Carg05106 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg05106
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionDNA-directed RNA polymerases II and IV subunit 5A-like
LocationCarg_Chr08: 1628658 .. 1632998 (-)
RNA-Seq ExpressionCarg05106
SyntenyCarg05106
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCTCCATCTCTCTCCGTCTTCATCGTTCCACAAAACCACCGCCGCCGCCGCCGCCCAACCACGAAATCTCTGTCTCTCCCTTTCTCTATCTTGTAGCCGTAACTCTGTTGAGTAAGGTTTGTTTTTGTCTGAGAATTTGTGATCTGAGTCGGGTTGCTGAGTGTAGTTCTTTTTCTAGTTTTTGATGCTGTTGTATGTTGATTTATAAATTTACCTCGTTTGCGAAGATTCTTGCAGGTTCCGCTCTTGATATTACTCTGGTTCTCGTAATCTGTTCTTCACTCTTTTGATTACTGAATTTTTCCTTTTTGCTTTTGGTTTGGCTTGATAGTGTTGGTTCCTATAATGTTTGTAAATATACTTTAGGGTTGGTATAATCTCTAGAGATGGTTTGAGTTGAAAGATTGCACTTGTTTTTCCTATGCCCTTTTGCTGCCTCCTGTTGGCCTAGTCTCTTTAAATATTTTACCTTCAGCTGAGTGTTTCCCAAAGGTTATTCAGAGGCTTATTATTAACTCATTTGGACTTTTCTGTGGTAATTCTGTGGTGGCTCGACTTTTCTGGGTAGTGATTCTACTCTTGATGTTTTTTGGAAACAAATTGGATTGTTTTAGGCTACTTGGTGTTGTTGCCCTCTTCTGGTAACTATGGTTTATCACAAATGTTTGCTAATTGGGAATCCTTTTTGTATATCTTCCTGTTAGTAATGAGATATTTTGTTTCCTATTCGAAGAAGGGGGGAAAAGGTAACTAATTTTAGGGAGAATGGGTTCAAGCCATAGTGGTCACCAATTTAAGATTTAATATCCTACTAGTTTCTTTGGCAACGTAGTGTAATAGGATCAAACGGTTGTTCTATAAGATATTTTGAGTTTACAAGGTAAATCAATGTGGTCGACTAAGGATATTAAGTCTTTTGGATGCAAAATCATCATGGTGAACTTGTTGTCTTGTAGGGTTGGTTGAGGTGTACTGTAGTGGATTCAAACATCCATAGTTATTTAGAATAAAAAGAAGAAAAATGTTTATATATACCGCATGTGTGAGATCCCATATCGATTAGGGAGGAGAACAAAACATTCTTTGTAAGGGTGTGGAAACCTAGTAGACGCCTTTTAAAAACCTTGAGGGAAAGCCCAAAGAGGACAATATCTACTAGCGGTGGGCTTGGACGATTACAAATGGTATCAGAACTAGATACCAGGCGATGTGCCAGTGAGGACGCTGGTCCCCGAAGGGAGGTGGATTGGGAGGTCCCACATCGGTTGGAGAAGAGAACGAGTGCCAGTGAGAACGCTGAGCCCTGAATGGGGGTGGATTGTGTGATCCCACATCGGTTGGGGAGGAAAACGAAACATTCTTTATAAGGGTGTGGAAACCTTTACCTAGTAGACGCGTTTTAAAAACCTTGAGGGAAGCCCGAAAGGGGAAGCTCAAACAGGACAATATCTGCTAGCGGTGGGCTTGGGCTTGGGCTTGGGCTATTACAACATTTTTGAAAATTTTTGTGGCTTGTTTGTGCTTCTCCAGCATGATTTAATCTTTTTCTTTAAACTGTTGTCAATATTCATAGTGTGTTCTATTTGTCATCTTGTTCTCTTTGACTTAGGGATTAAGGGCGCCAGCAAGCTCTAACCAAATTTGCTTTCTTGTTCTCGCATTGTCGGTGTCTCCAACTTGTTTTGAATTTTCTGAAAATTGTTGTTCTATTATATCCATAACGTGTTCTATATGCTGTGTTTTGCATTCGTTTTGAAATTTCCAATATGTAACATAATTAATCAGATTGATGTTTCTACTACTGAATTTTACCTCATTTAGAATCTGCATTATCTATTTCTTGTGTACTTATTTGGAGCTTAGGTATTGAGTGTTGAATTGTTTGACAGTGGTTTAGCTACAGACAAGATGGTGCTAGCAGAGGAGGAAATCACCAGGCTTTTCAGAGTTCGAAAAACTGTGTTACAAATGCTTAAAGACCGAGGCTACTTCGTTGGAGACTTTGAGGTTGACATGTCCAGAGAGCAATTTAAGAATAAATATGGTGAAAGCATGAAACGAGAAGATATCGTGATCAACAAAGCGAAGAGAAATGATAGCTCTGATCAGGTATTGTCTCAGACAAGGGGCTGCAAGTTTCTCGTTAATTCTTCTATTTGTTTCCTTCCTCTTATAGTGACTCTTTTCTGTATAGATATACATTTTCTTTCCAGAAGAACCGAAGGTTGGCGTCAAGACAATGAAAACGTACACCAACCGCATGAAATCGGAGAATGTTTTTAGAGCAATATTGGTCGTTCAACAGAATTTGACCCCTTTCGCTCGCACCTGTATAAGTGAAATATCTACCAAATTCCACTTGGAAGTTTTCCAGGTGAAAACATTTCTTTAACATATTTTATGGAGTGTTTTTAGTTTTTTTATTTTTTTTATTTTTTAAGAATCGAGTCCTTTTGAGAAACATGAAAAAATACAAAGACACGAAAACAAAGTCCAAACAAAAGGAGTCCAAAACAAGTTACTTGTGAGATCCTACATCGGTTGGAGAGGGGAACGAAGCATTTCTTTCAAGGATGTGGAGACTCTCCCTAGCAGACACATTTTAAAACCCTTGAGGGGAAGCCCGAGAGGGAAAGACCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGGCTAGTACAACTCCAATCTAAAAGAACAAGGTCAAGATCATAAGTACAAAATGGCTTAGTCACTTGGCCCCAAAGGGAGGCATTAAACGTAGCAACCTCGCACACTTCCTCACCCGATCTCTTGGCCTCTATAAAAGTTCCATTGTACCTTTCCGACCGAACACTTAACAAACAGCAAAGAAGTTAGCCCACATGACTTTGCCTCTTTCCCTAAAAAGGGAAAAGCTCCTCATCCAACGATGAACGATCATCCCTACCTCGAGCTCCAGAATCCCAAAAAGAGGATTTTAACCAATATCCCAATGAAAGTAAGCAAACTGACAATCCCACAACAAATGATCAAGGTGCTCAAACGAAAAGGAGGAAGAATGCCAAGCCACCGGTCGGAGAAGTAAATTAGACGGGTCATTTACCAATAATTTGAGGAGAACCTACTCTGCAAACTAAAAATTATGTTATGCTCTCTTCCTTTATTCCTTGTTTTGCAATATGAGAATGAAAAATATGGGGTTTCTACATTCATGCTCCCACTGTCTTAGTTCACCTTTAGATATAACTTTTTTAACCCTTTCTTCATTTGTTGATGCAGGAAGCAGAATTACTAGTTAATATCAAAGATCACGTTCTTGTTCCTGAGCACCAGTTACTTTCCAACGAAGAGAAAAAGACACTACTGGAGCGATATACCGTGAAAGAAACACAGGTTAGCATTAATTCTTCAGAAAATGTTACGTTGTTAGAATGCATCTCAAATATTGATATTGAAATTTGATATGCTCATACGTTCCTGATACTGCTTTGGACTTCTACAACGTTATCTCAACTATTGATTAACTACTCGGTTTTCTTTGTATACTATGTTCAAATTTATCTGTCATGTCTCAAGCCCACCGCTAGTAGATATTGTCTTCTTTGGGCTTATCCTTTCGGGCTTCCCCTCAAGGTTTTTAAAACGTCTGCTAGGGAGAGGTTTCCACACCCTTGTAAAGAATGGTTCATTCTCCTCCCCAATCGACGTGGGATCTCACATTATCATTCCTATTTAGTGTAATTTTTTCTGAGTTTTGGATTTCATTAGATACATAGCATGCCGAGTCCTGCAAACTATGCGCTTATTTATTGATTTTGCCCAATAACCGAAGTATTCTGGTCTTACTCTGTGCATTTTGTTCATGTCGATTCCTCCTAAAAGGCAAGATTTTAAGTTATTAAGCTTTCTCAGCTCTAAAGTTGGAACCTAAAACCTCGTAGAGTATCAACGAACATGCTAAGCTCGTAGCATGGAGACATCCCTTGGGGGTATTAACACATTTTTGTTACAAAATGAATGAGAATCTTATATGGTGTTGCATATTTTGTGTTAGCTACCGCGCATACAGGTGACCGACCCAGTTGCGAGATACTACGGGCTAAAGCGTGGACAAGTTGTGAAGATCATCAGACCAAGTGAGACTGCAGGAAGATATGTAACTTACAGATATGTTGTCTGAGTATAATTATTATTTTGCAACCGAAGATGTAACATATAAATGCACATTGAACTCATCTTTTCAAGTCTTCATCACTTTGGGAACTTCTGTATTTGGAATTTGAATAGGAATATTTATTTATTTTTATTAAAGGGTACTTTTGTAAATTGCCCCTATGGAATGGATTTTATTAAAGGTTA

mRNA sequence

CCTCCATCTCTCTCCGTCTTCATCGTTCCACAAAACCACCGCCGCCGCCGCCGCCCAACCACGAAATCTCTGTCTCTCCCTTTCTCTATCTTGTAGCCGTAACTCTGTTGAGTAAGTGGTTTAGCTACAGACAAGATGGTGCTAGCAGAGGAGGAAATCACCAGGCTTTTCAGAGTTCGAAAAACTGTGTTACAAATGCTTAAAGACCGAGGCTACTTCGTTGGAGACTTTGAGGTTGACATGTCCAGAGAGCAATTTAAGAATAAATATGGTGAAAGCATGAAACGAGAAGATATCGTGATCAACAAAGCGAAGAGAAATGATAGCTCTGATCAGGAAGCAGAATTACTAGTTAATATCAAAGATCACGTTCTTGTTCCTGAGCACCAGTTACTTTCCAACGAAGAGAAAAAGACACTACTGGAGCGATATACCGTGAAAGAAACACAGCTACCGCGCATACAGGTGACCGACCCAGTTGCGAGATACTACGGGCTAAAGCGTGGACAAGTTGTGAAGATCATCAGACCAAGTGAGACTGCAGGAAGATATGTAACTTACAGATATGTTGTCTGAGTATAATTATTATTTTGCAACCGAAGATGTAACATATAAATGCACATTGAACTCATCTTTTCAAGTCTTCATCACTTTGGGAACTTCTGTATTTGGAATTTGAATAGGAATATTTATTTATTTTTATTAAAGGGTACTTTTGTAAATTGCCCCTATGGAATGGATTTTATTAAAGGTTA

Coding sequence (CDS)

ATGGTGCTAGCAGAGGAGGAAATCACCAGGCTTTTCAGAGTTCGAAAAACTGTGTTACAAATGCTTAAAGACCGAGGCTACTTCGTTGGAGACTTTGAGGTTGACATGTCCAGAGAGCAATTTAAGAATAAATATGGTGAAAGCATGAAACGAGAAGATATCGTGATCAACAAAGCGAAGAGAAATGATAGCTCTGATCAGGAAGCAGAATTACTAGTTAATATCAAAGATCACGTTCTTGTTCCTGAGCACCAGTTACTTTCCAACGAAGAGAAAAAGACACTACTGGAGCGATATACCGTGAAAGAAACACAGCTACCGCGCATACAGGTGACCGACCCAGTTGCGAGATACTACGGGCTAAAGCGTGGACAAGTTGTGAAGATCATCAGACCAAGTGAGACTGCAGGAAGATATGTAACTTACAGATATGTTGTCTGA

Protein sequence

MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKRNDSSDQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRGQVVKIIRPSETAGRYVTYRYVV
Homology
BLAST of Carg05106 vs. NCBI nr
Match: KAG7025474.1 (DNA-directed RNA polymerases II and IV subunit 5A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 279.6 bits (714), Expect = 1.5e-71
Identity = 146/146 (100.00%), Postives = 146/146 (100.00%), Query Frame = 0

Query: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60
           MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNDSSDQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 120
           RNDSSDQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG
Sbjct: 61  RNDSSDQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 120

Query: 121 LKRGQVVKIIRPSETAGRYVTYRYVV 147
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 121 LKRGQVVKIIRPSETAGRYVTYRYVV 146

BLAST of Carg05106 vs. NCBI nr
Match: XP_022959553.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959554.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959555.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_023004795.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004796.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004797.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023513561.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo] >XP_023513563.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 252.3 bits (643), Expect = 2.6e-63
Identity = 146/206 (70.87%), Postives = 146/206 (70.87%), Query Frame = 0

Query: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60
           MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNDSSD------------------------------------------------------ 120
           RNDSSD                                                      
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 ------QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 147
                 QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

BLAST of Carg05106 vs. NCBI nr
Match: ESR64457.1 (hypothetical protein CICLE_v10009504mg [Citrus clementina])

HSP 1 Score: 248.4 bits (633), Expect = 3.7e-62
Identity = 124/146 (84.93%), Postives = 141/146 (96.58%), Query Frame = 0

Query: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60
           M L++EEI RLFR+R+TV+QML+DRGYFVGDFE++MS+EQF  K+GE+MKRED+VINKA 
Sbjct: 1   MTLSDEEIKRLFRIRRTVMQMLRDRGYFVGDFEINMSKEQFIAKFGENMKREDLVINKAL 60

Query: 61  RNDSSDQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 120
           RNDSSDQEAELLVNIK+HVLVPEHQ+L+NEEKKTLL+RYTVKETQLPRIQVTDP+ARYYG
Sbjct: 61  RNDSSDQEAELLVNIKEHVLVPEHQVLTNEEKKTLLKRYTVKETQLPRIQVTDPIARYYG 120

Query: 121 LKRGQVVKIIRPSETAGRYVTYRYVV 147
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 121 LKRGQVVKIIRPSETAGRYVTYRYVV 146

BLAST of Carg05106 vs. NCBI nr
Match: KAF3965687.1 (hypothetical protein CMV_010147 [Castanea mollissima])

HSP 1 Score: 247.7 bits (631), Expect = 6.3e-62
Identity = 123/146 (84.25%), Postives = 138/146 (94.52%), Query Frame = 0

Query: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60
           MV++E+E  RLFR+RKTV+QMLKDRGY V DFEVDM+++QF+ KYGESMKRED+VINK K
Sbjct: 1   MVVSEDETNRLFRIRKTVMQMLKDRGYLVADFEVDMTKDQFRRKYGESMKREDLVINKTK 60

Query: 61  RNDSSDQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 120
           R DSSDQEAEL+VNIK+HVLVPEHQ+L+ EEKKTLLERYTVKETQLPRIQVTDP+ARYYG
Sbjct: 61  RTDSSDQEAELMVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYYG 120

Query: 121 LKRGQVVKIIRPSETAGRYVTYRYVV 147
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 121 LKRGQVVKIIRPSETAGRYVTYRYVV 146

BLAST of Carg05106 vs. NCBI nr
Match: XP_022991826.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023547519.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 246.9 bits (629), Expect = 1.1e-61
Identity = 140/206 (67.96%), Postives = 146/206 (70.87%), Query Frame = 0

Query: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60
           MVL+EEEITRLFR+RKTVLQMLKDRGYFVGDFE+DMSREQFK+KYGESMKREDIVINKAK
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDIVINKAK 60

Query: 61  RNDSSD------------------------------------------------------ 120
           RNDSSD                                                      
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 ------QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 147
                 QEAELLVN+KDHVLVPEHQLL+NEEKKTLLERYTVKETQLPRIQVTDPVARYYG
Sbjct: 121 FHLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

BLAST of Carg05106 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 2.0e-50
Identity = 111/205 (54.15%), Postives = 135/205 (65.85%), Query Frame = 0

Query: 2   VLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKR 61
           +L EEE+ RL+R++KT++QML+DRGYF+ D E+ M+++QF  K+G++MKRED+V  KAKR
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 62  NDSSD------------------------------------------------------- 121
           ND+SD                                                       
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120

Query: 122 -----QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 147
                QEAE+LVNIK+HVLVPEHQ+L+ EEKKTLLERYTVKETQLPRIQVTDP+ARY+GL
Sbjct: 121 HLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGL 180

BLAST of Carg05106 vs. ExPASy Swiss-Prot
Match: Q9N5K2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis elegans OX=6239 GN=rpb-5 PE=3 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.0e-30
Identity = 88/207 (42.51%), Postives = 108/207 (52.17%), Query Frame = 0

Query: 5   EEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGE-----SMKREDIVINKA 64
           E E  RL+R+RKTVLQM+ DRGY V   E+D   E FK +YG+        R D+ I  A
Sbjct: 5   ELETYRLWRIRKTVLQMVHDRGYLVAQDELDQPLETFKVQYGDRPSEKKPARSDLTILVA 64

Query: 65  KRNDSSDQ---------------------------------------------------- 124
             +D +DQ                                                    
Sbjct: 65  HNDDPADQMFVFFPEDAKIGIKTIKAICQQMQEQNISRAIIVVQTGMTPSAKQSIGDMAP 124

Query: 125 --------EAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYY 147
                   EAEL+VNI +H LVPEH +++ EEK  LL RY +K++QLPRIQ  DPVARY+
Sbjct: 125 KYMLEHFLEAELMVNITEHELVPEHVVMTAEEKAELLARYKLKDSQLPRIQQCDPVARYF 184

BLAST of Carg05106 vs. ExPASy Swiss-Prot
Match: A8XGH1 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis briggsae OX=6238 GN=rpb-5 PE=3 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 2.3e-30
Identity = 87/207 (42.03%), Postives = 107/207 (51.69%), Query Frame = 0

Query: 5   EEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGE-----SMKREDIVINKA 64
           E E  RL+R+RKTVLQM+ DRGY V   E+D   E FK  +G+        R D+ I  A
Sbjct: 5   ELETYRLWRIRKTVLQMVHDRGYLVAQEELDQPLETFKETFGDRPSEKKPARSDLTILVA 64

Query: 65  KRNDSSDQ---------------------------------------------------- 124
             +D +DQ                                                    
Sbjct: 65  HNDDPADQMFVFFPEDTKIGIKTIKAICQQMQEQNISRAIIVVQTGMTPSAKQSIGDMAP 124

Query: 125 --------EAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYY 147
                   EAEL+VNI +H LVPEH +++ EEK  LL RY +K++QLPRIQ  DPVARY+
Sbjct: 125 KYMLEHFLEAELMVNITEHELVPEHVVMTAEEKAELLARYKLKDSQLPRIQQCDPVARYF 184

BLAST of Carg05106 vs. ExPASy Swiss-Prot
Match: Q80UW8 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10090 GN=Polr2e PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 5.6e-29
Identity = 79/206 (38.35%), Postives = 108/206 (52.43%), Query Frame = 0

Query: 5   EEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGES-----MKREDIVINKA 64
           EEE  RL+++RKT++Q+  DRGY V   E+D + E+FK ++G+       +R D+ +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  KRNDSSDQ---------------------------------------------------- 124
             +D +DQ                                                    
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 --------EAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYY 146
                   + ELL+NI +H LVPEH +++ EE   LL RY ++E+QLPRIQ  DPVARY+
Sbjct: 124 KYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARYF 183

BLAST of Carg05106 vs. ExPASy Swiss-Prot
Match: B0BNE2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus OX=10116 GN=Polr2e PE=2 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 5.6e-29
Identity = 79/206 (38.35%), Postives = 108/206 (52.43%), Query Frame = 0

Query: 5   EEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGES-----MKREDIVINKA 64
           EEE  RL+++RKT++Q+  DRGY V   E+D + E+FK ++G+       +R D+ +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  KRNDSSDQ---------------------------------------------------- 124
             +D +DQ                                                    
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 --------EAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYY 146
                   + ELL+NI +H LVPEH +++ EE   LL RY ++E+QLPRIQ  DPVARY+
Sbjct: 124 KYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQLPRIQAGDPVARYF 183

BLAST of Carg05106 vs. ExPASy TrEMBL
Match: A0A6J1KT45 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111497988 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.2e-63
Identity = 146/206 (70.87%), Postives = 146/206 (70.87%), Query Frame = 0

Query: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60
           MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNDSSD------------------------------------------------------ 120
           RNDSSD                                                      
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 ------QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 147
                 QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

BLAST of Carg05106 vs. ExPASy TrEMBL
Match: A0A6J1H699 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=3662 GN=LOC111460591 PE=3 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.2e-63
Identity = 146/206 (70.87%), Postives = 146/206 (70.87%), Query Frame = 0

Query: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60
           MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNDSSD------------------------------------------------------ 120
           RNDSSD                                                      
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 ------QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 147
                 QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

BLAST of Carg05106 vs. ExPASy TrEMBL
Match: V4UEF9 (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10009504mg PE=3 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.8e-62
Identity = 124/146 (84.93%), Postives = 141/146 (96.58%), Query Frame = 0

Query: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60
           M L++EEI RLFR+R+TV+QML+DRGYFVGDFE++MS+EQF  K+GE+MKRED+VINKA 
Sbjct: 1   MTLSDEEIKRLFRIRRTVMQMLRDRGYFVGDFEINMSKEQFIAKFGENMKREDLVINKAL 60

Query: 61  RNDSSDQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 120
           RNDSSDQEAELLVNIK+HVLVPEHQ+L+NEEKKTLL+RYTVKETQLPRIQVTDP+ARYYG
Sbjct: 61  RNDSSDQEAELLVNIKEHVLVPEHQVLTNEEKKTLLKRYTVKETQLPRIQVTDPIARYYG 120

Query: 121 LKRGQVVKIIRPSETAGRYVTYRYVV 147
           LKRGQVVKIIRPSETAGRYVTYRYVV
Sbjct: 121 LKRGQVVKIIRPSETAGRYVTYRYVV 146

BLAST of Carg05106 vs. ExPASy TrEMBL
Match: A0A6J1JRV4 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111488357 PE=3 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 5.2e-62
Identity = 140/206 (67.96%), Postives = 146/206 (70.87%), Query Frame = 0

Query: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60
           MVL+EEEITRLFR+RKTVLQMLKDRGYFVGDFE+DMSREQFK+KYGESMKREDIVINKAK
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDIVINKAK 60

Query: 61  RNDSSD------------------------------------------------------ 120
           RNDSSD                                                      
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 ------QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 147
                 QEAELLVN+KDHVLVPEHQLL+NEEKKTLLERYTVKETQLPRIQVTDPVARYYG
Sbjct: 121 FHLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

BLAST of Carg05106 vs. ExPASy TrEMBL
Match: A0A6J1GQK9 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=3662 GN=LOC111456196 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 6.8e-62
Identity = 139/206 (67.48%), Postives = 146/206 (70.87%), Query Frame = 0

Query: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60
           MVL+EEEITRLFR+RKTVLQMLKDRGYFVGDFE+DMSREQFK+KYGESMKRED+VINKAK
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDVVINKAK 60

Query: 61  RNDSSD------------------------------------------------------ 120
           RNDSSD                                                      
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 ------QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 147
                 QEAELLVN+KDHVLVPEHQLL+NEEKKTLLERYTVKETQLPRIQVTDPVARYYG
Sbjct: 121 FHLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 180

BLAST of Carg05106 vs. TAIR 10
Match: AT3G22320.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 199.9 bits (507), Expect = 1.4e-51
Identity = 111/205 (54.15%), Postives = 135/205 (65.85%), Query Frame = 0

Query: 2   VLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKR 61
           +L EEE+ RL+R++KT++QML+DRGYF+ D E+ M+++QF  K+G++MKRED+V  KAKR
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 62  NDSSD------------------------------------------------------- 121
           ND+SD                                                       
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120

Query: 122 -----QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGL 147
                QEAE+LVNIK+HVLVPEHQ+L+ EEKKTLLERYTVKETQLPRIQVTDP+ARY+GL
Sbjct: 121 HLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQLPRIQVTDPIARYFGL 180

BLAST of Carg05106 vs. TAIR 10
Match: AT5G57980.1 (RNA polymerase II fifth largest subunit, C )

HSP 1 Score: 111.3 bits (277), Expect = 6.6e-25
Identity = 72/206 (34.95%), Postives = 108/206 (52.43%), Query Frame = 0

Query: 5   EEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESM---KREDIVINKAKR 64
           ++EITR+F+VR+TVLQML+DRGY + + ++++ RE+F  ++ ++M    +E + ++  K 
Sbjct: 5   DDEITRIFKVRRTVLQMLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVSANKG 64

Query: 65  NDSSD------------------------------------------------------- 124
            + +D                                                       
Sbjct: 65  PNPADKIYVFYPEGPKVGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSELNKM 124

Query: 125 ------QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYG 147
                 +EAEL+ NI +H LV ++ +L ++ KK LL  YTV++TQLPRI VTDP+ARYYG
Sbjct: 125 LTIEVFEEAELVTNITEHKLVNKYYVLDDQAKKKLLNTYTVQDTQLPRILVTDPLARYYG 184

BLAST of Carg05106 vs. TAIR 10
Match: AT2G41340.1 (RNA polymerase II fifth largest subunit, D )

HSP 1 Score: 92.0 bits (227), Expect = 4.1e-19
Identity = 59/198 (29.80%), Postives = 97/198 (48.99%), Query Frame = 0

Query: 7   EITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKRNDSSD 66
           E  + +  R+T ++ML+DRGY V D ++++S +QF+  YGE    + + I+   R DSS 
Sbjct: 20  ECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRALYGEHPDVDLLRISAKHRFDSSK 79

Query: 67  -----------------------------------------------------------Q 126
                                                                      +
Sbjct: 80  KISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQSHITNQALKAVELFSFKVELFE 139

Query: 127 EAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRGQVV 146
             +LLVN+  HVL P+HQ+L+++EK++LL++++++E QLPR+   DP+ RYYGL+ GQV+
Sbjct: 140 ITDLLVNVSKHVLRPKHQVLNDKEKESLLKKFSIEEKQLPRLSSKDPIVRYYGLETGQVM 199

BLAST of Carg05106 vs. TAIR 10
Match: AT3G57080.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 91.7 bits (226), Expect = 5.4e-19
Identity = 63/202 (31.19%), Postives = 94/202 (46.53%), Query Frame = 0

Query: 3   LAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKRN 62
           L+ EE  R +  R+  LQML+DRGY V D ++++S   F+  YGE    + + I+   R+
Sbjct: 20  LSSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLRISALHRS 79

Query: 63  DSSD-------------------------------------------------------- 122
           DS+                                                         
Sbjct: 80  DSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAIELFSFKV 139

Query: 123 ---QEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLKR 146
              Q  +LLVNI  H L P+HQ+L++EEK TLL++++++E QLPRI   D + RYYGL++
Sbjct: 140 EIFQITDLLVNITKHSLKPQHQVLNDEEKTTLLKKFSIEEKQLPRISKKDAIVRYYGLEK 199

BLAST of Carg05106 vs. TAIR 10
Match: AT3G54490.1 (RNA polymerase II fifth largest subunit, E )

HSP 1 Score: 75.1 bits (183), Expect = 5.2e-14
Identity = 56/199 (28.14%), Postives = 88/199 (44.22%), Query Frame = 0

Query: 7   EITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAKRNDSSD 66
           E  R +  R T  +ML+DRGY V + E+ ++  +F++ +GE  + E + I    R+D   
Sbjct: 35  ESKRFYLARTTAFEMLRDRGYEVNEAELSLTLSEFRSVFGEKPELERLRICVPLRSDPKK 94

Query: 67  ---------------------------------------------QEA------------ 126
                                                        Q+A            
Sbjct: 95  KILVVFMGTEPITVKSVRALHIQISNNVGLHAMILVLQSKMNHFAQKALTTFPFTVETFP 154

Query: 127 --ELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQLPRIQVTDPVARYYGLKRGQVV 147
             +LLVNI  H+  P+ ++L+ EEK+ LL ++ +++ QLP +Q  D   RYYGLK+ QVV
Sbjct: 155 IEDLLVNITKHIQQPKIEILNKEEKEQLLRKHALEDKQLPYLQEKDSFVRYYGLKKKQVV 214

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7025474.11.5e-71100.00DNA-directed RNA polymerases II and IV subunit 5A [Cucurbita argyrosperma subsp.... [more]
XP_022959553.12.6e-6370.87DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_... [more]
ESR64457.13.7e-6284.93hypothetical protein CICLE_v10009504mg [Citrus clementina][more]
KAF3965687.16.3e-6284.25hypothetical protein CMV_010147 [Castanea mollissima][more]
XP_022991826.11.1e-6167.96DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_02... [more]
Match NameE-valueIdentityDescription
O810982.0e-5054.15DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370... [more]
Q9N5K21.0e-3042.51DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis ele... [more]
A8XGH12.3e-3042.03DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Caenorhabditis bri... [more]
Q80UW85.6e-2938.35DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10... [more]
B0BNE25.6e-2938.35DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus ... [more]
Match NameE-valueIdentityDescription
A0A6J1KT451.2e-6370.87DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36... [more]
A0A6J1H6991.2e-6370.87DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=... [more]
V4UEF91.8e-6284.93Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10009504mg PE=3 ... [more]
A0A6J1JRV45.2e-6267.96DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36... [more]
A0A6J1GQK96.8e-6267.48DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=... [more]
Match NameE-valueIdentityDescription
AT3G22320.11.4e-5154.15Eukaryotic rpb5 RNA polymerase subunit family protein [more]
AT5G57980.16.6e-2534.95RNA polymerase II fifth largest subunit, C [more]
AT2G41340.14.1e-1929.80RNA polymerase II fifth largest subunit, D [more]
AT3G57080.15.4e-1931.19Eukaryotic rpb5 RNA polymerase subunit family protein [more]
AT3G54490.15.2e-1428.14RNA polymerase II fifth largest subunit, E [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035913RPB5-like RNA polymerase subunit superfamilyGENE3D3.90.940.20coord: 68..146
e-value: 2.3E-35
score: 122.1
IPR035913RPB5-like RNA polymerase subunit superfamilySUPERFAMILY55287RPB5-like RNA polymerase subunitcoord: 71..146
IPR000783RNA polymerase, subunit H/Rpb5 C-terminalPFAMPF01191RNA_pol_Rpb5_Ccoord: 73..145
e-value: 3.3E-35
score: 119.8
IPR005571RNA polymerase, Rpb5, N-terminalPFAMPF03871RNA_pol_Rpb5_Ncoord: 5..67
e-value: 9.1E-20
score: 71.1
NoneNo IPR availablePANTHERPTHR10535:SF17DNA-DIRECTED RNA POLYMERASES II AND IV SUBUNIT 5A-LIKEcoord: 67..146
coord: 1..67
IPR039531DNA-directed RNA polymerase subunit Rpb5-likePANTHERPTHR10535DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1coord: 67..146
IPR039531DNA-directed RNA polymerase subunit Rpb5-likePANTHERPTHR10535DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1coord: 1..67
IPR020608RNA polymerase, subunit H/Rpb5, conserved sitePROSITEPS01110RNA_POL_H_23KDcoord: 78..91
IPR020609Archaeal RpoH /eukaryotic RPB5 RNA polymerase subunitHAMAPMF_00025RNApol_RpoH_RPB5coord: 73..146
score: 22.870682
IPR036710RNA polymerase Rpb5, N-terminal domain superfamilySUPERFAMILY53036Eukaryotic RPB5 N-terminal domaincoord: 5..67

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg05106-RACarg05106-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006351 transcription, DNA-templated
cellular_component GO:0005736 RNA polymerase I complex
cellular_component GO:0005665 RNA polymerase II, core complex
cellular_component GO:0005666 RNA polymerase III complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0003899 DNA-directed 5'-3' RNA polymerase activity