Carg03557 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03557
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	outer envelope pore protein 16-3, chloroplastic/mitochondrial
Location	Carg_Chr14: 1995591 .. 1999451 (+)
RNA-Seq Expression	Carg03557
Synteny	Carg03557

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGCTTCAGAGCGTAGGTACTTGGAGGATGATGATACGCCAATAATGAAAACGATCAAAGGTGCTACTACAGGATTTGTTTCTGGGACCATTTTTGGGACAATTGCTGCCACTTGGTATGATGTGCCCCGTGTTGAGAGAAATGTTGCTCTGCCCGGGCTTGTGAGAACCTTGAAAATGATGGGAAACTATGGGATGACATTTGCTGCTATTGGAGGGGTATACATTGGCGTTGAGCAGCTTGTGCAGAATTATAGAATGAAAAGAGACTTCGTTAATGGAGCTATAGGTGGTTTTGCAGCTGGAGCATCTGTTCTCGGATATAAAGGTGAAACTTTGTACTCTTTCTAGATCTTGATACGGTCGCAGTTTGAATTCTTAAATTAGCAAACGTGAACAAAGTTCTGTTAACATGTTTTATAAGTGTTGTTTCTCCTTTTCTCTGCTTTCCTGTCATAGAGGATGGAATGAAGTTTTATTTTACGAATGTGGAGAGTTTTTTTTTTCTTTTCTCCTAACTGTAGTTTATGTGGCATGTTTTCATCTGTTGATATTTACATGCTAACTCCAAAAAAGTTAACACCAATTATCATTAATTTAATTCACTCATTATTGTGTTCCGTCTAACCTTTTTGGTTTTCTTACTCTCAACCTCTTTATGAAAAGTGGAACTGGTGCCCTGGAGGGGGATTGTAAGTATGTTATTTAAGCTCGGGTTACTGTAGATTACAGTAGACATATGCTTCAGCAGAAGCGCTCTCCTACTGTGATTTTTGCATCCATCTGGTGTTCTCTTTCTAAGTATTTTTGTAACTATTATGGTGTGCATATTTCAGCTAATTTGGAGTCTTTTTTTTAACACTTTGGTCTAGCATGACTTTCTTGCTATTGATATATTCTTTGTTTCTTGTAAAAAACAAAGAGACAAATTATGAAAGATTACTGTTATTCTGAAGTTCAATACTGGCATGGTTACGGACTAAGTAGCACTATTACATGTGTGAAACTGATACAGAGTACTCTAGACTTTAAGAGGCCTGGAACCACCCCCGCTAATGTACCTTTATTCTAAGGAGTTTTTTTTGACAATATAGAAACAACAAGTTTTCTTTATTATTATTATTATTGTTTAGTATGCCTCACTACTTTTCGTGAGAAAGGTCATGTGTTGTGGTAAGCAAGATTTCTTGCTATTGTTTGGGGTATTTGACTAGAGAGGAACACTAGAATTTTTAGGGAGGTTGGGAGTTCGAATGAGGAGGTCTAGGAGGGGGCTCAATTTAATGCCTCCCCATGGGCGTTTATTAATAACCTCTTTTGTGATTATGATCTTGATTTGATTTTATTAGATTAGAGACATTTTTTAGTTGGTTTGAGGTTCCCTTCACTTTTTTGTGGTTTTTTTTTTTTTTGTTTGTATTCATCTTTGCTATCATTATTTCAAAATGAAACTTTGGTTTCTTAAAAAAAAAGAAGTTTTAAGTATATGTTGCCTTGCATAATGTCAACAAGTCAAAATGCTCATGTGGAAGGATTGCAACTAATTGATTCTATCCATGCTCAGACTGAAGGAATGTAAGATTATATAAATTAGAAAAAGTGGGAATGGATACTTAAGTCTACTACTTTGAGAAAGCCATATAGTTGATTAGGAATTTTTTGCTAATGGCGTTTAAAAGCAAAGGCCTGGGCATGAGATGGATAAAATGGACCAACAAATTCTTGGATCGTGTGATTGCAAACACTAGCTCCCGTTAATGGAATAAAACTTTCTGTTATTGTTCTTAATGGTAGACTAAGAGGGCTGTTGAGGGCTAGATTTAAAAGGAGAGATTTTTTTGTCATTTTCTTTCTTTCTTGATTGAAAATGTGTTGAGTAGTTTGATTGAACATGGTATTTGTGGGGTTTTCTTGAACATTTTTATTCCTTGAGGAGAACCAGGAGCATTTTCATATATTCATTTTGTGGAGAGTAATTTACTTATATGCAAAGATGATAGGAAAGTGTTAGAGAATTTACCCAAGGTACATTGGATTTTTTATATTTATTTGAGACTAAAAATAAATGCGAAAACTTCAGGTTTGTGGTCTAGAATATTCCTTTTGTTGAAGTGAGGGCTTCTGCAAAGAAAATGGGGTGCAACCTTGTAACATGTCAATTTCTTGCTCGGGGATGCCTGTGCATGATAAATTCATGAGGCTAGAGTTTCGGAAGCCCATGGAAGAAGATGAAGTCCTTTATAAGTTTGAAAAATGGAAGTCTTACCTTTCTAATGGGGGAGATTGGCTCTTTGACAAGCAGTTCTTGCCACCTTACCTACCTATTTCATGTCATATTTTCCTGTGTTTAGGTAAATAAAAGTGGTAAGTTTGGAAAAAAAGTTGAGGAATTTTTGTATGGCTGATAATTCAGACAGGAACCCAAGCCATCTTGTACACTGGAATGTGTTGTCTTTGCCTATTGATTGTGGTGGGCTGGGGCTACTTGAGAAAGAAAAAATAGTACTGGTCGCAATGGGTATGCCACTTTTTCAGTGAATCAAATCAAATTTGGCATGAAGTGGTATCTAGCATTTATAGAAGAAAGGAAGGCGAGTGTTTACCTATTTAGACTTCATTGTCCACGATATCTAAAAATGGAAGAACCATTCAACCTGCATACCTCTAGCCGGTAGCCCAATTCAGAATAACTTAGATCATGGTAAAAGTTCAACATTGAACGCCTATTAGAATCATTCCATAATTGCTAGCAAGCATAGAGTCTAGTAGTTAAAAGGAGAGAGTAGTCTTGTCCAGATGTCATTCCAAAATTTAACTCTACTGCCTTCTCCCACCTAAAACATGCAACCAAAGGCTGCAGTATCCAAAATATATCTAAGCTTGGAAATATGCAAGCAACTGCTGCAGGACATTTCTGCACCTGGTGATACACCTGACTTATCCTTCCCATACCTACCATGGTGCATTACTTTCTAAAAGGAACCACAATATGCATTTGGTTAAAAATGTGTCGTTTCACTTAGCTATATTTATGACGCCAAGACCTCTGTGTTCCAAAGAGCGAGAGACCTCTGTGTACTTTATTAATAGGCAGCCTCCATCCTAGTCTGCACCTCCCTAAAGAATTCTCCTCTCCTATGTTTATCAAAGTTTCTACCTTAAAGACAAATAACTAGTCTGCATCTTAGTACCAACCTCTTAGGCAGGACAGAGGCTTAAGTTTGGACTTGGACTGCAAAAGTTTGCATTCCAACTAAATTAAATGGGGCAAATTGGAGGGAAGTGCTATTTAATACACACACACACACACAAAATCTAAGACAAAGGAGGGAGGGAGAGAATTTCAAACACTCTTGTAATACCTACTTTCTCCGCTAAAGCTATGACCTTTTCTTCATAATTGTTTTTAATATTGCTAGACCCCATGTCACTCTGAATTAATCCCTACTTGGCTGAATGAATGAAAGTCGTATCCACACTATGTGGTACTCGAAATTGTCTCACAAGAGGTGGCAATTGCCTGATATGGGTATAGCTTGATTGTTATATTTGTCACAATAATCCCTATCAGCACATTTTTGTAAAGTGAAGTGTCGTTCTCATCTTTCTGATCCGTTGCTTTAGGACGTGGTTCTAATCTGTTTTCTTTTCATGTCCCTAATAAGTAAACTTGGTCTGAAAGTGGTTAACTGTTGCTATTGTTTTCTTTCAGGAAAAAGTATTCCAACAGCCATTTCTGCTGGAGCTGCTTTGGCAGTTACTTCTGCACTAATTGATGTTGGTGGTCAGACCACAAGAATTGACAATGGCAAGGAGTACTACCCTTACACCACCAAGAAAAGATCCCACATCGAGTAG

mRNA sequence

ATGGATGCTTCAGAGCGTAGGTACTTGGAGGATGATGATACGCCAATAATGAAAACGATCAAAGGTGCTACTACAGGATTTGTTTCTGGGACCATTTTTGGGACAATTGCTGCCACTTGGTATGATGTGCCCCGTGTTGAGAGAAATGTTGCTCTGCCCGGGCTTGTGAGAACCTTGAAAATGATGGGAAACTATGGGATGACATTTGCTGCTATTGGAGGGGTATACATTGGCGTTGAGCAGCTTGTGCAGAATTATAGAATGAAAAGAGACTTCGTTAATGGAGCTATAGGTGGTTTTGCAGCTGGAGCATCTGTTCTCGGATATAAAGGAAAAAGTATTCCAACAGCCATTTCTGCTGGAGCTGCTTTGGCAGTTACTTCTGCACTAATTGATGTTGGTGGTCAGACCACAAGAATTGACAATGGCAAGGAGTACTACCCTTACACCACCAAGAAAAGATCCCACATCGAGTAG

Coding sequence (CDS)

ATGGATGCTTCAGAGCGTAGGTACTTGGAGGATGATGATACGCCAATAATGAAAACGATCAAAGGTGCTACTACAGGATTTGTTTCTGGGACCATTTTTGGGACAATTGCTGCCACTTGGTATGATGTGCCCCGTGTTGAGAGAAATGTTGCTCTGCCCGGGCTTGTGAGAACCTTGAAAATGATGGGAAACTATGGGATGACATTTGCTGCTATTGGAGGGGTATACATTGGCGTTGAGCAGCTTGTGCAGAATTATAGAATGAAAAGAGACTTCGTTAATGGAGCTATAGGTGGTTTTGCAGCTGGAGCATCTGTTCTCGGATATAAAGGAAAAAGTATTCCAACAGCCATTTCTGCTGGAGCTGCTTTGGCAGTTACTTCTGCACTAATTGATGTTGGTGGTCAGACCACAAGAATTGACAATGGCAAGGAGTACTACCCTTACACCACCAAGAAAAGATCCCACATCGAGTAG

Protein sequence

MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLKMMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISAGAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE

Homology

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BLAST of Carg03557 vs. NCBI nr
Match: XP_022935378.1 (outer envelope pore protein 16-3, chloroplastic/mitochondrial [Cucurbita moschata] >KAG7017569.1 Outer envelope pore protein 16-3, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 314.3 bits (804), Expect = 5.9e-82
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of Carg03557 vs. NCBI nr
Match: XP_022983139.1 (outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita maxima] >XP_023528706.1 outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 311.2 bits (796), Expect = 5.0e-81
Identity = 157/158 (99.37%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDT IMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of Carg03557 vs. NCBI nr
Match: XP_022971491.1 (outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 306.2 bits (783), Expect = 1.6e-79
Identity = 154/158 (97.47%), Postives = 155/158 (98.10%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDT IMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGA+GGFAAGASVLGYKGKSI TAISA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTAISA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           G ALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE
Sbjct: 121 GVALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of Carg03557 vs. NCBI nr
Match: XP_022948275.1 (outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita moschata] >XP_023540815.1 outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 305.4 bits (781), Expect = 2.8e-79
Identity = 154/158 (97.47%), Postives = 155/158 (98.10%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDT IMK IKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSIMKMIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGA+GGFAAGASVLGYKGKSI TAISA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTAISA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of Carg03557 vs. NCBI nr
Match: KAG7027770.1 (Outer envelope pore protein 16-3, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 305.4 bits (781), Expect = 2.8e-79
Identity = 154/158 (97.47%), Postives = 155/158 (98.10%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDT IMK IKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK
Sbjct: 42  MDASERRYLEDDDTSIMKMIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 101

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGA+GGFAAGASVLGYKGKSI TAISA
Sbjct: 102 MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTAISA 161

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE
Sbjct: 162 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 199

[Close]

BLAST of Carg03557 vs. ExPASy Swiss-Prot
Match: O48528 (Outer envelope pore protein 16-3, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OEP163 PE=1 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 1.6e-66
Identity = 122/158 (77.22%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MD +E RYLE++D P+MKTIKG+ TGF +GTI+GTI ATW DVPRVERNVALPGL+RTLK
Sbjct: 1   MDPAEMRYLEEEDGPLMKTIKGSITGFGAGTIYGTILATWKDVPRVERNVALPGLIRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMG +G+TFAAIGGVYIGVEQLVQN+R KRDF NGAIGGF AGASVLGY+ +SIPTAI+A
Sbjct: 61  MMGTHGLTFAAIGGVYIGVEQLVQNFRSKRDFYNGAIGGFVAGASVLGYRARSIPTAIAA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GA LAVTSALID GGQTTR+DNG+EYYPYT +KR+  +
Sbjct: 121 GATLAVTSALIDSGGQTTRVDNGREYYPYTVEKRAEAD 158

[Close]

BLAST of Carg03557 vs. ExPASy Swiss-Prot
Match: P0CR89 (Mitochondrial import inner membrane translocase subunit TIM22 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=TIM22 PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.4e-04
Identity = 44/131 (33.59%), Postives = 58/131 (44.27%), Query Frame = 0


Query: 12  DDTPIMKTIKGATTGFVSGTIFGTIAATW-YDVPRVERNVAL----PGLVRTLKMMG--- 71
           +  P+  TI G   G   G  F  ++AT+ Y+ P    +  L       +   K MG   
Sbjct: 50  ESCPLKVTIAG-VGGLAIGGFFSLMSATFAYEDPLSRASNKLTTTRAQTMFVFKEMGRNM 109

Query: 72  -NYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTA-ISAG 131
            + G  FA +G VY GVE  ++ YR K D  NG   GF  GA +    G   PTA +  G
Sbjct: 110 WSSGRGFAKVGMVYSGVECCIEGYRAKNDIYNGVSAGFLTGAILARNAG---PTAMLGGG 169

Query: 132 AALAVTSALID 133
            A A  S  ID
Sbjct: 170 VAFAAFSGAID 176

[Close]

BLAST of Carg03557 vs. ExPASy Swiss-Prot
Match: P0CR88 (Mitochondrial import inner membrane translocase subunit TIM22 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=TIM22 PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.4e-04
Identity = 44/131 (33.59%), Postives = 58/131 (44.27%), Query Frame = 0


Query: 12  DDTPIMKTIKGATTGFVSGTIFGTIAATW-YDVPRVERNVAL----PGLVRTLKMMG--- 71
           +  P+  TI G   G   G  F  ++AT+ Y+ P    +  L       +   K MG   
Sbjct: 50  ESCPLKVTIAG-VGGLAIGGFFSLMSATFAYEDPLSRASNKLTTTRAQTMFVFKEMGRNM 109

Query: 72  -NYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTA-ISAG 131
            + G  FA +G VY GVE  ++ YR K D  NG   GF  GA +    G   PTA +  G
Sbjct: 110 WSSGRGFAKVGMVYSGVECCIEGYRAKNDIYNGVSAGFLTGAILARNAG---PTAMLGGG 169

Query: 132 AALAVTSALID 133
            A A  S  ID
Sbjct: 170 VAFAAFSGAID 176

[Close]

BLAST of Carg03557 vs. ExPASy Swiss-Prot
Match: Q9ZV24 (Outer envelope pore protein 16-1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=OEP161 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 5.2e-04
Identity = 31/81 (38.27%), Postives = 42/81 (51.85%), Query Frame = 0


Query: 55  LVRTLKMMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGA--SVLGYKGK 114
           L   LK +   G+ + A GGVYIG E  ++  R  RD+ N  + G A GA  S +G KGK
Sbjct: 63  LEHALKKLCKEGVYWGAAGGVYIGTEYGIERIRGSRDWKNAMLAGAATGAVLSAVGKKGK 122

Query: 115 -SIPTAISAGAALAVTSALID 133
            +I      G ALA  S  ++
Sbjct: 123 DTIVIDAILGGALATASQFVN 143

[Close]

BLAST of Carg03557 vs. ExPASy TrEMBL
Match: A0A6J1FAE0 (outer envelope pore protein 16-3, chloroplastic/mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111442280 PE=4 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 2.9e-82
Identity = 158/158 (100.00%), Postives = 158/158 (100.00%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of Carg03557 vs. ExPASy TrEMBL
Match: A0A6J1J6G7 (outer envelope pore protein 16-3, chloroplastic/mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111481780 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 2.4e-81
Identity = 157/158 (99.37%), Postives = 157/158 (99.37%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDT IMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of Carg03557 vs. ExPASy TrEMBL
Match: A0A6J1I3G7 (outer envelope pore protein 16-3, chloroplastic/mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111470193 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 7.8e-80
Identity = 154/158 (97.47%), Postives = 155/158 (98.10%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDT IMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGA+GGFAAGASVLGYKGKSI TAISA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTAISA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           G ALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE
Sbjct: 121 GVALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of Carg03557 vs. ExPASy TrEMBL
Match: A0A6J1G9D0 (outer envelope pore protein 16-3, chloroplastic/mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111451994 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.3e-79
Identity = 154/158 (97.47%), Postives = 155/158 (98.10%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDT IMK IKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK
Sbjct: 1   MDASERRYLEDDDTSIMKMIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGA+GGFAAGASVLGYKGKSI TAISA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTAISA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of Carg03557 vs. ExPASy TrEMBL
Match: A0A6J1DN96 (outer envelope pore protein 16-3, chloroplastic/mitochondrial OS=Momordica charantia OX=3673 GN=LOC111021571 PE=4 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.5e-78
Identity = 150/158 (94.94%), Postives = 154/158 (97.47%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MDASERRYLEDDDT +MKTIKGATTGFV+GTIFGTIAATWYDVPRVERNVALPG +RTLK
Sbjct: 1   MDASERRYLEDDDTSMMKTIKGATTGFVAGTIFGTIAATWYDVPRVERNVALPGFLRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGA+GGFAAGASVLGYKGKSI TAISA
Sbjct: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAVGGFAAGASVLGYKGKSISTAISA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GAALAVTS LIDVGGQTTRIDNGKEYYPYTTKKRSHIE
Sbjct: 121 GAALAVTSTLIDVGGQTTRIDNGKEYYPYTTKKRSHIE 158

[Close]

BLAST of Carg03557 vs. TAIR 10
Match: AT2G42210.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 253.4 bits (646), Expect = 1.2e-67
Identity = 122/158 (77.22%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MD +E RYLE++D P+MKTIKG+ TGF +GTI+GTI ATW DVPRVERNVALPGL+RTLK
Sbjct: 1   MDPAEMRYLEEEDGPLMKTIKGSITGFGAGTIYGTILATWKDVPRVERNVALPGLIRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMG +G+TFAAIGGVYIGVEQLVQN+R KRDF NGAIGGF AGASVLGY+ +SIPTAI+A
Sbjct: 61  MMGTHGLTFAAIGGVYIGVEQLVQNFRSKRDFYNGAIGGFVAGASVLGYRARSIPTAIAA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GA LAVTSALID GGQTTR+DNG+EYYPYT +KR+  +
Sbjct: 121 GATLAVTSALIDSGGQTTRVDNGREYYPYTVEKRAEAD 158

[Close]

BLAST of Carg03557 vs. TAIR 10
Match: AT2G42210.2 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 253.4 bits (646), Expect = 1.2e-67
Identity = 122/158 (77.22%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MD +E RYLE++D P+MKTIKG+ TGF +GTI+GTI ATW DVPRVERNVALPGL+RTLK
Sbjct: 15  MDPAEMRYLEEEDGPLMKTIKGSITGFGAGTIYGTILATWKDVPRVERNVALPGLIRTLK 74

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMG +G+TFAAIGGVYIGVEQLVQN+R KRDF NGAIGGF AGASVLGY+ +SIPTAI+A
Sbjct: 75  MMGTHGLTFAAIGGVYIGVEQLVQNFRSKRDFYNGAIGGFVAGASVLGYRARSIPTAIAA 134

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GA LAVTSALID GGQTTR+DNG+EYYPYT +KR+  +
Sbjct: 135 GATLAVTSALIDSGGQTTRVDNGREYYPYTVEKRAEAD 172

[Close]

BLAST of Carg03557 vs. TAIR 10
Match: AT2G42210.3 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 253.4 bits (646), Expect = 1.2e-67
Identity = 122/158 (77.22%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MD +E RYLE++D P+MKTIKG+ TGF +GTI+GTI ATW DVPRVERNVALPGL+RTLK
Sbjct: 1   MDPAEMRYLEEEDGPLMKTIKGSITGFGAGTIYGTILATWKDVPRVERNVALPGLIRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMG +G+TFAAIGGVYIGVEQLVQN+R KRDF NGAIGGF AGASVLGY+ +SIPTAI+A
Sbjct: 61  MMGTHGLTFAAIGGVYIGVEQLVQNFRSKRDFYNGAIGGFVAGASVLGYRARSIPTAIAA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GA LAVTSALID GGQTTR+DNG+EYYPYT +KR+  +
Sbjct: 121 GATLAVTSALIDSGGQTTRVDNGREYYPYTVEKRAEAD 158

[Close]

BLAST of Carg03557 vs. TAIR 10
Match: AT2G42210.4 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 253.4 bits (646), Expect = 1.2e-67
Identity = 122/158 (77.22%), Postives = 141/158 (89.24%), Query Frame = 0


Query: 1   MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
           MD +E RYLE++D P+MKTIKG+ TGF +GTI+GTI ATW DVPRVERNVALPGL+RTLK
Sbjct: 1   MDPAEMRYLEEEDGPLMKTIKGSITGFGAGTIYGTILATWKDVPRVERNVALPGLIRTLK 60

Query: 61  MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGAIGGFAAGASVLGYKGKSIPTAISA 120
           MMG +G+TFAAIGGVYIGVEQLVQN+R KRDF NGAIGGF AGASVLGY+ +SIPTAI+A
Sbjct: 61  MMGTHGLTFAAIGGVYIGVEQLVQNFRSKRDFYNGAIGGFVAGASVLGYRARSIPTAIAA 120

Query: 121 GAALAVTSALIDVGGQTTRIDNGKEYYPYTTKKRSHIE 159
           GA LAVTSALID GGQTTR+DNG+EYYPYT +KR+  +
Sbjct: 121 GATLAVTSALIDSGGQTTRVDNGREYYPYTVEKRAEAD 158

[Close]

BLAST of Carg03557 vs. TAIR 10
Match: AT3G57900.1 (unknown protein; Has 22 Blast hits to 22 proteins in 7 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 22; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 52.8 bits (125), Expect = 3.0e-07
Identity = 34/96 (35.42%), Postives = 42/96 (43.75%), Query Frame = 0


Query: 1  MDASERRYLEDDDTPIMKTIKGATTGFVSGTIFGTIAATWYDVPRVERNVALPGLVRTLK 60
          MD +E RYLE+++  +MKT K A TGF +G                              
Sbjct: 1  MDPTEMRYLEEENGAMMKTFKDAVTGFATG------------------------------ 56

Query: 61 MMGNYGMTFAAIGGVYIGVEQLVQNYRMKRDFVNGA 97
                        V+IGVEQLVQN+R KRDF NGA
Sbjct: 61 ----------KTSLVFIGVEQLVQNFRAKRDFFNGA 56

The following BLAST results are available for this feature:

BLAST of Carg03557 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022935378.1	5.9e-82	100.00	outer envelope pore protein 16-3, chloroplastic/mitochondrial [Cucurbita moschat...	[more]
XP_022983139.1	5.0e-81	99.37	outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita ma...	[more]
XP_022971491.1	1.6e-79	97.47	outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita ma...	[more]
XP_022948275.1	2.8e-79	97.47	outer envelope pore protein 16-3, chloroplastic/mitochondrial-like [Cucurbita mo...	[more]
KAG7027770.1	2.8e-79	97.47	Outer envelope pore protein 16-3, chloroplastic/mitochondrial, partial [Cucurbit...	[more]

BLAST of Carg03557 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48528	1.6e-66	77.22	Outer envelope pore protein 16-3, chloroplastic/mitochondrial OS=Arabidopsis tha...	[more]
P0CR89	1.4e-04	33.59	Mitochondrial import inner membrane translocase subunit TIM22 OS=Cryptococcus ne...	[more]
P0CR88	1.4e-04	33.59	Mitochondrial import inner membrane translocase subunit TIM22 OS=Cryptococcus ne...	[more]
Q9ZV24	5.2e-04	38.27	Outer envelope pore protein 16-1, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Carg03557 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FAE0	2.9e-82	100.00	outer envelope pore protein 16-3, chloroplastic/mitochondrial OS=Cucurbita mosch...	[more]
A0A6J1J6G7	2.4e-81	99.37	outer envelope pore protein 16-3, chloroplastic/mitochondrial-like OS=Cucurbita ...	[more]
A0A6J1I3G7	7.8e-80	97.47	outer envelope pore protein 16-3, chloroplastic/mitochondrial-like OS=Cucurbita ...	[more]
A0A6J1G9D0	1.3e-79	97.47	outer envelope pore protein 16-3, chloroplastic/mitochondrial-like OS=Cucurbita ...	[more]
A0A6J1DN96	2.5e-78	94.94	outer envelope pore protein 16-3, chloroplastic/mitochondrial OS=Momordica chara...	[more]

BLAST of Carg03557 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G42210.1	1.2e-67	77.22	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT2G42210.2	1.2e-67	77.22	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT2G42210.3	1.2e-67	77.22	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT2G42210.4	1.2e-67	77.22	Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family...	[more]
AT3G57900.1	3.0e-07	35.42	unknown protein; Has 22 Blast hits to 22 proteins in 7 species: Archae - 0; Bact...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF02466	Tim17	coord: 18..128 e-value: 5.8E-14 score: 52.5
None	No IPR available	PANTHER	PTHR14110:SF18	OUTER ENVELOPE PORE PROTEIN 16-3, CHLOROPLASTIC/MITOCHONDRIAL	coord: 3..156
IPR039175	Mitochondrial import inner membrane translocase subunit TIM22	PANTHER	PTHR14110	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM22	coord: 3..156

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03557-RA	Carg03557-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045039	protein insertion into mitochondrial inner membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0042721	TIM22 mitochondrial import inner membrane insertion complex