Carg08101 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg08101
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Aspartate--tRNA ligase
Location	Carg_Chr04: 2277843 .. 2282772 (-)
RNA-Seq Expression	Carg08101
Synteny	Carg08101

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCACTCGGCCGTCTTCATCCACACGCAGAGTCTTCTTGTTCACCTTTCCTCTGCGAGCGCTCCGTTTCCAGAATCCTTAAACCCTTCCGCCTAACTTCGCCGCTTCCATCAATCTTCCATTGATCCACCTTCAAACCTTCCGCCATGTCCCGCTCTATTCTCCGAGCTCTCCCTTTCTCCACCCTTCGCCACAAGCCTCCTCAATTTCTCCCCATCTTCCACCGTATTTCTCATCTCCGCCTCGCGAAATCCATTTCCAGAACTCTGAGACCTATTTCCTCACTCTCTGTTTCCGCCACTCATCCGCCTTCCTCCGCCACCGAAACGCTCGTTTCCAGTTCGAAGTCTCCGTCGCCTCTTAAGGATTCTCTTCAGTGGATCAATCGTACTGCGTTCTGTGGTGAATTGTCGGCTGGCGATGTTGGGAAGCGGGTTCGGCTTTGTGGTTGGGTTGCACTACATAGGGTTCATGGTGGTTTGACTTTTCTCAATCTTAGAGACCACAGTGGTATCGTACAGGTTTGTTACTTCTGTTTGAATTTTTGCGATTAGCTTGAAGTTGAAGAAGCTTTATTGTTGAGTGTTGATTTTTACTGCTTGAATTAGGTGACTACGCTACCTAACGAGTTTCCTGATGCTCATTCGGTTATTAACGACTTGCGGCTCGAGTATGTTGTTGCTATAGAAGGCATTGTACGGTCTCGACCTGATGATTCGATTAATGTGAAAATGAAAACTGGCTTGATAGAGGTATTTATGAATTTCGATTTCATTTTACTCCTCATTTTGCCAGGCATTTGGTTAGAAAGATTTTTAGATTTCTCGCTTATTGCATTATGCTCATGCCTGCTCCCCACGGCCCCTTTAGCCAGCTCCTGAATGAACCAGAATTTTAAGTTATTAAGTCCTCAATCTCGGACTTGAACCTAGTATCTGGGAGGAATTTCCAAACATCCCAAGTCTTTGCAAAATAAATATGTTAATTCTTAGTGGCAATAATCCTGGGAGTTCTTGATCATGGTTGTTAAAACTCGAGATAGGCTAGTCGTGGTATAATTTTGTAGTTCTTTTGGGATATCTTGGTGCTTCCAAATAAATTCTGATTCTACAGTGGTACACCTGCGTGGTGTTTAGCTCAACTCAAAGGCAAGAATTCTAAGCTTGTAGCAATTAATCCAATTTTTAAAGCTTTAACGGAGTGAAACTCCATGGTTTTTTGAAGAAGGGGCGAGAGATTGGGTTCGAGCCTGTGATTTAGTTGACTTTATGGGCTCTTCATGGATTATTCTCACTAGTTCATTTTGTATTATAATGGTTTCGTGATCAATTTGAATTGAAGTGACTTTTTATAAGTTCCCATGGTTGCCTTTCTTGTAGCTTCTCAATTATTGGATGAAAAGTAACGTGTCTTACTAAGTGAAGGGATATGGGTTGTAGGGTAGTTGATGCCGATTGAAGTAGTATGGAGTTCGAAACTTTTTAACTTTGTTATCTTAGTTCTCTTCTCTAGGACTATGTAAGTTTCCTATGACGCAACTATCATTCTATTTTTTCAGTTTTTGTTCTGATAATTTCTTGATATATTATAGGTTGCTGCAGAGCATGTTCAAATCTTAAATTCTGTTGGATCAAAGTTACCTTTTCTAGTTACAATTGCTGATGATGCAAAGGATTCTGCTAAAGAGGAAATTCGATTGAGGTTGTTATTTCATTCTATCATTTCAGGCCAAGTTCTAATTTATATTTGTTCACTCAAACCAGATGCACAGTTTGATCCCATATTCTACGTGCTTAATTGAATCAAATGTCTGAAATCATTTGATCATCATTCTTCTTTTTTGGGTTGGGGGGAGTGAGTTAGGCGCACAGAAAAATTTCAACTTCCTTCCCTTCTTAGATCTTTCTTTAGACTTTCATTGAAGGGTGAATGCCGTGAAATAAGTTCTCTCCCTTATCTAAGATGCCCATGGGTTTGAGATGATCACATTTTTTATTTTCTCGGAACCAAATGCTATCTGGTTTTAGATTATAAGGAGTTGAGTATTGATAGGTACCGATATCTTGATCTTCGGAGGAAGCAAATGAATTCGAACATAATGCTTCGGCATAAAGTGGTGAAACTCATTCGAAGATATTTGGAGGATGCACATGACTTTGTGGAGGTACTGCCCTGTTTATAACTTTTAACTGACCGTTGACCCTTTTTTCTGAAAATTTCTTGAATCATTCTCTGCATTAGAACTTCACATTGTACTAAATAAATGGTTAGAAATTGAATTTGTTCTAGCCTTATTTCCACTTTGAACTTTTGTTGGTTATAAGGAAGATTGAGACGCCAATACTTTCTAGATCTACTCCAGAAGGTGCTCGAGATTATTTGGTGCCGTCTAGAGTTCAGGTAGCCTACAAATCCTCAACTTCTGTTGATTTAATTCTCTCAAGGCAGGTTATTTTGGTAACTAACTGGTATTTTTTGATCCAGAAACTTAAAATAAATTTCTTAAGATATCTGAATGTGATGCCATTTTCAATTTCTAGATCTATTCTCAGTTGCCCTTTTTGAATGGTATACCTTGTTAATTCATATCCATAAGTAAGTGCTAAGCATCTCATAAGACAATTTTTTTTTTTTAATTCCATTTTAAGATATCTTGCGCTAATGGTTTATAAACTGAAATGGAAGTGCTAAGATATAGTTCAGTTCTAATAGTGTATGCCCAGGTGCACATTTTCTGGTCTCACAGAATGACATCATCACCATCTTCTTCTTCTTAATGGTTTAACACATAGAATAGTAACGTGAATTCTCTTCAATTGAATGCCTTTGTTTGAAGTTTTCATGTGAAGTATTTGTCTTTTCCTATAAAGAATGTAGCATAATTGTGATTTCCTACCAAAAATATAACATAATTGTGTTTATTTAAAGTTTTTTTTTTTTGCTATATGGATTCTGTTTTTGCCTGGCTTGCAGGTCTGATTCAGTTTAAATGTGTATTTTGAAGCCAGGAACATTTTATGCTTTGCCTCAAAGTCCACAGCTGTTTAAGCAAATGTTGATGGTGTCTGGTTTTGATAAATATTATCAAGTAGCAAGGTACTGACTATTATATCATTAAGCACTACTTCCATTATGTTTTGTCTAGTTTTGACCATCTGACTTGCATCTACTATTATATTATCTCAATCTTCCCACATTTCCCAGTAAATTCGTTTTATGTTTATGCATTCTCTTTCTTCACAACCGAGATGGTATGTATAGAAAGTTCGTGCATAGATCTAGGTTGATAGATAGTTTCGTTGTTCTTATAAAAATTGGGAGCTAAGTTCAATATGTTTTCAACCCTTTTGAGTTGTGAGAATCGGGTGGTTGTTCTAAGTATGTAACATAAATTGTATTTGCACCGTCAGTTTCATTGTGGATTTCTTTGTAGGTGTTTCCGAGATGAAGACCTAAGAGCGGACAGACAACCTGAGTTTACACAACTTGATATGGAGTTAGCATTTACAACTTTTGAAGACATGCTGAAACTCAATGAAGATTTGATTAGAAGGGTAAACTCTGACTATTAGAATTGACTTGAAGATTGAGTTTCAGAATACATAAGCATAGGTTGTAGTGTAAAAATATTTGAAAAGAAACAAAGATAATTGTCACGATCTTCTGTTCTCACCACCAATTTTTTGCCACGCTTTTTTTGTTGGCACCTATTTTATGCTTGTGTGCATCGTTACCTTTTTGTAATTCGGGTAAAATAAACGTTGAAGGTCATTAAATTTATGTGATTTATGTTGGAGGGATCCAATTTTGTTACCACCTTGTCAACTTTGTTGACACTCATCAGGTTCTTTTCATTAACTTATGTCAATATGAGGAAGTAACTCCATTCAAGAGGGGGATGGACCAATGGATGTTCTAAAGGTCCAAGGCCTAAAAGTACCACTTTTAGTTATATGTTATTATTTGCCCTGCTATTTAATGGCTACTGATGGTAAAGAAAAATTATACTCATAGGTTTTTTGGGAAGTCAAAGGTGTTCAGCTGCCAAATCCTTTCCCGAGGCTTACCTATGCTGAAGCAATGAGTCGATATGGGTCAGATAAGCCTGATACAAGTTTTGATCTTCAATTGAAAGATGTAACTCCTCTTTATACTTCTGGGCCTCATTCTGACTCATGTTTTCGAATGAACTTGTTTTTTATTATGTATTTCCTTAACTAATGAGTGTGCATTGGAAGAAGGAGCCTTCTGGCTTTTGTTGTTCCTTAAAGCAGTTGTTTATTGATTTAAATTATCGCATTACTTTAGAGAATATTCAGTACTTTTTTCATTGTTCGTGCATGTCTGGATGGCCTCAGGATTCATGCTTAACATCCTTATTCAATCATTTTAATTGAATTGGCCTTTTCATAATATGGCTTTCCGGATACCAGTTCTAGGCATTTTAGCAGATTTGATGGGAAAGATTTTCTGGAATATGGTTGGTTGTTGATAAAAAAAAGGATTCTGGAATATGGTCAACTACTTATATGTTAAAGGATTTTGAAACTTATGGATTTATCATCTCAAATTATAGTCAACCATTTTTAGTTTTCAAAGCCCCATTTCCAATTCTACATTCGGCAATAGTATTGTTCACAGGAATTGTGTAATTTGTATGAATTAAAGATTCCTCTAAATTAGCTACTGAGAGATTTTTCTCATTGGAACTGCAGGTATCTGATATTTTTGCAGATTCTCCATTCAAGGTTTTTGCAGACGCCTTGTCTAGTGGTGGAATCATCAAAGTGCTGTGTGTGCCCTCTGGTGCTAAAAGGTATTCAAACACATCTCTGAAAAAGGGTGACATTTATTCAGAGGCAATCAAATCTGGAGCTAAAGGATTACCTTTTCTGAAGGTTCTTGATGATGGTAATCTTCTTTTTCTCTAA

mRNA sequence

TCCACTCGGCCGTCTTCATCCACACGCAGAGTCTTCTTGTTCACCTTTCCTCTGCGAGCGCTCCGTTTCCAGAATCCTTAAACCCTTCCGCCTAACTTCGCCGCTTCCATCAATCTTCCATTGATCCACCTTCAAACCTTCCGCCATGTCCCGCTCTATTCTCCGAGCTCTCCCTTTCTCCACCCTTCGCCACAAGCCTCCTCAATTTCTCCCCATCTTCCACCGTATTTCTCATCTCCGCCTCGCGAAATCCATTTCCAGAACTCTGAGACCTATTTCCTCACTCTCTGTTTCCGCCACTCATCCGCCTTCCTCCGCCACCGAAACGCTCGTTTCCAGTTCGAAGTCTCCGTCGCCTCTTAAGGATTCTCTTCAGTGGATCAATCGTACTGCGTTCTGTGGTGAATTGTCGGCTGGCGATGTTGGGAAGCGGGTTCGGCTTTGTGGTTGGGTTGCACTACATAGGGTTCATGGTGGTTTGACTTTTCTCAATCTTAGAGACCACAGTGGTATCGTACAGGTGACTACGCTACCTAACGAGTTTCCTGATGCTCATTCGGTTATTAACGACTTGCGGCTCGAGTATGTTGTTGCTATAGAAGGCATTGTACGGTCTCGACCTGATGATTCGATTAATGTGAAAATGAAAACTGGCTTGATAGAGGTTGCTGCAGAGCATGTTCAAATCTTAAATTCTGTTGGATCAAAGTTACCTTTTCTAGTTACAATTGCTGATGATGCAAAGGATTCTGCTAAAGAGGAAATTCGATTGAGGTACCGATATCTTGATCTTCGGAGGAAGCAAATGAATTCGAACATAATGCTTCGGCATAAAGTGGTGAAACTCATTCGAAGATATTTGGAGGATGCACATGACTTTGTGGAGATTGAGACGCCAATACTTTCTAGATCTACTCCAGAAGGTGCTCGAGATTATTTGGTGCCGTCTAGAGTTCAGCCAGGAACATTTTATGCTTTGCCTCAAAGTCCACAGCTGTTTAAGCAAATGTTGATGGTGTCTGGTTTTGATAAATATTATCAAGTAGCAAGGTGTTTCCGAGATGAAGACCTAAGAGCGGACAGACAACCTGAGTTTACACAACTTGATATGGAGTTAGCATTTACAACTTTTGAAGACATGCTGAAACTCAATGAAGATTTGATTAGAAGGGTTTTTTGGGAAGTCAAAGGTGTTCAGCTGCCAAATCCTTTCCCGAGGCTTACCTATGCTGAAGCAATGAGTCGATATGGGTCAGATAAGCCTGATACAAGTTTTGATCTTCAATTGAAAGATGTATCTGATATTTTTGCAGATTCTCCATTCAAGGTTTTTGCAGACGCCTTGTCTAGTGGTGGAATCATCAAAGTGCTGTGTGTGCCCTCTGGTGCTAAAAGGTATTCAAACACATCTCTGAAAAAGGGTGACATTTATTCAGAGGCAATCAAATCTGGAGCTAAAGGATTACCTTTTCTGAAGGTTCTTGATGATGGTAATCTTCTTTTTCTCTAA

Coding sequence (CDS)

ATGTCCCGCTCTATTCTCCGAGCTCTCCCTTTCTCCACCCTTCGCCACAAGCCTCCTCAATTTCTCCCCATCTTCCACCGTATTTCTCATCTCCGCCTCGCGAAATCCATTTCCAGAACTCTGAGACCTATTTCCTCACTCTCTGTTTCCGCCACTCATCCGCCTTCCTCCGCCACCGAAACGCTCGTTTCCAGTTCGAAGTCTCCGTCGCCTCTTAAGGATTCTCTTCAGTGGATCAATCGTACTGCGTTCTGTGGTGAATTGTCGGCTGGCGATGTTGGGAAGCGGGTTCGGCTTTGTGGTTGGGTTGCACTACATAGGGTTCATGGTGGTTTGACTTTTCTCAATCTTAGAGACCACAGTGGTATCGTACAGGTGACTACGCTACCTAACGAGTTTCCTGATGCTCATTCGGTTATTAACGACTTGCGGCTCGAGTATGTTGTTGCTATAGAAGGCATTGTACGGTCTCGACCTGATGATTCGATTAATGTGAAAATGAAAACTGGCTTGATAGAGGTTGCTGCAGAGCATGTTCAAATCTTAAATTCTGTTGGATCAAAGTTACCTTTTCTAGTTACAATTGCTGATGATGCAAAGGATTCTGCTAAAGAGGAAATTCGATTGAGGTACCGATATCTTGATCTTCGGAGGAAGCAAATGAATTCGAACATAATGCTTCGGCATAAAGTGGTGAAACTCATTCGAAGATATTTGGAGGATGCACATGACTTTGTGGAGATTGAGACGCCAATACTTTCTAGATCTACTCCAGAAGGTGCTCGAGATTATTTGGTGCCGTCTAGAGTTCAGCCAGGAACATTTTATGCTTTGCCTCAAAGTCCACAGCTGTTTAAGCAAATGTTGATGGTGTCTGGTTTTGATAAATATTATCAAGTAGCAAGGTGTTTCCGAGATGAAGACCTAAGAGCGGACAGACAACCTGAGTTTACACAACTTGATATGGAGTTAGCATTTACAACTTTTGAAGACATGCTGAAACTCAATGAAGATTTGATTAGAAGGGTTTTTTGGGAAGTCAAAGGTGTTCAGCTGCCAAATCCTTTCCCGAGGCTTACCTATGCTGAAGCAATGAGTCGATATGGGTCAGATAAGCCTGATACAAGTTTTGATCTTCAATTGAAAGATGTATCTGATATTTTTGCAGATTCTCCATTCAAGGTTTTTGCAGACGCCTTGTCTAGTGGTGGAATCATCAAAGTGCTGTGTGTGCCCTCTGGTGCTAAAAGGTATTCAAACACATCTCTGAAAAAGGGTGACATTTATTCAGAGGCAATCAAATCTGGAGCTAAAGGATTACCTTTTCTGAAGGTTCTTGATGATGGTAATCTTCTTTTTCTCTAA

Protein sequence

MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATETLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLKKGDIYSEAIKSGAKGLPFLKVLDDGNLLFL

Homology

[Close]

BLAST of Carg08101 vs. NCBI nr
Match: KAG7030925.1 (Aspartate--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 892.9 bits (2306), Expect = 1.2e-255
Identity = 454/454 (100.00%), Postives = 454/454 (100.00%), Query Frame = 0


Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE
Sbjct: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV
Sbjct: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDDGNLLFL 455
           TSLKKGDIYSEAIKSGAKGLPFLKVLDDGNLLFL
Sbjct: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDDGNLLFL 454

[Close]

BLAST of Carg08101 vs. NCBI nr
Match: KAG6600266.1 (Aspartate--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 881.3 bits (2276), Expect = 3.5e-252
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0


Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE
Sbjct: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV
Sbjct: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDD 449
           TSLKKGDIYSEAIKSGAKGLPFLKVLDD
Sbjct: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDD 448

[Close]

BLAST of Carg08101 vs. NCBI nr
Match: XP_022942888.1 (aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita moschata] >XP_022942889.1 aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 876.7 bits (2264), Expect = 8.6e-251
Identity = 445/448 (99.33%), Postives = 447/448 (99.78%), Query Frame = 0


Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRSILRALPFS+LRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE
Sbjct: 1   MSRSILRALPFSSLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLVSSS SPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVSSSNSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV
Sbjct: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDD 449
           T+LKKGDIYSEAIKSGAKGLPFLKVLDD
Sbjct: 421 TALKKGDIYSEAIKSGAKGLPFLKVLDD 448

[Close]

BLAST of Carg08101 vs. NCBI nr
Match: XP_023554090.1 (aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 873.2 bits (2255), Expect = 9.5e-250
Identity = 444/448 (99.11%), Postives = 445/448 (99.33%), Query Frame = 0


Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRSILRALPFSTLRHKPPQFL IFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE
Sbjct: 1   MSRSILRALPFSTLRHKPPQFLSIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLVSSS SPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVSSSNSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV
Sbjct: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDKPDT FDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDKPDTRFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDD 449
           T+LKKGDIYSEAIKSGAKGLPFLKVLDD
Sbjct: 421 TALKKGDIYSEAIKSGAKGLPFLKVLDD 448

