Homology
BLAST of Carg12214 vs. NCBI nr
Match:
KAG7021867.1 (putative E3 ubiquitin-protein ligase LIN-1, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 3882.0 bits (10066), Expect = 0.0e+00
Identity = 1986/1986 (100.00%), Postives = 1986/1986 (100.00%), Query Frame = 0
Query: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE
Sbjct: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
Query: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP
Sbjct: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
Query: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC
Sbjct: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
Query: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK
Sbjct: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
Query: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG
Sbjct: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
Query: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK
Sbjct: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
Query: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY
Sbjct: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
Query: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES
Sbjct: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
Query: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH
Sbjct: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
Query: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK
Sbjct: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
Query: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG
Sbjct: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
Query: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA
Sbjct: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
Query: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH
Sbjct: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
Query: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR
Sbjct: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
Query: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL
Sbjct: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
Query: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS
Sbjct: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
Query: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC
Sbjct: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
Query: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE
Sbjct: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
Query: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV
Sbjct: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
Query: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG
Sbjct: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
Query: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV
Sbjct: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
Query: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK
Sbjct: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
Query: 1321 VVCMSLNEREDVLVIGTSDGRIQKLQSLGCIQEDGKCRNMIADKADLAPILGSFMEVSND 1380
VVCMSLNEREDVLVIGTSDGRIQKLQSLGCIQEDGKCRNMIADKADLAPILGSFMEVSND
Sbjct: 1321 VVCMSLNEREDVLVIGTSDGRIQKLQSLGCIQEDGKCRNMIADKADLAPILGSFMEVSND 1380
Query: 1381 EQFEIIMFLSELVKLNRRTFNEQILQDIKDGGECSTMHSLLVYLQTAPRDQCPVVAGFLL 1440
EQFEIIMFLSELVKLNRRTFNEQILQDIKDGGECSTMHSLLVYLQTAPRDQCPVVAGFLL
Sbjct: 1381 EQFEIIMFLSELVKLNRRTFNEQILQDIKDGGECSTMHSLLVYLQTAPRDQCPVVAGFLL 1440
Query: 1441 QLDILVEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLARYV 1500
QLDILVEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLARYV
Sbjct: 1441 QLDILVEPRKSSIYREEAMDVLLSCLGNSDFPTAQISAAETIMSLQGRFSTSGRPLARYV 1500
Query: 1501 LLERVGFAKGCMKPKRRDNNSSGPGDVELSIKAEERANDEWERKMAFVLASHDFGLLFEP 1560
LLERVGFAKGCMKPKRRDNNSSGPGDVELSIKAEERANDEWERKMAFVLASHDFGLLFEP
Sbjct: 1501 LLERVGFAKGCMKPKRRDNNSSGPGDVELSIKAEERANDEWERKMAFVLASHDFGLLFEP 1560
Query: 1561 LAKGLKSKYAALFSACFVSATWLSHMLKVLPDTGILDAARVCLLDHFVSIFITTTDIEEK 1620
LAKGLKSKYAALFSACFVSATWLSHMLKVLPDTGILDAARVCLLDHFVSIFITTTDIEEK
Sbjct: 1561 LAKGLKSKYAALFSACFVSATWLSHMLKVLPDTGILDAARVCLLDHFVSIFITTTDIEEK 1620
Query: 1621 ALALLGMNSFVHQPEGLQYLSSNTKDIMRGLKELRRSTALAFEMLKVLCEGEESSAELWS 1680
ALALLGMNSFVHQPEGLQYLSSNTKDIMRGLKELRRSTALAFEMLKVLCEGEESSAELWS
Sbjct: 1621 ALALLGMNSFVHQPEGLQYLSSNTKDIMRGLKELRRSTALAFEMLKVLCEGEESSAELWS 1680
Query: 1681 HQELFLVDCSRNGEVLSIAYFKDKIISGHSDGTIKVWSVGGTNLHPLQETQEHSKGVTSL 1740
HQELFLVDCSRNGEVLSIAYFKDKIISGHSDGTIKVWSVGGTNLHPLQETQEHSKGVTSL
Sbjct: 1681 HQELFLVDCSRNGEVLSIAYFKDKIISGHSDGTIKVWSVGGTNLHPLQETQEHSKGVTSL 1740
Query: 1741 AIIESEEKLYSGSLDKTIKVWSLGSDVIQCIQVHDVKDQVHNLVVSKTIACFIPHGAGIR 1800
AIIESEEKLYSGSLDKTIKVWSLGSDVIQCIQVHDVKDQVHNLVVSKTIACFIPHGAGIR
Sbjct: 1741 AIIESEEKLYSGSLDKTIKVWSLGSDVIQCIQVHDVKDQVHNLVVSKTIACFIPHGAGIR 1800
Query: 1801 VYSWGGESKLLNSNKHVKCLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKP 1860
VYSWGGESKLLNSNKHVKCLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKP
Sbjct: 1801 VYSWGGESKLLNSNKHVKCLNLVRGKLYCGCHDSSIQEVDLATGTVSYIHIGSRKLLGKP 1860
Query: 1861 NIVQSLQIYEEQLFSASTTLDGAAVKIWSMSNNSVIETLSTAMDIRTMAVSSDLTYLGGR 1920
NIVQSLQIYEEQLFSASTTLDGAAVKIWSMSNNSVIETLSTAMDIRTMAVSSDLTYLGGR
Sbjct: 1861 NIVQSLQIYEEQLFSASTTLDGAAVKIWSMSNNSVIETLSTAMDIRTMAVSSDLTYLGGR 1920
Query: 1921 GGVVEIWGRDKLNKIDTLQTGRICKVACMTLNEREDVLFIGTSDGRIQTEELAGAAMGKQ 1980
GGVVEIWGRDKLNKIDTLQTGRICKVACMTLNEREDVLFIGTSDGRIQTEELAGAAMGKQ
Sbjct: 1921 GGVVEIWGRDKLNKIDTLQTGRICKVACMTLNEREDVLFIGTSDGRIQTEELAGAAMGKQ 1980
Query: 1981 NCFQNE 1987
NCFQNE
Sbjct: 1981 NCFQNE 1986
BLAST of Carg12214 vs. NCBI nr
Match:
XP_022933804.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Cucurbita moschata])
HSP 1 Score: 2524.2 bits (6541), Expect = 0.0e+00
Identity = 1310/1343 (97.54%), Postives = 1314/1343 (97.84%), Query Frame = 0
Query: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGI+SLSDEIALKPLELAAEALE
Sbjct: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIVSLSDEIALKPLELAAEALE 60
Query: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP
Sbjct: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
Query: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTE SIGDESEVF C
Sbjct: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTEHSIGDESEVFHC 180
Query: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK
Sbjct: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
Query: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
NEKW EMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG
Sbjct: 241 NEKWNEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
Query: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK
Sbjct: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
Query: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY
Sbjct: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
Query: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES
Sbjct: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
Query: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDV+H
Sbjct: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVQH 540
Query: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK
Sbjct: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
Query: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
RGVVEAVLLIYQLGLSSQTLQEMDVVGSLL+VVKKKEEDVNKMRLSHKSAAVLLLRKILG
Sbjct: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLSVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
Query: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
SKEGSPTVVAVLAENAIESILGSLKAKQVE RIAAVGILLRCIQEDWKCRNIIADKADLA
Sbjct: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEVRIAAVGILLRCIQEDWKCRNIIADKADLA 720
Query: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH
Sbjct: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
Query: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR
Sbjct: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
Query: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL
Sbjct: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
Query: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS
Sbjct: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
Query: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
IFT+TTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC
Sbjct: 961 IFTMTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
Query: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE
Sbjct: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
Query: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQ HDVKEQIHNLVVSKSV
Sbjct: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQAHDVKEQIHNLVVSKSV 1140
Query: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
ACFIPHGVGIR VYSWAGESKLLNSSKHVKCLNLMHG
