Carg11244 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg11244
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionprotein N-terminal asparagine amidohydrolase isoform X1
LocationCarg_Chr14: 5624448 .. 5627597 (-)
RNA-Seq ExpressionCarg11244
SyntenyCarg11244
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TACCATTATTTCCAGAAATCCTTTTACTCTTCTTCTCTCCCCCGTGATTCTTCATCTCTCTTCTCCTTCAACCTTCCATACCAGAGCCATGATTTTCGTCGACGGAGTACCCTTCACCGTCGAATCGTCCTCGTCAAATAAGGTACTTCCCTGCTTCAATCTTCACTGTTTTTCCCATTTCTGTGTTTAATTTTTTTATGAAATCGATTCAGAATTTGCCCCACTTTCTTGTTTAACGATGTGGCCATTTTCAATTCTATTAGCAGAGGAACATGAGGTTGGATGGATTTCGAGCTATTCAGTAATATAAATTTGTTGTTGACTGTTCTCTGATTGTATTTTCCACGATCAAGTCTGTGTACACTGTTTGACTTTCAGTGTTTAACTTTGTAATGATTCATCTGCTCATATTTGCTTGATCATTATCGTTGTTCTTGATCTTTGACCTAGTAAACGGTTTTTTTACTGATTAATTTTCTGAATATGAGTGGGTTTGAGAACATATTCAAGATCTATATAGCATGTTAGCATCTGATGGGCAAATTTTTTCTTTTAAATTAGATCACAAGCTTTACGCATCCGGCAGCCCATGCTTGGACAAAGTTGTATTTGTTTAACCATTCAGAAAGAACTGTGTTTCTGAGCAGGGAGCTGATGTCCTGTATGCTTTAATGGAGTGTCCTTTTCTTGTTGATGCCACAAAATCATTCAAGAGTATTCCAGAGATGAGGTTCACAGATTCTGAACAGTTTGGTGTGGAGAGATCAACAATGTGTAAATGGGTTTATCTGTTTCAGAAAGAATATGCCACTGTTGATCCTGCCTTGGTGGATGTATTTTTTCTTCCTTTCCCTCTCTTTGGTTAAGCTGCTCTTTCATGAATTTGAATTATGACCTTTGCTACTCAAACTTACTGGATAATTGATATGAATATCTGCGTAGGGACTTGAAGCACTGTGTTTTCAAAAGCTTCCTGTCGAACCGGCTTCTAGTAGTTGTAGCCAAACATCTAATGCTCCTGTGTCTCGACCACGACCTTACTCCGAACAATATCAATGTTATAGGTTGTACAACTATGTCGTTGAGTTTTAAAAAGTTATATAACGCTTTTGTGTCCCATTAGATGTCAGTAATACCCTGAGGTTAGGTTTTGAGATCACGAAAATCCTTCGAACGATGCCAAAAGATTATGATATTTATCCTTAGGACTACAACAAAGAACCTAAAGCAAGTTATTCCTTTACACATCCATAAGTATTTTCAATTGCATTCTGAATTAAAGTTGGTGGTCAGCTGGGTAAGAACTATTAGATCCTGATGTTGAAGTGTATGCAGCTTTCCTGATGTTGAAGTGTATGCAGCTTTCTGTTCTTTTCTAACTTTGTGTATTTATACCCACAATGCATAACTAAAGTTCGTTGGCACTGATGAAGCAACAACTTGTGTGGGCGTAGCTATTCGGAATCGAAAAAACGGAATGTAAGAATTATTTCTGAGAAGTTATATGTATTTTCTTCTGCTAATATCGCCATTGTGTTACAATAGTTGCCAACTTATGCTTCCTAATCTCATTTCCTGGCCTTCAGGACATCGGTTGCTCATATAGATTTTCCAAACATCATAGAAAACGCCCTCTCGCAGATGTTATCCTTAGTTGTTGATCCCTCTTCTGATGCCGAGTTAGATGTAATGTCCGTAATGTCGATCCTGCTTATCTAACAAATGAATTGATTTAAGCTAATGCTTGAGGACTGATTCGAATCTATTACACAATGTAGGTTCATTTAGTTGGAGGCTTTGAAGATGTTCTACTCAAAGTCTGGCCCAGAACCTATAACTTTGTGATTCATTTCCAAGGATGCCATCCACACAATCAGAATATTTGAATTGAATTGTATGCATTGGGGTTTGATGCAGGAAAGTAATGATATCACCGAGTTGGAAGATCGTAAAAAGACAGAGGGTCATTCATTTCCGCTGTGTAACAAGATTATCGAGCGTTTATGGACAAGACCAGAGACATTTCATCTTCAGACTCTTTGTGTTCTTCATCACAACACTCGGAGAGACTCTGAAGGGAATGCTTACCCCATCTTCAGTGGATTCTCGGTTAGTCTTTGATTCATCCAGTTTCATGTTCTGTTTATTTTCTTTCATCTTTTCGGGTTGAGCTATGATTTTCATCACAATTCACGAGCTGATTTATGAGCTATAAGATGATCAGCATTGTTTGAGTGCGTTCTGTAAAACAAATTAAACCTTTAGAATGCAAATTTAACTTAAATGTACTACTTTTCCTTTTGGTAATTCAGGTGAAAACTTCCGATGGATCTGTGTTCCCTGCTAGCTTTGACAGTACTTCAAGATGTCCAGATGAAGTTATTCGGAGAATTCGACTATCTTCCTCGTACGAGGATCCAAGTTTGAAGGGAAGACTGCTGGAGACATACGAAACTCATACCGATCAGTTCAAAATCGAACCATGCTGCTGGTAATCTGTCAACCTGTAACTTGCAAATCCTCCCCATGACCATTTTTACCTTAGGATTAACTCGGATGGACAACGAATGATGTTCTTGTGATATAATAGGTCGCCATGGAAACGATACATGGCTCTGTCTCTGCAACAGCTTTCCGATGCCGAAATCCTTCAATCATGTTCTACCTCTCCTTCTGCAGAAGCACCAGACTTTGTGGAAAATTCAAGAAGGTATATATACCATGTCGAATACCCTCAAAATAAGATGTATTTTACTTGTTTCTGCAATCATATGAATTTCAAAACGTTATTCCAATCATTCGTGTACCCGTTTCCCCCCATGAATGTTCATACGACTCGATGTATTAAAACCAAATGCTGCTCGTAACGTATGTGGAACTGAATATTGTCTCCCTCTCTTAACAGGCAGTGGGCTTATTTAGTGGAACACCCAGATTGGAGAGAAAGCTTCCCAGAGAAGAAGCCAAGAATTTTTAGAAGGGCTGCTGATGGGAAATGGGAAAGGAGCTTTAACTGACTTCTTCTCTGCTGTTATCTTTATGTGAATCCTTTTAGTTCTGTTATTTTATAACACTGTTCAACATATTTCCATTTAAAATCATGAGTGTGTAATTGTTATCTTCACCCAAATGAATTTGGGTCTTGCTTCC

mRNA sequence

TACCATTATTTCCAGAAATCCTTTTACTCTTCTTCTCTCCCCCGTGATTCTTCATCTCTCTTCTCCTTCAACCTTCCATACCAGAGCCATGATTTTCGTCGACGGAGTACCCTTCACCGTCGAATCGTCCTCGTCAAATAAGATCACAAGCTTTACGCATCCGGCAGCCCATGCTTGGACAAAGTTAAAGAACTGTGTTTCTGAGCAGGGAGCTGATGTCCTGTATGCTTTAATGGAGTGTCCTTTTCTTGTTGATGCCACAAAATCATTCAAGAGTATTCCAGAGATGAGGTTCACAGATTCTGAACAGTTTGGTGTGGAGAGATCAACAATGTGTAAATGGGTTTATCTGTTTCAGAAAGAATATGCCACTGTTGATCCTGCCTTGGTGGATTTCGTTGGCACTGATGAAGCAACAACTTGTGTGGGCGTAGCTATTCGGAATCGAAAAAACGGAATGACATCGGTTGCTCATATAGATTTTCCAAACATCATAGAAAACGCCCTCTCGCAGATGTTATCCTTAGTTGTTGATCCCTCTTCTGATGCCGAGTTAGATGTTCATTTAGTTGGAGGCTTTGAAGATGTTCTACTCAAAGTCTGGCCCAGAACCTATAACTTTGATGCCATCCACACAATCAGAATATTTGAATTGAATTGTATGCATTGGGGTTTGATGCAGGAAAGTAATGATATCACCGAGTTGGAAGATCGTAAAAAGACAGAGGGTCATTCATTTCCGCTGTGTAACAAGATTATCGAGCGTTTATGGACAAGACCAGAGACATTTCATCTTCAGACTCTTTGTGTTCTTCATCACAACACTCGGAGAGACTCTGAAGGGAATGCTTACCCCATCTTCAGTGGATTCTCGGTGAAAACTTCCGATGGATCTGTGTTCCCTGCTAGCTTTGACAGTACTTCAAGATGTCCAGATGAAGTTATTCGGAGAATTCGACTATCTTCCTCGTACGAGGATCCAAGTTTGAAGGGAAGACTGCTGGAGACATACGAAACTCATACCGATCAGTTCAAAATCGAACCATGCTGCTGGTCGCCATGGAAACGATACATGGCTCTGTCTCTGCAACAGCTTTCCGATGCCGAAATCCTTCAATCATGTTCTACCTCTCCTTCTGCAGAAGCACCAGACTTTGTGGAAAATTCAAGAAGGCAGTGGGCTTATTTAGTGGAACACCCAGATTGGAGAGAAAGCTTCCCAGAGAAGAAGCCAAGAATTTTTAGAAGGGCTGCTGATGGGAAATGGGAAAGGAGCTTTAACTGACTTCTTCTCTGCTGTTATCTTTATGTGAATCCTTTTAGTTCTGTTATTTTATAACACTGTTCAACATATTTCCATTTAAAATCATGAGTGTGTAATTGTTATCTTCACCCAAATGAATTTGGGTCTTGCTTCC

