Carg05216 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg05216
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Lipase_3 domain-containing protein
Location	Carg_Chr07: 3568571 .. 3571167 (+)
RNA-Seq Expression	Carg05216
Synteny	Carg05216

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCCGTCGTGCTTATAACTGTAACAACGGCTTCTCCGACAGCTATATGCTTCTGAAGCCGGAAGAGGTGAAATTCTTGGACCTGTGGCGCCTCCTGTTTTCAAGTAACCTCAAGAAAAGGAGATTTTTAGATTCGAGTCACGCCAGAGAATACAATTTCTGGCATAGATTCTTCATCTTTCTCTCTATTGTCGTCCTCAAGCTCCTCGGATTTCTTGCCCACCCACTCGCCTTGTTCGGCTTCTACCTGGAGTCCTCCCTCAACGTTTTGTCAGCCAACCACGGCTTTAGAGGCATCCTCCTCAATATCTTGCGATGTAATTCCCCCCTCAGGTTTTGTTTTCCATCTCAAACAAATCCCTGTTTTCATACAAACCCAACTTTTTTTTTTCTTTGCTTTATGCAGTGAAGTTGAAAATACCGGACAGCTCATCAGCAGAGTACCTGTCGTTGATTGGTCATTTGGATAGCAGAGTAACGTTGGACCAAAGCATAAAGCCTGGCGATGTCAACTACTTTGGAGCTCTCTGCATGATGGCTTCTAAAGTCGTCTATGAGAATGAAGCACATGTTCTACAAACAGTCAACGATGTCTGGAAGGTGCACTCCATCACTTACCAATTATTTAAATTTTAGAATAAATTTGTAAGAGCTGGTTGAAACAATTAGATTACCAATCACAAAGTCTAAACTTTTGAAACAAATTTTTAGTCAAATGTTTTGCTCTCTAACTGATAATTTTTGTTGTTTAAATGTAGATGGAGTTCTTGGGATTTTTCAACTTTTGGAATAGTAAGAAGAGAATTACTCCTTTTCTGCTTTGCTTCTCAAATATATAATTGTTCTGTTTGTAGGCAACGGCAACTGACATATAATTGATAATGGTGTTCTTATTTGTATTTACTAGACTATCAAGAAAAATGCTCAACACAAGCATTCATGATGCGGGATAAAAGGGTTGATCACGACACCATAATTGTGTCTTTCAGAGGCACAGAGCCCTTCAATGCCGATGACTGGAGCTCCGACTTCGACATCTCTTGGTACGAGATCGAAGGCATAGGAAAAATCCATGGTGGATTCATGAAAGCACTTGGGTTGCAGAAGACCCATGGATGGCCCAAGGAAATAGACAGAGAAGATAAAAGAAGGCCTTTGGCTTACTACACCCTAAGAGAAAGGCTAAGAGAACTTCTCAAAGAAGATGAGAGAACAAGGTTTGTAGTGACAGGGCACAGCCTGGGCGGCGCTTTGGCCATTCTATTCCCTTCTATTTTGGCGTTCCATGAAGAGAAGTTGCTGCTGGAAAGATTAGAGGGAGTTTATACATTTGGACAACCTCGAGTTGGAGACAGCAAATTCGGAGAATTCATGGTGAAATCACTGTCGCAGTACAAGATTCGTTACTACAGATTCGTTTATGGTTTCGACATGGTCCCTCGCCTGCCCCTTGACAACAACACTTTGATGTACAAACACTTCGGTCCTTGCATTTATTTCAACTGGAACTATGTTGCGCAGGTAAATGGAGCTAACATTTTGGAATGAAACAAACCCTTTCTAACAACTAAAACAATATACTTTGTTTTTGCGCTTTCAGATTCTGGAAGAAGAGCCATTCAAGAACTATTTCTCAATCGTGGGAGCGGTACTGATGAGAATCCATGCATGTTTCGAGATAGGAAGAAGCTTCACAATTGGGTGGCGAAGGGGAAAGGAGTACGAGGAAAGAATGTGTTTTAGAATAGTGAGGTTGCTTGGTTTGTTATTGCCTGGAATTCCTGCTCATTGTCCTCAAGATTATGTTAATTCAACTCGCTTGGGTTCAACAGGTAGTCTTAACAGCGAGTATGATGTTAAAATTGAGTGAAGACTTTTCTTTTAAAAGAAAAAAAATTGCCATGTGATTTGAATGGCAGTTCAAAACATGGAGTACAATTTTAGCACCTGCCCCAAACCCAAAAGCCAGCTACAGTTTCTTATTTTACAACAATTGTACATTGCTTGCAACATCTAGGATGACACAGAACCAATCAAGCTACGCTGCTACTGTAAATTTCTAAGACGATAAAACTACCAGATGCTACCTTGAGAAATCAGGAAGCATGGCAGATGAACCAACGCTTGAGCGAACCAATCCACACCTGCTTGTGGAATGGGAGAAAGTTGTTAGTGTTGCAAACTCTCTGGTTCCGATCACCCCCTGACGAGTTCCTCATCTTGTGTGCTTTCATCTGTGCTTTCCTCAGCTTTTTCAATATCTTATCCATTGATGATGATCTTGCTCTTTCAAGTTTCATCTGAATCAACAAGGTTCATCATCAGCGAGCCACATAGCTACTTCTGGTTCTACTACATTCCCATTCTAATTTAACCAGGACCAACTTCTTATAATATTCTACGACTTCTTTATGTTTTCTCTTCAAGTTTTTTCCAATGAAACAGAGTGAAAATAGCAGATTTTGGGGCTAAAAGTGGCCCTACCTCAAGTTTGCGTATCGCAGCTTCAGCTTTTGCTTTCTGCAAATTCTCCCATGCTGTTATCTTGGCTTCTTCTCTCTGCAACCTAAACGTTACGTTTTGTATCATTGG

mRNA sequence

ATGGCTGCCCGTCGTGCTTATAACTGTAACAACGGCTTCTCCGACAGCTATATGCTTCTGAAGCCGGAAGAGGTGAAATTCTTGGACCTGTGGCGCCTCCTGTTTTCAAGTAACCTCAAGAAAAGGAGATTTTTAGATTCGAGTCACGCCAGAGAATACAATTTCTGGCATAGATTCTTCATCTTTCTCTCTATTGTCGTCCTCAAGCTCCTCGGATTTCTTGCCCACCCACTCGCCTTGTTCGGCTTCTACCTGGAGTCCTCCCTCAACGTTTTGTCAGCCAACCACGGCTTTAGAGGCATCCTCCTCAATATCTTGCGATTGAAGTTGAAAATACCGGACAGCTCATCAGCAGAGTACCTGTCGTTGATTGGTCATTTGGATAGCAGAGTAACGTTGGACCAAAGCATAAAGCCTGGCGATGTCAACTACTTTGGAGCTCTCTGCATGATGGCTTCTAAAGTCGTCTATGAGAATGAAGCACATGTTCTACAAACAGTCAACGATGTCTGGAAGATGGAGTTCTTGGGATTTTTCAACTTTTGGAATAACTATCAAGAAAAATGCTCAACACAAGCATTCATGATGCGGGATAAAAGGGTTGATCACGACACCATAATTGTGTCTTTCAGAGGCACAGAGCCCTTCAATGCCGATGACTGGAGCTCCGACTTCGACATCTCTTGGTACGAGATCGAAGGCATAGGAAAAATCCATGGTGGATTCATGAAAGCACTTGGGTTGCAGAAGACCCATGGATGGCCCAAGGAAATAGACAGAGAAGATAAAAGAAGGCCTTTGGCTTACTACACCCTAAGAGAAAGGCTAAGAGAACTTCTCAAAGAAGATGAGAGAACAAGGTTTGTAGTGACAGGGCACAGCCTGGGCGGCGCTTTGGCCATTCTATTCCCTTCTATTTTGGCGTTCCATGAAGAGAAGTTGCTGCTGGAAAGATTAGAGGGAGTTTATACATTTGGACAACCTCGAGTTGGAGACAGCAAATTCGGAGAATTCATGGTGAAATCACTGTCGCAGTACAAGATTCGTTACTACAGATTCGTTTATGGTTTCGACATGGTCCCTCGCCTGCCCCTTGACAACAACACTTTGATGTACAAACACTTCGGTCCTTGCATTTATTTCAACTGGAACTATGTTGCGCAGATTCTGGAAGAAGAGCCATTCAAGAACTATTTCTCAATCGTGGGAGCGGTACTGATGAGAATCCATGCATGTTTCGAGATAGGAAGAAGCTTCACAATTGGGTGGCGAAGGGGAAAGGAGTACGAGGAAAGAATGTGTTTTAGAATAGTGAGGTTGCTTGGTTTGTTATTGCCTGGAATTCCTGCTCATTGTCCTCAAGATTATGTTAATTCAACTCGCTTGGGTTCAACAGGTAGTCTTAACAGCGAGTATGATGTTAAAATTGAGTGAAGACTTTTCTTTTAAAAGAAAAAAAATTGCCATGTGATTTGAATGGCAGTTCAAAACATGGAGTACAATTTTAGCACCTGCCCCAAACCCAAAAGCCAGCTACAGTTTCTTATTTTACAACAATTGTACATTGCTTGCAACATCTAGGATGACACAGAACCAATCAAGCTACGCTGCTACTGTAAATTTCTAAGACGATAAAACTACCAGATGCTACCTTGAGAAATCAGGAAGCATGGCAGATGAACCAACGCTTGAGCGAACCAATCCACACCTGCTTGTGGAATGGGAGAAAGTTGTTAGTGTTGCAAACTCTCTGGTTCCGATCACCCCCTGACGAGTTCCTCATCTTGTGTGCTTTCATCTGTGCTTTCCTCAGCTTTTTCAATATCTTATCCATTGATGATGATCTTGCTCTTTCAAGTTTCATCTGAATCAACAAGGTTCATCATCAGCGAGCCACATAGCTACTTCTGGTTCTACTACATTCCCATTCTAATTTAACCAGGACCAACTTCTTATAATATTCTACGACTTCTTTATGTTTTCTCTTCAAGTTTTTTCCAATGAAACAGAGTGAAAATAGCAGATTTTGGGGCTAAAAGTGGCCCTACCTCAAGTTTGCGTATCGCAGCTTCAGCTTTTGCTTTCTGCAAATTCTCCCATGCTGTTATCTTGGCTTCTTCTCTCTGCAACCTAAACGTTACGTTTTGTATCATTGG

Coding sequence (CDS)

ATGGCTGCCCGTCGTGCTTATAACTGTAACAACGGCTTCTCCGACAGCTATATGCTTCTGAAGCCGGAAGAGGTGAAATTCTTGGACCTGTGGCGCCTCCTGTTTTCAAGTAACCTCAAGAAAAGGAGATTTTTAGATTCGAGTCACGCCAGAGAATACAATTTCTGGCATAGATTCTTCATCTTTCTCTCTATTGTCGTCCTCAAGCTCCTCGGATTTCTTGCCCACCCACTCGCCTTGTTCGGCTTCTACCTGGAGTCCTCCCTCAACGTTTTGTCAGCCAACCACGGCTTTAGAGGCATCCTCCTCAATATCTTGCGATTGAAGTTGAAAATACCGGACAGCTCATCAGCAGAGTACCTGTCGTTGATTGGTCATTTGGATAGCAGAGTAACGTTGGACCAAAGCATAAAGCCTGGCGATGTCAACTACTTTGGAGCTCTCTGCATGATGGCTTCTAAAGTCGTCTATGAGAATGAAGCACATGTTCTACAAACAGTCAACGATGTCTGGAAGATGGAGTTCTTGGGATTTTTCAACTTTTGGAATAACTATCAAGAAAAATGCTCAACACAAGCATTCATGATGCGGGATAAAAGGGTTGATCACGACACCATAATTGTGTCTTTCAGAGGCACAGAGCCCTTCAATGCCGATGACTGGAGCTCCGACTTCGACATCTCTTGGTACGAGATCGAAGGCATAGGAAAAATCCATGGTGGATTCATGAAAGCACTTGGGTTGCAGAAGACCCATGGATGGCCCAAGGAAATAGACAGAGAAGATAAAAGAAGGCCTTTGGCTTACTACACCCTAAGAGAAAGGCTAAGAGAACTTCTCAAAGAAGATGAGAGAACAAGGTTTGTAGTGACAGGGCACAGCCTGGGCGGCGCTTTGGCCATTCTATTCCCTTCTATTTTGGCGTTCCATGAAGAGAAGTTGCTGCTGGAAAGATTAGAGGGAGTTTATACATTTGGACAACCTCGAGTTGGAGACAGCAAATTCGGAGAATTCATGGTGAAATCACTGTCGCAGTACAAGATTCGTTACTACAGATTCGTTTATGGTTTCGACATGGTCCCTCGCCTGCCCCTTGACAACAACACTTTGATGTACAAACACTTCGGTCCTTGCATTTATTTCAACTGGAACTATGTTGCGCAGATTCTGGAAGAAGAGCCATTCAAGAACTATTTCTCAATCGTGGGAGCGGTACTGATGAGAATCCATGCATGTTTCGAGATAGGAAGAAGCTTCACAATTGGGTGGCGAAGGGGAAAGGAGTACGAGGAAAGAATGTGTTTTAGAATAGTGAGGTTGCTTGGTTTGTTATTGCCTGGAATTCCTGCTCATTGTCCTCAAGATTATGTTAATTCAACTCGCTTGGGTTCAACAGGTAGTCTTAACAGCGAGTATGATGTTAAAATTGAGTGA

Protein sequence

MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFNFWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE

Homology

[Close]

BLAST of Carg05216 vs. NCBI nr
Match: KAG6595033.1 (Lipase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027056.1 Lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 987.3 bits (2551), Expect = 4.8e-284
Identity = 477/477 (100.00%), Postives = 477/477 (100.00%), Query Frame = 0


Query: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 478
           IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 477

[Close]

BLAST of Carg05216 vs. NCBI nr
Match: XP_023518931.1 (uncharacterized protein LOC111782304 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 981.5 bits (2536), Expect = 2.6e-282
Identity = 475/477 (99.58%), Postives = 476/477 (99.79%), Query Frame = 0


Query: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSS AREYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSRAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSIVVLKLLGFLA+PLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSIVVLKLLGFLANPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 478
           IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 477

[Close]

BLAST of Carg05216 vs. NCBI nr
Match: XP_022962880.1 (uncharacterized protein LOC111463247 [Cucurbita moschata])

HSP 1 Score: 980.7 bits (2534), Expect = 4.5e-282
Identity = 474/477 (99.37%), Postives = 475/477 (99.58%), Query Frame = 0


Query: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNCNNGFSDSYMLLKPEE KFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSI+VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFL FFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 478
           IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 477

[Close]

BLAST of Carg05216 vs. NCBI nr
Match: XP_023003657.1 (uncharacterized protein LOC111497186 [Cucurbita maxima])

HSP 1 Score: 974.9 bits (2519), Expect = 2.4e-280
Identity = 471/477 (98.74%), Postives = 474/477 (99.37%), Query Frame = 0


Query: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNC+NGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF
Sbjct: 1   MAARRAYNCSNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGW KEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWTKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFP+ILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPTILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 478
           IGWRRGKEYEERMCFRIVRL GLLLPGIPAHCPQDYVN+TRLGST SLNSEYDVKIE
Sbjct: 421 IGWRRGKEYEERMCFRIVRLFGLLLPGIPAHCPQDYVNATRLGSTVSLNSEYDVKIE 477

[Close]

BLAST of Carg05216 vs. NCBI nr
Match: XP_008440283.1 (PREDICTED: uncharacterized protein LOC107990286 [Cucumis melo])

HSP 1 Score: 853.2 bits (2203), Expect = 1.1e-243
Identity = 410/473 (86.68%), Postives = 435/473 (91.97%), Query Frame = 0


Query: 7   YNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSIV 66
           YNCN GFS SYMLL PEEVKFLDL RLLFSSNLKKRRF+DSSHARE+NFWHRFFIFLSIV
Sbjct: 17  YNCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIV 76

Query: 67  VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGH 126
           VLKLL F   PLAL GF+LESSLN LS N GF GILLN  RL+LKIPDSSSAEYLS+IGH
Sbjct: 77  VLKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGH 136

Query: 127 LDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFNFWNNYQ 186
           LDSRVTLD SIKPGDVNYFGALCMMASK+ YENEAHV Q V DVWKMEFLGFFNFWN+YQ
Sbjct: 137 LDSRVTLDSSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQ 196

Query: 187 EKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 246
           EKCSTQAFMMRDK+VDHDTI+VSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL
Sbjct: 197 EKCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 256

Query: 247 GLQKTHGWPKEIDRE--DKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFP 306
           GLQK  GWPKEI+R+  D+RRPLAYYTLRE+L+EL+KE+ERTRFVVTGHSLGGALAILFP
Sbjct: 257 GLQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFP 316

Query: 307 SILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLP 366
           SIL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL+QYKIRYYRFVYGFDMVPRLP
Sbjct: 317 SILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLP 376

Query: 367 LDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWR 426
           LD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTI WR
Sbjct: 377 LDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWR 436

Query: 427 RGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 478
           RGKEYEE++  RIVRL GLLLPGIPAHCPQDYVNSTRLGST    SEYDVK++
Sbjct: 437 RGKEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGSTSHFLSEYDVKVQ 489

[Close]

BLAST of Carg05216 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 374.8 bits (961), Expect = 1.5e-102
Identity = 208/482 (43.15%), Postives = 279/482 (57.88%), Query Frame = 0


Query: 57  HRFFIFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSS 116
           HR+ IF+SI+V KL+     P+   G+ +E  LN+ S N  F G+L NIL  K+ +P   
Sbjct: 85  HRWVIFVSIIVRKLIAIFGKPMEWTGYLVEFFLNLFSLNGNFLGLLYNILHGKVVMPHRG 144

Query: 117 SAEYLSLIGHLDSRVTLDQS----IKPGDVNYFGA----------LCMMASKVVYENEAH 176
           S  ++S IGHLD R+ L +S     + G+ +++            LCMMASK+ YENE  
Sbjct: 145 SETFISAIGHLDGRINLYKSETLTKEIGEPDFWQKIGIGHRDLMDLCMMASKLAYENEKV 204

Query: 177 VLQTVNDVWKMEFLGFFNFWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWS 236
           V   VN  WKM F+ F+N WN+++++ STQ F++ DK  D + I+VSFRGTEPF+ADDW 
Sbjct: 205 VRNVVNLHWKMHFVDFYNCWNDFEKEMSTQVFLLCDKPKDANLILVSFRGTEPFDADDWI 264

Query: 237 SDFDISWYEIEGIGKIHGGFMKALG-----------------------LQKTHGW--PKE 296
           +DFD SWYEI  +GK+H GF++ALG                       L+  H    P E
Sbjct: 265 TDFDYSWYEIPKLGKVHMGFLEALGLGNRTNASTFHEQLFVNNLKFANLENVHATIPPSE 324

Query: 297 IDR-----------------EDKRRP---------------LAYYTLRERLRELLKEDER 356
             +                  D  RP                AYY +R +L+ LLKE + 
Sbjct: 325 SSKSSTSFSDSDAHTGSDLSSDSERPTDTRKKKFRLEIPERTAYYVVRSKLKRLLKEHKN 384

Query: 357 TRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQ 416
            +FVVTGHSLGGALAILFP++L  HEE  ++ERL G+YT+GQPRVG+ + G FM   L  
Sbjct: 385 AKFVVTGHSLGGALAILFPAVLVLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLEH 444

Query: 417 YKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAV 468
              +Y+R VY  D+VPRLP DN T ++KHFG C Y+N  Y+ Q + EEP  NYF +   V
Sbjct: 445 PVPKYFRVVYCNDLVPRLPYDNKTFLFKHFGICQYYNSLYIEQNINEEPNPNYFGMRFLV 504

[Close]

BLAST of Carg05216 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.8e-100
Identity = 201/502 (40.04%), Postives = 297/502 (59.16%), Query Frame = 0


Query: 10  NNGFSDSYMLLKPEEVKFLDLWRL-LFSSNLKKRRFLDSSHAREYNFW--------HRFF 69
           N G   +Y++++P    ++DL+R  +      K +FL+    RE++          HR+ 
Sbjct: 12  NPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWV 71

Query: 70  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 129
           I +SI+V K++  L  P+   GF ++  LN+ SAN GF G+LL +++ K+ IP+  S  +
Sbjct: 72  IVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVTF 131

Query: 130 LSLIGHLDSRVTLDQ------------SIKPGDVNY-FGA-----LCMMASKVVYENEAH 189
           +S IG LD R++L +            S+  G V    G+     LC+MASK+ YEN   
Sbjct: 132 VSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKV 191

Query: 190 VLQTVNDVWKMEFLGFFNFWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWS 249
           V   V+  WKM  + F + WN+YQ++ STQ F+  DK+ D + I++SFRGTEPF+ADDW 
Sbjct: 192 VENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWG 251

Query: 250 SDFDISWYEIEGIGKIHGGFMKALGLQKTHGW---------PKEIDREDKRRPL------ 309
           +DFD SWYE+  +GK+H GF++A+GL                   + E+ ++ L      
Sbjct: 252 TDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVER 311

Query: 310 -AYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGVYTFG 369
            AYY +R  L+ LL E E  RFVVTGHSLGGALAILFP++L  +EE  +++RL GVYTFG
Sbjct: 312 SAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFG 371

Query: 370 QPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGPCIYFNWNYV 429
           QPR+G+ + G FM   L+Q   RY+R VY  D+VPRLP D+ T +YKHFG C++++  Y 
Sbjct: 372 QPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYN 431

Query: 430 AQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKEYEERMCFRIVRLLGLLLP 469
               E+EP  N + +   +L  + A +E+ R  T+G+  G +Y+E     + RL+GL++P
Sbjct: 432 ETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIP 491

[Close]

BLAST of Carg05216 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 3.6e-85
Identity = 191/506 (37.75%), Postives = 275/506 (54.35%), Query Frame = 0


Query: 14  SDSYMLLKPEEVKFLDLWRLLFSSNL-KKRRFLDSSHAR------EYNFWHRFFIFLSIV 73
           S S++++ P+E  FLDL++ +  S+L    +F  +S  R       Y+ W      +S+ 
Sbjct: 9   STSHLIVSPDEGTFLDLFKHIVLSDLGSGAKFFRASDQRVPATAAYYSRWP-----VSVF 68

Query: 74  VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGH 133
           + K+L     P A+ G   +  LN    NHGF GIL NI  ++LKIP    A+++S IG+
Sbjct: 69  ICKILQLFQMPAAMLGHLTDFLLNFYYQNHGFLGILRNIFLIRLKIPKRGEADFISTIGY 128

Query: 134 LDSRVTL------------------DQSIKPG-DVNYFGA--------LCMMASKVVYEN 193
           LDSR+ L                  + S+K G +    GA        LC+MASK+ YEN
Sbjct: 129 LDSRMDLHGTPMVSHQADEVISNADNPSLKEGHNSKIKGALGNRSLMDLCIMASKLAYEN 188

Query: 194 EAHVLQTVNDVWKMEFLGFFNFWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNAD 253
              V + V + WKM F+  +   N +Q+  +T AF+  DK  D + I++SFRGT PF+  
Sbjct: 189 TKVVERVVAEHWKMHFVADYGGMNYFQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIP 248

Query: 254 DWSSDFDISWYEIEGIGKIHGGFMKALGL--------------QKTHGWPKEIDREDKRR 313
           +W +DFD S   +   G +H GF++A+GL               K+ G   E+ +E +  
Sbjct: 249 NWCTDFDFSLVGLGDAGSVHVGFLEAMGLGHRNSISSFETSINTKSPGSITELRKESEMA 308

Query: 314 P--------LAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLE 373
           P          Y+     L+ LLK+ +  +FVVTGHSLGGALAILF  IL   +E  +L+
Sbjct: 309 PDHLVWAYDGVYFLAASTLKGLLKDHKNAKFVVTGHSLGGALAILFTCILEIQQETEVLD 368

Query: 374 RLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGP 433
           RL  VYTFGQPR+G+   G FM   L+  + RY+R VY  DMVPR+P D+    ++HFG 
Sbjct: 369 RLLNVYTFGQPRIGNYNLGYFMQNRLNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGT 428

Query: 434 CIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKEYEERMCFRI 464
           CIY++  +     +EEP +N F I  A+   I A +E+ RSF +    G EY+E    R+
Sbjct: 429 CIYYDSRFFGYFTKEEPSRNPFGIENAISAHITAWWELWRSFILNHVYGAEYKETWESRM 488

[Close]

BLAST of Carg05216 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 9.4e-09
Identity = 53/175 (30.29%), Postives = 79/175 (45.14%), Query Frame = 0


Query: 206 IIVSFRGTEPFNADDWSSDFDISWYEIEGI---GKIHGGFMKALGLQKTHGWPKEIDRED 265
           I++SFRG+   + ++W  + +    EI  I    + H GF        T  W    D   
Sbjct: 98  IVLSFRGSR--SIENWIGNLNFDLKEINDICSGCRGHDGF--------TSSWRSVAD--- 157

Query: 266 KRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGV 325
                   TLR+++ + ++E    R V TGHSLGGALA +  + L  +   +       V
Sbjct: 158 --------TLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYDI------DV 217

Query: 326 YTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGP 378
           +++G PRVG+  F EF+     Q     YR  +  D+VPRLP       Y H  P
Sbjct: 218 FSYGAPRVGNRAFAEFLT---VQTGGTLYRITHTNDIVPRLP--PREFGYSHSSP 240

[Close]

BLAST of Carg05216 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 62.8 bits (151), Expect = 1.2e-08
Identity = 54/163 (33.13%), Postives = 75/163 (46.01%), Query Frame = 0


Query: 205 TIIVSFRGTEPFNADDWSSDF---DISWYEIEGIGKIHGGFMKALGLQKTHGWPKEIDRE 264
           TI + FRG+      +W +D     +S+  + G  K+H GF+ + G         E+  E
Sbjct: 168 TIYIVFRGSSSIR--NWIADLTFVPVSYPPVSGT-KVHKGFLDSYG---------EVQNE 227

Query: 265 DKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEG 324
                     L   + +  K+    +  VTGHSLGGA A+L    L   EE L    L  
Sbjct: 228 ----------LVATVLDQFKQYPSYKVAVTGHSLGGATALLCALDLYQREEGLSSSNL-F 287

Query: 325 VYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLP 365
           +YT GQPRVGD  F  ++V +     I Y R V   D+VP LP
Sbjct: 288 LYTQGQPRVGDPAFANYVVST----GIPYRRTVNERDIVPHLP 303

[Close]

BLAST of Carg05216 vs. ExPASy TrEMBL
Match: A0A6J1HGB4 (uncharacterized protein LOC111463247 OS=Cucurbita moschata OX=3662 GN=LOC111463247 PE=4 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 2.2e-282
Identity = 474/477 (99.37%), Postives = 475/477 (99.58%), Query Frame = 0


Query: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNCNNGFSDSYMLLKPEE KFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF
Sbjct: 1   MAARRAYNCNNGFSDSYMLLKPEEEKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSI+VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSILVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFL FFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLEFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 478
           IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE
Sbjct: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 477

[Close]

BLAST of Carg05216 vs. ExPASy TrEMBL
Match: A0A6J1KN75 (uncharacterized protein LOC111497186 OS=Cucurbita maxima OX=3661 GN=LOC111497186 PE=4 SV=1)

HSP 1 Score: 974.9 bits (2519), Expect = 1.2e-280
Identity = 471/477 (98.74%), Postives = 474/477 (99.37%), Query Frame = 0


Query: 1   MAARRAYNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60
           MAARRAYNC+NGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF
Sbjct: 1   MAARRAYNCSNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFF 60

Query: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120
           IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY
Sbjct: 61  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 120

Query: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180
           LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN
Sbjct: 121 LSLIGHLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFN 180

Query: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240
           FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG
Sbjct: 181 FWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHG 240

Query: 241 GFMKALGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300
           GFMKALGLQKTHGW KEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA
Sbjct: 241 GFMKALGLQKTHGWTKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALA 300

Query: 301 ILFPSILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360
           ILFP+ILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV
Sbjct: 301 ILFPTILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMV 360

Query: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420
           PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT
Sbjct: 361 PRLPLDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFT 420

Query: 421 IGWRRGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 478
           IGWRRGKEYEERMCFRIVRL GLLLPGIPAHCPQDYVN+TRLGST SLNSEYDVKIE
Sbjct: 421 IGWRRGKEYEERMCFRIVRLFGLLLPGIPAHCPQDYVNATRLGSTVSLNSEYDVKIE 477

[Close]

BLAST of Carg05216 vs. ExPASy TrEMBL
Match: A0A1S3B1H5 (uncharacterized protein LOC107990286 OS=Cucumis melo OX=3656 GN=LOC107990286 PE=4 SV=1)

HSP 1 Score: 853.2 bits (2203), Expect = 5.2e-244
Identity = 410/473 (86.68%), Postives = 435/473 (91.97%), Query Frame = 0


Query: 7   YNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSIV 66
           YNCN GFS SYMLL PEEVKFLDL RLLFSSNLKKRRF+DSSHARE+NFWHRFFIFLSIV
Sbjct: 17  YNCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIV 76

Query: 67  VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGH 126
           VLKLL F   PLAL GF+LESSLN LS N GF GILLN  RL+LKIPDSSSAEYLS+IGH
Sbjct: 77  VLKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGH 136

Query: 127 LDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFNFWNNYQ 186
           LDSRVTLD SIKPGDVNYFGALCMMASK+ YENEAHV Q V DVWKMEFLGFFNFWN+YQ
Sbjct: 137 LDSRVTLDSSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQ 196

Query: 187 EKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 246
           EKCSTQAFMMRDK+VDHDTI+VSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL
Sbjct: 197 EKCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 256

Query: 247 GLQKTHGWPKEIDRE--DKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFP 306
           GLQK  GWPKEI+R+  D+RRPLAYYTLRE+L+EL+KE+ERTRFVVTGHSLGGALAILFP
Sbjct: 257 GLQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFP 316

Query: 307 SILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLP 366
           SIL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL+QYKIRYYRFVYGFDMVPRLP
Sbjct: 317 SILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLP 376

Query: 367 LDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWR 426
           LD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTI WR
Sbjct: 377 LDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWR 436

Query: 427 RGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 478
           RGKEYEE++  RIVRL GLLLPGIPAHCPQDYVNSTRLGST    SEYDVK++
Sbjct: 437 RGKEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGSTSHFLSEYDVKVQ 489

[Close]

BLAST of Carg05216 vs. ExPASy TrEMBL
Match: A0A0A0KFS7 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490940 PE=4 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 2.7e-240
Identity = 402/472 (85.17%), Postives = 436/472 (92.37%), Query Frame = 0


Query: 7   YNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSIV 66
           YNCN GFS+SYMLL PEEVKFLDL+RLLFSSNLKKRRF+DSSHARE+NFWHRFFIFLSIV
Sbjct: 16  YNCNGGFSNSYMLLNPEEVKFLDLFRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIV 75

Query: 67  VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGH 126
           VLKLL F   PLAL GF+LESSLN LS N GF GILLN  RL+LKIP++SSAEYLS+IGH
Sbjct: 76  VLKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPNTSSAEYLSMIGH 135

Query: 127 LDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFNFWNNYQ 186
           LDSRVTLD+SIKPGDVNYFGALCMMASK+ YENEAHV Q V DVWKMEFLGFFNFWN+YQ
Sbjct: 136 LDSRVTLDRSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQ 195

Query: 187 EKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 246
           EKCSTQAFMMRD++ +HDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL
Sbjct: 196 EKCSTQAFMMRDRKDNHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 255

Query: 247 GLQKTHGWPKEIDRE-DKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPS 306
           GLQK  GWPKE++R+  +R PLAYYTLRE+L+EL+KE+ERTRFVVTGHSLGGALAILFPS
Sbjct: 256 GLQKCVGWPKEMERKGHERHPLAYYTLREKLKELMKENERTRFVVTGHSLGGALAILFPS 315

Query: 307 ILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPL 366
           IL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL++YKIRYYRFVYGFDMVPRLPL
Sbjct: 316 ILVFHEEKLLLERLEGVYTFGQPRVGDRTFGEFMVKSLAEYKIRYYRFVYGFDMVPRLPL 375

Query: 367 DNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRR 426
           D+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTI WRR
Sbjct: 376 DDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWRR 435

Query: 427 GKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGSTGSLNSEYDVKIE 478
           GKEYEE++  RIVRL GLLLPGIPAHCPQDYVNSTRLGST   ++EYDVK+E
Sbjct: 436 GKEYEEKVFLRIVRLFGLLLPGIPAHCPQDYVNSTRLGSTTPFHTEYDVKVE 487

[Close]

BLAST of Carg05216 vs. ExPASy TrEMBL
Match: A0A5D3CNF4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G004760 PE=4 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 3.9e-239
Identity = 403/461 (87.42%), Postives = 427/461 (92.62%), Query Frame = 0


Query: 7   YNCNNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSIV 66
           YNCN GFS SYMLL PEEVKFLDL RLLFSSNLKKRRF+DSSHARE+NFWHRFFIFLSIV
Sbjct: 14  YNCNGGFSSSYMLLNPEEVKFLDLLRLLFSSNLKKRRFVDSSHAREHNFWHRFFIFLSIV 73

Query: 67  VLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGH 126
           VLKLL F   PLAL GF+LESSLN LS N GF GILLN  RL+LKIPDSSSAEYLS+IGH
Sbjct: 74  VLKLLRFFDKPLALLGFFLESSLNFLSLNGGFSGILLNFFRLRLKIPDSSSAEYLSMIGH 133

Query: 127 LDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFNFWNNYQ 186
           LDSRVTLD+SIKPGDVNYFGALCMMASK+ YENEAHV Q V DVWKMEFLGFFNFWN+YQ
Sbjct: 134 LDSRVTLDRSIKPGDVNYFGALCMMASKLSYENEAHVAQIVKDVWKMEFLGFFNFWNDYQ 193

Query: 187 EKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 246
           EKCSTQAFMMRDK+VDHDTI+VSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL
Sbjct: 194 EKCSTQAFMMRDKKVDHDTIVVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKAL 253

Query: 247 GLQKTHGWPKEIDRE--DKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFP 306
           GLQK  GWPKEI+R+  D+RRPLAYYTLRE+L+EL+KE+ERTRFVVTGHSLGGALAILFP
Sbjct: 254 GLQKCIGWPKEIERQGHDERRPLAYYTLREKLKELVKENERTRFVVTGHSLGGALAILFP 313

Query: 307 SILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLP 366
           SIL FHEEKLLLERLEGVYTFGQPRVGD  FGEFMVKSL+QYKIRYYRFVYGFDMVPRLP
Sbjct: 314 SILVFHEEKLLLERLEGVYTFGQPRVGDRAFGEFMVKSLAQYKIRYYRFVYGFDMVPRLP 373

Query: 367 LDNNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWR 426
           LD+  LM+KHFGPCIYF+WNYVAQILEEEPFKNYFSIVGA+LMRIHAC EIGRSFTI WR
Sbjct: 374 LDDKALMFKHFGPCIYFDWNYVAQILEEEPFKNYFSIVGAILMRIHACLEIGRSFTISWR 433

Query: 427 RGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGST 466
           RGKEYEE++  RIVRL GLLLPGI AHCPQDYVNSTRLGST
Sbjct: 434 RGKEYEEKVFLRIVRLFGLLLPGILAHCPQDYVNSTRLGST 474

[Close]

BLAST of Carg05216 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 468.8 bits (1205), Expect = 5.3e-132
Identity = 232/454 (51.10%), Postives = 318/454 (70.04%), Query Frame = 0


Query: 17  YMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYNFWHRFFIFLSIVVLKLLGFLAH 76
           Y++L+PEE++  +L RLLFS +++K R +DSS   E++F HR+ IF+S+V+LKLL F + 
Sbjct: 13  YLILRPEELRPWELVRLLFSGDIEKPRSVDSSETEEHSFRHRWLIFVSLVLLKLLRFFSK 72

Query: 77  PLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGHLDSRVTLDQS 136
            LAL G  LE SLN LS N+ F G+    LR ++ +P  +S  Y S IGHLD+RV+LD +
Sbjct: 73  LLALVGSALEFSLNFLS-NNSFSGL---FLRGEVVMPQRTSENYQSFIGHLDTRVSLDMT 132

Query: 137 IKPGD-VNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFNFWNNYQEKCSTQAFM 196
           +   D   Y+ AL +MASK+ YEN A +   V + W M++LG  ++WN YQEK +TQAF+
Sbjct: 133 LNREDGEKYYAALSIMASKIAYENAARIKHVVENHWNMKYLGLVDYWNEYQEKETTQAFI 192

Query: 197 MRDKRV------DHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALGLQ 256
           M              T++V+FRGTE FN++DW SDFDI+W+E+  IG IHGGFMKALGLQ
Sbjct: 193 MSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELPNIGNIHGGFMKALGLQ 252

Query: 257 KTHGWPKE-IDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILA 316
               WPKE +   D++ PLAYY++R+ L+ L+ +++ T+FV+TGHSLGGALAILF ++L 
Sbjct: 253 NNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGHSLGGALAILFTAVLV 312

Query: 317 FHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNN 376
            H E  LLER++GVYT+GQPRVGDSKFGEFM K L +Y I+YYRFVY  D+VPRLP D+ 
Sbjct: 313 IHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRFVYNNDIVPRLPYDDK 372

Query: 377 TLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKE 436
            LM+KHFG CIY++ NY A+++ E+  +N+F + G + M   A  E  RSFTI   +G E
Sbjct: 373 DLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAILEFIRSFTIVAEKGSE 432

Query: 437 YEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRL 463
           Y E    +  R LG+++PG+  H PQDYVN+TRL
Sbjct: 433 YSEGWLLKGGRALGIIVPGVSNHTPQDYVNATRL 462

[Close]

BLAST of Carg05216 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 442.2 bits (1136), Expect = 5.3e-124
Identity = 219/455 (48.13%), Postives = 312/455 (68.57%), Query Frame = 0


Query: 16  SYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSSHAREYN----FWHRFFIFLSIVVLKLL 75
           +Y +L P E    DL  LLFSS+L  R+F+ SS  R  +    F  R+ IF+SIV+ KL+
Sbjct: 6   NYFVLDPREATVSDLIHLLFSSDLGDRKFIHSSEERIEDDLSRFRRRWIIFVSIVIQKLI 65

Query: 76  GFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGHLDSRV 135
                PL   GF L   LN+LS+N GF  IL N+ +  +  P+ +SA + SL G+LD RV
Sbjct: 66  ILFKKPLYFVGFSLACWLNLLSSNGGFLMILPNLFKGNIIWPEKASATFASLNGNLDRRV 125

Query: 136 TLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFNFWNNYQEKCST 195
            L+  ++ G   Y   L +MASK+ YEN   V   +++ WKM+ LGF++ WN YQ++ ST
Sbjct: 126 ELNPKVERGSKRYKAMLSIMASKLSYENINFVSSVLHNHWKMDLLGFYSCWNGYQKQRST 185

Query: 196 QAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALGLQKT 255
           +  +++D   D + IIVSFRGT+PF+ADDW +D D+SWYE++ +GKIHGGFMKALGLQK 
Sbjct: 186 EVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMKALGLQK- 245

Query: 256 HGWPKEIDREDKRRP---LAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILA 315
            GWPKE++ ++ +      AYYT+R  L+E+L ++  ++F++TGHSLGGALAILF ++L 
Sbjct: 246 EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAILFTAVLV 305

Query: 316 FHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNN 375
            H+E+ +LERLEGVYTFGQPRVGD +FG FM  SL ++ ++Y R+VY  DMVPRLP D+ 
Sbjct: 306 MHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVPRLPFDDK 365

Query: 376 TLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKE 435
           TLM+KHFG C+Y++  Y  ++ EEEP KNYF++V  +   ++A +E+ RSF + + +G E
Sbjct: 366 TLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVMPYWKGGE 425

Query: 436 YEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLG 464
           + E    R  R++ LL+PG+PAH P +Y+N T LG
Sbjct: 426 FREGWFLRCFRVVALLIPGLPAHFPNEYINVTLLG 459

[Close]

BLAST of Carg05216 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 421.4 bits (1082), Expect = 9.7e-118
Identity = 217/458 (47.38%), Postives = 308/458 (67.25%), Query Frame = 0


Query: 16  SYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSS----HAREYNFWHRFFIFLSIVVLKLL 75
           +Y +L P E    DL RLLF S+L+ R+F+D+S      R   F  R+ IF+SIVV KLL
Sbjct: 6   NYFVLDPREATVTDLMRLLFYSDLENRKFVDTSVENLENRLCEFRGRWIIFVSIVVQKLL 65

Query: 76  GFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIGHLDSRV 135
             L  PL+  GF L   LN+ S+N GF  I LN+++ +   P+ +SA + S+ G+LD +V
Sbjct: 66  IILRKPLSFLGFALGFWLNLPSSNGGFFKIFLNLVKGRFIWPEKTSATFASINGNLDQKV 125

Query: 136 T--LDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFNFWNNYQEKC 195
              L +SIK GD  Y   L +MASK+ YENE  +   + D W+M+ LGF++  N++ +  
Sbjct: 126 ELGLGRSIKIGDERYKPLLSIMASKLAYENEHFIRSVLQDHWQMDLLGFYSCPNDFDQTR 185

Query: 196 STQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKALGLQ 255
           ST+  ++RD + + + I+VSFRGT+PFNADDW +D D+SW+ +  +GKIHGGFMKALGL 
Sbjct: 186 STEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKALGLP 245

Query: 256 KTHGWPKEID---REDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSI 315
           K  GW +EI+    ++K   LAYYT+  +L+E+ +++  ++F+++GHSLGGALAILF ++
Sbjct: 246 K-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILFTAV 305

Query: 316 LAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLD 375
           L  H+EK +LERLEGVYTFGQPRVGD  FG +M   L ++ ++Y R+VY  DMVPRLP D
Sbjct: 306 LIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRLPFD 365

Query: 376 NNTLMYKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRG 435
           + TLM+KHFG C+Y +  Y  ++ EEEP KNYF+I   +   I+A +E+ RSF I   +G
Sbjct: 366 DKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISCWQG 425

Query: 436 KEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLGS 465
           +EY E       RL+ LL+PG+PAH P +YVN   LG+
Sbjct: 426 REYREGWLLTCFRLVALLIPGLPAHFPNEYVNVALLGN 462

[Close]

BLAST of Carg05216 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 414.5 bits (1064), Expect = 1.2e-115
Identity = 213/459 (46.41%), Postives = 294/459 (64.05%), Query Frame = 0


Query: 10  NNGFSDSYMLLKPEEVKFLDLWRLLFSSNLKKRRFLDSS----HAREYNFWHRFFIFLSI 69
           N  F +SY L+ P +  FLDL  LLFSSNL   RF+DS          +F  R+ + L+I
Sbjct: 5   NMKFCNSYFLVDPTKASFLDLLLLLFSSNLTSARFIDSPPDTLKGFRRSFASRWILALAI 64

Query: 70  VVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEYLSLIG 129
            + K+L  L+ P A  G  L   LN+L+AN GF  ++LN++  KL  PD SSA Y S IG
Sbjct: 65  FLQKVLMLLSKPFAFIGQKLTYWLNLLTANGGFFNLILNLMSGKLVKPDKSSATYTSFIG 124

Query: 130 HLDSRVTLDQSIKPGDVNYFGALCMMASKVVYENEAHVLQTVNDVWKMEFLGFFNFWNNY 189
             D R+ LD+ I  G + Y   L +MASK+ YE++ ++   V + WKM+ +G ++F+N +
Sbjct: 125 CSDRRIELDEKINVGSIEYKSMLSIMASKISYESKPYITSVVKNTWKMDLVGNYDFYNAF 184

Query: 190 QEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWSSDFDISWYEIEGIGKIHGGFMKA 249
           QE   TQAF+ +    + D I+VSFRGTEPF A DW +D D+SWYE++ +GK+H GF +A
Sbjct: 185 QESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSRA 244

Query: 250 LGLQKTHGWPKEIDREDKRRPLAYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPS 309
           LGLQK  GWPKE          AYYT+R+ LR+ L  ++  ++++TGHSLGGALA LFP+
Sbjct: 245 LGLQK-DGWPKE--NISLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALFPA 304

Query: 310 ILAFHEEKLLLERLEGVYTFGQPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPL 369
           ILA H E  LL++LEG+YTFGQPRVGD  FGEFM   + ++ I Y RFVY  D+VPR+P 
Sbjct: 305 ILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRVPF 364

Query: 370 DNNTLM-YKHFGPCIYFNWNYVAQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWR 429
           D+  L  YKH+GPC  FN  Y  ++ E+ P  NYF+++  +   +   +E  RSF + + 
Sbjct: 365 DDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQFW 424

Query: 430 RGKEYEERMCFRIVRLLGLLLPGIPAHCPQDYVNSTRLG 464
           +G EY+E    R VR++G++ PG   H P DYVNSTRLG
Sbjct: 425 KGDEYKENWLMRFVRVVGIVFPGGSNHFPFDYVNSTRLG 460

[Close]

BLAST of Carg05216 vs. TAIR 10
Match: AT3G14360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-101
Identity = 201/502 (40.04%), Postives = 297/502 (59.16%), Query Frame = 0


Query: 10  NNGFSDSYMLLKPEEVKFLDLWRL-LFSSNLKKRRFLDSSHAREYNFW--------HRFF 69
           N G   +Y++++P    ++DL+R  +      K +FL+    RE++          HR+ 
Sbjct: 12  NPGQVSNYLIVRPHRGGYIDLFRYGVRDDQTSKAKFLEMPDNREWSTITIDEEAEDHRWV 71

Query: 70  IFLSIVVLKLLGFLAHPLALFGFYLESSLNVLSANHGFRGILLNILRLKLKIPDSSSAEY 129
           I +SI+V K++  L  P+   GF ++  LN+ SAN GF G+LL +++ K+ IP+  S  +
Sbjct: 72  IVVSILVRKIIRLLRTPMEFTGFVVDFFLNLFSANGGFFGLLLRLIQAKVVIPERGSVTF 131

Query: 130 LSLIGHLDSRVTLDQ------------SIKPGDVNY-FGA-----LCMMASKVVYENEAH 189
           +S IG LD R++L +            S+  G V    G+     LC+MASK+ YEN   
Sbjct: 132 VSTIGQLDGRISLYKEWNFVEHLEGIDSVDSGRVKIELGSRGLMDLCVMASKLAYENAKV 191

Query: 190 VLQTVNDVWKMEFLGFFNFWNNYQEKCSTQAFMMRDKRVDHDTIIVSFRGTEPFNADDWS 249
           V   V+  WKM  + F + WN+YQ++ STQ F+  DK+ D + I++SFRGTEPF+ADDW 
Sbjct: 192 VENVVDLHWKMNLVEFLDCWNDYQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWG 251

Query: 250 SDFDISWYEIEGIGKIHGGFMKALGLQKTHGW---------PKEIDREDKRRPL------ 309
           +DFD SWYE+  +GK+H GF++A+GL                   + E+ ++ L      
Sbjct: 252 TDFDYSWYEVPNVGKLHMGFLEAMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVER 311

Query: 310 -AYYTLRERLRELLKEDERTRFVVTGHSLGGALAILFPSILAFHEEKLLLERLEGVYTFG 369
            AYY +R  L+ LL E E  RFVVTGHSLGGALAILFP++L  +EE  +++RL GVYTFG
Sbjct: 312 SAYYAVRVILKRLLSEHENARFVVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFG 371

Query: 370 QPRVGDSKFGEFMVKSLSQYKIRYYRFVYGFDMVPRLPLDNNTLMYKHFGPCIYFNWNYV 429
           QPR+G+ + G FM   L+Q   RY+R VY  D+VPRLP D+ T +YKHFG C++++  Y 
Sbjct: 372 QPRIGNREVGLFMKAQLNQPVDRYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYN 431

Query: 430 AQILEEEPFKNYFSIVGAVLMRIHACFEIGRSFTIGWRRGKEYEERMCFRIVRLLGLLLP 469
               E+EP  N + +   +L  + A +E+ R  T+G+  G +Y+E     + RL+GL++P
Sbjct: 432 ETKAEDEPDPNPYGLRYKILGHVIAVWELVRGLTMGYTHGPDYKEGWFRILFRLMGLVIP 491

The following BLAST results are available for this feature:

BLAST of Carg05216 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6595033.1	4.8e-284	100.00	Lipase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027056.1 Lipase, pa...	[more]
XP_023518931.1	2.6e-282	99.58	uncharacterized protein LOC111782304 [Cucurbita pepo subsp. pepo]	[more]
XP_022962880.1	4.5e-282	99.37	uncharacterized protein LOC111463247 [Cucurbita moschata]	[more]
XP_023003657.1	2.4e-280	98.74	uncharacterized protein LOC111497186 [Cucurbita maxima]	[more]
XP_008440283.1	1.1e-243	86.68	PREDICTED: uncharacterized protein LOC107990286 [Cucumis melo]	[more]

BLAST of Carg05216 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1S3ZP85	1.5e-102	43.15	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
F4JFU8	1.8e-100	40.04	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	3.6e-85	37.75	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]
O59952	9.4e-09	30.29	Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1	[more]
P19515	1.2e-08	33.13	Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2	[more]

BLAST of Carg05216 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HGB4	2.2e-282	99.37	uncharacterized protein LOC111463247 OS=Cucurbita moschata OX=3662 GN=LOC1114632...	[more]
A0A6J1KN75	1.2e-280	98.74	uncharacterized protein LOC111497186 OS=Cucurbita maxima OX=3661 GN=LOC111497186...	[more]
A0A1S3B1H5	5.2e-244	86.68	uncharacterized protein LOC107990286 OS=Cucumis melo OX=3656 GN=LOC107990286 PE=...	[more]
A0A0A0KFS7	2.7e-240	85.17	Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G490940 PE...	[more]
A0A5D3CNF4	3.9e-239	87.42	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of Carg05216 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67050.1	5.3e-132	51.10	alpha/beta-Hydrolases superfamily protein	[more]
AT5G42930.1	5.3e-124	48.13	alpha/beta-Hydrolases superfamily protein	[more]
AT1G56630.1	9.7e-118	47.38	alpha/beta-Hydrolases superfamily protein	[more]
AT1G45201.1	1.2e-115	46.41	triacylglycerol lipase-like 1	[more]
AT3G14360.1	1.3e-101	40.04	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 131..405 e-value: 1.0E-47 score: 165.2
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 148..405
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 207..367 e-value: 4.5E-38 score: 130.3
None	No IPR available	PANTHER	PTHR46086:SF17	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 9..466
None	No IPR available	CDD	cd00519	Lipase_3	coord: 148..382 e-value: 7.83979E-49 score: 165.342
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 9..466

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg05216-RA	Carg05216-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004806	triglyceride lipase activity