Carg12101 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg12101
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	pectinesterase inhibitor 6
Location	Carg_Chr05: 9938560 .. 9939153 (-)
RNA-Seq Expression	Carg12101
Synteny	Carg12101

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCGTGCACCCACTTCAAGAACCTCAATCGTCACCCTTTTAGCCTTCATTTCAATTCTTCCATGGCTGACACACTCAGCCCAAACGAGCTACGTCCAAGAAGCGTGCAGCGTAACACTACACCCAGATCTCTGCATCCGTTCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCTACAAAGTGGGCAAGGGCGAGCGTGTCGGTGACGATAACTGAGGCCAAAGCCGTGGCGAGGTTCTTGGTGAGGCTGAAGAACAACAGACGTATGAAGGGAAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGACGCCATTGATGAGCTTCACAGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAAATTTGACGCACAAATGGGGGATCTGACGACGTGGGTTAGCGCTGCTCTGACAGATGAAGATACTTGCGTGGATGGATTCGAAGGGGAAAGGGGAAGATTAGTGAATTTGATACGGAATCGTGTGGTGAAGGCTACTTATATTACCAGTAATGCGCTCGCTTTGGTTAACAAATTGGCTGCCTCTGGTTTTGAAACTGCCCCCAATCTATGA

mRNA sequence

ATGTTGCGTGCACCCACTTCAAGAACCTCAATCGTCACCCTTTTAGCCTTCATTTCAATTCTTCCATGGCTGACACACTCAGCCCAAACGAGCTACGTCCAAGAAGCGTGCAGCGTAACACTACACCCAGATCTCTGCATCCGTTCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCTACAAAGTGGGCAAGGGCGAGCGTGTCGGTGACGATAACTGAGGCCAAAGCCGTGGCGAGGTTCTTGGTGAGGCTGAAGAACAACAGACGTATGAAGGGAAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGACGCCATTGATGAGCTTCACAGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAAATTTGACGCACAAATGGGGGATCTGACGACGTGGGTTAGCGCTGCTCTGACAGATGAAGATACTTGCGTGGATGGATTCGAAGGGGAAAGGGGAAGATTAGTGAATTTGATACGGAATCGTGTGGTGAAGGCTACTTATATTACCAGTAATGCGCTCGCTTTGGTTAACAAATTGGCTGCCTCTGGTTTTGAAACTGCCCCCAATCTATGA

Coding sequence (CDS)

ATGTTGCGTGCACCCACTTCAAGAACCTCAATCGTCACCCTTTTAGCCTTCATTTCAATTCTTCCATGGCTGACACACTCAGCCCAAACGAGCTACGTCCAAGAAGCGTGCAGCGTAACACTACACCCAGATCTCTGCATCCGTTCGCTGTCGCCATTTTCAAGCACCGCCAAGCGAAGCCCTACAAAGTGGGCAAGGGCGAGCGTGTCGGTGACGATAACTGAGGCCAAAGCCGTGGCGAGGTTCTTGGTGAGGCTGAAGAACAACAGACGTATGAAGGGAAGAAACAGGGCTGCAGTTTCGGACTGTGTGGAGGTCTTTGAAGACGCCATTGATGAGCTTCACAGGTCACTTGGGGTTCTTAGAAGACTGAGTAGGAGCAAATTTGACGCACAAATGGGGGATCTGACGACGTGGGTTAGCGCTGCTCTGACAGATGAAGATACTTGCGTGGATGGATTCGAAGGGGAAAGGGGAAGATTAGTGAATTTGATACGGAATCGTGTGGTGAAGGCTACTTATATTACCAGTAATGCGCTCGCTTTGGTTAACAAATTGGCTGCCTCTGGTTTTGAAACTGCCCCCAATCTATGA

Protein sequence

MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAASGFETAPNL

Homology

[Close]

BLAST of Carg12101 vs. NCBI nr
Match: XP_023514751.1 (pectinesterase inhibitor 6 [Cucurbita pepo subsp. pepo] >KAG7030218.1 Pectinesterase inhibitor 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 379.4 bits (973), Expect = 1.9e-101
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAASGFETAPNL
Sbjct: 181 ALVNKLAASGFETAPNL 197