Coding sequence (CDS)

ATGATTTTCGTCGACGGAGTACCCTTCACCGTCGAATCGTCCTCGTCAAATAAGATCACAAGCTTTACGCATCCGGCAGCCCATGCTTGGACAAAGTTAAAGAACTGTGTTTCTGAGCAGGGAGCTGATGTCCTGTATGCTTTAATGGAGTGTCCTTTTCTTGTTGATGCCACAAAATCATTCAAGAGTATTCCAGAGATGAGGTTCACAGATTCTGAACAGTTTGGTGTGGAGAGATCAACAATGTGTAAATGGGTTTATCTGTTTCAGAAAGAATATGCCACTGTTGATCCTGCCTTGGTGGATTTCGTTGGCACTGATGAAGCAACAACTTGTGTGGGCGTAGCTATTCGGAATCGAAAAAACGGAATGACATCGGTTGCTCATATAGATTTTCCAAACATCATAGAAAACGCCCTCTCGCAGATGTTATCCTTAGTTGTTGATCCCTCTTCTGATGCCGAGTTAGATGTTCATTTAGTTGGAGGCTTTGAAGATGTTCTACTCAAAGTCTGGCCCAGAACCTATAACTTTGATGCCATCCACACAATCAGAATATTTGAATTGAATTGTATGCATTGGGGTTTGATGCAGGAAAGTAATGATATCACCGAGTTGGAAGATCGTAAAAAGACAGAGGGTCATTCATTTCCGCTGTGTAACAAGATTATCGAGCGTTTATGGACAAGACCAGAGACATTTCATCTTCAGACTCTTTGTGTTCTTCATCACAACACTCGGAGAGACTCTGAAGGGAATGCTTACCCCATCTTCAGTGGATTCTCGGTGAAAACTTCCGATGGATCTGTGTTCCCTGCTAGCTTTGACAGTACTTCAAGATGTCCAGATGAAGTTATTCGGAGAATTCGACTATCTTCCTCGTACGAGGATCCAAGTTTGAAGGGAAGACTGCTGGAGACATACGAAACTCATACCGATCAGTTCAAAATCGAACCATGCTGCTGGTCGCCATGGAAACGATACATGGCTCTGTCTCTGCAACAGCTTTCCGATGCCGAAATCCTTCAATCATGTTCTACCTCTCCTTCTGCAGAAGCACCAGACTTTGTGGAAAATTCAAGAAGGCAGTGGGCTTATTTAGTGGAACACCCAGATTGGAGAGAAAGCTTCCCAGAGAAGAAGCCAAGAATTTTTAGAAGGGCTGCTGATGGGAAATGGGAAAGGAGCTTTAACTGA

Protein sequence

MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKSFKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDAIHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN
Homology
BLAST of Carg11244 vs. NCBI nr
Match: KAG7018102.1 (Protein N-terminal asparagine amidohydrolase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 827.8 bits (2137), Expect = 4.0e-236
Identity = 398/398 (100.00%), Postives = 398/398 (100.00%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS
Sbjct: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR
Sbjct: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA
Sbjct: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
           IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC
Sbjct: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL
Sbjct: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS
Sbjct: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 399
           RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN
Sbjct: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 398

BLAST of Carg11244 vs. NCBI nr
Match: XP_022935589.1 (protein N-terminal asparagine amidohydrolase isoform X1 [Cucurbita moschata])

HSP 1 Score: 703.4 bits (1814), Expect = 1.1e-198
Identity = 354/398 (88.94%), Postives = 354/398 (88.94%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MIFVDGVPFTVESSSSN                KNCVSEQGADVLYALMECPFLVDATKS
Sbjct: 1   MIFVDGVPFTVESSSSN----------------KNCVSEQGADVLYALMECPFLVDATKS 60

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR
Sbjct: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLK          
Sbjct: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLK---------- 180

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
                             ESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC
Sbjct: 181 ------------------ESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL
Sbjct: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS
Sbjct: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 354

