Carg03251 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg03251
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Proteasome subunit beta
Location	Carg_Chr20: 2334603 .. 2337160 (-)
RNA-Seq Expression	Carg03251
Synteny	Carg03251

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGACGACGTTTAGGGCCACTGCTGGGGAAAGAGAACGATCTGAACAAGAAAGATCATTTCTTGAGCGAAGGGAAACGAAGAGCTCTACTAAAGCAGTCGAAAGGCTCCAACTTCGCTGCGACGCTCGACATTGATTCTGTTCCCAATCCATTGTGCCGAAGCTAATCGATCCGAATATGAGTGTAAGTTCATCATCTGGACCTCAAATTTATATTCCTCATTACATTCCGATTCTAACATTCATCGATCTTCCGATTTTTGTATGCAGCCCGTTGATTCTAATCAGGCGAACCACGGTCTTCTCTGCCCCGAAGCTCCAATTCAAAGAACTCAGTACGATCTCTCTCTCTCTCTGTTTGTTTCTGTGGTTACTTTTTGGTGTTAGACTATACGTTTCGTATTCTAGAGCTGGGAATTTTAGGGTTCTAAAGACCTAAGATTCGAATTTAGAGTTAAGCAGTGAATGAATGGACAAATCGAGGTATTAAAATCGACTACTTCTTCTGTCTAATAGGTATCCCTATGTGACTGGTACATCCGTGGTTGCCATCAAATACAAAGATGGGATTTTAATGGTTTCTGATTTGGGAGGTCAGTCGAAAATCTTCTCCATTTTTATATTTCAACATCCAGTGAACTGTTCATTTTATTATGTTTCTCAGCATGTTTATCACTTTTTATTTGCTGATAACAAAACACTTCTGGAAGTGATGTTTCTTTCTTTATGTGATTTCGAATCTCTGATGTATGTATTGCCAAATTATAAGGCAAACCCGTCTGTATTACTTATATGGCGCTTTAACTAACTACAGGCTCCTATGGTTCCACACTGCGGTATAAGAGTGTGGAGCGAATCAAAGCTATTGGAAAACATTCTCTACTTGGAGCAAGCGGAGAAATTAGTGACTTTCAGGAGTTATTGAGAAATCTTGATGAGCTTATGTAAGCCTAAGATGTTTGCTTTATTCTATCTCTTTGATTTTGATGCTGTTCTGCATTGGAATTCAGTTGCTTGTTTCGTGCGTCTATTTTGATTTAGAACTTGAGTGAAATCTCTAATTGTATCGAATGGTGCAGCTTGTATGATAACATGTGGGATGATGGTAATGCTTTGGGGCCAAAGGAGATCCATAGCTACTTAACTCGAGTGATGTATAACAGGCGGAACAAGTTCAACCCACTTTGGAACTCGCTTGTTCTCGGTGGGGTGAAGAACGGGCAAAAGTATCTTGGCACGGTAGATCTTTGGTTCTCTTCGCATTCATTAAAATAAGTTCCTAATATCCGTTCTAAAATAAGATGCTTCATCAGCTAACATTTAGCCACTTTTATTCAGGTTAATATGATAGGTGTAACTTTTGAAGATGATCATGTTACTACTGGCTTTGGCAATCACCTTGCACGACCAATTCTTCGTGATGAGTGGCACGAGAACCTGACTTTTGAAGAAGGTGTGAAATTGCTGGAGAAATGCATGAGGGTTCTCTTATACCGTGATAGATCGGCTGTCAACAAGCTCCAGGTTTGGATTTTTCTATGAGCTACCTTCATTGCTTTCTTTGCTATATATTTTGTGAGCGTGTGATTCTCGTCTGCGCGTATCGACAAATCTCATAAAATAGCCATCCAACACTAACATTTGATCTTCATAGAAACTCATTAGATTTTAAGATCATAAAGAGTTAGATCACTATGTGTTTCAGCCCATTCTAATAGCTACTTTCTAGTTTCTGGAACACATGTTTTGTTCACTGATTATAAGACTTCAATGTCTGGATGTTATTCTCATCATCACATACATTTGAATATGAAATTTACAGATTGCAAAAATAACTGAAGAAGGTGTGACAATCTCCCAACCCTACTCTTTGAAAACATTCTGGAACTACTCTGCTTTCGAGAATCCGACTTCGGGTGCTGAAGGGTCATGGTAGATTGAAGCTTCGTCGTTTATCCGAGATTCCATTTGGGTGAGTCGCAGTTTGGGGCGCAACAATTGATAGATCTAAGTTAGATGCATCTTAGATACTTCGCTCTTTGGATTTGTATGGAATCTCTACTTGAACTATCAACTATATCCAACTTGGGGAATGTTTGATTTGATTTTCTTGATATTATTATGATCACAACCGGTTGCTTTTCCATTATGAATCTGCCTACTCAATTGCTTGAACCCTTTGATTCACATGAAGTAAAGTTCAATCTAGTTTGTTGTGGGAAAAGCTCTGATTTGTGAGGTTGAAAGTTGAAACTAAATAGATCGAGACGTTATGCTGATACTTCGAAAGGAATTTGGATATCGACACTAATCCAAGTCCTAAACTACAATATGAGTAAACGACAGGTAAGAGTTCAGCTAGCATAATACAATAAGACATCAAATACTAAATCGAAAGTTTAAGTGAAAAAGAGATCTAATATTTTTGTTAACTAACATTACGAACATTCTCGTACCTCTAAAGGAGAAGATAGATGAGCTCGGGGCACCATTCTTCGCATTGTATATTCAAATCTTCGGCTTGCTCCGTAGAAGATATGATGCCGACACTGGAACTCGG

mRNA sequence

CGACGACGTTTAGGGCCACTGCTGGGGAAAGAGAACGATCTGAACAAGAAAGATCATTTCTTGAGCGAAGGGAAACGAAGAGCTCTACTAAAGCAGTCGAAAGGCTCCAACTTCGCTGCGACGCTCGACATTGATTCTGTTCCCAATCCATTGTGCCGAAGCTAATCGATCCGAATATGAGTCCCGTTGATTCTAATCAGGCGAACCACGGTCTTCTCTGCCCCGAAGCTCCAATTCAAAGAACTCAGTATCCCTATGTGACTGGTACATCCGTGGTTGCCATCAAATACAAAGATGGGATTTTAATGGTTTCTGATTTGGGAGGCTCCTATGGTTCCACACTGCGGTATAAGAGTGTGGAGCGAATCAAAGCTATTGGAAAACATTCTCTACTTGGAGCAAGCGGAGAAATTAGTGACTTTCAGGAGTTATTGAGAAATCTTGATGAGCTTATCTTGTATGATAACATGTGGGATGATGGTAATGCTTTGGGGCCAAAGGAGATCCATAGCTACTTAACTCGAGTGATGTATAACAGGCGGAACAAGTTCAACCCACTTTGGAACTCGCTTGTTCTCGGTGGGGTGAAGAACGGGCAAAAGTATCTTGGCACGGTTAATATGATAGGTGTAACTTTTGAAGATGATCATGTTACTACTGGCTTTGGCAATCACCTTGCACGACCAATTCTTCGTGATGAGTGGCACGAGAACCTGACTTTTGAAGAAGGTGTGAAATTGCTGGAGAAATGCATGAGGGTTCTCTTATACCGTGATAGATCGGCTGTCAACAAGCTCCAGATTGCAAAAATAACTGAAGAAGGTGTGACAATCTCCCAACCCTACTCTTTGAAAACATTCTGGAACTACTCTGCTTTCGAGAATCCGACTTCGGGTGCTGAAGGGTCATGGTAGATTGAAGCTTCGTCGTTTATCCGAGATTCCATTTGGGTGAGTCGCAGTTTGGGGCGCAACAATTGATAGATCTAAGTTAGATGCATCTTAGATACTTCGCTCTTTGGATTTGTATGGAATCTCTACTTGAACTATCAACTATATCCAACTTGGGGAATGTTTGATTTGATTTTCTTGATATTATTATGATCACAACCGGTTGCTTTTCCATTATGAATCTGCCTACTCAATTGCTTGAACCCTTTGATTCACATGAAGTAAAGTTCAATCTAGTTTGTTGTGGGAAAAGCTCTGATTTGTGAGGTTGAAAGTTGAAACTAAATAGATCGAGACGTTATGCTGATACTTCGAAAGGAATTTGGATATCGACACTAATCCAAGTCCTAAACTACAATATGAGTAAACGACAGGTAAGAGTTCAGCTAGCATAATACAATAAGACATCAAATACTAAATCGAAAGTTTAAGTGAAAAAGAGATCTAATATTTTTGTTAACTAACATTACGAACATTCTCGTACCTCTAAAGGAGAAGATAGATGAGCTCGGGGCACCATTCTTCGCATTGTATATTCAAATCTTCGGCTTGCTCCGTAGAAGATATGATGCCGACACTGGAACTCGG

Coding sequence (CDS)

ATGAGTCCCGTTGATTCTAATCAGGCGAACCACGGTCTTCTCTGCCCCGAAGCTCCAATTCAAAGAACTCAGTATCCCTATGTGACTGGTACATCCGTGGTTGCCATCAAATACAAAGATGGGATTTTAATGGTTTCTGATTTGGGAGGCTCCTATGGTTCCACACTGCGGTATAAGAGTGTGGAGCGAATCAAAGCTATTGGAAAACATTCTCTACTTGGAGCAAGCGGAGAAATTAGTGACTTTCAGGAGTTATTGAGAAATCTTGATGAGCTTATCTTGTATGATAACATGTGGGATGATGGTAATGCTTTGGGGCCAAAGGAGATCCATAGCTACTTAACTCGAGTGATGTATAACAGGCGGAACAAGTTCAACCCACTTTGGAACTCGCTTGTTCTCGGTGGGGTGAAGAACGGGCAAAAGTATCTTGGCACGGTTAATATGATAGGTGTAACTTTTGAAGATGATCATGTTACTACTGGCTTTGGCAATCACCTTGCACGACCAATTCTTCGTGATGAGTGGCACGAGAACCTGACTTTTGAAGAAGGTGTGAAATTGCTGGAGAAATGCATGAGGGTTCTCTTATACCGTGATAGATCGGCTGTCAACAAGCTCCAGATTGCAAAAATAACTGAAGAAGGTGTGACAATCTCCCAACCCTACTCTTTGAAAACATTCTGGAACTACTCTGCTTTCGAGAATCCGACTTCGGGTGCTGAAGGGTCATGGTAG

Protein sequence

MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSVERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYNRRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENLTFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSGAEGSW

Homology

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BLAST of Carg03251 vs. NCBI nr
Match: KAG7010616.1 (Proteasome subunit beta type-4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506.9 bits (1304), Expect = 9.7e-140
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60
           MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS
Sbjct: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60

Query: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120
           VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN
Sbjct: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120

Query: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180
           RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL
Sbjct: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180

Query: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240
           TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG
Sbjct: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240

Query: 241 AEGSW 246
           AEGSW
Sbjct: 241 AEGSW 245

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BLAST of Carg03251 vs. NCBI nr
Match: XP_023512002.1 (proteasome subunit beta type-4-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 505.8 bits (1301), Expect = 2.2e-139
Identity = 244/245 (99.59%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60
           MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS
Sbjct: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60

Query: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120
           VERIKAIGKHSLLGASGEISDFQELLRNLD+LILYDNMWDDGNALGPKEIHSYLTRVMYN
Sbjct: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDDLILYDNMWDDGNALGPKEIHSYLTRVMYN 120

Query: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180
           RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL
Sbjct: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180

Query: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240
           TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG
Sbjct: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240

Query: 241 AEGSW 246
           AEGSW
Sbjct: 241 AEGSW 245

[Close]

BLAST of Carg03251 vs. NCBI nr
Match: XP_022986112.1 (proteasome subunit beta type-4-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 504.2 bits (1297), Expect = 6.3e-139
Identity = 244/245 (99.59%), Postives = 244/245 (99.59%), Query Frame = 0


Query: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60
           MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS
Sbjct: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60

Query: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120
           VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN
Sbjct: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120

Query: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180
           RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL
Sbjct: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180

Query: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240
           TF EGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG
Sbjct: 181 TFVEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240

Query: 241 AEGSW 246
           AEGSW
Sbjct: 241 AEGSW 245

[Close]

BLAST of Carg03251 vs. NCBI nr
Match: XP_022948016.1 (proteasome subunit beta type-4-like isoform X1 [Cucurbita moschata] >XP_023532284.1 proteasome subunit beta type-4-like [Cucurbita pepo subsp. pepo] >KAG7035465.1 Proteasome subunit beta type-4 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 503.8 bits (1296), Expect = 8.2e-139
Identity = 242/245 (98.78%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60
           MSPVDSNQANHG+LCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS
Sbjct: 1   MSPVDSNQANHGILCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60

Query: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120
           VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN
Sbjct: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120

Query: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180
           RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL
Sbjct: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180

Query: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240
           T+EEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNY+AFENPTSG
Sbjct: 181 TYEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYAAFENPTSG 240

Query: 241 AEGSW 246
           AEGSW
Sbjct: 241 AEGSW 245

[Close]

BLAST of Carg03251 vs. NCBI nr
Match: XP_022944642.1 (proteasome subunit beta type-4-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 503.8 bits (1296), Expect = 8.2e-139
Identity = 244/245 (99.59%), Postives = 244/245 (99.59%), Query Frame = 0


Query: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60
           MS VDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS
Sbjct: 1   MSSVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60

Query: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120
           VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN
Sbjct: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120

Query: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180
           RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL
Sbjct: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180

Query: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240
           TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG
Sbjct: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240

Query: 241 AEGSW 246
           AEGSW
Sbjct: 241 AEGSW 245

[Close]

BLAST of Carg03251 vs. ExPASy Swiss-Prot
Match: Q7DLR9 (Proteasome subunit beta type-4 OS=Arabidopsis thaliana OX=3702 GN=PBG1 PE=1 SV=2)

HSP 1 Score: 415.2 bits (1066), Expect = 5.0e-115
Identity = 194/243 (79.84%), Postives = 220/243 (90.53%), Query Frame = 0


Query: 3   PVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSVE 62
           P+D+  +   +   E   QRT YPYVTGTS+VAIKYKDG+LM SD+GGSYGSTLRYK++E
Sbjct: 7   PIDNGDS---MKIAEEESQRTLYPYVTGTSIVAIKYKDGVLMASDMGGSYGSTLRYKNIE 66

Query: 63  RIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYNRR 122
           R+KAIGKHSLLGASGEISDFQE+LR LDEL L DNMWDDGN+LGPKEIH+YLTRVMYNRR
Sbjct: 67  RVKAIGKHSLLGASGEISDFQEILRYLDELTLNDNMWDDGNSLGPKEIHNYLTRVMYNRR 126

Query: 123 NKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENLTF 182
           NKFNPLWN+LVLGGVKNG+ YLG V+MIGV+FEDDHV TGFGNHLARPILRDEWH +L+F
Sbjct: 127 NKFNPLWNTLVLGGVKNGKSYLGMVSMIGVSFEDDHVATGFGNHLARPILRDEWHADLSF 186

Query: 183 EEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSGAE 242
           E+GVKLLEKCMRVLLYRDRSA+NKLQIAKITEEGVT+SQPYSLKT+W +S+F NPT+GA 
Sbjct: 187 EDGVKLLEKCMRVLLYRDRSAINKLQIAKITEEGVTVSQPYSLKTYWEFSSFHNPTAGAA 246

Query: 243 GSW 246
           GSW
Sbjct: 247 GSW 246

[Close]

BLAST of Carg03251 vs. ExPASy Swiss-Prot
Match: Q3T108 (Proteasome subunit beta type-4 OS=Bos taurus OX=9913 GN=PSMB4 PE=1 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.3e-54
Identity = 103/231 (44.59%), Postives = 160/231 (69.26%), Query Frame = 0


Query: 2   SPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSV 61
           S VD   A +G     +PI RTQ P VTGTSV+ +K++ G+++ +D+ GSYGS  R++++
Sbjct: 30  SSVDPVSALYG-----SPITRTQNPMVTGTSVLGLKFEGGVVIAADMLGSYGSLARFRNI 89

Query: 62  ERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYNR 121
            RI  +   ++LGASG+ +DFQ L + L ++++ + +  DG++  PK IHS+LTR MY+R
Sbjct: 90  SRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPKAIHSWLTRAMYSR 149

Query: 122 RNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRD--EWHEN 181
           R+K NPLWN++V+GG  +G+ +LG V+M+GV +E   + TG+G +LA+P+LR+  E    
Sbjct: 150 RSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPV 209

Query: 182 LTFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWN 231
           L+  E  +L+E+CMRVL YRD  + N+ QIA +TE+GV I  P S +T W+
Sbjct: 210 LSQTEARELVERCMRVLYYRDARSYNRFQIATVTEKGVEIEGPLSAETNWD 255

[Close]

BLAST of Carg03251 vs. ExPASy Swiss-Prot
Match: P99026 (Proteasome subunit beta type-4 OS=Mus musculus OX=10090 GN=Psmb4 PE=1 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 3.8e-54
Identity = 98/216 (45.37%), Postives = 153/216 (70.83%), Query Frame = 0


Query: 17  EAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSVERIKAIGKHSLLGAS 76
           E PI RTQ P VTGTSV+ +K+  G+++ +D+ GSYGS  R++++ RI  +   ++LGAS
Sbjct: 40  EGPITRTQNPMVTGTSVLGVKFDGGVVIAADMLGSYGSLARFRNISRIMRVNDSTMLGAS 99

Query: 77  GEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYNRRNKFNPLWNSLVLGG 136
           G+ +DFQ L + L ++++ + +  DG++  P+ IHS+LTR MY+RR+K NPLWN++V+GG
Sbjct: 100 GDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGG 159

Query: 137 VKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRD--EWHENLTFEEGVKLLEKCMR 196
             +G+ +LG V+M+GV +E   + TG+G +LA+P+LR+  E    L+  E  +L+E+CMR
Sbjct: 160 YADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARELVERCMR 219

Query: 197 VLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWN 231
           VL YRD  + N+ QIA +TE+GV I  P S +T W+
Sbjct: 220 VLYYRDARSYNRFQIATVTEKGVEIEGPLSAQTNWD 255

[Close]

BLAST of Carg03251 vs. ExPASy Swiss-Prot
Match: Q556Q0 (Proteasome subunit beta type-4 OS=Dictyostelium discoideum OX=44689 GN=psmB4-1 PE=3 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 5.0e-54
Identity = 102/213 (47.89%), Postives = 149/213 (69.95%), Query Frame = 0


Query: 21  QRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSVERIKAIGKHSLLGASGEIS 80
           Q+T  P VTGTSV+AIKY  G++M SD+  SYGS  R+ S ER+K  G ++++GASGE S
Sbjct: 35  QKTLDPIVTGTSVIAIKYDKGVVMGSDMLLSYGSLARFNSTERMKKFGGYTIVGASGEYS 94

Query: 81  DFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYNRRNKFNPLWNSLVLGGVKNG 140
           DFQ +   L++L+  D+  DDG+ L  +EI +YL RV+YN+RN+ NPLWN+LV+ G + G
Sbjct: 95  DFQSITNTLNDLVTDDHCMDDGSKLSSEEIWNYLARVLYNQRNRGNPLWNTLVVMGYQGG 154

Query: 141 QKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHE--NLTFEEGVKLLEKCMRVLLY 200
           + +LG V+++G  ++DD +TTG+G+H+A P+LR    E  N+  E+  +L++ C+RVL Y
Sbjct: 155 KSFLGKVDLVGTCYKDDIITTGYGSHIALPLLRKARDENPNMNLEQAKQLIQDCLRVLFY 214

Query: 201 RDRSAVNKLQIAKITEEGVTISQPYSLKTF-WN 231
           RD  +  K+QI+   EEG+ IS P  L TF WN
Sbjct: 215 RDARSSKKIQISVAGEEGIEISGPIELDTFHWN 247

[Close]

BLAST of Carg03251 vs. ExPASy Swiss-Prot
Match: P28070 (Proteasome subunit beta type-4 OS=Homo sapiens OX=9606 GN=PSMB4 PE=1 SV=4)

HSP 1 Score: 211.5 bits (537), Expect = 1.1e-53
Identity = 97/214 (45.33%), Postives = 152/214 (71.03%), Query Frame = 0


Query: 19  PIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSVERIKAIGKHSLLGASGE 78
           PI RTQ P VTGTSV+ +K++ G+++ +D+ GSYGS  R++++ RI  +   ++LGASG+
Sbjct: 42  PITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGD 101

Query: 79  ISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYNRRNKFNPLWNSLVLGGVK 138
            +DFQ L + L ++++ + +  DG++  P+ IHS+LTR MY+RR+K NPLWN++V+GG  
Sbjct: 102 YADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYA 161

Query: 139 NGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRD--EWHENLTFEEGVKLLEKCMRVL 198
           +G+ +LG V+M+GV +E   + TG+G +LA+P+LR+  E    L+  E   L+E+CMRVL
Sbjct: 162 DGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVL 221

Query: 199 LYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWN 231
            YRD  + N+ QIA +TE+GV I  P S +T W+
Sbjct: 222 YYRDARSYNRFQIATVTEKGVEIEGPLSTETNWD 255

[Close]

BLAST of Carg03251 vs. ExPASy TrEMBL
Match: A0A6J1J6N8 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111483953 PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.0e-139
Identity = 244/245 (99.59%), Postives = 244/245 (99.59%), Query Frame = 0


Query: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60
           MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS
Sbjct: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60

Query: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120
           VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN
Sbjct: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120

Query: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180
           RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL
Sbjct: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180

Query: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240
           TF EGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG
Sbjct: 181 TFVEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240

Query: 241 AEGSW 246
           AEGSW
Sbjct: 241 AEGSW 245

[Close]

BLAST of Carg03251 vs. ExPASy TrEMBL
Match: A0A6J1G834 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111451719 PE=3 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.0e-139
Identity = 242/245 (98.78%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60
           MSPVDSNQANHG+LCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS
Sbjct: 1   MSPVDSNQANHGILCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60

Query: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120
           VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN
Sbjct: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120

Query: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180
           RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL
Sbjct: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180

Query: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240
           T+EEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNY+AFENPTSG
Sbjct: 181 TYEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYAAFENPTSG 240

Query: 241 AEGSW 246
           AEGSW
Sbjct: 241 AEGSW 245

[Close]

BLAST of Carg03251 vs. ExPASy TrEMBL
Match: A0A6J1FW56 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111449042 PE=3 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.0e-139
Identity = 244/245 (99.59%), Postives = 244/245 (99.59%), Query Frame = 0


Query: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60
           MS VDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS
Sbjct: 1   MSSVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60

Query: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120
           VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN
Sbjct: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120

Query: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180
           RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL
Sbjct: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180

Query: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240
           TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG
Sbjct: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240

Query: 241 AEGSW 246
           AEGSW
Sbjct: 241 AEGSW 245

[Close]

BLAST of Carg03251 vs. ExPASy TrEMBL
Match: A0A5D3CQV9 (Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00450 PE=3 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 1.5e-138
Identity = 241/245 (98.37%), Postives = 244/245 (99.59%), Query Frame = 0


Query: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60
           MSP DSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILM++DLGGSYGSTLRYKS
Sbjct: 1   MSPSDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMIADLGGSYGSTLRYKS 60

Query: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120
           VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTR+MYN
Sbjct: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRLMYN 120

Query: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180
           RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL
Sbjct: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180

Query: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240
           TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG
Sbjct: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240

Query: 241 AEGSW 246
           AEGSW
Sbjct: 241 AEGSW 245

[Close]

BLAST of Carg03251 vs. ExPASy TrEMBL
Match: A0A1S3CLV2 (Proteasome subunit beta OS=Cucumis melo OX=3656 GN=LOC103501933 PE=3 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 1.5e-138
Identity = 241/245 (98.37%), Postives = 244/245 (99.59%), Query Frame = 0


Query: 1   MSPVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKS 60
           MSP DSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILM++DLGGSYGSTLRYKS
Sbjct: 1   MSPSDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMIADLGGSYGSTLRYKS 60

Query: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYN 120
           VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTR+MYN
Sbjct: 61  VERIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRLMYN 120

Query: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180
           RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL
Sbjct: 121 RRNKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENL 180

Query: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240
           TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG
Sbjct: 181 TFEEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSG 240

Query: 241 AEGSW 246
           AEGSW
Sbjct: 241 AEGSW 245

[Close]

BLAST of Carg03251 vs. TAIR 10
Match: AT1G56450.1 (20S proteasome beta subunit G1 )

HSP 1 Score: 415.2 bits (1066), Expect = 3.6e-116
Identity = 194/243 (79.84%), Postives = 220/243 (90.53%), Query Frame = 0


Query: 3   PVDSNQANHGLLCPEAPIQRTQYPYVTGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSVE 62
           P+D+  +   +   E   QRT YPYVTGTS+VAIKYKDG+LM SD+GGSYGSTLRYK++E
Sbjct: 7   PIDNGDS---MKIAEEESQRTLYPYVTGTSIVAIKYKDGVLMASDMGGSYGSTLRYKNIE 66

Query: 63  RIKAIGKHSLLGASGEISDFQELLRNLDELILYDNMWDDGNALGPKEIHSYLTRVMYNRR 122
           R+KAIGKHSLLGASGEISDFQE+LR LDEL L DNMWDDGN+LGPKEIH+YLTRVMYNRR
Sbjct: 67  RVKAIGKHSLLGASGEISDFQEILRYLDELTLNDNMWDDGNSLGPKEIHNYLTRVMYNRR 126

Query: 123 NKFNPLWNSLVLGGVKNGQKYLGTVNMIGVTFEDDHVTTGFGNHLARPILRDEWHENLTF 182
           NKFNPLWN+LVLGGVKNG+ YLG V+MIGV+FEDDHV TGFGNHLARPILRDEWH +L+F
Sbjct: 127 NKFNPLWNTLVLGGVKNGKSYLGMVSMIGVSFEDDHVATGFGNHLARPILRDEWHADLSF 186

Query: 183 EEGVKLLEKCMRVLLYRDRSAVNKLQIAKITEEGVTISQPYSLKTFWNYSAFENPTSGAE 242
           E+GVKLLEKCMRVLLYRDRSA+NKLQIAKITEEGVT+SQPYSLKT+W +S+F NPT+GA 
Sbjct: 187 EDGVKLLEKCMRVLLYRDRSAINKLQIAKITEEGVTVSQPYSLKTYWEFSSFHNPTAGAA 246

Query: 243 GSW 246
           GSW
Sbjct: 247 GSW 246

[Close]

BLAST of Carg03251 vs. TAIR 10
Match: AT5G40580.1 (20S proteasome beta subunit PBB2 )

HSP 1 Score: 56.6 bits (135), Expect = 3.2e-08
Identity = 43/186 (23.12%), Postives = 89/186 (47.85%), Query Frame = 0


Query: 29  TGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSVERIKAIGKHSLLGASGEISDFQELLRN 88
           TGT++V + +KDG+++ +D   + G  +  K+ E+I  +  +     +G  +D  E + +
Sbjct: 38  TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAAD-TEAVTD 97

Query: 89  LDELILYDNMWDDGNALGPKEIHSYLTRVMYNRRNKFNPLWNSLVLGGVKNGQKYLGTVN 148
           +    L  + +  G         + L + +++ +   +    +LVLGGV     +L T+ 
Sbjct: 98  MVSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSA---ALVLGGVDITGPHLHTIY 157

Query: 149 MIGVTFEDDHVTTGFGNHLARPILRDEWHENLTFEEGVKLLEKCMRVLLYRDRSAVNKLQ 208
             G T      T G G+  A  +   ++ E LT +EG+KL+ + +   ++ D  + + + 
Sbjct: 158 PHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAEAICSGIFNDLGSGSNVD 217

Query: 209 IAKITE 215
           I  IT+
Sbjct: 218 ICVITK 219

[Close]

BLAST of Carg03251 vs. TAIR 10
Match: AT5G40580.2 (20S proteasome beta subunit PBB2 )

HSP 1 Score: 56.6 bits (135), Expect = 3.2e-08
Identity = 43/186 (23.12%), Postives = 89/186 (47.85%), Query Frame = 0


Query: 29  TGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSVERIKAIGKHSLLGASGEISDFQELLRN 88
           TGT++V + +KDG+++ +D   + G  +  K+ E+I  +  +     +G  +D  E + +
Sbjct: 38  TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAAD-TEAVTD 97

Query: 89  LDELILYDNMWDDGNALGPKEIHSYLTRVMYNRRNKFNPLWNSLVLGGVKNGQKYLGTVN 148
           +    L  + +  G         + L + +++ +   +    +LVLGGV     +L T+ 
Sbjct: 98  MVSSQLRLHRYQTGRDSRVVTALTLLKKHLFSYQGHVSA---ALVLGGVDITGPHLHTIY 157

Query: 149 MIGVTFEDDHVTTGFGNHLARPILRDEWHENLTFEEGVKLLEKCMRVLLYRDRSAVNKLQ 208
             G T      T G G+  A  +   ++ E LT +EG+KL+ + +   ++ D  + + + 
Sbjct: 158 PHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAEAICSGIFNDLGSGSNVD 217

Query: 209 IAKITE 215
           I  IT+
Sbjct: 218 ICVITK 219

[Close]

BLAST of Carg03251 vs. TAIR 10
Match: AT3G27430.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 7.2e-08
Identity = 44/186 (23.66%), Postives = 89/186 (47.85%), Query Frame = 0


Query: 29  TGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSVERIKAIGKHSLLGASGEISDFQELLRN 88
           TGT++V + +KDG+++ +D   + G  +  K+ E+I  +  +     +G  +D  E + +
Sbjct: 38  TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAAD-TEAVTD 97

Query: 89  LDELILYDNMWDDGNALGPKEIHSYLTRVMYNRRNKFNPLWNSLVLGGVKNGQKYLGTVN 148
           +    L  + +  G       + + LT +  +  +    +  +LVLGGV     +L T+ 
Sbjct: 98  MVSSQLRLHRYQTGR---DSRVITALTLLKKHLFSYQGHVSAALVLGGVDITGPHLHTIY 157

Query: 149 MIGVTFEDDHVTTGFGNHLARPILRDEWHENLTFEEGVKLLEKCMRVLLYRDRSAVNKLQ 208
             G T      T G G+  A  +   ++ E LT +EG+KL+ + +   ++ D  + + + 
Sbjct: 158 PHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAESICSGIFNDLGSGSNVD 217

Query: 209 IAKITE 215
           I  IT+
Sbjct: 218 ICVITK 219

[Close]

BLAST of Carg03251 vs. TAIR 10
Match: AT3G27430.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 55.5 bits (132), Expect = 7.2e-08
Identity = 44/186 (23.66%), Postives = 89/186 (47.85%), Query Frame = 0


Query: 29  TGTSVVAIKYKDGILMVSDLGGSYGSTLRYKSVERIKAIGKHSLLGASGEISDFQELLRN 88
           TGT++V + +KDG+++ +D   + G  +  K+ E+I  +  +     +G  +D  E + +
Sbjct: 38  TGTTIVGLIFKDGVILGADTRATEGPIVADKNCEKIHYMAPNIYCCGAGTAAD-TEAVTD 97

Query: 89  LDELILYDNMWDDGNALGPKEIHSYLTRVMYNRRNKFNPLWNSLVLGGVKNGQKYLGTVN 148
           +    L  + +  G       + + LT +  +  +    +  +LVLGGV     +L T+ 
Sbjct: 98  MVSSQLRLHRYQTGR---DSRVITALTLLKKHLFSYQGHVSAALVLGGVDITGPHLHTIY 157

Query: 149 MIGVTFEDDHVTTGFGNHLARPILRDEWHENLTFEEGVKLLEKCMRVLLYRDRSAVNKLQ 208
             G T      T G G+  A  +   ++ E LT +EG+KL+ + +   ++ D  + + + 
Sbjct: 158 PHGSTDTLPFATMGSGSLAAMSVFEAKYKEGLTRDEGIKLVAESICSGIFNDLGSGSNVD 217

Query: 209 IAKITE 215
           I  IT+
Sbjct: 218 ICVITK 219

The following BLAST results are available for this feature:

BLAST of Carg03251 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010616.1	9.7e-140	100.00	Proteasome subunit beta type-4 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023512002.1	2.2e-139	99.59	proteasome subunit beta type-4-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022986112.1	6.3e-139	99.59	proteasome subunit beta type-4-like isoform X1 [Cucurbita maxima]	[more]
XP_022948016.1	8.2e-139	98.78	proteasome subunit beta type-4-like isoform X1 [Cucurbita moschata] >XP_02353228...	[more]
XP_022944642.1	8.2e-139	99.59	proteasome subunit beta type-4-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Carg03251 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q7DLR9	5.0e-115	79.84	Proteasome subunit beta type-4 OS=Arabidopsis thaliana OX=3702 GN=PBG1 PE=1 SV=2	[more]
Q3T108	1.3e-54	44.59	Proteasome subunit beta type-4 OS=Bos taurus OX=9913 GN=PSMB4 PE=1 SV=1	[more]
P99026	3.8e-54	45.37	Proteasome subunit beta type-4 OS=Mus musculus OX=10090 GN=Psmb4 PE=1 SV=1	[more]
Q556Q0	5.0e-54	47.89	Proteasome subunit beta type-4 OS=Dictyostelium discoideum OX=44689 GN=psmB4-1 P...	[more]
P28070	1.1e-53	45.33	Proteasome subunit beta type-4 OS=Homo sapiens OX=9606 GN=PSMB4 PE=1 SV=4	[more]

BLAST of Carg03251 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J6N8	3.0e-139	99.59	Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111483953 PE=3 SV=1	[more]
A0A6J1G834	4.0e-139	98.78	Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111451719 PE=3 SV=1	[more]
A0A6J1FW56	4.0e-139	99.59	Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111449042 PE=3 SV=1	[more]
A0A5D3CQV9	1.5e-138	98.37	Proteasome subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CLV2	1.5e-138	98.37	Proteasome subunit beta OS=Cucumis melo OX=3656 GN=LOC103501933 PE=3 SV=1	[more]

BLAST of Carg03251 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56450.1	3.6e-116	79.84	20S proteasome beta subunit G1	[more]
AT5G40580.1	3.2e-08	23.12	20S proteasome beta subunit PBB2	[more]
AT5G40580.2	3.2e-08	23.12	20S proteasome beta subunit PBB2	[more]
AT3G27430.1	7.2e-08	23.66	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT3G27430.2	7.2e-08	23.66	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016295	Proteasome subunit beta 4	PIRSF	PIRSF001213	MCP	coord: 2..243 e-value: 1.2E-94 score: 314.1
IPR016295	Proteasome subunit beta 4	CDD	cd03760	proteasome_beta_type_4	coord: 29..222 e-value: 1.5893E-114 score: 324.524
IPR029055	Nucleophile aminohydrolases, N-terminal	GENE3D	3.60.20.10	Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1	coord: 27..241 e-value: 3.6E-60 score: 205.1
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 24..232
IPR001353	Proteasome, subunit alpha/beta	PFAM	PF00227	Proteasome	coord: 29..212 e-value: 3.0E-31 score: 108.4
None	No IPR available	PANTHER	PTHR11599	PROTEASOME SUBUNIT ALPHA/BETA	coord: 12..228
None	No IPR available	PANTHER	PTHR11599:SF177	PROTEASOME SUBUNIT BETA	coord: 12..228
IPR023333	Proteasome B-type subunit	PROSITE	PS51476	PROTEASOME_BETA_2	coord: 30..212 score: 37.955875

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg03251-RA	Carg03251-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010498	proteasomal protein catabolic process
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0019774	proteasome core complex, beta-subunit complex
cellular_component	GO:0005839	proteasome core complex
molecular_function	GO:0004298	threonine-type endopeptidase activity