Carg09654 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg09654
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	NAD(P)-binding Rossmann-fold superfamily protein
Location	Carg_Chr14: 3670009 .. 3673027 (-)
RNA-Seq Expression	Carg09654
Synteny	Carg09654

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAAGAGATAATCTGGAGTATGTTTTCGTCCATATATTAAGGTAATAACTTTTTAACGCAAGAATCATGAGAATATTTTAACGCAACCAATGATGCACACAACGACACTCCTGTCAATCTTGTGACCAGAGTTCTGTGCCAATTTTCTCTTTAATCCTTCATTGTTCTTATCAGGGTTTCATCGGTTTTCGGATCCGGTACGCTCTTCAATTATTTTCGGTCTTCTTCTCTTCATTTTTCATCGATTAACCGGTTCTTCGATGATCCATATGCAGAGTTCAATTTCTACCCCCGAATCGAAGCTCCATTTGCTTTGTACACATGGAGAGAGCTACCAAGAACGTGCTGCTTTCTTCTAATGGGGATGAGATATCCAAGAATCTGGCTCTACATCTTGCCAAAAGGGGTTGCAGGTTTAGCATTGAGCTTTGATCTTACTGCCTTTTTTTGGATGAAATTTTTGGTTATTGTGTTCTTTCTTTCTTCTGTTTCATTGTTGTGTTTAGATTGGTTTTGTTGGGAAATGAGACTGTTCTTCGCAGAACAAGTAAGGAGATAGTTGAATCCGTAAAAGGTGTCCTGCCGATTCAGGTTGTTGGCTTGGACATGGAAGTAGAGGGAGAGGCGGATTTTAGTGAGGCTGTGAACAGGGCTTGCAGTATTTTGGGCGCTTTGGATGCTTTTGTTCATGCTTATTCTTATGATGGTACAGCTTATCTCTAACTCTTTCACCCATTTTCTCTGTTCTCCATGCTTACTCTTACTTGTTTTAGTTTTTCTATTGCTTAGTTCATTGTTAATTCGTCTCTAAACAGAGGTTTTAGGCTCTTGTTTGGACATGATTCGATATTTGAATAAAGATTTAGCCTCTTGTGAAGACTGAATAGAGATCGTAACAAGCCAAGCCCATTGCTAGTAGATATTGTCTGCTTTAGTCCATTACGTATCACCGTTAGCCTCATGGTTTTAGAACACGTCTACTAGGGAGAGGTTTCCATACCTTTTTGTAAGGAATGTTTCATTCCCCTCTTCAACCAATGTAGGATCTCACAATCCACCCGCTTGGGAGCTAGTCTGGCTATGATATCATTTGTAATAGCTCAAACCCATCGCTAGTAGATATTGTTTGTTTTGGTGTGTTACGTATCGCTGTGAGCTTCACGGTTTTAAAGCACATTTGCTAGGGAGAAGTTTCCACACACGCTCAAATCGACGTGGGATCTTGCATAGTCAGTTCTTTGAGAGCTTAGGCTACACTATCTTGACTGGTCTGAATTATCGGTTCATAGTTTGTGGTTAACTCAATGAGTGTTTGGTATCATTAATGCTTGGAGAAATAGTCTACAAATGACCATAAGGAGTCACTTTATGTTAGCTTCATTTGGTTTACTGTTGAAAGAAGTTGGTCCTGAAAGTAAATGGTGAATCAAAAAGCCAAATGTCTTGAAGGTACTCTTTCTTAATGCTGAACTTCTGCAGGTCCCTTCCTTGATTCAATGAAGCACCTTAGAATTACTGTCCTTTTGTCTTCTGTAAACTTCTTCACAATGTTCGTTCAGCTCATAACATGCAATTAAGAGAACTCAAAATGTAACTATTTTAGTGATAAATTGTGAGATCACACGTCAGTGGAGAGGAGAACGAAGCATTGCTTATAAGGATGTAGAAACCTCTCCCTACCGGACGGGTTTTAAAACCTTGAGGGGAAGCCCGGAAGGGAAAGCCCAAAGACGACAATACCTTCTAGCAGTGAGCTTGAGCTGTTACAAATAGTATCAGAGCCAGACATTGGGCGGGGGTGTGCTAGCGAGGACGCTGGTCCCCTAAGGGGGGTAGATTGTGAGATTCAACATCGATCGGAGAGGAGAACGAATCTTTGCTTATAAGGGTGTGGAAACCTCTCTCTACCAAACGCCTTTTAAGACCTTGAGAGAAATCTCGAAAAGGAAAGCCCAAATAGGACAATATCTGCTAGTGGTAAGCTTGAGCTGTTACATAAACTACACTACGGAAGATTGGAAACCATTGGTGTGTATGTTTACCATCCTCAAGTATGGATTGCAGTCTGTCAACCCAGTTAGAGAATGCTTCTTAATGAACTGAGTTTGCTTGTAAATTTAGTGCTGTGTGGAACTGTTAGCTTATGTGTTCTTGAAGATATGATGCTTAGCTCCTATACATGATAGTGATAGTTAGAAATTCGGGACGTATAGCTTCTCCGAAAGCTATCATACTGTAAATTATGTTAAACTGCACGACGTTTGAAGCAGGTCCCATACAAGAGGCTCTACATCTATCCGAACACGAATTCATAAAGATTGTTAAGATAAACTTGATGGCTTCATGGTTTCTGCTGAAGGCTGCTTGTCAAAGAATGCGAGACCAGAACTCAGGAGGCTCGGTAATTTTGTTAACCTCCTTGCTTGGTGCTGAGAGGGGACTGTATCCAGGAGGAGCTGCATATGGATCGTGCTTGGCAGGATTACAGCAGTTAGCTCGGGTAAGTAGCTTTGCATTTTGAAACCATGTGAACATGATCTGTTCTTAAAGTTCCTCGTTATGACATGAATTATGGACTTCTGTAGACTTCAGCTTTGGATGTTGGGAAGTATAAGATCAGGGTCAATGCTATAGCCCGTGGACTGCACCTAGATGATGGATACCCTGTGTCAGTGGGGAAGGAGAGAGCAAAGAAGCTGGTTAATGATGCAGCCCCGCTCGAAAGATGGCTCGATGTTAACGACGATTTAGCTTCGACTGTGATCTATCTAATCAGTGATGGGTCGCGGTACATGACTGGAACGACCATATTTGTTGATGGGGCACAGTCGCTTGTGAGGCCACGGATGCGCTCGTATATGTAGTCGATATGTCTGGTGCACTTGGACATCTTCAGGTCATCAACATTTTGGGTTTCTTTTTTAAATTATGATTAGAAGCATCAATTCAGACAAATATATTATGGTATCATTCTTGTTGACTATTGTCTGTCTTTGTGATGTCCCACGTTAGTTGGG

mRNA sequence

GAAAAAGAGATAATCTGGAGTAATAACTTTTTAACGCAAGAATCATGAGAATATTTTAACGCAACCAATGATGCACACAACGACACTCCTGTCAATCTTGTGACCAGAGTTCTGTGCCAATTTTCTCTTTAATCCTTCATTGTTCTTATCAGGGTTTCATCGGTTTTCGGATCCGAGTTCAATTTCTACCCCCGAATCGAAGCTCCATTTGCTTTGTACACATGGAGAGAGCTACCAAGAACGTGCTGCTTTCTTCTAATGGGGATGAGATATCCAAGAATCTGGCTCTACATCTTGCCAAAAGGGGTTGCAGATTGGTTTTGTTGGGAAATGAGACTGTTCTTCGCAGAACAAGTAAGGAGATAGTTGAATCCGTAAAAGGTGTCCTGCCGATTCAGGTTGTTGGCTTGGACATGGAAGTAGAGGGAGAGGCGGATTTTAGTGAGGCTGTGAACAGGGCTTGCAGTATTTTGGGCGCTTTGGATGCTTTTGTTCATGCTTATTCTTATGATGGTCCCATACAAGAGGCTCTACATCTATCCGAACACGAATTCATAAAGATTGTTAAGATAAACTTGATGGCTTCATGGTTTCTGCTGAAGGCTGCTTGTCAAAGAATGCGAGACCAGAACTCAGGAGGCTCGGTAATTTTGTTAACCTCCTTGCTTGGTGCTGAGAGGGGACTGTATCCAGGAGGAGCTGCATATGGATCGTGCTTGGCAGGATTACAGCAGTTAGCTCGGACTTCAGCTTTGGATGTTGGGAAGTATAAGATCAGGGTCAATGCTATAGCCCGTGGACTGCACCTAGATGATGGATACCCTGTGTCAGTGGGGAAGGAGAGAGCAAAGAAGCTGGTTAATGATGCAGCCCCGCTCGAAAGATGGCTCGATGTTAACGACGATTTAGCTTCGACTGTGATCTATCTAATCAGTGATGGGTCGCGGTACATGACTGGAACGACCATATTTGTTGATGGGGCACAGTCGCTTGTGAGGCCACGGATGCGCTCGTATATGTAGTCGATATGTCTGGTGCACTTGGACATCTTCAGGTCATCAACATTTTGGGTTTCTTTTTTAAATTATGATTAGAAGCATCAATTCAGACAAATATATTATGGTATCATTCTTGTTGACTATTGTCTGTCTTTGTGATGTCCCACGTTAGTTGGG

Coding sequence (CDS)

ATGGAGAGAGCTACCAAGAACGTGCTGCTTTCTTCTAATGGGGATGAGATATCCAAGAATCTGGCTCTACATCTTGCCAAAAGGGGTTGCAGATTGGTTTTGTTGGGAAATGAGACTGTTCTTCGCAGAACAAGTAAGGAGATAGTTGAATCCGTAAAAGGTGTCCTGCCGATTCAGGTTGTTGGCTTGGACATGGAAGTAGAGGGAGAGGCGGATTTTAGTGAGGCTGTGAACAGGGCTTGCAGTATTTTGGGCGCTTTGGATGCTTTTGTTCATGCTTATTCTTATGATGGTCCCATACAAGAGGCTCTACATCTATCCGAACACGAATTCATAAAGATTGTTAAGATAAACTTGATGGCTTCATGGTTTCTGCTGAAGGCTGCTTGTCAAAGAATGCGAGACCAGAACTCAGGAGGCTCGGTAATTTTGTTAACCTCCTTGCTTGGTGCTGAGAGGGGACTGTATCCAGGAGGAGCTGCATATGGATCGTGCTTGGCAGGATTACAGCAGTTAGCTCGGACTTCAGCTTTGGATGTTGGGAAGTATAAGATCAGGGTCAATGCTATAGCCCGTGGACTGCACCTAGATGATGGATACCCTGTGTCAGTGGGGAAGGAGAGAGCAAAGAAGCTGGTTAATGATGCAGCCCCGCTCGAAAGATGGCTCGATGTTAACGACGATTTAGCTTCGACTGTGATCTATCTAATCAGTGATGGGTCGCGGTACATGACTGGAACGACCATATTTGTTGATGGGGCACAGTCGCTTGTGAGGCCACGGATGCGCTCGTATATGTAG

Protein sequence

MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQVVGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLMASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDVGKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDGSRYMTGTTIFVDGAQSLVRPRMRSYM

Homology

[Close]

BLAST of Carg09654 vs. NCBI nr
Match: KAG7017836.1 (fabG [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521.9 bits (1343), Expect = 3.2e-144
Identity = 266/266 (100.00%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV
Sbjct: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM
Sbjct: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV
Sbjct: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of Carg09654 vs. NCBI nr
Match: XP_022934367.1 (uncharacterized protein LOC111441556 [Cucurbita moschata])

HSP 1 Score: 520.4 bits (1339), Expect = 9.2e-144
Identity = 264/266 (99.25%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPI+V
Sbjct: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIEV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           VGLDMEVEGEADFSEAVNRACSILGALDAF+HAYSYDGPIQEALHLSEHEFIKIVKINLM
Sbjct: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFIHAYSYDGPIQEALHLSEHEFIKIVKINLM 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV
Sbjct: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of Carg09654 vs. NCBI nr
Match: KAG6581105.1 (hypothetical protein SDJN03_21107, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 519.6 bits (1337), Expect = 1.6e-143
Identity = 265/266 (99.62%), Postives = 265/266 (99.62%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV
Sbjct: 36  MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 95

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           VGLDMEVEGEADFSEAVNRAC ILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM
Sbjct: 96  VGLDMEVEGEADFSEAVNRACIILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 155

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV
Sbjct: 156 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 215

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG
Sbjct: 216 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 275

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 276 SRYMTGTTIFVDGAQSLVRPRMRSYM 301

[Close]

BLAST of Carg09654 vs. NCBI nr
Match: XP_023526389.1 (uncharacterized protein LOC111789903 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 511.1 bits (1315), Expect = 5.6e-141
Identity = 259/266 (97.37%), Postives = 263/266 (98.87%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPI++
Sbjct: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIEI 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           VGLDME EGEADF+EAVNRACSILG LDAFVHAYSY+GPIQEALHLSEHEF KIVKINLM
Sbjct: 61  VGLDMEEEGEADFTEAVNRACSILGTLDAFVHAYSYEGPIQEALHLSEHEFKKIVKINLM 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV
Sbjct: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of Carg09654 vs. NCBI nr
Match: XP_022983550.1 (uncharacterized protein LOC111482119 [Cucurbita maxima])

HSP 1 Score: 506.9 bits (1304), Expect = 1.1e-139
Identity = 258/266 (96.99%), Postives = 261/266 (98.12%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTS EIVES+KGVLPI+V
Sbjct: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSMEIVESLKGVLPIEV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           VGLDME EGEADFSEAVNRACSILG LDAFVHAYSY+GPIQEALHLSEHEF KIVKINLM
Sbjct: 61  VGLDMEEEGEADFSEAVNRACSILGTLDAFVHAYSYEGPIQEALHLSEHEFKKIVKINLM 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           ASWFLLKAACQRMRDQN GGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV
Sbjct: 121 ASWFLLKAACQRMRDQNLGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of Carg09654 vs. ExPASy Swiss-Prot
Match: P51831 (3-oxoacyl-[acyl-carrier-protein] reductase FabG OS=Bacillus subtilis (strain 168) OX=224308 GN=fabG PE=3 SV=3)

HSP 1 Score: 90.5 bits (223), Expect = 3.1e-17
Identity = 73/255 (28.63%), Postives = 127/255 (49.80%), Query Frame = 0


Query: 6   KNVLLSSNGDEISKNLALHLAKRGCRLVL--LGNETVLRRTSKEIVESVKGVLPIQVVGL 65
           K  +++     I +++AL LAK G  +V+   GNE      + E+V+ +K  +  + + +
Sbjct: 5   KTAIVTGASRGIGRSIALDLAKSGANVVVNYSGNEA----KANEVVDEIKS-MGRKAIAV 64