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 399
           RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN
Sbjct: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 354

BLAST of Carg11244 vs. NCBI nr
Match: XP_022982572.1 (protein N-terminal asparagine amidohydrolase isoform X1 [Cucurbita maxima])

HSP 1 Score: 689.9 bits (1779), Expect = 1.3e-194
Identity = 344/398 (86.43%), Postives = 353/398 (88.69%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MIFVDG+PFTVESSSSN                KNCVSEQGADVLYALMECPFLVDATKS
Sbjct: 49  MIFVDGIPFTVESSSSN----------------KNCVSEQGADVLYALMECPFLVDATKS 108

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FKSIPEMRFTDSEQFGVERST+CKWVY+FQKEYATVDPALVDFVGTDEATTCVGVA RNR
Sbjct: 109 FKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNR 168

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           +NGMTSVAHIDFPNIIENA+SQMLSLVVDPSSDAELDVHLVGGFEDVLLK          
Sbjct: 169 RNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDAELDVHLVGGFEDVLLK---------- 228

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
                             ESNDITELEDRKKTEG+SFPLCNKIIERLWTRPETFHLQTLC
Sbjct: 229 ------------------ESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETFHLQTLC 288

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           VLHHNTRRDS+GNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL
Sbjct: 289 VLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 348

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS
Sbjct: 349 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 402

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 399
           RRQWAYLVEHPDW+ESFP+KKPRIFRRAADGKWERSFN
Sbjct: 409 RRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 402

BLAST of Carg11244 vs. NCBI nr
Match: XP_023529133.1 (protein N-terminal asparagine amidohydrolase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 689.9 bits (1779), Expect = 1.3e-194
Identity = 349/398 (87.69%), Postives = 350/398 (87.94%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MIFVDGVPFTVESSSSN                KNCVSEQGADVLYALMECPFLVDATKS
Sbjct: 1   MIFVDGVPFTVESSSSN----------------KNCVSEQGADVLYALMECPFLVDATKS 60

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR
Sbjct: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLL           
Sbjct: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLL----------- 180

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
                            QESNDI ELEDRKKTEGHSFPLCNKIIE LWTRPETFHLQTLC
Sbjct: 181 -----------------QESNDIPELEDRKKTEGHSFPLCNKIIECLWTRPETFHLQTLC 240

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           VLHHNT RDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL
Sbjct: 241 VLHHNTWRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS
Sbjct: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 354

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 399
           RRQWAYLVEH DWRESFP+KKPRIFRRAADGKWERSFN
Sbjct: 361 RRQWAYLVEHSDWRESFPKKKPRIFRRAADGKWERSFN 354

BLAST of Carg11244 vs. NCBI nr
Match: XP_022935590.1 (protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita moschata] >KAG6581602.1 Protein N-terminal asparagine amidohydrolase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 688.0 bits (1774), Expect = 5.0e-194
Identity = 348/398 (87.44%), Postives = 348/398 (87.44%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MIFVDGVPFTVESSSSNK                      GADVLYALMECPFLVDATKS
Sbjct: 1   MIFVDGVPFTVESSSSNK----------------------GADVLYALMECPFLVDATKS 60

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR
Sbjct: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLK          
Sbjct: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLK---------- 180

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
                             ESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC
Sbjct: 181 ------------------ESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL
Sbjct: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS
Sbjct: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 348

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 399
           RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN
Sbjct: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 348

BLAST of Carg11244 vs. ExPASy Swiss-Prot
Match: O64876 (Protein N-terminal asparagine amidohydrolase OS=Arabidopsis thaliana OX=3702 GN=NTAN1 PE=2 SV=2)

HSP 1 Score: 359.0 bits (920), Expect = 6.9e-98
Identity = 190/395 (48.10%), Postives = 252/395 (63.80%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MI+V GV F  ESSS +  +                 S QG+ +L  +M  P +  A+ S
Sbjct: 1   MIYVGGVQFLDESSSFSLSS-----------------SSQGSSLLVDVMSHPVITLASDS 60

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FK++ E   +  E    E ST  ++VY+FQ+E+A V+PALVDFVGTDEATTCVG+ IRNR
Sbjct: 61  FKNLEEKNVSFDES-DSESSTKDRYVYIFQREFAVVNPALVDFVGTDEATTCVGLVIRNR 120

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           K+GMTSVAH+D P I++  +SQML LV+    DAELDVH+VGG+EDV +K      N D 
Sbjct: 121 KSGMTSVAHMDSPEIVDLGISQMLLLVLQDDVDAELDVHMVGGYEDVDIK------NADG 180

