Carg08903 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08903
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Cytochrome b5
Location	Carg_Chr02: 9379656 .. 9381764 (+)
RNA-Seq Expression	Carg08903
Synteny	Carg08903

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TACCCATCGCTCAATAGGGAATAAAATCAATGTTAAGATTCATGATCACAACAACCCCACTTTCCACTTCCCTTCAAAACCTCAAGAAACAGAGCCCTTTTGGTGCAAGAGGAAAACAGGAAAGAATTGTCTGCCACTGCCAGATTACACAAACTTCTCTTATCTCTATCTCTATCTCTCTCCCTCTTCCAAGCTGCGACGATGGTTGGTAGCACCGTGTTCACTTTGGCTGAGGTTGCCTCCCACGACAACCGCAACGACTGCTGGTTGATCATTGAGGACAAGGTATTTTATCATTTGTCTCTGTGGCTCAATGTGGTTTCAATTTCTTTACATGGCTTTCATTTTCTGTTTGATCTTGAAATTTATGCATATAAATACTTCTTGTTTTGTGTTTACTTTTTTCGCAGACTAAAAAAATAGTTCTTGTTTTTGGAATTTGGTTAAGGATGAAATCTATAGAAAATATGATTTGACGTTGCTGAAGTGTTAAAGATTCATTGAGTCTATTTGCTTTGACTGATTGAGTTTCATGGATGGAACTAGTGCTCTTCTGTGTATTGTTTGATCTGAATTGGGCTTACGAATCGAAGTTTGGTGTATGTAAACGATTTGTCACTGAATTTGTAATTTATTTATCATGTAGGACATGTACTTTTCTCTACTAGATCCGATTCTTTTATTTTTATTGTTTGGATAGGAGTACCCGTAATAGCTTTAGCCGACCGCTAGCATATATTGTCATCTTTGAGCTTTTCCTTCAAGATTTTTAAAACGTGCCTACTAGGGAGAGGTTTCCATACCCTTATAAACAATTTTTCATCGACCGCTAGCATATATTATCATCTTTGAGCTTTTCCTTCAAGATTTTTAAAACGTGCCTACTAGGGAGAGGTTTCCATACCCTTATAAAGAATTTTTCATCGACCGCTAGCATATATTATCATCTTTGAGCTTTTCCTTCAAGATTTTTAAAACGTGCCTACTAGGGAGAGGTTTTCATACCCTTATAAAGAATTTTTCATTTTCCTCCTCAACCGACGTGAGATCTCATAATTCATTTCCCTTCGAGGTGCAGCGTGTTCACTGGCACTCGTTTCCTTCTCCGAAGGATGTGGGACCGTCCAATCCACCCCCTTTTGCGGCCTAGCGTCCTTGTTGGCACACTGCCTCGTGTCCATTCCCTTTGGGGCTCAACCTCCTTGCTGGCATATTGTCCGGTGCTGGCTCTAATACCATTTGTAACAGTCCAAGTTCATCGTTAGCGTATATTGTCGTTTTTTAGTTTCCCCCTAGAACTTTTTAAAAGAGAGGTTTCTACCCCCTTATAAAAAATGTTTCGTTCTCGTCTCCAATCGATGTGCAATCTCGTTCCCTCTCTCTCTCTCAATTGTTTTTCAATTTTGTGTGTTATTGGTCCAACAGGTGTACGATGTAACAAAGTTCCTTGAAGACCATCCTGGTGGTGATGAAGTCTTGTTGTCAGCCATTGGTAGGGAAAAACAGGCAACGAAAATTTTTCATTGAAATATATTCTTAATAAGCTTTGTCAAATGAACTGATCATAATGTTTGAGATACCAATGTTGGTTCTTCATATCATCTCATCTACAGGTAAGGATGCAACCAACGATTTTTTCGATGTCGGGCACAGTAGCGCTGCTCGAGCGATGATGGAAGAATTTTACGTCGGCGACATCGATAGCTCAACTATCCCTACAAAAAGGAAGTACACTCCTCCAAAGCAGCCCCTATACAACCAAGACAAGACACCAGAGTTCATTATCAAGGTCCTCCAGTTTCTAGCTCCCATGGTAATTTTGGCATTGGCTTTTGGCATCCATCTCTATACCAAAACAACATGAACACTGAAACTATATCCATTTTTTTTGAAAAAATTGGTATATTTTGTTCCAATGAGTGTACTATGCTTGAAACTTGTTATATGATGCCGCTGAAAAATGTTTCAACTTCCTCTGTGATATAATGTTTACTTACCAAAAACATTATCAATAAAGAGTAGTTCTTGGCAATGTTATTCATTTACGAATACAAGTAGACTGAATTATTCTCCAAAGAGAGGAGAAAAGAACTTGCATTTGTTCAACAA

mRNA sequence

TACCCATCGCTCAATAGGGAATAAAATCAATGTTAAGATTCATGATCACAACAACCCCACTTTCCACTTCCCTTCAAAACCTCAAGAAACAGAGCCCTTTTGGTGCAAGAGGAAAACAGGAAAGAATTGTCTGCCACTGCCAGATTACACAAACTTCTCTTATCTCTATCTCTATCTCTCTCCCTCTTCCAAGCTGCGACGATGGTTGGTAGCACCGTGTTCACTTTGGCTGAGGTTGCCTCCCACGACAACCGCAACGACTGCTGGTTGATCATTGAGGACAAGGTGTACGATGTAACAAAGTTCCTTGAAGACCATCCTGGTGGTGATGAAGTCTTGTTGTCAGCCATTGGTAAGGATGCAACCAACGATTTTTTCGATGTCGGGCACAGTAGCGCTGCTCGAGCGATGATGGAAGAATTTTACGTCGGCGACATCGATAGCTCAACTATCCCTACAAAAAGGAAGTACACTCCTCCAAAGCAGCCCCTATACAACCAAGACAAGACACCAGAGTTCATTATCAAGGTCCTCCAGTTTCTAGCTCCCATGGTAATTTTGGCATTGGCTTTTGGCATCCATCTCTATACCAAAACAACATGAACACTGAAACTATATCCATTTTTTTTGAAAAAATTGGTATATTTTGTTCCAATGAGTGTACTATGCTTGAAACTTGTTATATGATGCCGCTGAAAAATGTTTCAACTTCCTCTGTGATATAATGTTTACTTACCAAAAACATTATCAATAAAGAGTAGTTCTTGGCAATGTTATTCATTTACGAATACAAGTAGACTGAATTATTCTCCAAAGAGAGGAGAAAAGAACTTGCATTTGTTCAACAA

Coding sequence (CDS)

ATGGTTGGTAGCACCGTGTTCACTTTGGCTGAGGTTGCCTCCCACGACAACCGCAACGACTGCTGGTTGATCATTGAGGACAAGGTGTACGATGTAACAAAGTTCCTTGAAGACCATCCTGGTGGTGATGAAGTCTTGTTGTCAGCCATTGGTAAGGATGCAACCAACGATTTTTTCGATGTCGGGCACAGTAGCGCTGCTCGAGCGATGATGGAAGAATTTTACGTCGGCGACATCGATAGCTCAACTATCCCTACAAAAAGGAAGTACACTCCTCCAAAGCAGCCCCTATACAACCAAGACAAGACACCAGAGTTCATTATCAAGGTCCTCCAGTTTCTAGCTCCCATGGTAATTTTGGCATTGGCTTTTGGCATCCATCTCTATACCAAAACAACATGA

Protein sequence

MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFDVGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVILALAFGIHLYTKTT

Homology

[Close]

BLAST of Carg08903 vs. NCBI nr
Match: KAG6606316.1 (hypothetical protein SDJN03_03633, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036255.1 hypothetical protein SDJN02_03058 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 272.7 bits (696), Expect = 1.7e-69
Identity = 133/133 (100.00%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD
Sbjct: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL
Sbjct: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120

Query: 121 ALAFGIHLYTKTT 134
           ALAFGIHLYTKTT
Sbjct: 121 ALAFGIHLYTKTT 133

[Close]

BLAST of Carg08903 vs. NCBI nr
Match: XP_023534065.1 (cytochrome b5, seed isoform-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 271.6 bits (693), Expect = 3.7e-69
Identity = 132/133 (99.25%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD
Sbjct: 56  MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 115

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAP+VIL
Sbjct: 116 VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 175

Query: 121 ALAFGIHLYTKTT 134
           ALAFGIHLYTKTT
Sbjct: 176 ALAFGIHLYTKTT 188

[Close]

BLAST of Carg08903 vs. NCBI nr
Match: XP_022931194.1 (cytochrome b5, seed isoform-like [Cucurbita moschata])

HSP 1 Score: 270.8 bits (691), Expect = 6.3e-69
Identity = 130/133 (97.74%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD
Sbjct: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           VGHSSAARAMMEEFY+GDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAP+VIL
Sbjct: 61  VGHSSAARAMMEEFYIGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 ALAFGIHLYTKTT 134
           ALAFG+HLYTKTT
Sbjct: 121 ALAFGVHLYTKTT 133

[Close]

BLAST of Carg08903 vs. NCBI nr
Match: XP_022995981.1 (cytochrome b5, seed isoform-like [Cucurbita maxima])

HSP 1 Score: 268.9 bits (686), Expect = 2.4e-68
Identity = 130/133 (97.74%), Postives = 132/133 (99.25%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           MVGST+FTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD
Sbjct: 1   MVGSTLFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           VGHSS ARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAP+VIL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 ALAFGIHLYTKTT 134
           ALAFGIHLYTKTT
Sbjct: 121 ALAFGIHLYTKTT 133

[Close]

BLAST of Carg08903 vs. NCBI nr
Match: XP_038889018.1 (cytochrome b5, seed isoform isoform X3 [Benincasa hispida])

HSP 1 Score: 257.3 bits (656), Expect = 7.3e-65
Identity = 123/133 (92.48%), Postives = 127/133 (95.49%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           M G TVF+LAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLS +GKDATNDFFD
Sbjct: 1   MGGRTVFSLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           VGHSSAARAMMEEFYVGDIDSSTIP KR YTPPKQPLYNQDKTPEFIIKVLQFLAP++IL
Sbjct: 61  VGHSSAARAMMEEFYVGDIDSSTIPAKRTYTPPKQPLYNQDKTPEFIIKVLQFLAPLIIL 120

Query: 121 ALAFGIHLYTKTT 134
            LAFGIHLYTKTT
Sbjct: 121 GLAFGIHLYTKTT 133

[Close]

BLAST of Carg08903 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 4.6e-54
Identity = 98/128 (76.56%), Postives = 114/128 (89.06%), Query Frame = 0


Query: 4   STVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFDVGH 63
           S VFTLAEV++H+N  DCWLII  KVY+VTKFLEDHPGG EVLLSA GKDAT+DF D+GH
Sbjct: 5   SKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFEDIGH 64

Query: 64  SSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVILALA 123
           SS+ARAM++E+YVGDIDSSTIPTK KYTPPKQP YNQDKT EFI+K+LQFL P++IL +A
Sbjct: 65  SSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLIILGVA 124

Query: 124 FGIHLYTK 132
           FG+H YTK
Sbjct: 125 FGVHFYTK 132

[Close]

BLAST of Carg08903 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 3.0e-53
Identity = 98/132 (74.24%), Postives = 114/132 (86.36%), Query Frame = 0


Query: 1   MVGST-VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFF 60
           M G T VFTLAEV+ H+N  DCWL+I  KVYDVTKFL+DHPGGDEVLLSA GKDAT+DF 
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVI 120
           DVGHSS+ARAM++E+YVGDIDS+TIPTK KYTPP QP YNQDKT EF++K+LQFL P++I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LALAFGIHLYTK 132
           L +AFGI  YTK
Sbjct: 121 LGVAFGIRFYTK 132

[Close]

BLAST of Carg08903 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 7.3e-52
Identity = 95/128 (74.22%), Postives = 113/128 (88.28%), Query Frame = 0


Query: 6   VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFDVGHSS 65
           +FTLAEVA H+N  DCWLII  KVYDVTKFLEDHPGGD+VLLSA GKDAT+DF D+GHSS
Sbjct: 4   IFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGHSS 63

Query: 66  AARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVILALAFG 125
           +A+AM++E+YVGDIDSS+IP++ KYTPPKQPLYN DKT EF+IK+LQFL P+VILA A G
Sbjct: 64  SAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQFLVPLVILAGAIG 123

Query: 126 IHLYTKTT 134
           I  YTK++
Sbjct: 124 IRFYTKSS 131

[Close]

BLAST of Carg08903 vs. ExPASy Swiss-Prot
Match: P49097 (Cytochrome b5 OS=Cuscuta reflexa OX=4129 PE=2 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 4.0e-50
Identity = 93/131 (70.99%), Postives = 109/131 (83.21%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           M GS V++LAEV+ H   NDCWL+I  KVYDVTKFL+DHPGG +VLLS+  KDAT+DF D
Sbjct: 1   MGGSKVYSLAEVSEHSQPNDCWLVIGGKVYDVTKFLDDHPGGADVLLSSTAKDATDDFED 60

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           +GHSS+ARAMM+E  VGDIDSSTIPTK  YTPPKQPLYNQDKTP+FIIK+LQFL P++IL
Sbjct: 61  IGHSSSARAMMDEMCVGDIDSSTIPTKTSYTPPKQPLYNQDKTPQFIIKLLQFLVPLIIL 120

Query: 121 ALAFGIHLYTK 132
            +A GI  Y K
Sbjct: 121 GVAVGIRFYKK 131

[Close]

BLAST of Carg08903 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 198.4 bits (503), Expect = 5.2e-50
Identity = 91/127 (71.65%), Postives = 110/127 (86.61%), Query Frame = 0


Query: 6   VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFDVGHSS 65
           V+TL EVA H++++DCWLII  KVY+V+KFLEDHPGGD+VLLS+ GKDAT+DF DVGHS+
Sbjct: 8   VYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGHST 67

Query: 66  AARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVILALAFG 125
            ARAMM+E+YVGDID+STIP + KY PPKQP YNQDKTPEFIIK+LQFL P+ IL LA  
Sbjct: 68  TARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLAVA 127

Query: 126 IHLYTKT 133
           I +YTK+
Sbjct: 128 IRIYTKS 134

[Close]

BLAST of Carg08903 vs. ExPASy TrEMBL
Match: A0A6J1ETM5 (cytochrome b5, seed isoform-like OS=Cucurbita moschata OX=3662 GN=LOC111437450 PE=3 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 3.1e-69
Identity = 130/133 (97.74%), Postives = 133/133 (100.00%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD
Sbjct: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           VGHSSAARAMMEEFY+GDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAP+VIL
Sbjct: 61  VGHSSAARAMMEEFYIGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 ALAFGIHLYTKTT 134
           ALAFG+HLYTKTT
Sbjct: 121 ALAFGVHLYTKTT 133

[Close]

BLAST of Carg08903 vs. ExPASy TrEMBL
Match: A0A6J1K3E7 (cytochrome b5, seed isoform-like OS=Cucurbita maxima OX=3661 GN=LOC111491329 PE=3 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 1.2e-68
Identity = 130/133 (97.74%), Postives = 132/133 (99.25%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           MVGST+FTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD
Sbjct: 1   MVGSTLFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           VGHSS ARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAP+VIL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 ALAFGIHLYTKTT 134
           ALAFGIHLYTKTT
Sbjct: 121 ALAFGIHLYTKTT 133

[Close]

BLAST of Carg08903 vs. ExPASy TrEMBL
Match: A0A5A7UKQ8 (Cytochrome b5, seed isoform-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1278G00070 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.3e-64
Identity = 122/133 (91.73%), Postives = 126/133 (94.74%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           M G +VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLS +GKDATNDFFD
Sbjct: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           VGHSS ARAMMEEFYVGDIDSSTIP KR+YTPPKQPLYNQDKTPEFIIKVLQFLAP+VIL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 ALAFGIHLYTKTT 134
            LAFGIHLY KTT
Sbjct: 121 GLAFGIHLYIKTT 133

[Close]

BLAST of Carg08903 vs. ExPASy TrEMBL
Match: A0A1S3CMW3 (cytochrome b5, seed isoform-like OS=Cucumis melo OX=3656 GN=LOC103502759 PE=3 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.3e-64
Identity = 122/133 (91.73%), Postives = 126/133 (94.74%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           M G +VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLS +GKDATNDFFD
Sbjct: 1   MGGRSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           VGHSS ARAMMEEFYVGDIDSSTIP KR+YTPPKQPLYNQDKTPEFIIKVLQFLAP+VIL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKREYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 ALAFGIHLYTKTT 134
            LAFGIHLY KTT
Sbjct: 121 GLAFGIHLYIKTT 133

[Close]

BLAST of Carg08903 vs. ExPASy TrEMBL
Match: A0A0A0LCD6 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G627730 PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.9e-64
Identity = 122/133 (91.73%), Postives = 125/133 (93.98%), Query Frame = 0


Query: 1   MVGSTVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFD 60
           M G +VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLS +GKDATNDFFD
Sbjct: 1   MGGHSVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSGVGKDATNDFFD 60

Query: 61  VGHSSAARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVIL 120
           VGHSS ARAMMEEFYVGDIDSSTIP KR YTPPKQPLYNQDKTPEFIIKVLQFLAP+VIL
Sbjct: 61  VGHSSTARAMMEEFYVGDIDSSTIPAKRDYTPPKQPLYNQDKTPEFIIKVLQFLAPLVIL 120

Query: 121 ALAFGIHLYTKTT 134
            LAFGIHLY KTT
Sbjct: 121 GLAFGIHLYIKTT 133

[Close]

BLAST of Carg08903 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 193.4 bits (490), Expect = 1.2e-49
Identity = 88/127 (69.29%), Postives = 108/127 (85.04%), Query Frame = 0


Query: 6   VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFDVGHSS 65
           +FTL+EV+ H+  +DCW++I  KVY+VTKFLEDHPGGD+VLLS+ GKDAT+DF DVGHS 
Sbjct: 7   IFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVGHSE 66

Query: 66  AARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVILALAFG 125
           +AR MME++YVG+ID +TIP K KYTPPKQP YNQDKT EFIIK+LQFL P+ IL LA G
Sbjct: 67  SAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAVG 126

Query: 126 IHLYTKT 133
           I +YTK+
Sbjct: 127 IRIYTKS 133

[Close]

BLAST of Carg08903 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 178.7 bits (452), Expect = 3.1e-45
Identity = 80/129 (62.02%), Postives = 107/129 (82.95%), Query Frame = 0


Query: 6   VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFDVGHSS 65
           VFTL+EV+ H +  DCW++I+ KVYDVTKFL+DHPGGDEV+L++ GKDAT+DF DVGHSS
Sbjct: 7   VFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVGHSS 66

Query: 66  AARAMMEEFYVGDIDSSTIPTKRKYTPP--KQPLYNQDKTPEFIIKVLQFLAPMVILALA 125
            A+AM++E+YVGDID++T+P K K+ PP   + +  QDK+ +F+IK+LQFL P++IL LA
Sbjct: 67  TAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPLLILGLA 126

Query: 126 FGIHLYTKT 133
           FGI  YTKT
Sbjct: 127 FGIRYYTKT 135

[Close]

BLAST of Carg08903 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 177.2 bits (448), Expect = 8.9e-45
Identity = 80/126 (63.49%), Postives = 99/126 (78.57%), Query Frame = 0


Query: 6   VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFDVGHSS 65
           V +  EV+ H+   DCWLII  KVYDVT F++DHPGGDEVLLS+ GKDATNDF DVGHS 
Sbjct: 7   VLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVGHSD 66

Query: 66  AARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQFLAPMVILALAFG 125
            AR MM+++++G+IDSS++P  R Y  P+QP YNQDKTPEFIIK+LQFL P++IL LA  
Sbjct: 67  TARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILILGLALV 126

Query: 126 IHLYTK 132
           +  YTK
Sbjct: 127 VRHYTK 132

[Close]

BLAST of Carg08903 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 119.4 bits (298), Expect = 2.2e-27
Identity = 53/128 (41.41%), Postives = 85/128 (66.41%), Query Frame = 0


Query: 4   STVFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFDVGH 63
           + + +  +VA H  +NDCW++I  KVYD++ F+++HPGGD VLL+  GKDA+ DF DV H
Sbjct: 2   ANLISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNH 61

Query: 64  SSAARAMMEEFYVGDIDSSTIPTKRKYTPP--KQPLYNQDKTPEFIIKVLQFLAPMVILA 123
           S  A+ +M+++ +GD+D ST+P  ++Y PP  K+    +    E   K+L +L P++IL 
Sbjct: 62  SKDAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILG 121

Query: 124 LAFGIHLY 130
           +AF +  Y
Sbjct: 122 VAFALRFY 129

[Close]

BLAST of Carg08903 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 109.8 bits (273), Expect = 1.7e-24
Identity = 47/134 (35.07%), Postives = 92/134 (68.66%), Query Frame = 0


Query: 6   VFTLAEVASHDNRNDCWLIIEDKVYDVTKFLEDHPGGDEVLLSAIGKDATNDFFDVGHSS 65
           ++++ E A+H+ ++DCW++I+ KVYDV+ ++++HPGGD+VLL+  GKDAT+DF D GHS 
Sbjct: 7   LYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHSK 66

Query: 66  AARAMMEEFYVGDIDSSTIPTKRKYTPPKQPLYNQDKTPEFIIKVLQ-----FLAPMVIL 125
            AR +ME++++G++D S++P       P+  +Y +D+  + + K+       ++ P+ I+
Sbjct: 67  DARELMEKYFIGELDESSLP-----EIPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSII 126

Query: 126 ALAFGIH-LYTKTT 134
            ++  +  L+++ T
Sbjct: 127 TISVAVSVLFSRKT 135

The following BLAST results are available for this feature:

BLAST of Carg08903 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6606316.1	1.7e-69	100.00	hypothetical protein SDJN03_03633, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023534065.1	3.7e-69	99.25	cytochrome b5, seed isoform-like [Cucurbita pepo subsp. pepo]	[more]
XP_022931194.1	6.3e-69	97.74	cytochrome b5, seed isoform-like [Cucurbita moschata]	[more]
XP_022995981.1	2.4e-68	97.74	cytochrome b5, seed isoform-like [Cucurbita maxima]	[more]
XP_038889018.1	7.3e-65	92.48	cytochrome b5, seed isoform isoform X3 [Benincasa hispida]	[more]

BLAST of Carg08903 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49099	4.6e-54	76.56	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49098	3.0e-53	74.24	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O04354	7.3e-52	74.22	Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1	[more]
P49097	4.0e-50	70.99	Cytochrome b5 OS=Cuscuta reflexa OX=4129 PE=2 SV=1	[more]
P49100	5.2e-50	71.65	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]

BLAST of Carg08903 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ETM5	3.1e-69	97.74	cytochrome b5, seed isoform-like OS=Cucurbita moschata OX=3662 GN=LOC111437450 P...	[more]
A0A6J1K3E7	1.2e-68	97.74	cytochrome b5, seed isoform-like OS=Cucurbita maxima OX=3661 GN=LOC111491329 PE=...	[more]
A0A5A7UKQ8	2.3e-64	91.73	Cytochrome b5, seed isoform-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3CMW3	2.3e-64	91.73	cytochrome b5, seed isoform-like OS=Cucumis melo OX=3656 GN=LOC103502759 PE=3 SV...	[more]
A0A0A0LCD6	3.9e-64	91.73	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]

BLAST of Carg08903 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32720.1	1.2e-49	69.29	cytochrome B5 isoform B	[more]
AT5G48810.1	3.1e-45	62.02	cytochrome B5 isoform D	[more]
AT5G53560.1	8.9e-45	63.49	cytochrome B5 isoform E	[more]
AT2G46650.1	2.2e-27	41.41	cytochrome B5 isoform C	[more]
AT1G26340.1	1.7e-24	35.07	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 39..53 score: 53.94 coord: 54..61 score: 39.58 coord: 67..79 score: 36.6 coord: 29..39 score: 70.52
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 7..80 e-value: 5.4E-32 score: 122.3
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 8..79 e-value: 2.2E-25 score: 88.6
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 4..80 score: 28.557735
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 3..83 e-value: 1.8E-34 score: 119.7
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 5..90
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 4..131
None	No IPR available	PANTHER	PTHR19359:SF102	SUBFAMILY NOT NAMED	coord: 4..131
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 35..42

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08903-RA	Carg08903-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding