Carg10624 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg10624
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionSASA domain-containing protein
LocationCarg_Chr01: 12249780 .. 12251007 (+)
RNA-Seq ExpressionCarg10624
SyntenyCarg10624
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGTTCACCTTCCCCCCACTTCTCAACAACCACCTCCACCGACAGCCATTTTCCTCCTCGCCGGACAAAGCAACATGGCGGGGCGCGGCGGCGTCACCAACAGCACCCTAACGCACCGTCCCACGTGGGACCGCGTTGTTCCTCCCCAGTGCTCCCCCAATCCTTCTATCCTCCGCCTCGCCTCCGATCTCACCTGGGTTGAAGCTCGCGAGCCACTCCACGCCGACATTGATTTCCTCAAGACCAACGGGATTGGGCCTGGAATGCCATTCGCCAACACCATTCTCATGGATAAACCCGCCGGTCGGACGGTGATCGGTCTTGTCCCGTGCGCGATGGGCGGGACTTCGATTCAACAGTGGCAAAAGGGGTCCGATCTGTACACCCATTTATTGACCCGAGCCGAAGCGTCGATACTCAGCGGAGGCACGATTAAAGCGCTTCTGTGGTATCAGGGTGAAAGTGATACAGTGAATGCAGAAGATTCCGAGTTGTATGGTGGGAGATTGAAGAAGTTCTTCACTGACATTCGCTCCGATCTAAACATCCCATTCCTCCCAATTATTCAGGTTTTTATTTCCCTCTGTTTCTACTTCTTATTATAATTACTTTTACAGATTTAAAAAAGGAAAAGTTTTGATAAGTGACAAATGGTTAATGAGTCAGTAGCAAAAGTGGACAAGATAACAAGGCCAACTAACAATAGTAGAGAGAGTTTGTTAAAACGTCAACGTTTATGGTCGTAGGTGGGGATTGCATCAGGAGAGGGGCCGTATAAGGAAGGAGTGAGAAGAGGGCAATTAGGAATTGAGGTAATGAACGTGATGACTGTGGACGCATACGCATTGGGCTTGTCGTTTGAACCAGATGGGCTTCACTTAAATACCCCTTCTCAAGTTAAACTGGGTGGAATCTTGGCTGATGCATACCGCCGGTTTCCACCGCACCCACTGGCTGCTAGCCCATTAATAAGCGCTGCTTCCACGGCATCAGTTTTCAGTTTCTTTATTTCCATGTTTAGGACTATGACCTTCGTGTTGCTTCACCAAATTCTTTTGTCCTGACAGTGAAGTACACAACATTACATGCAATTATCATTGATGATGATTCAAAGCCTCCGGTGGCTGGCTTTTTAATTGGCCACCCATTCAAACAATATTCTTGAAAACGCCTGAGTTTTGTATCTTCCCTACCCTGTGTTCATGAATCTTACTTGGTTTTCAT

mRNA sequence

ATGGCCGTTCACCTTCCCCCCACTTCTCAACAACCACCTCCACCGACAGCCATTTTCCTCCTCGCCGGACAAAGCAACATGGCGGGGCGCGGCGGCGTCACCAACAGCACCCTAACGCACCGTCCCACGTGGGACCGCGTTGTTCCTCCCCAGTGCTCCCCCAATCCTTCTATCCTCCGCCTCGCCTCCGATCTCACCTGGGTTGAAGCTCGCGAGCCACTCCACGCCGACATTGATTTCCTCAAGACCAACGGGATTGGGCCTGGAATGCCATTCGCCAACACCATTCTCATGGATAAACCCGCCGGTCGGACGGTGATCGGTCTTGTCCCGTGCGCGATGGGCGGGACTTCGATTCAACAGTGGCAAAAGGGGTCCGATCTGTACACCCATTTATTGACCCGAGCCGAAGCGTCGATACTCAGCGGAGGCACGATTAAAGCGCTTCTGTGGTATCAGGGTGAAAGTGATACAGTGAATGCAGAAGATTCCGAGTTGTATGGTGGGAGATTGAAGAAGTTCTTCACTGACATTCGCTCCGATCTAAACATCCCATTCCTCCCAATTATTCAGGTGGGGATTGCATCAGGAGAGGGGCCGTATAAGGAAGGAGTGAGAAGAGGGCAATTAGGAATTGAGGTAATGAACGTGATGACTGTGGACGCATACGCATTGGGCTTGTCGTTTGAACCAGATGGGCTTCACTTAAATACCCCTTCTCAAGTTAAACTGGGTGGAATCTTGGCTGATGCATACCGCCGGTTTCCACCGCACCCACTGGCTGCTAGCCCATTAATAAGCGCTGCTTCCACGGCATCAGTTTTCAGTTTCTTTATTTCCATGTTTAGGACTATGACCTTCGTGTTGCTTCACCAAATTCTTTTGTCCTGACAGTGAAGTACACAACATTACATGCAATTATCATTGATGATGATTCAAAGCCTCCGGTGGCTGGCTTTTTAATTGGCCACCCATTCAAACAATATTCTTGAAAACGCCTGAGTTTTGTATCTTCCCTACCCTGTGTTCATGAATCTTACTTGGTTTTCAT

