATATGGATGCCTTTTTTGATATGTTTGAAGACTTAGAGGATTTGGATTTTAGTGCTGAAGATTTAGATTTTGAAGATTTAACACCTTTGGAGATGGCATTTGTTCTAATGCAGTTGGGTTGTGAAGCCAACTTGTCAAGTGATGAGGAGAATTATATTGTGCCTGATCACGCACATCGGGATGGCCGGTTGGAAGAGGTAGCACTGCGTCACATTGGGAATCAAGACGGCCTCACTATTATAGCTAAAAACATTAGGTTGTCGGACTTCCTTAGTGACTTGCATATCAGGTGTCGAATTGCTTGCTGGAGTCCGATAATGAGAAATGGAAGGTGGGTTATTGAACACGGTGATTCGAACTTCATCATCTTCTATGAATGGAAATAA

Protein sequence

MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELPSGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMFEDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVALRHIGNQDGLTIIAKNIRLSDFLSDLHIRCRIACWSPIMRNGRWVIEHGDSNFIIFYEWK

Homology

[Close]

BLAST of Carg13450 vs. NCBI nr
Match: KAG7025660.1 (putative RING-type E3 ubiquitin transferase C3H69 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1011.1 bits (2613), Expect = 3.1e-291
Identity = 479/479 (100.00%), Postives = 479/479 (100.00%), Query Frame = 0


Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP
Sbjct: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
           SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVAL 420
           EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVAL
Sbjct: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVAL 420

Query: 421 RHIGNQDGLTIIAKNIRLSDFLSDLHIRCRIACWSPIMRNGRWVIEHGDSNFIIFYEWK 480
           RHIGNQDGLTIIAKNIRLSDFLSDLHIRCRIACWSPIMRNGRWVIEHGDSNFIIFYEWK
Sbjct: 421 RHIGNQDGLTIIAKNIRLSDFLSDLHIRCRIACWSPIMRNGRWVIEHGDSNFIIFYEWK 479

[Close]

BLAST of Carg13450 vs. NCBI nr
Match: XP_022959900.1 (E3 ubiquitin-protein ligase makorin-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 840.5 bits (2170), Expect = 7.2e-240
Identity = 400/403 (99.26%), Postives = 401/403 (99.50%), Query Frame = 0


Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGS+ISCLPSHVESAWSVECELP
Sbjct: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSRISCLPSHVESAWSVECELP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
           SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 403

[Close]

BLAST of Carg13450 vs. NCBI nr
Match: XP_023514215.1 (E3 ubiquitin-protein ligase makorin-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 838.6 bits (2165), Expect = 2.7e-239
Identity = 400/403 (99.26%), Postives = 400/403 (99.26%), Query Frame = 0


Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           SSASSNSQQPHVSESVSIAHTLLSSPLSPSP STIELAGSKISCLPSHVESAWSVECELP
Sbjct: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPESTIELAGSKISCLPSHVESAWSVECELP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
           SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 403

[Close]

BLAST of Carg13450 vs. NCBI nr
Match: XP_023004552.1 (E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 836.3 bits (2159), Expect = 1.4e-238
Identity = 397/403 (98.51%), Postives = 400/403 (99.26%), Query Frame = 0


Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVK SHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKASHAE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           SSASSNSQQPH+SESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP
Sbjct: 61  SSASSNSQQPHMSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
           SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPHRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           +DLEDLDFSAEDLDFE+LTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 361 QDLEDLDFSAEDLDFEELTPLEMAFVLMQLGCEANLSSDEENY 403

[Close]

BLAST of Carg13450 vs. NCBI nr
Match: XP_022959901.1 (E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 832.8 bits (2150), Expect = 1.5e-237
Identity = 396/399 (99.25%), Postives = 397/399 (99.50%), Query Frame = 0


Query: 5   VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSAS 64
           VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSAS
Sbjct: 5   VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSAS 64

Query: 65  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELPSGLD 124
           SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGS+ISCLPSHVESAWSVECELPSGLD
Sbjct: 65  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSRISCLPSHVESAWSVECELPSGLD 124

Query: 125 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 184
           DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH
Sbjct: 125 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 184

Query: 185 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 244
           MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN
Sbjct: 185 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 244

Query: 245 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 304
           SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF
Sbjct: 245 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 304

Query: 305 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMFEDLE 364
           GNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMFEDLE
Sbjct: 305 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMFEDLE 364

Query: 365 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 365 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 403

[Close]

BLAST of Carg13450 vs. ExPASy Swiss-Prot
Match: E0X9N4 (Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=3702 GN=At3g63550 PE=3 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.4e-124
Identity = 232/448 (51.79%), Postives = 285/448 (63.62%), Query Frame = 0


Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKR+LCK+FAHGACLKGE+CEFSHDW  P +N+CTYYQKG C++GSRCRY+HVK S   
Sbjct: 1   MSKRILCKFFAHGACLKGEHCEFSHDWKDPTNNICTYYQKGICSYGSRCRYEHVKASRPH 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLP-SHVESAWSVECEL 120
            SASS+S  P  S + S ++TL  + L   PG    +A   +S +P S ++  W+++   
Sbjct: 61  PSASSSSAVPRPSPA-SASNTLACAFL---PG----VAERDLSPVPSSSLKPTWNLDSLH 120

Query: 121 PSGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTD 180
              +D+  +     + P +LPICSF+AAG +CPRG+ CPHIHG +CPTCGK CLHPFR D
Sbjct: 121 QDSVDEVKTSNPGTVKPEELPICSFAAAG-DCPRGNECPHIHGTICPTCGKCCLHPFRPD 180

Query: 181 EREEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCI 240
           EREEH K CEKK K LE+LK SQEIEC VCLERVLSK   AERKFGLL+ECDH FCI+CI
Sbjct: 181 EREEHKKVCEKKHKQLEALKLSQEIECCVCLERVLSKATPAERKFGLLTECDHAFCIACI 240

Query: 241 RNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDC 300
           RNWR+SS ++GMDVNS LRACPICRKLSYFV+PSVIW+S+ EEK+EI+D+Y+ KLRSIDC
Sbjct: 241 RNWRSSSPSTGMDVNSTLRACPICRKLSYFVVPSVIWFSAPEEKKEIMDNYREKLRSIDC 300

Query: 301 KHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDM 360
           KHF+FG+GNCPFG+SCFYK                                         
Sbjct: 301 KHFSFGDGNCPFGTSCFYK----------------------------------------- 350

Query: 361 FEDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVA 420
                                                           HA+ DGRLEEV 
Sbjct: 361 ------------------------------------------------HAYHDGRLEEVV 350

Query: 421 LRHIGNQDGLTIIAKNIRLSDFLSDLHI 448
           LRH+  +DG T+IAK+IRLSDFL ++HI
Sbjct: 421 LRHLDAEDGQTVIAKDIRLSDFLENMHI 350

[Close]

BLAST of Carg13450 vs. ExPASy Swiss-Prot
Match: Q5ZA07 (E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=MKRN PE=2 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 2.2e-122
Identity = 239/457 (52.30%), Postives = 270/457 (59.08%), Query Frame = 0


Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAES 61
           +KRVLCK+F HGACLKGEYCEFSHDW   P+NVCT+YQKG C++GSRCRYDHVKVS   +
Sbjct: 3   TKRVLCKFFMHGACLKGEYCEFSHDWNDQPNNVCTFYQKGSCSYGSRCRYDHVKVSRNPT 62

Query: 62  SASSNSQQPHVSESVSIAHTLLSSPL-------SPSPGSTIELAGSKISCLPSHVESAWS 121
            A   S       S S+       P        S +P   I +     S       + + 
Sbjct: 63  VAPPPSSSTTTRASSSLQPLSFGRPHHVGYQADSSNPRQQISMDVLAHSGSKPVWRNDFQ 122

Query: 122 VECELPSGLDDWGSRET----RIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGK 181
            E  L  G+ DW    T      +SPADLPICSF AAG NCP G+ CP +HGDLC TCGK
Sbjct: 123 HESVLEDGI-DWSISPTVQNQTTLSPADLPICSF-AAGGNCPYGEECPQMHGDLCTTCGK 182

Query: 182 QCLHPFRTDEREEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSEC 241
            CLHP+R DEREEH K CEK  K LESLK SQEIECSVCL+RVLSK   AERKFGLLSEC
Sbjct: 183 MCLHPYRPDEREEHTKLCEKNHKRLESLKRSQEIECSVCLDRVLSKPTAAERKFGLLSEC 242

Query: 242 DHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSY 301
           DHPFCISCIRNWRN+S TSGMDVNSALRACPICRKLSY+VIPSV+WY SKEEK EI+D+Y
Sbjct: 243 DHPFCISCIRNWRNNSPTSGMDVNSALRACPICRKLSYYVIPSVLWYFSKEEKLEIIDNY 302

Query: 302 KSKLRSIDCKHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNI 361
           K+KL+SIDCK+F+FG G CPFGSSCFYK                                
Sbjct: 303 KAKLKSIDCKYFDFGTGTCPFGSSCFYK-------------------------------- 362

Query: 362 VDMDAFFDMFEDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAH 421
                                                                    HA+
Sbjct: 363 ---------------------------------------------------------HAY 368

Query: 422 RDGRLEEVALRHIGNQDGLTIIAKNIRLSDFLSDLHI 448
           RDGRLEEV LRH+   DG T+IAKNIRLSDFLS LH+
Sbjct: 423 RDGRLEEVILRHLDADDGSTVIAKNIRLSDFLSRLHL 368

[Close]

BLAST of Carg13450 vs. ExPASy Swiss-Prot
Match: Q6IDS6 (E3 ubiquitin-protein ligase makorin OS=Arabidopsis thaliana OX=3702 GN=MKRN PE=2 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 3.7e-114
Identity = 219/447 (48.99%), Postives = 263/447 (58.84%), Query Frame = 0


Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GSRCRYDHV+     
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVR----- 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
             A+SN      SES       L   +S +P   ++  G       +  + + +V C   
Sbjct: 61  --AASNLPLSSDSES-------LDRSISTTPSRHLQQQGDN-----NDGDKSSNVYC--- 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
                        I P + PICSF+AAG +CPRG+ CPH+HGDLC TCGK+CLHPFR +E
Sbjct: 121 -------------IHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH K CEKKQKH+E+LK SQ+IECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIR
Sbjct: 181 REEHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWR+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK+KLRSIDCK
Sbjct: 241 NWRSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFG+SCFYK                                          
Sbjct: 301 HFNFGNGNCPFGASCFYK------------------------------------------ 322

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVAL 420
                                                          HA+ DG LEEV L
Sbjct: 361 -----------------------------------------------HAYSDGHLEEVVL 322

Query: 421 RHIGNQDGLTIIAKNIRLSDFLSDLHI 448
           RH+G+Q+G T+I  +IRLS+FL  L I
Sbjct: 421 RHLGSQEGETVITDSIRLSEFLGGLQI 322

[Close]

BLAST of Carg13450 vs. ExPASy Swiss-Prot
Match: Q8JFF3 (Probable E3 ubiquitin-protein ligase makorin-1 OS=Seriola quinqueradiata OX=8161 GN=mkrn1 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 8.2e-61
Identity = 140/439 (31.89%), Postives = 225/439 (51.25%), Query Frame = 0


Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWT--APPSNVCTYYQKGRCAFGSRCRYDHVKVSHA 61
           +K V C+YF HG C +G+ C +SHD T   P + +C ++QKG C FG RCR++H K    
Sbjct: 19  TKHVTCRYFMHGLCKEGDNCRYSHDLTNSKPAAMICKFFQKGNCVFGDRCRFEHCK---- 78

Query: 62  ESSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSK-----ISCLPSHVESAWS 121
               + N + P   + + +    L+ P  P P     + G++        +P       +
Sbjct: 79  ---PAKNEELP-APQMLPLPSASLAGPSDPEPSGPTPVPGAQDWVNAAEFVPGQPYCGRA 138

Query: 122 VECELPSG---LDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQ 181
            + ++ S    ++++ S            +C ++A G  C  G NC ++HGD+C  CG Q
Sbjct: 139 EQAKVESSVPLIEEFDSYPAPDNKQLRKQLCPYAAVG-ECRYGINCAYLHGDVCYMCGLQ 198

Query: 182 CLHPFRTDEREEHMKAC-EKKQKHLE---SLKHSQEIECSVCLERVLSKTEIAERKFGLL 241
            LHP   ++R EH KAC E  +K +E   +++ S+++ C VC+E V  K   +ER+FG+L
Sbjct: 199 VLHPTDNNQRSEHTKACIEAHEKDMEISFAIQRSKDMMCGVCMEVVFEKANPSERRFGIL 258

Query: 242 SECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIV 301
           S C H +C+ CIR WR++        +  +++CP CR  S FVIPS  W   K++KQ+++
Sbjct: 259 SNCSHCYCLKCIRKWRSAKQFE----SKIIKSCPECRITSNFVIPSEYWVEDKDDKQKLI 318

Query: 302 DSYKSKLRSIDCKHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRT-PPS 361
             YK  + S  C++F+ G G CPFGS+CFYKH    G       R    +P+ ++T   S
Sbjct: 319 QKYKDGMGSKPCRYFDEGRGTCPFGSNCFYKHAFPDG-------RLEEAQPQRRQTGSNS 378

Query: 362 RSNIVDMDAFFDMFEDLEDLD-FSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIV 421
           R+        +D++++ E  D F  ED +       EM  +L+  G      +D+E  I+
Sbjct: 379 RNRNSRRTPLWDIYDERESTDSFDNEDEEMVTFELSEMLLMLLAAG------TDDEEVII 431

Query: 422 --PDHAHRDGRLEEVALRH 423
             P  A   GRL+    R+
Sbjct: 439 RPPSCATSSGRLDPTVTRY 431

[Close]

BLAST of Carg13450 vs. ExPASy Swiss-Prot
Match: Q4VBT5 (Probable E3 ubiquitin-protein ligase makorin-1 OS=Danio rerio OX=7955 GN=mkrn1 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.1e-60
Identity = 141/422 (33.41%), Postives = 217/422 (51.42%), Query Frame = 0


Query: 2   SKRVLCKYFAHGACLKGEYCEFSHDWTA-PPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 61
           +K V C+YF HG C +GE C +SHD ++   + +C ++QKG CAFG RCRY+H K S  +
Sbjct: 19  TKHVTCRYFMHGLCKEGENCRYSHDLSSCKQTMICKFFQKGCCAFGDRCRYEHTKPSKQD 78

Query: 62  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIEL--------------AGSKISCLP 121
              SS    P    +  +A T    P+S  PG T                 A   +   P
Sbjct: 79  EVPSSKPSMP--LTAAPLAGT--PEPVSDGPGGTTGAQEKPQGSGAVDWVNAAEFVPGQP 138

Query: 122 SHVESAWSVECELPSGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCP 181
            +   A  V CE P  L +    + +        +C ++A G  C  G NC ++HGD+C 
Sbjct: 139 -YCGRADPVLCEGPGPLIEEEYEKEQANKEMKKQLCPYAAVG-ECRYGLNCAYLHGDVCD 198

Query: 182 TCGKQCLHPFRTDEREEHMKAC-EKKQKHLE---SLKHSQEIECSVCLERVLSKTEIAER 241
            CG Q LHP  T +R +H++AC E  +K +E   +++ S+++ C VC+E V  KT  +ER
Sbjct: 199 MCGLQVLHPSDTSQRSQHIRACIEAHEKDMEISFAIQRSKDMMCGVCMEVVFEKTNPSER 258

Query: 242 KFGLLSECDHPFCISCIRNWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEE 301
           +FG+LS C H +C+ CIR WR++        +  +++CP CR  S FVIPS  W   KEE
Sbjct: 259 RFGILSNCCHCYCLKCIRKWRSAKQFE----SKIIKSCPECRITSNFVIPSEYWVEDKEE 318

Query: 302 KQEIVDSYKSKLRSIDCKHFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQR 361
           KQ+++  YK  + +  C++F+ G G CPFG++CFYKH    G    +   P     R Q 
Sbjct: 319 KQQLIQKYKDGMGTKPCRYFDEGRGTCPFGANCFYKHAFPDG----RLEEPQ--PQRRQN 378

Query: 362 TPPSRSNIVDMDAFFDMFEDLEDLD-FSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEE 404
               R+        +D+ ++ E+ D F  ED +       EM  +L+  G + +++  E+
Sbjct: 379 GSNGRNRNTRRTHLWDLLDERENSDSFDNEDEEMVRFELSEMLLMLLAAGTDDDVTDSED 424

[Close]

BLAST of Carg13450 vs. ExPASy TrEMBL
Match: A0A6J1H7L6 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460818 PE=4 SV=1)

HSP 1 Score: 840.5 bits (2170), Expect = 3.5e-240
Identity = 400/403 (99.26%), Postives = 401/403 (99.50%), Query Frame = 0


Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGS+ISCLPSHVESAWSVECELP
Sbjct: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSRISCLPSHVESAWSVECELP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
           SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 403

[Close]

BLAST of Carg13450 vs. ExPASy TrEMBL
Match: A0A6J1KQR4 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111497821 PE=4 SV=1)

HSP 1 Score: 836.3 bits (2159), Expect = 6.6e-239
Identity = 397/403 (98.51%), Postives = 400/403 (99.26%), Query Frame = 0


Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVK SHAE
Sbjct: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKASHAE 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
           SSASSNSQQPH+SESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP
Sbjct: 61  SSASSNSQQPHMSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
           SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE
Sbjct: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR
Sbjct: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK
Sbjct: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMF
Sbjct: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPHRPRSQRTPPSRSNIVDMDAFFDMF 360

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           +DLEDLDFSAEDLDFE+LTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 361 QDLEDLDFSAEDLDFEELTPLEMAFVLMQLGCEANLSSDEENY 403

[Close]

BLAST of Carg13450 vs. ExPASy TrEMBL
Match: A0A6J1H659 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460818 PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 7.3e-238
Identity = 396/399 (99.25%), Postives = 397/399 (99.50%), Query Frame = 0


Query: 5   VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSAS 64
           VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSAS
Sbjct: 5   VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSAS 64

Query: 65  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELPSGLD 124
           SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGS+ISCLPSHVESAWSVECELPSGLD
Sbjct: 65  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSRISCLPSHVESAWSVECELPSGLD 124

Query: 125 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 184
           DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH
Sbjct: 125 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 184

Query: 185 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 244
           MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN
Sbjct: 185 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 244

Query: 245 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 304
           SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF
Sbjct: 245 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 304

Query: 305 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMFEDLE 364
           GNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMFEDLE
Sbjct: 305 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMFEDLE 364

Query: 365 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 365 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 403

[Close]

BLAST of Carg13450 vs. ExPASy TrEMBL
Match: A0A6J1H5V2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460818 PE=4 SV=1)

HSP 1 Score: 832.8 bits (2150), Expect = 7.3e-238
Identity = 396/399 (99.25%), Postives = 397/399 (99.50%), Query Frame = 0


Query: 5   VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSAS 64
           VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSAS
Sbjct: 3   VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSAS 62

Query: 65  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELPSGLD 124
           SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGS+ISCLPSHVESAWSVECELPSGLD
Sbjct: 63  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSRISCLPSHVESAWSVECELPSGLD 122

Query: 125 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 184
           DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH
Sbjct: 123 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 182

Query: 185 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 244
           MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN
Sbjct: 183 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 242

Query: 245 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 304
           SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF
Sbjct: 243 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 302

Query: 305 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMFEDLE 364
           GNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMFEDLE
Sbjct: 303 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPPRPRSQRTPPSRSNIVDMDAFFDMFEDLE 362

Query: 365 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 363 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 401

[Close]

BLAST of Carg13450 vs. ExPASy TrEMBL
Match: A0A6J1KSE8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111497821 PE=4 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.4e-236
Identity = 393/399 (98.50%), Postives = 396/399 (99.25%), Query Frame = 0


Query: 5   VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAESSAS 64
           VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVK SHAESSAS
Sbjct: 20  VLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKASHAESSAS 79

Query: 65  SNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELPSGLD 124
           SNSQQPH+SESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELPSGLD
Sbjct: 80  SNSQQPHMSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELPSGLD 139

Query: 125 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 184
           DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH
Sbjct: 140 DWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDEREEH 199

Query: 185 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 244
           MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN
Sbjct: 200 MKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIRNWRN 259

Query: 245 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 304
           SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF
Sbjct: 260 SSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCKHFNF 319

Query: 305 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMFEDLE 364
           GNGNCPFGSSCFYKHLVKPGSYTWKYHRPP  RPRSQRTPPSRSNIVDMDAFFDMF+DLE
Sbjct: 320 GNGNCPFGSSCFYKHLVKPGSYTWKYHRPPPHRPRSQRTPPSRSNIVDMDAFFDMFQDLE 379

Query: 365 DLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENY 404
           DLDFSAEDLDFE+LTPLEMAFVLMQLGCEANLSSDEENY
Sbjct: 380 DLDFSAEDLDFEELTPLEMAFVLMQLGCEANLSSDEENY 418

[Close]

BLAST of Carg13450 vs. TAIR 10
Match: AT3G08505.2 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 2.7e-115
Identity = 219/447 (48.99%), Postives = 263/447 (58.84%), Query Frame = 0


Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GSRCRYDHV+     
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVR----- 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
             A+SN      SES       L   +S +P   ++  G       +  + + +V C   
Sbjct: 61  --AASNLPLSSDSES-------LDRSISTTPSRHLQQQGDN-----NDGDKSSNVYC--- 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
                        I P + PICSF+AAG +CPRG+ CPH+HGDLC TCGK+CLHPFR +E
Sbjct: 121 -------------IHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH K CEKKQKH+E+LK SQ+IECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIR
Sbjct: 181 REEHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWR+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK+KLRSIDCK
Sbjct: 241 NWRSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFG+SCFYK                                          
Sbjct: 301 HFNFGNGNCPFGASCFYK------------------------------------------ 322

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVAL 420
                                                          HA+ DG LEEV L
Sbjct: 361 -----------------------------------------------HAYSDGHLEEVVL 322

Query: 421 RHIGNQDGLTIIAKNIRLSDFLSDLHI 448
           RH+G+Q+G T+I  +IRLS+FL  L I
Sbjct: 421 RHLGSQEGETVITDSIRLSEFLGGLQI 322

[Close]

BLAST of Carg13450 vs. TAIR 10
Match: AT3G08505.1 (zinc finger (CCCH-type/C3HC4-type RING finger) family protein )

HSP 1 Score: 413.3 bits (1061), Expect = 2.7e-115
Identity = 219/447 (48.99%), Postives = 263/447 (58.84%), Query Frame = 0


Query: 1   MSKRVLCKYFAHGACLKGEYCEFSHDWTAPPSNVCTYYQKGRCAFGSRCRYDHVKVSHAE 60
           MS R+LCK+F HG+CLKGE CEFSHD   PP+NVCT+YQK  C +GSRCRYDHV+     
Sbjct: 1   MSDRILCKFFVHGSCLKGENCEFSHDSKDPPNNVCTFYQKRICLYGSRCRYDHVR----- 60

Query: 61  SSASSNSQQPHVSESVSIAHTLLSSPLSPSPGSTIELAGSKISCLPSHVESAWSVECELP 120
             A+SN      SES       L   +S +P   ++  G       +  + + +V C   
Sbjct: 61  --AASNLPLSSDSES-------LDRSISTTPSRHLQQQGDN-----NDGDKSSNVYC--- 120

Query: 121 SGLDDWGSRETRIISPADLPICSFSAAGTNCPRGDNCPHIHGDLCPTCGKQCLHPFRTDE 180
                        I P + PICSF+AAG +CPRG+ CPH+HGDLC TCGK+CLHPFR +E
Sbjct: 121 -------------IHPREYPICSFAAAG-DCPRGNQCPHMHGDLCNTCGKKCLHPFRPEE 180

Query: 181 REEHMKACEKKQKHLESLKHSQEIECSVCLERVLSKTEIAERKFGLLSECDHPFCISCIR 240
           REEH K CEKKQKH+E+LK SQ+IECSVCL+R+LSK    ERKFGLL+ECDHPFCI CIR
Sbjct: 181 REEHTKECEKKQKHIEALKQSQDIECSVCLDRILSKATPGERKFGLLTECDHPFCIQCIR 240

Query: 241 NWRNSSSTSGMDVNSALRACPICRKLSYFVIPSVIWYSSKEEKQEIVDSYKSKLRSIDCK 300
           NWR+S+  SGMDVNS LRACPICRKLSYFV+PSV+WYSS EEK+EI+D YK+KLRSIDCK
Sbjct: 241 NWRSSAPVSGMDVNSTLRACPICRKLSYFVVPSVVWYSSPEEKKEIIDIYKAKLRSIDCK 300

Query: 301 HFNFGNGNCPFGSSCFYKHLVKPGSYTWKYHRPPLLRPRSQRTPPSRSNIVDMDAFFDMF 360
           HFNFGNGNCPFG+SCFYK                                          
Sbjct: 301 HFNFGNGNCPFGASCFYK------------------------------------------ 322

Query: 361 EDLEDLDFSAEDLDFEDLTPLEMAFVLMQLGCEANLSSDEENYIVPDHAHRDGRLEEVAL 420
                                                          HA+ DG LEEV L
Sbjct: 361 -----------------------------------------------HAYSDGHLEEVVL 322

Query: 421 RHIGNQDGLTIIAKNIRLSDFLSDLHI 448
           RH+G+Q+G T+I  +IRLS+FL  L I
Sbjct: 421 RHLGSQEGETVITDSIRLSEFLGGLQI 322

The following BLAST results are available for this feature:

BLAST of Carg13450 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7025660.1	3.1e-291	100.00	putative RING-type E3 ubiquitin transferase C3H69 [Cucurbita argyrosperma subsp....	[more]
XP_022959900.1	7.2e-240	99.26	E3 ubiquitin-protein ligase makorin-like isoform X1 [Cucurbita moschata]	[more]
XP_023514215.1	2.7e-239	99.26	E3 ubiquitin-protein ligase makorin-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023004552.1	1.4e-238	98.51	E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita maxima]	[more]
XP_022959901.1	1.5e-237	99.25	E3 ubiquitin-protein ligase makorin-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Carg13450 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
E0X9N4	1.4e-124	51.79	Putative RING-type E3 ubiquitin transferase C3H69 OS=Arabidopsis thaliana OX=370...	[more]
Q5ZA07	2.2e-122	52.30	E3 ubiquitin-protein ligase makorin OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
Q6IDS6	3.7e-114	48.99	E3 ubiquitin-protein ligase makorin OS=Arabidopsis thaliana OX=3702 GN=MKRN PE=2...	[more]
Q8JFF3	8.2e-61	31.89	Probable E3 ubiquitin-protein ligase makorin-1 OS=Seriola quinqueradiata OX=8161...	[more]
Q4VBT5	1.1e-60	33.41	Probable E3 ubiquitin-protein ligase makorin-1 OS=Danio rerio OX=7955 GN=mkrn1 P...	[more]

BLAST of Carg13450 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H7L6	3.5e-240	99.26	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460818...	[more]
A0A6J1KQR4	6.6e-239	98.51	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111497821 P...	[more]
A0A6J1H659	7.3e-238	99.25	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460818...	[more]
A0A6J1H5V2	7.3e-238	99.25	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111460818...	[more]
A0A6J1KSE8	1.4e-236	98.50	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111497821 P...	[more]

BLAST of Carg13450 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G08505.2	2.7e-115	48.99	zinc finger (CCCH-type/C3HC4-type RING finger) family protein	[more]
AT3G08505.1	2.7e-115	48.99	zinc finger (CCCH-type/C3HC4-type RING finger) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 206..263 e-value: 1.7E-7 score: 40.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 206..264 score: 12.371254
IPR000571	Zinc finger, CCCH-type	SMART	SM00356	c3hfinal6	coord: 29..55 e-value: 0.0013 score: 28.0 coord: 293..321 e-value: 0.36 score: 19.8 coord: 1..27 e-value: 5.7E-5 score: 32.5 coord: 137..163 e-value: 0.0033 score: 26.7
IPR000571	Zinc finger, CCCH-type	PROSITE	PS50103	ZF_C3H1	coord: 1..28 score: 13.685178
IPR000571	Zinc finger, CCCH-type	PROSITE	PS50103	ZF_C3H1	coord: 293..322 score: 13.638232
IPR000571	Zinc finger, CCCH-type	PROSITE	PS50103	ZF_C3H1	coord: 29..56 score: 15.112321
IPR000571	Zinc finger, CCCH-type	PROSITE	PS50103	ZF_C3H1	coord: 136..164 score: 12.436428
None	No IPR available	GENE3D	1.20.120.1350		coord: 1..31 e-value: 1.9E-6 score: 29.4
None	No IPR available	GENE3D	3.30.1370.210		coord: 32..84 e-value: 1.5E-5 score: 27.0
None	No IPR available	PFAM	PF14608	zf-CCCH_2	coord: 141..161 e-value: 3.1 score: 8.5 coord: 298..319 e-value: 0.012 score: 16.2
None	No IPR available	PANTHER	PTHR11224:SF52	ZINC FINGER CCCH DOMAIN-CONTAINING PROTEIN 69-LIKE	coord: 407..448 coord: 1..324
None	No IPR available	CDD	cd16521	RING-HC_MKRN	coord: 206..264 e-value: 3.13568E-17 score: 73.505
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 194..270
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 206..263 e-value: 2.2E-7 score: 30.6
IPR041686	Zinc-finger CCCH domain	PFAM	PF15663	zf-CCCH_3	coord: 6..62 e-value: 2.3E-7 score: 31.0
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 177..269 e-value: 5.5E-10 score: 40.5
IPR045072	MKRN-like	PANTHER	PTHR11224	MAKORIN-RELATED	coord: 1..324
IPR045072	MKRN-like	PANTHER	PTHR11224	MAKORIN-RELATED	coord: 407..448
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 230..239
IPR036855	Zinc finger, CCCH-type superfamily	SUPERFAMILY	90229	CCCH zinc finger	coord: 32..53
IPR036855	Zinc finger, CCCH-type superfamily	SUPERFAMILY	90229	CCCH zinc finger	coord: 3..26

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg13450-RA	Carg13450-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0046872	metal ion binding