[Close]

BLAST of Carg12101 vs. NCBI nr
Match: KAG6593199.1 (Pectinesterase inhibitor 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 378.3 bits (970), Expect = 4.2e-101
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGFETAPN 197
           ALVNKLAASGFETAPN
Sbjct: 181 ALVNKLAASGFETAPN 196

[Close]

BLAST of Carg12101 vs. NCBI nr
Match: XP_023004663.1 (pectinesterase inhibitor 6 [Cucurbita maxima])

HSP 1 Score: 378.3 bits (970), Expect = 4.2e-101
Identity = 196/197 (99.49%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAPTSRTSIVTLLAFI+ILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFIAILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAASGFETAPNL
Sbjct: 181 ALVNKLAASGFETAPNL 197

[Close]

BLAST of Carg12101 vs. NCBI nr
Match: XP_022960074.1 (pectinesterase inhibitor 6-like [Cucurbita moschata])

HSP 1 Score: 376.3 bits (965), Expect = 1.6e-100
Identity = 195/197 (98.98%), Postives = 195/197 (98.98%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS
Sbjct: 63  MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 122

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV
Sbjct: 123 PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 182

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNR VKATYITSNAL
Sbjct: 183 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRAVKATYITSNAL 242

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAASGFET PNL
Sbjct: 243 ALVNKLAASGFETGPNL 259

[Close]

BLAST of Carg12101 vs. NCBI nr
Match: XP_038896715.1 (pectinesterase inhibitor 6-like [Benincasa hispida])

HSP 1 Score: 318.9 bits (816), Expect = 3.0e-83
Identity = 166/196 (84.69%), Postives = 176/196 (89.80%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAP +R SI T LA ISILP LTHS +TSYV+EACSVT H DLCI+SL+PFSSTAKRS
Sbjct: 1   MLRAPNTRASIATFLALISILPRLTHSTKTSYVREACSVTRHQDLCIQSLTPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           P KWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAVSDCVEVFE AIDELHRSLGV
Sbjct: 61  PAKWARAGVSVTITEAKTVAGLLGRLKNNKRMKGRNRAAVSDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSRS FDAQ+GDLTTWVSAALTDEDTCV+GFEGERG+LVNLIRNRV+K  YITSNAL
Sbjct: 121 LRRLSRSNFDAQIGDLTTWVSAALTDEDTCVEGFEGERGKLVNLIRNRVLKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPN 197
           ALVNKLAASG ETAPN
Sbjct: 181 ALVNKLAASGLETAPN 196

[Close]

BLAST of Carg12101 vs. ExPASy Swiss-Prot
Match: O22244 (Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.9e-36
Identity = 82/164 (50.00%), Postives = 113/164 (68.90%), Query Frame = 0


Query: 30  TSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNN 89
           ++YV+ AC+VT +  LC+R+L PF+  A+ + +KWARASV+VTIT+ K V R L  LK  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 90  RRMKGRN-RAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALTDED 149
           R   G + R A+SDC E+F D++D L++SL VLR L+  +F  Q+ DL TW+SAALTD+D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 150 TCVDGFE--GERGRLVNLIRNRVVKATYITSNALALVNKLAASG 191
           TC+DGFE    R R V ++R +  K   + SNALAL+ KLA  G
Sbjct: 166 TCLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLAFDG 207

[Close]

BLAST of Carg12101 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 4.1e-27
Identity = 67/184 (36.41%), Postives = 111/184 (60.33%), Query Frame = 0


Query: 10  SIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASV 69
           S +   A + IL   + +    +++ +C++T +P +C +SLS ++ T + +P + A  ++
Sbjct: 6   STLVFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTAL 65

Query: 70  SVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSK- 129
            V++T  +    F+ RL   + +K R  AA+ DC+E  ED++D + RS   ++ LS +K 
Sbjct: 66  QVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKG 125

Query: 130 --FDAQMGDLTTWVSAALTDEDTCVDGFEGE--RGRLVNLIRNRVVKATYITSNALALVN 189
             F  +M ++ TWVSAALTDE TC+DGF G+   G++   +R +VV    +TSNALALVN
Sbjct: 126 NDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVN 185

[Close]

BLAST of Carg12101 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.6e-26
Identity = 63/170 (37.06%), Postives = 108/170 (63.53%), Query Frame = 0


Query: 25  THSAQTS--YVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARF 84
           T+S QT+  Y++ +C++TL+  +C  SLSP++ST + +P K A  ++++T++ AK+ ++F
Sbjct: 138 TYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSASKF 197

Query: 85  LVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELH---RSLGVLRRLSRSKFDAQMGDLTTW 144
           +  + +   +      AV+DCVE   D++  L    R L  +     +KF+  M D+ TW
Sbjct: 198 VKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSDVETW 257

Query: 145 VSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNALALVNKLAAS 190
           VSAALT++DTC+DGF   +  + +L+R  VV+   +TSNALAL+N  A++
Sbjct: 258 VSAALTNDDTCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALINMYAST 307

[Close]

BLAST of Carg12101 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.0e-25
Identity = 69/193 (35.75%), Postives = 113/193 (58.55%), Query Frame = 0


Query: 4   APTSRTSIVTLLAFISILPWLT----HSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKR 63
           APT    +V  +AF  I    T    H+     V  +C    +P LC+R+LS +S     
Sbjct: 2   APTQNLFLVA-IAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTIT 61

Query: 64  SPTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLG 123
           +    A+A++ ++++ A++ A+ L  ++++   K + +AA+ DCVE+  D++DEL R+LG
Sbjct: 62  NRRDLAQAAIKISLSHAQSAAKKLAVVRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLG 121

Query: 124 VLRRL-----SRSKFDAQMGDLTTWVSAALTDEDTCVDGFEG-ERGRLVNLIRNRVVKAT 183
           VL+ L     S  +F  QM +  TW SAALTD+DTC+DGF+G + G +   ++  + K  
Sbjct: 122 VLKHLRVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMDDGEIKTEVKQWMTKVA 181

Query: 184 YITSNALALVNKL 187
            +TSNAL +VN+L
Sbjct: 182 RVTSNALYMVNQL 193

[Close]

BLAST of Carg12101 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.7e-23
Identity = 60/170 (35.29%), Postives = 98/170 (57.65%), Query Frame = 0


Query: 24  LTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFL 83
           +T     +++Q +C  T +P +C+ SL+ ++++ + SP + A  +++VT+T+A++   F+
Sbjct: 30  ITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSPRRLAETALNVTVTQAQSTKVFV 89

Query: 84  VRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRL----SRSKFDAQMGDLTTW 143
            RL     +K R   AV DC+E   DA+D L  S+  ++       R +F   M +  TW
Sbjct: 90  WRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEVKMCGSAKGRDQFWFHMSNAQTW 149

Query: 144 VSAALTDEDTCVDGFEGE--RGRLVNLIRNRVVKATYITSNALALVNKLA 188
            SAALT+ +TC DGF G    GR+ N +R R++     TSNALAL+N  A
Sbjct: 150 TSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRGTSNALALINAFA 199

[Close]

BLAST of Carg12101 vs. ExPASy TrEMBL
Match: A0A6J1L076 (pectinesterase inhibitor 6 OS=Cucurbita maxima OX=3661 GN=LOC111497889 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.0e-101
Identity = 196/197 (99.49%), Postives = 197/197 (100.00%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAPTSRTSIVTLLAFI+ILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS
Sbjct: 1   MLRAPTSRTSIVTLLAFIAILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV
Sbjct: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL
Sbjct: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAASGFETAPNL
Sbjct: 181 ALVNKLAASGFETAPNL 197

[Close]

BLAST of Carg12101 vs. ExPASy TrEMBL
Match: A0A6J1H9V5 (pectinesterase inhibitor 6-like OS=Cucurbita moschata OX=3662 GN=LOC111460929 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 7.7e-101
Identity = 195/197 (98.98%), Postives = 195/197 (98.98%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS
Sbjct: 63  MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 122