Coding sequence (CDS)

ATGGCCGTTCACCTTCCCCCCACTTCTCAACAACCACCTCCACCGACAGCCATTTTCCTCCTCGCCGGACAAAGCAACATGGCGGGGCGCGGCGGCGTCACCAACAGCACCCTAACGCACCGTCCCACGTGGGACCGCGTTGTTCCTCCCCAGTGCTCCCCCAATCCTTCTATCCTCCGCCTCGCCTCCGATCTCACCTGGGTTGAAGCTCGCGAGCCACTCCACGCCGACATTGATTTCCTCAAGACCAACGGGATTGGGCCTGGAATGCCATTCGCCAACACCATTCTCATGGATAAACCCGCCGGTCGGACGGTGATCGGTCTTGTCCCGTGCGCGATGGGCGGGACTTCGATTCAACAGTGGCAAAAGGGGTCCGATCTGTACACCCATTTATTGACCCGAGCCGAAGCGTCGATACTCAGCGGAGGCACGATTAAAGCGCTTCTGTGGTATCAGGGTGAAAGTGATACAGTGAATGCAGAAGATTCCGAGTTGTATGGTGGGAGATTGAAGAAGTTCTTCACTGACATTCGCTCCGATCTAAACATCCCATTCCTCCCAATTATTCAGGTGGGGATTGCATCAGGAGAGGGGCCGTATAAGGAAGGAGTGAGAAGAGGGCAATTAGGAATTGAGGTAATGAACGTGATGACTGTGGACGCATACGCATTGGGCTTGTCGTTTGAACCAGATGGGCTTCACTTAAATACCCCTTCTCAAGTTAAACTGGGTGGAATCTTGGCTGATGCATACCGCCGGTTTCCACCGCACCCACTGGCTGCTAGCCCATTAATAAGCGCTGCTTCCACGGCATCAGTTTTCAGTTTCTTTATTTCCATGTTTAGGACTATGACCTTCGTGTTGCTTCACCAAATTCTTTTGTCCTGA

Protein sequence

MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILRLASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQQWQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRSDLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPSQVKLGGILADAYRRFPPHPLAASPLISAASTASVFSFFISMFRTMTFVLLHQILLS
Homology
BLAST of Carg10624 vs. NCBI nr
Match: KAG7037782.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 599.7 bits (1545), Expect = 1.3e-167
Identity = 296/296 (100.00%), Postives = 296/296 (100.00%), Query Frame = 0

Query: 1   MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR 60
           MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR
Sbjct: 1   MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR 60

Query: 61  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 120
           LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ
Sbjct: 61  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 120

Query: 121 QWQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 180
           QWQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS
Sbjct: 121 QWQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 180

Query: 181 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 240
           DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS
Sbjct: 181 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 240

Query: 241 QVKLGGILADAYRRFPPHPLAASPLISAASTASVFSFFISMFRTMTFVLLHQILLS 297
           QVKLGGILADAYRRFPPHPLAASPLISAASTASVFSFFISMFRTMTFVLLHQILLS
Sbjct: 241 QVKLGGILADAYRRFPPHPLAASPLISAASTASVFSFFISMFRTMTFVLLHQILLS 296

