Carg12421 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12421
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPhosphomethylpyrimidine synthase
LocationCarg_Chr01: 3500200 .. 3504495 (+)
RNA-Seq ExpressionCarg12421
SyntenyCarg12421
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAATTTTCTCTCTTAATTTTGCATTTTCTTTCACCTGCTAATTATAAGGAAGTAATAGCAGAGTATCATTGACTTGATTATCATACAAGGCTATGGCCGCTCACTTATCATCGAAATGAACTTGAATTTAACCGGGAGCTACTCAACCAACTTACATAACACCGTAGTTAACGATAAAAATTTAGAAGGCTAACTGAGCTAGTTAAAATGTGAAGTAGCTAACAACAATTGGTTACTCACAACACCGCTCGAGTGTCAGATTGAGTTCTACTAAGAAAAGGCTTTTTGTTCTCTCTGTTTTTTTGGGTCTGATTTTGAAATTCATCGTTGTGCCTGATTCTGACAATCATTTATCAAGTTCAAGAAACTCTTTTCTTTTTCTTCGATGGTAGGATTTTGACCATTAATTTTTTATTTTCTAGATCAAAGATGTCGTCAATTCAAGCTACCATAACACCTGCTGTTGGAAAGAGTGGGAACCGTTCCTACTCTACGAAATTGCTTAAGTCTGCCTTCTTTCCTGGGTTTGATGTGGTTGGTCGTGTTTCCGGTGCATGCAAGGAATTACACCCTTCATCTATTACCTTAGCACCTAGAGCCACTTTAACCTTTGACCCCCCAGAAACCAGCACGGAGAAGGCCAAAAACAAGAAACATACAGTTGATCCTTCTTCTCCTGATTTTTTGCCGCTTCCTTCATTTGAGCTGTGTTTTCCAAAAAGCACAAAAGAACACAAGTGAGTGAATTCATTGGAGTATCGTCTTGTTTCATAGTTCTTGTAACTGTTGCAAAGCTAGATAGATGATTATCGTAAACTGATATCATATACATCTGCAATTATTGATGCATGTTCTGCTATGCAGGGAAATTGTTCACGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGACGGGTTCATTTATCTGGTGATGAACCACACTTTGACAATTATGACACTAGTGGTCCTCAAAACATTAGCCCCCGTGTTGGTAAGATGATCAACTTTTAACTGTTCCTTAAAGAGACTCATTTTTGCTATATTTTTTTTTGTAGCATTTATTGGTTTTCTTTATTATTTTTAATTGTTCGTGAATGTATACTTTTACTGAAAATATAATTTTTTGTGAAAATTGTTTGGACTCTGATGCTCAGATATACGTATGTTATTTTAAGTCTGCTGCAAAAGCAGACTATTTCTTGCCTAAACATTAAAACCAACTGCTGTGAAACTGACATCCTTTAGCAGTGGGCCTATCTGACTCTATAAGAAAACATAAATAGCGGCAAGTATGATTGCTTTCTATAATGGGCATTCCCTTTGAGCTTTTGTTAATTGTCTTGTAATCCAGTTTCTATAAATAATGCTTGCTTACTTGATTTCCATGATGAATCAATACAAACAGTTCTGGTAATTTTTTTTTCTTTGGTGTCCTGTGTGGGTGTATGTTACCTATCTCTTTCAATTTTTGACAATCAACAATGTTATATAGGATTGCCTAAACTTCGGAAGGACTGGGTTGATAGGAGAGAAAAATTAGGAGCACCAAGATGCACACAGATGTATTATGCCAAGCAAGGAATCATAACTGAGGAAATGTTGTTTTGTGCCACTCGTGAGAAGCTTGACCCAGAGTTTGTGAGGTCAGAGGTTGCTCGTGGGCGGGCAATCATCCCTTCCAACAAGAAGCATTTGGAATTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTTGCGAGTTCAATTGAAGAAGAAGTTTATAAAGTCCAATGGGCGACCATGTGGGGAGCTGATACTGTCATGGACCTCTCTACTGGTCGTCACATACATGAGACTCGTGAGTGGATTTTGCGTAACTCCCCAGTACCATTAGGAACTGTACCCATATATCAGGCACTTGAGAAAGTGAATGGAATAGCTGAAAATCTCACCTGGGATATTTTCAGAGAAACACTGATTGAACAAGCTGAGCAGGGTGTAGACTATTTCACTATTCATGCTGGGGTCTTACTGCGATACATCCCTCTAACAGCCAAAAGAATGACGGGAATCGTATCACGTGGAGGATCTATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAACTTTGCTTATGAACATTGGGATGACATACTTGACATCTGTAATCAGTATGATATATCCCTATCGATTGGTGATGGGCTGAGACCTGGTTCAATTTATGATGCTAATGACACTGCTCAATTTGCGGAGCTCTTAACTCAGGGTGAACTGACTCGTAGAGCATGGGAAAAAGATGTGCAGGTATGAATAAAAGTCTCTCTTGCATACATGCATCCACGTGCTAACAATCAAACTTACGTTAGTTATATGCATTTGTGAGGAAACCTATTACTCGCAGTTATATGCAGTGAAAGAACATCTTATTGTTGTAACTTTTGTAAGAACCACTCAAGCCGTACCTATTACAGCTTGTTTGGTTGAGAATGTACACTAAAAATATGGAACTAAAGGTTCCATTATTTTCAATAGCTAATACAGGCAATTATTTTCTGCTATTTTCATTTTAGAGATTAGGAATGTTATTTTTTACTTGATTCTAACTAGTGGCCAAATATTTGAGTTTCAGGTAATGAATGAAGGACCAGGACATGTTCCAATGCATAAGATCCCTGAAAATATGCAAAAACAGCTTGAGTGGTGTAATGAAGCACCTTTTTACACTCTTGGCCCCTTGACTACGGATGTTGCTCCTGGATATGACCACATTACCTCTGCCATTGGTGCAGCCAATATTGGGGCCCTTGGCACGGCTCTTCTCTGTTACGTGACACCGAAAGAGCACTTAGGATTGCCGAATCGAGATGACGTGAAGACTGGAGTAATATCATATAAGATAGCTGCTCATGCAGCTGATCTAGCCAAAGGTCACCCACATGCACAGTCATGGGATGATGCACTGAGTAAGGCAAGATTCGAATTTCGGTGGATGGATCAATTTGCTTTGTCAATGGACCCTATGACTGCCATGTCCTTTCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTAGCCCATTTCTGCTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACTGAAGATGTGCGGAAGTATGCCGAGGAACACGGGTACGGGAACGTAGAAGAAGCTCTGCAGCAAGGGATGGATGCTATGAGTGCTGAATTTTTGTCTGCAAAGAAAACCATTAGTGGTGAACAACATGGTGAAACTGGTGGGGAAATCTACTTGCCTGCAAGCTACGTGGACTCCATGAAAAGGTAAAGGTTAGGAATCACAATTTCTTTCTGGTCTTTTTGATTTTATTGCTTGGTTTTCTTAGCATAGAACAAGAACTTGAATAACCATGTATCAATGTCTTCATATTCCTCACTTGAACGGATTTGGTACAAAGCGTTCTCATATTGGATCTCTACACATACACTACCGAATCCCCTCGAGAACCTGCTCGATTATTATCTGACTTTGATTATCTTGTCTAGATTAAATATCCTTGTAGGCTTCATGGATATAATTAGTTAATGCTGCAGCCTGCAAGTTTATTTCTTGGGTCTTATGCATACAGTCCAATGGTAATAGTTAGTCAGTCACATCTTTAGATGAGGAAGAACCCTACTCAGTAAGGTAAGGAATAAGGTTTTGTAATCGGTACGATACCCCTTTTTCGATAAGGGGCAACGAGAACCATTGCTCTCAGTTTTTAGCGATTACTCCGTTAATTCCTGTGGTTGATTCTTACAAATGTACGAATTTGCAGGACCGCTAGGGAAGGAGAAAGGGTTTGAAGTGCAGGAGACTTGTTTGAGCGGACAGATCATTTATGCCTGCTGCTTGAGTTTCCTAATTCTGGCCATGGAAGAGTACGAAGTTTAGCTTGTATCTATTCCCCTTTTATTGGCTGTATTTAGTTTGTCTGCTTTGTGTCTCTCTATCTGTTGGAACTAAGAAAAAAGTTATTGCTGCTGTTCTTGATGTCTTGGCTGTTGTGTCTCTTTCATCATCTTTGTTTTGATTCCTATATTGATGTAGAAACGCACCGGGGGTGCCTCGACCAAGTTCTTAGCTTTGCTCGGCTGCGAGCTTAACTTGGTCAGGCTGAGATAGTCCCTTTGAACCTGAACAGGATAATGCCTGCGTAGGGAGCGTGCTTTTCTGTTTTTTGTTTGTGTTTGTATACAGGAAAAACGTCGCTACACTGCTCGTGGCTGTCGTTATGGTCGACCATCTATTTGA