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
           +                          D  K EG+SFPLC K++E L  R E FH+QTL 
Sbjct: 181 V-------------------------GDYAKPEGYSFPLCCKLVETLQKRRENFHIQTLF 240

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           +L HNT+ DS+ N  PIF+G  V TS G++ PASF+ TSRCPDE++RRIR+SSS+ED S 
Sbjct: 241 ILGHNTKLDSQANTCPIFNGCLVNTSTGAILPASFNRTSRCPDEIVRRIRVSSSFEDSSW 300

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KG+LL+TY+T TD+F I PC W+         L QL+D EIL +CSTSPSAE PDFV + 
Sbjct: 301 KGKLLDTYDTKTDRFIIAPCRWTMRLIEYVWELNQLTDEEILTNCSTSPSAEGPDFVNSL 346

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWER 396
           RR W YL+++P+W ++FP ++PR+F R  DG W++
Sbjct: 361 RRNWGYLLKYPEWSKTFPRRQPRVFERTVDGHWKK 346

BLAST of Carg11244 vs. ExPASy Swiss-Prot
Match: Q64311 (Protein N-terminal asparagine amidohydrolase OS=Mus musculus OX=10090 GN=Ntan1 PE=1 SV=3)

HSP 1 Score: 117.5 bits (293), Expect = 3.5e-25
Identity = 92/316 (29.11%), Postives = 146/316 (46.20%), Query Frame = 0

Query: 86  VYLFQKEYATVDP--ALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQM 145
           +Y+ Q+E A   P    +  +G+D+ATTC  V +R+  NG T + H D  +  +  +  +
Sbjct: 47  LYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGSD-TKAEVPLI 106

Query: 146 LSLVVDPSSDAE---LDVHLVGGFEDVLLKVWPRTYNFDAIHTIRIFELNCMHWGLMQES 205
           +S +   S  AE   L+VHLVGGF D                                  
Sbjct: 107 MSSIKSFSEHAECGRLEVHLVGGFSD---------------------------------- 166

Query: 206 NDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSEGNAYPIFSG 265
                  DR+ ++     L ++++     + +  HL TLCV   N R ++E N +PI  G
Sbjct: 167 -------DRQLSQ----KLTHQLLSEFDKQDDDIHLVTLCVTELNDREENE-NHFPIIYG 226

Query: 266 FSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPC 325
            +V      ++ ASF    R P+E +R  R  +        G ++  Y+  T+Q +I PC
Sbjct: 227 IAVNIKTAEIYRASFQ--DRGPEEQLRAARALAG-------GPMISIYDAKTEQLRIGPC 286

Query: 326 CWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWRE-SFPE 385
            W+P+ + +   LQQ  D +IL+S STSP AE P FVE+ R    +L + P      FP 
Sbjct: 287 SWTPFPQ-VDFWLQQ-DDKQILESLSTSPLAEPPHFVEHIRSTLMFLKKFPSPENILFPG 304

Query: 386 KKPRIFRRAADGKWER 396
            K  ++++  DG WE+
Sbjct: 347 NKALLYKKNKDGLWEK 304

BLAST of Carg11244 vs. ExPASy Swiss-Prot
Match: Q28955 (Protein N-terminal asparagine amidohydrolase OS=Sus scrofa OX=9823 GN=NTAN1 PE=1 SV=4)

HSP 1 Score: 111.7 bits (278), Expect = 1.9e-23
Identity = 89/314 (28.34%), Postives = 142/314 (45.22%), Query Frame = 0

Query: 86  VYLFQKEYATVDP--ALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQM 145
           +Y+ Q+E A   P    V  +G+D+ATTC  V +R+  NG T + H D  +        M
Sbjct: 47  LYVQQRELAVTSPKDGSVCILGSDDATTCHIVVLRHTGNGATCLTHCDGTDTKAEVSLIM 106

Query: 146 LSL--VVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDAIHTIRIFELNCMHWGLMQESN 205
            S+    D +    L+VHLVGGF D                                   
Sbjct: 107 SSIKSFSDHTQRGRLEVHLVGGFSD----------------------------------- 166

Query: 206 DITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSEGNAYPIFSGF 265
                 DR+ ++     L ++++     + +  HL TLCV   N R ++E N +PI  G 
Sbjct: 167 ------DRQLSQ----KLTHQLLSEFDRQEDDIHLVTLCVTELNDREENE-NHFPIIYGI 226

Query: 266 SVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCC 325
           +V      ++ ASF    R P+E +R  R+ +        G ++  Y+  T+Q +I P  
Sbjct: 227 AVNVKTAEIYRASF--PDRGPEEELRAARVLTG-------GPMISIYDAKTEQLRIGPYS 286

Query: 326 WSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWRES-FPEK 385
           W P+  ++   LQQ  D +IL++ STSP AE P FVE+ R    +L ++P    + FP  
Sbjct: 287 WMPFP-HVDFWLQQ-DDKQILENLSTSPLAEPPHFVEHIRSTLMFLKKYPSPTNTLFPGN 303

Query: 386 KPRIFRRAADGKWE 395
           K  ++++  DG W+
Sbjct: 347 KALLYKKNEDGLWK 303

BLAST of Carg11244 vs. ExPASy Swiss-Prot
Match: Q96AB6 (Protein N-terminal asparagine amidohydrolase OS=Homo sapiens OX=9606 GN=NTAN1 PE=1 SV=3)

HSP 1 Score: 110.5 bits (275), Expect = 4.3e-23
Identity = 85/315 (26.98%), Postives = 140/315 (44.44%), Query Frame = 0

Query: 86  VYLFQKEYATVDP--ALVDFVGTDEATTCVGVAIRNRKNGMTSVAHIDFPNIIENA--LS 145
           +Y+ Q+E A   P    +  +G+D+ATTC  V +R+  NG T + H D  +       + 
Sbjct: 47  LYVQQRELAVTSPKDGSISILGSDDATTCHIVVLRHTGNGATCLTHCDGTDTKAEVPLIM 106

Query: 146 QMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDAIHTIRIFELNCMHWGLMQESN 205
             +    D +    L+VHLVGGF D                                   
Sbjct: 107 NSIKSFSDHAQCGRLEVHLVGGFSD----------------------------------- 166

Query: 206 DITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLCVLHHNTRRDSEGNAYPIFSGF 265
                 DR+ ++     L ++++     + +  HL TLCV   N R ++E N +P+  G 
Sbjct: 167 ------DRQLSQ----KLTHQLLSEFDRQEDDIHLVTLCVTELNDREENE-NHFPVIYGI 226

Query: 266 SVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSLKGRLLETYETHTDQFKIEPCC 325
           +V      ++ ASF    R P+E +R  R  +        G ++  Y+  T+Q +I P  
Sbjct: 227 AVNIKTAEIYRASFQ--DRGPEEQLRAARTLAG-------GPMISIYDAETEQLRIGPYS 286

Query: 326 WSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENSRRQWAYLVEHPDWRES-FPEK 385
           W+P+  ++   L Q  D +IL++ STSP AE P FVE+ R    +L +HP    + F   
Sbjct: 287 WTPFP-HVDFWLHQ-DDKQILENLSTSPLAEPPHFVEHIRSTLMFLKKHPSPAHTLFSGN 304

Query: 386 KPRIFRRAADGKWER 396
           K  ++++  DG WE+
Sbjct: 347 KALLYKKNEDGLWEK 304

BLAST of Carg11244 vs. ExPASy TrEMBL
Match: A0A6J1F5V2 (protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442418 PE=4 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 5.5e-199
Identity = 354/398 (88.94%), Postives = 354/398 (88.94%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MIFVDGVPFTVESSSSN                KNCVSEQGADVLYALMECPFLVDATKS
Sbjct: 1   MIFVDGVPFTVESSSSN----------------KNCVSEQGADVLYALMECPFLVDATKS 60

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR
Sbjct: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLK          
Sbjct: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLK---------- 180

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
                             ESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC
Sbjct: 181 ------------------ESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL
Sbjct: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS
Sbjct: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 354

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 399
           RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN
Sbjct: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 354

BLAST of Carg11244 vs. ExPASy TrEMBL
Match: A0A6J1IWZ0 (protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111481410 PE=4 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 6.3e-195
Identity = 344/398 (86.43%), Postives = 353/398 (88.69%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MIFVDG+PFTVESSSSN                KNCVSEQGADVLYALMECPFLVDATKS
Sbjct: 49  MIFVDGIPFTVESSSSN----------------KNCVSEQGADVLYALMECPFLVDATKS 108

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FKSIPEMRFTDSEQFGVERST+CKWVY+FQKEYATVDPALVDFVGTDEATTCVGVA RNR
Sbjct: 109 FKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNR 168

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           +NGMTSVAHIDFPNIIENA+SQMLSLVVDPSSDAELDVHLVGGFEDVLLK          
Sbjct: 169 RNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDAELDVHLVGGFEDVLLK---------- 228

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
                             ESNDITELEDRKKTEG+SFPLCNKIIERLWTRPETFHLQTLC
Sbjct: 229 ------------------ESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETFHLQTLC 288

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           VLHHNTRRDS+GNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL
Sbjct: 289 VLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 348

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS
Sbjct: 349 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 402

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 399
           RRQWAYLVEHPDW+ESFP+KKPRIFRRAADGKWERSFN
Sbjct: 409 RRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 402