BLAST of Carg10624 vs. NCBI nr
Match: KAG6608446.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 591.3 bits (1523), Expect = 4.7e-165
Identity = 293/297 (98.65%), Postives = 296/297 (99.66%), Query Frame = 0

Query: 1   MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR 60
           MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR
Sbjct: 10  MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR 69

Query: 61  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 120
           LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ
Sbjct: 70  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 129

Query: 121 QWQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 180
           QWQKGS+LYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS
Sbjct: 130 QWQKGSNLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 189

Query: 181 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 240
           DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS
Sbjct: 190 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 249

Query: 241 QVKLGGILADAYRRFPPHPLAASPLI-SAASTASVFSFFISMFRTMTFVLLHQILLS 297
           QVKLGGILADAYRRFPPHPLAASPL+ SAASTASVFSFFISMFRTMTF+LLHQILLS
Sbjct: 250 QVKLGGILADAYRRFPPHPLAASPLLRSAASTASVFSFFISMFRTMTFLLLHQILLS 306

BLAST of Carg10624 vs. NCBI nr
Match: XP_022941448.1 (probable carbohydrate esterase At4g34215 [Cucurbita moschata])

HSP 1 Score: 580.9 bits (1496), Expect = 6.4e-162
Identity = 287/296 (96.96%), Postives = 290/296 (97.97%), Query Frame = 0

Query: 1   MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR 60
           MA HLP TSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWD VVPPQCSPNPSILR
Sbjct: 38  MAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDGVVPPQCSPNPSILR 97

Query: 61  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 120
           LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ
Sbjct: 98  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 157

Query: 121 QWQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 180
           QWQKGS+LYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRL KFFTDIRS
Sbjct: 158 QWQKGSNLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLNKFFTDIRS 217

Query: 181 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 240
           DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS
Sbjct: 218 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 277

Query: 241 QVKLGGILADAYRRFPPHPLAASPLISAASTASVFSFFISMFRTMTFVLLHQILLS 297
           QVKLGG+LADAYRRFPPHPLAASPL SAASTASV SFFISMFRT+TFVLLHQILLS
Sbjct: 278 QVKLGGVLADAYRRFPPHPLAASPLRSAASTASVHSFFISMFRTLTFVLLHQILLS 333

BLAST of Carg10624 vs. NCBI nr
Match: XP_023525343.1 (probable carbohydrate esterase At4g34215 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 572.4 bits (1474), Expect = 2.3e-159
Identity = 284/297 (95.62%), Postives = 290/297 (97.64%), Query Frame = 0

Query: 1   MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR 60
           MA HLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWD VVPPQCSPNPSILR
Sbjct: 10  MAAHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDGVVPPQCSPNPSILR 69

Query: 61  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 120
           LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGG+SIQ
Sbjct: 70  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGSSIQ 129

Query: 121 QWQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 180
           QWQKGS+LYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFF+DIRS
Sbjct: 130 QWQKGSNLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFSDIRS 189

Query: 181 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 240
           DL+IPFLPIIQVGIASGEGPYKEGVRRGQ GIEVMNVMTVDAYALGLSFEPDGLHLNT S
Sbjct: 190 DLHIPFLPIIQVGIASGEGPYKEGVRRGQFGIEVMNVMTVDAYALGLSFEPDGLHLNTHS 249

Query: 241 QVKLGGILADAYRRFPPHPLAASPLI-SAASTASVFSFFISMFRTMTFVLLHQILLS 297
           QVKLGG+LADAYRRFPPHPLAASPL+ SAASTASVFSFFISMF TMTFVLL QILLS
Sbjct: 250 QVKLGGVLADAYRRFPPHPLAASPLLRSAASTASVFSFFISMFSTMTFVLLQQILLS 306

BLAST of Carg10624 vs. NCBI nr
Match: XP_022982273.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 569.7 bits (1467), Expect = 1.5e-158
Identity = 280/296 (94.59%), Postives = 286/296 (96.62%), Query Frame = 0

Query: 1   MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR 60
           MA HLP TSQQPPPPTAIFLLAGQSNMAGRGGV+N+TLTHRPTWD VVPPQCSPNPSILR
Sbjct: 10  MAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVSNTTLTHRPTWDGVVPPQCSPNPSILR 69

Query: 61  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 120
           LASDLTWVEAREPLHADIDFLK NGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ
Sbjct: 70  LASDLTWVEAREPLHADIDFLKINGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 129

Query: 121 QWQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 180
           QWQKGS+LY HLLTRAEASIL GGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS
Sbjct: 130 QWQKGSNLYNHLLTRAEASILGGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 189

Query: 181 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 240
           DLNIPFLPIIQVGIASGEGPYKEGVRRGQ GIEVMNVMTVDAYALGLSFEPDGLHLNTPS
Sbjct: 190 DLNIPFLPIIQVGIASGEGPYKEGVRRGQFGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 249

Query: 241 QVKLGGILADAYRRFPPHPLAASPLISAASTASVFSFFISMFRTMTFVLLHQILLS 297
           QVKLGG+LADAYRRFPPHPLAASPL +AASTASV+SF ISMFRTMTFV LHQILLS
Sbjct: 250 QVKLGGVLADAYRRFPPHPLAASPLRNAASTASVYSFCISMFRTMTFVFLHQILLS 305

BLAST of Carg10624 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 5.4e-71
Identity = 132/246 (53.66%), Postives = 168/246 (68.29%), Query Frame = 0

Query: 7   PTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILRLASDLT 66
           P  Q P PP  IF+L+GQSNMAGRGGV      +R  WD+++PP+C+PN SILRL++DL 
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 67  WVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQQWQKGS 126
           W EA EPLH DID  K  G+GPGM FAN +         VIGLVPCA GGT+I++W++GS
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGS 132

Query: 127 DLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRSDLNIPF 186
            LY  ++ R E S   GG IKA+LWYQGESD ++  D+E YG  + +   ++R DLN+P 
Sbjct: 133 HLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPS 192

Query: 187 LPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPSQVKLGG 246
           LPIIQV IASG G Y + VR  QLG+++ NV+ VD  A GL  + D LHL T +QV+LG 
Sbjct: 193 LPIIQVAIASG-GGYIDKVREAQLGLKLSNVVCVD--AKGLPLKSDNLHLTTEAQVQLGL 252

Query: 247 ILADAY 253
            LA AY
Sbjct: 253 SLAQAY 255

BLAST of Carg10624 vs. ExPASy TrEMBL
Match: A0A6J1FS50 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111446742 PE=4 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 3.1e-162
Identity = 287/296 (96.96%), Postives = 290/296 (97.97%), Query Frame = 0

Query: 1   MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR 60
           MA HLP TSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWD VVPPQCSPNPSILR
Sbjct: 38  MAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDGVVPPQCSPNPSILR 97

Query: 61  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 120
           LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ
Sbjct: 98  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 157

Query: 121 QWQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 180
           QWQKGS+LYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRL KFFTDIRS
Sbjct: 158 QWQKGSNLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLNKFFTDIRS 217

Query: 181 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 240
           DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS
Sbjct: 218 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 277

Query: 241 QVKLGGILADAYRRFPPHPLAASPLISAASTASVFSFFISMFRTMTFVLLHQILLS 297
           QVKLGG+LADAYRRFPPHPLAASPL SAASTASV SFFISMFRT+TFVLLHQILLS
Sbjct: 278 QVKLGGVLADAYRRFPPHPLAASPLRSAASTASVHSFFISMFRTLTFVLLHQILLS 333

BLAST of Carg10624 vs. ExPASy TrEMBL
Match: A0A6J1J4F1 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111481152 PE=4 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 7.1e-159
Identity = 280/296 (94.59%), Postives = 286/296 (96.62%), Query Frame = 0

Query: 1   MAVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILR 60
           MA HLP TSQQPPPPTAIFLLAGQSNMAGRGGV+N+TLTHRPTWD VVPPQCSPNPSILR
Sbjct: 10  MAAHLPLTSQQPPPPTAIFLLAGQSNMAGRGGVSNTTLTHRPTWDGVVPPQCSPNPSILR 69

Query: 61  LASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 120
           LASDLTWVEAREPLHADIDFLK NGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ
Sbjct: 70  LASDLTWVEAREPLHADIDFLKINGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQ 129