mRNA sequence

AAAATTTTCTCTCTTAATTTTGCATTTTCTTTCACCTGCTAATTATAAGGAAGTAATAGCAGAGTATCATTGACTTGATTATCATACAAGGCTATGGCCGCTCACTTATCATCGAAATGAACTTGAATTTAACCGGGAGCTACTCAACCAACTTACATAACACCGTAGTTAACGATAAAAATTTAGAAGGCTAACTGAGCTAGTTAAAATGTGAAGTAGCTAACAACAATTGGTTACTCACAACACCGCTCGAGTGTCAGATTGAGTTCTACTAAGAAAAGGCTTTTTGTTCTCTCTGTTTTTTTGGGTCTGATTTTGAAATTCATCGTTGTGCCTGATTCTGACAATCATTTATCAAGTTCAAGAAACTCTTTTCTTTTTCTTCGATGGTAGGATTTTGACCATTAATTTTTTATTTTCTAGATCAAAGATGTCGTCAATTCAAGCTACCATAACACCTGCTGTTGGAAAGAGTGGGAACCGTTCCTACTCTACGAAATTGCTTAAGTCTGCCTTCTTTCCTGGGTTTGATGTGGTTGGTCGTGTTTCCGGTGCATGCAAGGAATTACACCCTTCATCTATTACCTTAGCACCTAGAGCCACTTTAACCTTTGACCCCCCAGAAACCAGCACGGAGAAGGCCAAAAACAAGAAACATACAGTTGATCCTTCTTCTCCTGATTTTTTGCCGCTTCCTTCATTTGAGCTGTGTTTTCCAAAAAGCACAAAAGAACACAAGGAAATTGTTCACGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGACGGGTTCATTTATCTGGTGATGAACCACACTTTGACAATTATGACACTAGTGGTCCTCAAAACATTAGCCCCCGTGTTGGATTGCCTAAACTTCGGAAGGACTGGGTTGATAGGAGAGAAAAATTAGGAGCACCAAGATGCACACAGATGTATTATGCCAAGCAAGGAATCATAACTGAGGAAATGTTGTTTTGTGCCACTCGTGAGAAGCTTGACCCAGAGTTTGTGAGGTCAGAGGTTGCTCGTGGGCGGGCAATCATCCCTTCCAACAAGAAGCATTTGGAATTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTTGCGAGTTCAATTGAAGAAGAAGTTTATAAAGTCCAATGGGCGACCATGTGGGGAGCTGATACTGTCATGGACCTCTCTACTGGTCGTCACATACATGAGACTCGTGAGTGGATTTTGCGTAACTCCCCAGTACCATTAGGAACTGTACCCATATATCAGGCACTTGAGAAAGTGAATGGAATAGCTGAAAATCTCACCTGGGATATTTTCAGAGAAACACTGATTGAACAAGCTGAGCAGGGTGTAGACTATTTCACTATTCATGCTGGGGTCTTACTGCGATACATCCCTCTAACAGCCAAAAGAATGACGGGAATCGTATCACGTGGAGGATCTATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAACTTTGCTTATGAACATTGGGATGACATACTTGACATCTGTAATCAGTATGATATATCCCTATCGATTGGTGATGGGCTGAGACCTGGTTCAATTTATGATGCTAATGACACTGCTCAATTTGCGGAGCTCTTAACTCAGGGTGAACTGACTCGTAGAGCATGGGAAAAAGATGTGCAGGTAATGAATGAAGGACCAGGACATGTTCCAATGCATAAGATCCCTGAAAATATGCAAAAACAGCTTGAGTGGTGTAATGAAGCACCTTTTTACACTCTTGGCCCCTTGACTACGGATGTTGCTCCTGGATATGACCACATTACCTCTGCCATTGGTGCAGCCAATATTGGGGCCCTTGGCACGGCTCTTCTCTGTTACGTGACACCGAAAGAGCACTTAGGATTGCCGAATCGAGATGACGTGAAGACTGGAGTAATATCATATAAGATAGCTGCTCATGCAGCTGATCTAGCCAAAGGTCACCCACATGCACAGTCATGGGATGATGCACTGAGTAAGGCAAGATTCGAATTTCGGTGGATGGATCAATTTGCTTTGTCAATGGACCCTATGACTGCCATGTCCTTTCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTAGCCCATTTCTGCTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACTGAAGATGTGCGGAAGTATGCCGAGGAACACGGGTACGGGAACGTAGAAGAAGCTCTGCAGCAAGGGATGGATGCTATGAGTGCTGAATTTTTGTCTGCAAAGAAAACCATTAGTGGTGAACAACATGGTGAAACTGGTGGGGAAATCTACTTGCCTGCAAGCTACGTGGACTCCATGAAAAGGACCGCTAGGGAAGGAGAAAGGAAACGCACCGGGGGTGCCTCGACCAAGTTCTTAGCTTTGCTCGGCTGCGAGCTTAACTTGGAAAAACGTCGCTACACTGCTCGTGGCTGTCGTTATGGTCGACCATCTATTTGA

Coding sequence (CDS)

ATGTCGTCAATTCAAGCTACCATAACACCTGCTGTTGGAAAGAGTGGGAACCGTTCCTACTCTACGAAATTGCTTAAGTCTGCCTTCTTTCCTGGGTTTGATGTGGTTGGTCGTGTTTCCGGTGCATGCAAGGAATTACACCCTTCATCTATTACCTTAGCACCTAGAGCCACTTTAACCTTTGACCCCCCAGAAACCAGCACGGAGAAGGCCAAAAACAAGAAACATACAGTTGATCCTTCTTCTCCTGATTTTTTGCCGCTTCCTTCATTTGAGCTGTGTTTTCCAAAAAGCACAAAAGAACACAAGGAAATTGTTCACGAAGAAACTGGGCATGTGCTCAAAGTACCCTTTCGACGGGTTCATTTATCTGGTGATGAACCACACTTTGACAATTATGACACTAGTGGTCCTCAAAACATTAGCCCCCGTGTTGGATTGCCTAAACTTCGGAAGGACTGGGTTGATAGGAGAGAAAAATTAGGAGCACCAAGATGCACACAGATGTATTATGCCAAGCAAGGAATCATAACTGAGGAAATGTTGTTTTGTGCCACTCGTGAGAAGCTTGACCCAGAGTTTGTGAGGTCAGAGGTTGCTCGTGGGCGGGCAATCATCCCTTCCAACAAGAAGCATTTGGAATTGGAGCCCATGATTGTGGGTAGAAATTTCTTGGTCAAAGTAAATGCAAATATTGGAAACTCTGCTGTTGCGAGTTCAATTGAAGAAGAAGTTTATAAAGTCCAATGGGCGACCATGTGGGGAGCTGATACTGTCATGGACCTCTCTACTGGTCGTCACATACATGAGACTCGTGAGTGGATTTTGCGTAACTCCCCAGTACCATTAGGAACTGTACCCATATATCAGGCACTTGAGAAAGTGAATGGAATAGCTGAAAATCTCACCTGGGATATTTTCAGAGAAACACTGATTGAACAAGCTGAGCAGGGTGTAGACTATTTCACTATTCATGCTGGGGTCTTACTGCGATACATCCCTCTAACAGCCAAAAGAATGACGGGAATCGTATCACGTGGAGGATCTATTCATGCAAAGTGGTGTTTGGCTCATCATAAGGAAAACTTTGCTTATGAACATTGGGATGACATACTTGACATCTGTAATCAGTATGATATATCCCTATCGATTGGTGATGGGCTGAGACCTGGTTCAATTTATGATGCTAATGACACTGCTCAATTTGCGGAGCTCTTAACTCAGGGTGAACTGACTCGTAGAGCATGGGAAAAAGATGTGCAGGTAATGAATGAAGGACCAGGACATGTTCCAATGCATAAGATCCCTGAAAATATGCAAAAACAGCTTGAGTGGTGTAATGAAGCACCTTTTTACACTCTTGGCCCCTTGACTACGGATGTTGCTCCTGGATATGACCACATTACCTCTGCCATTGGTGCAGCCAATATTGGGGCCCTTGGCACGGCTCTTCTCTGTTACGTGACACCGAAAGAGCACTTAGGATTGCCGAATCGAGATGACGTGAAGACTGGAGTAATATCATATAAGATAGCTGCTCATGCAGCTGATCTAGCCAAAGGTCACCCACATGCACAGTCATGGGATGATGCACTGAGTAAGGCAAGATTCGAATTTCGGTGGATGGATCAATTTGCTTTGTCAATGGACCCTATGACTGCCATGTCCTTTCATGATGAAACCTTGCCATCAGAAGGTGCCAAGGTAGCCCATTTCTGCTCCATGTGTGGACCCAAGTTCTGCTCCATGAAGATAACTGAAGATGTGCGGAAGTATGCCGAGGAACACGGGTACGGGAACGTAGAAGAAGCTCTGCAGCAAGGGATGGATGCTATGAGTGCTGAATTTTTGTCTGCAAAGAAAACCATTAGTGGTGAACAACATGGTGAAACTGGTGGGGAAATCTACTTGCCTGCAAGCTACGTGGACTCCATGAAAAGGACCGCTAGGGAAGGAGAAAGGAAACGCACCGGGGGTGCCTCGACCAAGTTCTTAGCTTTGCTCGGCTGCGAGCTTAACTTGGAAAAACGTCGCTACACTGCTCGTGGCTGTCGTTATGGTCGACCATCTATTTGA

Protein sequence

MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLTFDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRRVHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEEMLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAENLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFRWMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGNVEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGERKRTGGASTKFLALLGCELNLEKRRYTARGCRYGRPSI
Homology
[Close]
BLAST of Carg12421 vs. NCBI nr
Match: KAG6607299.1 (Phosphomethylpyrimidine synthase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036978.1 Phosphomethylpyrimidine synthase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 691/691 (100.00%), Postives = 691/691 (100.00%), Query Frame = 0 

Query: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60
           MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT
Sbjct: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60

Query: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120
           FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR
Sbjct: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180
           VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300

Query: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600

Query: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGERKRTGGA 660
           VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGERKRTGGA
Sbjct: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGERKRTGGA 660

Query: 661 STKFLALLGCELNLEKRRYTARGCRYGRPSI 692
           STKFLALLGCELNLEKRRYTARGCRYGRPSI
Sbjct: 661 STKFLALLGCELNLEKRRYTARGCRYGRPSI 691

[Close]
BLAST of Carg12421 vs. NCBI nr
Match: XP_022949305.1 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 [Cucurbita moschata] >XP_022949306.1 phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 [Cucurbita moschata] >XP_023525249.1 phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucurbita pepo subsp. pepo] >XP_023525250.1 phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucurbita pepo subsp. pepo] >XP_023525251.1 phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucurbita pepo subsp. pepo] >XP_023525252.1 phosphomethylpyrimidine synthase, chloroplastic isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1355.1 bits (3506), Expect = 0.0e+00
Identity = 654/654 (100.00%), Postives = 654/654 (100.00%), Query Frame = 0 

Query: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60
           MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT
Sbjct: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60

Query: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120
           FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR
Sbjct: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180
           VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300

Query: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600

Query: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 655
           VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER
Sbjct: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 654

[Close]
BLAST of Carg12421 vs. NCBI nr
Match: XP_023525246.1 (phosphomethylpyrimidine synthase, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1355.1 bits (3506), Expect = 0.0e+00
Identity = 654/654 (100.00%), Postives = 654/654 (100.00%), Query Frame = 0 

Query: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60
           MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT
Sbjct: 21  MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 80

Query: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120
           FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR
Sbjct: 81  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 140

Query: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180
           VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE
Sbjct: 141 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 200

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 201 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 260

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE
Sbjct: 261 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 320

Query: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 321 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 380

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 381 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 440

Query: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 441 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 500

Query: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 501 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 560

Query: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 561 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 620

Query: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 655
           VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER
Sbjct: 621 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 674

[Close]
BLAST of Carg12421 vs. NCBI nr
Match: XP_022949303.1 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1355.1 bits (3506), Expect = 0.0e+00
Identity = 654/654 (100.00%), Postives = 654/654 (100.00%), Query Frame = 0 

Query: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60
           MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT
Sbjct: 37  MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 96

Query: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120
           FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR
Sbjct: 97  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 156

Query: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180
           VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE
Sbjct: 157 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 216

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 217 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 276

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE
Sbjct: 277 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 336

Query: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 337 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 396

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 397 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 456

Query: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 457 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 516

Query: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 517 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 576

Query: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 577 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 636

Query: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 655
           VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER
Sbjct: 637 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 690

[Close]
BLAST of Carg12421 vs. NCBI nr
Match: XP_022998541.1 (phosphomethylpyrimidine synthase, chloroplastic isoform X1 [Cucurbita maxima])

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 653/654 (99.85%), Postives = 654/654 (100.00%), Query Frame = 0 

Query: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60
           MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT
Sbjct: 33  MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 92

Query: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120
           FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR
Sbjct: 93  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 152

Query: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180
           VHLSGDEP+FDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE
Sbjct: 153 VHLSGDEPNFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 212

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 213 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 272

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE
Sbjct: 273 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 332

Query: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 333 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 392

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 393 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 452

Query: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 453 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 512

Query: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 513 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 572

Query: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 573 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 632

Query: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 655
           VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER
Sbjct: 633 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 686

[Close]
BLAST of Carg12421 vs. ExPASy Swiss-Prot
Match: O82392 (Phosphomethylpyrimidine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=THIC PE=1 SV=1)

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 548/646 (84.83%), Postives = 591/646 (91.49%), Query Frame = 0 

Query: 2   SSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLTF 61
           +S+  T+   V  + N S   KL  S+  PGFDVV  V  A       + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  DPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRRV 121
           DPP T++E+AK +KHT+DPSSPDF P+PSFE CFPKSTKEHKE+VHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  +GL KLRK+W+DRREKLG PR TQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNS VP+GTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L W++FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVK GVI+YKIAAHAADLAK HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGNV 601
           MDQFALS+DPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG+ 
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]
BLAST of Carg12421 vs. ExPASy Swiss-Prot
Match: Q9KBJ4 (Phosphomethylpyrimidine synthase OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=thiC PE=3 SV=1)

HSP 1 Score: 730.3 bits (1884), Expect = 2.0e-209
Identity = 368/584 (63.01%), Postives = 431/584 (73.80%), Query Frame = 0 

Query: 90  SFELCFPKSTKEHKEIVHEETGHVLKVPFRRVH---LSGDEPHFDN-----YDTSGP--- 149
           S +  FP S K +      +    ++VPFR +       D    +N     YD+SGP   
Sbjct: 12  SIQSSFPNSQKRYVTGSRSD----IRVPFREISQHPTVTDSGEAENEPILVYDSSGPYTD 71

Query: 150 --QNISPRVGLPKLRKDWV----------------------------------------- 209
               ++   GLP +RK W+                                         
Sbjct: 72  ESYEVNIEKGLPAIRKSWILERGDCEKYEGRDVRPEDNGFTKDDDPRAAREVFPRTSSHK 131

Query: 210 DRREKLGAPRCTQMYYAKQGIITEEMLFCATREKLDPEFVRSEVARGRAIIPSNKKHLEL 269
             R K GA   TQM+YAK+GI+T EM F A RE + PEFVR E+ARGRAI+PSN  H E 
Sbjct: 132 PLRAKKGA-NVTQMHYAKKGIVTPEMEFVAIRENVSPEFVRDELARGRAILPSNINHPES 191

Query: 270 EPMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWI 329
           EPMI+GRNF VK+NANIGNSAV+SSI EEV K+ WAT WGADT+MDLSTG++IH TREWI
Sbjct: 192 EPMIIGRNFHVKINANIGNSAVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWI 251

Query: 330 LRNSPVPLGTVPIYQALEKVNGIAENLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPL 389
           +RNSPVP+GTVPIYQALEKVNG+AE+LTW+++R+TLIEQAEQGVDYFTIHAGVLLRYIPL
Sbjct: 252 IRNSPVPVGTVPIYQALEKVNGVAEDLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPL 311

Query: 390 TAKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYD 449
           TAKR+TGIVSRGGSI A+WCL HHKENF Y H+++I +I   YDI+ S+GDGLRPGSI D
Sbjct: 312 TAKRVTGIVSRGGSIMAQWCLYHHKENFLYTHFEEICEIMKTYDIAFSLGDGLRPGSIAD 371

Query: 450 ANDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLG 509
           AND AQFAEL T GELT+ AWE DVQVM EGPGHVPMH+I ENM KQL+ C EAPFYTLG
Sbjct: 372 ANDEAQFAELETLGELTKIAWEHDVQVMVEGPGHVPMHQIKENMDKQLDICQEAPFYTLG 431

Query: 510 PLTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHA 569
           PLTTD+APGYDHITSAIGAA IG  GTA+LCYVTPKEHLGLPN+DDV+ GVI+YKIAAHA
Sbjct: 432 PLTTDIAPGYDHITSAIGAAMIGWYGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHA 491

Query: 570 ADLAKGHPHAQSWDDALSKARFEFRWMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSM 620
           ADLAKGHP AQ  DDALSKARFEFRW DQF LS+DP  AM FHDETLP+EGAK AHFCSM
Sbjct: 492 ADLAKGHPGAQKRDDALSKARFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSM 551

[Close]
BLAST of Carg12421 vs. ExPASy Swiss-Prot
Match: Q5YNS3 (Phosphomethylpyrimidine synthase OS=Nocardia farcinica (strain IFM 10152) OX=247156 GN=thiC PE=3 SV=1)

HSP 1 Score: 723.8 bits (1867), Expect = 1.9e-207
Identity = 355/530 (66.98%), Postives = 420/530 (79.25%), Query Frame = 0 

Query: 114 LKVPFRRVHLSGDEPHFDNYDTSGP-----QNISPRVGLPKLRKDWVDRREKLGAPRCTQ 173
           L++P RR++L+  E  FD YDTSGP       I    GLPKLR  W   +  +  PR TQ
Sbjct: 32  LRIPVRRINLTNGET-FDVYDTSGPYTDENATIDLEAGLPKLRDSW--DKPTVAGPR-TQ 91

Query: 174 MYYAKQGIITEEMLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKV 233
           + +A+ GI+T EM F A RE ++PE VR+EVA GRA+IP+N  H ELEP I+G+ FLVK+
Sbjct: 92  LAWARAGIVTPEMRFIAAREGVEPELVRAEVAAGRAVIPANHNHPELEPTIIGKKFLVKI 151

Query: 234 NANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPI 293
           NANIGNSAV+SSI EEV K+ WAT WGADT+MDLSTG++IHETREWILRNSPVP+GTVPI
Sbjct: 152 NANIGNSAVSSSIAEEVEKMVWATRWGADTIMDLSTGKNIHETREWILRNSPVPVGTVPI 211

Query: 294 YQALEKVNGIAENLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGG 353
           YQALEKVNG    LTW+I+R+T+IEQAEQGVDY T+HAGVLLRY+PLTAKR+TGIVSRGG
Sbjct: 212 YQALEKVNGDPAALTWEIYRDTVIEQAEQGVDYMTVHAGVLLRYVPLTAKRVTGIVSRGG 271

Query: 354 SIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQ 413
           SI A WCLAHH+E+F Y H++++ +I  +YD++ S+GDGLRPGS+ DAND AQFAEL T 
Sbjct: 272 SIMAAWCLAHHRESFLYTHFEELCEILARYDVTFSLGDGLRPGSVADANDEAQFAELRTL 331

Query: 414 GELTRRAWEKDVQVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHI 473
           GELT+ A    VQVM EGPGHVPMHKI EN++ + E C EAPFYTLGPL TD+AP YDHI
Sbjct: 332 GELTKIAKSHGVQVMIEGPGHVPMHKIVENVRLEEELCEEAPFYTLGPLATDIAPAYDHI 391

Query: 474 TSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSW 533
           TSAIGAA I   GTA+LCYVTPKEHLGLPNRDDVKTGVI+YKIAAHAADLAKGHPHAQ  
Sbjct: 392 TSAIGAAIIAQAGTAMLCYVTPKEHLGLPNRDDVKTGVITYKIAAHAADLAKGHPHAQQR 451

Query: 534 DDALSKARFEFRWMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITED 593
           DDALSKARFEFRW DQFALS+DP TA  +HDET+P+E AK AHFCSMCGPKFCSM+I+ D
Sbjct: 452 DDALSKARFEFRWRDQFALSLDPDTAREYHDETMPAEPAKTAHFCSMCGPKFCSMRISAD 511

Query: 594 VRKYAEEHGYGNVEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLP 639
           VR+YAE +   +V  A++ GM A SAEF             E+GG++YLP
Sbjct: 512 VREYAEANNLTDV-AAIEAGMAAKSAEF------------AESGGKVYLP 544

[Close]
BLAST of Carg12421 vs. ExPASy Swiss-Prot
Match: A7GUZ5 (Phosphomethylpyrimidine synthase OS=Bacillus cytotoxicus (strain DSM 22905 / CIP 110041 / 391-98 / NVH 391-98) OX=315749 GN=thiC PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 7.1e-207
Identity = 363/564 (64.36%), Postives = 424/564 (75.18%), Query Frame = 0 

Query: 103 KEIVHEETGHVLKVPFRRVHLSGDEPHFDN-----YDTSGP-----QNISPRVGLPKLRK 162
           K++  E +   +KVP R + LS D     N     YDTSGP       +    G+P  R+
Sbjct: 20  KKVYVEGSREGMKVPMREIELS-DTNGVPNSPIRVYDTSGPYTDPEYKVELEKGIPTPRR 79

Query: 163 DWV----------------------------------DR---REKLGAPRCTQMYYAKQG 222
           +W+                                  DR   R K GA   TQM+YA++G
Sbjct: 80  NWIIERGDVEEYEGREIKPEDDGVKAASNHTPVFPQMDRKPLRAKKGA-NVTQMHYARKG 139

Query: 223 IITEEMLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNS 282
           IIT EM + A RE ++PEFVR E+A GRAI+P+N  H E EPMI+GRNF VKVNANIGNS
Sbjct: 140 IITSEMEYVAIREGVEPEFVRKEIAEGRAILPANINHPEAEPMIIGRNFHVKVNANIGNS 199

Query: 283 AVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKV 342
           AV+SSI EEV K+ WAT WGADT+MDLSTG++IH TREWI+RN+PVP+GTVPIYQALEKV
Sbjct: 200 AVSSSIAEEVEKMTWATRWGADTIMDLSTGKNIHTTREWIIRNAPVPVGTVPIYQALEKV 259

Query: 343 NGIAENLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWC 402
            GIAENLTW+++R+TLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSI A+WC
Sbjct: 260 QGIAENLTWEVYRDTLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIMAQWC 319

Query: 403 LAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRA 462
           L HHKENF Y H+++I +I  QYD+S S+GDGLRPGSI DAND AQFAEL T GELT+ A
Sbjct: 320 LYHHKENFLYTHFEEICEIMKQYDVSFSLGDGLRPGSIADANDEAQFAELETLGELTKIA 379

Query: 463 WEKDVQVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAA 522
           W+ DVQVM EGPGHVPMH I ENM+K+++ C  APFYTLGPLTTD+APGYDHITSAIGAA
Sbjct: 380 WKHDVQVMIEGPGHVPMHLIKENMEKEIDICQGAPFYTLGPLTTDIAPGYDHITSAIGAA 439

Query: 523 NIGALGTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKA 582
            IG  GTA+LCYVTPKEHLGLPN+DDV+ GVI+YKIAAHAADLAKGH  AQ  DDALSKA
Sbjct: 440 MIGWFGTAMLCYVTPKEHLGLPNKDDVREGVITYKIAAHAADLAKGHKTAQQRDDALSKA 499

Query: 583 RFEFRWMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEE 620
           RFEFRW DQF LS+DP  AM FHDETLP+EGAK AHFCSMCGPKFCSMKI+ D+R+YA+E
Sbjct: 500 RFEFRWRDQFNLSLDPERAMEFHDETLPAEGAKTAHFCSMCGPKFCSMKISHDIREYAKE 559

[Close]
BLAST of Carg12421 vs. ExPASy Swiss-Prot
Match: A6VD86 (Phosphomethylpyrimidine synthase OS=Pseudomonas aeruginosa (strain PA7) OX=381754 GN=thiC PE=3 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 7.1e-207
Identity = 364/580 (62.76%), Postives = 417/580 (71.90%), Query Frame = 0 

Query: 114 LKVPFRRVHLSGDEPHFDN--------YDTSGPQ-----NISPRVGLPKLRKDWVD---- 173
           ++VP R + L+     F          YDTSGP       I  R GLP +R  W+D    
Sbjct: 38  IRVPVREISLADTPTAFGGEKNPPVFVYDTSGPYTDPEVRIDLRKGLPDVRSRWIDERGD 97

Query: 174 --------------------------------RREKLGAPRCTQMYYAKQGIITEEMLFC 233
                                           RR K GA   +QM+YAK+GIIT EM + 
Sbjct: 98  TEILPGLTSEFGQARLADASLDALRFAHVRTPRRAKPGA-NVSQMHYAKKGIITPEMEYI 157

Query: 234 ATRE----------------------------KLDPEFVRSEVARGRAIIPSNKKHLELE 293
           A RE                            ++ PEFVR EVARGRAIIP+N  H ELE
Sbjct: 158 AIRENMKLQEARAAGLLDQQHPGHSFGANIPKEITPEFVREEVARGRAIIPANINHTELE 217

Query: 294 PMIVGRNFLVKVNANIGNSAVASSIEEEVYKVQWATMWGADTVMDLSTGRHIHETREWIL 353
           PMI+GRNFLVK+N NIGNSA+ SSIEEEV K+ W   WGADTVMDLSTG+HIHETREWIL
Sbjct: 218 PMIIGRNFLVKINGNIGNSALGSSIEEEVEKLTWGIRWGADTVMDLSTGKHIHETREWIL 277

Query: 354 RNSPVPLGTVPIYQALEKVNGIAENLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLT 413
           RNSPVP+GTVPIYQALEKVNG+AE+LTW+IFR+TLIEQAEQGVDYFTIHAGVLLRY+PLT
Sbjct: 278 RNSPVPIGTVPIYQALEKVNGVAEDLTWEIFRDTLIEQAEQGVDYFTIHAGVLLRYVPLT 337

Query: 414 AKRMTGIVSRGGSIHAKWCLAHHKENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDA 473
           AKR+TGIVSRGGSI AKWCLAHHKENF Y H+++I +I   YD+S S+GDGLRPGS+ DA
Sbjct: 338 AKRVTGIVSRGGSIMAKWCLAHHKENFLYTHFEEICEIMKAYDVSFSLGDGLRPGSVADA 397

Query: 474 NDTAQFAELLTQGELTRRAWEKDVQVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGP 533
           ND AQF EL T GELT+ AW+ DVQVM EGPGHVPM  I ENM KQLE C+EAPFYTLGP
Sbjct: 398 NDAAQFGELETLGELTKIAWKHDVQVMIEGPGHVPMQLIKENMDKQLECCDEAPFYTLGP 457

Query: 534 LTTDVAPGYDHITSAIGAANIGALGTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAA 593
           LTTD+APGYDHITS IGAA IG  G A+LCYVTPKEHLGLPN+DDVKTG+I+YKIAAHAA
Sbjct: 458 LTTDIAPGYDHITSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAA 517

Query: 594 DLAKGHPHAQSWDDALSKARFEFRWMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMC 617
           DLAKGHP AQ  D+ALSKARFEFRW DQF L +DP TA +FHDETLP + AKVAHFCSMC
Sbjct: 518 DLAKGHPGAQIRDNALSKARFEFRWEDQFNLGLDPDTARAFHDETLPKDSAKVAHFCSMC 577

[Close]
BLAST of Carg12421 vs. ExPASy TrEMBL
Match: A0A6J1GCE0 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111452695 PE=3 SV=1)

HSP 1 Score: 1355.1 bits (3506), Expect = 0.0e+00
Identity = 654/654 (100.00%), Postives = 654/654 (100.00%), Query Frame = 0 

Query: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60
           MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT
Sbjct: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60

Query: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120
           FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR
Sbjct: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180
           VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300

Query: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600

Query: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 655
           VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER
Sbjct: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 654

[Close]
BLAST of Carg12421 vs. ExPASy TrEMBL
Match: A0A6J1GBM3 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452695 PE=3 SV=1)

HSP 1 Score: 1355.1 bits (3506), Expect = 0.0e+00
Identity = 654/654 (100.00%), Postives = 654/654 (100.00%), Query Frame = 0 

Query: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60
           MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT
Sbjct: 37  MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 96

Query: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120
           FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR
Sbjct: 97  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 156

Query: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180
           VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE
Sbjct: 157 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 216

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 217 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 276

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE
Sbjct: 277 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 336

Query: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 337 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 396

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 397 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 456

Query: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 457 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 516

Query: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 517 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 576

Query: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 577 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 636

Query: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 655
           VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER
Sbjct: 637 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 690

[Close]
BLAST of Carg12421 vs. ExPASy TrEMBL
Match: A0A6J1KCT8 (phosphomethylpyrimidine synthase, chloroplastic isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111493145 PE=3 SV=1)

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 653/654 (99.85%), Postives = 654/654 (100.00%), Query Frame = 0 

Query: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60
           MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT
Sbjct: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60

Query: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120
           FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR
Sbjct: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120

Query: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180
           VHLSGDEP+FDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE
Sbjct: 121 VHLSGDEPNFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE
Sbjct: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300

Query: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420

Query: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480

Query: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540

Query: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600

Query: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 655
           VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER
Sbjct: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 654

[Close]
BLAST of Carg12421 vs. ExPASy TrEMBL
Match: A0A6J1KAG1 (phosphomethylpyrimidine synthase, chloroplastic isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493145 PE=3 SV=1)

HSP 1 Score: 1352.4 bits (3499), Expect = 0.0e+00
Identity = 653/654 (99.85%), Postives = 654/654 (100.00%), Query Frame = 0 

Query: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60
           MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT
Sbjct: 33  MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 92

Query: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120
           FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR
Sbjct: 93  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 152

Query: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180
           VHLSGDEP+FDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE
Sbjct: 153 VHLSGDEPNFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 212

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 213 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 272

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE
Sbjct: 273 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 332

Query: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 333 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 392

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 393 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 452

Query: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 453 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 512

Query: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 513 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 572

Query: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 573 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 632

Query: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 655
           VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER
Sbjct: 633 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKRTAREGER 686

[Close]
BLAST of Carg12421 vs. ExPASy TrEMBL
Match: A0A6J1GCF8 (phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452695 PE=3 SV=1)

HSP 1 Score: 1342.0 bits (3472), Expect = 0.0e+00
Identity = 647/647 (100.00%), Postives = 647/647 (100.00%), Query Frame = 0 

Query: 1   MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 60
           MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT
Sbjct: 37  MSSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLT 96

Query: 61  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 120
           FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR
Sbjct: 97  FDPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRR 156

Query: 121 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 180
           VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE
Sbjct: 157 VHLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEE 216

Query: 181 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 240
           MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS
Sbjct: 217 MLFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASS 276

Query: 241 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 300
           IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE
Sbjct: 277 IEEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAE 336

Query: 301 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 360
           NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK
Sbjct: 337 NLTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHK 396

Query: 361 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 420
           ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV
Sbjct: 397 ENFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDV 456

Query: 421 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 480
           QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL
Sbjct: 457 QVMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGAL 516

Query: 481 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 540
           GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR
Sbjct: 517 GTALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFR 576

Query: 541 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 600
           WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN
Sbjct: 577 WMDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGN 636

Query: 601 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 648
           VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR
Sbjct: 637 VEEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 683

[Close]
BLAST of Carg12421 vs. TAIR 10
Match: AT2G29630.2 (thiaminC )

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 548/646 (84.83%), Postives = 591/646 (91.49%), Query Frame = 0 

Query: 2   SSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLTF 61
           +S+  T+   V  + N S   KL  S+  PGFDVV  V  A       + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  DPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRRV 121
           DPP T++E+AK +KHT+DPSSPDF P+PSFE CFPKSTKEHKE+VHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  +GL KLRK+W+DRREKLG PR TQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNS VP+GTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L W++FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVK GVI+YKIAAHAADLAK HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGNV 601
           MDQFALS+DPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG+ 
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]
BLAST of Carg12421 vs. TAIR 10
Match: AT2G29630.1 (thiaminC )

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 548/646 (84.83%), Postives = 591/646 (91.49%), Query Frame = 0 

Query: 2   SSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLTF 61
           +S+  T+   V  + N S   KL  S+  PGFDVV  V  A       + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  DPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRRV 121
           DPP T++E+AK +KHT+DPSSPDF P+PSFE CFPKSTKEHKE+VHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  +GL KLRK+W+DRREKLG PR TQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNS VP+GTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L W++FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVK GVI+YKIAAHAADLAK HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGNV 601
           MDQFALS+DPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG+ 
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

[Close]
BLAST of Carg12421 vs. TAIR 10
Match: AT2G29630.3 (thiaminC )

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 548/646 (84.83%), Postives = 591/646 (91.49%), Query Frame = 0 

Query: 2   SSIQATITPAVGKSGNRSYSTKLLKSAFFPGFDVVGRVSGACKELHPSSITLAPRATLTF 61
           +S+  T+   V  + N S   KL  S+  PGFDVV  V  A       + T   RATLTF
Sbjct: 3   ASVHCTLMSVVCNNKNHSARPKLPNSSLLPGFDVV--VQAAATRFKKETTT--TRATLTF 62

Query: 62  DPPETSTEKAKNKKHTVDPSSPDFLPLPSFELCFPKSTKEHKEIVHEETGHVLKVPFRRV 121
           DPP T++E+AK +KHT+DPSSPDF P+PSFE CFPKSTKEHKE+VHEE+GHVLKVPFRRV
Sbjct: 63  DPPTTNSERAKQRKHTIDPSSPDFQPIPSFEECFPKSTKEHKEVVHEESGHVLKVPFRRV 122

Query: 122 HLSGDEPHFDNYDTSGPQNISPRVGLPKLRKDWVDRREKLGAPRCTQMYYAKQGIITEEM 181
           HLSG EP FDNYDTSGPQN++  +GL KLRK+W+DRREKLG PR TQMYYAKQGIITEEM
Sbjct: 123 HLSGGEPAFDNYDTSGPQNVNAHIGLAKLRKEWIDRREKLGTPRYTQMYYAKQGIITEEM 182

Query: 182 LFCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRNFLVKVNANIGNSAVASSI 241
           L+CATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGR FLVKVNANIGNSAVASSI
Sbjct: 183 LYCATREKLDPEFVRSEVARGRAIIPSNKKHLELEPMIVGRKFLVKVNANIGNSAVASSI 242

Query: 242 EEEVYKVQWATMWGADTVMDLSTGRHIHETREWILRNSPVPLGTVPIYQALEKVNGIAEN 301
           EEEVYKVQWATMWGADT+MDLSTGRHIHETREWILRNS VP+GTVPIYQALEKV+GIAEN
Sbjct: 243 EEEVYKVQWATMWGADTIMDLSTGRHIHETREWILRNSAVPVGTVPIYQALEKVDGIAEN 302

Query: 302 LTWDIFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRMTGIVSRGGSIHAKWCLAHHKE 361
           L W++FRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKR+TGIVSRGGSIHAKWCLA+HKE
Sbjct: 303 LNWEVFRETLIEQAEQGVDYFTIHAGVLLRYIPLTAKRLTGIVSRGGSIHAKWCLAYHKE 362

Query: 362 NFAYEHWDDILDICNQYDISLSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 421
           NFAYEHWDDILDICNQYD++LSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ
Sbjct: 363 NFAYEHWDDILDICNQYDVALSIGDGLRPGSIYDANDTAQFAELLTQGELTRRAWEKDVQ 422

Query: 422 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDVAPGYDHITSAIGAANIGALG 481
           VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTD+APGYDHITSAIGAANIGALG
Sbjct: 423 VMNEGPGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAIGAANIGALG 482

Query: 482 TALLCYVTPKEHLGLPNRDDVKTGVISYKIAAHAADLAKGHPHAQSWDDALSKARFEFRW 541
           TALLCYVTPKEHLGLPNRDDVK GVI+YKIAAHAADLAK HPHAQ+WDDALSKARFEFRW
Sbjct: 483 TALLCYVTPKEHLGLPNRDDVKAGVIAYKIAAHAADLAKQHPHAQAWDDALSKARFEFRW 542

Query: 542 MDQFALSMDPMTAMSFHDETLPSEGAKVAHFCSMCGPKFCSMKITEDVRKYAEEHGYGNV 601
           MDQFALS+DPMTAMSFHDETLP++GAKVAHFCSMCGPKFCSMKITED+RKYAEE+GYG+ 
Sbjct: 543 MDQFALSLDPMTAMSFHDETLPADGAKVAHFCSMCGPKFCSMKITEDIRKYAEENGYGSA 602

Query: 602 EEALQQGMDAMSAEFLSAKKTISGEQHGETGGEIYLPASYVDSMKR 648
           EEA++QGMDAMS EF  AKKTISGEQHGE GGEIYLP SYV + ++
Sbjct: 603 EEAIRQGMDAMSEEFNIAKKTISGEQHGEVGGEIYLPESYVKAAQK 644

The following BLAST results are available for this feature:
BLAST of Carg12421 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6607299.10.0e+00100.00Phosphomethylpyrimidine synthase, chloroplastic, partial [Cucurbita argyrosperma... [more]
XP_022949305.10.0e+00100.00phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 [Cucurbita mosch... [more]
XP_023525246.10.0e+00100.00phosphomethylpyrimidine synthase, chloroplastic isoform X1 [Cucurbita pepo subsp... [more]
XP_022949303.10.0e+00100.00phosphomethylpyrimidine synthase, chloroplastic-like isoform X1 [Cucurbita mosch... [more]
XP_022998541.10.0e+0099.85phosphomethylpyrimidine synthase, chloroplastic isoform X1 [Cucurbita maxima][more]
BLAST of Carg12421 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O823920.0e+0084.83Phosphomethylpyrimidine synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 ... [more]
Q9KBJ42.0e-20963.01Phosphomethylpyrimidine synthase OS=Bacillus halodurans (strain ATCC BAA-125 / D... [more]
Q5YNS31.9e-20766.98Phosphomethylpyrimidine synthase OS=Nocardia farcinica (strain IFM 10152) OX=247... [more]
A7GUZ57.1e-20764.36Phosphomethylpyrimidine synthase OS=Bacillus cytotoxicus (strain DSM 22905 / CIP... [more]
A6VD867.1e-20762.76Phosphomethylpyrimidine synthase OS=Pseudomonas aeruginosa (strain PA7) OX=38175... [more]
BLAST of Carg12421 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1GCE00.0e+00100.00phosphomethylpyrimidine synthase, chloroplastic-like isoform X3 OS=Cucurbita mos... [more]
A0A6J1GBM30.0e+00100.00phosphomethylpyrimidine synthase, chloroplastic-like isoform X1 OS=Cucurbita mos... [more]
A0A6J1KCT80.0e+0099.85phosphomethylpyrimidine synthase, chloroplastic isoform X3 OS=Cucurbita maxima O... [more]
A0A6J1KAG10.0e+0099.85phosphomethylpyrimidine synthase, chloroplastic isoform X1 OS=Cucurbita maxima O... [more]
A0A6J1GCF80.0e+00100.00phosphomethylpyrimidine synthase, chloroplastic-like isoform X2 OS=Cucurbita mos... [more]
BLAST of Carg12421 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G29630.20.0e+0084.83thiaminC [more]
AT2G29630.10.0e+0084.83thiaminC [more]
AT2G29630.30.0e+0084.83thiaminC [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002817Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/BPFAMPF01964ThiC_Rad_SAMcoord: 167..587
e-value: 6.5E-197
score: 654.4
IPR002817Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/BTIGRFAMTIGR00190TIGR00190coord: 167..590
e-value: 5.4E-204
score: 675.7
IPR002817Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/BSFLDSFLDF00407phosphomethylpyrimidine_synthcoord: 167..587
e-value: 0.0
score: 655.5
IPR002817Phosphomethylpyrimidine synthase ThiC/5-hydroxybenzimidazole synthase BzaA/BPANTHERPTHR30557THIAMINE BIOSYNTHESIS PROTEIN THICcoord: 1..648
IPR038521ThiC/Bza, core domainGENE3D3.20.20.540Radical SAM ThiC family, central domaincoord: 209..523
e-value: 3.0E-168
score: 560.4
NoneNo IPR availableGENE3D6.10.250.620coord: 527..559
e-value: 1.6E-17
score: 65.0
NoneNo IPR availableSFLDSFLDS00113Radical_SAM_Phosphomethylpyricoord: 165..623
e-value: 0.0
score: 854.4
NoneNo IPR availableSFLDSFLDG01114phosphomethylpyrimidine_synthcoord: 165..621
e-value: 0.0
score: 836.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 60..81
NoneNo IPR availablePANTHERPTHR30557:SF2SUBFAMILY NOT NAMEDcoord: 1..648
IPR037509Phosphomethylpyrimidine synthaseHAMAPMF_00089ThiCcoord: 166..592
score: 94.820496

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12421-RACarg12421-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009228 thiamine biosynthetic process
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity
molecular_function GO:0016830 carbon-carbon lyase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0051536 iron-sulfur cluster binding