Carg12107 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12107
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Thiamine pyrophosphokinase
Location	Carg_Chr05: 9962720 .. 9966665 (-)
RNA-Seq Expression	Carg12107
Synteny	Carg12107

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCAAATCTTCGTTCGGAGAGCCTTTCAATCGCGCTCATTATCGCCATGGGCGTCTTCTTCCACTCCTCAGATTTTCTCCTCCCTTCTTCTACTTCTTCTCCGGCCGGCCACCCACCTTCTTCGACCTACGCCCTTGTTCTGCTCAACCAAAGCCTCCCCAAATTCACTCCTCTGCTCTGGAAGCACGGTTACTCTTCAAATCCCTTTTGGCTTCATCTTTTGGTTCTAGGTTTTGATGATTTTTGCGTTTCGTGTTGATGCGTTCTTATGTGGGTTGTTGTAGAGCAGCAAAACTTCGTCTCTGTGCCGATGGAGGCGCCAATCGCGTATTTGATGAATTGCCTCTGCTGTTTCCTCATCAAGACGCTACGGATATTCGAAATAGGTTCGTTTCGTTGCGATTTTCTTCTTTTTCTTATCGTTTTTAGCGTGCATTTATGCCATTATCAGTGTTTTTCTTTTCTTTTTCTTTTCTTGTTGTGATTTAGCTTCTCTATTTAGTAATGGAGTTTCGCGCATAGGGGTTTTGTTGTTTTCTGGTAATTTAGATGGTTAATGCGTGAAAACATAAATCTTCTCCTTGGATGTTACTTCACAGTTTTATTTACCATTGACTGTTATGACGAACTCTTAGGGTTTATTATGGCCCCTTTCGTTACGAATTTGAATTATGTTTTATATTTTTTTGAAAACTGTTTTGGGATTCTTTGATTCAAATTGTGAATATTCTAAAATTTTTTTAACGTTTTCAATGTAGAATTTGGAATCTTAAAATGAAGAATTATATACTATTATAGTCTGATGATTAGTTTTAATTTGGTACTTTTAGGTGTTTTTTTTTTTTTTTTTTTTTTTGGATGCCAATTCAATGCCTCCAAGAAATGGGTTGAGTAAATGTTCTTTGAAAGTCGTTTTTCTTATTTCGGTTAGTGTTTTCTTTACTATTTTGGAAAATTTTCTTTCTTTAATTATCATGGACTTGTGGTTAGTGTTCTTATGTGCAAAGTAATCTTAATTTTTACAAATTACTCGACAATTCTGAAGAATAATCTTCTTTTCACAAAAAAAGAAAAAGAAACACTGACCCCAAGTTTCAAGATGTGATTGATACTAACAAAACAAATACTAGTGGCTAGCTAAAGAAGAAAATTTTGTTGCCATTGATGTCTTCATAATCATTTTCGAGCATTTATTGATAGTTTATACTATTGTTGTACAATGAATAAACTGTTCTAGAATTATATATCTTATGTTTTAAGTGATCTGGACTGTTCATTCATTCCTATCTATTGATTTTTTTTTCTTTTTTCTATTGCCAGCTATAAGCCAGATGTTATTAGAGGAGATATGGATTCAATTCGGACAGAAGTACTAGATTTTTATGCTAAGCAAGTACGTTACTAAGCCACCCCCTCCCTTCGAAGAATGTGGGGCTTTTTTAAATGATTTTTCAAGTGTTTGATACCATGGATGGTTCCATCTTGTGCATTTGATACCATGGATGGTTCCATCTTGTGCATTTGAGACCATGGATGGTTCCATCTTGTGCGTAATTTTTCCATTCTATGTTTTGATAAAGTTCACTTGCATTACATGTTGTATTTTACTATGCTGTTATCATGTATGGATTGCGGGCTCAAGATATCCATATTCACATGGTGCAGGGAACCAAGATATTTGATGAATCTGAAGATCAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAATCTATGCCAAATCAGGAGGAGTCTAATGTAGGTTGGATCATGATGTAAATCTTAAGTGGCTTGTTTATTAAACTAGAAGTTTTGGATGTTCGCATTCAACTACTGCGTTTTGTTTTGTGCGCTGACTTTTCAGATTGGGATTATAATGTTTGAGTTGTTGATTTTCAGCTGTGCATACTTGTTGCTGGAGCTCTAGGTGGACGGTTTGATCATGAGATGGGAAACATCAATGTTCTATGCCGTTTCTCTACCACGCGCATAATCCTTCTGTCTGATGATTGTCTTATTCATCTTCTGCCAAGGACTCATCACCATGAAATCCATGTTCACTCATCTGTTGAGGGCCCTCATTGTGGTCTCATTCCAATTGGGATGCCCTCTGGACGCACTACGACCACTGGTCTCCAATGGGATTTGAGTAAGTACTGTCCTCATTCCTCTTTCTGGATCTAATGCTCTGTTTATTTTCAAAGAATGAACGACTGCGTTGTGTTATATGTTCTTATGTGCATCCTATCCTGTTTATGCTTGGTTTTAAGCAATTTTGAGTTGGAAAATGAGCTGACAATGTGATCCATCAGTTTTATTCCTCTTGCTATGAGTTCATTCTTAACTTCATGTTTCAAAAAAAAACTTTGCAAGGAATTGGTCAATGCCAGCAGCTTTATGCTCAATTTCTTAAGCTTTTTTAGGAATTCCAAAACAAACAACTTCAACATAATCTTTCTGTAACACCGATCCAAGCCCACCACTAGCAGATATTGTCTTCTTTGGGCTTTCCCTCAAGATTTTTAAAACGTCTATGTTAGGGAGTGGTTTCCACACCCTTATAAAGAATGCTTTGTTCTCCCCCCAACCGATATGGGATCTCACAATCCACCCTCTTTCAAGGCTCAGTGTCCTTGTTGACAATTGTTCCCTTTTCCAATCAATGTGGGACCCCCAATCCACCCCCTTTGGGACCCGGCGTCCTTACTGGTACACTGCCTTGTGTCTACCTTCTTTCGAGGCTCAGCATCTTCCTCGCACATCTGCTTCCCTTAAGGTTTTTAAAGAATGTTTCCTTTCGAGGCTCAGCATTTGTAACGGCCCAAGTCCACCGCTTCCCTTAATCTTTTTAAAACGCATCTGCTAGGAAGAGGTTTCCATTCCACACCCTTACAAAGAATGCTTTATTCTCCTTCCCAACCTTCATCTTGTGATATTTTGAGAAGGTACTGTGGAAATAATTCATGTCTAACCTCATGAAGATTTCTTTCCAGATAGTCATTCGTTACTTCATTCTCTCCCTATGTGATTGTACTTTGGCATGATATTCCTTCAAGTTGTATGCTTACAATATTAGGGGATGTTGTACTTTCTTTTTCGATTCGTACTCTGCTCTTCCAGCTCCTATTTGCTCTTTCTCGACTGTTTTAGGGAAGTTTTTTTTTAATATACTTCGGGCACGTTTGGGAATGATTTTGAAACGGCGACAAATCACTTTTGTCATGTCTTTTAGTTATTTTGGGCTGTCTTAAAATCACTCCGAACATATCCTTATGGAATTCTAACGTGTGCTGTTAACCATTTTGGTATGAATGTGTATTCCAGCTGATACAGAGATGAAGTTTGGGGGTCTAGTTAGCACTTCAAATATAGTGAAAGGAGAGAAAGTAACAGTGCAATCCGACTCCGATCTTCTCTGGACTCTCTCATTAAAAGGCAATGAAACAAGGCACTCTAGCACGTAGTGCCAAATTTAGCAATGCTTCCTTGCATTCTCAGCGTTAGGTAATGAAATCTTCTTTCTCCACCTAAAATAATAATAACTTCGAAGATCTACCCCTTGATCTTTTGTGCTTTTCTAGAGTTTGATTTTCAAGAAATTCATTTTCCATGTAATTTCATTTTTATGTTGATTAGGAAGTTGTATTTGCAATGCATAAAACAGATCAGTGCCATTATTTGTATGTTATTTGTTTTCGGTAACATTCTATACTCTAATAAATTTAGAGTTAAAAAGACAGCCACTCACCTTGTTGAGCGTTTAGGTATATATATTCCTTATTATGAGGTCAAAGTTTCGAATCTTTTCACGAAACCTTATTTTATAAGTAATATTTTGATTACTATATTCTTTTTAGAAGTAAAACATTATACTTCATCTTCATCAGTCTCATTTATTTGGATAACTAGAGGTTTTCAAGGAGTTGAGTTGGCATATA

