Carg08994 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg08994 vs. NCBI nr
Match: KAG7036344.1 (Beta-carotene isomerase D27, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 503.4 bits (1295), Expect = 1.1e-138
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120
           WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR
Sbjct: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120

Query: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180
           PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE
Sbjct: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180

Query: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240
           QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC
Sbjct: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240

Query: 241 YTL 244
           YTL
Sbjct: 241 YTL 243

BLAST of Carg08994 vs. NCBI nr
Match: XP_022931142.1 (beta-carotene isomerase D27, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 497.3 bits (1279), Expect = 7.6e-137
Identity = 240/243 (98.77%), Postives = 240/243 (98.77%), Query Frame = 0

Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRVFLGNWGNLRGNHKRKLRRKT CCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTTCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120
           WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQRE MIEALQRAFPR
Sbjct: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREAMIEALQRAFPR 120

Query: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180
           PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE
Sbjct: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180

Query: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240
           QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC
Sbjct: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240

Query: 241 YTL 244
           Y L
Sbjct: 241 YKL 243

BLAST of Carg08994 vs. NCBI nr
Match: XP_023534254.1 (beta-carotene isomerase D27, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 492.7 bits (1267), Expect = 1.9e-135
Identity = 237/243 (97.53%), Postives = 238/243 (97.94%), Query Frame = 0

Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRV LGNWGN+RGNHKRKLRRK NCCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVILGNWGNMRGNHKRKLRRKANCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120
           WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQRE MIEALQRAFPR
Sbjct: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREAMIEALQRAFPR 120

Query: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180
           PILSLIR LLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE
Sbjct: 121 PILSLIRALLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180

Query: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240
           QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC
Sbjct: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240

Query: 241 YTL 244
           Y L
Sbjct: 241 YKL 243

BLAST of Carg08994 vs. NCBI nr
Match: XP_022931141.1 (beta-carotene isomerase D27, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 491.1 bits (1263), Expect = 5.5e-135
Identity = 240/248 (96.77%), Postives = 240/248 (96.77%), Query Frame = 0

Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRVFLGNWGNLRGNHKRKLRRKT CCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTTCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATS-----GWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQ 120
           WFDKLAIDHLSEAVQATS     GWRSKKSGYESLVEVATMASRNLNQIKQRE MIEALQ
Sbjct: 61  WFDKLAIDHLSEAVQATSGHMFAGWRSKKSGYESLVEVATMASRNLNQIKQREAMIEALQ 120

Query: 121 RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180
           RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK
Sbjct: 121 RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180

Query: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240
           CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA
Sbjct: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240

Query: 241 LNQPCYTL 244
           LNQPCY L
Sbjct: 241 LNQPCYKL 248

BLAST of Carg08994 vs. NCBI nr
Match: XP_023534253.1 (beta-carotene isomerase D27, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 486.5 bits (1251), Expect = 1.3e-133
Identity = 237/248 (95.56%), Postives = 238/248 (95.97%), Query Frame = 0

Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRV LGNWGN+RGNHKRKLRRK NCCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVILGNWGNMRGNHKRKLRRKANCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATS-----GWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQ 120
           WFDKLAIDHLSEAVQATS     GWRSKKSGYESLVEVATMASRNLNQIKQRE MIEALQ
Sbjct: 61  WFDKLAIDHLSEAVQATSGHIFAGWRSKKSGYESLVEVATMASRNLNQIKQREAMIEALQ 120

Query: 121 RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180
           RAFPRPILSLIR LLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK
Sbjct: 121 RAFPRPILSLIRALLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180

Query: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240
           CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA
Sbjct: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240

Query: 241 LNQPCYTL 244
           LNQPCY L
Sbjct: 241 LNQPCYKL 248

BLAST of Carg08994 vs. ExPASy Swiss-Prot
Match: R4HZ96 (Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 6.8e-80
Identity = 147/246 (59.76%), Postives = 182/246 (73.98%), Query Frame = 0

Query: 1   MDVKLIQERRVF---LGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVY 60
           MD K+I         L  W  LR      L+ K    V VL    +    E  R  N VY
Sbjct: 1   MDSKMIAHNMSLTPTLAQWKKLR------LKPKHTFVVGVLARPTDDISEETLRKTN-VY 60

Query: 61  TDNWFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRA 120
            DNWFDKLAI+HLS++VQA +G  + KSG++SLVE AT+AS+  N  +Q+ ++++AL RA
Sbjct: 61  KDNWFDKLAINHLSKSVQAATGISNNKSGFDSLVEAATVASQKFNTTQQQGIILDALDRA 120

Query: 121 FPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCR 180
           FP+PILS+IR ++P SK AREYFA FTT+FFAWL+GP EV+ESE+NGRRE+N+VHI KCR
Sbjct: 121 FPKPILSVIRRVMPPSKLAREYFAVFTTIFFAWLLGPSEVRESEINGRREKNIVHIKKCR 180

Query: 181 FLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALN 240
           FLE+TNCVGMCINLCK P+Q FIKDS GMPV+M+PNFDDMSCEM FG++PPAS +DPAL 
Sbjct: 181 FLEETNCVGMCINLCKMPSQLFIKDSFGMPVNMVPNFDDMSCEMIFGQEPPASTDDPALK 239

Query: 241 QPCYTL 244
           QPCY L
Sbjct: 241 QPCYKL 239

BLAST of Carg08994 vs. ExPASy Swiss-Prot
Match: C7AU21 (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 8.1e-65
Identity = 116/187 (62.03%), Postives = 146/187 (78.07%), Query Frame = 0

Query: 55  TVYTDNWFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEAL 114
           TVY D+WFDKLAI +LS  +Q  SG +++K GYESL++ A   SR  +  KQ E++ +AL
Sbjct: 78  TVYRDSWFDKLAIGYLSRNLQEASGLKNEKDGYESLIDAALAISRIFSLDKQSEIVTQAL 137

Query: 115 QRAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIH 174
           +RA P  IL++I+ ++P S+F+REYFAAFTT+FF WLVGPCEV ESE+ GR+E+NVV+I 
Sbjct: 138 ERALPSYILTMIKVMMPPSRFSREYFAAFTTIFFPWLVGPCEVMESEVEGRKEKNVVYIP 197

Query: 175 KCRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDP 234
           KCRFLE TNCVGMC NLCK P Q FI+DSLGM V M PNF+DMSCEM FG+ PP   +DP
Sbjct: 198 KCRFLESTNCVGMCTNLCKIPCQKFIQDSLGMKVYMSPNFEDMSCEMIFGQQPPE--DDP 257

Query: 235 ALNQPCY 242
           AL QPC+
Sbjct: 258 ALKQPCF 262

BLAST of Carg08994 vs. ExPASy Swiss-Prot
Match: Q7XA78 (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 3.4e-55
Identity = 115/251 (45.82%), Postives = 163/251 (64.94%), Query Frame = 0

Query: 1   MDVKL-IQERRVF-LGNWGN--LRGNHKRKLRRKTNCCVAVLTG----AAERGGNEGTRN 60
           M+ KL + + ++F    W N    G  +R     T C   V +G    A E    E +  
Sbjct: 1   MNTKLSLSQTKIFTFTTWFNDTRSGLDRRSSISPTLCSKPVYSGKLKAAKETARIETSNT 60

Query: 61  LNTVYTDNWFDKLAIDHLSEAVQATSG--WRSKKSGYESLVEVATMASRNLNQIKQREVM 120
            N    D++F K+AI++LS+ +Q  +G    SK + Y+ LV+ AT  SRN +  +Q E +
Sbjct: 61  KNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVSRNFDTKQQHEFV 120

Query: 121 IEALQRAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNV 180
           + +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP EV+E+E+NGR+E++V
Sbjct: 121 LSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPSEVRETEVNGRKEKSV 180

Query: 181 VHIHKCRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPAS 240
           V+I KCRFLEQ+NCVGMC ++CK P+Q FIK+SLGMP+ M P+F+D+SC+M FG +PP  
Sbjct: 181 VYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGREPPEI 240

Query: 241 IEDPALNQPCY 242
            +DPA+ QPC+
Sbjct: 241 EDDPAMKQPCF 251

BLAST of Carg08994 vs. ExPASy TrEMBL
Match: A0A6J1ESQ1 (beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111437410 PE=4 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 3.7e-137
Identity = 240/243 (98.77%), Postives = 240/243 (98.77%), Query Frame = 0

Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRVFLGNWGNLRGNHKRKLRRKT CCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTTCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120
           WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQRE MIEALQRAFPR
Sbjct: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREAMIEALQRAFPR 120

Query: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180
           PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE
Sbjct: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180

Query: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240
           QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC
Sbjct: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240

Query: 241 YTL 244
           Y L
Sbjct: 241 YKL 243

BLAST of Carg08994 vs. ExPASy TrEMBL
Match: A0A6J1ESU9 (beta-carotene isomerase D27, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111437410 PE=4 SV=1)

HSP 1 Score: 491.1 bits (1263), Expect = 2.6e-135
Identity = 240/248 (96.77%), Postives = 240/248 (96.77%), Query Frame = 0

Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRVFLGNWGNLRGNHKRKLRRKT CCVAVLTGAAERGGNEGTRNLNTVYTDN
Sbjct: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTTCCVAVLTGAAERGGNEGTRNLNTVYTDN 60

Query: 61  WFDKLAIDHLSEAVQATS-----GWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQ 120
           WFDKLAIDHLSEAVQATS     GWRSKKSGYESLVEVATMASRNLNQIKQRE MIEALQ
Sbjct: 61  WFDKLAIDHLSEAVQATSGHMFAGWRSKKSGYESLVEVATMASRNLNQIKQREAMIEALQ 120

Query: 121 RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180
           RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK
Sbjct: 121 RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180

Query: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240
           CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA
Sbjct: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240

Query: 241 LNQPCYTL 244
           LNQPCY L
Sbjct: 241 LNQPCYKL 248

BLAST of Carg08994 vs. ExPASy TrEMBL
Match: A0A6J1K233 (beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111491020 PE=4 SV=1)

HSP 1 Score: 481.1 bits (1237), Expect = 2.7e-132
Identity = 232/243 (95.47%), Postives = 234/243 (96.30%), Query Frame = 0

Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRV LGNWGNLRGNHKRKLRR  NCCVAVLTGAAERGGNE TRNLN VY DN
Sbjct: 1   MDVKLIQERRVILGNWGNLRGNHKRKLRRNANCCVAVLTGAAERGGNEETRNLNAVYRDN 60

Query: 61  WFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFPR 120
           WFDKLAIDHLSEAVQATSGWRS+KSGYESLVEVATMASRNLNQIKQRE MIEALQRAFPR
Sbjct: 61  WFDKLAIDHLSEAVQATSGWRSEKSGYESLVEVATMASRNLNQIKQREAMIEALQRAFPR 120

Query: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180
           PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE
Sbjct: 121 PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLE 180

Query: 181 QTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 240
           QTNCVGMCINLCKFPTQ+FIKDSLGMPVSM PNFDDMSCEMKFGEDPPASIEDPALNQPC
Sbjct: 181 QTNCVGMCINLCKFPTQEFIKDSLGMPVSMTPNFDDMSCEMKFGEDPPASIEDPALNQPC 240

Query: 241 YTL 244
           Y L
Sbjct: 241 YKL 243

BLAST of Carg08994 vs. ExPASy TrEMBL
Match: A0A6J1K5Z4 (beta-carotene isomerase D27, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491020 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.0e-130
Identity = 232/248 (93.55%), Postives = 234/248 (94.35%), Query Frame = 0

Query: 1   MDVKLIQERRVFLGNWGNLRGNHKRKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDN 60
           MDVKLIQERRV LGNWGNLRGNHKRKLRR  NCCVAVLTGAAERGGNE TRNLN VY DN
Sbjct: 1   MDVKLIQERRVILGNWGNLRGNHKRKLRRNANCCVAVLTGAAERGGNEETRNLNAVYRDN 60

Query: 61  WFDKLAIDHLSEAVQATS-----GWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQ 120
           WFDKLAIDHLSEAVQATS     GWRS+KSGYESLVEVATMASRNLNQIKQRE MIEALQ
Sbjct: 61  WFDKLAIDHLSEAVQATSAHIFAGWRSEKSGYESLVEVATMASRNLNQIKQREAMIEALQ 120

Query: 121 RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180
           RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK
Sbjct: 121 RAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHK 180

Query: 181 CRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPA 240
           CRFLEQTNCVGMCINLCKFPTQ+FIKDSLGMPVSM PNFDDMSCEMKFGEDPPASIEDPA
Sbjct: 181 CRFLEQTNCVGMCINLCKFPTQEFIKDSLGMPVSMTPNFDDMSCEMKFGEDPPASIEDPA 240

Query: 241 LNQPCYTL 244
           LNQPCY L
Sbjct: 241 LNQPCYKL 248

BLAST of Carg08994 vs. ExPASy TrEMBL
Match: A0A6J1HWX8 (beta-carotene isomerase D27, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466998 PE=4 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 8.9e-107
Identity = 191/244 (78.28%), Postives = 217/244 (88.93%), Query Frame = 0

Query: 1   MDVKLIQERRVFLGNWGNLRGNHK-RKLRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTD 60
           M+V++ +ERRV LG+  N+R ++K +K+RRK+  CVAVLT AAE G  E TR  NTVYTD
Sbjct: 1   MEVRVGEERRVILGSGTNVRRSNKTKKMRRKSIACVAVLTRAAESGATEETRKPNTVYTD 60

Query: 61  NWFDKLAIDHLSEAVQATSGWRSKKSGYESLVEVATMASRNLNQIKQREVMIEALQRAFP 120
           NWF+K+AI H+S+AVQATSGWRSKKSGYESLVEVATMASRN NQIKQREV+I+AL RAFP
Sbjct: 61  NWFEKIAIHHVSQAVQATSGWRSKKSGYESLVEVATMASRNYNQIKQREVIIQALGRAFP 120

Query: 121 RPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFL 180
            PILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKES+  G++E+NVV IHKCRFL
Sbjct: 121 APILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESDFKGKKEKNVVQIHKCRFL 180

Query: 181 EQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQP 240
           EQTNC GMCINLCKFP QDFIKDSLGMPVSM+PNFDDMSCEM FG+DPPA+++DPAL QP
Sbjct: 181 EQTNCAGMCINLCKFPCQDFIKDSLGMPVSMVPNFDDMSCEMIFGKDPPAAVDDPALKQP 240

Query: 241 CYTL 244
           CY L
Sbjct: 241 CYKL 244

BLAST of Carg08994 vs. TAIR 10
Match: AT1G03055.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 216.5 bits (550), Expect = 2.4e-56
Identity = 115/251 (45.82%), Postives = 163/251 (64.94%), Query Frame = 0

Query: 1   MDVKL-IQERRVF-LGNWGN--LRGNHKRKLRRKTNCCVAVLTG----AAERGGNEGTRN 60
           M+ KL + + ++F    W N    G  +R     T C   V +G    A E    E +  
Sbjct: 1   MNTKLSLSQTKIFTFTTWFNDTRSGLDRRSSISPTLCSKPVYSGKLKAAKETARIETSNT 60

Query: 61  LNTVYTDNWFDKLAIDHLSEAVQATSG--WRSKKSGYESLVEVATMASRNLNQIKQREVM 120
            N    D++F K+AI++LS+ +Q  +G    SK + Y+ LV+ AT  SRN +  +Q E +
Sbjct: 61  KNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVSRNFDTKQQHEFV 120

Query: 121 IEALQRAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNV 180
           + +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP EV+E+E+NGR+E++V
Sbjct: 121 LSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPSEVRETEVNGRKEKSV 180

Query: 181 VHIHKCRFLEQTNCVGMCINLCKFPTQDFIKDSLGMPVSMIPNFDDMSCEMKFGEDPPAS 240
           V+I KCRFLEQ+NCVGMC ++CK P+Q FIK+SLGMP+ M P+F+D+SC+M FG +PP  
Sbjct: 181 VYIEKCRFLEQSNCVGMCTHICKIPSQIFIKNSLGMPIYMEPDFNDLSCKMMFGREPPEI 240

Query: 241 IEDPALNQPCY 242
            +DPA+ QPC+
Sbjct: 241 EDDPAMKQPCF 251

BLAST of Carg08994 vs. TAIR 10
Match: AT1G64680.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 166.0 bits (419), Expect = 3.7e-41
Identity = 87/221 (39.37%), Postives = 123/221 (55.66%), Query Frame = 0

Query: 28  RRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDNWFDKLAIDHLSEAVQATSGWRSKKS-- 87
           RR T C      G AE  G      L T Y D   +++ +   +  +      + K +  
Sbjct: 16  RRSTRC------GIAEPSGEPAPMGLKTRYEDGLVERVFMGLFARKMDKFGSKKKKDTKE 75

Query: 88  ------GYESLVEVATMASRNLNQIKQREVMIEALQRAFPRPILSLIRTLLPQSKFAREY 147
                  YES VEV+    +  ++++Q+E + E L    P       R L P +K+A E+
Sbjct: 76  KGFWEYDYESFVEVSKRVMQGRSRVQQQEAVREVLLSMLPPGAPEQFRKLFPPTKWAAEF 135

Query: 148 FAAFTTVFFAWLVGPCEVKESELNGRRERNVVHIHKCRFLEQTNCVGMCINLCKFPTQDF 207
            AA T  FF WLVGP +V E E+NG ++R+ V I KCR+LE + CVGMC+N+CK PTQDF
Sbjct: 136 NAALTVPFFHWLVGPSQVIEVEVNGVKQRSGVRIKKCRYLENSGCVGMCVNMCKIPTQDF 195

Query: 208 IKDSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPC 241
             +  G+P++M PN++DMSCEM +G+ PPA  ED A  QPC
Sbjct: 196 FTNEFGLPLTMNPNYEDMSCEMIYGQAPPAFEEDVATKQPC 230

BLAST of Carg08994 vs. TAIR 10
Match: AT1G03055.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 123.6 bits (309), Expect = 2.1e-28
Identity = 76/187 (40.64%), Postives = 110/187 (58.82%), Query Frame = 0

Query: 1   MDVKL-IQERRVF-LGNWGN--LRGNHKRKLRRKTNCCVAVLTG----AAERGGNEGTRN 60
           M+ KL + + ++F    W N    G  +R     T C   V +G    A E    E +  
Sbjct: 1   MNTKLSLSQTKIFTFTTWFNDTRSGLDRRSSISPTLCSKPVYSGKLKAAKETARIETSNT 60

Query: 61  LNTVYTDNWFDKLAIDHLSEAVQATSG--WRSKKSGYESLVEVATMASRNLNQIKQREVM 120
            N    D++F K+AI++LS+ +Q  +G    SK + Y+ LV+ AT  SRN +  +Q E +
Sbjct: 61  KNASIEDSFFSKIAINYLSKNLQDAAGISSSSKSTDYDRLVDTATRVSRNFDTKQQHEFV 120

Query: 121 IEALQRAFPRPILSLIRTLLPQSKFAREYFAAFTTVFFAWLVGPCEVKESELNGRRERNV 178
           + +L RA P  I SLI+   P SK +RE FA FTT+ FAWLVGP EV+E+E+NGR+E++V
Sbjct: 121 LSSLDRALPTVISSLIKMAFPPSKVSRELFALFTTISFAWLVGPSEVRETEVNGRKEKSV 180

BLAST of Carg08994 vs. TAIR 10
Match: AT4G01995.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 119.4 bits (298), Expect = 4.0e-27
Identity = 74/220 (33.64%), Postives = 112/220 (50.91%), Query Frame = 0

Query: 27  LRRKTNCCVAVLTGAAERGGNEGTRNLNTVYTDNWFDKLAIDHLSEAVQATSGWRSKKSG 86
           L R+  C +   + ++E   +EG   L   Y     D   +      +    G  S+K G
Sbjct: 27  LTRRLRCRI---SNSSEVKSDEGAPKLE--YKPGPLDDFFMQSFRNKLVEEVGSDSEKPG 86

Query: 87  YESLVE-VATMASRNLNQIKQREVMIEALQRAFPRPILSLIRTL---LPQSKFAREYFAA 146
           Y  L+E V  +  +   + +  +  +  L+  FP  IL L + L   + Q K A    A 
Sbjct: 87  YVGLIELVKLLLLKGRTRSETSDAAVRILKSLFPPLILELYKLLIAPIAQGKLAALMVAR 146

Query: 147 FTTVFFAWLVGPCEVKESEL-NGRRERNVVHIHKCRFLEQTNCVGMCINLCKFPTQDFIK 206
            T +   WL+GP +V   +L NG    + V + KC++LE++ CVG+CIN CK PTQ F K
Sbjct: 147 VTVLTCQWLMGPSKVNIIDLPNGESWDSGVFVEKCQYLEESKCVGVCINTCKLPTQTFFK 206

Query: 207 DSLGMPVSMIPNFDDMSCEMKFGEDPPASIEDPALNQPCY 242
           D +G+P+ M PNF D SC+ KFG  PP   +D  +N+PC+
Sbjct: 207 DYMGVPLVMEPNFKDYSCQFKFGVAPPE--DDGNVNEPCF 239