Sbjct: 1141 ACFIPHGVGIR------------------------VYSWAGESKLLNSSKHVKCLNLMHG 1200
Query: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV
Sbjct: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
Query: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK
Sbjct: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1319
Query: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1344
VVCMSLNEREDVLVIGTSDGRIQ
Sbjct: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1319
BLAST of Carg12214 vs. NCBI nr
Match:
XP_022933805.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Cucurbita moschata])
HSP 1 Score: 2517.7 bits (6524), Expect = 0.0e+00
Identity = 1309/1343 (97.47%), Postives = 1313/1343 (97.77%), Query Frame = 0
Query: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGI+SLSDEIALKPLELAAEALE
Sbjct: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIVSLSDEIALKPLELAAEALE 60
Query: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP
Sbjct: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
Query: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTE SIGDESEVF C
Sbjct: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTEHSIGDESEVFHC 180
Query: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK
Sbjct: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
Query: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
NEKW EMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG
Sbjct: 241 NEKWNEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
Query: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK
Sbjct: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
Query: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY
Sbjct: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
Query: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES
Sbjct: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
Query: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDV+H
Sbjct: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVQH 540
Query: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK
Sbjct: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
Query: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
RGVVEAVLLIYQLGLSSQTLQEMDVVGSLL+VVKKKEEDVNKMRLSHKSAAVLLLRKILG
Sbjct: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLSVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
Query: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
SKEGSPTVVAVLAENAIESILGSLKAKQVE RIAAVGILLRCIQEDWKCRNIIADKADLA
Sbjct: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEVRIAAVGILLRCIQEDWKCRNIIADKADLA 720
Query: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
LILSSFTKVSNDEQFEIIMFLSELVKLN RTSNEKILRNIKDGSKCSTMHSLLTYLQTAH
Sbjct: 721 LILSSFTKVSNDEQFEIIMFLSELVKLN-RTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
Query: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR
Sbjct: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
Query: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL
Sbjct: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
Query: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS
Sbjct: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
Query: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
IFT+TTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC
Sbjct: 961 IFTMTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
Query: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE
Sbjct: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
Query: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQ HDVKEQIHNLVVSKSV
Sbjct: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQAHDVKEQIHNLVVSKSV 1140
Query: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
ACFIPHGVGIR VYSWAGESKLLNSSKHVKCLNLMHG
Sbjct: 1141 ACFIPHGVGIR------------------------VYSWAGESKLLNSSKHVKCLNLMHG 1200
Query: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV
Sbjct: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
Query: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK
Sbjct: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1318
Query: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1344
VVCMSLNEREDVLVIGTSDGRIQ
Sbjct: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1318
BLAST of Carg12214 vs. NCBI nr
Match:
XP_023007456.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Cucurbita maxima])
HSP 1 Score: 2497.2 bits (6471), Expect = 0.0e+00
Identity = 1294/1343 (96.35%), Postives = 1308/1343 (97.39%), Query Frame = 0
Query: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
MVATG ITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE
Sbjct: 1 MVATGAITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
Query: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
NAIFTDNSSIKSSSLRCAEELLLS+PENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP
Sbjct: 61 NAIFTDNSSIKSSSLRCAEELLLSVPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
Query: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
SLARSEIAPILFEELFLRHFLPIIHWFH+QR +ILTFFPSNLINNTTE SIGDE EVFPC
Sbjct: 121 SLARSEIAPILFEELFLRHFLPIIHWFHQQRFQILTFFPSNLINNTTEHSIGDEPEVFPC 180
Query: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA+HFKRVLERKDNGGSIAIPMVKLLDK
Sbjct: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAEHFKRVLERKDNGGSIAIPMVKLLDK 240
Query: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
NEKWKEMEQIA+QKLRTEQLC PNGRYNPIWIEEEQTL+VD TNKSKSASPPCVRSQVFG
Sbjct: 241 NEKWKEMEQIAKQKLRTEQLCFPNGRYNPIWIEEEQTLEVDFTNKSKSASPPCVRSQVFG 300
Query: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK
Sbjct: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
Query: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY
Sbjct: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
Query: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES
Sbjct: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
Query: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEG+V+H
Sbjct: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGNVQH 540
Query: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK
Sbjct: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
Query: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
RGVVEAVLLIYQLGLSSQTLQEMD+VGSLLNVVKKKEEDVNKM LSHKSAAVLLLRKILG
Sbjct: 601 RGVVEAVLLIYQLGLSSQTLQEMDIVGSLLNVVKKKEEDVNKMHLSHKSAAVLLLRKILG 660
Query: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA
Sbjct: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
Query: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
LILSSFTKVSN EQFEIIMFLSELVKLNRRTSNEKILRNIKDGS+CSTMHSLLTYLQTAH
Sbjct: 721 LILSSFTKVSNGEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSECSTMHSLLTYLQTAH 780
Query: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
RDQCPVVAGLLLQLDILVEPLKMSIYREEAMD LISCLRDSDFPTAQISAAETIMSLQGR
Sbjct: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDVLISCLRDSDFPTAQISAAETIMSLQGR 840
Query: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
F+TSGQPLARYFLLERAGLTKNHRKPIG+ENISDALGEVELTREEDKATDEWERKMAFVL
Sbjct: 841 FSTSGQPLARYFLLERAGLTKNHRKPIGTENISDALGEVELTREEDKATDEWERKMAFVL 900
Query: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS
Sbjct: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
Query: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
IFT+TTDIE KALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC
Sbjct: 961 IFTMTTDIEEKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
Query: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSV+GTNLHLLQE
Sbjct: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVKGTNLHLLQE 1080
Query: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV
Sbjct: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
Query: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
ACFIPHGVGIR VYSWAGESKLLNSSKHVKCLNLMHG
Sbjct: 1141 ACFIPHGVGIR------------------------VYSWAGESKLLNSSKHVKCLNLMHG 1200
Query: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV
Sbjct: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
Query: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKID LQIGRNCK
Sbjct: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDALQIGRNCK 1319
Query: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1344
VVCMSLNEREDVLVIGTSDGRIQ
Sbjct: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1319
BLAST of Carg12214 vs. NCBI nr
Match:
XP_023007466.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Cucurbita maxima])
HSP 1 Score: 2490.7 bits (6454), Expect = 0.0e+00
Identity = 1293/1343 (96.28%), Postives = 1307/1343 (97.32%), Query Frame = 0
Query: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
MVATG ITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE
Sbjct: 1 MVATGAITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
Query: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
NAIFTDNSSIKSSSLRCAEELLLS+PENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP
Sbjct: 61 NAIFTDNSSIKSSSLRCAEELLLSVPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
Query: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
SLARSEIAPILFEELFLRHFLPIIHWFH+QR +ILTFFPSNLINNTTE SIGDE EVFPC
Sbjct: 121 SLARSEIAPILFEELFLRHFLPIIHWFHQQRFQILTFFPSNLINNTTEHSIGDEPEVFPC 180
Query: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA+HFKRVLERKDNGGSIAIPMVKLLDK
Sbjct: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAEHFKRVLERKDNGGSIAIPMVKLLDK 240
Query: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
NEKWKEMEQIA+QKLRTEQLC PNGRYNPIWIEEEQTL+VD TNKSKSASPPCVRSQVFG
Sbjct: 241 NEKWKEMEQIAKQKLRTEQLCFPNGRYNPIWIEEEQTLEVDFTNKSKSASPPCVRSQVFG 300
Query: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK
Sbjct: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
Query: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY
Sbjct: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
Query: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES
Sbjct: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
Query: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEG+V+H
Sbjct: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGNVQH 540
Query: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK
Sbjct: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
Query: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
RGVVEAVLLIYQLGLSSQTLQEMD+VGSLLNVVKKKEEDVNKM LSHKSAAVLLLRKILG
Sbjct: 601 RGVVEAVLLIYQLGLSSQTLQEMDIVGSLLNVVKKKEEDVNKMHLSHKSAAVLLLRKILG 660
Query: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA
Sbjct: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
Query: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
LILSSFTKVSN EQFEIIMFLSELVKLN RTSNEKILRNIKDGS+CSTMHSLLTYLQTAH
Sbjct: 721 LILSSFTKVSNGEQFEIIMFLSELVKLN-RTSNEKILRNIKDGSECSTMHSLLTYLQTAH 780
Query: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
RDQCPVVAGLLLQLDILVEPLKMSIYREEAMD LISCLRDSDFPTAQISAAETIMSLQGR
Sbjct: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDVLISCLRDSDFPTAQISAAETIMSLQGR 840
Query: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
F+TSGQPLARYFLLERAGLTKNHRKPIG+ENISDALGEVELTREEDKATDEWERKMAFVL
Sbjct: 841 FSTSGQPLARYFLLERAGLTKNHRKPIGTENISDALGEVELTREEDKATDEWERKMAFVL 900
Query: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS
Sbjct: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
Query: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
IFT+TTDIE KALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC
Sbjct: 961 IFTMTTDIEEKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
Query: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSV+GTNLHLLQE
Sbjct: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVKGTNLHLLQE 1080
Query: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV
Sbjct: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
Query: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
ACFIPHGVGIR VYSWAGESKLLNSSKHVKCLNLMHG
Sbjct: 1141 ACFIPHGVGIR------------------------VYSWAGESKLLNSSKHVKCLNLMHG 1200
Query: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV
Sbjct: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
Query: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKID LQIGRNCK
Sbjct: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDALQIGRNCK 1318
Query: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1344
VVCMSLNEREDVLVIGTSDGRIQ
Sbjct: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1318
BLAST of Carg12214 vs. ExPASy Swiss-Prot
Match:
D1FP53 (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN PE=2 SV=1)
HSP 1 Score: 696.4 bits (1796), Expect = 9.2e-199
Identity = 488/1458 (33.47%), Postives = 759/1458 (52.06%), Query Frame = 0
Query: 41 ISLSDEIALKPLELAAEALENAIFTDNSSIKSSSLRCAEELL------------LSLPEN 100
+ SD+ L L+ EALE AI T N K + L AE++L +P +
Sbjct: 60 VEYSDQAVLANLDWGIEALEEAINTYNMETKLARLDYAEKMLQVCAMLNPKQKTAGVPNS 119
Query: 101 ACSSFL---LSLIYGLNHQNLNSALNLLHLFLSDPSLARSEIAPILFEELFLRHFLPIIH 160
S++ LS ++ L + + + L +F+ DP +R + AP L++ LFL H I+
Sbjct: 120 YLSAWAHLNLSYLWKLRNNIKSCIYHSLEMFIVDPFFSRIDFAPELWKNLFLPHMSSIVG 179
Query: 161 WFHEQRSEILTFFPSNLINNTTERSIGDESEVFPCTKSLSKLSIDQTLKLKELESNYERV 220
W+ E+R +++ L +T D +VF + S + +Q KL++LE Y
Sbjct: 180 WYSEERHKLMM---EVLPESTDFSYTADFDKVFNESLVFS-MRPNQLEKLQKLEQLYGES 239
Query: 221 LDKNCRVFAKHFKRVLERKDNGGSIAIPMV------------------------KLLDKN 280
LD+N R++AK++ + +PM+ +L K+
Sbjct: 240 LDENTRLYAKYYNDCMNPDSTSSKKVVPMLPIAEPPMTPLHELSRSVPDFVKFGPILPKS 299
Query: 281 EKWKEMEQIAEQKLR--TEQLCLPN-----GRYNPIWIEEE---QTLQVDCTNKSKSASP 340
+ + + L T + N G + +W +E + ++ D ++ AS
Sbjct: 300 SGFSMTTRRSNDGLNETTRENIASNSNHSKGEQSSLWAAKESIIEEIEDDLDSEHYDASV 359
Query: 341 PCVRSQVFGP---FNLISE----------QESSSSSRTFSIFKSTAQDQDDSS------- 400
+ +F P N+ E Q++ +S S +S + + SS
Sbjct: 360 DSDKINIFSPEPKKNIKDEDVEPKVYRSNQKNQMNSPNISPMESPRRASNYSSTNPLRRK 419
Query: 401 ------------------EIFSLGSETEFEENDH----EKTLFDSGQPKPKNQKLKQTIT 460
SL S + +DH ++ + K KN ++
Sbjct: 420 KESKFLRLLSNRFTGSIVSDHSLSSSPD-TSSDHIFTGDEEVMVRNNIKRKNDSQTPSMN 479
Query: 461 QSLELDYAMENSRSASPESGEKHIT-------------PPKDFVCPITCNIFYDPVTIET 520
Q E + +S E G + + PPKDFVCPIT IF DPVT+ET
Sbjct: 480 QDNENSLVLNDSSHCESEDGYQSSSSFPKLEKLTIGSKPPKDFVCPITGQIFSDPVTLET 539
Query: 521 GQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFV------ 580
GQTYER AIQEWL GN TCPIT Q L LPKTNYVLKRLI SW E+NP
Sbjct: 540 GQTYERKAIQEWLGTGNTTCPITRQALSANILPKTNYVLKRLIVSWKEQNPELAQEFSNS 599
Query: 581 --------------------LDKALDEAES----------------VAVLTSPVSFISQA 640
+ + D V+V SP S +SQA
Sbjct: 600 NTPRGSSCSPSAKDITMVSSIQRTTDSPSQKYKDDYIRQRNNRFTRVSVGASPTSVLSQA 659
Query: 641 SIDRSM-EMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRHMLLKPSVINGLVE 700
+++ + + IT+LCTSE L++ E AVL + R W + + + L KP+V++GLVE
Sbjct: 660 AVETIINSLTPYITSLCTSENLQDCEQAVLEIARLWKDSKTDPQIHSYLSKPTVVSGLVE 719
Query: 701 ILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFKRGVVEAVLLIYQL 760
IL S++ +VLR +I++LSEL F + V ETL V+SD +C+ L K G+ EA LLIYQL
Sbjct: 720 ILSASLNREVLRRSIYILSELIFSDERVGETLNSVDSDFDCLAMLLKNGLAEAALLIYQL 779
Query: 761 GLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLS--HKSAAVLLLRKIL-GSKEGSPTVVA 820
L E +++ SL+ V++ K ED++ +L+ K+AA+ +L +IL G E + +V A
Sbjct: 780 RPVFAQLSEHELIPSLIQVIQNKSEDIDDFQLAIDPKAAAIAILEQILIGGDEYNRSVNA 839
Query: 821 --VLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLALILSSFTK 880
V++ N I +I+ L + E R + ILL C+Q + C++ IA++ +L+ +L F
Sbjct: 840 SSVISANGIPAIVKYL--DKTEGRRPVISILLCCMQAEKSCKSSIANRIELSPVLELFHA 899
Query: 881 VSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAHRDQCPVVA 940
++ + + FLSELV+LNRRTS+ + L+ IKD STMH+ L YLQ A + VA
Sbjct: 900 GNDSVRGICVEFLSELVRLNRRTSSNQTLQIIKDEGAFSTMHTFLVYLQMAPMEHQIAVA 959
Query: 941 GLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGRFNTSGQPL 1000
LLLQLD+L EP KMSIYREEA++ LI L DF Q+ A + ++ L G +SG+
Sbjct: 960 SLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNNQMKALDALLFLIGHVTSSGKSY 1019
Query: 1001 ARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDK-ATDEWERKMAFVLVNHDFGL 1060
LL+ AG + + + +E + + + T E++K A W++++A VL NH+ G
Sbjct: 1020 TEAGLLKIAGFDQPYNVLMKAEQLGHSDNDFMETMEDEKNAMKSWQKRVASVLCNHENGS 1079
Query: 1061 LLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLSIFTVTTD 1120
+ + L + LKS + +C V ATWL+HML TLPDTG+ + AR LL+ +++ + +
Sbjct: 1080 IFQALEECLKSNSLKMAKSCLVLATWLTHMLFTLPDTGVRDVARKSLLEALMNVLQSSKN 1139
Query: 1121 IEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLCE-GQDSS 1180
+E K LA LA+ SF+ +P + L K+I R L++ ++ + +A D+LK L
Sbjct: 1140 LEEKILASLALKSFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADILKALLNLNSVDV 1199
Query: 1181 TELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQEIQEHSK 1240
TELWS E+ ++D S NGEVLS+ + +++SGH+DGT+KVW R ++QE +EH K
Sbjct: 1200 TELWSCKEVVELDLSSNGEVLSLHYLNGQVLSGHADGTIKVWDARKRIPRVIQETREHKK 1259
Query: 1241 GVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSVACFIPH 1300
VTSL S +KLYS SLDKTI+VW++ D ++CI V+DVKE ++ L + +AC++
Sbjct: 1260 AVTSL--CSSVDKLYSSSLDKTIRVWTIKPDGIKCIDVYDVKEAVYELAANAKLACYVTQ 1319
Query: 1301 GVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHGKLYCGC 1344
G G++ V++W K +N +K+VKCL + KLYCGC
Sbjct: 1320 GTGVK------------------------VFNWLDAPKFINFNKYVKCLAVSGDKLYCGC 1379
BLAST of Carg12214 vs. ExPASy Swiss-Prot
Match:
C6L7U1 (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBERUS PE=2 SV=2)
HSP 1 Score: 679.1 bits (1751), Expect = 1.5e-193
Identity = 396/999 (39.64%), Postives = 597/999 (59.76%), Query Frame = 0
Query: 395 PPKDFVCPITCNIFYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYV 454
PPKDFVCPIT IF DPVT+ETGQTYER AIQEWL GN TCPIT Q L + LPKTNYV
Sbjct: 511 PPKDFVCPITGQIFCDPVTLETGQTYERKAIQEWLRTGNTTCPITRQPLSASILPKTNYV 570
Query: 455 LKRLIASWLEENPNFVLD----------------------------------KALDEAES 514
LKRLI SW E+NP + K D A
Sbjct: 571 LKRLITSWKEQNPELAQEFSNVNTPRGSSCSPSAKDIPMLSTRQRTTDSPNHKNKDYARQ 630
Query: 515 -------VAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVERFWLEE 574
A+ TSP S +SQA+++ + ++ I++LCTSE L E E AVL + R +
Sbjct: 631 RSNRFMPAAITTSPTSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDS 690
Query: 575 NVEGDVRHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDV 634
+ L KP++INGLVEIL S + +VLR +I++LSEL F D V ETL V+SD
Sbjct: 691 KTNPQIHSYLSKPTIINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDF 750
Query: 635 NCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRL--SHKSA 694
+C+ TL K G+ EA LLIYQL L +++ SL++V++ K E+++ +L K A
Sbjct: 751 DCLATLLKNGLAEAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDA 810
Query: 695 AVLLLRK-ILGSKEGSPTVVA--VLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDW 754
A+ +L + ++G E S ++ A V++ N I +++ L +++E R + V +LL C+Q +
Sbjct: 811 AIAILEQTLMGGDEYSRSLNASSVISANGIPTLVKYL--ERMEGRRSVVSVLLCCMQAEK 870
Query: 755 KCRNIIADKADLALILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCS 814
C+N+IA++ +L+ +L F ++ + + FLSELV+LNRRTS +IL IKD S
Sbjct: 871 SCKNLIANRIELSPVLELFHSGNDSVRGTCVEFLSELVQLNRRTSCNQILHTIKDEGAFS 930
Query: 815 TMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQ 874
TMH+ L YLQ A + VA LLLQLD+L EP KMSIYREEA++ LI L DF Q
Sbjct: 931 TMHTFLVYLQMAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNTQ 990
Query: 875 ISAAETIMSLQGRFNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDK 934
+ A + ++ L G ++SG+ +LL+ AG + + + E + ++ T E++K
Sbjct: 991 MKALDALLFLIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEK 1050
Query: 935 -ATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGI 994
A + W++++A VL NH+ G + + L + LKS + +C V ATWL+HML TLPDTG+
Sbjct: 1051 NALNSWQKRIASVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTHMLYTLPDTGV 1110
Query: 995 LEAARIFLLDHFLSIFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFR 1054
+ AR LL+ +++ + ++E K LA LA+ +F+ +P + L K+I R L+ +
Sbjct: 1111 RDVARKSLLEEVINVLQSSKNLEEKILATLALKTFISDPSTHEALRVYAKSIYRTLRRLK 1170
Query: 1055 RSTPLAFDMLKVLCEGQD-SSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTM 1114
+ + +A D++KV+ + TELWS E+ ++D S NGEVLS+ + +++SGH+DGT+
Sbjct: 1171 KYSVVAVDIMKVILNLKSVDVTELWSCKEVVELDLSSNGEVLSMVYLNGQVLSGHTDGTI 1230
Query: 1115 KVWSVRGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVH 1174
KVW R ++QE EH+K VTSL S ++LYSGSLDKTI+VW++ SD ++CI V+
Sbjct: 1231 KVWDARKRIPRVIQETHEHTKAVTSL--CSSGDRLYSGSLDKTIRVWTIKSDGIKCIDVY 1290
Query: 1175 DVKEQIHNLVVSKSVACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKL 1234
D+KE +H L + +AC++ G G++ V++W+ KL
Sbjct: 1291 DIKEAVHELAANDKLACYVSQGTGVK------------------------VFNWSEAPKL 1350
Query: 1235 LNSSKHVKCLNLMHGKLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYD 1294
+N SK+VK L + KLYCGC SIQEVDL+T T + +G+RKLLGK IH+LQ++D
Sbjct: 1351 INFSKYVKSLAVAGDKLYCGCSGYSIQEVDLSTYTSNSFFTGTRKLLGK-QTIHSLQIHD 1410
Query: 1295 EQLFSASTALDGAAVKIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSRE 1344
+ LF+ +++D A KI S V+GSLST +D+ +A+ S+ + G K G +E+W ++
Sbjct: 1411 DYLFACGSSVDATAGKIFSLSQKMVVGSLSTGLDIHRIAINSDFIFAGTKFGTIEVWLKD 1470
BLAST of Carg12214 vs. ExPASy Swiss-Prot
Match:
D1FP57 (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE=1 SV=1)
HSP 1 Score: 654.1 bits (1686), Expect = 5.2e-186
Identity = 398/1049 (37.94%), Postives = 599/1049 (57.10%), Query Frame = 0
Query: 358 KPKNQKLKQTITQSLELDYAMENSRSASPESGEKHIT-------------PPKDFVCPIT 417
K KN ++ Q E + +S E G + + PPKDFVCPIT
Sbjct: 461 KRKNDSQTPSMNQDNENSLVLNDSSHCESEDGYQSSSSLPKLEKLSMGSKPPKDFVCPIT 520
Query: 418 CNIFYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLE 477
IF DPVT+ETGQTYER AIQEWL GN TCPIT Q L + LPKTNYVLKRLI SW E
Sbjct: 521 GQIFCDPVTLETGQTYERKAIQEWLRTGNTTCPITRQPLSASILPKTNYVLKRLITSWKE 580
Query: 478 ENPNFVLD----------------------------------KALDEAES-------VAV 537
+NP + K D A A+
Sbjct: 581 QNPELAQEFSNVNTPRGSSCSPSAKDIPMLSTRQRTTDSPNHKNKDYARQRSNRFMPAAI 640
Query: 538 LTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRHML 597
TSP S +SQA+++ + ++ I++LCTSE L E E AVL + R + + L
Sbjct: 641 TTSPTSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYL 700
Query: 598 LKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFKRG 657
KP++INGLVEIL S + +VLR +I++LSEL F D V ETL V+SD +C+ TL K G
Sbjct: 701 SKPTIINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG 760
Query: 658 VVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRL--SHKSAAVLLLRK-IL 717
+ EA LLIYQL L +++ SL++V++ K E+++ +L K AA+ +L + ++
Sbjct: 761 LAEAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLM 820
Query: 718 GSKEGSPTVVA--VLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKA 777
G E S ++ A V++ N I +++ L +++E R + V +LL C+Q + C+N+IA++
Sbjct: 821 GGDEYSRSLNASSVISANGIPTLVKYL--ERMEGRRSVVSVLLCCMQAEKSCKNLIANRI 880
Query: 778 DLALILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQ 837
+L+ +L F ++ + + FLSELV+LNRRTS ++L IKD STMH+ L YLQ
Sbjct: 881 ELSPVLELFHSGNDSVRGTCVEFLSELVQLNRRTSCNQLLHTIKDEGAFSTMHTFLVYLQ 940
Query: 838 TAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSL 897
A + VA LLLQLD+L EP KMSIYREEA++ LI L DF Q+ A + ++ L
Sbjct: 941 MAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNTQMKALDALLFL 1000
Query: 898 QGRFNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDK-ATDEWERKM 957
G ++SG+ +LL+ AG + + + E + ++ T E++K A + W++++
Sbjct: 1001 IGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKRI 1060
Query: 958 AFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLD 1017
A VL NH+ G + + L + LKS + +C V ATWL+ ML TLPDTG+ + AR LL+
Sbjct: 1061 ASVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTRMLYTLPDTGVRDVARKSLLE 1120
Query: 1018 HFLSIFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDML 1077
+ + + +E L L++ F+ +P + L K+I R L++ ++ + +A D+L
Sbjct: 1121 EVIKVLHSSKSLEDMILVTLSLYPFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADIL 1180
Query: 1078 KVLCE-GQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNL 1137
K L TELWS E+ ++D S NGEVLS+ + +++SG DGT KV R
Sbjct: 1181 KALLNLNSVDVTELWSCKEVVELDLSSNGEVLSLHYLNGQVLSGLMDGTSKVCDARKRIP 1240
Query: 1138 HLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLV 1197
++QE EH+K VTSL S ++LYS SLDKTI+VW++ SD ++CI V+D+KE +H L
Sbjct: 1241 RVIQETHEHTKAVTSL--CSSGDRLYSASLDKTIRVWTIKSDGIKCIDVYDIKEAVHELA 1300
Query: 1198 VSKSVACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCL 1257
+ +AC++ G G++ V++W+ KL+N SK+VK L
Sbjct: 1301 ANDKLACYVSQGTGVK------------------------VFNWSEAPKLINFSKYVKSL 1360
Query: 1258 NLMHGKLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTAL 1317
+ KLYCGC SIQEVDL+T T + +G+RKLLGK IH+LQ++D+ LF+ +++
Sbjct: 1361 AVAGDKLYCGCSGYSIQEVDLSTYTSNSFFTGTRKLLGK-QTIHSLQIHDDYLFACVSSV 1420
Query: 1318 DGAAVKIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQI 1344
D A KI S V+GSLST +D+ +A+ S+ + G K G +E+W ++K ++ ++Q+
Sbjct: 1421 DATAGKIFSLSQKMVVGSLSTGLDIHRIAINSDFIFAGTKFGTIEVWLKDKFTRVASIQM 1480
BLAST of Carg12214 vs. ExPASy Swiss-Prot
Match:
Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)
HSP 1 Score: 101.3 bits (251), Expect = 1.3e-19
Identity = 107/439 (24.37%), Postives = 197/439 (44.87%), Query Frame = 0
Query: 307 EQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPKNQKLKQ 366
++ S +S FS S ++D S + + +E + E+ E F + K + L++
Sbjct: 145 QRYGSLNSNKFSSALSEPMERDGFSNVIKIKAEEKL-ESVSETLHFGEEEEKQSSPPLRR 204
Query: 367 TITQSL-----------ELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIE 426
+ + SL LD + + S +S + +T P DF+CP++ + DPV +
Sbjct: 205 SSSISLAYYLSKDADTDRLDKMVNKNTDESKKSDK--LTIPVDFLCPVSLELMKDPVIVA 264
Query: 427 TGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEEN----PNFVL 486
TGQTYER+ IQ W+D GNLTCP T QKL+N L NYVL+ LI+ W E+ P +
Sbjct: 265 TGQTYERAYIQRWIDCGNLTCPKTQQKLENFTL-TPNYVLRSLISRWCAEHNIEQPAGYI 324
Query: 487 DKALDEAESVAVLTSPVSFISQASID---------RSMEMRR--------------AITN 546
+ + ++V+ + V +S S + RS+ R + N
Sbjct: 325 NGRTKNSGDMSVIRALVQRLSSRSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVN 384
Query: 547 LCTSEVLEEAETAVLCVERFWLEENVEGDVRHMLLKPSVINGLVEIL-VNSVDLQVLRAA 606
L TSE + E A+ CV + EN + +++ + +V++L +++ + AA
Sbjct: 385 LLTSEDVATQENAITCVLNLSIYEN----NKELIMFAGAVTSIVQVLRAGTMEARENAAA 444
Query: 607 IFLLSELGFKGDIVIETLTRVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDVVG 666
L + I+I + + V+ + RG +A ++ L + + V
Sbjct: 445 TLFSLSLADENKIIIGGSGAIPALVDLLENGTPRGKKDAATALFNLCIYHGN-KGRAVRA 504
Query: 667 SLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILGSKEGSPTVVAVLAENAIESILGSLKAK 707
++ + K D + R+ ++ +L +L + + + + A++ N + +++G L+
Sbjct: 505 GIVTALVKMLSDSTRHRMVDEALTIL---SVLANNQDAKS--AIVKANTLPALIGILQTD 564
BLAST of Carg12214 vs. ExPASy Swiss-Prot
Match:
Q9C9A6 (U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2 SV=1)
HSP 1 Score: 84.7 bits (208), Expect = 1.3e-14
Identity = 111/439 (25.28%), Postives = 195/439 (44.42%), Query Frame = 0
Query: 307 EQESSSSSRTFSIFKSTAQDQDDSSE---IFSLGSETEFEENDHEKTLFDSGQP-KPKNQ 366
++ S +S+ FS S ++D SS I L S E + ++ F+S P K +
Sbjct: 150 QRYGSLNSKKFSSGLSEPMEKDASSNRKVIEKLESIPETVHSLSDEKKFESPPPWKSSSV 209
Query: 367 KLKQTITQSLELDYAMENSRSASPESGEK--HITPPKDFVCPITCNIFYDPVTIETGQTY 426
L +++ + D +E + + + + +K ++T P+DF+CPI+ + DP + TGQTY
Sbjct: 210 SLAFFLSKDGD-DERLEKAVTENSDDSQKSDNLTIPEDFLCPISLELMKDPAIVSTGQTY 269
Query: 427 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASW-----LEENPNFVLDKAL 486
ERS IQ W+D GNL+CP T QKL+N L NYVL+ LI+ W +E+ ++ +
Sbjct: 270 ERSFIQRWIDCGNLSCPKTQQKLENFTL-TPNYVLRSLISQWCTKHNIEQPGGYMNGRTK 329
Query: 487 DEAESVAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVE 546
+ S L+ +S I RA+ +S+ +E+ TAV +
Sbjct: 330 NSDGSFRDLSGDMSAI------------RALVCKLSSQSIEDRRTAVSEIRSL---SKRS 389
Query: 547 GDVRHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCI 606
D R ++ + I LV++L + D + A+ + L + L + V I
Sbjct: 390 TDNRILIAEAGAIPVLVKLLTSDGDTETQENAVTCILNLSIYEH--NKELIMLAGAVTSI 449
Query: 607 VTLFKRGVVE----AVLLIYQLGLSSQTLQEMDVVGSLLNVVK---------KKEEDV-- 666
V + + G +E A ++ L L+ + + G+++ +V KK+
Sbjct: 450 VLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIMALVDLLQYGSVRGKKDAATAL 509
Query: 667 --------NKMRLSHKSAAVLLLRKILGSK-----EGSPTVVAVLAENAIESILGSLKAK 707
NK R L++ + S + + T+++VLA N + AK
Sbjct: 510 FNLCIYQGNKGRAVRAGIVKPLVKMLTDSSSERMADEALTILSVLASNQV--------AK 561
BLAST of Carg12214 vs. ExPASy TrEMBL
Match:
A0A6J1F0S1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441111 PE=4 SV=1)
HSP 1 Score: 2524.2 bits (6541), Expect = 0.0e+00
Identity = 1310/1343 (97.54%), Postives = 1314/1343 (97.84%), Query Frame = 0
Query: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGI+SLSDEIALKPLELAAEALE
Sbjct: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIVSLSDEIALKPLELAAEALE 60
Query: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP
Sbjct: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
Query: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTE SIGDESEVF C
Sbjct: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTEHSIGDESEVFHC 180
Query: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK
Sbjct: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
Query: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
NEKW EMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG
Sbjct: 241 NEKWNEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
Query: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK
Sbjct: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
Query: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY
Sbjct: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
Query: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES
Sbjct: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
Query: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDV+H
Sbjct: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVQH 540
Query: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK
Sbjct: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
Query: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
RGVVEAVLLIYQLGLSSQTLQEMDVVGSLL+VVKKKEEDVNKMRLSHKSAAVLLLRKILG
Sbjct: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLSVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
Query: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
SKEGSPTVVAVLAENAIESILGSLKAKQVE RIAAVGILLRCIQEDWKCRNIIADKADLA
Sbjct: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEVRIAAVGILLRCIQEDWKCRNIIADKADLA 720
Query: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH
Sbjct: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
Query: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR
Sbjct: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
Query: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL
Sbjct: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
Query: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS
Sbjct: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
Query: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
IFT+TTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC
Sbjct: 961 IFTMTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
Query: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE
Sbjct: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
Query: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQ HDVKEQIHNLVVSKSV
Sbjct: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQAHDVKEQIHNLVVSKSV 1140
Query: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
ACFIPHGVGIR VYSWAGESKLLNSSKHVKCLNLMHG
Sbjct: 1141 ACFIPHGVGIR------------------------VYSWAGESKLLNSSKHVKCLNLMHG 1200
Query: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV
Sbjct: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
Query: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK
Sbjct: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1319
Query: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1344
VVCMSLNEREDVLVIGTSDGRIQ
Sbjct: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1319
BLAST of Carg12214 vs. ExPASy TrEMBL
Match:
A0A6J1F5V1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441111 PE=4 SV=1)
HSP 1 Score: 2517.7 bits (6524), Expect = 0.0e+00
Identity = 1309/1343 (97.47%), Postives = 1313/1343 (97.77%), Query Frame = 0
Query: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGI+SLSDEIALKPLELAAEALE
Sbjct: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIVSLSDEIALKPLELAAEALE 60
Query: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP
Sbjct: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
Query: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTE SIGDESEVF C
Sbjct: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTEHSIGDESEVFHC 180
Query: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK
Sbjct: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
Query: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
NEKW EMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG
Sbjct: 241 NEKWNEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
Query: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK
Sbjct: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
Query: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY
Sbjct: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
Query: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES
Sbjct: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
Query: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDV+H
Sbjct: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVQH 540
Query: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK
Sbjct: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
Query: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
RGVVEAVLLIYQLGLSSQTLQEMDVVGSLL+VVKKKEEDVNKMRLSHKSAAVLLLRKILG
Sbjct: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLSVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
Query: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
SKEGSPTVVAVLAENAIESILGSLKAKQVE RIAAVGILLRCIQEDWKCRNIIADKADLA
Sbjct: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEVRIAAVGILLRCIQEDWKCRNIIADKADLA 720
Query: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
LILSSFTKVSNDEQFEIIMFLSELVKLN RTSNEKILRNIKDGSKCSTMHSLLTYLQTAH
Sbjct: 721 LILSSFTKVSNDEQFEIIMFLSELVKLN-RTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
Query: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR
Sbjct: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
Query: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL
Sbjct: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
Query: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS
Sbjct: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
Query: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
IFT+TTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC
Sbjct: 961 IFTMTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
Query: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE
Sbjct: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
Query: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQ HDVKEQIHNLVVSKSV
Sbjct: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQAHDVKEQIHNLVVSKSV 1140
Query: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
ACFIPHGVGIR VYSWAGESKLLNSSKHVKCLNLMHG
Sbjct: 1141 ACFIPHGVGIR------------------------VYSWAGESKLLNSSKHVKCLNLMHG 1200
Query: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV
Sbjct: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
Query: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK
Sbjct: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1318
Query: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1344
VVCMSLNEREDVLVIGTSDGRIQ
Sbjct: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1318
BLAST of Carg12214 vs. ExPASy TrEMBL
Match:
A0A6J1L0K6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499948 PE=4 SV=1)
HSP 1 Score: 2497.2 bits (6471), Expect = 0.0e+00
Identity = 1294/1343 (96.35%), Postives = 1308/1343 (97.39%), Query Frame = 0
Query: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
MVATG ITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE
Sbjct: 1 MVATGAITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
Query: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
NAIFTDNSSIKSSSLRCAEELLLS+PENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP
Sbjct: 61 NAIFTDNSSIKSSSLRCAEELLLSVPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
Query: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
SLARSEIAPILFEELFLRHFLPIIHWFH+QR +ILTFFPSNLINNTTE SIGDE EVFPC
Sbjct: 121 SLARSEIAPILFEELFLRHFLPIIHWFHQQRFQILTFFPSNLINNTTEHSIGDEPEVFPC 180
Query: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA+HFKRVLERKDNGGSIAIPMVKLLDK
Sbjct: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAEHFKRVLERKDNGGSIAIPMVKLLDK 240
Query: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
NEKWKEMEQIA+QKLRTEQLC PNGRYNPIWIEEEQTL+VD TNKSKSASPPCVRSQVFG
Sbjct: 241 NEKWKEMEQIAKQKLRTEQLCFPNGRYNPIWIEEEQTLEVDFTNKSKSASPPCVRSQVFG 300
Query: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK
Sbjct: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
Query: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY
Sbjct: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
Query: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES
Sbjct: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
Query: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEG+V+H
Sbjct: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGNVQH 540
Query: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK
Sbjct: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
Query: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
RGVVEAVLLIYQLGLSSQTLQEMD+VGSLLNVVKKKEEDVNKM LSHKSAAVLLLRKILG
Sbjct: 601 RGVVEAVLLIYQLGLSSQTLQEMDIVGSLLNVVKKKEEDVNKMHLSHKSAAVLLLRKILG 660
Query: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA
Sbjct: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
Query: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
LILSSFTKVSN EQFEIIMFLSELVKLNRRTSNEKILRNIKDGS+CSTMHSLLTYLQTAH
Sbjct: 721 LILSSFTKVSNGEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSECSTMHSLLTYLQTAH 780
Query: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
RDQCPVVAGLLLQLDILVEPLKMSIYREEAMD LISCLRDSDFPTAQISAAETIMSLQGR
Sbjct: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDVLISCLRDSDFPTAQISAAETIMSLQGR 840
Query: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
F+TSGQPLARYFLLERAGLTKNHRKPIG+ENISDALGEVELTREEDKATDEWERKMAFVL
Sbjct: 841 FSTSGQPLARYFLLERAGLTKNHRKPIGTENISDALGEVELTREEDKATDEWERKMAFVL 900
Query: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS
Sbjct: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
Query: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
IFT+TTDIE KALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC
Sbjct: 961 IFTMTTDIEEKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
Query: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSV+GTNLHLLQE
Sbjct: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVKGTNLHLLQE 1080
Query: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV
Sbjct: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
Query: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
ACFIPHGVGIR VYSWAGESKLLNSSKHVKCLNLMHG
Sbjct: 1141 ACFIPHGVGIR------------------------VYSWAGESKLLNSSKHVKCLNLMHG 1200
Query: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV
Sbjct: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
Query: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKID LQIGRNCK
Sbjct: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDALQIGRNCK 1319
Query: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1344
VVCMSLNEREDVLVIGTSDGRIQ
Sbjct: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1319
BLAST of Carg12214 vs. ExPASy TrEMBL
Match:
A0A6J1L0L5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499948 PE=4 SV=1)
HSP 1 Score: 2490.7 bits (6454), Expect = 0.0e+00
Identity = 1293/1343 (96.28%), Postives = 1307/1343 (97.32%), Query Frame = 0
Query: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
MVATG ITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE
Sbjct: 1 MVATGAITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
Query: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
NAIFTDNSSIKSSSLRCAEELLLS+PENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP
Sbjct: 61 NAIFTDNSSIKSSSLRCAEELLLSVPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
Query: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
SLARSEIAPILFEELFLRHFLPIIHWFH+QR +ILTFFPSNLINNTTE SIGDE EVFPC
Sbjct: 121 SLARSEIAPILFEELFLRHFLPIIHWFHQQRFQILTFFPSNLINNTTEHSIGDEPEVFPC 180
Query: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA+HFKRVLERKDNGGSIAIPMVKLLDK
Sbjct: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAEHFKRVLERKDNGGSIAIPMVKLLDK 240
Query: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
NEKWKEMEQIA+QKLRTEQLC PNGRYNPIWIEEEQTL+VD TNKSKSASPPCVRSQVFG
Sbjct: 241 NEKWKEMEQIAKQKLRTEQLCFPNGRYNPIWIEEEQTLEVDFTNKSKSASPPCVRSQVFG 300
Query: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK
Sbjct: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
Query: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY
Sbjct: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
Query: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES
Sbjct: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
Query: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEG+V+H
Sbjct: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGNVQH 540
Query: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK
Sbjct: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
Query: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
RGVVEAVLLIYQLGLSSQTLQEMD+VGSLLNVVKKKEEDVNKM LSHKSAAVLLLRKILG
Sbjct: 601 RGVVEAVLLIYQLGLSSQTLQEMDIVGSLLNVVKKKEEDVNKMHLSHKSAAVLLLRKILG 660
Query: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA
Sbjct: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
Query: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
LILSSFTKVSN EQFEIIMFLSELVKLN RTSNEKILRNIKDGS+CSTMHSLLTYLQTAH
Sbjct: 721 LILSSFTKVSNGEQFEIIMFLSELVKLN-RTSNEKILRNIKDGSECSTMHSLLTYLQTAH 780
Query: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
RDQCPVVAGLLLQLDILVEPLKMSIYREEAMD LISCLRDSDFPTAQISAAETIMSLQGR
Sbjct: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDVLISCLRDSDFPTAQISAAETIMSLQGR 840
Query: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
F+TSGQPLARYFLLERAGLTKNHRKPIG+ENISDALGEVELTREEDKATDEWERKMAFVL
Sbjct: 841 FSTSGQPLARYFLLERAGLTKNHRKPIGTENISDALGEVELTREEDKATDEWERKMAFVL 900
Query: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS
Sbjct: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
Query: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
IFT+TTDIE KALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC
Sbjct: 961 IFTMTTDIEEKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
Query: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSV+GTNLHLLQE
Sbjct: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVKGTNLHLLQE 1080
Query: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV
Sbjct: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
Query: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
ACFIPHGVGIR VYSWAGESKLLNSSKHVKCLNLMHG
Sbjct: 1141 ACFIPHGVGIR------------------------VYSWAGESKLLNSSKHVKCLNLMHG 1200
Query: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV
Sbjct: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
Query: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKID LQIGRNCK
Sbjct: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDALQIGRNCK 1318
Query: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1344
VVCMSLNEREDVLVIGTSDGRIQ
Sbjct: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1318
BLAST of Carg12214 vs. ExPASy TrEMBL
Match:
A0A6J1F0V1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441111 PE=4 SV=1)
HSP 1 Score: 2489.1 bits (6450), Expect = 0.0e+00
Identity = 1297/1343 (96.57%), Postives = 1301/1343 (96.87%), Query Frame = 0
Query: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60
MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGI+SLSDEIALKPLELAAEALE
Sbjct: 1 MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIVSLSDEIALKPLELAAEALE 60
Query: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP
Sbjct: 61 NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120
Query: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180
SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTE SIGDESEVF C
Sbjct: 121 SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTEHSIGDESEVFHC 180
Query: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK
Sbjct: 181 TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240
Query: 241 NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
NEKW EMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG
Sbjct: 241 NEKWNEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQTLQVDCTNKSKSASPPCVRSQVFG 300
Query: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPK 360
PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFE PKPK
Sbjct: 301 PFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFE-------------PKPK 360
Query: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY
Sbjct: 361 NQKLKQTITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIETGQTY 420
Query: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES
Sbjct: 421 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVLDKALDEAES 480
Query: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVRH 540
VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDV+H
Sbjct: 481 VAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVEGDVQH 540
Query: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK
Sbjct: 541 MLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTLFK 600
Query: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
RGVVEAVLLIYQLGLSSQTLQEMDVVGSLL+VVKKKEEDVNKMRLSHKSAAVLLLRKILG
Sbjct: 601 RGVVEAVLLIYQLGLSSQTLQEMDVVGSLLSVVKKKEEDVNKMRLSHKSAAVLLLRKILG 660
Query: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWKCRNIIADKADLA 720
SKEGSPTVVAVLAENAIESILGSLKAKQVE RIAAVGILLRCIQEDWKCRNIIADKADLA
Sbjct: 661 SKEGSPTVVAVLAENAIESILGSLKAKQVEVRIAAVGILLRCIQEDWKCRNIIADKADLA 720
Query: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH
Sbjct: 721 LILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCSTMHSLLTYLQTAH 780
Query: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR
Sbjct: 781 RDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQISAAETIMSLQGR 840
Query: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL
Sbjct: 841 FNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTREEDKATDEWERKMAFVL 900
Query: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS
Sbjct: 901 VNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGILEAARIFLLDHFLS 960
Query: 961 IFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
IFT+TTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC
Sbjct: 961 IFTMTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRRSTPLAFDMLKVLC 1020
Query: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE
Sbjct: 1021 EGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTMKVWSVRGTNLHLLQE 1080
Query: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVHDVKEQIHNLVVSKSV 1140
IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQ HDVKEQIHNLVVSKSV
Sbjct: 1081 IQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQAHDVKEQIHNLVVSKSV 1140
Query: 1141 ACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKLLNSSKHVKCLNLMHG 1200
ACFIPHGVGIR VYSWAGESKLLNSSKHVKCLNLMHG
Sbjct: 1141 ACFIPHGVGIR------------------------VYSWAGESKLLNSSKHVKCLNLMHG 1200
Query: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV
Sbjct: 1201 KLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAV 1260
Query: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1320
KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK
Sbjct: 1261 KIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCK 1306
Query: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1344
VVCMSLNEREDVLVIGTSDGRIQ
Sbjct: 1321 VVCMSLNEREDVLVIGTSDGRIQ 1306
BLAST of Carg12214 vs. TAIR 10
Match:
AT3G06880.2 (Transducin/WD40 repeat-like superfamily protein )
HSP 1 Score: 211.1 bits (536), Expect = 8.3e-54
Identity = 197/822 (23.97%), Postives = 377/822 (45.86%), Query Frame = 0
Query: 539 RHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTL 598
++ +LK +++ L + +S + V++A++ L+++ +E + R +++ +
Sbjct: 478 KYSMLKDVILDQLFTAISSSEEKTVIKASMTALTKIISVNRTALEEVKRKGLNLSHLANA 537
Query: 599 FKRGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEED-------VNKMRLSHKSAA 658
K+ V EA +LIY + S ++ ++++ +L++VV + L+ +A+
Sbjct: 538 LKQNVQEAAILIYLIKPSPTEIKSLELLPALVDVVASTSSSSSCYTFIPSPSYLTPPAAS 597
Query: 659 VLLLRKILGSKEGSPT---VVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWK 718
++++ ++ + + + + A+ + + + +L K+ E I+ IL++C+Q D
Sbjct: 598 LMIIEVLITAFDHATNTMHLAAISSPSVLCGLLDVAKSGNSGEFISLTSILVKCMQFDGL 657
Query: 719 CRNIIADKADLALILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCST 778
R I +A +E + FL E++K+ R+S KIL+ IK
Sbjct: 658 HRKYIYQHTRVAPFAHLLQSKDQEEICIALQFLHEVLKI-PRSSAIKILQQIKKEGSFDI 717
Query: 779 MHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQI 838
+LL ++ D A +LLQL+ L P + YR EA AL+ + S+ Q+
Sbjct: 718 KVTLLHCIKHLQGDHKLFAADILLQLNALDSPPENKKYRNEATRALLDAVTYSEGSNMQL 777
Query: 839 SAAETIMSLQGRFNTSGQPLARYFLLERAGLTK-NHRKPIGSENISDALGEVELTREEDK 898
+ + ++ G ++ +G+P +L++R GLT +H I + N SD +D
Sbjct: 778 LSTFILANIGGTYSWTGEPYTAAWLMKRGGLTSMSHMNMIRNINWSDEC-------LQDT 837
Query: 899 ATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGIL 958
D W K+A +++ L +GLKSK ++ AC ++ WLS + P++
Sbjct: 838 GIDGWCCKIARRIIDTGKATFC-GLQEGLKSKNKSVSKACLIAIAWLSIEISKGPNSLKY 897
Query: 959 EAARIFLLDHFLSIFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRR 1018
A + LLD ++E + LA + I +F +G+ L + + + L+
Sbjct: 898 SACEV-LLDEVAQFLHPGLELEERLLACICIYNF-SSGKGIHKLVNFSEGVRESLRRLSH 957
Query: 1019 STPLAFDMLKVLCEGQDSSTELWS--HHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTM 1078
T +A ++ K S + S H + ++ S +G V ++ + K + SG SDG++
Sbjct: 958 VTWMADELHKATYYLFSKSDQRISCVHTQTVEMHQSGSGAVTALIYHKGLLFSGFSDGSI 1017
Query: 1079 KVWSVRGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVH 1138
+VW+V LL +I+EH VT +++E+ E + SGS DKTI+VW + ++C +V
Sbjct: 1018 RVWNVNKKIATLLWDIKEHKSTVTCFSLSETGECVLSGSADKTIRVWQIVKGKLECAEVI 1077
Query: 1139 DVKEQIHNLVVSKSVACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKL 1198
K+ I L ++ I G +++ + S+
Sbjct: 1078 KTKDSIRKLEAFGNMIFVITKGHKMKLLDS------------------------SRISQS 1137
Query: 1199 LNSSKHVKCLNLMHGKLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYD 1258
+ K VK + GK+Y GC D+SIQE+ +A I + +R + PI+++ VY
Sbjct: 1138 IFKGKGVKSMVSAQGKIYIGCIDTSIQELIVANKREKEIKAPTRSWRLQNKPINSVVVYK 1197
Query: 1259 EQLFSASTALDGAAVKIRCRSNYGVIGSLST--AMDVRTMAVGSNLTYL--GGKGGVVEI 1318
+ L+S+ST ++ + +K R NY S++ ++ M V + YL ++I
Sbjct: 1198 DMLYSSSTYVEMSNIK-DLRRNYEPQMSITAEKGSNIVAMGVVEDFIYLNRSSSANTLQI 1257
Query: 1319 WSREKHNKIDTLQIGRNCKVVCMSLNEREDVLVIGTSDGRIQ 1344
W R K+ L G SL D++ GT G I+
Sbjct: 1258 WLRRTQQKVGRLSAGSK----ITSLLTANDIVFCGTEAGVIK 1259
BLAST of Carg12214 vs. TAIR 10
Match:
AT3G06880.1 (Transducin/WD40 repeat-like superfamily protein )
HSP 1 Score: 210.3 bits (534), Expect = 1.4e-53
Identity = 196/819 (23.93%), Postives = 375/819 (45.79%), Query Frame = 0
Query: 539 RHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCIVTL 598
++ +LK +++ L + +S + V++A++ L+++ +E + R +++ +
Sbjct: 478 KYSMLKDVILDQLFTAISSSEEKTVIKASMTALTKIISVNRTALEEVKRKGLNLSHLANA 537
Query: 599 FKRGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEED-------VNKMRLSHKSAA 658
K+ V EA +LIY + S ++ ++++ +L++VV + L+ +A+
Sbjct: 538 LKQNVQEAAILIYLIKPSPTEIKSLELLPALVDVVASTSSSSSCYTFIPSPSYLTPPAAS 597
Query: 659 VLLLRKILGSKEGSPT---VVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQEDWK 718
++++ ++ + + + + A+ + + + +L K+ E I+ IL++C+Q D
Sbjct: 598 LMIIEVLITAFDHATNTMHLAAISSPSVLCGLLDVAKSGNSGEFISLTSILVKCMQFDGL 657
Query: 719 CRNIIADKADLALILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSKCST 778
R I +A +E + FL E++K+ R+S KIL+ IK
Sbjct: 658 HRKYIYQHTRVAPFAHLLQSKDQEEICIALQFLHEVLKI-PRSSAIKILQQIKKEGSFDI 717
Query: 779 MHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPTAQI 838
+LL ++ D A +LLQL+ L P + YR EA AL+ + S+ Q+
Sbjct: 718 KVTLLHCIKHLQGDHKLFAADILLQLNALDSPPENKKYRNEATRALLDAVTYSEGSNMQL 777
Query: 839 SAAETIMSLQGRFNTSGQPLARYFLLERAGLTK-NHRKPIGSENISDALGEVELTREEDK 898
+ + ++ G ++ +G+P +L++R GLT +H I + N SD +D
Sbjct: 778 LSTFILANIGGTYSWTGEPYTAAWLMKRGGLTSMSHMNMIRNINWSDEC-------LQDT 837
Query: 899 ATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTLPDTGIL 958
D W K+A +++ L +GLKSK ++ AC ++ WLS + P++
Sbjct: 838 GIDGWCCKIARRIIDTGKATFC-GLQEGLKSKNKSVSKACLIAIAWLSIEISKGPNSLKY 897
Query: 959 EAARIFLLDHFLSIFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRGLKEFRR 1018
A + LLD ++E + LA + I +F +G+ L + + + L+
Sbjct: 898 SACEV-LLDEVAQFLHPGLELEERLLACICIYNF-SSGKGIHKLVNFSEGVRESLRRLSH 957
Query: 1019 STPLAFDMLKVLCEGQDSSTELWS--HHELFKVDCSRNGEVLSIAHCKDRIISGHSDGTM 1078
T +A ++ K S + S H + ++ S +G V ++ + K + SG SDG++
Sbjct: 958 VTWMADELHKATYYLFSKSDQRISCVHTQTVEMHQSGSGAVTALIYHKGLLFSGFSDGSI 1017
Query: 1079 KVWSVRGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQCIQVH 1138
+VW+V LL +I+EH VT +++E+ E + SGS DKTI+VW + ++C +V
Sbjct: 1018 RVWNVNKKIATLLWDIKEHKSTVTCFSLSETGECVLSGSADKTIRVWQIVKGKLECAEVI 1077
Query: 1139 DVKEQIHNLVVSKSVACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAGESKL 1198
K+ I L ++ I G +++ + S+
Sbjct: 1078 KTKDSIRKLEAFGNMIFVITKGHKMKLLDS------------------------SRISQS 1137
Query: 1199 LNSSKHVKCLNLMHGKLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYD 1258
+ K VK + GK+Y GC D+SIQE+ +A I + +R + PI+++ VY
Sbjct: 1138 IFKGKGVKSMVSAQGKIYIGCIDTSIQELIVANKREKEIKAPTRSWRLQNKPINSVVVYK 1197
Query: 1259 EQLFSASTALDGAAVKIRCRSNYGVIGSLST--AMDVRTMAVGSNLTYL--GGKGGVVEI 1318
+ L+S+ST ++ + +K R NY S++ ++ M V + YL ++I
Sbjct: 1198 DMLYSSSTYVEMSNIK-DLRRNYEPQMSITAEKGSNIVAMGVVEDFIYLNRSSSANTLQI 1256
Query: 1319 WSREKHNKIDTLQIGRNCKVVCMSLNEREDVLVIGTSDG 1341
W R K+ L G SL D++ GT G
Sbjct: 1258 WLRRTQQKVGRLSAGSK----ITSLLTANDIVFCGTEAG 1256
BLAST of Carg12214 vs. TAIR 10
Match:
AT1G23030.1 (ARM repeat superfamily protein )
HSP 1 Score: 101.3 bits (251), Expect = 9.3e-21
Identity = 107/439 (24.37%), Postives = 197/439 (44.87%), Query Frame = 0
Query: 307 EQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDSGQPKPKNQKLKQ 366
++ S +S FS S ++D S + + +E + E+ E F + K + L++
Sbjct: 145 QRYGSLNSNKFSSALSEPMERDGFSNVIKIKAEEKL-ESVSETLHFGEEEEKQSSPPLRR 204
Query: 367 TITQSL-----------ELDYAMENSRSASPESGEKHITPPKDFVCPITCNIFYDPVTIE 426
+ + SL LD + + S +S + +T P DF+CP++ + DPV +
Sbjct: 205 SSSISLAYYLSKDADTDRLDKMVNKNTDESKKSDK--LTIPVDFLCPVSLELMKDPVIVA 264
Query: 427 TGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEEN----PNFVL 486
TGQTYER+ IQ W+D GNLTCP T QKL+N L NYVL+ LI+ W E+ P +
Sbjct: 265 TGQTYERAYIQRWIDCGNLTCPKTQQKLENFTL-TPNYVLRSLISRWCAEHNIEQPAGYI 324
Query: 487 DKALDEAESVAVLTSPVSFISQASID---------RSMEMRR--------------AITN 546
+ + ++V+ + V +S S + RS+ R + N
Sbjct: 325 NGRTKNSGDMSVIRALVQRLSSRSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVN 384
Query: 547 LCTSEVLEEAETAVLCVERFWLEENVEGDVRHMLLKPSVINGLVEIL-VNSVDLQVLRAA 606
L TSE + E A+ CV + EN + +++ + +V++L +++ + AA
Sbjct: 385 LLTSEDVATQENAITCVLNLSIYEN----NKELIMFAGAVTSIVQVLRAGTMEARENAAA 444
Query: 607 IFLLSELGFKGDIVIETLTRVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDVVG 666
L + I+I + + V+ + RG +A ++ L + + V
Sbjct: 445 TLFSLSLADENKIIIGGSGAIPALVDLLENGTPRGKKDAATALFNLCIYHGN-KGRAVRA 504
Query: 667 SLLNVVKKKEEDVNKMRLSHKSAAVLLLRKILGSKEGSPTVVAVLAENAIESILGSLKAK 707
++ + K D + R+ ++ +L +L + + + + A++ N + +++G L+
Sbjct: 505 GIVTALVKMLSDSTRHRMVDEALTIL---SVLANNQDAKS--AIVKANTLPALIGILQTD 564
BLAST of Carg12214 vs. TAIR 10
Match:
AT1G71020.1 (ARM repeat superfamily protein )
HSP 1 Score: 84.7 bits (208), Expect = 9.0e-16
Identity = 111/439 (25.28%), Postives = 195/439 (44.42%), Query Frame = 0
Query: 307 EQESSSSSRTFSIFKSTAQDQDDSSE---IFSLGSETEFEENDHEKTLFDSGQP-KPKNQ 366
++ S +S+ FS S ++D SS I L S E + ++ F+S P K +
Sbjct: 150 QRYGSLNSKKFSSGLSEPMEKDASSNRKVIEKLESIPETVHSLSDEKKFESPPPWKSSSV 209
Query: 367 KLKQTITQSLELDYAMENSRSASPESGEK--HITPPKDFVCPITCNIFYDPVTIETGQTY 426
L +++ + D +E + + + + +K ++T P+DF+CPI+ + DP + TGQTY
Sbjct: 210 SLAFFLSKDGD-DERLEKAVTENSDDSQKSDNLTIPEDFLCPISLELMKDPAIVSTGQTY 269
Query: 427 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASW-----LEENPNFVLDKAL 486
ERS IQ W+D GNL+CP T QKL+N L NYVL+ LI+ W +E+ ++ +
Sbjct: 270 ERSFIQRWIDCGNLSCPKTQQKLENFTL-TPNYVLRSLISQWCTKHNIEQPGGYMNGRTK 329
Query: 487 DEAESVAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVE 546
+ S L+ +S I RA+ +S+ +E+ TAV +
Sbjct: 330 NSDGSFRDLSGDMSAI------------RALVCKLSSQSIEDRRTAVSEIRSL---SKRS 389
Query: 547 GDVRHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCI 606
D R ++ + I LV++L + D + A+ + L + L + V I
Sbjct: 390 TDNRILIAEAGAIPVLVKLLTSDGDTETQENAVTCILNLSIYEH--NKELIMLAGAVTSI 449
Query: 607 VTLFKRGVVE----AVLLIYQLGLSSQTLQEMDVVGSLLNVVK---------KKEEDV-- 666
V + + G +E A ++ L L+ + + G+++ +V KK+
Sbjct: 450 VLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIMALVDLLQYGSVRGKKDAATAL 509
Query: 667 --------NKMRLSHKSAAVLLLRKILGSK-----EGSPTVVAVLAENAIESILGSLKAK 707
NK R L++ + S + + T+++VLA N + AK
Sbjct: 510 FNLCIYQGNKGRAVRAGIVKPLVKMLTDSSSERMADEALTILSVLASNQV--------AK 561
BLAST of Carg12214 vs. TAIR 10
Match:
AT1G71020.2 (ARM repeat superfamily protein )
HSP 1 Score: 84.7 bits (208), Expect = 9.0e-16
Identity = 111/439 (25.28%), Postives = 195/439 (44.42%), Query Frame = 0
Query: 307 EQESSSSSRTFSIFKSTAQDQDDSSE---IFSLGSETEFEENDHEKTLFDSGQP-KPKNQ 366
++ S +S+ FS S ++D SS I L S E + ++ F+S P K +
Sbjct: 2 QRYGSLNSKKFSSGLSEPMEKDASSNRKVIEKLESIPETVHSLSDEKKFESPPPWKSSSV 61
Query: 367 KLKQTITQSLELDYAMENSRSASPESGEK--HITPPKDFVCPITCNIFYDPVTIETGQTY 426
L +++ + D +E + + + + +K ++T P+DF+CPI+ + DP + TGQTY
Sbjct: 62 SLAFFLSKDGD-DERLEKAVTENSDDSQKSDNLTIPEDFLCPISLELMKDPAIVSTGQTY 121
Query: 427 ERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASW-----LEENPNFVLDKAL 486
ERS IQ W+D GNL+CP T QKL+N L NYVL+ LI+ W +E+ ++ +
Sbjct: 122 ERSFIQRWIDCGNLSCPKTQQKLENFTL-TPNYVLRSLISQWCTKHNIEQPGGYMNGRTK 181
Query: 487 DEAESVAVLTSPVSFISQASIDRSMEMRRAITNLCTSEVLEEAETAVLCVERFWLEENVE 546
+ S L+ +S I RA+ +S+ +E+ TAV +
Sbjct: 182 NSDGSFRDLSGDMSAI------------RALVCKLSSQSIEDRRTAVSEIRSL---SKRS 241
Query: 547 GDVRHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLTRVESDVNCI 606
D R ++ + I LV++L + D + A+ + L + L + V I
Sbjct: 242 TDNRILIAEAGAIPVLVKLLTSDGDTETQENAVTCILNLSIYEH--NKELIMLAGAVTSI 301
Query: 607 VTLFKRGVVE----AVLLIYQLGLSSQTLQEMDVVGSLLNVVK---------KKEEDV-- 666
V + + G +E A ++ L L+ + + G+++ +V KK+
Sbjct: 302 VLVLRAGSMEARENAAATLFSLSLADENKIIIGASGAIMALVDLLQYGSVRGKKDAATAL 361
Query: 667 --------NKMRLSHKSAAVLLLRKILGSK-----EGSPTVVAVLAENAIESILGSLKAK 707
NK R L++ + S + + T+++VLA N + AK
Sbjct: 362 FNLCIYQGNKGRAVRAGIVKPLVKMLTDSSSERMADEALTILSVLASNQV--------AK 413
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
KAG7021867.1 | 0.0e+00 | 100.00 | putative E3 ubiquitin-protein ligase LIN-1, partial [Cucurbita argyrosperma subs... | [more] |
XP_022933804.1 | 0.0e+00 | 97.54 | putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Cucurbita moschata] | [more] |
XP_022933805.1 | 0.0e+00 | 97.47 | putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Cucurbita moschata] | [more] |
XP_023007456.1 | 0.0e+00 | 96.35 | putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Cucurbita maxima] | [more] |
XP_023007466.1 | 0.0e+00 | 96.28 | putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
D1FP53 | 9.2e-199 | 33.47 | Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN P... | [more] |
C6L7U1 | 1.5e-193 | 39.64 | Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBER... | [more] |
D1FP57 | 5.2e-186 | 37.94 | Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE... | [more] |
Q8GUG9 | 1.3e-19 | 24.37 | U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2... | [more] |
Q9C9A6 | 1.3e-14 | 25.28 | U-box domain-containing protein 10 OS=Arabidopsis thaliana OX=3702 GN=PUB10 PE=2... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1F0S1 | 0.0e+00 | 97.54 | RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441111... | [more] |
A0A6J1F5V1 | 0.0e+00 | 97.47 | RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441111... | [more] |
A0A6J1L0K6 | 0.0e+00 | 96.35 | RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499948 P... | [more] |
A0A6J1L0L5 | 0.0e+00 | 96.28 | RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499948 P... | [more] |
A0A6J1F0V1 | 0.0e+00 | 96.57 | RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441111... | [more] |