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV
Sbjct: 123 PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 182

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNR VKATYITSNAL
Sbjct: 183 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRAVKATYITSNAL 242

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAASGFET PNL
Sbjct: 243 ALVNKLAASGFETGPNL 259

[Close]

BLAST of Carg12101 vs. ExPASy TrEMBL
Match: A0A5D3D1R1 (21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.2e-83
Identity = 163/197 (82.74%), Postives = 177/197 (89.85%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           M+R P++R SI  LLA ISILPWLTHSA+TSYVQEAC VTLH DLCI+SLSPFSSTAKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVF+ AIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSR  FD+QMGDLTTWVSAALTDEDTCV+GFEGERG++V+L+RNRVVK  YITSNAL
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLA SGFETA N+
Sbjct: 181 ALVNKLATSGFETALNM 197

[Close]

BLAST of Carg12101 vs. ExPASy TrEMBL
Match: A0A1S3BSW5 (21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 3.2e-83
Identity = 163/197 (82.74%), Postives = 177/197 (89.85%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           M+R P++R SI  LLA ISILPWLTHSA+TSYVQEAC VTLH DLCI+SLSPFSSTAKRS
Sbjct: 1   MMRPPSTRASIAALLALISILPWLTHSAKTSYVQEACRVTLHQDLCIQSLSPFSSTAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVF+ AIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAALLGRLKNNKRMKGRNRAAVLDCVEVFDAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSR  FD+QMGDLTTWVSAALTDEDTCV+GFEGERG++V+L+RNRVVK  YITSNAL
Sbjct: 121 LRRLSRRNFDSQMGDLTTWVSAALTDEDTCVEGFEGERGKVVSLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLA SGFETA N+
Sbjct: 181 ALVNKLATSGFETALNM 197

[Close]

BLAST of Carg12101 vs. ExPASy TrEMBL
Match: A0A0A0KAX0 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 4.7e-82
Identity = 163/197 (82.74%), Postives = 173/197 (87.82%), Query Frame = 0


Query: 1   MLRAPTSRTSIVTLLAFISILPWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRS 60
           MLRAP+ R SI+ LLA ISILPWLTHSA+TSYVQEAC VT H DLCI+SLSPFSS AKRS
Sbjct: 1   MLRAPSRRASIIALLALISILPWLTHSAKTSYVQEACRVTRHQDLCIQSLSPFSSAAKRS 60

Query: 61  PTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGV 120
           PTKWARA VSVTITEAK VA  L RLKNN+RMKGRNRAAV DCVEVFE AIDELHRSLGV
Sbjct: 61  PTKWARAGVSVTITEAKKVAGLLGRLKNNKRMKGRNRAAVLDCVEVFEAAIDELHRSLGV 120

Query: 121 LRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGRLVNLIRNRVVKATYITSNAL 180
           LRRLSR  FDAQMGDLTTWVSAALTDEDTCV+GFEGE G++V L+RNRVVK  YITSNAL
Sbjct: 121 LRRLSRRNFDAQMGDLTTWVSAALTDEDTCVEGFEGEEGKVVTLLRNRVVKVGYITSNAL 180

Query: 181 ALVNKLAASGFETAPNL 198
           ALVNKLAAS FET  N+
Sbjct: 181 ALVNKLAASSFETTINM 197

[Close]

BLAST of Carg12101 vs. TAIR 10
Match: AT3G62820.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 168.7 bits (426), Expect = 4.7e-42
Identity = 96/194 (49.48%), Postives = 134/194 (69.07%), Query Frame = 0


Query: 2   LRAPTSRTSIVTLLAF-ISILPWLT-HSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKR 61
           ++ P S +    L+ F +S+ P  +  S + SYVQ ACSVT + DLC ++L PF+S AK 
Sbjct: 1   MKTPMSSSITFALVFFLLSLNPTSSLPSKRESYVQNACSVTRYQDLCAKTLLPFASVAKN 60

Query: 62  SPTKWARASVSVTITEAKAVARFLVRLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLG 121
           SP+KWARA VSV IT+ K V R L++ + +   K R+R A+SDC E+ +D++D LH+SL 
Sbjct: 61  SPSKWARAGVSVAITDNKDVLRHLLKTRLSTIGK-RDRIALSDCRELLQDSLDSLHKSLA 120

Query: 122 VLRRLSRSKFDAQMGDLTTWVSAALTDEDTCVDGFEGERGR---LVNLIRNRVVKATYIT 181
           VLR L  S+F  QM DL TW+S++LTD+DTC+DGFE    R    V +IR RV  + Y++
Sbjct: 121 VLRTLRASEFQQQMSDLATWLSSSLTDKDTCLDGFEKTSTRSSSTVRMIRKRVTTSMYLS 180

Query: 182 SNALALVNKLAASG 191
           SN+LAL+NKLAA+G
Sbjct: 181 SNSLALLNKLAANG 193

[Close]

BLAST of Carg12101 vs. TAIR 10
Match: AT2G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.0e-37
Identity = 82/164 (50.00%), Postives = 113/164 (68.90%), Query Frame = 0


Query: 30  TSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNN 89
           ++YV+ AC+VT +  LC+R+L PF+  A+ + +KWARASV+VTIT+ K V R L  LK  
Sbjct: 46  STYVRNACNVTRYNRLCVRTLWPFAIVARNNTSKWARASVAVTITDTKRVLRLL--LKTQ 105

Query: 90  RRMKGRN-RAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAALTDED 149
           R   G + R A+SDC E+F D++D L++SL VLR L+  +F  Q+ DL TW+SAALTD+D
Sbjct: 106 RSAVGESERIALSDCRELFVDSLDNLYKSLAVLRTLNADEFQRQISDLATWLSAALTDDD 165

Query: 150 TCVDGFE--GERGRLVNLIRNRVVKATYITSNALALVNKLAASG 191
           TC+DGFE    R R V ++R +  K   + SNALAL+ KLA  G
Sbjct: 166 TCLDGFEETSSRTRTVRMVRRKATKCMRLCSNALALLKKLAFDG 207

[Close]

BLAST of Carg12101 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 135.6 bits (340), Expect = 4.4e-32
Identity = 69/166 (41.57%), Postives = 109/166 (65.66%), Query Frame = 0


Query: 25  THSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLV 84
           T +  T +V+ +C+ T +P LC  SLS  +S  + SP   A A++++T+  AK  +  +V
Sbjct: 36  TTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPKLMAHAALNITLASAKVTSAMMV 95

Query: 85  RLKNNRRMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSKFDAQMGDLTTWVSAAL 144
           RL N+ R+K +  +A+ DCVE   D ++EL +S+G + +LS S ++  + D+ TWVSAAL
Sbjct: 96  RLSNS-RLKPKEVSAMRDCVEELGDTLEELRKSIGEMCQLSGSNYEVYISDIQTWVSAAL 155

Query: 145 TDEDTCVDGFEGE--RGRLVNLIRNRVVKATYITSNALALVNKLAA 189
           TD +TC DGFEGE   G++  L+R R++   ++TSNALAL+N  A+
Sbjct: 156 TDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNALALINHFAS 200

[Close]

BLAST of Carg12101 vs. TAIR 10
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 117.1 bits (292), Expect = 1.6e-26
Identity = 70/187 (37.43%), Postives = 106/187 (56.68%), Query Frame = 0


Query: 22  PWLTHSAQTSYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVAR 81
           P  T +    +++ +C+ TL+PD+C  SLS ++S  + SP + A+ ++ V++++AK+ A 
Sbjct: 34  PNTTTTNDLDFIRTSCNATLYPDVCFTSLSGYASAVQDSPARLAKLAIGVSLSQAKSTAA 93

Query: 82  FLVRLKNNRRMKGRN-----RAAVSDCVEVFEDAIDELHRSLGVLR-----------RLS 141
           FL +L  +      +      A + DCV   EDA+DE+  SL  LR           R S
Sbjct: 94  FLSKLSRSAAKYSGDGHQTASAVIRDCVSNVEDAVDEMRGSLRQLRDMNGRGGGTAARRS 153

Query: 142 RSKFDAQMGDLTTWVSAALTDEDTCVDGFE--GERGRLVNLIRNRVVKATYITSNALALV 191
              F  QM ++ TW+SAALTDEDTC DGFE   E G +   + +R+ +   +TSNALALV
Sbjct: 154 VETFRFQMSNVQTWMSAALTDEDTCTDGFEDMDEGGLIKTTVCDRLEEVKRLTSNALALV 213

[Close]

BLAST of Carg12101 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 117.1 bits (292), Expect = 1.6e-26
Identity = 61/163 (37.42%), Postives = 97/163 (59.51%), Query Frame = 0


Query: 31  SYVQEACSVTLHPDLCIRSLSPFSSTAKRSPTKWARASVSVTITEAKAVARFLVRLKNNR 90
           +++Q +C  T +P LC+ SLS +++  + SP + A  +++VT++ A++   F+ RL   +
Sbjct: 37  NFIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMK 96

Query: 91  RMKGRNRAAVSDCVEVFEDAIDELHRSLGVLRRLSRSK----FDAQMGDLTTWVSAALTD 150
            +K R   A+ DCVE   D +D L +S+  L+    +K    F   M +  TW SAALTD
Sbjct: 97  GLKKREVEAIKDCVEEMNDTVDRLTKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTD 156

Query: 151 EDTCVDGFEGE--RGRLVNLIRNRVVKATYITSNALALVNKLA 188
           E+TC DGF G    GR+ N +R R++   + TSNAL+L+N  A
Sbjct: 157 ENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINAFA 199

The following BLAST results are available for this feature:

BLAST of Carg12101 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023514751.1	1.9e-101	100.00	pectinesterase inhibitor 6 [Cucurbita pepo subsp. pepo] >KAG7030218.1 Pectineste...	[more]
KAG6593199.1	4.2e-101	100.00	Pectinesterase inhibitor 6, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023004663.1	4.2e-101	99.49	pectinesterase inhibitor 6 [Cucurbita maxima]	[more]
XP_022960074.1	1.6e-100	98.98	pectinesterase inhibitor 6-like [Cucurbita moschata]	[more]
XP_038896715.1	3.0e-83	84.69	pectinesterase inhibitor 6-like [Benincasa hispida]	[more]

BLAST of Carg12101 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22244	2.9e-36	50.00	Pectinesterase inhibitor 6 OS=Arabidopsis thaliana OX=3702 GN=PMEI6 PE=2 SV=1	[more]
P17407	4.1e-27	36.41	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI74	1.6e-26	37.06	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q84WE4	3.0e-25	35.75	Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1	[more]
Q9STY5	4.7e-23	35.29	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]

BLAST of Carg12101 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L076	2.0e-101	99.49	pectinesterase inhibitor 6 OS=Cucurbita maxima OX=3661 GN=LOC111497889 PE=4 SV=1	[more]
A0A6J1H9V5	7.7e-101	98.98	pectinesterase inhibitor 6-like OS=Cucurbita moschata OX=3662 GN=LOC111460929 PE...	[more]
A0A5D3D1R1	3.2e-83	82.74	21 kDa protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00630...	[more]
A0A1S3BSW5	3.2e-83	82.74	21 kDa protein OS=Cucumis melo OX=3656 GN=LOC103492810 PE=4 SV=1	[more]
A0A0A0KAX0	4.7e-82	82.74	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G397050 PE=4 S...	[more]

BLAST of Carg12101 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62820.1	4.7e-42	49.48	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G47670.1	2.0e-37	50.00	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62360.1	4.4e-32	41.57	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT2G01610.1	1.6e-26	37.43	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	1.6e-26	37.42	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 28..182 e-value: 8.4E-50 score: 181.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 32..182 e-value: 6.2E-35 score: 120.8
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 10..186 e-value: 4.1E-37 score: 125.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 27..188 e-value: 3.9E-38 score: 132.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 31..186
None	No IPR available	PANTHER	PTHR31080:SF158	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 18..181
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 18..181
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 36..188 e-value: 4.43244E-49 score: 154.904

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg12101-RA	Carg12101-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity