Carg08496 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg08496 vs. NCBI nr
Match: KAG7035551.1 (F-box protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 519.6 bits (1337), Expect = 1.4e-143
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0

Query: 1   MLRRKPPKIITRSTTAAAKSLVDNDDLLLHTILYDHQITKSFAFELWIPQTTKSRFTRLI 60
           MLRRKPPKIITRSTTAAAKSLVDNDDLLLHTILYDHQITKSFAFELWIPQTTKSRFTRLI
Sbjct: 1   MLRRKPPKIITRSTTAAAKSLVDNDDLLLHTILYDHQITKSFAFELWIPQTTKSRFTRLI 60

Query: 61  LGSGGPAARVHSPDRFIKEIDRGVYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASND 120
           LGSGGPAARVHSPDRFIKEIDRGVYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASND
Sbjct: 61  LGSGGPAARVHSPDRFIKEIDRGVYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASND 120

Query: 121 RGHKIVKYFGGCCGHLYWVEGVELEPMELIVYEMEDDHCGWFVKYRVDLKWVWSWYQNPG 180
           RGHKIVKYFGGCCGHLYWVEGVELEPMELIVYEMEDDHCGWFVKYRVDLKWVWSWYQNPG
Sbjct: 121 RGHKIVKYFGGCCGHLYWVEGVELEPMELIVYEMEDDHCGWFVKYRVDLKWVWSWYQNPG 180

Query: 181 SGGMEALYSKFTVLAIVDQSIVNAKSGSFEMDGFINIGRTSNTPHGFRTIEALLYIESLA 240
           SGGMEALYSKFTVLAIVDQSIVNAKSGSFEMDGFINIGRTSNTPHGFRTIEALLYIESLA
Sbjct: 181 SGGMEALYSKFTVLAIVDQSIVNAKSGSFEMDGFINIGRTSNTPHGFRTIEALLYIESLA 240

Query: 241 CV 243
           CV
Sbjct: 241 CV 242

BLAST of Carg08496 vs. NCBI nr
Match: KAG6605642.1 (Ethylene-responsive transcription factor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 232.6 bits (592), Expect = 3.5e-57
Identity = 102/104 (98.08%), Postives = 104/104 (100.00%), Query Frame = 0

Query: 98  KGIYFDLSEEKLHEFPVPEASNDRGHKIVKYFGGCCGHLYWVEGVELEPMELIVYEMEDD 157
           KGIYFDLSEEKL+EFPVPEASNDRGHKIVKYFGGCCGHLYWVEGVELEPMELIVYEMEDD
Sbjct: 179 KGIYFDLSEEKLYEFPVPEASNDRGHKIVKYFGGCCGHLYWVEGVELEPMELIVYEMEDD 238

Query: 158 HCGWFVKYRVDLKWVWSWYQNPGSGGMEALYSKFTVLAIVDQSI 202
           HCGWFVKYRVDLKWVWSWYQNPGSGGMEALYSKFTVLAIVDQS+
Sbjct: 239 HCGWFVKYRVDLKWVWSWYQNPGSGGMEALYSKFTVLAIVDQSM 282

BLAST of Carg08496 vs. NCBI nr
Match: XP_022958235.1 (F-box protein At5g07610-like [Cucurbita moschata])

HSP 1 Score: 190.3 bits (482), Expect = 2.0e-44
Identity = 98/186 (52.69%), Postives = 116/186 (62.37%), Query Frame = 0

Query: 76  FIKEIDRGVYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASNDRGHKIVKYFGGCCGH 135
           FI     GVYWNGA++W+RY    I  DL EEKLH   +P AS+  G + +KYFG  CG 
Sbjct: 209 FILGFSCGVYWNGALYWLRYLENCICLDLCEEKLHRLAIPRASDGWGDRTIKYFGNWCGR 268

Query: 136 LYWVEGVELEPMELIVYEMEDDH-CGWFVKYRVDLKWVWSWYQNPGSGGMEALYSKFTVL 195
           LYW+EGV+ E  ELIVYEM DD  CGW VKYRVDL+ V S + +P SG   AL SKF+VL
Sbjct: 269 LYWIEGVDSESTELIVYEMGDDRGCGWSVKYRVDLQRVCSLFPDPSSGVRAALNSKFSVL 328

Query: 196 AIVDQS------------------IVNAKSGSFEMDGFINIGRTSNTPHGFRTIEALLYI 243
           AIVDQS                   VN  SG FE   ++N GRT+NTPHG   I+A  YI
Sbjct: 329 AIVDQSEVGEEEPYIVLLSGGNAIRVNVMSGRFERMQYLNNGRTANTPHGVGLIDAHFYI 388

BLAST of Carg08496 vs. NCBI nr
Match: XP_023533019.1 (F-box protein At5g07610-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 185.7 bits (470), Expect = 4.9e-43
Identity = 94/187 (50.27%), Postives = 116/187 (62.03%), Query Frame = 0

Query: 76  FIKEIDRGVYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASNDRGHKIVKYFGGCCGH 135
           FI     GVYWNGA++W+RY    I  DL +EKLH   +P AS+  G + +KYFG  CG 
Sbjct: 209 FILGFSCGVYWNGALYWLRYLENCICLDLCQEKLHRLAIPRASDGWGDRTIKYFGNWCGR 268

Query: 136 LYWVEGVELEPMELIVYEMEDDH-CGWFVKYRVDLKWVWSWYQNPGSGGMEALYSKFTVL 195
           LYW+EGV+ +  E+IVYEM DD  CGW V+YRVDL+ V S + +P SG   AL SKF+VL
Sbjct: 269 LYWIEGVDSQSTEMIVYEMGDDRGCGWSVRYRVDLQRVCSLFPDPSSGVRAALNSKFSVL 328

Query: 196 AIVDQS-------------------IVNAKSGSFEMDGFINIGRTSNTPHGFRTIEALLY 243
           AIVDQS                    VN  SG FE   ++N GRT+NTPHG   I+A  Y
Sbjct: 329 AIVDQSEVGEEEEPYIVLLSGGNAIRVNVMSGRFERMEYLNNGRTANTPHGVGLIDAHFY 388

BLAST of Carg08496 vs. NCBI nr
Match: KAG7035550.1 (F-box protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 184.1 bits (466), Expect = 1.4e-42
Identity = 96/199 (48.24%), Postives = 116/199 (58.29%), Query Frame = 0

Query: 66  PAARVHSPDRFIKEIDRGVYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASNDRGHKI 125
           P     S   FI     GVYWNGA++W+RY    I  DL EEKLH   +P AS+    + 
Sbjct: 199 PCCEGSSKALFILGFSCGVYWNGALYWLRYLENCICLDLCEEKLHRLAIPRASDGWRDRT 258

Query: 126 VKYFGGCCGHLYWVEGVELEPMELIVYEMEDDH-CGWFVKYRVDLKWVWSWYQNPGSGGM 185
           +KYFG  CG LYW+EGV+ E  ELIVYEM DD  CGW +KYRVDL+ V + + +P SG  
Sbjct: 259 IKYFGNWCGRLYWIEGVDSESTELIVYEMGDDRGCGWSLKYRVDLQRVCNLFPDPSSGVR 318

Query: 186 EALYSKFTVLAIVDQS---------------------IVNAKSGSFEMDGFINIGRTSNT 243
            AL SKF+VL IVDQS                      VN  SG FE   ++N GRT+NT
Sbjct: 319 AALNSKFSVLTIVDQSEVGEEEEEEPYIVLLSGGNAIRVNVMSGRFERMQYLNNGRTANT 378

BLAST of Carg08496 vs. ExPASy Swiss-Prot
Match: Q9FLS0 (F-box protein At5g07610 OS=Arabidopsis thaliana OX=3702 GN=At5g07610 PE=2 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.9e-10
Identity = 34/104 (32.69%), Postives = 56/104 (53.85%), Query Frame = 0

Query: 72  SPDRFIKEIDRGVYWNGAVHWVRYCGKG-IYFDLSEEKLHEFPVPEASND----RGHKIV 131
           SP  FI E    V+WNGAV+W  +  +  + FD++ +++   P+P+  ++       +I+
Sbjct: 227 SPSTFI-EFSYSVFWNGAVNWYGFSSRDCLSFDINTQEIKILPLPDHEHEDEPLPDPRIL 286

Query: 132 KYFGGCCGHLYWVEGVELEPMELIVYEMEDDHCGWFVKYRVDLK 171
            +     G+LY++E        L VYEME +   W VKY VDL+
Sbjct: 287 MFLDESQGNLYYIEVNNQSSSNLRVYEMESNSSSWSVKYNVDLE 329

BLAST of Carg08496 vs. ExPASy TrEMBL
Match: A0A6J1H2Y4 (F-box protein At5g07610-like OS=Cucurbita moschata OX=3662 GN=LOC111459522 PE=4 SV=1)

HSP 1 Score: 190.3 bits (482), Expect = 9.6e-45
Identity = 98/186 (52.69%), Postives = 116/186 (62.37%), Query Frame = 0

Query: 76  FIKEIDRGVYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASNDRGHKIVKYFGGCCGH 135
           FI     GVYWNGA++W+RY    I  DL EEKLH   +P AS+  G + +KYFG  CG 
Sbjct: 209 FILGFSCGVYWNGALYWLRYLENCICLDLCEEKLHRLAIPRASDGWGDRTIKYFGNWCGR 268

Query: 136 LYWVEGVELEPMELIVYEMEDDH-CGWFVKYRVDLKWVWSWYQNPGSGGMEALYSKFTVL 195
           LYW+EGV+ E  ELIVYEM DD  CGW VKYRVDL+ V S + +P SG   AL SKF+VL
Sbjct: 269 LYWIEGVDSESTELIVYEMGDDRGCGWSVKYRVDLQRVCSLFPDPSSGVRAALNSKFSVL 328

Query: 196 AIVDQS------------------IVNAKSGSFEMDGFINIGRTSNTPHGFRTIEALLYI 243
           AIVDQS                   VN  SG FE   ++N GRT+NTPHG   I+A  YI
Sbjct: 329 AIVDQSEVGEEEPYIVLLSGGNAIRVNVMSGRFERMQYLNNGRTANTPHGVGLIDAHFYI 388

BLAST of Carg08496 vs. ExPASy TrEMBL
Match: A0A5A7TJJ2 (F-box protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00240 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 3.5e-31
Identity = 86/183 (46.99%), Postives = 105/183 (57.38%), Query Frame = 0

Query: 81  DRGVYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASNDRGHKIVKYFGGCCGHLYWVE 140
           D GVYWN AVHW+      +YFDL EEKLHE P+P        + V+YFG C G+L+ +E
Sbjct: 212 DCGVYWNNAVHWISTLENSLYFDLCEEKLHELPMPRVPYGLEERRVEYFGNCGGNLHLIE 271

Query: 141 GVELEPMELIVYEMEDDHCGWFVKYRVDLKWVWSWYQNPGSGGME---ALYSKFTVLAIV 200
             E E MEL VY+M+D+H GWFVKYRVDL+ V   +       M+     + KF+V AIV
Sbjct: 272 VYEAE-MELNVYQMQDNHSGWFVKYRVDLRGVSVGFPEMIPSVMDVDMGFFPKFSVAAIV 331

Query: 201 ---DQSI------------VNAKSGSFEMDGFI-NIGRTSNTP--HGFRTIEALLYIESL 243
              DQ              VN +SG FE  G I N GRTS  P   GF  I+A LYIE+L
Sbjct: 332 EGIDQEAYVVLEVEGKIVRVNVESGRFERLGQIENDGRTSEVPITVGFGRIDAFLYIENL 391

BLAST of Carg08496 vs. ExPASy TrEMBL
Match: A0A1S3ASN6 (F-box protein At5g07610-like OS=Cucumis melo OX=3656 GN=LOC103482573 PE=4 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 3.5e-31
Identity = 86/183 (46.99%), Postives = 105/183 (57.38%), Query Frame = 0

Query: 81  DRGVYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASNDRGHKIVKYFGGCCGHLYWVE 140
           D GVYWN AVHW+      +YFDL EEKLHE P+P        + V+YFG C G+L+ +E
Sbjct: 212 DCGVYWNNAVHWISTLENSLYFDLCEEKLHELPMPRVPYGLEERRVEYFGNCGGNLHLIE 271

Query: 141 GVELEPMELIVYEMEDDHCGWFVKYRVDLKWVWSWYQNPGSGGME---ALYSKFTVLAIV 200
             E E MEL VY+M+D+H GWFVKYRVDL+ V   +       M+     + KF+V AIV
Sbjct: 272 VYEAE-MELNVYQMQDNHSGWFVKYRVDLRGVSVGFPEMIPSVMDVDMGFFPKFSVAAIV 331

Query: 201 ---DQSI------------VNAKSGSFEMDGFI-NIGRTSNTP--HGFRTIEALLYIESL 243
              DQ              VN +SG FE  G I N GRTS  P   GF  I+A LYIE+L
Sbjct: 332 EGIDQEAYVVLEVEGKIVRVNVESGRFERLGQIENDGRTSEVPITVGFGRIDAFLYIENL 391

BLAST of Carg08496 vs. ExPASy TrEMBL
Match: A0A0A0KPG1 (F-box domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G167090 PE=4 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 6.0e-31
Identity = 91/239 (38.08%), Postives = 122/239 (51.05%), Query Frame = 0

Query: 24  NDDLLLHTILYDHQITKSFAFELWIPQTTKSRFTRLILGSGGPAARVHSPDRFIKEIDRG 83
           + D  +  + Y    T +F  E++   T          G   P   V S    ++  D G
Sbjct: 166 SSDYKVICVRYSDAYTDTFQIEMYSSVT----------GPWRPVQGVFSAPLSMR-FDSG 225

Query: 84  VYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASNDRGHKIVKYFGGCCGHLYWVEGVE 143
           VYWN AVHW+      +YFDL EEK+H+ P+P   + +  + VKYFG C G+L  +E  E
Sbjct: 226 VYWNNAVHWISTSENSLYFDLREEKVHDLPMPGVPDGQEQRRVKYFGTCGGNLNLIEIYE 285

Query: 144 LEPMELIVYEMEDDHCGWFVKYRVDLKWVWSWYQN--PGSGGME-ALYSKFTVLAI---- 203
            + MEL VY+M+DDHCGWFV+YRVDL+ V   +    P     +  L+ KF+V A+    
Sbjct: 286 AQEMELNVYQMQDDHCGWFVRYRVDLRGVSVVFPEMIPSEDDADLGLFPKFSVAAVVEGI 345

Query: 204 --------VDQSI--VNAKSGSFEMDGFINIGRTSN---TPHGFRTIEALLYIESLACV 243
                   VD  I  VN +SG FE  G I  G  S+      GF  I+A LYIESLA V
Sbjct: 346 EEASVVLEVDGKIVRVNVESGRFERLGEIEKGGCSSQLPLAVGFGRIDAFLYIESLASV 393

BLAST of Carg08496 vs. ExPASy TrEMBL
Match: A0A6J1I3L0 (F-box protein At5g07610 OS=Cucurbita maxima OX=3661 GN=LOC111468861 PE=4 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 2.3e-30
Identity = 80/183 (43.72%), Postives = 100/183 (54.64%), Query Frame = 0

Query: 81  DRGVYWNGAVHWVRYCGKGIYFDLSEEKLHEFPVPEASNDRGHKIVKYFGGCCGHLYWVE 140
           D GVYWN AVHW+      +YFDL EEKLHE P+P   +    + V+YFG C G+L+ +E
Sbjct: 220 DSGVYWNSAVHWIDAWENCLYFDLREEKLHELPMPRVPDGWEERRVEYFGSCGGNLHLIE 279

Query: 141 GVELEPMELIVYEMEDDHCGWFVKYRVDLKWVWSWYQN--PGSGGME-ALYSKFTVLAIV 200
             E + +E  VYEM+DDH GWFVKYRVDL  V   +    P    +E     KF+V+AIV
Sbjct: 280 NYEPQDLEFNVYEMKDDHSGWFVKYRVDLHCVSVAFPEIIPSEENLEFGFIPKFSVIAIV 339

Query: 201 DQS-----------------IVNAKSGSFE-MDGFINIGRTSNTPHGFRTIEALLYIESL 243
           D +                  VN +SG FE + G  N G  S     F  +EA LYIESL
Sbjct: 340 DGTEVGEESYIVLQIFEKIVRVNVESGRFERLVGSDNGGEISQVNGFFEQVEAYLYIESL 399

BLAST of Carg08496 vs. TAIR 10
Match: AT5G07610.1 (F-box family protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.4e-11
Identity = 34/104 (32.69%), Postives = 56/104 (53.85%), Query Frame = 0

Query: 72  SPDRFIKEIDRGVYWNGAVHWVRYCGKG-IYFDLSEEKLHEFPVPEASND----RGHKIV 131
           SP  FI E    V+WNGAV+W  +  +  + FD++ +++   P+P+  ++       +I+
Sbjct: 227 SPSTFI-EFSYSVFWNGAVNWYGFSSRDCLSFDINTQEIKILPLPDHEHEDEPLPDPRIL 286

Query: 132 KYFGGCCGHLYWVEGVELEPMELIVYEMEDDHCGWFVKYRVDLK 171
            +     G+LY++E        L VYEME +   W VKY VDL+
Sbjct: 287 MFLDESQGNLYYIEVNNQSSSNLRVYEMESNSSSWSVKYNVDLE 329