Carg09387 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg09387
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionComplex 1 LYR protein
LocationCarg_Chr05: 1498197 .. 1498673 (-)
RNA-Seq ExpressionCarg09387
SyntenyCarg09387
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCATGAGATTGGTAGAAAAATGTGTTAAGCTGTCAACTCACAAGAACTTTCTGGCTTGCAATAAAATTGGTTACCACCCTCGAGCTCTGCATAATGGTCCAGACACCATTGATGAACTCTTAGACAGACATGTGGTAAAGAAAGAAAAGTCCTTTGATGACGATGAAGAAGAATTGATAACTCAGCGACGCCTCACCAGCACTCGACGTGAGGCTCTAAGTCTCTACAGGGACATTCTCCGTGCAACTCGATTCTTTATGTGGCCAGATTCTCGAGGTGTCTTGTGGCGAGATGTTCTCAGGCAGAATGCACGGAAGGAGTTTGAGGACGCCCGCTTTGAGACTGATCCAGAAATTGTGACCAGACTTCTCATTGGTGGGCGTGATGCAGTGCAGTCGGCTCTTGACAAGCTTGCTGAGAAGCAGAGGCAGGAGATTGATAAGAAACAAAGTGGTGGTCGAGATCGACCCTGA

mRNA sequence

ATGAGCATGAGATTGGTAGAAAAATGTGTTAAGCTGTCAACTCACAAGAACTTTCTGGCTTGCAATAAAATTGGTTACCACCCTCGAGCTCTGCATAATGGTCCAGACACCATTGATGAACTCTTAGACAGACATGTGGTAAAGAAAGAAAAGTCCTTTGATGACGATGAAGAAGAATTGATAACTCAGCGACGCCTCACCAGCACTCGACGTGAGGCTCTAAGTCTCTACAGGGACATTCTCCGTGCAACTCGATTCTTTATGTGGCCAGATTCTCGAGGTGTCTTGTGGCGAGATGTTCTCAGGCAGAATGCACGGAAGGAGTTTGAGGACGCCCGCTTTGAGACTGATCCAGAAATTGTGACCAGACTTCTCATTGGTGGGCGTGATGCAGTGCAGTCGGCTCTTGACAAGCTTGCTGAGAAGCAGAGGCAGGAGATTGATAAGAAACAAAGTGGTGGTCGAGATCGACCCTGA

Coding sequence (CDS)

ATGAGCATGAGATTGGTAGAAAAATGTGTTAAGCTGTCAACTCACAAGAACTTTCTGGCTTGCAATAAAATTGGTTACCACCCTCGAGCTCTGCATAATGGTCCAGACACCATTGATGAACTCTTAGACAGACATGTGGTAAAGAAAGAAAAGTCCTTTGATGACGATGAAGAAGAATTGATAACTCAGCGACGCCTCACCAGCACTCGACGTGAGGCTCTAAGTCTCTACAGGGACATTCTCCGTGCAACTCGATTCTTTATGTGGCCAGATTCTCGAGGTGTCTTGTGGCGAGATGTTCTCAGGCAGAATGCACGGAAGGAGTTTGAGGACGCCCGCTTTGAGACTGATCCAGAAATTGTGACCAGACTTCTCATTGGTGGGCGTGATGCAGTGCAGTCGGCTCTTGACAAGCTTGCTGAGAAGCAGAGGCAGGAGATTGATAAGAAACAAAGTGGTGGTCGAGATCGACCCTGA

Protein sequence

MSMRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEELITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEIVTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGGRDRP
Homology
BLAST of Carg09387 vs. NCBI nr
Match: KAG7029455.1 (hypothetical protein SDJN02_07794, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 315.1 bits (806), Expect = 3.5e-82
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0

Query: 1   MSMRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60
           MSMRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL
Sbjct: 1   MSMRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60

Query: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120
           ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI
Sbjct: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120

Query: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGGRDRP 159
           VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGGRDRP
Sbjct: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGGRDRP 158

BLAST of Carg09387 vs. NCBI nr
Match: XP_023545212.1 (uncharacterized protein LOC111804691 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 305.1 bits (780), Expect = 3.6e-79
Identity = 156/158 (98.73%), Postives = 156/158 (98.73%), Query Frame = 0

Query: 1   MSMRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60
           MSMRLVEKCVKLSTHKNFLACNKIGY PRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL
Sbjct: 1   MSMRLVEKCVKLSTHKNFLACNKIGYRPRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60

Query: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120
           ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI
Sbjct: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120

Query: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGGRDRP 159
           VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQS GRDRP
Sbjct: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQS-GRDRP 157

BLAST of Carg09387 vs. NCBI nr
Match: XP_022961619.1 (uncharacterized protein LOC111462339 [Cucurbita moschata] >XP_022961620.1 uncharacterized protein LOC111462339 [Cucurbita moschata] >KAG6598519.1 hypothetical protein SDJN03_08297, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 304.3 bits (778), Expect = 6.1e-79
Identity = 154/158 (97.47%), Postives = 156/158 (98.73%), Query Frame = 0

Query: 1   MSMRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60
           MSMRLVEKCVKLSTHKNFLACNK+G+HPR LHNGPDTIDELLDRHVVKKEKSFDDDEEEL
Sbjct: 1   MSMRLVEKCVKLSTHKNFLACNKVGFHPRLLHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60

Query: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120
           ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI
Sbjct: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120

Query: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGGRDRP 159
           VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQS GRDRP
Sbjct: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQS-GRDRP 157

BLAST of Carg09387 vs. NCBI nr
Match: XP_022997345.1 (uncharacterized protein LOC111492289 [Cucurbita maxima] >XP_022997346.1 uncharacterized protein LOC111492289 [Cucurbita maxima] >XP_022997347.1 uncharacterized protein LOC111492289 [Cucurbita maxima])

HSP 1 Score: 303.1 bits (775), Expect = 1.4e-78
Identity = 154/158 (97.47%), Postives = 155/158 (98.10%), Query Frame = 0

Query: 1   MSMRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60
           MSMRLVEKCVKLSTHKNFLACNK GYHPRALHNGPDTIDELLD HVVKKEKSFDDDEEEL
Sbjct: 1   MSMRLVEKCVKLSTHKNFLACNKFGYHPRALHNGPDTIDELLDIHVVKKEKSFDDDEEEL 60

Query: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120
           ITQRRLTSTRRE+LSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI
Sbjct: 61  ITQRRLTSTRRESLSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120

Query: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGGRDRP 159
           VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGG DRP
Sbjct: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGG-DRP 157

BLAST of Carg09387 vs. NCBI nr
Match: XP_038884246.1 (uncharacterized protein LOC120075144 [Benincasa hispida])

HSP 1 Score: 275.4 bits (703), Expect = 3.1e-70
Identity = 135/151 (89.40%), Postives = 144/151 (95.36%), Query Frame = 0

Query: 3   MRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEELIT 62
           MRLV KC+ LS HKNFL CNKIG+HPR LHNGPDT+ ELLDRHVVKKEKSFDD+E+ELIT
Sbjct: 1   MRLVGKCLNLSIHKNFLTCNKIGFHPRVLHNGPDTVAELLDRHVVKKEKSFDDEEDELIT 60

Query: 63  QRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEIVT 122
           QRRLT+TRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDAR+E+DPEIVT
Sbjct: 61  QRRLTTTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARYESDPEIVT 120

Query: 123 RLLIGGRDAVQSALDKLAEKQRQEIDKKQSG 154
           RLLIGGRDAVQSALDKLAEKQ QEIDKKQ+G
Sbjct: 121 RLLIGGRDAVQSALDKLAEKQMQEIDKKQNG 151

BLAST of Carg09387 vs. ExPASy TrEMBL
Match: A0A6J1HEL0 (uncharacterized protein LOC111462339 OS=Cucurbita moschata OX=3662 GN=LOC111462339 PE=4 SV=1)

HSP 1 Score: 304.3 bits (778), Expect = 3.0e-79
Identity = 154/158 (97.47%), Postives = 156/158 (98.73%), Query Frame = 0

Query: 1   MSMRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60
           MSMRLVEKCVKLSTHKNFLACNK+G+HPR LHNGPDTIDELLDRHVVKKEKSFDDDEEEL
Sbjct: 1   MSMRLVEKCVKLSTHKNFLACNKVGFHPRLLHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60

Query: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120
           ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI
Sbjct: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120

Query: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGGRDRP 159
           VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQS GRDRP
Sbjct: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQS-GRDRP 157

BLAST of Carg09387 vs. ExPASy TrEMBL
Match: A0A6J1K786 (uncharacterized protein LOC111492289 OS=Cucurbita maxima OX=3661 GN=LOC111492289 PE=4 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.6e-79
Identity = 154/158 (97.47%), Postives = 155/158 (98.10%), Query Frame = 0

Query: 1   MSMRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60
           MSMRLVEKCVKLSTHKNFLACNK GYHPRALHNGPDTIDELLD HVVKKEKSFDDDEEEL
Sbjct: 1   MSMRLVEKCVKLSTHKNFLACNKFGYHPRALHNGPDTIDELLDIHVVKKEKSFDDDEEEL 60

Query: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120
           ITQRRLTSTRRE+LSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI
Sbjct: 61  ITQRRLTSTRRESLSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120

Query: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGGRDRP 159
           VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGG DRP
Sbjct: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGG-DRP 157

BLAST of Carg09387 vs. ExPASy TrEMBL
Match: A0A6J1CRY1 (uncharacterized protein LOC111014019 OS=Momordica charantia OX=3673 GN=LOC111014019 PE=4 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 7.4e-70
Identity = 132/154 (85.71%), Postives = 147/154 (95.45%), Query Frame = 0

Query: 1   MSMRLVEKCVKLSTHKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEEL 60
           M++RLV KC+ LS HK+FLACNKIG+HPR LHNGPDTIDELLDRHVVKKEKSF+D+++EL
Sbjct: 9   MTIRLVGKCLNLSIHKHFLACNKIGFHPRVLHNGPDTIDELLDRHVVKKEKSFNDEDDEL 68

Query: 61  ITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPEI 120
           ITQR LTSTRREALSLYRDI+RATRFFMWPDSRGVLWRDVLR+NARKEFE+AR+E+DPE+
Sbjct: 69  ITQRHLTSTRREALSLYRDIIRATRFFMWPDSRGVLWRDVLRENARKEFEEARYESDPEV 128

Query: 121 VTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSGG 155
           VTRLLIGGRDAVQSALDKLAEKQ QEIDKKQ GG
Sbjct: 129 VTRLLIGGRDAVQSALDKLAEKQMQEIDKKQHGG 162

BLAST of Carg09387 vs. ExPASy TrEMBL
Match: A0A5A7VCD6 (Complex 1 LYR protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001620 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.8e-67
Identity = 133/154 (86.36%), Postives = 145/154 (94.16%), Query Frame = 0

Query: 1   MSMRLVEKCVKLST-HKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEE 60
           M+MRLV K + LS+  KNFL C+KIG+HPR LHNGPDT++ELLDRHVVKKEKS DDD ++
Sbjct: 1   MTMRLVGKYLNLSSIDKNFLNCSKIGFHPRVLHNGPDTVEELLDRHVVKKEKSLDDDADK 60

Query: 61  LITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPE 120
           LITQRRLTSTRREALSLYRDILRATRFFMWPDS+GVLWRDVLRQNARKEFEDARFE+DPE
Sbjct: 61  LITQRRLTSTRREALSLYRDILRATRFFMWPDSKGVLWRDVLRQNARKEFEDARFESDPE 120

Query: 121 IVTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSG 154
           IVTRLLIGGRDAVQSALDKLAEKQR+EIDKKQSG
Sbjct: 121 IVTRLLIGGRDAVQSALDKLAEKQRKEIDKKQSG 154

BLAST of Carg09387 vs. ExPASy TrEMBL
Match: A0A1S4DWJ1 (uncharacterized protein LOC103488569 OS=Cucumis melo OX=3656 GN=LOC103488569 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.8e-67
Identity = 133/154 (86.36%), Postives = 145/154 (94.16%), Query Frame = 0

Query: 1   MSMRLVEKCVKLST-HKNFLACNKIGYHPRALHNGPDTIDELLDRHVVKKEKSFDDDEEE 60
           M+MRLV K + LS+  KNFL C+KIG+HPR LHNGPDT++ELLDRHVVKKEKS DDD ++
Sbjct: 1   MTMRLVGKYLNLSSIDKNFLNCSKIGFHPRVLHNGPDTVEELLDRHVVKKEKSLDDDADK 60

Query: 61  LITQRRLTSTRREALSLYRDILRATRFFMWPDSRGVLWRDVLRQNARKEFEDARFETDPE 120
           LITQRRLTSTRREALSLYRDILRATRFFMWPDS+GVLWRDVLRQNARKEFEDARFE+DPE
Sbjct: 61  LITQRRLTSTRREALSLYRDILRATRFFMWPDSKGVLWRDVLRQNARKEFEDARFESDPE 120

Query: 121 IVTRLLIGGRDAVQSALDKLAEKQRQEIDKKQSG 154
           IVTRLLIGGRDAVQSALDKLAEKQR+EIDKKQSG
Sbjct: 121 IVTRLLIGGRDAVQSALDKLAEKQRKEIDKKQSG 154

BLAST of Carg09387 vs. TAIR 10
Match: AT1G76060.1 (LYR family of Fe/S cluster biogenesis protein )

HSP 1 Score: 187.2 bits (474), Expect = 1.0e-47
Identity = 95/126 (75.40%), Postives = 108/126 (85.71%), Query Frame = 0

Query: 29  RALHNGPDTIDELLDRHVVKKEKS-FDDDEEELITQRRLTSTRREALSLYRDILRATRFF 88
           R LH GPDT++ELL+RH+ KKEK   D DE E + +RRLTSTRREALSLYRDILRATRFF
Sbjct: 29  RFLHEGPDTVEELLERHLAKKEKPIIDHDEAEFLNRRRLTSTRREALSLYRDILRATRFF 88

Query: 89  MWPDSRGVLWRDVLRQNARKEFEDARFETDPEIVTRLLIGGRDAVQSALDKLAEKQRQEI 148
            W DSRG LWRDVLR+NARKEFE ARFETDPE++TRLLIGG DAV SALDKLAEKQR+ I
Sbjct: 89  TWIDSRGNLWRDVLRENARKEFEAARFETDPEVITRLLIGGSDAVSSALDKLAEKQREMI 148

Query: 149 DKKQSG 154
           +K++ G
Sbjct: 149 EKQRRG 154

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029455.13.5e-82100.00hypothetical protein SDJN02_07794, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023545212.13.6e-7998.73uncharacterized protein LOC111804691 [Cucurbita pepo subsp. pepo][more]
XP_022961619.16.1e-7997.47uncharacterized protein LOC111462339 [Cucurbita moschata] >XP_022961620.1 unchar... [more]
XP_022997345.11.4e-7897.47uncharacterized protein LOC111492289 [Cucurbita maxima] >XP_022997346.1 uncharac... [more]
XP_038884246.13.1e-7089.40uncharacterized protein LOC120075144 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1HEL03.0e-7997.47uncharacterized protein LOC111462339 OS=Cucurbita moschata OX=3662 GN=LOC1114623... [more]
A0A6J1K7866.6e-7997.47uncharacterized protein LOC111492289 OS=Cucurbita maxima OX=3661 GN=LOC111492289... [more]
A0A6J1CRY17.4e-7085.71uncharacterized protein LOC111014019 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A5A7VCD65.8e-6786.36Complex 1 LYR protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82... [more]
A0A1S4DWJ15.8e-6786.36uncharacterized protein LOC103488569 OS=Cucumis melo OX=3656 GN=LOC103488569 PE=... [more]
Match NameE-valueIdentityDescription
AT1G76060.11.0e-4775.40LYR family of Fe/S cluster biogenesis protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008011Complex 1 LYR proteinPFAMPF05347Complex1_LYRcoord: 71..131
e-value: 6.9E-12
score: 45.3
NoneNo IPR availablePANTHERPTHR47484COMPLEX 1 PROTEIN CONTAINING PROTEIN, EXPRESSEDcoord: 6..155
NoneNo IPR availableCDDcd20267Complex1_LYR_LYRM7coord: 73..146
e-value: 1.89082E-17
score: 70.2851

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg09387-RACarg09387-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032981 mitochondrial respiratory chain complex I assembly
cellular_component GO:0005759 mitochondrial matrix