Query: 121 QWQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 180
           QWQKGS+LY HLLTRAEASIL GGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS
Sbjct: 130 QWQKGSNLYNHLLTRAEASILGGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRS 189

Query: 181 DLNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 240
           DLNIPFLPIIQVGIASGEGPYKEGVRRGQ GIEVMNVMTVDAYALGLSFEPDGLHLNTPS
Sbjct: 190 DLNIPFLPIIQVGIASGEGPYKEGVRRGQFGIEVMNVMTVDAYALGLSFEPDGLHLNTPS 249

Query: 241 QVKLGGILADAYRRFPPHPLAASPLISAASTASVFSFFISMFRTMTFVLLHQILLS 297
           QVKLGG+LADAYRRFPPHPLAASPL +AASTASV+SF ISMFRTMTFV LHQILLS
Sbjct: 250 QVKLGGVLADAYRRFPPHPLAASPLRNAASTASVYSFCISMFRTMTFVFLHQILLS 305

BLAST of Carg10624 vs. ExPASy TrEMBL
Match: A0A0A0L4Z7 (SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G641680 PE=4 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.8e-125
Identity = 226/283 (79.86%), Postives = 245/283 (86.57%), Query Frame = 0

Query: 5   LPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILRLASD 64
           +P TSQQP PPT IFLLAGQSNMAGRGGVTNSTLTH PTWD VVPPQCSP P ILRLA+D
Sbjct: 15  IPSTSQQPSPPTDIFLLAGQSNMAGRGGVTNSTLTHHPTWDGVVPPQCSPTPYILRLAAD 74

Query: 65  LTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQQWQK 124
           LTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKP GRTVIGLVPCAMGGTSI++WQK
Sbjct: 75  LTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPGGRTVIGLVPCAMGGTSIKEWQK 134

Query: 125 GSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRSDLNI 184
           GS+LY HLL+RA+AS+LSGG IKALLWYQGESDT NAEDSELYGGRLKKFFT IRSDL I
Sbjct: 135 GSNLYNHLLSRADASVLSGGKIKALLWYQGESDTENAEDSELYGGRLKKFFTGIRSDLKI 194

Query: 185 PFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPSQVKL 244
           P LPIIQVGIASGEG YKEGVRRGQ GI+++NVM VD  ALGL  EPDGLHL T SQV+L
Sbjct: 195 PLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVD--ALGLPLEPDGLHLTTTSQVRL 254

Query: 245 GGILADAYRRFPPHPLAASPLISAASTASVFSFFISMFRTMTF 288
           GG+LADAYRRFP HPL A+PL +AA  + + + F      + F
Sbjct: 255 GGLLADAYRRFPSHPL-ATPLTNAAPISRISTIFFPFVGFLRF 294

BLAST of Carg10624 vs. ExPASy TrEMBL
Match: A0A5A7VA07 (Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003010 PE=4 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 8.8e-125
Identity = 226/288 (78.47%), Postives = 248/288 (86.11%), Query Frame = 0

Query: 2   AVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILRL 61
           A  +P  SQQP PPT IFLLAGQSNMAGRGGVTN TLTH+PTWD VVPPQCSP P ILR 
Sbjct: 13  AAQIPSASQQPSPPTDIFLLAGQSNMAGRGGVTNGTLTHQPTWDGVVPPQCSPTPYILRF 72

Query: 62  ASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQQ 121
           A+DLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKP GRTVIGLVPCA+GGTSI++
Sbjct: 73  AADLTWVEAREPLHADIDFLKTNGIGPGMPFANAILMDKPGGRTVIGLVPCAIGGTSIKE 132

Query: 122 WQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRSD 181
           WQKGS+LY HLL+RAEASIL GG IKALLWYQGESDT NAEDSELYGGRLKKF TDIRSD
Sbjct: 133 WQKGSNLYNHLLSRAEASILRGGKIKALLWYQGESDTENAEDSELYGGRLKKFLTDIRSD 192

Query: 182 LNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPSQ 241
           L IP LPIIQVGIASGEG YKEGVRRGQ GI+++NVM VD  ALGL  EPDGLHL TPSQ
Sbjct: 193 LKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVD--ALGLPLEPDGLHLTTPSQ 252

Query: 242 VKLGGILADAYRRFPPHPLAASPLISAASTASVFS-FFISMFRTMTFV 289
           V+LGG+LA AYRRFP HPL A+PL +AA  +++ S F +S++   TF+
Sbjct: 253 VRLGGLLAHAYRRFPSHPL-ATPLTNAAPISTIISTFLLSIWWIFTFL 297

BLAST of Carg10624 vs. ExPASy TrEMBL
Match: A0A1S3BVE3 (probable carbohydrate esterase At4g34215 OS=Cucumis melo OX=3656 GN=LOC103493577 PE=4 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 8.8e-125
Identity = 226/288 (78.47%), Postives = 248/288 (86.11%), Query Frame = 0

Query: 2   AVHLPPTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILRL 61
           A  +P  SQQP PPT IFLLAGQSNMAGRGGVTN TLTH+PTWD VVPPQCSP P ILR 
Sbjct: 13  AAQIPSASQQPSPPTDIFLLAGQSNMAGRGGVTNGTLTHQPTWDGVVPPQCSPTPYILRF 72

Query: 62  ASDLTWVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQQ 121
           A+DLTWVEAREPLHADIDFLKTNGIGPGMPFAN ILMDKP GRTVIGLVPCA+GGTSI++
Sbjct: 73  AADLTWVEAREPLHADIDFLKTNGIGPGMPFANAILMDKPGGRTVIGLVPCAIGGTSIKE 132

Query: 122 WQKGSDLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRSD 181
           WQKGS+LY HLL+RAEASIL GG IKALLWYQGESDT NAEDSELYGGRLKKF TDIRSD
Sbjct: 133 WQKGSNLYNHLLSRAEASILRGGKIKALLWYQGESDTENAEDSELYGGRLKKFLTDIRSD 192

Query: 182 LNIPFLPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPSQ 241
           L IP LPIIQVGIASGEG YKEGVRRGQ GI+++NVM VD  ALGL  EPDGLHL TPSQ
Sbjct: 193 LKIPLLPIIQVGIASGEGEYKEGVRRGQFGIDLVNVMIVD--ALGLPLEPDGLHLTTPSQ 252

Query: 242 VKLGGILADAYRRFPPHPLAASPLISAASTASVFS-FFISMFRTMTFV 289
           V+LGG+LA AYRRFP HPL A+PL +AA  +++ S F +S++   TF+
Sbjct: 253 VRLGGLLAHAYRRFPSHPL-ATPLTNAAPISTIISTFLLSIWWIFTFL 297

BLAST of Carg10624 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 281.2 bits (718), Expect = 9.7e-76
Identity = 137/242 (56.61%), Postives = 176/242 (72.73%), Query Frame = 0

Query: 17  AIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILRLASDLTWVEAREPLHA 76
           +IF+LAGQSNMAGRGGV N T T+   WD V+PP+C  NPSILRL S L W EA+EPLH 
Sbjct: 30  SIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSKLEWKEAKEPLHV 89

Query: 77  DIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQQWQKGSDLYTHLLTRA 136
           DID  KTNG+GPGMPFAN ++     G+  +GLVPC++GGT + QWQKG  LY   + RA
Sbjct: 90  DIDINKTNGVGPGMPFANRVV--NRFGQ--VGLVPCSIGGTKLSQWQKGEFLYEETVKRA 149

Query: 137 EASILS--GGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRSDLNIPFLPIIQVGI 196
           +A++ S  GG+ +A+LWYQGESDTV+  D+ +Y  RL KFF+D+R+DL  P LPIIQV +
Sbjct: 150 KAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFFSDLRNDLQHPNLPIIQVAL 209

Query: 197 ASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPSQVKLGGILADAYRR 256
           A+G GPY + VR+ QL  ++ NV  VD  A GL  EPDGLHL T SQV+LG ++A+++  
Sbjct: 210 ATGAGPYLDAVRKAQLKTDLENVYCVD--ARGLPLEPDGLHLTTSSQVQLGHMIAESFLA 265

BLAST of Carg10624 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 269.2 bits (687), Expect = 3.8e-72
Identity = 132/246 (53.66%), Postives = 168/246 (68.29%), Query Frame = 0

Query: 7   PTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILRLASDLT 66
           P  Q P PP  IF+L+GQSNMAGRGGV      +R  WD+++PP+C+PN SILRL++DL 
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 67  WVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQQWQKGS 126
           W EA EPLH DID  K  G+GPGM FAN +         VIGLVPCA GGT+I++W++GS
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGS 132

Query: 127 DLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRSDLNIPF 186
            LY  ++ R E S   GG IKA+LWYQGESD ++  D+E YG  + +   ++R DLN+P 
Sbjct: 133 HLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPS 192

Query: 187 LPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPSQVKLGG 246
           LPIIQV IASG G Y + VR  QLG+++ NV+ VD  A GL  + D LHL T +QV+LG 
Sbjct: 193 LPIIQVAIASG-GGYIDKVREAQLGLKLSNVVCVD--AKGLPLKSDNLHLTTEAQVQLGL 252

Query: 247 ILADAY 253
            LA AY
Sbjct: 253 SLAQAY 255

BLAST of Carg10624 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 269.2 bits (687), Expect = 3.8e-72
Identity = 132/246 (53.66%), Postives = 168/246 (68.29%), Query Frame = 0

Query: 7   PTSQQPPPPTAIFLLAGQSNMAGRGGVTNSTLTHRPTWDRVVPPQCSPNPSILRLASDLT 66
           P  Q P PP  IF+L+GQSNMAGRGGV      +R  WD+++PP+C+PN SILRL++DL 
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 67  WVEAREPLHADIDFLKTNGIGPGMPFANTILMDKPAGRTVIGLVPCAMGGTSIQQWQKGS 126
           W EA EPLH DID  K  G+GPGM FAN +         VIGLVPCA GGT+I++W++GS
Sbjct: 73  WEEAHEPLHVDIDTGKVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWERGS 132

Query: 127 DLYTHLLTRAEASILSGGTIKALLWYQGESDTVNAEDSELYGGRLKKFFTDIRSDLNIPF 186
            LY  ++ R E S   GG IKA+LWYQGESD ++  D+E YG  + +   ++R DLN+P 
Sbjct: 133 HLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLRHDLNLPS 192

Query: 187 LPIIQVGIASGEGPYKEGVRRGQLGIEVMNVMTVDAYALGLSFEPDGLHLNTPSQVKLGG 246
           LPIIQV IASG G Y + VR  QLG+++ NV+ VD  A GL  + D LHL T +QV+LG 
Sbjct: 193 LPIIQVAIASG-GGYIDKVREAQLGLKLSNVVCVD--AKGLPLKSDNLHLTTEAQVQLGL 252

Query: 247 ILADAY 253
            LA AY
Sbjct: 253 SLAQAY 255

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037782.11.3e-167100.00putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
KAG6608446.14.7e-16598.65putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022941448.16.4e-16296.96probable carbohydrate esterase At4g34215 [Cucurbita moschata][more]
XP_023525343.12.3e-15995.62probable carbohydrate esterase At4g34215 [Cucurbita pepo subsp. pepo][more]
XP_022982273.11.5e-15894.59probable carbohydrate esterase At4g34215 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8L9J95.4e-7153.66Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g... [more]
Match NameE-valueIdentityDescription
A0A6J1FS503.1e-16296.96probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1J4F17.1e-15994.59probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC11148... [more]
A0A0A0L4Z71.8e-12579.86SASA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G641680 PE=4 S... [more]
A0A5A7VA078.8e-12578.47Putative carbohydrate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BVE38.8e-12578.47probable carbohydrate esterase At4g34215 OS=Cucumis melo OX=3656 GN=LOC103493577... [more]
Match NameE-valueIdentityDescription
AT3G53010.19.7e-7656.61Domain of unknown function (DUF303) [more]
AT4G34215.13.8e-7253.66Domain of unknown function (DUF303) [more]
AT4G34215.23.8e-7253.66Domain of unknown function (DUF303) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 14..254
e-value: 1.4E-61
score: 210.4
IPR005181Sialate O-acetylesterase domainPFAMPF03629SASAcoord: 16..252
e-value: 4.3E-78
score: 262.0
NoneNo IPR availablePANTHERPTHR31988ESTERASE, PUTATIVE (DUF303)-RELATEDcoord: 6..254
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 18..255

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg10624-RACarg10624-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane