Carg12173 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg12173
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAcetylglutamate kinase
LocationCarg_Chr11: 2556606 .. 2557652 (+)
RNA-Seq ExpressionCarg12173
SyntenyCarg12173
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGACGATCGAAAGCTTGCTCAATCCATCCGCCTTCAACCTATTTTCCTCCAAATCCTTGTTCAATCCTTCCATTTCCAGTTCCTCCATTTTCTCGCCCGCTCCGCGATTGCGCAGAATAAGTTCTTCTTCGTCGCTGTCGATTAGGTGCTCGGCAACTCAGCAAATGGAGGCTGTGGTTGATTCTGTAAGTCCAGAGCAGTTCCGAGTAGATGTTCTCTCGGAATCACTGCCATTCATACAGAAATTTCGACGTAAAACGATTGTTGTGAAGTACGGTGGGGCCGCTATGAAATCGCGGTCGCTCCAGGCTTCGGTTGTTAATGATCTTGTACTTCTGTCCTGCGTCGGCCTTCGTCCGATCTTGGTCCATGGCGGCGGGCCGGAAATCAACAACTGGCTCAAACGGCTCAACATTGAGGTCGTTTTCTGCGACGGTCTTCGCGTGACCGACGCCGAAACGATGAAAATAGTGTCGATGGTGTTGGTCGGCGACGTGAATAAGAACCTGGTTTCGTTGATCAATAAGGCCGGAGCATCGGCTGCAGGGCTCTGCGGTGTGGATGGGCGGCTACTGACGGCTCGTCCAGCCCCTAACGCGGCACAGTTAGGATTTGTAGGCGAGGTCGCACGTGTGGATCCTACGGTTCTACAGGCACTTCTGGACAAAGGCCACATTCCGGTGGTTTCTTCGGTGGCTGCCGATGAGTCGGGGCAGATGTACAATATCAACGCGGACACAGTTGCCGGAGAATTAGCGGCGGCGCTGGGAGCGGAGAAGCTGATCTTACTGACGGATGTGGCGGGGATTCTGGAAGACCGTGACGATCCGAAAAGCTTAGTGAAGCAGATAGACATAAAAGGAGTGAAGAAGATGATGGAAGAGGGAAGAATCGGCGGAGGGATGATTCCAAAGGTGAATTGTTGTGTCCGATCGTTGGCTCAAGGGGTGAGGACGGCGAGTATCATTGACGGGAGGCTGGAGCATTCGTTGCTGCTAGAGATCATGACTGACAATGGAGCCGGAACCATGATTATAGGCTAA

mRNA sequence

ATGCCGACGATCGAAAGCTTGCTCAATCCATCCGCCTTCAACCTATTTTCCTCCAAATCCTTGTTCAATCCTTCCATTTCCAGTTCCTCCATTTTCTCGCCCGCTCCGCGATTGCGCAGAATAAGTTCTTCTTCGTCGCTGTCGATTAGGTGCTCGGCAACTCAGCAAATGGAGGCTGTGGTTGATTCTGTAAGTCCAGAGCAGTTCCGAGTAGATGTTCTCTCGGAATCACTGCCATTCATACAGAAATTTCGACGTAAAACGATTGTTGTGAAGTACGGTGGGGCCGCTATGAAATCGCGGTCGCTCCAGGCTTCGGTTGTTAATGATCTTGTACTTCTGTCCTGCGTCGGCCTTCGTCCGATCTTGGTCCATGGCGGCGGGCCGGAAATCAACAACTGGCTCAAACGGCTCAACATTGAGGTCGTTTTCTGCGACGGTCTTCGCGTGACCGACGCCGAAACGATGAAAATAGTGTCGATGGTGTTGGTCGGCGACGTGAATAAGAACCTGGTTTCGTTGATCAATAAGGCCGGAGCATCGGCTGCAGGGCTCTGCGGTGTGGATGGGCGGCTACTGACGGCTCGTCCAGCCCCTAACGCGGCACAGTTAGGATTTGTAGGCGAGGTCGCACGTGTGGATCCTACGGTTCTACAGGCACTTCTGGACAAAGGCCACATTCCGGTGGTTTCTTCGGTGGCTGCCGATGAGTCGGGGCAGATGTACAATATCAACGCGGACACAGTTGCCGGAGAATTAGCGGCGGCGCTGGGAGCGGAGAAGCTGATCTTACTGACGGATGTGGCGGGGATTCTGGAAGACCGTGACGATCCGAAAAGCTTAGTGAAGCAGATAGACATAAAAGGAGTGAAGAAGATGATGGAAGAGGGAAGAATCGGCGGAGGGATGATTCCAAAGGTGAATTGTTGTGTCCGATCGTTGGCTCAAGGGGTGAGGACGGCGAGTATCATTGACGGGAGGCTGGAGCATTCGTTGCTGCTAGAGATCATGACTGACAATGGAGCCGGAACCATGATTATAGGCTAA

Coding sequence (CDS)

ATGCCGACGATCGAAAGCTTGCTCAATCCATCCGCCTTCAACCTATTTTCCTCCAAATCCTTGTTCAATCCTTCCATTTCCAGTTCCTCCATTTTCTCGCCCGCTCCGCGATTGCGCAGAATAAGTTCTTCTTCGTCGCTGTCGATTAGGTGCTCGGCAACTCAGCAAATGGAGGCTGTGGTTGATTCTGTAAGTCCAGAGCAGTTCCGAGTAGATGTTCTCTCGGAATCACTGCCATTCATACAGAAATTTCGACGTAAAACGATTGTTGTGAAGTACGGTGGGGCCGCTATGAAATCGCGGTCGCTCCAGGCTTCGGTTGTTAATGATCTTGTACTTCTGTCCTGCGTCGGCCTTCGTCCGATCTTGGTCCATGGCGGCGGGCCGGAAATCAACAACTGGCTCAAACGGCTCAACATTGAGGTCGTTTTCTGCGACGGTCTTCGCGTGACCGACGCCGAAACGATGAAAATAGTGTCGATGGTGTTGGTCGGCGACGTGAATAAGAACCTGGTTTCGTTGATCAATAAGGCCGGAGCATCGGCTGCAGGGCTCTGCGGTGTGGATGGGCGGCTACTGACGGCTCGTCCAGCCCCTAACGCGGCACAGTTAGGATTTGTAGGCGAGGTCGCACGTGTGGATCCTACGGTTCTACAGGCACTTCTGGACAAAGGCCACATTCCGGTGGTTTCTTCGGTGGCTGCCGATGAGTCGGGGCAGATGTACAATATCAACGCGGACACAGTTGCCGGAGAATTAGCGGCGGCGCTGGGAGCGGAGAAGCTGATCTTACTGACGGATGTGGCGGGGATTCTGGAAGACCGTGACGATCCGAAAAGCTTAGTGAAGCAGATAGACATAAAAGGAGTGAAGAAGATGATGGAAGAGGGAAGAATCGGCGGAGGGATGATTCCAAAGGTGAATTGTTGTGTCCGATCGTTGGCTCAAGGGGTGAGGACGGCGAGTATCATTGACGGGAGGCTGGAGCATTCGTTGCTGCTAGAGATCATGACTGACAATGGAGCCGGAACCATGATTATAGGCTAA

Protein sequence

MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAVVDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Homology
BLAST of Carg12173 vs. NCBI nr
Match: KAG7021826.1 (Acetylglutamate kinase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 655.6 bits (1690), Expect = 2.4e-184
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV
Sbjct: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
           VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR
Sbjct: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
           PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA
Sbjct: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ
Sbjct: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
           MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG
Sbjct: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Sbjct: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of Carg12173 vs. NCBI nr
Match: XP_022932833.1 (acetylglutamate kinase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 646.0 bits (1665), Expect = 1.9e-181
Identity = 344/348 (98.85%), Postives = 345/348 (99.14%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRR+SSSSSLSIRCSATQQMEAV
Sbjct: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRLSSSSSLSIRCSATQQMEAV 60

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
           VDSVSPEQFRVDVLSESLPFIQKFR KTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR
Sbjct: 61  VDSVSPEQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
           PILVHGGGPEINNWLKRLNIEVVF DGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA
Sbjct: 121 PILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIP VSSVAADESGQ
Sbjct: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPAVSSVAADESGQ 240

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
           MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG
Sbjct: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Sbjct: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of Carg12173 vs. NCBI nr
Match: XP_023531202.1 (acetylglutamate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023531203.1 acetylglutamate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023531204.1 acetylglutamate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_023531205.1 acetylglutamate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645.2 bits (1663), Expect = 3.2e-181
Identity = 345/348 (99.14%), Postives = 345/348 (99.14%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           M TIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV
Sbjct: 1   MSTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
           VDSVSPEQFRVDVLSESLPFIQKFR KTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR
Sbjct: 61  VDSVSPEQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
           PILVHGGGPEINNWLKRLNIEVVF DGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA
Sbjct: 121 PILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ
Sbjct: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
           MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG
Sbjct: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Sbjct: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of Carg12173 vs. NCBI nr
Match: KAG6587937.1 (Acetylglutamate kinase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 641.0 bits (1652), Expect = 6.1e-180
Identity = 342/348 (98.28%), Postives = 343/348 (98.56%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSAT+QMEAV
Sbjct: 37  MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATRQMEAV 96

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
           VDSVSPEQFRVDVLSESLPFIQKFR KTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR
Sbjct: 97  VDSVSPEQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 156

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
            ILVHGG PEINNWLKRLNIEV FCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA
Sbjct: 157 SILVHGGRPEINNWLKRLNIEVFFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 216

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           SAAGLCGVDGRLLTARPAPNAAQ GFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ
Sbjct: 217 SAAGLCGVDGRLLTARPAPNAAQSGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 276

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
           MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG
Sbjct: 277 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 336

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Sbjct: 337 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 384

BLAST of Carg12173 vs. NCBI nr
Match: XP_023001499.1 (acetylglutamate kinase, chloroplastic-like [Cucurbita maxima] >XP_023001506.1 acetylglutamate kinase, chloroplastic-like [Cucurbita maxima] >XP_023001514.1 acetylglutamate kinase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 639.4 bits (1648), Expect = 1.8e-179
Identity = 342/348 (98.28%), Postives = 342/348 (98.28%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           MP IESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISS SSLSIRCSAT QMEAV
Sbjct: 1   MPKIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSPSSLSIRCSATHQMEAV 60

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
           VDSVSPEQFRVDVLSESLPFIQKFR KTIVVKYGGAAMKS SLQASVVNDLVLLSCVGLR
Sbjct: 61  VDSVSPEQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSLSLQASVVNDLVLLSCVGLR 120

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
           PILVHGGGPEINNWLKRLNIEVVF DGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA
Sbjct: 121 PILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ
Sbjct: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
           MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG
Sbjct: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Sbjct: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of Carg12173 vs. ExPASy Swiss-Prot
Match: Q9SCL7 (Acetylglutamate kinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NAGK PE=1 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.3e-120
Identity = 233/348 (66.95%), Postives = 278/348 (79.89%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           M T+ S  +P +F+ F+  + F   I + S     P   +       SI+ + +      
Sbjct: 1   MATVTSNASPKSFS-FTVSNPFKTLIPNKSPSLCYPTRNKNHHRLGFSIKATVSTPPSIA 60

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
             +     +RV++LSESLPFIQKFR KTIVVKYGGAAM S  L++SVV+DLVLL+CVGLR
Sbjct: 61  TGNAPSPDYRVEILSESLPFIQKFRGKTIVVKYGGAAMTSPELKSSVVSDLVLLACVGLR 120

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
           PILVHGGGP+IN +LK+LNI   F DGLRVTDA TM+IVSMVLVG VNKNLVSLIN AGA
Sbjct: 121 PILVHGGGPDINRYLKQLNIPAEFRDGLRVTDATTMEIVSMVLVGKVNKNLVSLINAAGA 180

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           +A GL G DGRLLTARP PN+AQLGFVGEVARVDP+VL+ L+D G+IPV++SVAAD+SGQ
Sbjct: 181 TAVGLSGHDGRLLTARPVPNSAQLGFVGEVARVDPSVLRPLVDYGYIPVIASVAADDSGQ 240

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
            YNINADTVAGELAAALGAEKLILLTDVAGILE+++DP SL+K+IDIKGVKKM+E+G++ 
Sbjct: 241 AYNINADTVAGELAAALGAEKLILLTDVAGILENKEDPSSLIKEIDIKGVKKMIEDGKVA 300

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKV CC+RSLAQGV+TASIIDGR +HSLL EIM+D GAGTMI G
Sbjct: 301 GGMIPKVKCCIRSLAQGVKTASIIDGRRQHSLLHEIMSDEGAGTMITG 347

BLAST of Carg12173 vs. ExPASy Swiss-Prot
Match: Q8YXG8 (Acetylglutamate kinase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=argB PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 9.4e-99
Identity = 185/282 (65.60%), Postives = 222/282 (78.72%), Query Frame = 0

Query: 67  EQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHG 126
           E  RV VLSE+LP+IQ+F  +T+VVKYGGAAMK   L+  V+ D+V LSCVGLRPILVHG
Sbjct: 13  EATRVQVLSEALPYIQQFAGRTVVVKYGGAAMKDSHLKDQVIRDIVFLSCVGLRPILVHG 72

Query: 127 GGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGASAAGLC 186
           GGPEIN+WL +L IE  F +GLRVTDA TM +V MVLVG VNK +VSLIN+AG  A GLC
Sbjct: 73  GGPEINSWLDKLGIEAQFKNGLRVTDAPTMDVVEMVLVGRVNKEIVSLINQAGGLAVGLC 132

Query: 187 GVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINA 246
           G DG L+TARP      +GFVGEV+ V+  +L+ L   G+IPVVSSVAAD+SGQ YNINA
Sbjct: 133 GKDGNLITARPQGQEG-IGFVGEVSNVNIKILETLASNGYIPVVSSVAADDSGQAYNINA 192

Query: 247 DTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPK 306
           DTVAGE+AAALGAEKLILLTD  GIL+D  DP +L+ ++DI+  ++++  G + GGMIPK
Sbjct: 193 DTVAGEIAAALGAEKLILLTDTRGILKDYQDPGTLIPKVDIREARELINSGVVSGGMIPK 252

Query: 307 VNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           V CCVRSLAQGVR A IIDGR+ H+LLLEI TD G GTMI+G
Sbjct: 253 VTCCVRSLAQGVRAAHIIDGRIPHALLLEIFTDVGIGTMILG 293

BLAST of Carg12173 vs. ExPASy Swiss-Prot
Match: Q3MFQ8 (Acetylglutamate kinase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=argB PE=3 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 2.1e-98
Identity = 185/282 (65.60%), Postives = 222/282 (78.72%), Query Frame = 0

Query: 67  EQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHG 126
           E  RV VLSE+LP+IQ+F  +T+VVKYGGAAMK   L+  V+ D+V LSCVGLRPILVHG
Sbjct: 13  EATRVQVLSEALPYIQQFAGRTVVVKYGGAAMKDSHLKDQVIRDIVFLSCVGLRPILVHG 72

Query: 127 GGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGASAAGLC 186
           GGPEIN+WL +L IE  F +GLRVTDA TM +V MVLVG VNK +VSLIN+AG  A GLC
Sbjct: 73  GGPEINSWLDKLGIEAQFKNGLRVTDAPTMDVVEMVLVGRVNKEIVSLINQAGGLAVGLC 132

Query: 187 GVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINA 246
           G DG L+TARP      +GFVGEV+ V+  +L+ L   G+IPVVSSVAAD+SGQ YNINA
Sbjct: 133 GKDGNLITARPQGQEG-IGFVGEVSNVNIKILETLASNGYIPVVSSVAADDSGQAYNINA 192

Query: 247 DTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPK 306
           DTVAGE+AAALGAEKLILLTD  GIL+D  DP +L+ ++DI+  ++++  G + GGMIPK
Sbjct: 193 DTVAGEIAAALGAEKLILLTDTRGILKDYKDPGTLIPKVDIREARELINGGVVSGGMIPK 252

Query: 307 VNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           V CCVRSLAQGVR A IIDGR+ H+LLLEI TD G GTMI+G
Sbjct: 253 VTCCVRSLAQGVRAAHIIDGRIPHALLLEIFTDVGIGTMILG 293

BLAST of Carg12173 vs. ExPASy Swiss-Prot
Match: B0CAM3 (Acetylglutamate kinase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=argB PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.8e-97
Identity = 181/277 (65.34%), Postives = 221/277 (79.78%), Query Frame = 0

Query: 70  RVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHGGGP 129
           RV VLSE+LP+IQ F  +T+VVKYGGAAMK  +L+A+V+ D+V ++CVGLRP+LVHGGGP
Sbjct: 7   RVQVLSEALPYIQSFAGRTVVVKYGGAAMKDSTLKATVIRDVVFMACVGLRPVLVHGGGP 66

Query: 130 EINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGASAAGLCGVD 189
           EIN WL++L IE  F  GLRVTDA TM +V MVLVG VNK LVSLIN+AG SA G+CG D
Sbjct: 67  EINLWLEKLGIEPQFMKGLRVTDAPTMDVVEMVLVGRVNKELVSLINQAGGSAVGVCGKD 126

Query: 190 GRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINADTV 249
           G+LLTARP   +  +GFVGEVA V+P ++  LLD GHIP+VSSVA DE GQ+YNINADTV
Sbjct: 127 GKLLTARPQGESG-IGFVGEVASVNPQLIHNLLDGGHIPIVSSVATDEEGQVYNINADTV 186

Query: 250 AGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPKVNC 309
           AGELAAAL AEKLILLTD  GIL+D  D  SL+ ++DI+  ++++    + GGMIPKV C
Sbjct: 187 AGELAAALDAEKLILLTDTPGILQDYKDATSLIHKLDIRQARELIAAEVVAGGMIPKVTC 246

Query: 310 CVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMI 347
           C+RSLAQG++ A IIDGR  H+LLLEI TD+G GTMI
Sbjct: 247 CIRSLAQGIQAAHIIDGRAPHALLLEIFTDSGIGTMI 282

BLAST of Carg12173 vs. ExPASy Swiss-Prot
Match: B0JHB1 (Acetylglutamate kinase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=argB PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 5.1e-97
Identity = 174/278 (62.59%), Postives = 229/278 (82.37%), Query Frame = 0

Query: 70  RVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHGGGP 129
           RV +LSE+LP+IQ+FR +T+VVKYGGAAM +  ++ +V+ D+V LSCVG+RP++VHGGGP
Sbjct: 12  RVKILSEALPYIQEFRGRTVVVKYGGAAMNNSDIKDTVMRDIVFLSCVGVRPVIVHGGGP 71

Query: 130 EINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGASAAGLCGVD 189
           EIN+WL +L IE  F DGLRVTDA+TM +V MVLVG VNK +V+LIN+AG  A GLCG D
Sbjct: 72  EINSWLDKLGIEPQFKDGLRVTDADTMDVVEMVLVGRVNKEIVALINQAGGKAVGLCGKD 131

Query: 190 GRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINADTV 249
           G L+TARP      +GFVGEV+ VD +V+++L++ G+IPV+SSVAADE+GQ +NINADTV
Sbjct: 132 GNLITARPVGKEG-IGFVGEVSSVDTSVVESLVNSGYIPVISSVAADETGQAHNINADTV 191

Query: 250 AGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPKVNC 309
           AGE+AAALGA KLIL+TD AGIL++  DP +L+ ++DI+  ++++++G + GGMIPKV C
Sbjct: 192 AGEIAAALGAAKLILMTDTAGILQNYKDPSTLLAKLDIQQARELIQKGIVAGGMIPKVGC 251

Query: 310 CVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMII 348
           CVRSLAQGV+ A IIDGRL H+LLLEI TD G G+MI+
Sbjct: 252 CVRSLAQGVQAAHIIDGRLPHALLLEIFTDRGIGSMIV 288

BLAST of Carg12173 vs. ExPASy TrEMBL
Match: A0A6J1F2V3 (acetylglutamate kinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111439296 PE=3 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 9.2e-182
Identity = 344/348 (98.85%), Postives = 345/348 (99.14%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRR+SSSSSLSIRCSATQQMEAV
Sbjct: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRLSSSSSLSIRCSATQQMEAV 60

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
           VDSVSPEQFRVDVLSESLPFIQKFR KTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR
Sbjct: 61  VDSVSPEQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
           PILVHGGGPEINNWLKRLNIEVVF DGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA
Sbjct: 121 PILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIP VSSVAADESGQ
Sbjct: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPAVSSVAADESGQ 240

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
           MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG
Sbjct: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Sbjct: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of Carg12173 vs. ExPASy TrEMBL
Match: A0A6J1KMY4 (acetylglutamate kinase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495616 PE=3 SV=1)

HSP 1 Score: 639.4 bits (1648), Expect = 8.6e-180
Identity = 342/348 (98.28%), Postives = 342/348 (98.28%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           MP IESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISS SSLSIRCSAT QMEAV
Sbjct: 1   MPKIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSPSSLSIRCSATHQMEAV 60

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
           VDSVSPEQFRVDVLSESLPFIQKFR KTIVVKYGGAAMKS SLQASVVNDLVLLSCVGLR
Sbjct: 61  VDSVSPEQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSLSLQASVVNDLVLLSCVGLR 120

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
           PILVHGGGPEINNWLKRLNIEVVF DGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA
Sbjct: 121 PILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ
Sbjct: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
           MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG
Sbjct: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Sbjct: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of Carg12173 vs. ExPASy TrEMBL
Match: A0A6J1EUQ8 (acetylglutamate kinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111436114 PE=3 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 8.0e-178
Identity = 338/348 (97.13%), Postives = 342/348 (98.28%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           M T+ESLLNPSA  LFSSKSLFNPSISSSSIFSPAPRLRR+SSSSSLSIRCSATQQMEAV
Sbjct: 1   MSTVESLLNPSASKLFSSKSLFNPSISSSSIFSPAPRLRRLSSSSSLSIRCSATQQMEAV 60

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
           VDSVSP QFRVDVLSESLPFIQKFR KTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR
Sbjct: 61  VDSVSPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
           PILVHGGGPEINNWLKRLNIEVVF DGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA
Sbjct: 121 PILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ
Sbjct: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
           MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMM+EGRIG
Sbjct: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMDEGRIG 300

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKV+CCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Sbjct: 301 GGMIPKVSCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of Carg12173 vs. ExPASy TrEMBL
Match: A0A6J1K509 (acetylglutamate kinase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111492351 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 4.0e-177
Identity = 337/348 (96.84%), Postives = 341/348 (97.99%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           M T+ESLLNPSA  LFS KSLFNPSISSSSIFSPAPRLRR+SSSSSLSIRCSATQQMEAV
Sbjct: 1   MSTVESLLNPSASKLFSFKSLFNPSISSSSIFSPAPRLRRLSSSSSLSIRCSATQQMEAV 60

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
           VDSVSP QFRVDVLSESLPFIQKFR KTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR
Sbjct: 61  VDSVSPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
           PILVHGGGPEINNWLKRLNIEVVF DGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA
Sbjct: 121 PILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ
Sbjct: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
           MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMM+EGRIG
Sbjct: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMDEGRIG 300

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKV+CCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Sbjct: 301 GGMIPKVSCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 348

BLAST of Carg12173 vs. ExPASy TrEMBL
Match: A0A0A0LM56 (AA_kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379190 PE=3 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 5.4e-174
Identity = 333/351 (94.87%), Postives = 339/351 (96.58%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRI---SSSSSLSIRCSATQQM 60
           MP +ESLL+PSA  LF+SKSL NPSISSSSIFSPAPR RR+   SSSSSLSIRCSATQQM
Sbjct: 1   MPAVESLLSPSASKLFTSKSLLNPSISSSSIFSPAPRFRRLPSSSSSSSLSIRCSATQQM 60

Query: 61  EAVVDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCV 120
           EAVVDSV+P QFRVDVLSESLPFIQKFR KTIVVKYGGAAMKSRSLQASVVNDLVLLSCV
Sbjct: 61  EAVVDSVTPGQFRVDVLSESLPFIQKFRGKTIVVKYGGAAMKSRSLQASVVNDLVLLSCV 120

Query: 121 GLRPILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINK 180
           GLRPILVHGGGPEINNWLKRLNIEVVF DGLRVTDAETMKIVSMVLVGDVNKNLVSLINK
Sbjct: 121 GLRPILVHGGGPEINNWLKRLNIEVVFRDGLRVTDAETMKIVSMVLVGDVNKNLVSLINK 180

Query: 181 AGASAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE 240
           AGASAAGLCGVDGRLL ARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE
Sbjct: 181 AGASAAGLCGVDGRLLMARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADE 240

Query: 241 SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEG 300
           SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMM+EG
Sbjct: 241 SGQMYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMDEG 300

Query: 301 RIGGGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           RIGGGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG
Sbjct: 301 RIGGGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 351

BLAST of Carg12173 vs. TAIR 10
Match: AT3G57560.1 (N-acetyl-l-glutamate kinase )

HSP 1 Score: 432.6 bits (1111), Expect = 3.1e-121
Identity = 233/348 (66.95%), Postives = 278/348 (79.89%), Query Frame = 0

Query: 1   MPTIESLLNPSAFNLFSSKSLFNPSISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAV 60
           M T+ S  +P +F+ F+  + F   I + S     P   +       SI+ + +      
Sbjct: 1   MATVTSNASPKSFS-FTVSNPFKTLIPNKSPSLCYPTRNKNHHRLGFSIKATVSTPPSIA 60

Query: 61  VDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLR 120
             +     +RV++LSESLPFIQKFR KTIVVKYGGAAM S  L++SVV+DLVLL+CVGLR
Sbjct: 61  TGNAPSPDYRVEILSESLPFIQKFRGKTIVVKYGGAAMTSPELKSSVVSDLVLLACVGLR 120

Query: 121 PILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMKIVSMVLVGDVNKNLVSLINKAGA 180
           PILVHGGGP+IN +LK+LNI   F DGLRVTDA TM+IVSMVLVG VNKNLVSLIN AGA
Sbjct: 121 PILVHGGGPDINRYLKQLNIPAEFRDGLRVTDATTMEIVSMVLVGKVNKNLVSLINAAGA 180

Query: 181 SAAGLCGVDGRLLTARPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVAADESGQ 240
           +A GL G DGRLLTARP PN+AQLGFVGEVARVDP+VL+ L+D G+IPV++SVAAD+SGQ
Sbjct: 181 TAVGLSGHDGRLLTARPVPNSAQLGFVGEVARVDPSVLRPLVDYGYIPVIASVAADDSGQ 240

Query: 241 MYNINADTVAGELAAALGAEKLILLTDVAGILEDRDDPKSLVKQIDIKGVKKMMEEGRIG 300
            YNINADTVAGELAAALGAEKLILLTDVAGILE+++DP SL+K+IDIKGVKKM+E+G++ 
Sbjct: 241 AYNINADTVAGELAAALGAEKLILLTDVAGILENKEDPSSLIKEIDIKGVKKMIEDGKVA 300

Query: 301 GGMIPKVNCCVRSLAQGVRTASIIDGRLEHSLLLEIMTDNGAGTMIIG 349
           GGMIPKV CC+RSLAQGV+TASIIDGR +HSLL EIM+D GAGTMI G
Sbjct: 301 GGMIPKVKCCIRSLAQGVKTASIIDGRRQHSLLHEIMSDEGAGTMITG 347

BLAST of Carg12173 vs. TAIR 10
Match: AT4G37670.2 (N-acetyl-l-glutamate synthase 2 )

HSP 1 Score: 53.1 bits (126), Expect = 5.1e-07
Identity = 70/273 (25.64%), Postives = 119/273 (43.59%), Query Frame = 0

Query: 11  SAFNLFSSKSLFNPS--ISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAVVDSVSPEQ 70
           S F+ F  K+  NP+    + S F P   +   +  +      +A   +EA    V  +Q
Sbjct: 26  SNFSSFKPKNKLNPTQFRFNCSWFKPVSSI-TAAKCNMFDYAVTAAGDVEA-EHPVDDKQ 85

Query: 71  FRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHGGG 130
           F V    E+ P++   R  T VV   G  +   S  A ++ D+  L  +G+R +LV G  
Sbjct: 86  F-VRWFREAWPYLWAHRGCTFVVIISGEIIAGSSCDA-ILKDIAFLHHLGIRFVLVPGTQ 145

Query: 131 PEINNWLKRLNIEVVFCDGLRVTDAETMK-------IVSMVLVGDVN--KNLVSLINKAG 190
            +I+  L     E  +    RVTDA +++        +S++L   ++   ++ ++     
Sbjct: 146 EQIDQLLAERGREATYVGRYRVTDAASLQAAKEAAGAISVMLEAKLSPGPSICNIRRHGD 205

Query: 191 ASAAGLCGV---DGRLLTA--RPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVA 250
            S     GV    G    A  R   +    G  GEV ++D   +   LD G + ++ ++ 
Sbjct: 206 RSRLHDIGVRVDTGNFFAAKRRGVVDGVDFGATGEVKKIDVDRICERLDGGSVVLLRNLG 265

Query: 251 ADESGQMYNINADTVAGELAAALGAEKLILLTD 268
              SG++ N N   VA   A A+GA+KLI + D
Sbjct: 266 HSSSGEVLNCNTYEVATACALAIGADKLICIMD 294

BLAST of Carg12173 vs. TAIR 10
Match: AT4G37670.1 (N-acetyl-l-glutamate synthase 2 )

HSP 1 Score: 53.1 bits (126), Expect = 5.1e-07
Identity = 70/273 (25.64%), Postives = 119/273 (43.59%), Query Frame = 0

Query: 11  SAFNLFSSKSLFNPS--ISSSSIFSPAPRLRRISSSSSLSIRCSATQQMEAVVDSVSPEQ 70
           S F+ F  K+  NP+    + S F P   +   +  +      +A   +EA    V  +Q
Sbjct: 26  SNFSSFKPKNKLNPTQFRFNCSWFKPVSSI-TAAKCNMFDYAVTAAGDVEA-EHPVDDKQ 85

Query: 71  FRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRSLQASVVNDLVLLSCVGLRPILVHGGG 130
           F V    E+ P++   R  T VV   G  +   S  A ++ D+  L  +G+R +LV G  
Sbjct: 86  F-VRWFREAWPYLWAHRGCTFVVIISGEIIAGSSCDA-ILKDIAFLHHLGIRFVLVPGTQ 145

Query: 131 PEINNWLKRLNIEVVFCDGLRVTDAETMK-------IVSMVLVGDVN--KNLVSLINKAG 190
            +I+  L     E  +    RVTDA +++        +S++L   ++   ++ ++     
Sbjct: 146 EQIDQLLAERGREATYVGRYRVTDAASLQAAKEAAGAISVMLEAKLSPGPSICNIRRHGD 205

Query: 191 ASAAGLCGV---DGRLLTA--RPAPNAAQLGFVGEVARVDPTVLQALLDKGHIPVVSSVA 250
            S     GV    G    A  R   +    G  GEV ++D   +   LD G + ++ ++ 
Sbjct: 206 RSRLHDIGVRVDTGNFFAAKRRGVVDGVDFGATGEVKKIDVDRICERLDGGSVVLLRNLG 265

Query: 251 ADESGQMYNINADTVAGELAAALGAEKLILLTD 268
              SG++ N N   VA   A A+GA+KLI + D
Sbjct: 266 HSSSGEVLNCNTYEVATACALAIGADKLICIMD 294

BLAST of Carg12173 vs. TAIR 10
Match: AT2G22910.1 (N-acetyl-l-glutamate synthase 1 )

HSP 1 Score: 50.8 bits (120), Expect = 2.5e-06
Identity = 63/279 (22.58%), Postives = 119/279 (42.65%), Query Frame = 0

Query: 43  SSSSLSIRCSATQQMEAVVDSVSPEQFRVDVLSESLPFIQKFRRKTIVVKYGGAAMKSRS 102
           S +  + +C+     E   + V  +QF V    E+ P++   R  T VV   G  +    
Sbjct: 51  SPALTAAKCNIFDYAETGENLVGDKQF-VRWFREAWPYLWAHRSCTFVVTISGDVLDGPY 110

Query: 103 LQASVVNDLVLLSCVGLRPILVHGGGPEINNWLKRLNIEVVFCDGLRVTDAETMK----- 162
               V+ D+  L  +G++ +LV G   +I+  L     E  +    RVTD+ +++     
Sbjct: 111 CDL-VLKDIAFLHHLGIKFVLVPGTQVQIDQLLAERGREPTYVGRYRVTDSASLQAAKEA 170

Query: 163 --IVSMVLVGDVNKN-LVSLINKAGASA----AGLCGVDGRLLTA--RPAPNAAQLGFVG 222
              +S+++   ++    +  I + G S+     G+    G    A  R   +    G  G
Sbjct: 171 AGAISVMIEAKLSPGPSIYNIRRHGDSSRLHETGVRVDTGNFFAAKRRGVVDGVDFGATG 230

Query: 223 EVARVDPTVLQALLDKGHIPVVSSVAADESGQMYNINADTVAGELAAALGAEKLILLTDV 282
            V ++D   ++  LD G + ++ ++    +G++ N N   VA   A A+GA+KLI + D 
Sbjct: 231 LVKKIDVDRIRERLDSGSVVLLRNLGHSSTGEVLNCNTYEVATACALAIGADKLICIMDG 290

Query: 283 AGILEDRDDPKSLVKQIDIKGVKKMMEEGRIGGGMIPKV 308
             + E+    + L  Q     V+K  ++  I    +  V
Sbjct: 291 PVLDENGHLVRFLTLQEADTLVRKRAQQSEIAANYVKAV 327

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021826.12.4e-184100.00Acetylglutamate kinase, chloroplastic, partial [Cucurbita argyrosperma subsp. ar... [more]
XP_022932833.11.9e-18198.85acetylglutamate kinase, chloroplastic-like [Cucurbita moschata][more]
XP_023531202.13.2e-18199.14acetylglutamate kinase, chloroplastic-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
KAG6587937.16.1e-18098.28Acetylglutamate kinase, chloroplastic, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023001499.11.8e-17998.28acetylglutamate kinase, chloroplastic-like [Cucurbita maxima] >XP_023001506.1 ac... [more]
Match NameE-valueIdentityDescription
Q9SCL74.3e-12066.95Acetylglutamate kinase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NAGK PE... [more]
Q8YXG89.4e-9965.60Acetylglutamate kinase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) O... [more]
Q3MFQ82.1e-9865.60Acetylglutamate kinase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) O... [more]
B0CAM31.8e-9765.34Acetylglutamate kinase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=... [more]
B0JHB15.1e-9762.59Acetylglutamate kinase OS=Microcystis aeruginosa (strain NIES-843) OX=449447 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1F2V39.2e-18298.85acetylglutamate kinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KMY48.6e-18098.28acetylglutamate kinase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1EUQ88.0e-17897.13acetylglutamate kinase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1K5094.0e-17796.84acetylglutamate kinase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0LM565.4e-17494.87AA_kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G379190 P... [more]
Match NameE-valueIdentityDescription
AT3G57560.13.1e-12166.95N-acetyl-l-glutamate kinase [more]
AT4G37670.25.1e-0725.64N-acetyl-l-glutamate synthase 2 [more]
AT4G37670.15.1e-0725.64N-acetyl-l-glutamate synthase 2 [more]
AT2G22910.12.5e-0622.58N-acetyl-l-glutamate synthase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001057Glutamate/acetylglutamate kinasePRINTSPR00474GLU5KINASEcoord: 245..272
score: 36.51
coord: 298..318
score: 29.1
coord: 118..132
score: 29.63
IPR001048Aspartate/glutamate/uridylate kinasePFAMPF00696AA_kinasecoord: 87..324
e-value: 1.4E-43
score: 149.3
IPR036393Acetylglutamate kinase-like superfamilyGENE3D3.40.1160.10coord: 63..348
e-value: 3.1E-103
score: 346.8
IPR036393Acetylglutamate kinase-like superfamilySUPERFAMILY53633Carbamate kinase-likecoord: 70..347
IPR004662Acetylglutamate kinase familyTIGRFAMTIGR00761TIGR00761coord: 88..323
e-value: 1.4E-84
score: 281.2
NoneNo IPR availablePIRSRPIRSR016496-1PIRSR016496-1coord: 208..346
e-value: 2.0E-11
score: 41.1
NoneNo IPR availablePANTHERPTHR23342:SF16BNAC04G55460D PROTEINcoord: 2..348
NoneNo IPR availablePANTHERPTHR23342N-ACETYLGLUTAMATE SYNTHASEcoord: 2..348
IPR037528Acetylglutamate kinase ArgBHAMAPMF_00082ArgBcoord: 86..341
score: 48.138672
IPR041727N-Acetyl-L-glutamate kinase, cyclicCDDcd04250AAK_NAGK-Ccoord: 73..346
e-value: 6.98346E-146
score: 411.129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg12173-RACarg12173-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006526 arginine biosynthetic process
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016310 phosphorylation
cellular_component GO:0005759 mitochondrial matrix
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003991 acetylglutamate kinase activity
molecular_function GO:0034618 arginine binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity