Carg09569 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09569
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionlate embryogenesis abundant protein 1-like
LocationCarg_Chr17: 6906099 .. 6906482 (+)
RNA-Seq ExpressionCarg09569
SyntenyCarg09569
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAGCCAACAGGATTTGAGCTTCAAGGCTGGGGAGACTGTTGGGCAAGCTCAGGTCAAGAAGGATGAGGTTACGAGCCAAGCAACATCCACCCAGGATGCCCAATCCGCCGCCCAAACTGCCACCGACCTGAAGGACCAAGCTGCCAGCTTCCTCCAACAGGCAATTACTTGTCATTTTCACTACTTTCACTGACAAGAATGGCTGTGTGTTTTTTTTTTTTTAATTTGTTGTGTGTTTGTGTAGACTGGAGAGCAAGTGAAGAGCATGGCACAAGGAGCAGCTGAGGCAGTGAAGAACACACTTGGGATGAAGACTGACACTGATACCACCACCTCACCCAACAACAACCCTACCAACAATCCTTCCCCTACAATTTAA

mRNA sequence

ATGGCAAGCCAACAGGATTTGAGCTTCAAGGCTGGGGAGACTGTTGGGCAAGCTCAGGTCAAGAAGGATGAGACTGGAGAGCAAGTGAAGAGCATGGCACAAGGAGCAGCTGAGGCAGTGAAGAACACACTTGGGATGAAGACTGACACTGATACCACCACCTCACCCAACAACAACCCTACCAACAATCCTTCCCCTACAATTTAA

Coding sequence (CDS)

ATGGCAAGCCAACAGGATTTGAGCTTCAAGGCTGGGGAGACTGTTGGGCAAGCTCAGGTCAAGAAGGATGAGACTGGAGAGCAAGTGAAGAGCATGGCACAAGGAGCAGCTGAGGCAGTGAAGAACACACTTGGGATGAAGACTGACACTGATACCACCACCTCACCCAACAACAACCCTACCAACAATCCTTCCCCTACAATTTAA

Protein sequence

MASQQDLSFKAGETVGQAQVKKDETGEQVKSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPTI
Homology
BLAST of Carg09569 vs. NCBI nr
Match: KAG7014122.1 (hypothetical protein SDJN02_24295, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 125.6 bits (314), Expect = 1.7e-25
Identity = 68/68 (100.00%), Postives = 68/68 (100.00%), Query Frame = 0

Query: 1  MASQQDLSFKAGETVGQAQVKKDETGEQVKSMAQGAAEAVKNTLGMKTDTDTTTSPNNNP 60
          MASQQDLSFKAGETVGQAQVKKDETGEQVKSMAQGAAEAVKNTLGMKTDTDTTTSPNNNP
Sbjct: 1  MASQQDLSFKAGETVGQAQVKKDETGEQVKSMAQGAAEAVKNTLGMKTDTDTTTSPNNNP 60

Query: 61 TNNPSPTI 69
          TNNPSPTI
Sbjct: 61 TNNPSPTI 68

BLAST of Carg09569 vs. NCBI nr
Match: KAG6575581.1 (hypothetical protein SDJN03_26220, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 109.4 bits (272), Expect = 1.2e-20
Identity = 68/99 (68.69%), Postives = 68/99 (68.69%), Query Frame = 0

Query: 1  MASQQDLSFKAGETVGQAQVKKDE-------------------------------TGEQV 60
          MASQQDLSFKAGETVGQAQVKKDE                               TGEQV
Sbjct: 1  MASQQDLSFKAGETVGQAQVKKDEVMSQATSTQDAQSAAQTATDLKDQAASFLQQTGEQV 60

Query: 61 KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPTI 69
          KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPTI
Sbjct: 61 KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPTI 99

BLAST of Carg09569 vs. NCBI nr
Match: XP_022954121.1 (late embryogenesis abundant protein 1-like [Cucurbita moschata])

HSP 1 Score: 107.5 bits (267), Expect = 4.7e-20
Identity = 67/98 (68.37%), Postives = 67/98 (68.37%), Query Frame = 0

Query: 1  MASQQDLSFKAGETVGQAQVKKDE-------------------------------TGEQV 60
          MASQQDLSFKAGETVGQAQVKKDE                               TGEQV
Sbjct: 1  MASQQDLSFKAGETVGQAQVKKDEVMSQATSTQDAQSAAQTATDLKDQAASFLQQTGEQV 60

Query: 61 KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPT 68
          KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPT
Sbjct: 61 KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPT 98

BLAST of Carg09569 vs. NCBI nr
Match: XP_022991676.1 (late embryogenesis abundant protein 1-like [Cucurbita maxima])

HSP 1 Score: 102.4 bits (254), Expect = 1.5e-18
Identity = 63/99 (63.64%), Postives = 66/99 (66.67%), Query Frame = 0

Query: 1  MASQQDLSFKAGETVGQAQVKKD-------------------------------ETGEQV 60
          MASQQDLS+KAGETVGQAQ  KD                               +TGEQV
Sbjct: 1  MASQQDLSYKAGETVGQAQASKDDIMSQLTSTQDAQSAAQTATDLKDQAANFLQQTGEQV 60

Query: 61 KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPTI 69
          KSMAQGAAEAVKNTLGMKTDTDTTT+PNNNPTNNPSPTI
Sbjct: 61 KSMAQGAAEAVKNTLGMKTDTDTTTAPNNNPTNNPSPTI 99

BLAST of Carg09569 vs. NCBI nr
Match: KAG7025770.1 (hypothetical protein SDJN02_12268, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 93.2 bits (230), Expect = 9.3e-16
Identity = 53/69 (76.81%), Postives = 58/69 (84.06%), Query Frame = 0

Query: 1  MASQQDLSFKAGETVGQAQVKKDETGEQVKSMAQGAAEAVKNTLGMKTDTDTTTSPN-NN 60
          MASQQD+S   GE VGQAQVKKDETGEQVK+MAQGAAEAVKNTLGM TD+ +  + N NN
Sbjct: 1  MASQQDISHSVGEAVGQAQVKKDETGEQVKNMAQGAAEAVKNTLGMNTDSSSNAAANTNN 60

Query: 61 PTNNPSPTI 69
          PTN PSPTI
Sbjct: 61 PTNTPSPTI 69

BLAST of Carg09569 vs. ExPASy TrEMBL
Match: A0A6J1GQ03 (late embryogenesis abundant protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC111456481 PE=4 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 2.3e-20
Identity = 67/98 (68.37%), Postives = 67/98 (68.37%), Query Frame = 0

Query: 1  MASQQDLSFKAGETVGQAQVKKDE-------------------------------TGEQV 60
          MASQQDLSFKAGETVGQAQVKKDE                               TGEQV
Sbjct: 1  MASQQDLSFKAGETVGQAQVKKDEVMSQATSTQDAQSAAQTATDLKDQAASFLQQTGEQV 60

Query: 61 KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPT 68
          KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPT
Sbjct: 61 KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPT 98

BLAST of Carg09569 vs. ExPASy TrEMBL
Match: A0A6J1JRF1 (late embryogenesis abundant protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111488219 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 7.4e-19
Identity = 63/99 (63.64%), Postives = 66/99 (66.67%), Query Frame = 0

Query: 1  MASQQDLSFKAGETVGQAQVKKD-------------------------------ETGEQV 60
          MASQQDLS+KAGETVGQAQ  KD                               +TGEQV
Sbjct: 1  MASQQDLSYKAGETVGQAQASKDDIMSQLTSTQDAQSAAQTATDLKDQAANFLQQTGEQV 60

Query: 61 KSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNNPSPTI 69
          KSMAQGAAEAVKNTLGMKTDTDTTT+PNNNPTNNPSPTI
Sbjct: 61 KSMAQGAAEAVKNTLGMKTDTDTTTAPNNNPTNNPSPTI 99

BLAST of Carg09569 vs. ExPASy TrEMBL
Match: A0A5D3CF20 (Late embryogenesis abundant protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold459G002210 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 1.7e-10
Identity = 57/109 (52.29%), Postives = 61/109 (55.96%), Query Frame = 0

Query: 1   MASQQD-LSFKAGETVGQAQVKKDE-----------------------------TGEQVK 60
           MAS QD LS KAGETVGQAQVKKDE                             TGEQVK
Sbjct: 1   MASHQDNLSHKAGETVGQAQVKKDEMMNQPTAHDQSATQTASDLKDQAANFLQQTGEQVK 60

Query: 61  SMAQGAAEAVKNTLGMKTD-----------TDTTTSPNNNPTNNPSPTI 69
           +MAQGAAEAVKNTLGM TD            + TT+P+NNPTNNPS  I
Sbjct: 61  NMAQGAAEAVKNTLGMNTDNTSNPNSNNPTNNPTTNPSNNPTNNPSTRI 109

BLAST of Carg09569 vs. ExPASy TrEMBL
Match: A0A1S3CF46 (late embryogenesis abundant protein 1 OS=Cucumis melo OX=3656 GN=LOC103499779 PE=4 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 3.7e-10
Identity = 55/96 (57.29%), Postives = 60/96 (62.50%), Query Frame = 0

Query: 1  MASQQD-LSFKAGETVGQAQVKKDE-----------------------------TGEQVK 60
          MAS QD LS KAGETVGQAQVKKDE                             TGEQVK
Sbjct: 1  MASHQDNLSHKAGETVGQAQVKKDEMMNQPTAHDQSATQTASDLKDQAANFLQQTGEQVK 60

Query: 61 SMAQGAAEAVKNTLGMKTDTDTTTSPN-NNPTNNPS 66
          +MAQGAAEAVKNTLGM  +TD T++PN NNPTNNP+
Sbjct: 61 NMAQGAAEAVKNTLGM--NTDNTSNPNSNNPTNNPT 94

BLAST of Carg09569 vs. ExPASy TrEMBL
Match: A0A6J1KJU5 (late embryogenesis abundant protein 1 OS=Cucurbita maxima OX=3661 GN=LOC111494669 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 6.3e-10
Identity = 53/111 (47.75%), Postives = 58/111 (52.25%), Query Frame = 0

Query: 1   MASQQDLSFKAGETVGQAQVKKDE------------------------------------ 60
           MASQQD+S   GE VGQAQVKKDE                                    
Sbjct: 1   MASQQDISHSVGEVVGQAQVKKDEVMNQAANAAQEAKDSASTTLDQSSAAQTASDLKDQA 60

Query: 61  ------TGEQVKSMAQGAAEAVKNTLGMKTDTDTTTSPN-NNPTNNPSPTI 69
                 TGEQVK+MAQGAAEAVKNTLGM TD+ +  + N NNPTN PSPTI
Sbjct: 61  ANFLQQTGEQVKNMAQGAAEAVKNTLGMNTDSSSNAAANTNNPTNTPSPTI 111

BLAST of Carg09569 vs. TAIR 10
Match: AT1G52680.1 (late embryogenesis abundant protein-related / LEA protein-related )

HSP 1 Score: 42.7 bits (99), Expect = 1.3e-04
Identity = 37/107 (34.58%), Postives = 46/107 (42.99%), Query Frame = 0

Query: 1   MASQQDLSFKAGETVGQAQVKKDE------------------------------------ 60
           M+S Q LS  AGE  GQ Q+KK+E                                    
Sbjct: 1   MSSSQQLSHSAGEVTGQVQLKKEEYLNNVSHAMNQNADHHTHSQSQLHSEHDQNNPSLIS 60

Query: 61  --------TGEQVKSMAQGAAEAVKNTLGMKTDTDTTTSPNNNPTNN 64
                   TG QVK+MAQGAA+AVKNTLGM   T++ +SP     +N
Sbjct: 61  QASSVIQQTGGQVKNMAQGAADAVKNTLGMSPATNSPSSPAGTTRSN 107

BLAST of Carg09569 vs. TAIR 10
Match: AT1G15415.1 (The protein encoded by this gene was identified as a part of pollen proteome by mass spec analysis. It has weak homology to LEA (late embryo abundant) proteins. Encodes protein phosphatase 2A (PP2A) B'gamma subunit. Targeted to nucleus and cytosol. )

HSP 1 Score: 41.2 bits (95), Expect = 3.9e-04
Identity = 36/88 (40.91%), Postives = 47/88 (53.41%), Query Frame = 0

Query: 1  MASQQD--LSFKAGETVGQAQVKKD-------------ETGEQVKSMAQGAAEAVKNTLG 60
          MAS QD   S +AG+ VGQA VK++             + GE+VKSMA  A+EAVKN LG
Sbjct: 1  MASGQDEEFSVRAGQIVGQAHVKENDCNNSSQASGFLQQKGEKVKSMAHDASEAVKNKLG 60

Query: 61 MKTDTDTTTSPNN-----NPTNNPSPTI 69
          +  D +      N     NP N  SP++
Sbjct: 61 INNDNNEEYKNKNPLDKKNPNNTTSPSM 88

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7014122.11.7e-25100.00hypothetical protein SDJN02_24295, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6575581.11.2e-2068.69hypothetical protein SDJN03_26220, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022954121.14.7e-2068.37late embryogenesis abundant protein 1-like [Cucurbita moschata][more]
XP_022991676.11.5e-1863.64late embryogenesis abundant protein 1-like [Cucurbita maxima][more]
KAG7025770.19.3e-1676.81hypothetical protein SDJN02_12268, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1GQ032.3e-2068.37late embryogenesis abundant protein 1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1JRF17.4e-1963.64late embryogenesis abundant protein 1-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5D3CF201.7e-1052.29Late embryogenesis abundant protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3CF463.7e-1057.29late embryogenesis abundant protein 1 OS=Cucumis melo OX=3656 GN=LOC103499779 PE... [more]
A0A6J1KJU56.3e-1047.75late embryogenesis abundant protein 1 OS=Cucurbita maxima OX=3661 GN=LOC11149466... [more]
Match NameE-valueIdentityDescription
AT1G52680.11.3e-0434.58late embryogenesis abundant protein-related / LEA protein-related [more]
AT1G15415.13.9e-0440.91The protein encoded by this gene was identified as a part of pollen proteome by... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..62
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..68
NoneNo IPR availablePANTHERPTHR34191:SF9F6D8.10coord: 1..25
NoneNo IPR availablePANTHERPTHR34191:SF9F6D8.10coord: 23..51
IPR039624Stress-induced protein KIN1/KIN2PANTHERPTHR34191LATE EMBRYOGENESIS ABUNDANT PROTEIN (LEA) FAMILY PROTEINcoord: 1..25
IPR039624Stress-induced protein KIN1/KIN2PANTHERPTHR34191LATE EMBRYOGENESIS ABUNDANT PROTEIN (LEA) FAMILY PROTEINcoord: 23..51

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09569-RACarg09569-RAmRNA