[Close]

BLAST of Carg08101 vs. NCBI nr
Match: XP_022975696.1 (aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita maxima] >XP_022975707.1 aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 867.5 bits (2240), Expect = 5.2e-248
Identity = 441/448 (98.44%), Postives = 443/448 (98.88%), Query Frame = 0


Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRSILRALPFS LRHKPPQFLPIF RISHLRLAKSISRTLRPISSLSVSATHPPSSATE
Sbjct: 1   MSRSILRALPFSNLRHKPPQFLPIFRRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLVSSS SPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVSSSNSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRP DSINVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPGDSINVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV
Sbjct: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YA+AMSRYGSDKPDT FDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YADAMSRYGSDKPDTRFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDD 449
           T+LKKGDIYSEAIKSGAKGLPFLKVLDD
Sbjct: 421 TALKKGDIYSEAIKSGAKGLPFLKVLDD 448

[Close]

BLAST of Carg08101 vs. ExPASy Swiss-Prot
Match: F4JJT9 (Aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g33760 PE=2 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 3.3e-176
Identity = 313/448 (69.87%), Postives = 366/448 (81.70%), Query Frame = 0


Query: 5   ILRALPFSTLRHKPPQFLPIFH-RISHLRLAKSISRTLRPISSLSVSATHPPSSATETLV 64
           +LR LP      +P +FL      IS+      + +   P+     + +  P +A    V
Sbjct: 4   LLRTLPL-----RPTRFLSATAISISNATNFFVVPKRTNPLPGTRRTFSSSPVAAASGDV 63

Query: 65  SSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGI 124
                PSP    L+W++RT  CGELS  DVGKRV LCGWVALHRVHGGLTFLNLRDH+GI
Sbjct: 64  VVKPVPSP-PSVLRWVSRTELCGELSVNDVGKRVHLCGWVALHRVHGGLTFLNLRDHTGI 123

Query: 125 VQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILN 184
           VQV TLP+EFP+AH +IND+RLEYVV +EG VRSRP++S+N KMKTG +EV AEHV+ILN
Sbjct: 124 VQVRTLPDEFPEAHGLINDMRLEYVVLVEGTVRSRPNESVNKKMKTGFVEVVAEHVEILN 183

Query: 185 SVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAH 244
            V +KLPFLVT AD+ KD  KEEIRLR+R LDLRR+QM +NI+LRH VVKLIRRYLED H
Sbjct: 184 PVRTKLPFLVTTADENKDLIKEEIRLRFRCLDLRRQQMKNNIVLRHNVVKLIRRYLEDRH 243

Query: 245 DFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARC 304
            F+EIETPILSRSTPEGARDYLVPSR+Q GTFYALPQSPQLFKQMLMVSGFDKYYQ+ARC
Sbjct: 244 GFIEIETPILSRSTPEGARDYLVPSRIQSGTFYALPQSPQLFKQMLMVSGFDKYYQIARC 303

Query: 305 FRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAE 364
           FRDEDLRADRQPEFTQLDME+AF   EDMLKLNEDLIR+VF E+KG+QLP+PFPRLTYA+
Sbjct: 304 FRDEDLRADRQPEFTQLDMEMAFMPMEDMLKLNEDLIRKVFSEIKGIQLPDPFPRLTYAD 363

Query: 365 AMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSL 424
           AM RYGSD+PDT FDL+LKDVS++F +S F+VF +AL SGGIIKVLCVP GAK+YSN++L
Sbjct: 364 AMDRYGSDRPDTRFDLELKDVSNVFTESSFRVFTEALESGGIIKVLCVPLGAKKYSNSAL 423

Query: 425 KKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           KKGDIY+EA+KSGAKGLPFLKVLD+G +
Sbjct: 424 KKGDIYNEAMKSGAKGLPFLKVLDNGEI 445

[Close]

BLAST of Carg08101 vs. ExPASy Swiss-Prot
Match: Q8YUB6 (Aspartate--tRNA(Asp/Asn) ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=aspS PE=3 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 4.8e-127
Identity = 219/372 (58.87%), Postives = 292/372 (78.49%), Query Frame = 0


Query: 81  RTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSVI 140
           RT +CGEL   D+G+ V L GWV   R HGG+ FL+LRD SGIVQV + P   PD++ + 
Sbjct: 2   RTHYCGELRQKDIGETVTLYGWVDRRRDHGGVIFLDLRDRSGIVQVVSDPQRTPDSYELA 61

Query: 141 NDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLVTIADDAK 200
           N LR EYVV I G V  RP++S+N ++ TG +E+ A+ +++LN V  +LPF V+ AD   
Sbjct: 62  NSLRNEYVVEITGRVTQRPEESLNTRIPTGEVEIYADKIELLNGVRKQLPFQVSTAD--T 121

Query: 201 DSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEG 260
           ++ +E++RL+YRYLDLRR +M  NI LRH+VVK +RRYLED   F+E+ETPIL+RSTPEG
Sbjct: 122 ETVREDLRLKYRYLDLRRDRMARNIQLRHQVVKAMRRYLEDVEGFIEVETPILTRSTPEG 181

Query: 261 ARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQL 320
           ARDY++PSRV PG ++ALPQSPQLFKQ+LMVSG D+YYQ+ARCFRDEDLRADRQPEFTQL
Sbjct: 182 ARDYVLPSRVNPGEWFALPQSPQLFKQILMVSGLDRYYQIARCFRDEDLRADRQPEFTQL 241

Query: 321 DMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEAMSRYGSDKPDTSFDLQ 380
           DME++F + E++++LNE L+  +F  VKGV+LP PFPRLTYAEAM RYG DKPDT +DLQ
Sbjct: 242 DMEMSFMSEEEIIELNEKLVSYIFKTVKGVELPLPFPRLTYAEAMERYGCDKPDTRYDLQ 301

Query: 381 LKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLKK-GDIYSEAIKSGAKG 440
           L +VSD+  DS FKVF DA+++GGI+K+L +P+G ++ SN  +K  GD++ EA ++GAKG
Sbjct: 302 LVNVSDVMKDSGFKVFRDAVANGGIVKILPIPNGNEQISNVRIKPGGDLFREASEAGAKG 361

Query: 441 LPFLKVLDDGNL 452
           L +++V +DG +
Sbjct: 362 LAYIRVREDGEI 371

[Close]

BLAST of Carg08101 vs. ExPASy Swiss-Prot
Match: Q3MGL3 (Aspartate--tRNA(Asp/Asn) ligase OS=Trichormus variabilis (strain ATCC 29413 / PCC 7937) OX=240292 GN=aspS PE=3 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 8.1e-127
Identity = 219/372 (58.87%), Postives = 292/372 (78.49%), Query Frame = 0


Query: 81  RTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSVI 140
           RT +CGEL   D+G+ V L GWV   R HGG+ FL+LRD SGIVQV + P   PD++ + 
Sbjct: 21  RTHYCGELRQKDIGETVTLYGWVDRRRDHGGVIFLDLRDRSGIVQVVSDPQRTPDSYELA 80

Query: 141 NDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLVTIADDAK 200
           N LR EYVV I G V  RP++S+N ++ TG +E+ A+ +++LN V  +LPF V+ AD   
Sbjct: 81  NSLRNEYVVEITGRVTQRPEESLNSRIPTGEVEIYADKIELLNGVRKQLPFQVSTAD--T 140

Query: 201 DSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEG 260
           ++ +E++RL+YRYLDLRR +M  NI LRH+VVK +RRYLED   F+E+ETPIL+RSTPEG
Sbjct: 141 ETVREDLRLKYRYLDLRRDRMARNIQLRHQVVKAMRRYLEDVEGFIEVETPILTRSTPEG 200

Query: 261 ARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQL 320
           ARDY++PSRV PG ++ALPQSPQLFKQ+LMVSG D+YYQ+ARCFRDEDLRADRQPEFTQL
Sbjct: 201 ARDYVLPSRVNPGEWFALPQSPQLFKQILMVSGMDRYYQIARCFRDEDLRADRQPEFTQL 260

Query: 321 DMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEAMSRYGSDKPDTSFDLQ 380
           DME++F + E++++LNE L+  +F  VKGV+LP PFPRLTYAEAM RYG DKPDT +DLQ
Sbjct: 261 DMEMSFMSEEEIIQLNEKLVSYIFKTVKGVELPLPFPRLTYAEAMERYGCDKPDTRYDLQ 320

Query: 381 LKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLKK-GDIYSEAIKSGAKG 440
           L +VSD+  DS FKVF DA+++GGI+K+L +P+G ++ SN  +K  GD++ EA ++GAKG
Sbjct: 321 LVNVSDVMKDSGFKVFRDAVANGGIVKILPIPNGNEQISNVRIKPGGDLFREASEAGAKG 380

Query: 441 LPFLKVLDDGNL 452
           L +++V +DG +
Sbjct: 381 LAYIRVREDGEI 390

[Close]

BLAST of Carg08101 vs. ExPASy Swiss-Prot
Match: Q112K0 (Aspartate--tRNA(Asp/Asn) ligase OS=Trichodesmium erythraeum (strain IMS101) OX=203124 GN=aspS PE=3 SV=1)

HSP 1 Score: 454.9 bits (1169), Expect = 1.1e-126
Identity = 216/372 (58.06%), Postives = 291/372 (78.23%), Query Frame = 0


Query: 81  RTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSVI 140
           RT +CG++++ ++ + V LCGWV   R HGG+ FL+LRD SG++Q+ + P   PD++ + 
Sbjct: 2   RTHYCGQVNSNNIEETVNLCGWVDRRRDHGGVIFLDLRDRSGLIQIVSDPERTPDSYQIA 61

Query: 141 NDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLVTIADDAK 200
            D+R EYVV I G V  RP++S+N K+ TG IE+ A+ +Q+LN +G +LPF V+ A+   
Sbjct: 62  GDIRNEYVVQITGRVSRRPEESLNPKLPTGEIEIYADDIQLLNGLGKQLPFQVSTAE--T 121

Query: 201 DSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEG 260
           ++ +EE+RL+YRYLDLRR++M  N++LRH+V+K IRR+LED  +FVEIETPIL+RSTPEG
Sbjct: 122 EAVREELRLKYRYLDLRRERMTRNLLLRHEVIKAIRRFLEDEQNFVEIETPILTRSTPEG 181

Query: 261 ARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQL 320
           ARDYLVPSRV PG ++ALPQSPQLFKQ+LMVSGFD+YYQ+ARCFRDEDLRADRQPEFTQL
Sbjct: 182 ARDYLVPSRVHPGEWFALPQSPQLFKQILMVSGFDRYYQIARCFRDEDLRADRQPEFTQL 241

Query: 321 DMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEAMSRYGSDKPDTSFDLQ 380
           DME++F + E++L+LNE L+  +F +VKG+ LP PFPRLTY EAM RYGSDKPD  F L+
Sbjct: 242 DMEMSFMSQEEILQLNEKLVAYIFQKVKGIDLPLPFPRLTYTEAMERYGSDKPDVRFGLE 301

Query: 381 LKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLKK-GDIYSEAIKSGAKG 440
           L DVSD+  DS FKVF+ A++ GGI+KVL +P G    SN  +K  GD++ EA + GAKG
Sbjct: 302 LVDVSDLMKDSGFKVFSGAIAKGGIVKVLPIPGGNDAISNVRIKPGGDLFKEASEVGAKG 361

Query: 441 LPFLKVLDDGNL 452
           L +++V + G +
Sbjct: 362 LAYVRVKEGGKI 371

[Close]

BLAST of Carg08101 vs. ExPASy Swiss-Prot
Match: B0CF01 (Aspartate--tRNA(Asp/Asn) ligase OS=Acaryochloris marina (strain MBIC 11017) OX=329726 GN=aspS PE=3 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 5.8e-125
Identity = 214/370 (57.84%), Postives = 288/370 (77.84%), Query Frame = 0


Query: 81  RTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEFPDAHSVI 140
           RT +C EL    VG+RV LCGWV   R HGG+ FL++RD +G++Q+ + P   P+A+   
Sbjct: 2   RTHYCSELRNDHVGERVTLCGWVDRRRDHGGVIFLDVRDRTGLIQIVSDPERTPEAYPQA 61

Query: 141 NDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLVTIADDAK 200
           + LR EYVV I G V  RPDDS+N ++ TG +E+ A+ +++LN+V  +LPF V+ AD   
Sbjct: 62  DALRNEYVVRIHGEVSRRPDDSLNPRLPTGEVEIYADQIELLNAVHQQLPFQVSTAD--V 121

Query: 201 DSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEG 260
           +S KEE+RL+YRYLDLRR +M+ NIM+RH++++ IRRYLED  +FV+IETP+L+RSTPEG
Sbjct: 122 ESVKEELRLKYRYLDLRRDRMHQNIMMRHRLIQSIRRYLEDQQNFVDIETPVLTRSTPEG 181

Query: 261 ARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQL 320
           ARDYLVPSR  PG ++ALPQSPQ+FKQ+LMVSGFD+YYQ+ARCFRDEDLRADRQPEFTQL
Sbjct: 182 ARDYLVPSRANPGEWFALPQSPQIFKQLLMVSGFDRYYQIARCFRDEDLRADRQPEFTQL 241

Query: 321 DMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAEAMSRYGSDKPDTSFDLQ 380
           DME++F + E++++LNEDL+R VF  +K + LP  FP++TYAEAM R+G+D+PDT FDL+
Sbjct: 242 DMEMSFMSQEEIIQLNEDLMRHVFKTLKDIDLPKTFPQMTYAEAMERFGTDRPDTRFDLE 301

Query: 381 LKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSLKK-GDIYSEAIKSGAKG 440
           L +VSD+  DS FKVF+ A+  GG +KVL +P G  + SN  +K  GD++SEA   GAKG
Sbjct: 302 LVNVSDLLEDSGFKVFSGAIKKGGQVKVLPIPDGNAKISNVRIKPGGDLFSEASAGGAKG 361

Query: 441 LPFLKVLDDG 450
           L +++V DDG
Sbjct: 362 LAYIRVKDDG 369

[Close]

BLAST of Carg08101 vs. ExPASy TrEMBL
Match: A0A6J1FSM9 (aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447784 PE=3 SV=1)

HSP 1 Score: 876.7 bits (2264), Expect = 4.2e-251
Identity = 445/448 (99.33%), Postives = 447/448 (99.78%), Query Frame = 0


Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRSILRALPFS+LRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE
Sbjct: 1   MSRSILRALPFSSLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLVSSS SPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVSSSNSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV
Sbjct: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDD 449
           T+LKKGDIYSEAIKSGAKGLPFLKVLDD
Sbjct: 421 TALKKGDIYSEAIKSGAKGLPFLKVLDD 448

[Close]

BLAST of Carg08101 vs. ExPASy TrEMBL
Match: A0A6J1IDT0 (aspartate--tRNA ligase, chloroplastic/mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111475596 PE=3 SV=1)

HSP 1 Score: 867.5 bits (2240), Expect = 2.5e-248
Identity = 441/448 (98.44%), Postives = 443/448 (98.88%), Query Frame = 0


Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRSILRALPFS LRHKPPQFLPIF RISHLRLAKSISRTLRPISSLSVSATHPPSSATE
Sbjct: 1   MSRSILRALPFSNLRHKPPQFLPIFRRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLVSSS SPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVSSSNSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRP DSINVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPGDSINVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV
Sbjct: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YA+AMSRYGSDKPDT FDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YADAMSRYGSDKPDTRFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDD 449
           T+LKKGDIYSEAIKSGAKGLPFLKVLDD
Sbjct: 421 TALKKGDIYSEAIKSGAKGLPFLKVLDD 448

[Close]

BLAST of Carg08101 vs. ExPASy TrEMBL
Match: A0A5D3E1M9 (Aspartate--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00320 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.0e-228
Identity = 402/451 (89.14%), Postives = 419/451 (92.90%), Query Frame = 0


Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRS+LRA PFS +R KPPQFLPI H ISHLRL +SIS T  P+SS S SAT PPSSATE
Sbjct: 1   MSRSLLRAFPFSNIRPKPPQFLPILHHISHLRLPQSISTTQTPVSSFSTSATPPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLV +S S SP KDSLQWINRTA CGELS  DVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVPTSNSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHS INDLRLEYVVA+EG VRSRP DS+NVKMKTG IEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGFIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVT ADDAKDSAKEEIRLRYRYLDLRRKQMN NIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           D H FVEIETPILSRSTPEGARDYLVPSRVQPGTF+ALPQSPQLFKQMLMVSGFDKYYQ+
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMEL+FTT EDML+LNEDLIR+VFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSD+PDT FDLQL DVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           T+LKKGDIY+EA+KSGAKGLPFLKV DDG +
Sbjct: 421 TALKKGDIYTEAMKSGAKGLPFLKVTDDGGI 451

[Close]

BLAST of Carg08101 vs. ExPASy TrEMBL
Match: A0A1S3BZT5 (aspartate--tRNA ligase, chloroplastic/mitochondrial isoform X1 OS=Cucumis melo OX=3656 GN=LOC103494835 PE=3 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.0e-228
Identity = 402/451 (89.14%), Postives = 419/451 (92.90%), Query Frame = 0


Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRS+LRA PFS +R KPPQFLPI H ISHLRL +SIS T  P+SS S SAT PPSSATE
Sbjct: 1   MSRSLLRAFPFSNIRPKPPQFLPILHHISHLRLPQSISTTQTPVSSFSTSATPPPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
           TLV +S S SP KDSLQWINRTA CGELS  DVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  TLVPTSNSHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHS INDLRLEYVVA+EG VRSRP DS+NVKMKTG IEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSAINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGFIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVT ADDAKDSAKEEIRLRYRYLDLRRKQMN NIMLRHKVVKLIRRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLIRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           D H FVEIETPILSRSTPEGARDYLVPSRVQPGTF+ALPQSPQLFKQMLMVSGFDKYYQ+
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMEL+FTT EDML+LNEDLIR+VFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSD+PDT FDLQL DVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           T+LKKGDIY+EA+KSGAKGLPFLKV DDG +
Sbjct: 421 TALKKGDIYTEAMKSGAKGLPFLKVTDDGGI 451

[Close]

BLAST of Carg08101 vs. ExPASy TrEMBL
Match: A0A0A0KT15 (AA_TRNA_LIGASE_II domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G000540 PE=3 SV=1)

HSP 1 Score: 796.2 bits (2055), Expect = 7.2e-227
Identity = 400/451 (88.69%), Postives = 417/451 (92.46%), Query Frame = 0


Query: 1   MSRSILRALPFSTLRHKPPQFLPIFHRISHLRLAKSISRTLRPISSLSVSATHPPSSATE 60
           MSRS+LRA PFST+R KPPQFLPI H ISHLR  +SIS T RP+SS S SAT  PSSATE
Sbjct: 1   MSRSLLRAFPFSTIRPKPPQFLPIRHHISHLRFPQSISTTHRPVSSFSTSATPSPSSATE 60

Query: 61  TLVSSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120
            LV +S   SP KDSLQWINRTA CGELS  DVGKRVRLCGWVALHRVHGGLTFLNLRDH
Sbjct: 61  ILVPTSNPHSPFKDSLQWINRTALCGELSVDDVGKRVRLCGWVALHRVHGGLTFLNLRDH 120

Query: 121 SGIVQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQ 180
           SGIVQVTTLPNEFPDAHS INDLRLEYVVA+EG VRSRP DS+NVKMKTGLIEVAAEHVQ
Sbjct: 121 SGIVQVTTLPNEFPDAHSTINDLRLEYVVAVEGTVRSRPGDSVNVKMKTGLIEVAAEHVQ 180

Query: 181 ILNSVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLE 240
           ILNSVGSKLPFLVT ADDAKDSAKEEIRLRYRYLDLRRKQMN NIMLRHKVVKL+RRYLE
Sbjct: 181 ILNSVGSKLPFLVTTADDAKDSAKEEIRLRYRYLDLRRKQMNFNIMLRHKVVKLLRRYLE 240

Query: 241 DAHDFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQV 300
           D H FVEIETPILSRSTPEGARDYLVPSRVQPGTF+ALPQSPQLFKQMLMVSGFDKYYQ+
Sbjct: 241 DVHGFVEIETPILSRSTPEGARDYLVPSRVQPGTFFALPQSPQLFKQMLMVSGFDKYYQI 300

Query: 301 ARCFRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLT 360
           ARCFRDEDLRADRQPEFTQLDMEL+FTT EDML+LNEDLIR+VFWEVKGVQLPNPFPRLT
Sbjct: 301 ARCFRDEDLRADRQPEFTQLDMELSFTTLEDMLRLNEDLIRKVFWEVKGVQLPNPFPRLT 360

Query: 361 YAEAMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420
           YAEAMSRYGSD+PDT FDLQL DVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN
Sbjct: 361 YAEAMSRYGSDRPDTRFDLQLNDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSN 420

Query: 421 TSLKKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           T+LKKGDIY+EA KSGAKGLPFLKV DDG +
Sbjct: 421 TALKKGDIYTEATKSGAKGLPFLKVTDDGGI 451

[Close]

BLAST of Carg08101 vs. TAIR 10
Match: AT4G33760.1 (tRNA synthetase class II (D, K and N) family protein )

HSP 1 Score: 619.4 bits (1596), Expect = 2.3e-177
Identity = 313/448 (69.87%), Postives = 366/448 (81.70%), Query Frame = 0


Query: 5   ILRALPFSTLRHKPPQFLPIFH-RISHLRLAKSISRTLRPISSLSVSATHPPSSATETLV 64
           +LR LP      +P +FL      IS+      + +   P+     + +  P +A    V
Sbjct: 4   LLRTLPL-----RPTRFLSATAISISNATNFFVVPKRTNPLPGTRRTFSSSPVAAASGDV 63

Query: 65  SSSKSPSPLKDSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGI 124
                PSP    L+W++RT  CGELS  DVGKRV LCGWVALHRVHGGLTFLNLRDH+GI
Sbjct: 64  VVKPVPSP-PSVLRWVSRTELCGELSVNDVGKRVHLCGWVALHRVHGGLTFLNLRDHTGI 123

Query: 125 VQVTTLPNEFPDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILN 184
           VQV TLP+EFP+AH +IND+RLEYVV +EG VRSRP++S+N KMKTG +EV AEHV+ILN
Sbjct: 124 VQVRTLPDEFPEAHGLINDMRLEYVVLVEGTVRSRPNESVNKKMKTGFVEVVAEHVEILN 183

Query: 185 SVGSKLPFLVTIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRHKVVKLIRRYLEDAH 244
            V +KLPFLVT AD+ KD  KEEIRLR+R LDLRR+QM +NI+LRH VVKLIRRYLED H
Sbjct: 184 PVRTKLPFLVTTADENKDLIKEEIRLRFRCLDLRRQQMKNNIVLRHNVVKLIRRYLEDRH 243

Query: 245 DFVEIETPILSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARC 304
            F+EIETPILSRSTPEGARDYLVPSR+Q GTFYALPQSPQLFKQMLMVSGFDKYYQ+ARC
Sbjct: 244 GFIEIETPILSRSTPEGARDYLVPSRIQSGTFYALPQSPQLFKQMLMVSGFDKYYQIARC 303

Query: 305 FRDEDLRADRQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKGVQLPNPFPRLTYAE 364
           FRDEDLRADRQPEFTQLDME+AF   EDMLKLNEDLIR+VF E+KG+QLP+PFPRLTYA+
Sbjct: 304 FRDEDLRADRQPEFTQLDMEMAFMPMEDMLKLNEDLIRKVFSEIKGIQLPDPFPRLTYAD 363

Query: 365 AMSRYGSDKPDTSFDLQLKDVSDIFADSPFKVFADALSSGGIIKVLCVPSGAKRYSNTSL 424
           AM RYGSD+PDT FDL+LKDVS++F +S F+VF +AL SGGIIKVLCVP GAK+YSN++L
Sbjct: 364 AMDRYGSDRPDTRFDLELKDVSNVFTESSFRVFTEALESGGIIKVLCVPLGAKKYSNSAL 423

Query: 425 KKGDIYSEAIKSGAKGLPFLKVLDDGNL 452
           KKGDIY+EA+KSGAKGLPFLKVLD+G +
Sbjct: 424 KKGDIYNEAMKSGAKGLPFLKVLDNGEI 445

[Close]

BLAST of Carg08101 vs. TAIR 10
Match: AT3G13490.1 (Lysyl-tRNA synthetase, class II )

HSP 1 Score: 75.9 bits (185), Expect = 9.5e-14
Identity = 68/277 (24.55%), Postives = 121/277 (43.68%), Query Frame = 0


Query: 74  DSLQWINRTAFCGELSAGDVGKRVRLCGWVALHRVHGGLTFLNLRDHSGIVQVTTLPNEF 133
           + LQ I +    GE S  ++   V + G V   R  G L FL LRD SG +Q+       
Sbjct: 114 NQLQEIYKHLANGEESDNEI-DCVSIAGRVVARRAFGKLAFLTLRDDSGTIQLYCEKERL 173

Query: 134 PDAHSVINDLRLEYVVAIEGIVRSRPDDSINVKMKTGLIEVAAEHVQILNSVGSKLPFLV 193
            D        +L+  + I  I+ +        + + G + +      IL         L+
Sbjct: 174 SDDQFE----QLKQFIDIGDILGA---SGSMKRTEKGELSICVNSFSILTK------SLL 233

Query: 194 TIADDAKDSAKEEIRLRYRYLDLRRKQMNSNIMLRH-KVVKLIRRYLEDAHDFVEIETPI 253
            + D        + R R RY+D+      +++  R  K+V  IR+ +E +  ++E+ETP+
Sbjct: 234 PLPDKYHGLTDIDKRYRQRYVDMIANPEVADVFRRRAKIVSEIRKTVE-SFGYLEVETPV 293

Query: 254 LSRSTPEGARDYLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRAD 313
           L  +         V      G    L  + +L  + ++V GF+K Y++ R FR+E +   
Sbjct: 294 LQGAAGGAEARPFVTFHNSLGRDLYLRIATELHLKRMLVGGFEKVYEIGRIFRNEGISTR 353

Query: 314 RQPEFTQLDMELAFTTFEDMLKLNEDLIRRVFWEVKG 350
             PEFT ++M  A++ +  M+ + E ++ +    V G
Sbjct: 354 HNPEFTTIEMYEAYSDYHSMMDMAELIVTQCSMAVNG 375

[Close]

BLAST of Carg08101 vs. TAIR 10
Match: AT3G11710.1 (lysyl-tRNA synthetase 1 )

HSP 1 Score: 66.6 bits (161), Expect = 5.8e-11
Identity = 38/146 (26.03%), Postives = 77/146 (52.74%), Query Frame = 0


Query: 205 EEIRLRYRYLD-LRRKQMNSNIMLRHKVVKLIRRYLEDAHDFVEIETPILSRSTPEGARD 264
           +E R R R+LD +   ++      R K++  +RR+L D  +F+E+ETP+++      A  
Sbjct: 259 QESRYRQRHLDMILNVEVRQIFRTRAKIISYVRRFL-DNKNFLEVETPMMNMIAGGAAAR 318

Query: 265 YLVPSRVQPGTFYALPQSPQLFKQMLMVSGFDKYYQVARCFRDEDLRADRQPEFTQLDME 324
             V           +  +P+L+ + L+V G ++ Y++ + FR+E +     PEFT  +  
Sbjct: 319 PFVTHHNDLDMRLYMRIAPELYLKQLIVGGLERVYEIGKQFRNEGIDLTHNPEFTTCEFY 378

Query: 325 LAFTTFEDMLKLNEDLIRRVFWEVKG 350
           +AF  + D++++ E ++  +  E+ G
Sbjct: 379 MAFADYNDLMEMTEVMLSGMVKELTG 403

The following BLAST results are available for this feature:

BLAST of Carg08101 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7030925.1	1.2e-255	100.00	Aspartate--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subs...	[more]
KAG6600266.1	3.5e-252	100.00	Aspartate--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosp...	[more]
XP_022942888.1	8.6e-251	99.33	aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita m...	[more]
XP_023554090.1	9.5e-250	99.11	aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 [Cucurbita p...	[more]
XP_022975696.1	5.2e-248	98.44	aspartate--tRNA ligase, chloroplastic/mitochondrial-like [Cucurbita maxima] >XP_...	[more]

BLAST of Carg08101 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JJT9	3.3e-176	69.87	Aspartate--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3...	[more]
Q8YUB6	4.8e-127	58.87	Aspartate--tRNA(Asp/Asn) ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTE...	[more]
Q3MGL3	8.1e-127	58.87	Aspartate--tRNA(Asp/Asn) ligase OS=Trichormus variabilis (strain ATCC 29413 / PC...	[more]
Q112K0	1.1e-126	58.06	Aspartate--tRNA(Asp/Asn) ligase OS=Trichodesmium erythraeum (strain IMS101) OX=2...	[more]
B0CF01	5.8e-125	57.84	Aspartate--tRNA(Asp/Asn) ligase OS=Acaryochloris marina (strain MBIC 11017) OX=3...	[more]

BLAST of Carg08101 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FSM9	4.2e-251	99.33	aspartate--tRNA ligase, chloroplastic/mitochondrial-like isoform X1 OS=Cucurbita...	[more]
A0A6J1IDT0	2.5e-248	98.44	aspartate--tRNA ligase, chloroplastic/mitochondrial-like OS=Cucurbita maxima OX=...	[more]
A0A5D3E1M9	1.0e-228	89.14	Aspartate--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold6...	[more]
A0A1S3BZT5	1.0e-228	89.14	aspartate--tRNA ligase, chloroplastic/mitochondrial isoform X1 OS=Cucumis melo O...	[more]
A0A0A0KT15	7.2e-227	88.69	AA_TRNA_LIGASE_II domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]

BLAST of Carg08101 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G33760.1	2.3e-177	69.87	tRNA synthetase class II (D, K and N) family protein	[more]
AT3G13490.1	9.5e-14	24.55	Lysyl-tRNA synthetase, class II	[more]
AT3G11710.1	5.8e-11	26.03	lysyl-tRNA synthetase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	PRINTS	PR01042	TRNASYNTHASP	coord: 295..308 score: 49.51 coord: 278..290 score: 65.38
None	No IPR available	GENE3D	2.40.50.140		coord: 73..185 e-value: 1.1E-31 score: 110.9
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 195..391 e-value: 5.0E-63 score: 215.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 47..70
None	No IPR available	PANTHER	PTHR22594	ASPARTYL/LYSYL-TRNA SYNTHETASE	coord: 64..447
None	No IPR available	CDD	cd04317	EcAspRS_like_N	coord: 81..221 e-value: 9.22415E-61 score: 192.736
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 205..398
IPR029351	GAD domain	PFAM	PF02938	GAD	coord: 396..449 e-value: 2.2E-9 score: 37.5
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 205..380 e-value: 2.2E-64 score: 217.5
IPR004524	Aspartate-tRNA ligase, type 1	TIGRFAM	TIGR00459	TIGR00459	coord: 80..449 e-value: 2.9E-141 score: 469.7
IPR004524	Aspartate-tRNA ligase, type 1	PANTHER	PTHR22594:SF5	ASPARTATE--TRNA LIGASE, MITOCHONDRIAL	coord: 64..447
IPR004365	OB-fold nucleic acid binding domain, AA-tRNA synthetase-type	PFAM	PF01336	tRNA_anti-codon	coord: 97..180 e-value: 1.0E-11 score: 44.6
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 227..381 score: 13.525171
IPR004115	GAD-like domain superfamily	SUPERFAMILY	55261	GAD domain-like	coord: 377..449
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 81..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg08101-RA	Carg08101-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006422	aspartyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005739	mitochondrion
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004815	aspartate-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0000166	nucleotide binding