Carg02689 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg02689
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRING-type domain-containing protein
LocationCarg_Chr02: 7394420 .. 7397382 (+)
RNA-Seq ExpressionCarg02689
SyntenyCarg02689
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACTTCTGATTTTATTTGCAGAGAACTCTTCTGGATCGCCATTAGAGAATATTCTCGAGCATCTTTTTTTTCTTCTCTTTTGCTTCAGAATAATTCTTCAGCATCTCCTTTCATTTCAAGTTTCGATTTCTGCTGAATAACTGTTGTTCTGGGTTCTGTGTGATCTAATTTATAAAGCGAGAAGAATCGGAGGTGAAACCCTCGTGTTCTCGCCATGGCTTCCTCTGGAATCTCTACTGAAAGCGCTGAATTGTCTGATGCCGTTCAAATACTGGAAGTTTGCAACCAGGTTTTAATCTAGTTTTTCACTTTTGAAGTTGTTACACTTTCTTGCGTAAAACTTGGCGCGAGAAATGGTTATTTTTTGTGTTGAGAGTTCGAATTGGAAGTATCGTTGATCTAATCTTGTTTTCTAGATTTTCGCTGGTGAAGGACTAATTGAAGTGTCATCGTCGGTTTAGTCTTGTTTTATTTCATTTTCAAGATTTTCGGGGCGTCGATAAGCGTATTTCTGCTTTTTAATATCAAGGAAGTACGTGGTCTAAATGAATGGATTTGATGAGCGTGTTTTTTTTCTTTTACTCTGAAGATCCGTAGGAAGCGGGAGCTATTGTTAACATTCATTGATTTCCGTTATCCGATTGCCTGCTAATAGATCACCGTTGTTTTCAAATTGTGAATGGTCACCTGCGCATGCTATTTTATTTTCCAATGAAAGTTCTTAATTCTTGCCCTTGGTGTGGCATATTATTGAAGTGTCTCTTTTCAAACATTAGTCATTTGCATAAATATATTTTGAGGATTGCTAAGTTGCTGAACTTCTCTGTTCAGATCGAAATGGGAAAGAAGGTAGAGATTCAAGGAACAGGTCATGGGAGTCATGATGGGGTTTGTGCAATATGCTTAAATAAGATAGCTCTCCAAGAAACTGCCTTAGTGAGGGGTTGCGAGCATGCCTATTGGTTTGTTCTTATTGATTAATCTTTCATCTATCTATTAATAGTTTCTATAGCACGACTTTCATATGCTATACATTAGGTAATTTGTTGATCCGTTCCTTTTCTGTATTTTGTATGGGAATCTTTTTTGCAGTCAAATGTTGACGTTCTATAGATGGTGCAATTCTTATGCTGCATTTATCATATATATTTCATTTCATTGTGGTTCTATGCTTGAATTGGAGTTGGAGTCTTAAGATCTTGCGAACATATTCCTGGGACTCCGCTAAACTTTATAGGAGAAAATTCATTGTATCCATATTGCTATTGGTACAATGTTTCTAATTGTGCTTCAGGTTGCTGGGATTTAATGGAAGAGGTATGCTATTTTAGATTTATAGCCCAAGTATCTTGGAGTCGGAGGAAATGTATGGTTCAGAGTCTGATGGTTAATCTGTTAATTCTCGTCTTATTTAGTATGTTAATAATTAATTATTGATTATTGAAGTTAGAATGCTTGCTTGGTGAGACTGGGATAGAAACCTGTTGTACTCGGTACTCAGTGACTTATTGTGAGCCTATGAATCTCATAGTTGTCGGCTTTGGTAACGTGTTCCTATTCTACGTAGGGTTGGATACTTGTTAAATCGGACAAATATCAAACACTGTGTTGAACATTTATGGATTTCAATAGACCTGAATCAGACGCATATTGAACAATTATTAGGCATTTGAACTAAGTGTTGAAACATATGTTGAACAAGGCTGGGATGTTTAGAAAATGTCAATGTCCGTGCTAGTGTCTATGCTTCTTTGTCATTAGCCGATTACTCTTTCATCACCTCCAAATTGTCATCACATACTCGATGCTTTTGGTGTCTGTTGCAAAGTTATGTTTTATTACTTGGTGTGGGGAACATTTCCCCTATGACTATTGCATTTAGTTTTCACTTTCAGCTTAGGAACTTATCAGTAATAATTAAACCAAACAAAAAATGAAGTGGCAAATGGTTAAGTGAATTGTCTATTGTTTTCCAACCACTGAAGCATTACAAATATTTTCCTTGCTTTTTTTGAGGAATAGTGTGATCTTTTACGGTTTATACACATGGGCATTATATTCTTTCATTAATGCATTGTTTTACTTTCATGAAGTGCGACTTGCATCCTTCGATGGGCCTCATATAATAAGAATCCGACATGTCCACAATGTAAACATCCATTTGAGTTCCTGATTGTGCATCGTTCTCTTGATGGGAGGTATGCCCCTCCATCATTCTATGGACCTTTTATAGTTTAGAGTCGCAGTATCATTTTAAATTAATGTTCTTCTCTATCCATTTATTGTCTGCGTATTGTAGCGTCCATGACTTTATGTTTGAGGAGAGCGTATGCCTACTTCTGAGAGCTTCATGGTATATACCTCTGATCGTTGAAGATCGGGAAGAAACCTATGAAGATCCTGATGAGTACTACCCTTACGAGGATGATGACGAAGAGTTTGAGGAAGCTTACCTGGGTGCATCGTCGAGTCTCCGCATCGGCAATCGAAGGTGGGGAGACAATGGATATGTGAGGGGAGGTCGACAAGAAGCACGACCAGTTATTAGGTCAACTGTTCCAGTGCATGCAGGAGCTAGTTCATCACGTGAGCCTACAAAAAAAGATGCTTCCAAAGATAAAACCGGTCGACGAGCTAAAAGGGCCTTGAAACGGGAAGCTGCTGATAAAGCTGCCGCAGCTAAACATCAGGAACATCTAGCTAGGTTTGGGCGGAAGTGATTCAAGTTCCTGTATATAACAAGCCTTGCGCTTAGACCCTGTGTACAGTTCTAAGATTCAATTTACTTTCACAGTTGGAAGTACCTTACCTCCCCTGAGCCTGGTGGCTCCCCAATTAAATCTATGGTGGGGATTATACTTGCATGGTATAAAGGACACCCCAATGAGTTCTTTGTATGAACATTGAATCTCAAAATCAATCATAGAACAATTGTTATGGTCAGATTTTCTGTTCTTTTTTAG

mRNA sequence

AACTTCTGATTTTATTTGCAGAGAACTCTTCTGGATCGCCATTAGAGAATATTCTCGAGCATCTTTTTTTTCTTCTCTTTTGCTTCAGAATAATTCTTCAGCATCTCCTTTCATTTCAAGTTTCGATTTCTGCTGAATAACTGTTGTTCTGGGTTCTGTGTGATCTAATTTATAAAGCGAGAAGAATCGGAGGTGAAACCCTCGTGTTCTCGCCATGGCTTCCTCTGGAATCTCTACTGAAAGCGCTGAATTGTCTGATGCCGTTCAAATACTGGAAGTTTGCAACCAGATCGAAATGGGAAAGAAGGTAGAGATTCAAGGAACAGGTCATGGGAGTCATGATGGGGTTTGTGCAATATGCTTAAATAAGATAGCTCTCCAAGAAACTGCCTTAGTGAGGGGTTGCGAGCATGCCTATTGTGCGACTTGCATCCTTCGATGGGCCTCATATAATAAGAATCCGACATGTCCACAATGTAAACATCCATTTGAGTTCCTGATTGTGCATCGTTCTCTTGATGGGAGCGTCCATGACTTTATGTTTGAGGAGAGCGTATGCCTACTTCTGAGAGCTTCATGGTATATACCTCTGATCGTTGAAGATCGGGAAGAAACCTATGAAGATCCTGATGAGTACTACCCTTACGAGGATGATGACGAAGAGTTTGAGGAAGCTTACCTGGGTGCATCGTCGAGTCTCCGCATCGGCAATCGAAGGTGGGGAGACAATGGATATGTGAGGGGAGGTCGACAAGAAGCACGACCAGTTATTAGGTCAACTGTTCCAGTGCATGCAGGAGCTAGTTCATCACGTGAGCCTACAAAAAAAGATGCTTCCAAAGATAAAACCGGTCGACGAGCTAAAAGGGCCTTGAAACGGGAAGCTGCTGATAAAGCTGCCGCAGCTAAACATCAGGAACATCTAGCTAGGTTTGGGCGGAAGTGATTCAAGTTCCTGTATATAACAAGCCTTGCGCTTAGACCCTGTGTACAGTTCTAAGATTCAATTTACTTTCACAGTTGGAAGTACCTTACCTCCCCTGAGCCTGGTGGCTCCCCAATTAAATCTATGGTGGGGATTATACTTGCATGGTATAAAGGACACCCCAATGAGTTCTTTGTATGAACATTGAATCTCAAAATCAATCATAGAACAATTGTTATGGTCAGATTTTCTGTTCTTTTTTAG

Coding sequence (CDS)

ATGGCTTCCTCTGGAATCTCTACTGAAAGCGCTGAATTGTCTGATGCCGTTCAAATACTGGAAGTTTGCAACCAGATCGAAATGGGAAAGAAGGTAGAGATTCAAGGAACAGGTCATGGGAGTCATGATGGGGTTTGTGCAATATGCTTAAATAAGATAGCTCTCCAAGAAACTGCCTTAGTGAGGGGTTGCGAGCATGCCTATTGTGCGACTTGCATCCTTCGATGGGCCTCATATAATAAGAATCCGACATGTCCACAATGTAAACATCCATTTGAGTTCCTGATTGTGCATCGTTCTCTTGATGGGAGCGTCCATGACTTTATGTTTGAGGAGAGCGTATGCCTACTTCTGAGAGCTTCATGGTATATACCTCTGATCGTTGAAGATCGGGAAGAAACCTATGAAGATCCTGATGAGTACTACCCTTACGAGGATGATGACGAAGAGTTTGAGGAAGCTTACCTGGGTGCATCGTCGAGTCTCCGCATCGGCAATCGAAGGTGGGGAGACAATGGATATGTGAGGGGAGGTCGACAAGAAGCACGACCAGTTATTAGGTCAACTGTTCCAGTGCATGCAGGAGCTAGTTCATCACGTGAGCCTACAAAAAAAGATGCTTCCAAAGATAAAACCGGTCGACGAGCTAAAAGGGCCTTGAAACGGGAAGCTGCTGATAAAGCTGCCGCAGCTAAACATCAGGAACATCTAGCTAGGTTTGGGCGGAAGTGA

Protein sequence

MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETALVRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRASWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQEARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARFGRK
Homology
BLAST of Carg02689 vs. NCBI nr
Match: KAG6605938.1 (hypothetical protein SDJN03_03255, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035883.1 hypothetical protein SDJN02_02682 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 486.9 bits (1252), Expect = 1.0e-133
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60
           MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180
           SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ
Sbjct: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240
           EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF
Sbjct: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

BLAST of Carg02689 vs. NCBI nr
Match: XP_022958365.1 (uncharacterized protein LOC111459609 [Cucurbita moschata])

HSP 1 Score: 483.8 bits (1244), Expect = 8.7e-133
Identity = 242/243 (99.59%), Postives = 242/243 (99.59%), Query Frame = 0

Query: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60
           MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180
           SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ
Sbjct: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240
           EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQE LARF
Sbjct: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQERLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

BLAST of Carg02689 vs. NCBI nr
Match: XP_023533579.1 (uncharacterized protein LOC111795401 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 482.3 bits (1240), Expect = 2.5e-132
Identity = 241/243 (99.18%), Postives = 241/243 (99.18%), Query Frame = 0

Query: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60
           MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180
           SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ
Sbjct: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240
           EARPVIRSTVPVHAGASSSREP KKD SKDKTGRRAKRALKREAADKAAAAKHQEHLARF
Sbjct: 181 EARPVIRSTVPVHAGASSSREPIKKDDSKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

BLAST of Carg02689 vs. NCBI nr
Match: XP_022995136.1 (uncharacterized protein LOC111490767 [Cucurbita maxima])

HSP 1 Score: 482.3 bits (1240), Expect = 2.5e-132
Identity = 240/243 (98.77%), Postives = 241/243 (99.18%), Query Frame = 0

Query: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60
           MASSGISTESAELSDAVQILEVCNQIEMGKKVEI GTGHGSHDGVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIHGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180
           SWY+PLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ
Sbjct: 121 SWYVPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240
           EARPVIRSTVPVHAGASSSREP KKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF
Sbjct: 181 EARPVIRSTVPVHAGASSSREPIKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

BLAST of Carg02689 vs. NCBI nr
Match: XP_022153003.1 (uncharacterized protein LOC111020607 [Momordica charantia])

HSP 1 Score: 432.6 bits (1111), Expect = 2.3e-117
Identity = 210/242 (86.78%), Postives = 224/242 (92.56%), Query Frame = 0

Query: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60
           M SSGIS E AE+SDAVQ LE+C+Q+++GKKVEIQGTGHGSHDG CAICLNKIALQETAL
Sbjct: 1   MTSSGISIEGAEMSDAVQELEICDQVKIGKKVEIQGTGHGSHDGACAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120
           VRGCEHAYC TCILRWASY + PTCPQCKHPFEFLIVHRSLDG +HD+MFEESVCLLLRA
Sbjct: 61  VRGCEHAYCVTCILRWASYTQKPTCPQCKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRA 120

Query: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180
           SWYIPL+VEDREETY+DP+EYYPYEDDDEE EE YLGASSSLRIGNRRWGDNGYVR GRQ
Sbjct: 121 SWYIPLVVEDREETYDDPEEYYPYEDDDEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQ 180

Query: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240
           EARP+ R +VP  A ASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLAR 
Sbjct: 181 EARPIFRPSVPEFAAASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARL 240

Query: 241 GR 243
           GR
Sbjct: 241 GR 242

BLAST of Carg02689 vs. ExPASy Swiss-Prot
Match: Q99590 (Protein SCAF11 OS=Homo sapiens OX=9606 GN=SCAF11 PE=1 SV=2)

HSP 1 Score: 51.2 bits (121), Expect = 1.9e-05
Identity = 22/60 (36.67%), Postives = 32/60 (53.33%), Query Frame = 0

Query: 46  CAICLNKIALQETALVRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSV 105
           C ICLN +  +E      C H +C TCIL+WA      +CP  + PF+ +    +L+G V
Sbjct: 41  CPICLNCLLEKEVGFPESCNHVFCMTCILKWA--ETLASCPIDRKPFQAVFKFSALEGYV 98

BLAST of Carg02689 vs. ExPASy TrEMBL
Match: A0A6J1H396 (uncharacterized protein LOC111459609 OS=Cucurbita moschata OX=3662 GN=LOC111459609 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 4.2e-133
Identity = 242/243 (99.59%), Postives = 242/243 (99.59%), Query Frame = 0

Query: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60
           MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180
           SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ
Sbjct: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240
           EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQE LARF
Sbjct: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQERLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

BLAST of Carg02689 vs. ExPASy TrEMBL
Match: A0A6J1K158 (uncharacterized protein LOC111490767 OS=Cucurbita maxima OX=3661 GN=LOC111490767 PE=4 SV=1)

HSP 1 Score: 482.3 bits (1240), Expect = 1.2e-132
Identity = 240/243 (98.77%), Postives = 241/243 (99.18%), Query Frame = 0

Query: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60
           MASSGISTESAELSDAVQILEVCNQIEMGKKVEI GTGHGSHDGVCAICLNKIALQETAL
Sbjct: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIHGTGHGSHDGVCAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120
           VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA
Sbjct: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120

Query: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180
           SWY+PLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ
Sbjct: 121 SWYVPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180

Query: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240
           EARPVIRSTVPVHAGASSSREP KKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF
Sbjct: 181 EARPVIRSTVPVHAGASSSREPIKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240

Query: 241 GRK 244
           GRK
Sbjct: 241 GRK 243

BLAST of Carg02689 vs. ExPASy TrEMBL
Match: A0A6J1DGD3 (uncharacterized protein LOC111020607 OS=Momordica charantia OX=3673 GN=LOC111020607 PE=4 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 1.1e-117
Identity = 210/242 (86.78%), Postives = 224/242 (92.56%), Query Frame = 0

Query: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60
           M SSGIS E AE+SDAVQ LE+C+Q+++GKKVEIQGTGHGSHDG CAICLNKIALQETAL
Sbjct: 1   MTSSGISIEGAEMSDAVQELEICDQVKIGKKVEIQGTGHGSHDGACAICLNKIALQETAL 60

Query: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120
           VRGCEHAYC TCILRWASY + PTCPQCKHPFEFLIVHRSLDG +HD+MFEESVCLLLRA
Sbjct: 61  VRGCEHAYCVTCILRWASYTQKPTCPQCKHPFEFLIVHRSLDGGIHDYMFEESVCLLLRA 120

Query: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180
           SWYIPL+VEDREETY+DP+EYYPYEDDDEE EE YLGASSSLRIGNRRWGDNGYVR GRQ
Sbjct: 121 SWYIPLVVEDREETYDDPEEYYPYEDDDEELEEDYLGASSSLRIGNRRWGDNGYVRAGRQ 180

Query: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240
           EARP+ R +VP  A ASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLAR 
Sbjct: 181 EARPIFRPSVPEFAAASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARL 240

Query: 241 GR 243
           GR
Sbjct: 241 GR 242

BLAST of Carg02689 vs. ExPASy TrEMBL
Match: A0A6J1FXD4 (uncharacterized protein LOC111448431 OS=Cucurbita moschata OX=3662 GN=LOC111448431 PE=4 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 4.8e-113
Identity = 207/241 (85.89%), Postives = 221/241 (91.70%), Query Frame = 0

Query: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60
           M SSGIS +SAELSDAVQ L+VC+Q+EM KK+EIQGTGHGSHDG CAICLNKI+L ETAL
Sbjct: 1   MTSSGISLKSAELSDAVQDLKVCDQVEMWKKLEIQGTGHGSHDGACAICLNKISLPETAL 60

Query: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120
           VRGCEHAYC TCILRWASY + PTCPQCKHPFEFLIVHRSLDGS+HD+MFEESVCLLLRA
Sbjct: 61  VRGCEHAYCTTCILRWASYTQKPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120

Query: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180
           SWY PLIV+DREETY+DP+EYYPYED DEE EEAYLGAS SLRIGNRRWGDNGYVR GRQ
Sbjct: 121 SWYEPLIVKDREETYDDPEEYYPYEDQDEELEEAYLGASPSLRIGNRRWGDNGYVRAGRQ 180

Query: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240
           EARPV+R   P  +GASSSREPT KDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF
Sbjct: 181 EARPVLR---PEFSGASSSREPTTKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 238

Query: 241 G 242
           G
Sbjct: 241 G 238

BLAST of Carg02689 vs. ExPASy TrEMBL
Match: A0A6J1JFP2 (uncharacterized protein LOC111484597 OS=Cucurbita maxima OX=3661 GN=LOC111484597 PE=4 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 6.1e-108
Identity = 203/241 (84.23%), Postives = 217/241 (90.04%), Query Frame = 0

Query: 1   MASSGISTESAELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETAL 60
           M SSGIS +SAELSDAVQ L+VC+Q EM KK+E+Q TGHGSH G CAICLNKI+L ETAL
Sbjct: 1   MTSSGISLKSAELSDAVQDLKVCDQEEMWKKLEMQRTGHGSH-GSCAICLNKISLPETAL 60

Query: 61  VRGCEHAYCATCILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRA 120
           VRGCEHAYC TCILRWASY + PTCPQCKHPFEFLIVHRSLDGS+HD+MFEESVCLLLRA
Sbjct: 61  VRGCEHAYCTTCILRWASYTQKPTCPQCKHPFEFLIVHRSLDGSIHDYMFEESVCLLLRA 120

Query: 121 SWYIPLIVEDREETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQ 180
           SWY PLIV+DREETY+DP+EYYPYED DEE EEAYLG S SLRIGNRRWGDNGYVR GRQ
Sbjct: 121 SWYEPLIVKDREETYDDPEEYYPYEDQDEELEEAYLGDSPSLRIGNRRWGDNGYVRAGRQ 180

Query: 181 EARPVIRSTVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 240
           EARPV+R   P  +GASSSREPT KDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF
Sbjct: 181 EARPVLR---PEFSGASSSREPTTKDASKDKTGRRAKRALKREAADKAAAAKHQEHLARF 237

Query: 241 G 242
           G
Sbjct: 241 G 237

BLAST of Carg02689 vs. TAIR 10
Match: AT5G19430.1 (RING/U-box superfamily protein )

HSP 1 Score: 256.5 bits (654), Expect = 2.1e-68
Identity = 139/245 (56.73%), Postives = 176/245 (71.84%), Query Frame = 0

Query: 12  ELSDAVQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETALVRGCEHAYCAT 71
           ++ D +Q L + NQ +   + EI     G+H G CAICL++I LQETA+V+GCEH YC T
Sbjct: 12  QVIDGIQDLALQNQDKKKIQEEIHEPSFGNHGGCCAICLSEIPLQETAMVKGCEHTYCVT 71

Query: 72  CILRWASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRASWYIPLIVEDR 131
           CILRWAS  ++PTCPQCKHPF+FL VHR+LDGS+ DF+FEESVCLLLRASW++PL V ++
Sbjct: 72  CILRWASCKESPTCPQCKHPFDFLSVHRALDGSIEDFLFEESVCLLLRASWFVPLDVVEQ 131

Query: 132 EE----TYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQEARPVIR 191
                  ++D D  Y YED+D++ +E YL   SSLRIGNRRWG NG+VR GRQEARPV R
Sbjct: 132 ASYSHGYHDDFDIPYDYEDEDDDLDEFYL-HGSSLRIGNRRWGGNGFVRSGRQEARPVQR 191

Query: 192 -----STVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKA----AAAKHQEHLA 244
                S+    +G+SSS    K+  + + TGRRAKRA+KREAA+KA    AAAKH+ HL 
Sbjct: 192 YSGSGSSSSSSSGSSSSEPKDKQVKTTNTTGRRAKRAMKREAANKAAEVTAAAKHEAHLV 251

BLAST of Carg02689 vs. TAIR 10
Match: AT5G19430.2 (RING/U-box superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 4.0e-67
Identity = 138/239 (57.74%), Postives = 172/239 (71.97%), Query Frame = 0

Query: 20  LEVCNQIEMGKKV--EIQGTGHGSHDGVCAICLNKIALQETALVRGCEHAYCATCILRWA 79
           L + + I   KK+  EI     G+H G CAICL++I LQETA+V+GCEH YC TCILRWA
Sbjct: 3   LALLHNIRDKKKIQEEIHEPSFGNHGGCCAICLSEIPLQETAMVKGCEHTYCVTCILRWA 62

Query: 80  SYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRASWYIPLIVEDREE---- 139
           S  ++PTCPQCKHPF+FL VHR+LDGS+ DF+FEESVCLLLRASW++PL V ++      
Sbjct: 63  SCKESPTCPQCKHPFDFLSVHRALDGSIEDFLFEESVCLLLRASWFVPLDVVEQASYSHG 122

Query: 140 TYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQEARPVIR-----S 199
            ++D D  Y YED+D++ +E YL   SSLRIGNRRWG NG+VR GRQEARPV R     S
Sbjct: 123 YHDDFDIPYDYEDEDDDLDEFYL-HGSSLRIGNRRWGGNGFVRSGRQEARPVQRYSGSGS 182

Query: 200 TVPVHAGASSSREPTKKDASKDKTGRRAKRALKREAADKA----AAAKHQEHLARFGRK 244
           +    +G+SSS    K+  + + TGRRAKRA+KREAA+KA    AAAKH+ HL R GRK
Sbjct: 183 SSSSSSGSSSSEPKDKQVKTTNTTGRRAKRAMKREAANKAAEVTAAAKHEAHLVRLGRK 240

BLAST of Carg02689 vs. TAIR 10
Match: AT5G12310.1 (RING/U-box superfamily protein )

HSP 1 Score: 237.7 bits (605), Expect = 1.0e-62
Identity = 136/243 (55.97%), Postives = 164/243 (67.49%), Query Frame = 0

Query: 17  VQILEVCNQIEMGKKVEIQGTGHGSHDGVCAICLNKIALQETALVRGCEHAYCATCILRW 76
           V +L + +Q E            G+H G CAICL+ I LQETA+V+GCEHAYC TCILRW
Sbjct: 14  VDVLPLQDQDEKKTPQVFNELSFGNHGGCCAICLDTIPLQETAMVKGCEHAYCVTCILRW 73

Query: 77  ASYNKNPTCPQCKHPFEFLIVHRSLDGSVHDFMFEESVCLLLRASWYIPLIVEDR----- 136
           ASY + PTCPQCK PF+FL VHR+LDGSV DFMFEESVCLLLRASW+ PL   +R     
Sbjct: 74  ASYKEKPTCPQCKLPFDFLNVHRALDGSVEDFMFEESVCLLLRASWFQPLEAVERVSDND 133

Query: 137 --EETYEDPDEYYPYEDDDEEFEEAYLGASSSLRIGNRRWGDNGYVRGGRQEARPVIRST 196
                ++ P EY   ++DD++ +E Y+   SSLR+GNRRWGDNG+VR GRQEARPV    
Sbjct: 134 NFNYDFDIPPEYEEEDEDDDDLDEFYM-QGSSLRLGNRRWGDNGFVRAGRQEARPVQHKN 193

Query: 197 VPVHA-----GASSSREPTKKDASKDKTGRRAKRALKREAADKA----AAAKHQEHLARF 244
               A      +SSS EP +K +S   TGRRAKRA+KREAA+KA    AAAKH+  L R 
Sbjct: 194 RGGQASGSEPASSSSCEPKEKTSSA-ITGRRAKRAMKREAANKAAEAVAAAKHEALLVRL 253

BLAST of Carg02689 vs. TAIR 10
Match: AT3G30460.1 (RING/U-box superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.3e-04
Identity = 22/49 (44.90%), Postives = 26/49 (53.06%), Query Frame = 0

Query: 45  VCAICLNKIALQETALVRGCEHAYCATCILRWASYNKNPTCPQCKHPFE 94
           VCAIC  ++A  E      C H Y   CI  W S N+N TCP C+H  E
Sbjct: 96  VCAICREELAANERLSELPCRHYYHKECISNWLS-NRN-TCPLCRHNVE 142

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6605938.11.0e-133100.00hypothetical protein SDJN03_03255, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022958365.18.7e-13399.59uncharacterized protein LOC111459609 [Cucurbita moschata][more]
XP_023533579.12.5e-13299.18uncharacterized protein LOC111795401 [Cucurbita pepo subsp. pepo][more]
XP_022995136.12.5e-13298.77uncharacterized protein LOC111490767 [Cucurbita maxima][more]
XP_022153003.12.3e-11786.78uncharacterized protein LOC111020607 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q995901.9e-0536.67Protein SCAF11 OS=Homo sapiens OX=9606 GN=SCAF11 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1H3964.2e-13399.59uncharacterized protein LOC111459609 OS=Cucurbita moschata OX=3662 GN=LOC1114596... [more]
A0A6J1K1581.2e-13298.77uncharacterized protein LOC111490767 OS=Cucurbita maxima OX=3661 GN=LOC111490767... [more]
A0A6J1DGD31.1e-11786.78uncharacterized protein LOC111020607 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A6J1FXD44.8e-11385.89uncharacterized protein LOC111448431 OS=Cucurbita moschata OX=3662 GN=LOC1114484... [more]
A0A6J1JFP26.1e-10884.23uncharacterized protein LOC111484597 OS=Cucurbita maxima OX=3661 GN=LOC111484597... [more]
Match NameE-valueIdentityDescription
AT5G19430.12.1e-6856.73RING/U-box superfamily protein [more]
AT5G19430.24.0e-6757.74RING/U-box superfamily protein [more]
AT5G12310.11.0e-6255.97RING/U-box superfamily protein [more]
AT3G30460.11.3e-0444.90RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 46..88
e-value: 7.2E-6
score: 35.5
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 46..89
e-value: 1.1E-8
score: 35.3
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 46..89
score: 12.592549
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 22..94
e-value: 4.1E-12
score: 47.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 188..243
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 199..243
NoneNo IPR availablePANTHERPTHR47361:SF4RING/U-BOX SUPERFAMILY PROTEINcoord: 11..243
NoneNo IPR availablePANTHERPTHR47361RING/U-BOX SUPERFAMILY PROTEINcoord: 11..243
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 40..96
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 64..73

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg02689-RACarg02689-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
molecular_function GO:0016301 kinase activity
molecular_function GO:0046872 metal ion binding