Query: 66  DMEVEGEADFSEAVNRACSILGALDAFVH--AYSYDGPIQEALHLSEHEFIKIVKINLMA 125
             +V    D    +    S+   +D  V+    + D  I   + + E E+  ++ INL  
Sbjct: 65  KADVSNPEDVQNMIKETLSVFSTIDILVNNAGITRDNLI---MRMKEDEWDDVININLKG 124

Query: 126 SWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDVG 185
            +   KA  ++M  Q S G +I ++S++G      PG A Y +  AG+  L ++SA ++ 
Sbjct: 125 VFNCTKAVTRQMMKQRS-GRIINVSSIVGVSGN--PGQANYVAAKAGVIGLTKSSAKELA 184

Query: 186 KYKIRVNAIARGL---HLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLIS 245
              I VNAIA G     + D     V  E  K++     PL R+ + + D++S V +L S
Sbjct: 185 SRNITVNAIAPGFISTDMTDKLAKDVQDEMLKQI-----PLARFGEPS-DVSSVVTFLAS 242

Query: 246 DGSRYMTGTTIFVDG 254
           +G+RYMTG T+ +DG
Sbjct: 245 EGARYMTGQTLHIDG 242

[Close]

BLAST of Carg09654 vs. ExPASy Swiss-Prot
Match: Q9LBG2 (Levodione reductase OS=Leifsonia aquatica OX=144185 GN=lvr PE=1 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.8e-16
Identity = 69/257 (26.85%), Postives = 117/257 (45.53%), Query Frame = 0


Query: 8   VLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQVVGLDMEV 67
           VL++  G  + +  A+ LA  G +L L+   +     SK  V  ++     +V+    +V
Sbjct: 16  VLITGGGSGLGRATAVRLAAEGAKLSLVDVSSEGLEASKAAV--LETAPDAEVLTTVADV 75

Query: 68  EGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLMASWFLLK 127
             EA     V       G +D F +    +G        +  EF K+V INL   +  L+
Sbjct: 76  SDEAQVEAYVTATTERFGRIDGFFNNAGIEGKQNPTESFTAAEFDKVVSINLRGVFLGLE 135

Query: 128 AACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDVGKYKIRV 187
              + MR+Q SG  +++ T+ +G  RG+    + Y +   G+  L R SA++ G+Y IR+
Sbjct: 136 KVLKIMREQGSG--MVVNTASVGGIRGI-GNQSGYAAAKHGVVGLTRNSAVEYGRYGIRI 195

Query: 188 NAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVN--------DDLASTVIYLISD 247
           NAIA G       P+     +     N     E ++ VN         ++A+ V +L+SD
Sbjct: 196 NAIAPGAIWT---PMVENSMKQLDPENPRKAAEEFIQVNPSKRYGEAPEIAAVVAFLLSD 255

Query: 248 GSRYMTGTTIFVDGAQS 257
            + Y+  T + +DG QS
Sbjct: 256 DASYVNATVVPIDGGQS 264

[Close]

BLAST of Carg09654 vs. ExPASy Swiss-Prot
Match: P9WGQ2 (Uncharacterized oxidoreductase MT1753.1 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT1753.1 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.9e-15
Identity = 68/252 (26.98%), Postives = 115/252 (45.63%), Query Frame = 0


Query: 6   KNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQVVGLDM 65
           K++L++     + +  A  LA  G RL L G        S  + E V G        +  
Sbjct: 23  KSILITGATGSLGRVAARALADAGARLTLAGG------NSAGLAELVNGAGIDDAAVVTC 82

Query: 66  EVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLMASWFL 125
             +  AD  + V  A    G LD  + A S    +     ++  +F  ++  N+  +W +
Sbjct: 83  RPDSLADAQQMVEAALGRYGRLDGVLVA-SGSNHVAPITEMAVEDFDAVMDANVRGAWLV 142

Query: 126 LKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDVGKYKI 185
            +AA + + +Q  GGSV+L++S+ G   G   G +AY    AG   LA+T A + G + I
Sbjct: 143 CRAAGRVLLEQGQGGSVVLVSSVRGG-LGNAAGYSAYCPSKAGTDLLAKTLAAEWGGHGI 202

Query: 186 RVNAIA----RGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDGS 245
           RVNA+A    R    +  +         ++ +    PL R+ +  +D    +IYL+SD S
Sbjct: 203 RVNALAPTVFRSAVTEWMFTDDPKGRATREAMLARIPLRRFAE-PEDFVGALIYLLSDAS 262

Query: 246 RYMTGTTIFVDG 254
            + TG  +++DG
Sbjct: 263 SFYTGQVMYLDG 265

[Close]

BLAST of Carg09654 vs. ExPASy Swiss-Prot
Match: P9WGQ3 (Uncharacterized oxidoreductase Rv1714 OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv1714 PE=3 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 4.9e-15
Identity = 68/252 (26.98%), Postives = 115/252 (45.63%), Query Frame = 0


Query: 6   KNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQVVGLDM 65
           K++L++     + +  A  LA  G RL L G        S  + E V G        +  
Sbjct: 23  KSILITGATGSLGRVAARALADAGARLTLAGG------NSAGLAELVNGAGIDDAAVVTC 82

Query: 66  EVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLMASWFL 125
             +  AD  + V  A    G LD  + A S    +     ++  +F  ++  N+  +W +
Sbjct: 83  RPDSLADAQQMVEAALGRYGRLDGVLVA-SGSNHVAPITEMAVEDFDAVMDANVRGAWLV 142

Query: 126 LKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDVGKYKI 185
            +AA + + +Q  GGSV+L++S+ G   G   G +AY    AG   LA+T A + G + I
Sbjct: 143 CRAAGRVLLEQGQGGSVVLVSSVRGG-LGNAAGYSAYCPSKAGTDLLAKTLAAEWGGHGI 202

Query: 186 RVNAIA----RGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDGS 245
           RVNA+A    R    +  +         ++ +    PL R+ +  +D    +IYL+SD S
Sbjct: 203 RVNALAPTVFRSAVTEWMFTDDPKGRATREAMLARIPLRRFAE-PEDFVGALIYLLSDAS 262

Query: 246 RYMTGTTIFVDG 254
            + TG  +++DG
Sbjct: 263 SFYTGQVMYLDG 265

[Close]

BLAST of Carg09654 vs. ExPASy Swiss-Prot
Match: Q2UEK6 (Short chain dehydrogenase/reductase astE OS=Aspergillus oryzae (strain ATCC 42149 / RIB 40) OX=510516 GN=astEA PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 1.9e-14
Identity = 73/256 (28.52%), Postives = 117/256 (45.70%), Query Frame = 0


Query: 6   KNVLLSSNGDEISKNLALHLAKRGCRLVLL-GNETVLRRTSKEIVESVKGVLPIQVVGLD 65
           K  +++  G  I +  AL LA  G  +V+   NET    T KE    V      + + + 
Sbjct: 13  KVAIVTGAGSGIGRETALCLANAGANVVVAEANET----TGKETAAKVSAQTGSRGLFIL 72

Query: 66  MEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLMASWF 125
            +V         V       G LD  V+  +          L E  + KI+ +NL+   F
Sbjct: 73  TDVSRSESVQAMVIATIEAFGRLDIAVNNAALHPDASPIAELHEDHWQKIIGVNLVGVAF 132

Query: 126 LLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDVGKYK 185
            LK   Q+M  Q  GGS+I ++S            +AY +   G+  L +T+A++ G++ 
Sbjct: 133 CLKWELQQMIQQGGGGSIINISS--ATINRPQEKMSAYIAAKHGITGLTQTAAVENGRHG 192

Query: 186 IRVNAIARGLHLDDGYPVSVGKERAKKLVNDAA--PLERWLDVNDDLASTVIYLISDGSR 245
           IRVNA+A G    D   ++  +E      N+AA   L +     +++A +V++L SD + 
Sbjct: 193 IRVNALAPGGVATD-LTMATMQELGLTEENEAARSSLFKRFAKPEEIAQSVLWLASDAAS 252

Query: 246 YMTGTTIFVDGAQSLV 259
           Y+TG TI VD   SL+
Sbjct: 253 YVTGATIAVDSGLSLI 261

[Close]

BLAST of Carg09654 vs. ExPASy TrEMBL
Match: A0A6J1F2J2 (uncharacterized protein LOC111441556 OS=Cucurbita moschata OX=3662 GN=LOC111441556 PE=4 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 4.5e-144
Identity = 264/266 (99.25%), Postives = 266/266 (100.00%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPI+V
Sbjct: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIEV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           VGLDMEVEGEADFSEAVNRACSILGALDAF+HAYSYDGPIQEALHLSEHEFIKIVKINLM
Sbjct: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFIHAYSYDGPIQEALHLSEHEFIKIVKINLM 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV
Sbjct: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of Carg09654 vs. ExPASy TrEMBL
Match: A0A6J1J660 (uncharacterized protein LOC111482119 OS=Cucurbita maxima OX=3661 GN=LOC111482119 PE=4 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.1e-140
Identity = 258/266 (96.99%), Postives = 261/266 (98.12%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTS EIVES+KGVLPI+V
Sbjct: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSMEIVESLKGVLPIEV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           VGLDME EGEADFSEAVNRACSILG LDAFVHAYSY+GPIQEALHLSEHEF KIVKINLM
Sbjct: 61  VGLDMEEEGEADFSEAVNRACSILGTLDAFVHAYSYEGPIQEALHLSEHEFKKIVKINLM 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           ASWFLLKAACQRMRDQN GGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV
Sbjct: 121 ASWFLLKAACQRMRDQNLGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of Carg09654 vs. ExPASy TrEMBL
Match: Q2PZA4 (Putative alcohol dehydrogenases OS=Cucumis melo OX=3656 GN=ADH2 PE=2 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 2.3e-124
Identity = 231/266 (86.84%), Postives = 244/266 (91.73%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           ME ATKNVLLSS GDEISKNLALHLA+RGCRLVL+GNE VL+  SK I ES+KGVLPI+V
Sbjct: 1   MEGATKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           VGLDME E EA F EAVNRACS+LG LDAFVHAYSY+GPIQ+AL LSE EF KIVKINLM
Sbjct: 61  VGLDMEEEREAAFDEAVNRACSVLGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           ASWFL+KA C+RMRDQ SGGSVI LT+L+GAERGLYPGGAAYGSC AGLQQLARTSALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLD+GYPVSVGKERAKKLV DAA LERWLDV DDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDNGYPVSVGKERAKKLVKDAAKLERWLDVKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of Carg09654 vs. ExPASy TrEMBL
Match: A0A0A0L8L9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G566330 PE=4 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 1.1e-123
Identity = 229/266 (86.09%), Postives = 243/266 (91.35%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           ME +TKNVLLSS GDEISKNLALHLA+RGCRLVL+GNE VL+  SK I ES+KGVLPI+V
Sbjct: 1   MEGSTKNVLLSSGGDEISKNLALHLARRGCRLVLIGNECVLQSMSKMIAESLKGVLPIEV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           VG+DME E EA F EAVNRAC ILG LDAFVHAYSY+GPIQ+AL LSE EF KIVKINLM
Sbjct: 61  VGVDMEEEREAAFDEAVNRACGILGTLDAFVHAYSYEGPIQDALQLSEEEFKKIVKINLM 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           ASWFL+KA C+RMRDQ SGGSVI LT+L+GAERGLYPGGAAYGSC AGLQQLARTSALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSVIFLTTLIGAERGLYPGGAAYGSCSAGLQQLARTSALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GKYKIRVNAIARGLHLDDGYPVSVGKERA+K V DAAPLERWLDV DDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAEKQVKDAAPLERWLDVKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of Carg09654 vs. ExPASy TrEMBL
Match: A0A6J1EJP8 (carbonyl reductase [NADPH] 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435110 PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 6.9e-121
Identity = 221/266 (83.08%), Postives = 241/266 (90.60%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           ME ATKNVLLSSNGDEISKNLA+HLAKRGCRLVL+GNETVLR   + IVES+KG LPI V
Sbjct: 1   MEGATKNVLLSSNGDEISKNLAIHLAKRGCRLVLVGNETVLRSMREGIVESLKGALPIGV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           VGLDME E EA F EAV+ ACS+LG LDAFVHAYSY+GPIQE L LSE EF KIVKIN+M
Sbjct: 61  VGLDMEGEREAAFDEAVSGACSVLGTLDAFVHAYSYEGPIQEVLQLSEEEFKKIVKINMM 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           ASWFL+KA C+RMRDQ SGGS++ L+SL+G+ERGLYPG AA+GSC AGLQQLART+ALDV
Sbjct: 121 ASWFLMKAVCRRMRDQKSGGSIVFLSSLIGSERGLYPGAAAFGSCSAGLQQLARTAALDV 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GKYKIRVNAI+RGLHLDDGYP+SVG ERAKKLV DAAPLERWLD+ DDLASTVIYLISDG
Sbjct: 181 GKYKIRVNAISRGLHLDDGYPISVGTERAKKLVKDAAPLERWLDIKDDLASTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SRYMTGTTIFVDGAQSLVRPRMRSYM
Sbjct: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 266

[Close]

BLAST of Carg09654 vs. TAIR 10
Match: AT3G01980.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 4.6e-85
Identity = 149/266 (56.02%), Postives = 195/266 (73.31%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           ME   K VL++SNGDE+S+N+A HLAK GC+LV++GNE  LR    +I +S++G  P  V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           + LDME + E  F  AV +A  + G  DAF+++Y+Y G +Q+ L +S+ EF +I KINL 
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGKVQDILQVSQDEFHRITKINLT 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           A WFLLKA   RM+D  SGGS++ + ++   ER LYPG  AY S  A + QL R SA+ +
Sbjct: 121 APWFLLKAVATRMKDHGSGGSIVFMATIASGERALYPGADAYASTSAAIHQLVRASAMSL 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GK+KIRVN I+RGLHLDD Y  SVG++RA+KLV DAAPL +WL+ + DL STVIYLISDG
Sbjct: 181 GKHKIRVNMISRGLHLDDEYTASVGRDRAQKLVKDAAPLGQWLNPDTDLYSTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SR+MTGTT+ VDGAQSL RPR++SYM
Sbjct: 241 SRFMTGTTVLVDGAQSLTRPRLKSYM 266

[Close]

BLAST of Carg09654 vs. TAIR 10
Match: AT3G01980.4 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 312.0 bits (798), Expect = 4.6e-85
Identity = 149/266 (56.02%), Postives = 195/266 (73.31%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           ME   K VL++SNGDE+S+N+A HLAK GC+LV++GNE  LR    +I +S++G  P  V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 120
           + LDME + E  F  AV +A  + G  DAF+++Y+Y G +Q+ L +S+ EF +I KINL 
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGKVQDILQVSQDEFHRITKINLT 120

Query: 121 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 180
           A WFLLKA   RM+D  SGGS++ + ++   ER LYPG  AY S  A + QL R SA+ +
Sbjct: 121 APWFLLKAVATRMKDHGSGGSIVFMATIASGERALYPGADAYASTSAAIHQLVRASAMSL 180

Query: 181 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 240
           GK+KIRVN I+RGLHLDD Y  SVG++RA+KLV DAAPL +WL+ + DL STVIYLISDG
Sbjct: 181 GKHKIRVNMISRGLHLDDEYTASVGRDRAQKLVKDAAPLGQWLNPDTDLYSTVIYLISDG 240

Query: 241 SRYMTGTTIFVDGAQSLVRPRMRSYM 267
           SR+MTGTT+ VDGAQSL RPR++SYM
Sbjct: 241 SRFMTGTTVLVDGAQSLTRPRLKSYM 266

[Close]

BLAST of Carg09654 vs. TAIR 10
Match: AT3G01980.3 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 296.2 bits (757), Expect = 2.6e-80
Identity = 149/296 (50.34%), Postives = 195/296 (65.88%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           ME   K VL++SNGDE+S+N+A HLAK GC+LV++GNE  LR    +I +S++G  P  V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYD----------------------- 120
           + LDME + E  F  AV +A  + G  DAF+++Y+Y                        
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGLICFLFFTTLPLMLLCVDHSFI 120

Query: 121 -------GPIQEALHLSEHEFIKIVKINLMASWFLLKAACQRMRDQNSGGSVILLTSLLG 180
                  G +Q+ L +S+ EF +I KINL A WFLLKA   RM+D  SGGS++ + ++  
Sbjct: 121 QQSFFLAGKVQDILQVSQDEFHRITKINLTAPWFLLKAVATRMKDHGSGGSIVFMATIAS 180

Query: 181 AERGLYPGGAAYGSCLAGLQQLARTSALDVGKYKIRVNAIARGLHLDDGYPVSVGKERAK 240
            ER LYPG  AY S  A + QL R SA+ +GK+KIRVN I+RGLHLDD Y  SVG++RA+
Sbjct: 181 GERALYPGADAYASTSAAIHQLVRASAMSLGKHKIRVNMISRGLHLDDEYTASVGRDRAQ 240

Query: 241 KLVNDAAPLERWLDVNDDLASTVIYLISDGSRYMTGTTIFVDGAQSLVRPRMRSYM 267
           KLV DAAPL +WL+ + DL STVIYLISDGSR+MTGTT+ VDGAQSL RPR++SYM
Sbjct: 241 KLVKDAAPLGQWLNPDTDLYSTVIYLISDGSRFMTGTTVLVDGAQSLTRPRLKSYM 296

[Close]

BLAST of Carg09654 vs. TAIR 10
Match: AT3G01980.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 163.3 bits (412), Expect = 2.7e-40
Identity = 85/208 (40.87%), Postives = 117/208 (56.25%), Query Frame = 0


Query: 1   MERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQV 60
           ME   K VL++SNGDE+S+N+A HLAK GC+LV++GNE  LR    +I +S++G  P  V
Sbjct: 1   MENPAKRVLMTSNGDEVSRNIAFHLAKHGCKLVMMGNEGSLRSIVDKIRDSIEGAFPADV 60

Query: 61  VGLDMEVEGEADFSEAVNRACSILGALDAFVHAYSYD----------------------- 120
           + LDME + E  F  AV +A  + G  DAF+++Y+Y                        
Sbjct: 61  IALDMESDSEVAFHAAVQKAWELSGHFDAFLNSYTYQGLICFLFFTTLPLMLLCVDHSFI 120

Query: 121 -------GPIQEALHLSEHEFIKIVKINLMASWFLLKAACQRMRDQNSGGSVILLTSLLG 179
                  G +Q+ L +S+ EF +I KINL A WFLLKA   RM+D  SGGS++ + ++  
Sbjct: 121 QQSFFLAGKVQDILQVSQDEFHRITKINLTAPWFLLKAVATRMKDHGSGGSIVFMATIAS 180

[Close]

BLAST of Carg09654 vs. TAIR 10
Match: AT1G63380.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 149.1 bits (375), Expect = 5.2e-36
Identity = 89/257 (34.63%), Postives = 130/257 (50.58%), Query Frame = 0


Query: 2   ERATKNVLLSSNGDEISKNLALHLAKRGCRLVLLGNETVLRRTSKEIVESVKGVLPIQVV 61
           E   K VL++     I + + L L K GC++V          +    + S  G + +Q V
Sbjct: 16  ELKDKVVLVTGASSGIGREICLDLCKAGCKIVAAARRVDRLNSLCSEINSF-GAIGVQAV 75

Query: 62  GLDMEVEGEAD-FSEAVNRACSILGALDAFVHAYSYDGPIQEALHLSEHEFIKIVKINLM 121
            L+++V  EAD   +AV  A    G +D  ++     G ++ +L LSE E+ K+ + NL 
Sbjct: 76  ALELDVSSEADTIRKAVKEAWETFGKIDVLINNAGIRGNVKSSLDLSEEEWDKVFRTNLT 135

Query: 122 ASWFLLKAACQRMRDQNSGGSVILLTSLLGAERGLYPGGAAYGSCLAGLQQLARTSALDV 181
            SW + K  C  MRD   GGSVI ++S+ G  RGL  GG AY     G+  + R  A+++
Sbjct: 136 GSWLISKYVCLLMRDAERGGSVINVSSISGLHRGLLRGGLAYACSKGGVDTMTRMMAIEL 195

Query: 182 GKYKIRVNAIARGLHLDDGYPVSVGKERAKKLVNDAAPLERWLDVNDDLASTVIYLISDG 241
             YKIRVN+IA G+   +       KE  KK+     PL+    V+  L S V YLI D 
Sbjct: 196 AVYKIRVNSIAPGIFRSEITQGLFQKEWLKKVTEKVVPLKMQQTVDPGLTSLVRYLIHDS 255

Query: 242 SRYMTGTTIFVDGAQSL 258
           S+Y+TG T  VD   +L
Sbjct: 256 SQYVTGNTYIVDSGTTL 271

The following BLAST results are available for this feature:

BLAST of Carg09654 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017836.1	3.2e-144	100.00	fabG [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022934367.1	9.2e-144	99.25	uncharacterized protein LOC111441556 [Cucurbita moschata]	[more]
KAG6581105.1	1.6e-143	99.62	hypothetical protein SDJN03_21107, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023526389.1	5.6e-141	97.37	uncharacterized protein LOC111789903 [Cucurbita pepo subsp. pepo]	[more]
XP_022983550.1	1.1e-139	96.99	uncharacterized protein LOC111482119 [Cucurbita maxima]	[more]

BLAST of Carg09654 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P51831	3.1e-17	28.63	3-oxoacyl-[acyl-carrier-protein] reductase FabG OS=Bacillus subtilis (strain 168...	[more]
Q9LBG2	5.8e-16	26.85	Levodione reductase OS=Leifsonia aquatica OX=144185 GN=lvr PE=1 SV=1	[more]
P9WGQ2	4.9e-15	26.98	Uncharacterized oxidoreductase MT1753.1 OS=Mycobacterium tuberculosis (strain CD...	[more]
P9WGQ3	4.9e-15	26.98	Uncharacterized oxidoreductase Rv1714 OS=Mycobacterium tuberculosis (strain ATCC...	[more]
Q2UEK6	1.9e-14	28.52	Short chain dehydrogenase/reductase astE OS=Aspergillus oryzae (strain ATCC 4214...	[more]

BLAST of Carg09654 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F2J2	4.5e-144	99.25	uncharacterized protein LOC111441556 OS=Cucurbita moschata OX=3662 GN=LOC1114415...	[more]
A0A6J1J660	5.1e-140	96.99	uncharacterized protein LOC111482119 OS=Cucurbita maxima OX=3661 GN=LOC111482119...	[more]
Q2PZA4	2.3e-124	86.84	Putative alcohol dehydrogenases OS=Cucumis melo OX=3656 GN=ADH2 PE=2 SV=1	[more]
A0A0A0L8L9	1.1e-123	86.09	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G566330 PE=4 SV=1	[more]
A0A6J1EJP8	6.9e-121	83.08	carbonyl reductase [NADPH] 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435110 ...	[more]

BLAST of Carg09654 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G01980.1	4.6e-85	56.02	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G01980.4	4.6e-85	56.02	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G01980.3	2.6e-80	50.34	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G01980.2	2.7e-40	40.87	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G63380.1	5.2e-36	34.63	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 162..181 score: 24.24 coord: 183..200 score: 31.84 coord: 219..239 score: 21.25 coord: 134..150 score: 25.59
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 17..253 e-value: 9.9E-37 score: 126.7
None	No IPR available	GENE3D	3.40.50.720		coord: 4..263 e-value: 6.7E-40 score: 138.8
None	No IPR available	PANTHER	PTHR44375:SF6	F28J7.36 PROTEIN	coord: 1..266
None	No IPR available	PANTHER	PTHR44375	BETA-KETOACYL-ACP REDUCTASE-LIKE PROTEIN-RELATED	coord: 1..266
None	No IPR available	CDD	cd05233	SDR_c	coord: 21..252 e-value: 3.09259E-38 score: 132.022
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 14..257

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg09654-RA	Carg09654-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004090	carbonyl reductase (NADPH) activity
molecular_function	GO:0016491	oxidoreductase activity