mRNA sequence

TCAAATCTTCGTTCGGAGAGCCTTTCAATCGCGCTCATTATCGCCATGGGCGTCTTCTTCCACTCCTCAGATTTTCTCCTCCCTTCTTCTACTTCTTCTCCGGCCGGCCACCCACCTTCTTCGACCTACGCCCTTGTTCTGCTCAACCAAAGCCTCCCCAAATTCACTCCTCTGCTCTGGAAGCACGCAAAACTTCGTCTCTGTGCCGATGGAGGCGCCAATCGCGTATTTGATGAATTGCCTCTGCTGTTTCCTCATCAAGACGCTACGGATATTCGAAATAGCTATAAGCCAGATGTTATTAGAGGAGATATGGATTCAATTCGGACAGAAGTACTAGATTTTTATGCTAAGCAAGGAACCAAGATATTTGATGAATCTGAAGATCAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAATCTATGCCAAATCAGGAGGAGTCTAATCTGTGCATACTTGTTGCTGGAGCTCTAGGTGGACGGTTTGATCATGAGATGGGAAACATCAATGTTCTATGCCGTTTCTCTACCACGCGCATAATCCTTCTGTCTGATGATTGTCTTATTCATCTTCTGCCAAGGACTCATCACCATGAAATCCATGTTCACTCATCTGTTGAGGGCCCTCATTGTGGTCTCATTCCAATTGGGATGCCCTCTGGACGCACTACGACCACTGGTCTCCAATGGGATTTGACTGATACAGAGATGAAGTTTGGGGGTCTAGTTAGCACTTCAAATATAGTGAAAGGAGAGAAAGTAACAGTGCAATCCGACTCCGATCTTCTCTGGACTCTCTCATTAAAAGGCAATGAAACAAGGCACTCTAGCACGTAGTGCCAAATTTAGCAATGCTTCCTTGCATTCTCAGCGTTAGGTAATGAAATCTTCTTTCTCCACCTAAAATAATAATAACTTCGAAGATCTACCCCTTGATCTTTTGTGCTTTTCTAGAGTTTGATTTTCAAGAAATTCATTTTCCATGTAATTTCATTTTTATGTTGATTAGGAAGTTGTATTTGCAATGCATAAAACAGATCAGTGCCATTATTTGTATGTTATTTGTTTTCGGTAACATTCTATACTCTAATAAATTTAGAGTTAAAAAGACAGCCACTCACCTTGTTGAGCGTTTAGGTATATATATTCCTTATTATGAGGTCAAAGTTTCGAATCTTTTCACGAAACCTTATTTTATAAGTAATATTTTGATTACTATATTCTTTTTAGAAGTAAAACATTATACTTCATCTTCATCAGTCTCATTTATTTGGATAACTAGAGGTTTTCAAGGAGTTGAGTTGGCATATA

Coding sequence (CDS)

ATGGGCGTCTTCTTCCACTCCTCAGATTTTCTCCTCCCTTCTTCTACTTCTTCTCCGGCCGGCCACCCACCTTCTTCGACCTACGCCCTTGTTCTGCTCAACCAAAGCCTCCCCAAATTCACTCCTCTGCTCTGGAAGCACGCAAAACTTCGTCTCTGTGCCGATGGAGGCGCCAATCGCGTATTTGATGAATTGCCTCTGCTGTTTCCTCATCAAGACGCTACGGATATTCGAAATAGCTATAAGCCAGATGTTATTAGAGGAGATATGGATTCAATTCGGACAGAAGTACTAGATTTTTATGCTAAGCAAGGAACCAAGATATTTGATGAATCTGAAGATCAGGATACCACAGACCTACATAAATGTGTTGCATATATTCTTCAATCTATGCCAAATCAGGAGGAGTCTAATCTGTGCATACTTGTTGCTGGAGCTCTAGGTGGACGGTTTGATCATGAGATGGGAAACATCAATGTTCTATGCCGTTTCTCTACCACGCGCATAATCCTTCTGTCTGATGATTGTCTTATTCATCTTCTGCCAAGGACTCATCACCATGAAATCCATGTTCACTCATCTGTTGAGGGCCCTCATTGTGGTCTCATTCCAATTGGGATGCCCTCTGGACGCACTACGACCACTGGTCTCCAATGGGATTTGACTGATACAGAGATGAAGTTTGGGGGTCTAGTTAGCACTTCAAATATAGTGAAAGGAGAGAAAGTAACAGTGCAATCCGACTCCGATCTTCTCTGGACTCTCTCATTAAAAGGCAATGAAACAAGGCACTCTAGCACGTAG

Protein sequence

MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANRVFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDLHKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHLLPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKGEKVTVQSDSDLLWTLSLKGNETRHSST

Homology

[Close]

BLAST of Carg12107 vs. NCBI nr
Match: KAG7030224.1 (Thiamine pyrophosphokinase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 550.4 bits (1417), Expect = 8.3e-153
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR
Sbjct: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG
Sbjct: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240

Query: 241 EKVTVQSDSDLLWTLSLKGNETRHSST 268
           EKVTVQSDSDLLWTLSLKGNETRHSST
Sbjct: 241 EKVTVQSDSDLLWTLSLKGNETRHSST 267

[Close]

BLAST of Carg12107 vs. NCBI nr
Match: KAG6593191.1 (Thiamine pyrophosphokinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 544.3 bits (1401), Expect = 6.0e-151
Identity = 266/268 (99.25%), Postives = 267/268 (99.63%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR
Sbjct: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIV+G
Sbjct: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVEG 240

Query: 241 EKVTVQSDSDLLWTLSLKG-NETRHSST 268
           EKVTVQSDSDLLWTLSLKG NETRHSST
Sbjct: 241 EKVTVQSDSDLLWTLSLKGSNETRHSST 268

[Close]

BLAST of Carg12107 vs. NCBI nr
Match: XP_023515266.1 (thiamine pyrophosphokinase 1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 543.9 bits (1400), Expect = 7.8e-151
Identity = 262/267 (98.13%), Postives = 266/267 (99.63%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR
Sbjct: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           VFDELPLL PHQDATDIRNSYKPDVIRGDMDSIRTEVL+FYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLLLPHQDATDIRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGN+NVLCRFS+TRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNVNVLCRFSSTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG
Sbjct: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240

Query: 241 EKVTVQSDSDLLWTLSLKGNETRHSST 268
           EKVTVQSDSDLLWT+SLKGNETRHSST
Sbjct: 241 EKVTVQSDSDLLWTISLKGNETRHSST 267

[Close]

BLAST of Carg12107 vs. NCBI nr
Match: XP_022960072.1 (thiamine pyrophosphokinase 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 540.0 bits (1390), Expect = 1.1e-149
Identity = 263/267 (98.50%), Postives = 266/267 (99.63%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR
Sbjct: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPNQ ESNLCILVAGALGGRFDHEMGNINVLCRFS+TRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNQ-ESNLCILVAGALGGRFDHEMGNINVLCRFSSTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG
Sbjct: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240

Query: 241 EKVTVQSDSDLLWTLSLKGNETRHSST 268
           EKV+VQSDSDLLWT+SLKGNETRHSST
Sbjct: 241 EKVSVQSDSDLLWTISLKGNETRHSST 266

[Close]

BLAST of Carg12107 vs. NCBI nr
Match: XP_023004625.1 (thiamine pyrophosphokinase 1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 535.4 bits (1378), Expect = 2.8e-148
Identity = 258/263 (98.10%), Postives = 262/263 (99.62%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQ LPKFTPLLWKHAKLRLCADGGANR
Sbjct: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQRLPKFTPLLWKHAKLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFS+TRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSSTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTD+EMKFGGLVSTSNIVKG
Sbjct: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDSEMKFGGLVSTSNIVKG 240

Query: 241 EKVTVQSDSDLLWTLSLKGNETR 264
           EKVTVQSDSDLLWT+SLKGNET+
Sbjct: 241 EKVTVQSDSDLLWTISLKGNETK 263

[Close]

BLAST of Carg12107 vs. ExPASy Swiss-Prot
Match: B9DGU7 (Thiamine pyrophosphokinase 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=2 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 3.0e-105
Identity = 182/258 (70.54%), Postives = 212/258 (82.17%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           M V  HSS FLLP   +S       + YALV+LNQSLP+FTPLLW+HAKLRLCADGGANR
Sbjct: 4   MDVMIHSSSFLLPCDETS-----TGTRYALVVLNQSLPRFTPLLWEHAKLRLCADGGANR 63

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL FP++DA  IRN YKPDVI+GDMDSIR +VLDFY   GTK+ DES DQDTTDL
Sbjct: 64  IYDELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDL 123

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
            KC+ YI  S  NQE S L IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 124 DKCILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 183

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LP+TH HEIH+ SS+EGPHCGLIPIG PS +TTT+GLQWDL++TEM+FGGL+STSN+VK 
Sbjct: 184 LPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKE 243

Query: 241 EKVTVQSDSDLLWTLSLK 259
           EK+TV+SDSDLLWT+S+K
Sbjct: 244 EKITVESDSDLLWTISIK 256

[Close]

BLAST of Carg12107 vs. ExPASy Swiss-Prot
Match: F4IV16 (Thiamine pyrophosphokinase 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 1.7e-103
Identity = 178/258 (68.99%), Postives = 210/258 (81.40%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           M V  HSS FLLP   +        + YALV+LNQ+LP+FTPLLW+HAKLRLCADGGANR
Sbjct: 5   MDVMIHSSSFLLPCDETC------GTRYALVVLNQNLPRFTPLLWEHAKLRLCADGGANR 64

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL FPH+D   IRN YKPDVI+GDMDSIR +VLDFY   GTK+ DES DQDTTDL
Sbjct: 65  IYDELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHDQDTTDL 124

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
            KC++YI  S  NQE S L IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 125 DKCISYIRHSTLNQESSRLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 184

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LP+TH HEIH+HSS++GPHCGLIPIG PS  TTT+GL+WDL++TEM+FGGL+STSN+VK 
Sbjct: 185 LPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLISTSNLVKE 244

Query: 241 EKVTVQSDSDLLWTLSLK 259
           E +TV+SDSDLLWT+S+K
Sbjct: 245 EIITVESDSDLLWTISIK 256

[Close]

BLAST of Carg12107 vs. ExPASy Swiss-Prot
Match: Q5JK24 (Thiamine pyrophosphokinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK1 PE=2 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 8.2e-95
Identity = 168/258 (65.12%), Postives = 202/258 (78.29%), Query Frame = 0


Query: 6   HSSDFLLPSSTSSPAGHPP-----SSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 65
           HSS FL   +TSSP  HPP     S+ YA+V+LNQ LP+F PLLW  A+LR+CADGGANR
Sbjct: 8   HSSSFLRLPATSSP--HPPPADDASAAYAVVVLNQRLPRFAPLLWDRARLRVCADGGANR 67

Query: 66  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 125
           VFD +P L P +D   +R  YKPDVI+GDMDSIR EV ++Y+  G +I DES DQDTTDL
Sbjct: 68  VFDGMPELLPAEDPDQVRMRYKPDVIKGDMDSIRPEVKEYYSNLGAEIVDESHDQDTTDL 127

Query: 126 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 185
           HKCV++I ++ P  EESNL ILV GALGGRFDHEMGNINVL RFS  RI+LLSDDC I L
Sbjct: 128 HKCVSFITRNPPGSEESNLYILVLGALGGRFDHEMGNINVLYRFSNIRIVLLSDDCSIFL 187

Query: 186 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 245
           LP+TH HEIH+  S+EGPHCGLIP+G PS  TTTTGL+W+L +T M +GGL+STSNIV+ 
Sbjct: 188 LPKTHSHEIHIERSIEGPHCGLIPMGSPSASTTTTGLRWNLDNTSMSYGGLISTSNIVEE 247

Query: 246 EKVTVQSDSDLLWTLSLK 259
           E V + SDSDL+WT+SL+
Sbjct: 248 ETVRITSDSDLIWTISLR 263

[Close]

BLAST of Carg12107 vs. ExPASy Swiss-Prot
Match: Q60DX1 (Thiamine pyrophosphokinase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 343.2 bits (879), Expect = 2.6e-93
Identity = 165/256 (64.45%), Postives = 199/256 (77.73%), Query Frame = 0


Query: 6   HSSDFLLPSSTSSPAG-HPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANRVFDE 65
           HSS FLLPS +++ AG     + YAL++LNQ LP+F P LW  A++R+CADGGANRVFD 
Sbjct: 9   HSSAFLLPSPSAAAAGADADGAAYALLVLNQRLPRFAPRLWDRAQVRVCADGGANRVFDG 68

Query: 66  LPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDLHKCV 125
           +P LFP QD  ++R  YKPDVI+GD+DS+R EV ++Y+  GT+I DES DQDTTDLHKCV
Sbjct: 69  MPELFPGQDPDEVRRRYKPDVIKGDLDSVRPEVKEYYSNMGTQIVDESHDQDTTDLHKCV 128

Query: 126 AYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHLLPRT 185
           A+I ++     +SNLCI   GALGGRFDHEMGNINVL  F   RIILLSDDCLI LLPRT
Sbjct: 129 AFITENSAIPNKSNLCIFALGALGGRFDHEMGNINVLHLFPNNRIILLSDDCLIFLLPRT 188

Query: 186 HHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKGEK-- 245
           H H IH+  S+EGPHCGLIPIG PS  TTTTGLQW+L +T M FGGL+STSNIV+ E   
Sbjct: 189 HTHNIHIERSIEGPHCGLIPIGAPSATTTTTGLQWNLDNTSMSFGGLISTSNIVREESTV 248

Query: 246 VTVQSDSDLLWTLSLK 259
           VT+ SDSDL+WT+SL+
Sbjct: 249 VTITSDSDLIWTISLR 264

[Close]

BLAST of Carg12107 vs. ExPASy Swiss-Prot
Match: Q0JMW0 (Thiamine pyrophosphokinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 4.2e-91
Identity = 160/258 (62.02%), Postives = 200/258 (77.52%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           M V  HSS FLLP     PA + P+  YALV+LNQ+LP+F P LW HAKLR+CADGGANR
Sbjct: 9   MEVMVHSSTFLLP-KLHQPA-NSPAKKYALVVLNQNLPRFVPRLWTHAKLRICADGGANR 68

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           +FDE+  +    D    R  Y P++I GDMDSIR EV  FY+ QG+KI D+S +Q+TTDL
Sbjct: 69  IFDEMFQMTNDPDYESTRKRYIPEIIEGDMDSIRPEVKQFYSSQGSKISDKSHNQETTDL 128

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKC++ I +  P+ E++NLC+LV GALGGRFDHE  NIN+L  FS  RI+LLSDDCLI L
Sbjct: 129 HKCISRIHRCTPDHEKTNLCVLVTGALGGRFDHEAANINILYLFSDMRIVLLSDDCLIRL 188

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LP+TH HEI++ SSVEGPHCGL P+G PSG TTTTGL+W+L++ +M+FG ++STSNIV  
Sbjct: 189 LPKTHKHEIYIESSVEGPHCGLFPVGAPSGSTTTTGLKWNLSEAKMRFGSMISTSNIVHA 248

Query: 241 EKVTVQSDSDLLWTLSLK 259
           EKVTVQSD+DLLWT+SL+
Sbjct: 249 EKVTVQSDADLLWTISLR 264

[Close]

BLAST of Carg12107 vs. ExPASy TrEMBL
Match: A0A6J1H6C2 (Thiamine pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111460928 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 5.4e-150
Identity = 263/267 (98.50%), Postives = 266/267 (99.63%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR
Sbjct: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPNQ ESNLCILVAGALGGRFDHEMGNINVLCRFS+TRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNQ-ESNLCILVAGALGGRFDHEMGNINVLCRFSSTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG
Sbjct: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240

Query: 241 EKVTVQSDSDLLWTLSLKGNETRHSST 268
           EKV+VQSDSDLLWT+SLKGNETRHSST
Sbjct: 241 EKVSVQSDSDLLWTISLKGNETRHSST 266

[Close]

BLAST of Carg12107 vs. ExPASy TrEMBL
Match: A0A6J1KV43 (Thiamine pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111497866 PE=3 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 1.3e-148
Identity = 258/263 (98.10%), Postives = 262/263 (99.62%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQ LPKFTPLLWKHAKLRLCADGGANR
Sbjct: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQRLPKFTPLLWKHAKLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFS+TRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSSTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTD+EMKFGGLVSTSNIVKG
Sbjct: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDSEMKFGGLVSTSNIVKG 240

Query: 241 EKVTVQSDSDLLWTLSLKGNETR 264
           EKVTVQSDSDLLWT+SLKGNET+
Sbjct: 241 EKVTVQSDSDLLWTISLKGNETK 263

[Close]

BLAST of Carg12107 vs. ExPASy TrEMBL
Match: A0A1S3BSZ4 (Thiamine pyrophosphokinase OS=Cucumis melo OX=3656 GN=LOC103492826 PE=3 SV=1)

HSP 1 Score: 477.6 bits (1228), Expect = 3.3e-131
Identity = 232/258 (89.92%), Postives = 244/258 (94.57%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           M V FHSSDFLLPS +SSP  H  SSTYALVLLNQ LPKFTPLLWKHA+LRLCADGGANR
Sbjct: 1   MEVLFHSSDFLLPSPSSSPPNHRRSSTYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           VFD+LPL+FPH DA D+RNSYKPDVIRGDMDSIRTEVL+FYAKQGTKIFDESEDQDTTDL
Sbjct: 61  VFDDLPLMFPHLDALDVRNSYKPDVIRGDMDSIRTEVLEFYAKQGTKIFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQS+PNQ ESNLCILVAGALGGRFDHE+GNINVLCRFSTTRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSIPNQ-ESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LPRTHHHEI +HSSVEGPHCGLIPIGMPSG TTTTGLQWDL+DTEMKFGGL+STSNIVK 
Sbjct: 181 LPRTHHHEILIHSSVEGPHCGLIPIGMPSGSTTTTGLQWDLSDTEMKFGGLISTSNIVKE 240

Query: 241 EKVTVQSDSDLLWTLSLK 259
           EKVTVQSDSDLLWT+SLK
Sbjct: 241 EKVTVQSDSDLLWTISLK 257

[Close]

BLAST of Carg12107 vs. ExPASy TrEMBL
Match: A0A6J1GPJ2 (Thiamine pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111455967 PE=3 SV=1)

HSP 1 Score: 471.9 bits (1213), Expect = 1.8e-129
Identity = 227/258 (87.98%), Postives = 240/258 (93.02%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           M V FHSS+FLLPSS+S PA H  SSTYALVLLNQ LPKFTPLLWKHA+LRLCADGGANR
Sbjct: 1   MSVLFHSSEFLLPSSSSFPAEHRRSSTYALVLLNQRLPKFTPLLWKHAQLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           +FDELPLLFPH DA D+RNSYKPDVI GDMDSIRTEVLDFYAKQGTKIFDESED+DTTDL
Sbjct: 61  IFDELPLLFPHLDALDVRNSYKPDVISGDMDSIRTEVLDFYAKQGTKIFDESEDEDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMPN EESNLCILVAGALGGRFDHE+GNINVLCRFSTTRIILLSDDCL+HL
Sbjct: 121 HKCVAYILQSMPNLEESNLCILVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLVHL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LPRTHHHEIHV SSVEGPHCGLIPIGMPSG TTT GLQWDLTDT+ KFGGL+S+SN VKG
Sbjct: 181 LPRTHHHEIHVDSSVEGPHCGLIPIGMPSGSTTTNGLQWDLTDTKTKFGGLISSSNKVKG 240

Query: 241 EKVTVQSDSDLLWTLSLK 259
           EKV V SD+DLLWT+SLK
Sbjct: 241 EKVRVLSDTDLLWTISLK 258

[Close]

BLAST of Carg12107 vs. ExPASy TrEMBL
Match: A0A6J1D8K1 (Thiamine pyrophosphokinase OS=Momordica charantia OX=3673 GN=LOC111017915 PE=3 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 6.9e-129
Identity = 231/267 (86.52%), Postives = 245/267 (91.76%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           M   FHSSDFLLPSST  PA +  SSTYALVLLNQ LP+FTPLLWKHA+LRLCADGGANR
Sbjct: 1   MAAMFHSSDFLLPSST--PADNRRSSTYALVLLNQRLPRFTPLLWKHAQLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           V+DELP  FPH DA D+RNSYKPDVIRGDMDSIRTEVLDFYAK+GTK+FDESEDQDTTDL
Sbjct: 61  VYDELPSFFPHLDALDVRNSYKPDVIRGDMDSIRTEVLDFYAKKGTKVFDESEDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
           HKCVAYILQSMP+Q ESNLCI+VAGALGGRFDHE+GNINVLCRFSTTRIILLSDDCLIHL
Sbjct: 121 HKCVAYILQSMPSQ-ESNLCIVVAGALGGRFDHEIGNINVLCRFSTTRIILLSDDCLIHL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LP+T  HEI VHSSVEGPHCGLIPIGMPSG TTTTGL+WDLTDTEMKFGGL+STSNIVKG
Sbjct: 181 LPKTRAHEIQVHSSVEGPHCGLIPIGMPSGSTTTTGLKWDLTDTEMKFGGLISTSNIVKG 240

Query: 241 EKVTVQSDSDLLWTLSLKGNETRHSST 268
           EKVTVQSDSDLLWT+SLK   T HSST
Sbjct: 241 EKVTVQSDSDLLWTISLKSTGTAHSST 264

[Close]

BLAST of Carg12107 vs. TAIR 10
Match: AT1G02880.2 (thiamin pyrophosphokinase1 )

HSP 1 Score: 382.9 bits (982), Expect = 2.1e-106
Identity = 182/258 (70.54%), Postives = 212/258 (82.17%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           M V  HSS FLLP   +S       + YALV+LNQSLP+FTPLLW+HAKLRLCADGGANR
Sbjct: 1   MDVMIHSSSFLLPCDETS-----TGTRYALVVLNQSLPRFTPLLWEHAKLRLCADGGANR 60

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL FP++DA  IRN YKPDVI+GDMDSIR +VLDFY   GTK+ DES DQDTTDL
Sbjct: 61  IYDELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDL 120

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
            KC+ YI  S  NQE S L IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 121 DKCILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 180

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LP+TH HEIH+ SS+EGPHCGLIPIG PS +TTT+GLQWDL++TEM+FGGL+STSN+VK 
Sbjct: 181 LPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKE 240

Query: 241 EKVTVQSDSDLLWTLSLK 259
           EK+TV+SDSDLLWT+S+K
Sbjct: 241 EKITVESDSDLLWTISIK 253

[Close]

BLAST of Carg12107 vs. TAIR 10
Match: AT1G02880.3 (thiamin pyrophosphokinase1 )

HSP 1 Score: 382.9 bits (982), Expect = 2.1e-106
Identity = 182/258 (70.54%), Postives = 212/258 (82.17%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           M V  HSS FLLP   +S       + YALV+LNQSLP+FTPLLW+HAKLRLCADGGANR
Sbjct: 4   MDVMIHSSSFLLPCDETS-----TGTRYALVVLNQSLPRFTPLLWEHAKLRLCADGGANR 63

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL FP++DA  IRN YKPDVI+GDMDSIR +VLDFY   GTK+ DES DQDTTDL
Sbjct: 64  IYDELPLFFPNEDALAIRNRYKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDL 123

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
            KC+ YI  S  NQE S L IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 124 DKCILYIRHSTLNQETSGLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 183

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LP+TH HEIH+ SS+EGPHCGLIPIG PS +TTT+GLQWDL++TEM+FGGL+STSN+VK 
Sbjct: 184 LPKTHRHEIHIQSSLEGPHCGLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKE 243

Query: 241 EKVTVQSDSDLLWTLSLK 259
           EK+TV+SDSDLLWT+S+K
Sbjct: 244 EKITVESDSDLLWTISIK 256

[Close]

BLAST of Carg12107 vs. TAIR 10
Match: AT2G44750.2 (thiamin pyrophosphokinase 2 )

HSP 1 Score: 377.1 bits (967), Expect = 1.2e-104
Identity = 178/258 (68.99%), Postives = 210/258 (81.40%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           M V  HSS FLLP   +        + YALV+LNQ+LP+FTPLLW+HAKLRLCADGGANR
Sbjct: 5   MDVMIHSSSFLLPCDETC------GTRYALVVLNQNLPRFTPLLWEHAKLRLCADGGANR 64

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL FPH+D   IRN YKPDVI+GDMDSIR +VLDFY   GTK+ DES DQDTTDL
Sbjct: 65  IYDELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHDQDTTDL 124

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
            KC++YI  S  NQE S L IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 125 DKCISYIRHSTLNQESSRLQILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 184

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LP+TH HEIH+HSS++GPHCGLIPIG PS  TTT+GL+WDL++TEM+FGGL+STSN+VK 
Sbjct: 185 LPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLISTSNLVKE 244

Query: 241 EKVTVQSDSDLLWTLSLK 259
           E +TV+SDSDLLWT+S+K
Sbjct: 245 EIITVESDSDLLWTISIK 256

[Close]

BLAST of Carg12107 vs. TAIR 10
Match: AT2G44750.1 (thiamin pyrophosphokinase 2 )

HSP 1 Score: 371.7 bits (953), Expect = 4.9e-103
Identity = 177/258 (68.60%), Postives = 209/258 (81.01%), Query Frame = 0


Query: 1   MGVFFHSSDFLLPSSTSSPAGHPPSSTYALVLLNQSLPKFTPLLWKHAKLRLCADGGANR 60
           M V  HSS FLLP   +        + YALV+LNQ+LP+FTPLLW+HAKLRLCADGGANR
Sbjct: 5   MDVMIHSSSFLLPCDETC------GTRYALVVLNQNLPRFTPLLWEHAKLRLCADGGANR 64

Query: 61  VFDELPLLFPHQDATDIRNSYKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDL 120
           ++DELPL FPH+D   IRN YKPDVI+GDMDSIR +VLDFY   GTK+ DES DQDTTDL
Sbjct: 65  IYDELPLFFPHEDPFVIRNRYKPDVIKGDMDSIRRDVLDFYVYWGTKVIDESHDQDTTDL 124

Query: 121 HKCVAYILQSMPNQEESNLCILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHL 180
            KC++YI  S  NQE S   IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI L
Sbjct: 125 DKCISYIRHSTLNQESSR--ILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQL 184

Query: 181 LPRTHHHEIHVHSSVEGPHCGLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKG 240
           LP+TH HEIH+HSS++GPHCGLIPIG PS  TTT+GL+WDL++TEM+FGGL+STSN+VK 
Sbjct: 185 LPKTHRHEIHIHSSLQGPHCGLIPIGTPSANTTTSGLKWDLSNTEMRFGGLISTSNLVKE 244

Query: 241 EKVTVQSDSDLLWTLSLK 259
           E +TV+SDSDLLWT+S+K
Sbjct: 245 EIITVESDSDLLWTISIK 254

[Close]

BLAST of Carg12107 vs. TAIR 10
Match: AT1G02880.1 (thiamin pyrophosphokinase1 )

HSP 1 Score: 279.6 bits (714), Expect = 2.6e-75
Identity = 130/178 (73.03%), Postives = 151/178 (84.83%), Query Frame = 0


Query: 81  YKPDVIRGDMDSIRTEVLDFYAKQGTKIFDESEDQDTTDLHKCVAYILQSMPNQEESNLC 140
           YKPDVI+GDMDSIR +VLDFY   GTK+ DES DQDTTDL KC+ YI  S  NQE S L 
Sbjct: 9   YKPDVIKGDMDSIRRDVLDFYINLGTKVIDESHDQDTTDLDKCILYIRHSTLNQETSGLQ 68

Query: 141 ILVAGALGGRFDHEMGNINVLCRFSTTRIILLSDDCLIHLLPRTHHHEIHVHSSVEGPHC 200
           IL  GALGGRFDHE GN+NVL R+  TRI+LLSDDCLI LLP+TH HEIH+ SS+EGPHC
Sbjct: 69  ILATGALGGRFDHEAGNLNVLYRYPDTRIVLLSDDCLIQLLPKTHRHEIHIQSSLEGPHC 128

Query: 201 GLIPIGMPSGRTTTTGLQWDLTDTEMKFGGLVSTSNIVKGEKVTVQSDSDLLWTLSLK 259
           GLIPIG PS +TTT+GLQWDL++TEM+FGGL+STSN+VK EK+TV+SDSDLLWT+S+K
Sbjct: 129 GLIPIGTPSAKTTTSGLQWDLSNTEMRFGGLISTSNLVKEEKITVESDSDLLWTISIK 186

The following BLAST results are available for this feature:

BLAST of Carg12107 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030224.1	8.3e-153	100.00	Thiamine pyrophosphokinase 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6593191.1	6.0e-151	99.25	Thiamine pyrophosphokinase 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023515266.1	7.8e-151	98.13	thiamine pyrophosphokinase 1 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022960072.1	1.1e-149	98.50	thiamine pyrophosphokinase 1 isoform X1 [Cucurbita moschata]	[more]
XP_023004625.1	2.8e-148	98.10	thiamine pyrophosphokinase 1 isoform X1 [Cucurbita maxima]	[more]

BLAST of Carg12107 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9DGU7	3.0e-105	70.54	Thiamine pyrophosphokinase 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=2 SV=1	[more]
F4IV16	1.7e-103	68.99	Thiamine pyrophosphokinase 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1	[more]
Q5JK24	8.2e-95	65.12	Thiamine pyrophosphokinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK1 PE...	[more]
Q60DX1	2.6e-93	64.45	Thiamine pyrophosphokinase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK3 PE...	[more]
Q0JMW0	4.2e-91	62.02	Thiamine pyrophosphokinase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=TPK2 PE...	[more]

BLAST of Carg12107 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1H6C2	5.4e-150	98.50	Thiamine pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111460928 PE=3 SV...	[more]
A0A6J1KV43	1.3e-148	98.10	Thiamine pyrophosphokinase OS=Cucurbita maxima OX=3661 GN=LOC111497866 PE=3 SV=1	[more]
A0A1S3BSZ4	3.3e-131	89.92	Thiamine pyrophosphokinase OS=Cucumis melo OX=3656 GN=LOC103492826 PE=3 SV=1	[more]
A0A6J1GPJ2	1.8e-129	87.98	Thiamine pyrophosphokinase OS=Cucurbita moschata OX=3662 GN=LOC111455967 PE=3 SV...	[more]
A0A6J1D8K1	6.9e-129	86.52	Thiamine pyrophosphokinase OS=Momordica charantia OX=3673 GN=LOC111017915 PE=3 S...	[more]

BLAST of Carg12107 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02880.2	2.1e-106	70.54	thiamin pyrophosphokinase1	[more]
AT1G02880.3	2.1e-106	70.54	thiamin pyrophosphokinase1	[more]
AT2G44750.2	1.2e-104	68.99	thiamin pyrophosphokinase 2	[more]
AT2G44750.1	4.9e-103	68.60	thiamin pyrophosphokinase 2	[more]
AT1G02880.1	2.6e-75	73.03	thiamin pyrophosphokinase1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007373	Thiamin pyrophosphokinase, thiamin-binding domain	SMART	SM00983	TPK_B1_binding_a_2_a	coord: 185..251 e-value: 5.8E-12 score: 55.7
IPR007373	Thiamin pyrophosphokinase, thiamin-binding domain	PFAM	PF04265	TPK_B1_binding	coord: 186..252 e-value: 8.8E-23 score: 79.8
None	No IPR available	GENE3D	2.60.120.320		coord: 169..259 e-value: 3.5E-30 score: 105.5
None	No IPR available	PANTHER	PTHR13622	THIAMIN PYROPHOSPHOKINASE	coord: 4..258
None	No IPR available	PANTHER	PTHR13622:SF12	THIAMINE PYROPHOSPHOKINASE 1	coord: 4..258
IPR016966	Thiamin pyrophosphokinase, eukaryotic	PIRSF	PIRSF031057	TPK1	coord: 3..265 e-value: 7.4E-68 score: 226.5
IPR036759	Thiamin pyrophosphokinase, catalytic domain superfamily	GENE3D	3.40.50.10240	Thiamin pyrophosphokinase, catalytic domain	coord: 26..168 e-value: 1.4E-46 score: 160.4
IPR036759	Thiamin pyrophosphokinase, catalytic domain superfamily	SUPERFAMILY	63999	Thiamin pyrophosphokinase, catalytic domain	coord: 22..177
IPR006282	Thiamin pyrophosphokinase	TIGRFAM	TIGR01378	TIGR01378	coord: 30..254 e-value: 4.7E-61 score: 203.8
IPR007371	Thiamin pyrophosphokinase, catalytic domain	PFAM	PF04263	TPK_catalytic	coord: 43..169 e-value: 8.3E-42 score: 142.1
IPR007371	Thiamin pyrophosphokinase, catalytic domain	CDD	cd07995	TPK	coord: 29..256 e-value: 5.38217E-60 score: 187.367
IPR036371	Thiamin pyrophosphokinase, thiamin-binding domain superfamily	SUPERFAMILY	63862	Thiamin pyrophosphokinase, substrate-binding domain	coord: 179..260

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12107-RA	Carg12107-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006298	mismatch repair
biological_process	GO:0000398	mRNA splicing, via spliceosome
biological_process	GO:0000956	nuclear-transcribed mRNA catabolic process
biological_process	GO:0016310	phosphorylation
biological_process	GO:0009229	thiamine diphosphate biosynthetic process
biological_process	GO:0006772	thiamine metabolic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0032300	mismatch repair complex
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0016301	kinase activity
molecular_function	GO:0030983	mismatched DNA binding
molecular_function	GO:0030975	thiamine binding
molecular_function	GO:0004788	thiamine diphosphokinase activity