Carg06757 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg06757
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	High-affinity nitrate transporter
Location	Carg_Chr18: 1045202 .. 1045904 (+)
RNA-Seq Expression	Carg06757
Synteny	Carg06757

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTACTCATTTACGCGGTCTCCTATGCCTGCACTGACCTGTTCTCTAGTCTCTTCTGTATCACTGCTTCTCCAAAATCTGGACAAGGTAAGAACTTTCAGCTCCCATAACTCAAATTTCTGATGCTTCCAACACAGATCTGAATCAAAAACTGTTAGTTTAGCGATTTTTAACATCTGGGTTTTTGTTTTTTTTGCAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGTCATGGGTTCTTAATGGAGCTGCAAAAGTTGGGTCGGATTTGGCTTACAAATCCGTGAAGGTGAAGCTCTGCTATGCGCCGGTGAGCCAAGTGGACCGTGCGTGGAGGAAAACAGAGGACGATCTTGCAAAGGACAAGACTTGTCAATTCCAAATCGTGGAGAAGCCGTACAATCCGTCGAACAAGACGGTTCAAAAGGCTGAGTGGACGGTGGAGCGTGACACTCCGACGGCGACTTTCTTCGTGCGTGCTTACGCGTTCAATTCCGCCGGCGATGAAGTGGCGTACGGACAAACGACAGACGAAAAGAAAGGCACAAATTTGTTCCAAGTTGAAGCTATCACTGGCCGCCATATCTCTCTCGATATTGCTTCTGGTTGTTTCAGCGCTTTCTCTGTGCTGTCTTTGACCGGATTCTTCATCGCCGGTAAGAGGCAGGCGAAGAAGGCGGCGGCGGCGGAGGGGCACTAA

mRNA sequence

ATGGTACTCATTTACGCGGTCTCCTATGCCTGCACTGACCTGTTCTCTAGTCTCTTCTGTATCACTGCTTCTCCAAAATCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGTCATGGGTTCTTAATGGAGCTGCAAAAGTTGGGTCGGATTTGGCTTACAAATCCGTGAAGGTGAAGCTCTGCTATGCGCCGGTGAGCCAAGTGGACCGTGCGTGGAGGAAAACAGAGGACGATCTTGCAAAGGACAAGACTTGTCAATTCCAAATCGTGGAGAAGCCGTACAATCCGTCGAACAAGACGGTTCAAAAGGCTGAGTGGACGGTGGAGCGTGACACTCCGACGGCGACTTTCTTCGTGCGTGCTTACGCGTTCAATTCCGCCGGCGATGAAGTGGCGTACGGACAAACGACAGACGAAAAGAAAGGCACAAATTTGTTCCAAGTTGAAGCTATCACTGGCCGCCATATCTCTCTCGATATTGCTTCTGGTTGTTTCAGCGCTTTCTCTGTGCTGTCTTTGACCGGATTCTTCATCGCCGGTAAGAGGCAGGCGAAGAAGGCGGCGGCGGCGGAGGGGCACTAA

Coding sequence (CDS)

ATGGTACTCATTTACGCGGTCTCCTATGCCTGCACTGACCTGTTCTCTAGTCTCTTCTGTATCACTGCTTCTCCAAAATCTGGACAAGTTCTCAAAGCGGGTGTTGATAAAATCAGTGTGTCATGGGTTCTTAATGGAGCTGCAAAAGTTGGGTCGGATTTGGCTTACAAATCCGTGAAGGTGAAGCTCTGCTATGCGCCGGTGAGCCAAGTGGACCGTGCGTGGAGGAAAACAGAGGACGATCTTGCAAAGGACAAGACTTGTCAATTCCAAATCGTGGAGAAGCCGTACAATCCGTCGAACAAGACGGTTCAAAAGGCTGAGTGGACGGTGGAGCGTGACACTCCGACGGCGACTTTCTTCGTGCGTGCTTACGCGTTCAATTCCGCCGGCGATGAAGTGGCGTACGGACAAACGACAGACGAAAAGAAAGGCACAAATTTGTTCCAAGTTGAAGCTATCACTGGCCGCCATATCTCTCTCGATATTGCTTCTGGTTGTTTCAGCGCTTTCTCTGTGCTGTCTTTGACCGGATTCTTCATCGCCGGTAAGAGGCAGGCGAAGAAGGCGGCGGCGGCGGAGGGGCACTAA

Protein sequence

MVLIYAVSYACTDLFSSLFCITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH

Homology

[Close]

BLAST of Carg06757 vs. NCBI nr
Match: KAG7012276.1 (High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 386.3 bits (991), Expect = 1.5e-103
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 0


Query: 1   MVLIYAVSYACTDLFSSLFCITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVK 60
           MVLIYAVSYACTDLFSSLFCITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVK
Sbjct: 1   MVLIYAVSYACTDLFSSLFCITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVK 60

Query: 61  VKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATF 120
           VKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATF
Sbjct: 61  VKLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATF 120

Query: 121 FVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFF 180
           FVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFF
Sbjct: 121 FVRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFF 180

Query: 181 IAGKRQAKKAAAAEGH 197
           IAGKRQAKKAAAAEGH
Sbjct: 181 IAGKRQAKKAAAAEGH 196

[Close]

BLAST of Carg06757 vs. NCBI nr
Match: XP_022954957.1 (high-affinity nitrate transporter 3.1-like [Cucurbita moschata] >KAG6573089.1 High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 348.6 bits (893), Expect = 3.5e-92
Identity = 175/176 (99.43%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 21  ITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 80
           +TASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED
Sbjct: 35  VTASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 94

Query: 81  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 140
           DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT
Sbjct: 95  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 154

Query: 141 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 197
           DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH
Sbjct: 155 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210

[Close]

BLAST of Carg06757 vs. NCBI nr
Match: XP_023542774.1 (high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 346.3 bits (887), Expect = 1.8e-91
Identity = 172/176 (97.73%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 21  ITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 80
           +TASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED
Sbjct: 35  VTASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 94

Query: 81  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 140
           DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT
Sbjct: 95  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 154

Query: 141 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 197
           DEKKGTNLFQ+EAI+GRH+SLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH
Sbjct: 155 DEKKGTNLFQIEAISGRHVSLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210

[Close]

BLAST of Carg06757 vs. NCBI nr
Match: XP_022994709.1 (high-affinity nitrate transporter 3.1-like [Cucurbita maxima])

HSP 1 Score: 339.7 bits (870), Expect = 1.6e-89
Identity = 170/176 (96.59%), Postives = 174/176 (98.86%), Query Frame = 0


Query: 21  ITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 80
           +TASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED
Sbjct: 35  VTASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 94

Query: 81  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 140
           DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTV+RDTPTATFFVRAYAFNSAGDEVAYGQTT
Sbjct: 95  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVKRDTPTATFFVRAYAFNSAGDEVAYGQTT 154

Query: 141 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 197
           DEKKGTNLFQVEAI+GRH SLDIAS CFSAFSVLSLTGFFIAGKR+AKKAAAAEGH
Sbjct: 155 DEKKGTNLFQVEAISGRHASLDIASACFSAFSVLSLTGFFIAGKRKAKKAAAAEGH 210

[Close]

BLAST of Carg06757 vs. NCBI nr
Match: XP_022924070.1 (high-affinity nitrate transporter 3.1 [Cucurbita moschata])

HSP 1 Score: 293.9 bits (751), Expect = 1.0e-75
Identity = 145/172 (84.30%), Postives = 155/172 (90.12%), Query Frame = 0


Query: 21  ITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 80
           +TASPK GQVLKAGVDKISV+WVLNG  K GSD +YK+V+ KLCYAPVSQVDRAWRKTED
Sbjct: 41  VTASPKPGQVLKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTED 100

Query: 81  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 140
           DL+KDKTCQF IV KPYNP NKTVQK EWTV RD PT TFFVRAYAFNSAGDEV YGQTT
Sbjct: 101 DLSKDKTCQFLIVGKPYNPGNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTT 160

Query: 141 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAA 193
           D KKGTNLFQ+EAITGRHISLDIAS CFSAFSVLSL+GFF+AGKR+AKKA A
Sbjct: 161 DNKKGTNLFQIEAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEA 212

[Close]

BLAST of Carg06757 vs. ExPASy Swiss-Prot
Match: Q9FGS5 (High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 PE=1 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 4.0e-46
Identity = 95/163 (58.28%), Postives = 121/163 (74.23%), Query Frame = 0


Query: 28  GQVLKAGVDKISVSWVLNGAAKVGS--DLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKD 87
           G VL AG D ++++W L   + +GS  +  +K +KVKLCYAP SQVDR WRKT D+L KD
Sbjct: 49  GVVLDAGKDTLNITWTL---SSIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKD 108

Query: 88  KTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKG 147
           KTC  +I+ KPY   +KT+Q   WT+ERD PT T+FVRAYA ++ G EVAYGQ+TD+ K 
Sbjct: 109 KTCPHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKK 168

Query: 148 TNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 189
           TNLF V+AI+GRH SLDIAS CFS FSV++L  FF+  KR+AK
Sbjct: 169 TNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Carg06757 vs. ExPASy Swiss-Prot
Match: Q9SB67 (High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 PE=2 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 9.8e-45
Identity = 96/179 (53.63%), Postives = 129/179 (72.07%), Query Frame = 0


Query: 12  TDLFSSLFCITASP-KSGQVLKAGVDKISVSWVL-NGAAKVGSDLAYKSVKVKLCYAPVS 71
           TDL +S+  +TA P K   VL+AG D ++++W L + +AKV +D A+K+++VKLCYAP+S
Sbjct: 30  TDLQNSIE-VTAKPVKDSGVLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPIS 89

Query: 72  QVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNS 131
           QVDR WRKT++ L KD++C  +IV K Y   +KT Q  +WT+  D PT T+FVRAY  + 
Sbjct: 90  QVDRPWRKTDNKLFKDRSCPHEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRAYGIDG 149

Query: 132 AGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 189
            G EVAYGQ+TDE + TNLF V AI+G H+ LDIAS  FS FSV+SL  FF+  KR+AK
Sbjct: 150 DGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 204

[Close]

BLAST of Carg06757 vs. ExPASy Swiss-Prot
Match: Q6ZI50 (High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.1 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 1.8e-38
Identity = 86/188 (45.74%), Postives = 119/188 (63.30%), Query Frame = 0


Query: 6   AVSYACTDLFSS----LFCITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKV 65
           A + A T +F S       +  SPK G+V+ AG + ++V+W LN +   G+D A+KSVKV
Sbjct: 26  AAAAAKTQVFLSKLPKALVVGVSPKHGEVVHAGENTVTVTWSLNTSEPAGADAAFKSVKV 85

Query: 66  KLCYAPVSQVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFF 125
           KLCYAP S+ DR WRK  DDL KDK CQF++  +PY        + ++ V RD PTA++F
Sbjct: 86  KLCYAPASRTDRGWRKASDDLHKDKACQFKVTVQPYAAG---AGRFDYVVARDIPTASYF 145

Query: 126 VRAYAFNSAGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFI 185
           VRAYA +++G EVAYGQ++ +      F V  ITG H SL +A+G FS FS+ +L  FF+
Sbjct: 146 VRAYAVDASGTEVAYGQSSPDA----AFDVAGITGIHASLKVAAGVFSTFSIAALAFFFV 205

Query: 186 AGKRQAKK 190
             KR+  K
Sbjct: 206 VEKRKKDK 206

[Close]

BLAST of Carg06757 vs. ExPASy Swiss-Prot
Match: Q7XK12 (Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NAR2.2 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 5.2e-38
Identity = 84/172 (48.84%), Postives = 113/172 (65.70%), Query Frame = 0


Query: 21  ITASPKSGQVLKAGVDKISVSWVLN---GAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRK 80
           + ASP++GQVL AG D I+V+W LN    AA  G+D  YK+VKV LCYAP SQV R WRK
Sbjct: 47  VEASPRAGQVLHAGEDAITVTWSLNATAAAAAAGADAGYKAVKVTLCYAPASQVGRGWRK 106

Query: 81  TEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYG 140
             DDL+KDK CQF+I ++PY+ +     K E+TV RD PTA+++VRAYA +++G  VAYG
Sbjct: 107 AHDDLSKDKACQFKIAQQPYDGAG----KFEYTVARDVPTASYYVRAYALDASGARVAYG 166

Query: 141 QTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKK 190
           +T         F V  ITG   S+++A+G  SAFSV +L  F +   ++  K
Sbjct: 167 ETAPSAS----FAVAGITGVTASIEVAAGVLSAFSVAALAVFLVLENKKKNK 210

[Close]

BLAST of Carg06757 vs. ExPASy TrEMBL
Match: A0A6J1GSA0 (High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111457061 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.7e-92
Identity = 175/176 (99.43%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 21  ITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 80
           +TASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED
Sbjct: 35  VTASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 94

Query: 81  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 140
           DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT
Sbjct: 95  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 154

Query: 141 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 197
           DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH
Sbjct: 155 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 210

[Close]

BLAST of Carg06757 vs. ExPASy TrEMBL
Match: A0A6J1K3M2 (High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111490359 PE=3 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 7.9e-90
Identity = 170/176 (96.59%), Postives = 174/176 (98.86%), Query Frame = 0


Query: 21  ITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 80
           +TASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED
Sbjct: 35  VTASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 94

Query: 81  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 140
           DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTV+RDTPTATFFVRAYAFNSAGDEVAYGQTT
Sbjct: 95  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVKRDTPTATFFVRAYAFNSAGDEVAYGQTT 154

Query: 141 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAAEGH 197
           DEKKGTNLFQVEAI+GRH SLDIAS CFSAFSVLSLTGFFIAGKR+AKKAAAAEGH
Sbjct: 155 DEKKGTNLFQVEAISGRHASLDIASACFSAFSVLSLTGFFIAGKRKAKKAAAAEGH 210

[Close]

BLAST of Carg06757 vs. ExPASy TrEMBL
Match: A0A6J1EDS1 (High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 PE=3 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 5.0e-76
Identity = 145/172 (84.30%), Postives = 155/172 (90.12%), Query Frame = 0


Query: 21  ITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 80
           +TASPK GQVLKAGVDKISV+WVLNG  K GSD +YK+V+ KLCYAPVSQVDRAWRKTED
Sbjct: 41  VTASPKPGQVLKAGVDKISVTWVLNGTVKSGSDSSYKTVEAKLCYAPVSQVDRAWRKTED 100

Query: 81  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 140
           DL+KDKTCQF IV KPYNP NKTVQK EWTV RD PT TFFVRAYAFNSAGDEV YGQTT
Sbjct: 101 DLSKDKTCQFLIVGKPYNPGNKTVQKFEWTVARDIPTGTFFVRAYAFNSAGDEVGYGQTT 160

Query: 141 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAA 193
           D KKGTNLFQ+EAITGRHISLDIAS CFSAFSVLSL+GFF+AGKR+AKKA A
Sbjct: 161 DNKKGTNLFQIEAITGRHISLDIASVCFSAFSVLSLSGFFLAGKRKAKKAEA 212

[Close]

BLAST of Carg06757 vs. ExPASy TrEMBL
Match: A0A5A7ULV7 (High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G004000 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.1e-74
Identity = 140/173 (80.92%), Postives = 152/173 (87.86%), Query Frame = 0


Query: 21  ITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 80
           +TASPK GQVLKAGVDKI V+WVLNG  K GSD +YK++K KLCYAPVSQVDRAWRKTED
Sbjct: 35  VTASPKPGQVLKAGVDKIRVTWVLNGTVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTED 94

Query: 81  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 140
           DL KDKTCQF IVEKPYNP+NKT Q  EWTVERD PT TFFVRAY FNSAG EVAYGQTT
Sbjct: 95  DLKKDKTCQFDIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTT 154

Query: 141 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAA 194
           D+KKGTNLFQ+E+ITGRH+SLDIAS CFSAFSV+SL GFF   KR+AKKAAAA
Sbjct: 155 DDKKGTNLFQIESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of Carg06757 vs. ExPASy TrEMBL
Match: A0A1S3AXC1 (High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.1e-74
Identity = 140/173 (80.92%), Postives = 152/173 (87.86%), Query Frame = 0


Query: 21  ITASPKSGQVLKAGVDKISVSWVLNGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTED 80
           +TASPK GQVLKAGVDKI V+WVLNG  K GSD +YK++K KLCYAPVSQVDRAWRKTED
Sbjct: 35  VTASPKPGQVLKAGVDKIRVTWVLNGTVKAGSDSSYKNIKAKLCYAPVSQVDRAWRKTED 94

Query: 81  DLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTT 140
           DL KDKTCQF IVEKPYNP+NKT Q  EWTVERD PT TFFVRAY FNSAG EVAYGQTT
Sbjct: 95  DLKKDKTCQFDIVEKPYNPANKTAQSFEWTVERDVPTGTFFVRAYVFNSAGQEVAYGQTT 154

Query: 141 DEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAKKAAAA 194
           D+KKGTNLFQ+E+ITGRH+SLDIAS CFSAFSV+SL GFF   KR+AKKAAAA
Sbjct: 155 DDKKGTNLFQIESITGRHVSLDIASVCFSAFSVVSLFGFFFIDKRKAKKAAAA 207

[Close]

BLAST of Carg06757 vs. TAIR 10
Match: AT5G50200.3 (nitrate transmembrane transporters )

HSP 1 Score: 186.0 bits (471), Expect = 2.8e-47
Identity = 95/163 (58.28%), Postives = 121/163 (74.23%), Query Frame = 0


Query: 28  GQVLKAGVDKISVSWVLNGAAKVGS--DLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKD 87
           G VL AG D ++++W L   + +GS  +  +K +KVKLCYAP SQVDR WRKT D+L KD
Sbjct: 49  GVVLDAGKDTLNITWTL---SSIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKD 108

Query: 88  KTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKG 147
           KTC  +I+ KPY   +KT+Q   WT+ERD PT T+FVRAYA ++ G EVAYGQ+TD+ K 
Sbjct: 109 KTCPHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKK 168

Query: 148 TNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 189
           TNLF V+AI+GRH SLDIAS CFS FSV++L  FF+  KR+AK
Sbjct: 169 TNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Carg06757 vs. TAIR 10
Match: AT5G50200.2 (nitrate transmembrane transporters )

HSP 1 Score: 186.0 bits (471), Expect = 2.8e-47
Identity = 95/163 (58.28%), Postives = 121/163 (74.23%), Query Frame = 0


Query: 28  GQVLKAGVDKISVSWVLNGAAKVGS--DLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKD 87
           G VL AG D ++++W L   + +GS  +  +K +KVKLCYAP SQVDR WRKT D+L KD
Sbjct: 49  GVVLDAGKDTLNITWTL---SSIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKD 108

Query: 88  KTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKG 147
           KTC  +I+ KPY   +KT+Q   WT+ERD PT T+FVRAYA ++ G EVAYGQ+TD+ K 
Sbjct: 109 KTCPHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKK 168

Query: 148 TNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 189
           TNLF V+AI+GRH SLDIAS CFS FSV++L  FF+  KR+AK
Sbjct: 169 TNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Carg06757 vs. TAIR 10
Match: AT5G50200.1 (nitrate transmembrane transporters )

HSP 1 Score: 186.0 bits (471), Expect = 2.8e-47
Identity = 95/163 (58.28%), Postives = 121/163 (74.23%), Query Frame = 0


Query: 28  GQVLKAGVDKISVSWVLNGAAKVGS--DLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKD 87
           G VL AG D ++++W L   + +GS  +  +K +KVKLCYAP SQVDR WRKT D+L KD
Sbjct: 49  GVVLDAGKDTLNITWTL---SSIGSKREAEFKIIKVKLCYAPPSQVDRPWRKTHDELFKD 108

Query: 88  KTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKG 147
           KTC  +I+ KPY   +KT+Q   WT+ERD PT T+FVRAYA ++ G EVAYGQ+TD+ K 
Sbjct: 109 KTCPHKIIAKPY---DKTLQSTTWTLERDIPTGTYFVRAYAVDAIGHEVAYGQSTDDAKK 168

Query: 148 TNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 189
           TNLF V+AI+GRH SLDIAS CFS FSV++L  FF+  KR+AK
Sbjct: 169 TNLFSVQAISGRHASLDIASICFSVFSVVALVVFFVNEKRKAK 205

[Close]

BLAST of Carg06757 vs. TAIR 10
Match: AT4G24730.4 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 181.4 bits (459), Expect = 6.9e-46
Identity = 96/179 (53.63%), Postives = 129/179 (72.07%), Query Frame = 0


Query: 12  TDLFSSLFCITASP-KSGQVLKAGVDKISVSWVL-NGAAKVGSDLAYKSVKVKLCYAPVS 71
           TDL +S+  +TA P K   VL+AG D ++++W L + +AKV +D A+K+++VKLCYAP+S
Sbjct: 52  TDLQNSIE-VTAKPVKDSGVLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPIS 111

Query: 72  QVDRAWRKTEDDLAKDKTCQFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNS 131
           QVDR WRKT++ L KD++C  +IV K Y   +KT Q  +WT+  D PT T+FVRAY  + 
Sbjct: 112 QVDRPWRKTDNKLFKDRSCPHEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRAYGIDG 171

Query: 132 AGDEVAYGQTTDEKKGTNLFQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 189
            G EVAYGQ+TDE + TNLF V AI+G H+ LDIAS  FS FSV+SL  FF+  KR+AK
Sbjct: 172 DGHEVAYGQSTDEGRTTNLFSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 226

[Close]

BLAST of Carg06757 vs. TAIR 10
Match: AT4G24730.1 (Calcineurin-like metallo-phosphoesterase superfamily protein )

HSP 1 Score: 176.0 bits (445), Expect = 2.9e-44
Identity = 88/160 (55.00%), Postives = 118/160 (73.75%), Query Frame = 0


Query: 30  VLKAGVDKISVSWVL-NGAAKVGSDLAYKSVKVKLCYAPVSQVDRAWRKTEDDLAKDKTC 89
           VL+AG D ++++W L + +AKV +D A+K+++VKLCYAP+SQVDR WRKT++ L KD++C
Sbjct: 282 VLEAGKDMVTITWKLKSSSAKVDTDTAFKTIQVKLCYAPISQVDRPWRKTDNKLFKDRSC 341

Query: 90  QFQIVEKPYNPSNKTVQKAEWTVERDTPTATFFVRAYAFNSAGDEVAYGQTTDEKKGTNL 149
             +IV K Y   +KT Q  +WT+  D PT T+FVRAY  +  G EVAYGQ+TDE + TNL
Sbjct: 342 PHEIVSKAY---DKTPQSLDWTIGLDIPTGTYFVRAYGIDGDGHEVAYGQSTDEGRTTNL 401

Query: 150 FQVEAITGRHISLDIASGCFSAFSVLSLTGFFIAGKRQAK 189
           F V AI+G H+ LDIAS  FS FSV+SL  FF+  KR+AK
Sbjct: 402 FSVHAISGHHVGLDIASTFFSVFSVVSLFVFFVMEKRKAK 438

The following BLAST results are available for this feature:

BLAST of Carg06757 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012276.1	1.5e-103	100.00	High-affinity nitrate transporter 3.1, partial [Cucurbita argyrosperma subsp. ar...	[more]
XP_022954957.1	3.5e-92	99.43	high-affinity nitrate transporter 3.1-like [Cucurbita moschata] >KAG6573089.1 Hi...	[more]
XP_023542774.1	1.8e-91	97.73	high-affinity nitrate transporter 3.1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022994709.1	1.6e-89	96.59	high-affinity nitrate transporter 3.1-like [Cucurbita maxima]	[more]
XP_022924070.1	1.0e-75	84.30	high-affinity nitrate transporter 3.1 [Cucurbita moschata]	[more]

BLAST of Carg06757 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGS5	4.0e-46	58.28	High-affinity nitrate transporter 3.1 OS=Arabidopsis thaliana OX=3702 GN=NRT3.1 ...	[more]
Q9SB67	9.8e-45	53.63	High-affinity nitrate transporter 3.2 OS=Arabidopsis thaliana OX=3702 GN=NRT3.2 ...	[more]
Q6ZI50	1.8e-38	45.74	High-affinity nitrate transporter-activating protein 2.1 OS=Oryza sativa subsp. ...	[more]
Q7XK12	5.2e-38	48.84	Probable high-affinity nitrate transporter-activating protein 2.2 OS=Oryza sativ...	[more]

BLAST of Carg06757 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GSA0	1.7e-92	99.43	High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111457061 ...	[more]
A0A6J1K3M2	7.9e-90	96.59	High-affinity nitrate transporter OS=Cucurbita maxima OX=3661 GN=LOC111490359 PE...	[more]
A0A6J1EDS1	5.0e-76	84.30	High-affinity nitrate transporter OS=Cucurbita moschata OX=3662 GN=LOC111431611 ...	[more]
A0A5A7ULV7	2.1e-74	80.92	High-affinity nitrate transporter OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3AXC1	2.1e-74	80.92	High-affinity nitrate transporter OS=Cucumis melo OX=3656 GN=LOC103483931 PE=3 S...	[more]

BLAST of Carg06757 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50200.3	2.8e-47	58.28	nitrate transmembrane transporters	[more]
AT5G50200.2	2.8e-47	58.28	nitrate transmembrane transporters	[more]
AT5G50200.1	2.8e-47	58.28	nitrate transmembrane transporters	[more]
AT4G24730.4	6.9e-46	53.63	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]
AT4G24730.1	2.9e-44	55.00	Calcineurin-like metallo-phosphoesterase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016605	High-affinity nitrate transporter	PIRSF	PIRSF012939	NAR2	coord: 6..191 e-value: 4.3E-78 score: 259.1
IPR016605	High-affinity nitrate transporter	PFAM	PF16974	NAR2	coord: 19..186 e-value: 4.1E-71 score: 238.0
IPR016605	High-affinity nitrate transporter	PANTHER	PTHR34806	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 10..190
None	No IPR available	PANTHER	PTHR34806:SF1	HIGH-AFFINITY NITRATE TRANSPORTER 3.2	coord: 10..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg06757-RA	Carg06757-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042128	nitrate assimilation
biological_process	GO:0015706	nitrate transport
biological_process	GO:0010167	response to nitrate
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0015112	nitrate transmembrane transporter activity