BLAST of Carg11244 vs. ExPASy TrEMBL
Match: A0A6J1FB23 (protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111442418 PE=4 SV=1)

HSP 1 Score: 688.0 bits (1774), Expect = 2.4e-194
Identity = 348/398 (87.44%), Postives = 348/398 (87.44%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MIFVDGVPFTVESSSSNK                      GADVLYALMECPFLVDATKS
Sbjct: 1   MIFVDGVPFTVESSSSNK----------------------GADVLYALMECPFLVDATKS 60

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR
Sbjct: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLK          
Sbjct: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLK---------- 180

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
                             ESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC
Sbjct: 181 ------------------ESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL
Sbjct: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS
Sbjct: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 348

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 399
           RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN
Sbjct: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 348

BLAST of Carg11244 vs. ExPASy TrEMBL
Match: A0A6J1J4Y8 (protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111481410 PE=4 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 2.8e-190
Identity = 338/398 (84.92%), Postives = 347/398 (87.19%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MIFVDG+PFTVESSSSNK                      GADVLYALMECPFLVDATKS
Sbjct: 49  MIFVDGIPFTVESSSSNK----------------------GADVLYALMECPFLVDATKS 108

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FKSIPEMRFTDSEQFGVERST+CKWVY+FQKEYATVDPALVDFVGTDEATTCVGVA RNR
Sbjct: 109 FKSIPEMRFTDSEQFGVERSTICKWVYVFQKEYATVDPALVDFVGTDEATTCVGVAFRNR 168

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           +NGMTSVAHIDFPNIIENA+SQMLSLVVDPSSDAELDVHLVGGFEDVLLK          
Sbjct: 169 RNGMTSVAHIDFPNIIENAVSQMLSLVVDPSSDAELDVHLVGGFEDVLLK---------- 228

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
                             ESNDITELEDRKKTEG+SFPLCNKIIERLWTRPETFHLQTLC
Sbjct: 229 ------------------ESNDITELEDRKKTEGYSFPLCNKIIERLWTRPETFHLQTLC 288

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           VLHHNTRRDS+GNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL
Sbjct: 289 VLHHNTRRDSKGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 348

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS
Sbjct: 349 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 396

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 399
           RRQWAYLVEHPDW+ESFP+KKPRIFRRAADGKWERSFN
Sbjct: 409 RRQWAYLVEHPDWKESFPKKKPRIFRRAADGKWERSFN 396

BLAST of Carg11244 vs. ExPASy TrEMBL
Match: A0A6J1F542 (protein N-terminal asparagine amidohydrolase isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111442418 PE=4 SV=1)

HSP 1 Score: 651.4 bits (1679), Expect = 2.5e-183
Identity = 322/350 (92.00%), Postives = 322/350 (92.00%), Query Frame = 0

Query: 49  MECPFLVDATKSFKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDE 108
           MECPFLVDATKSFKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDE
Sbjct: 1   MECPFLVDATKSFKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDE 60

Query: 109 ATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVL 168
           ATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVL
Sbjct: 61  ATTCVGVAIRNRKNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVL 120

Query: 169 LKVWPRTYNFDAIHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLW 228
           LK                            ESNDITELEDRKKTEGHSFPLCNKIIERLW
Sbjct: 121 LK----------------------------ESNDITELEDRKKTEGHSFPLCNKIIERLW 180

Query: 229 TRPETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRR 288
           TRPETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRR
Sbjct: 181 TRPETFHLQTLCVLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRR 240

Query: 289 IRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTS 348
           IRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTS
Sbjct: 241 IRLSSSYEDPSLKGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTS 300

Query: 349 PSAEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 399
           PSAEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN
Sbjct: 301 PSAEAPDFVENSRRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWERSFN 322

BLAST of Carg11244 vs. TAIR 10
Match: AT2G44420.1 (protein N-terminal asparagine amidohydrolase family protein )

HSP 1 Score: 359.0 bits (920), Expect = 4.9e-99
Identity = 190/395 (48.10%), Postives = 252/395 (63.80%), Query Frame = 0

Query: 1   MIFVDGVPFTVESSSSNKITSFTHPAAHAWTKLKNCVSEQGADVLYALMECPFLVDATKS 60
           MI+V GV F  ESSS +  +                 S QG+ +L  +M  P +  A+ S
Sbjct: 1   MIYVGGVQFLDESSSFSLSS-----------------SSQGSSLLVDVMSHPVITLASDS 60

Query: 61  FKSIPEMRFTDSEQFGVERSTMCKWVYLFQKEYATVDPALVDFVGTDEATTCVGVAIRNR 120
           FK++ E   +  E    E ST  ++VY+FQ+E+A V+PALVDFVGTDEATTCVG+ IRNR
Sbjct: 61  FKNLEEKNVSFDES-DSESSTKDRYVYIFQREFAVVNPALVDFVGTDEATTCVGLVIRNR 120

Query: 121 KNGMTSVAHIDFPNIIENALSQMLSLVVDPSSDAELDVHLVGGFEDVLLKVWPRTYNFDA 180
           K+GMTSVAH+D P I++  +SQML LV+    DAELDVH+VGG+EDV +K      N D 
Sbjct: 121 KSGMTSVAHMDSPEIVDLGISQMLLLVLQDDVDAELDVHMVGGYEDVDIK------NADG 180

Query: 181 IHTIRIFELNCMHWGLMQESNDITELEDRKKTEGHSFPLCNKIIERLWTRPETFHLQTLC 240
           +                          D  K EG+SFPLC K++E L  R E FH+QTL 
Sbjct: 181 V-------------------------GDYAKPEGYSFPLCCKLVETLQKRRENFHIQTLF 240

Query: 241 VLHHNTRRDSEGNAYPIFSGFSVKTSDGSVFPASFDSTSRCPDEVIRRIRLSSSYEDPSL 300
           +L HNT+ DS+ N  PIF+G  V TS G++ PASF+ TSRCPDE++RRIR+SSS+ED S 
Sbjct: 241 ILGHNTKLDSQANTCPIFNGCLVNTSTGAILPASFNRTSRCPDEIVRRIRVSSSFEDSSW 300

Query: 301 KGRLLETYETHTDQFKIEPCCWSPWKRYMALSLQQLSDAEILQSCSTSPSAEAPDFVENS 360
           KG+LL+TY+T TD+F I PC W+         L QL+D EIL +CSTSPSAE PDFV + 
Sbjct: 301 KGKLLDTYDTKTDRFIIAPCRWTMRLIEYVWELNQLTDEEILTNCSTSPSAEGPDFVNSL 346

Query: 361 RRQWAYLVEHPDWRESFPEKKPRIFRRAADGKWER 396
           RR W YL+++P+W ++FP ++PR+F R  DG W++
Sbjct: 361 RRNWGYLLKYPEWSKTFPRRQPRVFERTVDGHWKK 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7018102.14.0e-236100.00Protein N-terminal asparagine amidohydrolase [Cucurbita argyrosperma subsp. argy... [more]
XP_022935589.11.1e-19888.94protein N-terminal asparagine amidohydrolase isoform X1 [Cucurbita moschata][more]
XP_022982572.11.3e-19486.43protein N-terminal asparagine amidohydrolase isoform X1 [Cucurbita maxima][more]
XP_023529133.11.3e-19487.69protein N-terminal asparagine amidohydrolase isoform X1 [Cucurbita pepo subsp. p... [more]
XP_022935590.15.0e-19487.44protein N-terminal asparagine amidohydrolase isoform X2 [Cucurbita moschata] >KA... [more]
Match NameE-valueIdentityDescription
O648766.9e-9848.10Protein N-terminal asparagine amidohydrolase OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q643113.5e-2529.11Protein N-terminal asparagine amidohydrolase OS=Mus musculus OX=10090 GN=Ntan1 P... [more]
Q289551.9e-2328.34Protein N-terminal asparagine amidohydrolase OS=Sus scrofa OX=9823 GN=NTAN1 PE=1... [more]
Q96AB64.3e-2326.98Protein N-terminal asparagine amidohydrolase OS=Homo sapiens OX=9606 GN=NTAN1 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1F5V25.5e-19988.94protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucurbita moschata OX... [more]
A0A6J1IWZ06.3e-19586.43protein N-terminal asparagine amidohydrolase isoform X1 OS=Cucurbita maxima OX=3... [more]
A0A6J1FB232.4e-19487.44protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita moschata OX... [more]
A0A6J1J4Y82.8e-19084.92protein N-terminal asparagine amidohydrolase isoform X2 OS=Cucurbita maxima OX=3... [more]
A0A6J1F5422.5e-18392.00protein N-terminal asparagine amidohydrolase isoform X3 OS=Cucurbita moschata OX... [more]
Match NameE-valueIdentityDescription
AT2G44420.14.9e-9948.10protein N-terminal asparagine amidohydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026750Protein N-terminal asparagine amidohydrolasePFAMPF14736N_Asn_amidohydcoord: 79..395
e-value: 1.2E-90
score: 303.4
IPR026750Protein N-terminal asparagine amidohydrolasePANTHERPTHR12498N-TERMINAL ASPARAGINE AMIDOHYDROLASEcoord: 1..395

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg11244-RACarg11244-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005634 nucleus
molecular_function GO:0008418 protein-N-terminal asparagine amidohydrolase activity