Carg08146 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg08146
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionD-3-phosphoglycerate dehydrogenase
LocationCarg_Chr04: 2519664 .. 2523330 (+)
RNA-Seq ExpressionCarg08146
SyntenyCarg08146
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACCTAAACCTTTCTCTCACCTTTACCTATTAAACCCTAGATCTTCCTCGTCGTCTCTCCTTTTCGTTCCGCCGGTGCTTTACATTTCTGAGTTCGGACGAGTAAATGGCTGCCTCTGCTTCGCCTTCGCTCCGTACGTCGACGGTTCGGAGCTCGGTACTTTCTTCTCTCTCTTCTAAGCTTCCTCTTTCTTCGGCTTTTTCTGTTAATTTTCGTCCTCGGAGTGTTGGTGTGCCGCGGTTTGTTGTGTTGGTTTCTGCGTCGTTGAATGCGAAACCGACGGTTCTTGTTGCGGAGAAGCTTGGTGAAGCGGGTGTTGATCTCCTTAAGGAGTTTGCTAATGTGGATTGCTCCTATAATTTGACTCCTGAGGAGCTCTGCACTAAGATCTCGCTCTGTGATGCGCTTATTGTGCGGAGTGGGACTAAGGTCAGCCGTGAAGTGTTTGAATCCTCTGGAGGGAGGTTGAAGGTTGTTGGGAGGGCTGGTGTTGGCATTGATAATGTGGATCTGGCGGCCGCCACTGAGCATGGTTGCTTGGTGGTGAATGCTCCGACTGCGAACACGGTTGCTGCCGCTGAGCACGGAATCGCGCTTTTAACTGCAATGGCCAGAAATGTTGCCCAAGCCGATGCGTCTGTCAAAGCCGGTACGTTTGTATAAAACATTTTACGGAATCTTCGTGTTCTGTAACTTCTTGTTTGCTTTGGATCTGCTCGAAACCATCGAAAGGGCGAAAAGTTCGGAAAGATCTCGACCTTTTGTCGTAATATTGGACGAACATTTTGTTTGAATCATTTTTTAAAATACATTTTTAATGTTTAAAGTTGATTGCGCTCTAAAGAAACTCCTTGCCCGATTGTGGTGGTTCTGGGAATACGTCATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGCATTGAGATGCACTGTAAAAGGTGGGAACCTGTCGGGAATTTGTAAGTTCAGAATTATTCCCATGCTTGCCTGGCCTGGATTTTACCGGACTTTTTACGATTAATAATTTTTCTCTGTAACATAGATTTAAGTAACTTTTTTCTGCATCAGACGTTCTCTTTCCCATATCAGCCAGTTGACACGAAATAGTCGATGATTTTATAGTTTATTGGTTTGATTTTGTATACAAGATGCGTTTGTTAAAAGAACTACTTATGATTTTACATTTTCGCCTCCACCCTAGGGAAGTGGCAACGCAACAAATATGTCGGTGTATCACTTGTGGGGAAAACACTGGCTGTAATGGGATTCGGCAAAGTTGGATCTGAAGTTGCTCGTCGTGCAAAGGGTCTTGGCATGCATGTAATTGCCCATGACCCATATGCCCCAGCTGATCGTGCTCGTGCGATAGGCGTAGAACTTGTGAGCTTTGATGAAGCCATTGGAACTGCGGATTTCATCTCTTTACATATGCCTTTAACTCCATCCACGTCAAAGATTCTTAACGATGAAAATTTTGCAAAAATGAAGAAAGGAGTTAGGATTGTGAATGTTGCTCGTGGAGGAGTAATTGATGAAGAAGCTCTTGTGAGGGCGCTGGATGCTGGAATTGTTGCTCAGGTTCCTTTCCTTCCTACAACTTGAAACTGAAGTTTGCTATTATTATTTACAACGCAAGGATTGTAATATGTTCTTTTTCTGATAGGCTGCACTTGATGTTTTTACTGTGGAACCACCTCCTAAGGATAGCAAGTTGATTCAGCATGAGAATGTCACTGTAACTCCTCATCTCGGTGCCAGCACAATGGAGGCTCAGGTGTGTGTTCTTTTAAATCAAGGACTTAAAGCTTGTGTTTTTCTTTTTACAAGTTCTAATGATCTTTGAATTTGGCGTGCAGGAAGGAGTTGCGGTTGAAATAGCTGAAGCTGTTGTTGGAGCCCTGAAAGGGGAGCTTGCAGCTACTGCCGTCAATGCTCCGATGGTTCCTGCAGAGGTATAAGCCCTTAGTTTTGGAATCTTCTATCTCTCATGTTTCATATTTCATGGTCACTTAAATGTTCATCTGAAAGTAGTTTATTTTTGTTAAAAAAAAAAGAAAAAAGTTTGCCTTCTAGTTCTGTTCATGTTGACCTCTTTACTTCTCATCGTCATTTGTTTTCTTGATTCTCTTGCCTCCCAAGCGGCTATATCTTTGGTTTTCTGGTTCATTATGCTATCGATAGAGTAAGAATCTTCTGTTGAACGCTTATCATTTATATGACTTAGAAACCTTTGACATTTCTCCCGATTTCTCGACACTGAATATGGTGTTGTAAAAAAGAAAAAAAAAAAAAGGCTAAATAGTAATCCTTCAACAGTAGGAGAGAATAGAGATTACTTTTGACATTTCTCTAGGTATTGGGATGATTTTTCAGAAAACTGTTAAATGTAAATGGTTCTTAAGCTATCAAAACCTTACTTCTAATTCCAACAATATAACAGTGCCACTATAAATGGATTACTTTGTTGTTTCTTTTAATTCGTTACTTTTCGCATACTTATTATCAAGATTTGCTGGGTGACAATTGTGATTTGCATCATTATTCATGTACATTTTATTTTCTGTTAACTAATTTCTCAAGTTTCTGGTATATATAAGGATTCTAAGTTAGTATTATTTCTTCCCTACAGGTGTTAACCGAGCTGAAACCATTTGTTGAACTCGCTGAGAAATTGGGAAGACTGGCTGTCCAATTGGTAGCTGGAGGTAGTGGTGTGAAAACTGTGAAAGTGACATATGCATCCTCTAGAGCTCCTGATGATCTTGACACCAGACTTCTTCGTGCCATGATAACCAAGGGTCTAATCGAGCCGATTTCGAGTGTTTTTGTGAACTTGGTGAATGCTGACTTTACTGCCAAACAGAGAGGACTAAGGATAAGTGAGGAGCGAGTCGTTCTGGATGGGTCACCTGAGAAACCATTAGAATACATTCAGGTCCAGATTGCCAACGTGGAATCCAGATTTGCCAGTGCAATATCAGACACCGGGGAAATTAAGGTGGAAGGACTTGTGAAAGATGGAATCCCTCACCTGACGAAGGTCGGTTCCTTCGAAGTCGACGTTAGTCTGGAAGGTAGCATCATACTGTGCAGGCAGGTTGACCAGCCAGGTATGATTGGAAGAGTTGGAAGCGTTCTGGGAGAAGAAAATGTTAATGTGAGCTTCATGAGTGTCGGGAGGATTGCTCCTCGAAAGCAGGCAGTCATGGCGATCGGTGTCGATGAACAGCCCAGCAAACAAGCTTTGAAGAGGATTGGTGACATTCCAGCAATTGAAGAGTTTGTTTTCCTCAAGTTGTAGGATGGTGGTGGTCATGAACTTGAAGGCCCCCCCTATCTTCTGGGTATTTGGATACATTACATTCCTACGTGTTTGGCCCCAATTTTGACAGTTTTCGTTTAAATAAGCTGGTTGGTTTGTTTTCTTTACTAAGTTTGAAGAGCGAGTCTTTATTCACTACGAACGGGATCCCTTTTAAATGGATTCTTGGTCGGAGTTTAATGGCCCATAATAGGATGGATCAAATCCAAATGTAAGGACCTAGATCCATTGCTATTCTTTTGGGCTTTAAAACGTGTCTGTTAGGGGAAAGTTTCCACACCCTTATAAATGGTGTTTTGTTCTCTTTGGAAACGTCAGGGAAAGGTTTCTACACTCTTATAAAT

mRNA sequence

AACCTAAACCTTTCTCTCACCTTTACCTATTAAACCCTAGATCTTCCTCGTCGTCTCTCCTTTTCGTTCCGCCGGTGCTTTACATTTCTGAGTTCGGACGAGTAAATGGCTGCCTCTGCTTCGCCTTCGCTCCGTACGTCGACGGTTCGGAGCTCGGTACTTTCTTCTCTCTCTTCTAAGCTTCCTCTTTCTTCGGCTTTTTCTGTTAATTTTCGTCCTCGGAGTGTTGGTGTGCCGCGGTTTGTTGTGTTGGTTTCTGCGTCGTTGAATGCGAAACCGACGGTTCTTGTTGCGGAGAAGCTTGGTGAAGCGGGTGTTGATCTCCTTAAGGAGTTTGCTAATGTGGATTGCTCCTATAATTTGACTCCTGAGGAGCTCTGCACTAAGATCTCGCTCTGTGATGCGCTTATTGTGCGGAGTGGGACTAAGGTCAGCCGTGAAGTGTTTGAATCCTCTGGAGGGAGGTTGAAGGTTGTTGGGAGGGCTGGTGTTGGCATTGATAATGTGGATCTGGCGGCCGCCACTGAGCATGGTTGCTTGGTGGTGAATGCTCCGACTGCGAACACGGTTGCTGCCGCTGAGCACGGAATCGCGCTTTTAACTGCAATGGCCAGAAATGTTGCCCAAGCCGATGCGTCTGTCAAAGCCGGGAAGTGGCAACGCAACAAATATGTCGGTGTATCACTTGTGGGGAAAACACTGGCTGTAATGGGATTCGGCAAAGTTGGATCTGAAGTTGCTCGTCGTGCAAAGGGTCTTGGCATGCATGTAATTGCCCATGACCCATATGCCCCAGCTGATCGTGCTCGTGCGATAGGCGTAGAACTTGTGAGCTTTGATGAAGCCATTGGAACTGCGGATTTCATCTCTTTACATATGCCTTTAACTCCATCCACGTCAAAGATTCTTAACGATGAAAATTTTGCAAAAATGAAGAAAGGAGTTAGGATTGTGAATGTTGCTCGTGGAGGAGTAATTGATGAAGAAGCTCTTGTGAGGGCGCTGGATGCTGGAATTGTTGCTCAGGCTGCACTTGATGTTTTTACTGTGGAACCACCTCCTAAGGATAGCAAGTTGATTCAGCATGAGAATGTCACTGTAACTCCTCATCTCGGTGCCAGCACAATGGAGGCTCAGGAAGGAGTTGCGGTTGAAATAGCTGAAGCTGTTGTTGGAGCCCTGAAAGGGGAGCTTGCAGCTACTGCCGTCAATGCTCCGATGGTTCCTGCAGAGGTGTTAACCGAGCTGAAACCATTTGTTGAACTCGCTGAGAAATTGGGAAGACTGGCTGTCCAATTGGTAGCTGGAGGTAGTGGTGTGAAAACTGTGAAAGTGACATATGCATCCTCTAGAGCTCCTGATGATCTTGACACCAGACTTCTTCGTGCCATGATAACCAAGGGTCTAATCGAGCCGATTTCGAGTGTTTTTGTGAACTTGGTGAATGCTGACTTTACTGCCAAACAGAGAGGACTAAGGATAAGTGAGGAGCGAGTCGTTCTGGATGGGTCACCTGAGAAACCATTAGAATACATTCAGGTCCAGATTGCCAACGTGGAATCCAGATTTGCCAGTGCAATATCAGACACCGGGGAAATTAAGGTGGAAGGACTTGTGAAAGATGGAATCCCTCACCTGACGAAGGTCGGTTCCTTCGAAGTCGACGTTAGTCTGGAAGGTAGCATCATACTGTGCAGGCAGGTTGACCAGCCAGGTATGATTGGAAGAGTTGGAAGCGTTCTGGGAGAAGAAAATGTTAATGTGAGCTTCATGAGTGTCGGGAGGATTGCTCCTCGAAAGCAGGCAGTCATGGCGATCGGTGTCGATGAACAGCCCAGCAAACAAGCTTTGAAGAGGATTGGTGACATTCCAGCAATTGAAGAGTTTGTTTTCCTCAAGTTGTAGGATGGTGGTGGTCATGAACTTGAAGGCCCCCCCTATCTTCTGGGTATTTGGATACATTACATTCCTACGTGTTTGGCCCCAATTTTGACAGTTTTCGTTTAAATAAGCTGGTTGGTTTGTTTTCTTTACTAAGTTTGAAGAGCGAGTCTTTATTCACTACGAACGGGATCCCTTTTAAATGGATTCTTGGTCGGAGTTTAATGGCCCATAATAGGATGGATCAAATCCAAATGTAAGGACCTAGATCCATTGCTATTCTTTTGGGCTTTAAAACGTGTCTGTTAGGGGAAAGTTTCCACACCCTTATAAATGGTGTTTTGTTCTCTTTGGAAACGTCAGGGAAAGGTTTCTACACTCTTATAAAT

Coding sequence (CDS)

ATGGCTGCCTCTGCTTCGCCTTCGCTCCGTACGTCGACGGTTCGGAGCTCGGTACTTTCTTCTCTCTCTTCTAAGCTTCCTCTTTCTTCGGCTTTTTCTGTTAATTTTCGTCCTCGGAGTGTTGGTGTGCCGCGGTTTGTTGTGTTGGTTTCTGCGTCGTTGAATGCGAAACCGACGGTTCTTGTTGCGGAGAAGCTTGGTGAAGCGGGTGTTGATCTCCTTAAGGAGTTTGCTAATGTGGATTGCTCCTATAATTTGACTCCTGAGGAGCTCTGCACTAAGATCTCGCTCTGTGATGCGCTTATTGTGCGGAGTGGGACTAAGGTCAGCCGTGAAGTGTTTGAATCCTCTGGAGGGAGGTTGAAGGTTGTTGGGAGGGCTGGTGTTGGCATTGATAATGTGGATCTGGCGGCCGCCACTGAGCATGGTTGCTTGGTGGTGAATGCTCCGACTGCGAACACGGTTGCTGCCGCTGAGCACGGAATCGCGCTTTTAACTGCAATGGCCAGAAATGTTGCCCAAGCCGATGCGTCTGTCAAAGCCGGGAAGTGGCAACGCAACAAATATGTCGGTGTATCACTTGTGGGGAAAACACTGGCTGTAATGGGATTCGGCAAAGTTGGATCTGAAGTTGCTCGTCGTGCAAAGGGTCTTGGCATGCATGTAATTGCCCATGACCCATATGCCCCAGCTGATCGTGCTCGTGCGATAGGCGTAGAACTTGTGAGCTTTGATGAAGCCATTGGAACTGCGGATTTCATCTCTTTACATATGCCTTTAACTCCATCCACGTCAAAGATTCTTAACGATGAAAATTTTGCAAAAATGAAGAAAGGAGTTAGGATTGTGAATGTTGCTCGTGGAGGAGTAATTGATGAAGAAGCTCTTGTGAGGGCGCTGGATGCTGGAATTGTTGCTCAGGCTGCACTTGATGTTTTTACTGTGGAACCACCTCCTAAGGATAGCAAGTTGATTCAGCATGAGAATGTCACTGTAACTCCTCATCTCGGTGCCAGCACAATGGAGGCTCAGGAAGGAGTTGCGGTTGAAATAGCTGAAGCTGTTGTTGGAGCCCTGAAAGGGGAGCTTGCAGCTACTGCCGTCAATGCTCCGATGGTTCCTGCAGAGGTGTTAACCGAGCTGAAACCATTTGTTGAACTCGCTGAGAAATTGGGAAGACTGGCTGTCCAATTGGTAGCTGGAGGTAGTGGTGTGAAAACTGTGAAAGTGACATATGCATCCTCTAGAGCTCCTGATGATCTTGACACCAGACTTCTTCGTGCCATGATAACCAAGGGTCTAATCGAGCCGATTTCGAGTGTTTTTGTGAACTTGGTGAATGCTGACTTTACTGCCAAACAGAGAGGACTAAGGATAAGTGAGGAGCGAGTCGTTCTGGATGGGTCACCTGAGAAACCATTAGAATACATTCAGGTCCAGATTGCCAACGTGGAATCCAGATTTGCCAGTGCAATATCAGACACCGGGGAAATTAAGGTGGAAGGACTTGTGAAAGATGGAATCCCTCACCTGACGAAGGTCGGTTCCTTCGAAGTCGACGTTAGTCTGGAAGGTAGCATCATACTGTGCAGGCAGGTTGACCAGCCAGGTATGATTGGAAGAGTTGGAAGCGTTCTGGGAGAAGAAAATGTTAATGTGAGCTTCATGAGTGTCGGGAGGATTGCTCCTCGAAAGCAGGCAGTCATGGCGATCGGTGTCGATGAACAGCCCAGCAAACAAGCTTTGAAGAGGATTGGTGACATTCCAGCAATTGAAGAGTTTGTTTTCCTCAAGTTGTAG

Protein sequence

MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTVLVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVKAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVELVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRALDAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALKGELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQIANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIGRVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL
Homology
BLAST of Carg08146 vs. NCBI nr
Match: XP_023541369.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6600311.1 D-3-phosphoglycerate dehydrogenase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030970.1 D-3-phosphoglycerate dehydrogenase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1115.5 bits (2884), Expect = 0.0e+00
Identity = 599/599 (100.00%), Postives = 599/599 (100.00%), Query Frame = 0

Query: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60
           MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL
Sbjct: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 599

BLAST of Carg08146 vs. NCBI nr
Match: XP_022942525.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 598/599 (99.83%), Postives = 599/599 (100.00%), Query Frame = 0

Query: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60
           MAASASPSLRTSTVR+SVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPSLRTSTVRNSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL
Sbjct: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 599

BLAST of Carg08146 vs. NCBI nr
Match: XP_022979807.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 597/599 (99.67%), Postives = 598/599 (99.83%), Query Frame = 0

Query: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60
           MAASASPSLRTS +RSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPSLRTSKLRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL
Sbjct: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 599

BLAST of Carg08146 vs. NCBI nr
Match: XP_008456163.1 (PREDICTED: D-3-phosphoglycerate dehydrogenase 1, chloroplastic [Cucumis melo] >KAA0037176.1 D-3-phosphoglycerate dehydrogenase 1 [Cucumis melo var. makuwa] >TYK13897.1 D-3-phosphoglycerate dehydrogenase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 572/599 (95.49%), Postives = 592/599 (98.83%), Query Frame = 0

Query: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60
           MAASASP+LRT ++R+S+LSS SSKLPL SAFSVN RPRSVG PRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLRTPSLRTSLLSSFSSKLPLPSAFSVNLRPRSVGAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAG+DLLKEFANVDCSYNL+PEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LV FDEAI TADF+SLHMPLTP+TSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVGFDEAIATADFLSLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRI+EERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISD+GEIKVEGLVKDG+PHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDSGEIKVEGLVKDGVPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           +VGS+LGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSK++LKRIGD+PAIEEFVFLKL
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRIGDVPAIEEFVFLKL 599

BLAST of Carg08146 vs. NCBI nr
Match: XP_004140713.1 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic [Cucumis sativus] >KGN57501.1 hypothetical protein Csa_011422 [Cucumis sativus])

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 571/599 (95.33%), Postives = 591/599 (98.66%), Query Frame = 0

Query: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60
           MAASASP+LRT ++R+S+ SSLSSKLPL SAFSVN RPRSV  PRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLRTPSLRNSLFSSLSSKLPLPSAFSVNLRPRSVAAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAG+DLLKEFANVDCSYNL+PEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LV F+EAI TADFISLHMPLTP+TSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVGFEEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRI+EERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISD+GEIKVEGLVKDG+PHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDSGEIKVEGLVKDGVPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           +VGS+LGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSK++LKRIGD+PAIEEFVFLKL
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRIGDVPAIEEFVFLKL 599

BLAST of Carg08146 vs. ExPASy Swiss-Prot
Match: O49485 (D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH1 PE=1 SV=1)

HSP 1 Score: 921.4 bits (2380), Expect = 5.3e-267
Identity = 493/601 (82.03%), Postives = 547/601 (91.01%), Query Frame = 0

Query: 2   AASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVG-VPRFVVLVSASL--NAKP 61
           AAS+S ++ T+++R+  LSS S   PL SA SV F  R    + R +VLVS S    +KP
Sbjct: 6   AASSSIAVATNSLRNVTLSSRS---PLPSAISVAFPSRGRNTLQRRLVLVSCSTGDGSKP 65

Query: 62  TVLVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSG 121
           T+LVAEKLG+AG+ LL++ ANVDCSYN+TPEEL  KISLCDALIVRSGTKV REVFESS 
Sbjct: 66  TILVAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFESSH 125

Query: 122 GRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADAS 181
           GRLKVVGRAGVGIDNVDL+AATE GCLVVNAPTANT+AAAEHGIAL+ AMARNVAQADAS
Sbjct: 126 GRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADAS 185

Query: 182 VKAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIG 241
           VKAG+W+RNKYVGVSLVGKTLAV+GFGKVG+EVARRAKGLGM VIAHDPYAPADRA AIG
Sbjct: 186 VKAGEWKRNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAHAIG 245

Query: 242 VELVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVR 301
           V+LVSFDEA+ TADFISLHMPLTP+TSKILNDE FAKMKKGVRIVNVARGGVIDE+ALVR
Sbjct: 246 VDLVSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDALVR 305

Query: 302 ALDAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGA 361
           ALDAGIVAQAALDVFT EPP KDSKL+QHE VTVTPHLGASTMEAQEGVA+EIAEAVVGA
Sbjct: 306 ALDAGIVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVVGA 365

Query: 362 LKGELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAP 421
           L GELAATAVNAPMV AEVLTELKP+V LAEKLGRLAVQLVAGGSGVK  K+TYAS+RA 
Sbjct: 366 LNGELAATAVNAPMVSAEVLTELKPYVVLAEKLGRLAVQLVAGGSGVKNAKITYASARAT 425

Query: 422 DDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQ 481
           DDLDTRLLRAMITKG+IEPIS V+VNLVNADFTAKQRGLR+SEERV+LDGSPE PLE I 
Sbjct: 426 DDLDTRLLRAMITKGIIEPISDVYVNLVNADFTAKQRGLRLSEERVLLDGSPESPLETIT 485

Query: 482 VQIANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGM 541
           VQ++NVES+FAS++S++GE+KVEG VKDG+PHLTKVGSFEVDV+LEGSIILCRQVDQPGM
Sbjct: 486 VQLSNVESKFASSLSESGEVKVEGKVKDGVPHLTKVGSFEVDVTLEGSIILCRQVDQPGM 545

Query: 542 IGRVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLK 600
           IG VGS+LGE NVNV+FMSVGRIAPRKQA+MAIGVD+ PSK+ LK+IG+IPA+EEFVFLK
Sbjct: 546 IGTVGSILGESNVNVNFMSVGRIAPRKQAIMAIGVDDIPSKETLKKIGEIPAVEEFVFLK 603

BLAST of Carg08146 vs. ExPASy Swiss-Prot
Match: Q9LT69 (D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH3 PE=1 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 6.3e-260
Identity = 475/592 (80.24%), Postives = 530/592 (89.53%), Query Frame = 0

Query: 9   LRTSTVRSSVLSSLSSKLPL-SSAFSVNFRPRSVGVPRFVVLVSASLNAKPTVLVAEKLG 68
           + TS   SS+ SS S  +   SS F +  R R +     V   S+    KPT+LV EKLG
Sbjct: 1   MATSLNLSSIFSSSSRLVTTPSSVFPIRQRRRII----LVTSSSSGGGGKPTILVTEKLG 60

Query: 69  EAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGRLKVVGRA 128
           +AG+DLLK++ANVDCSY+L+ EELCTKISLCDALIVRSGTKV R+VFESS GRLKVVGRA
Sbjct: 61  QAGIDLLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVGRA 120

Query: 129 GVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVKAGKWQRN 188
           GVGIDNVDLAAATE+GCLVVNAPTANTVAAAEHGIALLTAMARN+AQADAS+KAGKW RN
Sbjct: 121 GVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWTRN 180

Query: 189 KYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVELVSFDEA 248
           KYVGVSLVGKTLAV+GFGKVGSEVARRA+GLGMHVI HDPYAPADRARAIGVELVSF+ A
Sbjct: 181 KYVGVSLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFEVA 240

Query: 249 IGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRALDAGIVAQ 308
           I TADFISLH+PLT +TSK++ND  FA MKKGVRIVNVARGGVIDEEAL+RALD+GIVAQ
Sbjct: 241 ISTADFISLHLPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIVAQ 300

Query: 309 AALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALKGELAATA 368
           AALDVFTVEPP KD+KL+ HE+VT TPHLGASTMEAQEGV++E+AEAV+GAL+GELAATA
Sbjct: 301 AALDVFTVEPPVKDNKLVLHESVTATPHLGASTMEAQEGVSIEVAEAVIGALRGELAATA 360

Query: 369 VNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDDLDTRLLR 428
           VNAPMVP EVL ELKP+V LAEKLGRLAVQLV GGSGV  VKVTYASSRAPDDLDTRLLR
Sbjct: 361 VNAPMVPLEVLRELKPYVVLAEKLGRLAVQLVTGGSGVNAVKVTYASSRAPDDLDTRLLR 420

Query: 429 AMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQIANVESR 488
           AM+ KG+IEPISSVF+NLVN+D+ AKQRG++ISEER+VLDGSPE P+EYI V+IANVESR
Sbjct: 421 AMVIKGIIEPISSVFINLVNSDYIAKQRGVKISEERMVLDGSPENPIEYITVRIANVESR 480

Query: 489 FASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIGRVGSVLG 548
           FASA+S++GEIKVEG VK G+P LTKVG F VDVSLEGS+ILCRQVDQPGMIG+V S+LG
Sbjct: 481 FASALSESGEIKVEGRVKQGVPSLTKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASILG 540

Query: 549 EENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           +ENVNVSFMSVGRIAP KQAVMAIGVDEQPSK+ LK+IGDIPAIEEFVFLKL
Sbjct: 541 DENVNVSFMSVGRIAPGKQAVMAIGVDEQPSKETLKKIGDIPAIEEFVFLKL 588

BLAST of Carg08146 vs. ExPASy Swiss-Prot
Match: O04130 (D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PGDH2 PE=1 SV=2)

HSP 1 Score: 833.6 bits (2152), Expect = 1.4e-240
Identity = 427/543 (78.64%), Postives = 490/543 (90.24%), Query Frame = 0

Query: 57  KPTVLVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFES 116
           KP +LV EKLGEAGV+LL+EF +VDCSY+L+PE+L  K++  DALIVRSGTKV+REVFE+
Sbjct: 82  KPRILVTEKLGEAGVNLLREFGDVDCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEA 141

Query: 117 SGGRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQAD 176
           + GRLKVVGRAGVGIDNVDL AATEHGCLVVNAPTANTVAAAEHGIALL +MARNVAQAD
Sbjct: 142 AKGRLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQAD 201

Query: 177 ASVKAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARA 236
           AS+KAGKW+R+KYVGVSLVGKTLAVMGFGKVG+EVARRAKGLGM VI+HDPYAPADRARA
Sbjct: 202 ASIKAGKWERSKYVGVSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHDPYAPADRARA 261

Query: 237 IGVELVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEAL 296
           +GV+LVSFD+AI TADF+SLHMPLTP+T K+ NDE F+KMKKGVR++NVARGGVIDE+AL
Sbjct: 262 LGVDLVSFDQAISTADFVSLHMPLTPATKKVFNDETFSKMKKGVRLINVARGGVIDEDAL 321

Query: 297 VRALDAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVV 356
           VRALDAGIVAQAALDVF  EPP KDS+LIQHENVTVTPHLGAST EAQEGVA+EIAEAV 
Sbjct: 322 VRALDAGIVAQAALDVFCEEPPSKDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVA 381

Query: 357 GALKGELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSR 416
           GALKGEL+ATAVNAPMV  EVL+EL P++ LAEKLGRLAVQL +GG GV++++V Y S+R
Sbjct: 382 GALKGELSATAVNAPMVAPEVLSELTPYIVLAEKLGRLAVQLASGGKGVQSIRVVYRSAR 441

Query: 417 APDDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEY 476
             DDLDTRLLRAMITKG+IEPIS  +VNLVNADF AKQ+GLRISEER+V+D SPE P++ 
Sbjct: 442 DRDDLDTRLLRAMITKGIIEPISDSYVNLVNADFIAKQKGLRISEERMVVDSSPEYPVDS 501

Query: 477 IQVQIANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQP 536
           IQVQI NVES FA A+SD G+I +EG VK G+PHLT VGSF VDVSLEG++ILCRQVDQP
Sbjct: 502 IQVQILNVESNFAGAVSDAGDISIEGKVKYGVPHLTCVGSFGVDVSLEGNLILCRQVDQP 561

Query: 537 GMIGRVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVF 596
           GMIG+VG++LGE+NVNV+FMSVGR   RKQA+MAIGVDE+P  + L+RIG + AIEEFVF
Sbjct: 562 GMIGQVGNILGEQNVNVNFMSVGRTVLRKQAIMAIGVDEEPDNKTLERIGGVSAIEEFVF 621

Query: 597 LKL 600
           LKL
Sbjct: 622 LKL 624

BLAST of Carg08146 vs. ExPASy Swiss-Prot
Match: Q58424 (D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) OX=243232 GN=serA PE=3 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 5.0e-108
Identity = 228/541 (42.14%), Postives = 332/541 (61.37%), Query Frame = 0

Query: 60  VLVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGG 119
           +LV + L E  + +L+E   V+ +  LT EEL  KI   D L+VRSGTKV+R+V E +  
Sbjct: 4   ILVTDPLHEDAIKILEEVGEVEVATGLTKEELLEKIKDADVLVVRSGTKVTRDVIEKA-E 63

Query: 120 RLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASV 179
           +LKV+GRAGVG+DN+D+ AATE G +VVNAP A++++ AE  + L+ A ARN+ QA AS+
Sbjct: 64  KLKVIGRAGVGVDNIDVEAATEKGIIVVNAPDASSISVAELTMGLMLAAARNIPQATASL 123

Query: 180 KAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGV 239
           K G+W R ++ G+ L GKTL V+G G++G +V +RAK  GM++I +DPY P + A ++GV
Sbjct: 124 KRGEWDRKRFKGIELYGKTLGVIGLGRIGQQVVKRAKAFGMNIIGYDPYIPKEVAESMGV 183

Query: 240 ELV-SFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVR 299
           ELV   +E    ADFI+LH+PLTP T  I+  E  A MKK   IVN ARGG+IDE+AL  
Sbjct: 184 ELVDDINELCKRADFITLHVPLTPKTRHIIGREQIALMKKNAIIVNCARGGLIDEKALYE 243

Query: 300 ALDAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGA 359
           AL  G +  AALDVF  E PPKD+ L+  +NV  TPH GAST EAQ+     +AE +   
Sbjct: 244 ALKEGKIRAAALDVFE-EEPPKDNPLLTLDNVIGTPHQGASTEEAQKAAGTIVAEQIKKV 303

Query: 360 LKGELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAP 419
           L+GELA   VN P +P E L +LKP++ LAE LG + +Q++ G   V  V++ Y+   A 
Sbjct: 304 LRGELAENVVNMPNIPQEKLGKLKPYMLLAEMLGNIVMQVLDG--SVNRVELIYSGELAK 363

Query: 420 DDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQ 479
           +  D  L++    KGL+ PI    +NLVNA   AK R + + E       S EK    I+
Sbjct: 364 EKTD--LIKRAFLKGLLSPILLAGINLVNAPIIAKNRNINVVESST----SEEKYGNAIK 423

Query: 480 VQIANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGM 539
           +          +A SD  +  + G + +  P + +V  +EV    EG + + + +D+PG 
Sbjct: 424 I----------TAESDKKKFSIVGAIINNKPVILEVDGYEVSFIPEGVLAIIKHIDRPGT 483

Query: 540 IGRVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLK 599
           IGRV   LG+  +N++ M VGR  P  ++VM + +D    ++ +++I +IP I++   + 
Sbjct: 484 IGRVCITLGDYGINIASMQVGRKEPGGESVMLLNLDHTVPEEVIEKIKEIPNIKDVAVIN 524

BLAST of Carg08146 vs. ExPASy Swiss-Prot
Match: O27051 (D-3-phosphoglycerate dehydrogenase OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=serA PE=3 SV=1)

HSP 1 Score: 375.9 bits (964), Expect = 8.3e-103
Identity = 212/542 (39.11%), Postives = 330/542 (60.89%), Query Frame = 0

Query: 60  VLVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGG 119
           VL+A+ + E G+  L+E A V  +  +TPEEL   I   DA++VRS TKV+REV E++  
Sbjct: 6   VLIADSINEKGISELEEVAEVVVNTTITPEELLDAIKDFDAIVVRSRTKVTREVIEAA-P 65

Query: 120 RLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASV 179
           RLK++ RAGVG+DNVD+ AAT+ G +V+NAP + ++  AEH I L+ A+AR +A AD SV
Sbjct: 66  RLKIIARAGVGVDNVDVKAATDRGIMVINAPESTSITVAEHSIGLMLALARKIAIADRSV 125

Query: 180 KAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGV 239
           K GKW++N+++G+ L GKTL ++G G++GS+V  R K  GM ++ +DPY   + A  +GV
Sbjct: 126 KEGKWEKNRFMGIELNGKTLGIIGMGRIGSQVVVRTKAFGMDIMVYDPYISKEAAEEMGV 185

Query: 240 ELVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRA 299
            +   +  +  +D +++H+PLTP T  +++++ F  MK    IVN ARGG+IDE+AL RA
Sbjct: 186 TVTDLETLLRESDIVTIHVPLTPETRHLISEDEFKLMKDTAFIVNCARGGIIDEDALYRA 245

Query: 300 LDAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGAL 359
           L  G +A AALDVF  E PP+ S L++ ENV +TPH+GAST EAQ   A+ +A  +    
Sbjct: 246 LKDGEIAGAALDVFE-EEPPEGSPLLELENVVLTPHIGASTSEAQRDAAIIVANEIKTVF 305

Query: 360 KGELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPD 419
           +G      +N P++ +E    LKP++ELAEK+G +  Q + G   ++ + VTY    A  
Sbjct: 306 QGGAPRNVLNMPVMDSETYKSLKPYIELAEKMGAIIAQALPG--NIEKLDVTYCGELAEM 365

Query: 420 DLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISE-ERVVLDGSPEKPLEYIQ 479
             D  +L   + + ++ PI +  VNL+NA   AK+RG+ ++E  R   DG       Y  
Sbjct: 366 QFD--ILTRTMLQAILNPILTEPVNLINAPSIAKKRGIMVTEARRSESDG-------YRS 425

Query: 480 VQIANVESRFASAISDTGEIKVEGL-VKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPG 539
           + IA  E       SD G   VE   +K+  P +  +  + VDV  EG++I+ R  D PG
Sbjct: 426 IIIATAE-------SDRGGFSVEATHIKE--PTIIGINGYRVDVKPEGTMIIARYRDLPG 485

Query: 540 MIGRVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFL 599
            IG +G+ LG+  +N++ M VGR     +AVM + VD+    + ++ +  +  +++ V +
Sbjct: 486 TIGAIGTKLGQHGINIATMQVGRKEIGGEAVMVLKVDQSVPAEVIEEVKKLDNVDDAVAI 525

BLAST of Carg08146 vs. ExPASy TrEMBL
Match: A0A6J1FWG6 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111447534 PE=3 SV=1)

HSP 1 Score: 1114.4 bits (2881), Expect = 0.0e+00
Identity = 598/599 (99.83%), Postives = 599/599 (100.00%), Query Frame = 0

Query: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60
           MAASASPSLRTSTVR+SVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPSLRTSTVRNSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL
Sbjct: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 599

BLAST of Carg08146 vs. ExPASy TrEMBL
Match: A0A6J1IPQ1 (D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111479396 PE=3 SV=1)

HSP 1 Score: 1112.1 bits (2875), Expect = 0.0e+00
Identity = 597/599 (99.67%), Postives = 598/599 (99.83%), Query Frame = 0

Query: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60
           MAASASPSLRTS +RSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPSLRTSKLRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL
Sbjct: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 599

BLAST of Carg08146 vs. ExPASy TrEMBL
Match: A0A5D3CPP5 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold832G00870 PE=3 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 572/599 (95.49%), Postives = 592/599 (98.83%), Query Frame = 0

Query: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60
           MAASASP+LRT ++R+S+LSS SSKLPL SAFSVN RPRSVG PRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLRTPSLRTSLLSSFSSKLPLPSAFSVNLRPRSVGAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAG+DLLKEFANVDCSYNL+PEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LV FDEAI TADF+SLHMPLTP+TSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVGFDEAIATADFLSLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRI+EERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISD+GEIKVEGLVKDG+PHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDSGEIKVEGLVKDGVPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           +VGS+LGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSK++LKRIGD+PAIEEFVFLKL
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRIGDVPAIEEFVFLKL 599

BLAST of Carg08146 vs. ExPASy TrEMBL
Match: A0A1S3C2L0 (D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103496183 PE=3 SV=1)

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 572/599 (95.49%), Postives = 592/599 (98.83%), Query Frame = 0

Query: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60
           MAASASP+LRT ++R+S+LSS SSKLPL SAFSVN RPRSVG PRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLRTPSLRTSLLSSFSSKLPLPSAFSVNLRPRSVGAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAG+DLLKEFANVDCSYNL+PEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LV FDEAI TADF+SLHMPLTP+TSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVGFDEAIATADFLSLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRI+EERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISD+GEIKVEGLVKDG+PHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDSGEIKVEGLVKDGVPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           +VGS+LGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSK++LKRIGD+PAIEEFVFLKL
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRIGDVPAIEEFVFLKL 599

BLAST of Carg08146 vs. ExPASy TrEMBL
Match: A0A0A0LBW6 (D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G199630 PE=3 SV=1)

HSP 1 Score: 1078.9 bits (2789), Expect = 0.0e+00
Identity = 571/599 (95.33%), Postives = 591/599 (98.66%), Query Frame = 0

Query: 1   MAASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVGVPRFVVLVSASLNAKPTV 60
           MAASASP+LRT ++R+S+ SSLSSKLPL SAFSVN RPRSV  PRFVVLVSASLNAKPTV
Sbjct: 1   MAASASPTLRTPSLRNSLFSSLSSKLPLPSAFSVNLRPRSVAAPRFVVLVSASLNAKPTV 60

Query: 61  LVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120
           LVAEKLGEAG+DLLKEFANVDCSYNL+PEELCTKISLCDALIVRSGTKVSREVFESSGGR
Sbjct: 61  LVAEKLGEAGLDLLKEFANVDCSYNLSPEELCTKISLCDALIVRSGTKVSREVFESSGGR 120

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK
Sbjct: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180

Query: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240
           AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE
Sbjct: 181 AGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVE 240

Query: 241 LVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300
           LV F+EAI TADFISLHMPLTP+TSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL
Sbjct: 241 LVGFEEAIATADFISLHMPLTPATSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRAL 300

Query: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360
           DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK
Sbjct: 301 DAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALK 360

Query: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420
           GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD
Sbjct: 361 GELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDD 420

Query: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQ 480
           LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRI+EERV++DGSPEKPLEYIQVQ
Sbjct: 421 LDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRITEERVIIDGSPEKPLEYIQVQ 480

Query: 481 IANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540
           IANVES+FASAISD+GEIKVEGLVKDG+PHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG
Sbjct: 481 IANVESKFASAISDSGEIKVEGLVKDGVPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIG 540

Query: 541 RVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           +VGS+LGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSK++LKRIGD+PAIEEFVFLKL
Sbjct: 541 KVGSILGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKESLKRIGDVPAIEEFVFLKL 599

BLAST of Carg08146 vs. TAIR 10
Match: AT4G34200.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 921.4 bits (2380), Expect = 3.8e-268
Identity = 493/601 (82.03%), Postives = 547/601 (91.01%), Query Frame = 0

Query: 2   AASASPSLRTSTVRSSVLSSLSSKLPLSSAFSVNFRPRSVG-VPRFVVLVSASL--NAKP 61
           AAS+S ++ T+++R+  LSS S   PL SA SV F  R    + R +VLVS S    +KP
Sbjct: 6   AASSSIAVATNSLRNVTLSSRS---PLPSAISVAFPSRGRNTLQRRLVLVSCSTGDGSKP 65

Query: 62  TVLVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSG 121
           T+LVAEKLG+AG+ LL++ ANVDCSYN+TPEEL  KISLCDALIVRSGTKV REVFESS 
Sbjct: 66  TILVAEKLGDAGIKLLEDVANVDCSYNMTPEELNIKISLCDALIVRSGTKVGREVFESSH 125

Query: 122 GRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADAS 181
           GRLKVVGRAGVGIDNVDL+AATE GCLVVNAPTANT+AAAEHGIAL+ AMARNVAQADAS
Sbjct: 126 GRLKVVGRAGVGIDNVDLSAATEFGCLVVNAPTANTIAAAEHGIALMAAMARNVAQADAS 185

Query: 182 VKAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIG 241
           VKAG+W+RNKYVGVSLVGKTLAV+GFGKVG+EVARRAKGLGM VIAHDPYAPADRA AIG
Sbjct: 186 VKAGEWKRNKYVGVSLVGKTLAVLGFGKVGTEVARRAKGLGMRVIAHDPYAPADRAHAIG 245

Query: 242 VELVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVR 301
           V+LVSFDEA+ TADFISLHMPLTP+TSKILNDE FAKMKKGVRIVNVARGGVIDE+ALVR
Sbjct: 246 VDLVSFDEALATADFISLHMPLTPTTSKILNDETFAKMKKGVRIVNVARGGVIDEDALVR 305

Query: 302 ALDAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGA 361
           ALDAGIVAQAALDVFT EPP KDSKL+QHE VTVTPHLGASTMEAQEGVA+EIAEAVVGA
Sbjct: 306 ALDAGIVAQAALDVFTKEPPAKDSKLVQHERVTVTPHLGASTMEAQEGVAIEIAEAVVGA 365

Query: 362 LKGELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAP 421
           L GELAATAVNAPMV AEVLTELKP+V LAEKLGRLAVQLVAGGSGVK  K+TYAS+RA 
Sbjct: 366 LNGELAATAVNAPMVSAEVLTELKPYVVLAEKLGRLAVQLVAGGSGVKNAKITYASARAT 425

Query: 422 DDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQ 481
           DDLDTRLLRAMITKG+IEPIS V+VNLVNADFTAKQRGLR+SEERV+LDGSPE PLE I 
Sbjct: 426 DDLDTRLLRAMITKGIIEPISDVYVNLVNADFTAKQRGLRLSEERVLLDGSPESPLETIT 485

Query: 482 VQIANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGM 541
           VQ++NVES+FAS++S++GE+KVEG VKDG+PHLTKVGSFEVDV+LEGSIILCRQVDQPGM
Sbjct: 486 VQLSNVESKFASSLSESGEVKVEGKVKDGVPHLTKVGSFEVDVTLEGSIILCRQVDQPGM 545

Query: 542 IGRVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLK 600
           IG VGS+LGE NVNV+FMSVGRIAPRKQA+MAIGVD+ PSK+ LK+IG+IPA+EEFVFLK
Sbjct: 546 IGTVGSILGESNVNVNFMSVGRIAPRKQAIMAIGVDDIPSKETLKKIGEIPAVEEFVFLK 603

BLAST of Carg08146 vs. TAIR 10
Match: AT3G19480.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 897.9 bits (2319), Expect = 4.4e-261
Identity = 475/592 (80.24%), Postives = 530/592 (89.53%), Query Frame = 0

Query: 9   LRTSTVRSSVLSSLSSKLPL-SSAFSVNFRPRSVGVPRFVVLVSASLNAKPTVLVAEKLG 68
           + TS   SS+ SS S  +   SS F +  R R +     V   S+    KPT+LV EKLG
Sbjct: 1   MATSLNLSSIFSSSSRLVTTPSSVFPIRQRRRII----LVTSSSSGGGGKPTILVTEKLG 60

Query: 69  EAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFESSGGRLKVVGRA 128
           +AG+DLLK++ANVDCSY+L+ EELCTKISLCDALIVRSGTKV R+VFESS GRLKVVGRA
Sbjct: 61  QAGIDLLKKYANVDCSYDLSLEELCTKISLCDALIVRSGTKVGRDVFESSRGRLKVVGRA 120

Query: 129 GVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVKAGKWQRN 188
           GVGIDNVDLAAATE+GCLVVNAPTANTVAAAEHGIALLTAMARN+AQADAS+KAGKW RN
Sbjct: 121 GVGIDNVDLAAATEYGCLVVNAPTANTVAAAEHGIALLTAMARNIAQADASIKAGKWTRN 180

Query: 189 KYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARAIGVELVSFDEA 248
           KYVGVSLVGKTLAV+GFGKVGSEVARRA+GLGMHVI HDPYAPADRARAIGVELVSF+ A
Sbjct: 181 KYVGVSLVGKTLAVLGFGKVGSEVARRARGLGMHVITHDPYAPADRARAIGVELVSFEVA 240

Query: 249 IGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEALVRALDAGIVAQ 308
           I TADFISLH+PLT +TSK++ND  FA MKKGVRIVNVARGGVIDEEAL+RALD+GIVAQ
Sbjct: 241 ISTADFISLHLPLTAATSKMMNDVTFAMMKKGVRIVNVARGGVIDEEALLRALDSGIVAQ 300

Query: 309 AALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVVGALKGELAATA 368
           AALDVFTVEPP KD+KL+ HE+VT TPHLGASTMEAQEGV++E+AEAV+GAL+GELAATA
Sbjct: 301 AALDVFTVEPPVKDNKLVLHESVTATPHLGASTMEAQEGVSIEVAEAVIGALRGELAATA 360

Query: 369 VNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSRAPDDLDTRLLR 428
           VNAPMVP EVL ELKP+V LAEKLGRLAVQLV GGSGV  VKVTYASSRAPDDLDTRLLR
Sbjct: 361 VNAPMVPLEVLRELKPYVVLAEKLGRLAVQLVTGGSGVNAVKVTYASSRAPDDLDTRLLR 420

Query: 429 AMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEYIQVQIANVESR 488
           AM+ KG+IEPISSVF+NLVN+D+ AKQRG++ISEER+VLDGSPE P+EYI V+IANVESR
Sbjct: 421 AMVIKGIIEPISSVFINLVNSDYIAKQRGVKISEERMVLDGSPENPIEYITVRIANVESR 480

Query: 489 FASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQPGMIGRVGSVLG 548
           FASA+S++GEIKVEG VK G+P LTKVG F VDVSLEGS+ILCRQVDQPGMIG+V S+LG
Sbjct: 481 FASALSESGEIKVEGRVKQGVPSLTKVGLFGVDVSLEGSVILCRQVDQPGMIGKVASILG 540

Query: 549 EENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           +ENVNVSFMSVGRIAP KQAVMAIGVDEQPSK+ LK+IGDIPAIEEFVFLKL
Sbjct: 541 DENVNVSFMSVGRIAPGKQAVMAIGVDEQPSKETLKKIGDIPAIEEFVFLKL 588

BLAST of Carg08146 vs. TAIR 10
Match: AT1G17745.1 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 833.6 bits (2152), Expect = 1.0e-241
Identity = 427/543 (78.64%), Postives = 490/543 (90.24%), Query Frame = 0

Query: 57  KPTVLVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFES 116
           KP +LV EKLGEAGV+LL+EF +VDCSY+L+PE+L  K++  DALIVRSGTKV+REVFE+
Sbjct: 82  KPRILVTEKLGEAGVNLLREFGDVDCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEA 141

Query: 117 SGGRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQAD 176
           + GRLKVVGRAGVGIDNVDL AATEHGCLVVNAPTANTVAAAEHGIALL +MARNVAQAD
Sbjct: 142 AKGRLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQAD 201

Query: 177 ASVKAGKWQRNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPADRARA 236
           AS+KAGKW+R+KYVGVSLVGKTLAVMGFGKVG+EVARRAKGLGM VI+HDPYAPADRARA
Sbjct: 202 ASIKAGKWERSKYVGVSLVGKTLAVMGFGKVGTEVARRAKGLGMTVISHDPYAPADRARA 261

Query: 237 IGVELVSFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEAL 296
           +GV+LVSFD+AI TADF+SLHMPLTP+T K+ NDE F+KMKKGVR++NVARGGVIDE+AL
Sbjct: 262 LGVDLVSFDQAISTADFVSLHMPLTPATKKVFNDETFSKMKKGVRLINVARGGVIDEDAL 321

Query: 297 VRALDAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVV 356
           VRALDAGIVAQAALDVF  EPP KDS+LIQHENVTVTPHLGAST EAQEGVA+EIAEAV 
Sbjct: 322 VRALDAGIVAQAALDVFCEEPPSKDSRLIQHENVTVTPHLGASTKEAQEGVAIEIAEAVA 381

Query: 357 GALKGELAATAVNAPMVPAEVLTELKPFVELAEKLGRLAVQLVAGGSGVKTVKVTYASSR 416
           GALKGEL+ATAVNAPMV  EVL+EL P++ LAEKLGRLAVQL +GG GV++++V Y S+R
Sbjct: 382 GALKGELSATAVNAPMVAPEVLSELTPYIVLAEKLGRLAVQLASGGKGVQSIRVVYRSAR 441

Query: 417 APDDLDTRLLRAMITKGLIEPISSVFVNLVNADFTAKQRGLRISEERVVLDGSPEKPLEY 476
             DDLDTRLLRAMITKG+IEPIS  +VNLVNADF AKQ+GLRISEER+V+D SPE P++ 
Sbjct: 442 DRDDLDTRLLRAMITKGIIEPISDSYVNLVNADFIAKQKGLRISEERMVVDSSPEYPVDS 501

Query: 477 IQVQIANVESRFASAISDTGEIKVEGLVKDGIPHLTKVGSFEVDVSLEGSIILCRQVDQP 536
           IQVQI NVES FA A+SD G+I +EG VK G+PHLT VGSF VDVSLEG++ILCRQVDQP
Sbjct: 502 IQVQILNVESNFAGAVSDAGDISIEGKVKYGVPHLTCVGSFGVDVSLEGNLILCRQVDQP 561

Query: 537 GMIGRVGSVLGEENVNVSFMSVGRIAPRKQAVMAIGVDEQPSKQALKRIGDIPAIEEFVF 596
           GMIG+VG++LGE+NVNV+FMSVGR   RKQA+MAIGVDE+P  + L+RIG + AIEEFVF
Sbjct: 562 GMIGQVGNILGEQNVNVNFMSVGRTVLRKQAIMAIGVDEEPDNKTLERIGGVSAIEEFVF 621

Query: 597 LKL 600
           LKL
Sbjct: 622 LKL 624

BLAST of Carg08146 vs. TAIR 10
Match: AT1G17745.2 (D-3-phosphoglycerate dehydrogenase )

HSP 1 Score: 813.1 bits (2099), Expect = 1.4e-235
Identity = 426/570 (74.74%), Postives = 490/570 (85.96%), Query Frame = 0

Query: 57  KPTVLVAEKLGEAGVDLLKEFANVDCSYNLTPEELCTKISLCDALIVRSGTKVSREVFES 116
           KP +LV EKLGEAGV+LL+EF +VDCSY+L+PE+L  K++  DALIVRSGTKV+REVFE+
Sbjct: 82  KPRILVTEKLGEAGVNLLREFGDVDCSYDLSPEDLKKKVAESDALIVRSGTKVTREVFEA 141

Query: 117 SGGRLKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQAD 176
           + GRLKVVGRAGVGIDNVDL AATEHGCLVVNAPTANTVAAAEHGIALL +MARNVAQAD
Sbjct: 142 AKGRLKVVGRAGVGIDNVDLQAATEHGCLVVNAPTANTVAAAEHGIALLASMARNVAQAD 201

Query: 177 ASVKAG-----------KW----------------QRNKYVGVSLVGKTLAVMGFGKVGS 236
           AS+KAG           +W                +R+KYVGVSLVGKTLAVMGFGKVG+
Sbjct: 202 ASIKAGTLNYLFLVLLLRWNCRQSKHQYTIETETEKRSKYVGVSLVGKTLAVMGFGKVGT 261

Query: 237 EVARRAKGLGMHVIAHDPYAPADRARAIGVELVSFDEAIGTADFISLHMPLTPSTSKILN 296
           EVARRAKGLGM VI+HDPYAPADRARA+GV+LVSFD+AI TADF+SLHMPLTP+T K+ N
Sbjct: 262 EVARRAKGLGMTVISHDPYAPADRARALGVDLVSFDQAISTADFVSLHMPLTPATKKVFN 321

Query: 297 DENFAKMKKGVRIVNVARGGVIDEEALVRALDAGIVAQAALDVFTVEPPPKDSKLIQHEN 356
           DE F+KMKKGVR++NVARGGVIDE+ALVRALDAGIVAQAALDVF  EPP KDS+LIQHEN
Sbjct: 322 DETFSKMKKGVRLINVARGGVIDEDALVRALDAGIVAQAALDVFCEEPPSKDSRLIQHEN 381

Query: 357 VTVTPHLGASTMEAQEGVAVEIAEAVVGALKGELAATAVNAPMVPAEVLTELKPFVELAE 416
           VTVTPHLGAST EAQEGVA+EIAEAV GALKGEL+ATAVNAPMV  EVL+EL P++ LAE
Sbjct: 382 VTVTPHLGASTKEAQEGVAIEIAEAVAGALKGELSATAVNAPMVAPEVLSELTPYIVLAE 441

Query: 417 KLGRLAVQLVAGGSGVKTVKVTYASSRAPDDLDTRLLRAMITKGLIEPISSVFVNLVNAD 476
           KLGRLAVQL +GG GV++++V Y S+R  DDLDTRLLRAMITKG+IEPIS  +VNLVNAD
Sbjct: 442 KLGRLAVQLASGGKGVQSIRVVYRSARDRDDLDTRLLRAMITKGIIEPISDSYVNLVNAD 501

Query: 477 FTAKQRGLRISEERVVLDGSPEKPLEYIQVQIANVESRFASAISDTGEIKVEGLVKDGIP 536
           F AKQ+GLRISEER+V+D SPE P++ IQVQI NVES FA A+SD G+I +EG VK G+P
Sbjct: 502 FIAKQKGLRISEERMVVDSSPEYPVDSIQVQILNVESNFAGAVSDAGDISIEGKVKYGVP 561

Query: 537 HLTKVGSFEVDVSLEGSIILCRQVDQPGMIGRVGSVLGEENVNVSFMSVGRIAPRKQAVM 596
           HLT VGSF VDVSLEG++ILCRQVDQPGMIG+VG++LGE+NVNV+FMSVGR   RKQA+M
Sbjct: 562 HLTCVGSFGVDVSLEGNLILCRQVDQPGMIGQVGNILGEQNVNVNFMSVGRTVLRKQAIM 621

Query: 597 AIGVDEQPSKQALKRIGDIPAIEEFVFLKL 600
           AIGVDE+P  + L+RIG + AIEEFVFLKL
Sbjct: 622 AIGVDEEPDNKTLERIGGVSAIEEFVFLKL 651

BLAST of Carg08146 vs. TAIR 10
Match: AT5G14780.1 (formate dehydrogenase )

HSP 1 Score: 115.5 bits (288), Expect = 1.4e-25
Identity = 79/246 (32.11%), Postives = 126/246 (51.22%), Query Frame = 0

Query: 121 LKVVGRAGVGIDNVDLAAATEHGCLVVNAPTANTVAAAEHGIALLTAMARNVAQADASVK 180
           LK++  AG+G D++DL AA   G  V     +N V+ AE  +  +  + RN       V 
Sbjct: 120 LKLLLTAGIGSDHIDLQAAAAAGLTVAEVTGSNVVSVAEDELMRILILMRNFVPGYNQVV 179

Query: 181 AGKWQ--RNKYVGVSLVGKTLAVMGFGKVGSEVARRAKGLGMHVIAHDPYAPA-DRARAI 240
            G+W      Y    L GKT+  +G G++G  + +R K  G +++ HD    A +  +  
Sbjct: 180 KGEWNVAGIAYRAYDLEGKTIGTVGAGRIGKLLLQRLKPFGCNLLYHDRLQMAPELEKET 239

Query: 241 GVELV-SFDEAIGTADFISLHMPLTPSTSKILNDENFAKMKKGVRIVNVARGGVIDEEAL 300
           G + V   +E +   D I ++MPLT  T  + N E   K+KKGV IVN ARG +++ +A+
Sbjct: 240 GAKFVEDLNEMLPKCDVIVINMPLTEKTRGMFNKELIGKLKKGVLIVNNARGAIMERQAV 299

Query: 301 VRALDAGIVAQAALDVFTVEPPPKDSKLIQHENVTVTPHLGASTMEAQEGVAVEIAEAVV 360
           V A+++G +   + DV+  +P PKD       N  +TPH   +T++AQ   A    + + 
Sbjct: 300 VDAVESGHIGGYSGDVWDPQPAPKDHPWRYMPNQAMTPHTSGTTIDAQLRYAAGTKDMLE 359

Query: 361 GALKGE 363
              KGE
Sbjct: 360 RYFKGE 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023541369.10.0e+00100.00D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita pepo subsp. ... [more]
XP_022942525.10.0e+0099.83D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita moschata][more]
XP_022979807.10.0e+0099.67D-3-phosphoglycerate dehydrogenase 1, chloroplastic-like [Cucurbita maxima][more]
XP_008456163.10.0e+0095.49PREDICTED: D-3-phosphoglycerate dehydrogenase 1, chloroplastic [Cucumis melo] >K... [more]
XP_004140713.10.0e+0095.33D-3-phosphoglycerate dehydrogenase 1, chloroplastic [Cucumis sativus] >KGN57501.... [more]
Match NameE-valueIdentityDescription
O494855.3e-26782.03D-3-phosphoglycerate dehydrogenase 1, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q9LT696.3e-26080.24D-3-phosphoglycerate dehydrogenase 3, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
O041301.4e-24078.64D-3-phosphoglycerate dehydrogenase 2, chloroplastic OS=Arabidopsis thaliana OX=3... [more]
Q584245.0e-10842.14D-3-phosphoglycerate dehydrogenase OS=Methanocaldococcus jannaschii (strain ATCC... [more]
O270518.3e-10339.11D-3-phosphoglycerate dehydrogenase OS=Methanothermobacter thermautotrophicus (st... [more]
Match NameE-valueIdentityDescription
A0A6J1FWG60.0e+0099.83D-3-phosphoglycerate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111447534... [more]
A0A6J1IPQ10.0e+0099.67D-3-phosphoglycerate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111479396 P... [more]
A0A5D3CPP50.0e+0095.49D-3-phosphoglycerate dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C2L00.0e+0095.49D-3-phosphoglycerate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103496183 PE=3 ... [more]
A0A0A0LBW60.0e+0095.33D-3-phosphoglycerate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_3G199630 PE... [more]
Match NameE-valueIdentityDescription
AT4G34200.13.8e-26882.03D-3-phosphoglycerate dehydrogenase [more]
AT3G19480.14.4e-26180.24D-3-phosphoglycerate dehydrogenase [more]
AT1G17745.11.0e-24178.64D-3-phosphoglycerate dehydrogenase [more]
AT1G17745.21.4e-23574.74D-3-phosphoglycerate dehydrogenase [more]
AT5G14780.11.4e-2532.11formate dehydrogenase [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015878S-adenosyl-L-homocysteine hydrolase, NAD binding domainSMARTSM00997AdoHcyase_NAD_2coord: 187..356
e-value: 9.3E-4
score: -31.0
IPR029009Allosteric substrate binding domain superfamilyGENE3D3.30.1330.90coord: 373..525
e-value: 1.5E-30
score: 107.8
IPR029009Allosteric substrate binding domain superfamilySUPERFAMILY143548Serine metabolism enzymes domaincoord: 375..521
IPR002912ACT domainPFAMPF01842ACTcoord: 527..589
e-value: 4.8E-9
score: 35.8
IPR002912ACT domainPROSITEPS51671ACTcoord: 527..599
score: 9.606108
NoneNo IPR availableGENE3D3.30.70.260coord: 526..599
e-value: 1.6E-20
score: 74.8
NoneNo IPR availableGENE3D3.40.50.720coord: 153..335
e-value: 1.8E-110
score: 370.4
NoneNo IPR availableGENE3D3.40.50.720coord: 62..362
e-value: 1.8E-110
score: 370.4
NoneNo IPR availablePANTHERPTHR42938FORMATE DEHYDROGENASE 1coord: 36..594
NoneNo IPR availablePANTHERPTHR42938:SF35D-3-PHOSPHOGLYCERATE DEHYDROGENASEcoord: 36..594
NoneNo IPR availableCDDcd04902ACT_3PGDH-xctcoord: 528..599
e-value: 8.38955E-22
score: 87.5253
NoneNo IPR availableCDDcd12173PGDH_4coord: 60..362
e-value: 1.09027E-163
score: 467.278
NoneNo IPR availableSUPERFAMILY52283Formate/glycerate dehydrogenase catalytic domain-likecoord: 57..190
NoneNo IPR availableSUPERFAMILY55021ACT-likecoord: 517..595
IPR006140D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domainPFAMPF028262-Hacid_dh_Ccoord: 162..337
e-value: 4.6E-63
score: 211.8
IPR006236D-3-phosphoglycerate dehydrogenaseTIGRFAMTIGR01327TIGR01327coord: 60..598
e-value: 2.0E-169
score: 562.7
IPR006139D-isomer specific 2-hydroxyacid dehydrogenase, catalytic domainPFAMPF003892-Hacid_dhcoord: 60..369
e-value: 6.2E-35
score: 120.5
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00671D_2_HYDROXYACID_DH_3coord: 276..292
IPR029753D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved sitePROSITEPS00670D_2_HYDROXYACID_DH_2coord: 247..269
IPR029752D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding domain conserved site 1PROSITEPS00065D_2_HYDROXYACID_DH_1coord: 199..226
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 156..338

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg08146-RACarg08146-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006564 L-serine biosynthetic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0051287 NAD binding
molecular_function GO:0004617 phosphoglycerate dehydrogenase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor