Carg01665 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg01665
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Mitochondrial glycoprotein
Location	Carg_Chr15: 2684530 .. 2687347 (-)
RNA-Seq Expression	Carg01665
Synteny	Carg01665

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATAGAAAATTTGACTTTGCTTCAAGGTACATTTTTATTGGTATTTCAAACTAGAAATTACCGCACAAATCTTTTTTTTAAAGAAATAAACGGAGCTTTTATGTACTAAAAATAAATCCAAAATACCGAAATTATATTTTAAATTTTTCATGTGGCCGGTCTAACTCATTGACCAAGTTTGCATCTGCAGACCGTCTCGAACCGCTTCGGTCGGACCGGTAAATTATGCTTTAAAACCGCGGCAAAGTCTCAAGTTCAACAACACACAACCTCTACAGCAAAATCGAAGGAAAATGTTGAAGAAAGTGACGGCCGTCGCTGCGGTGGCTTTACGGGGTAGCCACCGGCCGTCTCTCATGGCCACTTGCCGCCATTCGACCTCCGTGTCTACTGCCGTCAATTCATTGCTCCTCCGCTCCCTCAAAGAACACTATCTCGAGATCTCCAGAATGACGCCTCCTCCTGTAAGGTTTCTATTGCTTTGTCCTCTGTTTTCAATTGCTAAACCCTAGAATTAGGGTTTTAAGCTATACGAAGATCGTTTGTTCATCTTACTCGGTTTTCTCCTTTTTATCGCAGAAAGTGAGCCCTCCGTCTTCCTTTTCTATCATTAAAGGCGCACTAGACGGCAATGGTCCGGTGCTTACTAGGAGCTACGGCAATGAAGAGATTAACGTTTCGGTTATGCGATTGGCTAATATTGTTCCGGGAGGCGGTGGAGACGATGGAGACGACGAAATTAACCAGTTGTTTCTTCATCTGACTGTATCAAAGCCGGAGCAGAGGGAAAGTTTACATTTTCTTTGTGGATTGTATCCCGATGCATTAGGTATTCATTCCGTTTCGATGAGACCAAAAGCTGAATCCTCGGGGTTTCTCGTCGTCCCGTCTGGCTATAATGGTCCCGTGTTCGAGTAAGTTTTATGTCTTTAATCCAGAGAGTGTTGGAACAATTTTATTTAATCGGCATGGCTTCCATATCTCTGCAGGACATGGTACCATTTTAAAGTAAGTTAGCCTCAATTACTATGTAGTCTGTTGCCTCATGTTTAGTGGTAGTTAACATTTAGTCAAGTAGTTCAAGCAATGTTGTTCATGGCACCAATTAGAGAAATGCTTATAATTTGGATCCCATGTCTCAATTTTTAGTGTTATCATCTAAACTAACAAAAACTGAATCATTTTCTCCTAGTAAAGTAATCGAAGGAGTTAGATCTAAGTAAAGGTCATGAGTTCAGGATTTGTTGCTGCATACAATTTGCTGCCAAGAATCTGTAATTTAAACAACACGACCCATTCGGTAATGAACTATTCTGGGTTGCTAGTTTTGGTTCGCAGGTTAAAGATATCAGTTAACTTTCTTTAGTATATATTGTTTATTGCCTAAGGAGCTTATCATTCTAGTCTCCACTGTTTGAATTCTTCATTGCAGCTACCTATTTTGTTCCTTAACTTGCAGGCTGCAGCCTCTGATTTCTTTTCTTTGGTTACGAACTTAGACTTCGAGATTTAATTTTTCCACTTGCAGTTGATTGAATCCCTTATATCATGTCACTCACTTGGGATGCTTGCAATTAGCACCCAGCATCATAGAATAGTGCAAATTTGAAGTGATACTGTAGTTCATATTGTTACTCTGCTTGGATCATTGGCTAAAGCGAAATTTCCCAATGGGTTAGGGCATCCCCTAAGTAAGCCAACCTGGATAGCAACGTGTAAGAGAATATGTATCGAATCCAATATATATATACTTCGCCTTTCTTGTGTTAAAACACTTAGTAAGTTAGTACCCTTTGGTTTGGTTGAGTAGCTCTCTTTGCTCTGATTTGGCTTGTGTTCTTGTATGGCCTAGTTCTATAATCCTTTATATTTATTTTTATGAAAGCTATCTCTTACTAAATAGTTTCTTTTTCATCATAGTTGGCCTAGATATGGTGTCGAAGGGTCATGATGGTAGTTCTTCTTTTCATAGCATATATGTCAGGTTTTTTATTGAACCAAGTGGGATTGTGATGTTGAGCCTTATGATTTTCATATGCTTATAATCTCGACCAAATTACTGTTGATGGGAAATCATAAGAAAACTCACACTTATGTTTCTTTGTATAACAGAGATCTGGATGAGAAAATGCGAGATGTATTTCACAATTACATAGAGGAGCGAGGTATAAACGAGTCTCTCTTTCCATTTCTTCAGGCATGGCTTTATGTGAAAGAGCATCGAAATCTTATGCGGTGGTTCAGATCCGTTGGCTCGTTCATCAACGAGCGCAAGAACGCTAAAGAGGATTGATAGTTCATGTATTGGATAGGAAAATATGTGGAATTCAAGGTTCCATCTTCATAATTGGTTAGTTTTATAAGGTAAATGGTCTTATTTCATTTTAAGATACAATTTTGCAAGCCCCTATGTGGTGATTCGTCTCTCCTTGTTAGCCATGGTTTTTGTTTTCCAAAGTTGATTAACGGTGCTATATATTGCAGCTTTATTCTTATGGATTTTCTTCTTATACCTTCGAAAGTTGTAGTTAAATTTTGTTATCTACACTCAAATGGATGCATTTCTCGAGTACCGACCCTCGTTCAAATTCTCATTTCGTTAACGATTTGAGAAATATACTCTGGACCCATATTAGAATTACTGAAATCCCATGTCTAGCTCGAGAATTTCTAATACAACAGATGTGTTACTATTCTCTATTTCTCTGAATTGTTGTAGGAACCTGTTGGAATAACATGATCGGTAGTTCTTGAATCAGTATTAGGAGGCATACGGATCTCAAAATTTTATCTAATGAGAGCCAATTAGTCATATTGT

mRNA sequence

TATAGAAAATTTGACTTTGCTTCAAGGTACATTTTTATTGGTATTTCAAACTAGAAATTACCGCACAAATCTTTTTTTTAAAGAAATAAACGGAGCTTTTATGTACTAAAAATAAATCCAAAATACCGAAATTATATTTTAAATTTTTCATGTGGCCGGTCTAACTCATTGACCAAGTTTGCATCTGCAGACCGTCTCGAACCGCTTCGGTCGGACCGGTAAATTATGCTTTAAAACCGCGGCAAAGTCTCAAGTTCAACAACACACAACCTCTACAGCAAAATCGAAGGAAAATGTTGAAGAAAGTGACGGCCGTCGCTGCGGTGGCTTTACGGGGTAGCCACCGGCCGTCTCTCATGGCCACTTGCCGCCATTCGACCTCCGTGTCTACTGCCGTCAATTCATTGCTCCTCCGCTCCCTCAAAGAACACTATCTCGAGATCTCCAGAATGACGCCTCCTCCTAAAGTGAGCCCTCCGTCTTCCTTTTCTATCATTAAAGGCGCACTAGACGGCAATGGTCCGGTGCTTACTAGGAGCTACGGCAATGAAGAGATTAACGTTTCGGTTATGCGATTGGCTAATATTGTTCCGGGAGGCGGTGGAGACGATGGAGACGACGAAATTAACCAGTTGTTTCTTCATCTGACTGTATCAAAGCCGGAGCAGAGGGAAAGTTTACATTTTCTTTGTGGATTGTATCCCGATGCATTAGGTATTCATTCCGTTTCGATGAGACCAAAAGCTGAATCCTCGGGGTTTCTCGTCGTCCCGTCTGGCTATAATGGTCCCGTGTTCGAAGATCTGGATGAGAAAATGCGAGATGTATTTCACAATTACATAGAGGAGCGAGGTATAAACGAGTCTCTCTTTCCATTTCTTCAGGCATGGCTTTATGTGAAAGAGCATCGAAATCTTATGCGGTGGTTCAGATCCGTTGGCTCGTTCATCAACGAGCGCAAGAACGCTAAAGAGGATTGATAGTTCATGTATTGGATAGGAAAATATGTGGAATTCAAGGTTCCATCTTCATAATTGGTTAGTTTTATAAGGAACCTGTTGGAATAACATGATCGGTAGTTCTTGAATCAGTATTAGGAGGCATACGGATCTCAAAATTTTATCTAATGAGAGCCAATTAGTCATATTGT

Coding sequence (CDS)

ATGTTGAAGAAAGTGACGGCCGTCGCTGCGGTGGCTTTACGGGGTAGCCACCGGCCGTCTCTCATGGCCACTTGCCGCCATTCGACCTCCGTGTCTACTGCCGTCAATTCATTGCTCCTCCGCTCCCTCAAAGAACACTATCTCGAGATCTCCAGAATGACGCCTCCTCCTAAAGTGAGCCCTCCGTCTTCCTTTTCTATCATTAAAGGCGCACTAGACGGCAATGGTCCGGTGCTTACTAGGAGCTACGGCAATGAAGAGATTAACGTTTCGGTTATGCGATTGGCTAATATTGTTCCGGGAGGCGGTGGAGACGATGGAGACGACGAAATTAACCAGTTGTTTCTTCATCTGACTGTATCAAAGCCGGAGCAGAGGGAAAGTTTACATTTTCTTTGTGGATTGTATCCCGATGCATTAGGTATTCATTCCGTTTCGATGAGACCAAAAGCTGAATCCTCGGGGTTTCTCGTCGTCCCGTCTGGCTATAATGGTCCCGTGTTCGAAGATCTGGATGAGAAAATGCGAGATGTATTTCACAATTACATAGAGGAGCGAGGTATAAACGAGTCTCTCTTTCCATTTCTTCAGGCATGGCTTTATGTGAAAGAGCATCGAAATCTTATGCGGTGGTTCAGATCCGTTGGCTCGTTCATCAACGAGCGCAAGAACGCTAAAGAGGATTGA

Protein sequence

MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVSPPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTVSKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFHNYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED

Homology

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BLAST of Carg01665 vs. NCBI nr
Match: XP_022938797.1 (uncharacterized protein At2g39795, mitochondrial [Cucurbita moschata] >KAG6578831.1 putative protein, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016357.1 putative protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 461.1 bits (1185), Expect = 5.7e-126
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60
           MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS
Sbjct: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60

Query: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120
           PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV
Sbjct: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120

Query: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFH 180
           SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFH
Sbjct: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFH 180

Query: 181 NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 229
           NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED
Sbjct: 181 NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 228

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BLAST of Carg01665 vs. NCBI nr
Match: XP_022992760.1 (uncharacterized protein At2g39795, mitochondrial [Cucurbita maxima])

HSP 1 Score: 449.5 bits (1155), Expect = 1.7e-122
Identity = 222/228 (97.37%), Postives = 225/228 (98.68%), Query Frame = 0


Query: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60
           MLKKVTAVAAVALRGSHRPSLMATCRHST+VSTAVNSLLLRSLKEHYLEISRMTPPPKVS
Sbjct: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTTVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60

Query: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120
           PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLH+TV
Sbjct: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHVTV 120

Query: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFH 180
           SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDV H
Sbjct: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVLH 180

Query: 181 NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 229
           NYIEERGIN+SLF FLQAWLYVKEHRNLMRWFRSVGSFINE KNAKED
Sbjct: 181 NYIEERGINQSLFSFLQAWLYVKEHRNLMRWFRSVGSFINEPKNAKED 228

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BLAST of Carg01665 vs. NCBI nr
Match: XP_023549717.1 (uncharacterized protein At2g39795, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 447.2 bits (1149), Expect = 8.5e-122
Identity = 223/228 (97.81%), Postives = 223/228 (97.81%), Query Frame = 0


Query: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60
           MLKKVTAVAAVALRGS RPSLMATCR STSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS
Sbjct: 1   MLKKVTAVAAVALRGSRRPSLMATCRRSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60

Query: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120
           PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV
Sbjct: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120

Query: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFH 180
           SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDV H
Sbjct: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVLH 180

Query: 181 NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 229
           NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINE KNAK D
Sbjct: 181 NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINEPKNAKVD 228

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BLAST of Carg01665 vs. NCBI nr
Match: XP_038884404.1 (uncharacterized protein At2g39795, mitochondrial [Benincasa hispida])

HSP 1 Score: 420.6 bits (1080), Expect = 8.5e-114
Identity = 206/228 (90.35%), Postives = 216/228 (94.74%), Query Frame = 0


Query: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60
           MLKK  A  AVALR S RPSLM TCRHSTSV++AVNSLLLRSLKEHYLEISRMTPPPKVS
Sbjct: 1   MLKKAMAATAVALR-SRRPSLMVTCRHSTSVTSAVNSLLLRSLKEHYLEISRMTPPPKVS 60

Query: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120
           PPSSFSII+GALDGNGPVLTR+YG EE+ +SVMRLANIVPGGGGDDGDDEINQLFLH+TV
Sbjct: 61  PPSSFSIIQGALDGNGPVLTRNYGKEEVKISVMRLANIVPGGGGDDGDDEINQLFLHVTV 120

Query: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFH 180
           SK EQ++ LHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRD+FH
Sbjct: 121 SKLEQKDDLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDIFH 180

Query: 181 NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 229
           NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINE KNAKED
Sbjct: 181 NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINEHKNAKED 227

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BLAST of Carg01665 vs. NCBI nr
Match: XP_022134253.1 (uncharacterized protein At2g39795, mitochondrial [Momordica charantia])

HSP 1 Score: 407.5 bits (1046), Expect = 7.4e-110
Identity = 200/230 (86.96%), Postives = 216/230 (93.91%), Query Frame = 0


Query: 1   MLKKVTAVAAVALRGSHRPSLMATC-RHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKV 60
           M+KK+ AVAA AL+   RPSLM TC RHSTSVS+AVNSLLLRSLKEHYLEISRMTPPPKV
Sbjct: 1   MMKKLIAVAAEALQSRRRPSLMTTCRRHSTSVSSAVNSLLLRSLKEHYLEISRMTPPPKV 60

Query: 61  SPPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGG-DDGDDEINQLFLHL 120
           SPPS FSIIKGALD NGPVLTR++G EE+N+SVMRLANIVPGGGG DDGD+EINQLFLH+
Sbjct: 61  SPPSPFSIIKGALDSNGPVLTRNFGEEEVNISVMRLANIVPGGGGDDDGDEEINQLFLHV 120

Query: 121 TVSKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDV 180
           TVSKPEQR++LHFLCGLYPDALGIHSVSMRP+AESSGFLVVPSGYNGPVFEDLDEKMRDV
Sbjct: 121 TVSKPEQRDNLHFLCGLYPDALGIHSVSMRPRAESSGFLVVPSGYNGPVFEDLDEKMRDV 180

Query: 181 FHNYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 229
            HNYI+ERG+NESLFPFLQAWLYVKEHRNLMRWFRS GSFINERK AKE+
Sbjct: 181 LHNYIDERGVNESLFPFLQAWLYVKEHRNLMRWFRSAGSFINERKGAKEN 230

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BLAST of Carg01665 vs. ExPASy Swiss-Prot
Match: Q8W487 (Uncharacterized protein At2g39795, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g39795 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 1.1e-12
Identity = 62/233 (26.61%), Postives = 107/233 (45.92%), Query Frame = 0


Query: 4   KVTAVAAVALR-------GSHRPSLMATCRHSTSVS-TAVNSLLLRSLKEHY---LEISR 63
           +V +++AVA R        S RP +     +ST++   +    L+R +       L+   
Sbjct: 22  RVRSISAVANRASLARNPSSIRPFVSRALNYSTAIDRISSEQTLIRVIDSEINSALQSDN 81

Query: 64  MTPPPKVSPPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDE-- 123
           +    +++P S    I+         LTR Y  E I V V   + +      DD DDE  
Sbjct: 82  IDSDEEMTPGSFPFRIEDKPGNQNVTLTRDYNGEHIKVVVSMPSLVSDENDDDDDDDEGP 141

Query: 124 --INQLFLHLTVSKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVF 183
              + + L +TV+K +   +L F C  +PD + I ++S++   +S   L       GP F
Sbjct: 142 SNESSIPLVVTVTK-KSGLTLEFSCMAFPDEIAIDALSVKHPGDS---LEDQLANEGPDF 201

Query: 184 EDLDEKMRDVFHNYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINE 222
           EDLDE ++  F+ ++E RG+  S   FL  ++  K +R    W ++V  F+ +
Sbjct: 202 EDLDENLKKTFYKFLEIRGVKASTTNFLHEYMTRKVNREYFLWLKNVKEFMEQ 250

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BLAST of Carg01665 vs. ExPASy Swiss-Prot
Match: P40513 (Mitochondrial acidic protein MAM33 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MAM33 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.9e-07
Identity = 46/206 (22.33%), Postives = 85/206 (41.26%), Query Frame = 0


Query: 48  LEISRMTPPPKVSPPS--------SFSIIKGALDGNGPVLTRSYGNEEINV--SVMRLAN 107
           L+I + T P   S  S         FS+++        ++ R+   E ++V   V ++AN
Sbjct: 61  LKIEKETLPESTSLDSFNDFLNKYKFSLVETPGKNEAEIVRRTESGETVHVFFDVAQIAN 120

Query: 108 IV------------PGGGGDDGDDEINQLFLHLTV-----SKPEQRESLHFLCGLYPDAL 167
           +               G  +D  D ++  F ++ V     S  E   S   L  L   + 
Sbjct: 121 LPYNNAMDENTEQNEDGINEDDFDALSDNFANVNVVISKESASEPAVSFELLMNLQEGSF 180

Query: 168 GIHSVSMRPKAESS------GFLVVPSGYNGPVFEDLDEKMRDVFHNYIEERGINESLFP 221
            + S +  P  +++        +     Y+GP F +LDE++++    Y+E RG+NE L  
Sbjct: 181 YVDSATPYPSVDAALNQSAEAEITRELVYHGPPFSNLDEELQESLEAYLESRGVNEELAS 240

[Close]

BLAST of Carg01665 vs. ExPASy Swiss-Prot
Match: O94675 (Mitochondrial acidic protein mam33 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPBC776.07 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.2e-07
Identity = 24/57 (42.11%), Postives = 39/57 (68.42%), Query Frame = 0


Query: 163 YNGPVFEDLDEKMRDVFHNYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFI 220
           Y GP F++LD +++D+FH+Y+EER I+ESL  F+ ++   KE +  + W  SV  F+
Sbjct: 212 YLGPSFKELDPELQDLFHSYLEERKIDESLSSFIVSFGLTKELKEYINWLESVRQFL 268

[Close]

BLAST of Carg01665 vs. ExPASy TrEMBL
Match: A0A6J1FF36 (uncharacterized protein At2g39795, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111444904 PE=4 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 2.7e-126
Identity = 228/228 (100.00%), Postives = 228/228 (100.00%), Query Frame = 0


Query: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60
           MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS
Sbjct: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60

Query: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120
           PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV
Sbjct: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120

Query: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFH 180
           SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFH
Sbjct: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFH 180

Query: 181 NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 229
           NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED
Sbjct: 181 NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 228

[Close]

BLAST of Carg01665 vs. ExPASy TrEMBL
Match: A0A6J1JWM0 (uncharacterized protein At2g39795, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111489000 PE=4 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 8.3e-123
Identity = 222/228 (97.37%), Postives = 225/228 (98.68%), Query Frame = 0


Query: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60
           MLKKVTAVAAVALRGSHRPSLMATCRHST+VSTAVNSLLLRSLKEHYLEISRMTPPPKVS
Sbjct: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTTVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60

Query: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120
           PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLH+TV
Sbjct: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHVTV 120

Query: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFH 180
           SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDV H
Sbjct: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVLH 180

Query: 181 NYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 229
           NYIEERGIN+SLF FLQAWLYVKEHRNLMRWFRSVGSFINE KNAKED
Sbjct: 181 NYIEERGINQSLFSFLQAWLYVKEHRNLMRWFRSVGSFINEPKNAKED 228

[Close]

BLAST of Carg01665 vs. ExPASy TrEMBL
Match: A0A6J1C1F6 (uncharacterized protein At2g39795, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111006555 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.6e-110
Identity = 200/230 (86.96%), Postives = 216/230 (93.91%), Query Frame = 0


Query: 1   MLKKVTAVAAVALRGSHRPSLMATC-RHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKV 60
           M+KK+ AVAA AL+   RPSLM TC RHSTSVS+AVNSLLLRSLKEHYLEISRMTPPPKV
Sbjct: 1   MMKKLIAVAAEALQSRRRPSLMTTCRRHSTSVSSAVNSLLLRSLKEHYLEISRMTPPPKV 60

Query: 61  SPPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGG-DDGDDEINQLFLHL 120
           SPPS FSIIKGALD NGPVLTR++G EE+N+SVMRLANIVPGGGG DDGD+EINQLFLH+
Sbjct: 61  SPPSPFSIIKGALDSNGPVLTRNFGEEEVNISVMRLANIVPGGGGDDDGDEEINQLFLHV 120

Query: 121 TVSKPEQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDV 180
           TVSKPEQR++LHFLCGLYPDALGIHSVSMRP+AESSGFLVVPSGYNGPVFEDLDEKMRDV
Sbjct: 121 TVSKPEQRDNLHFLCGLYPDALGIHSVSMRPRAESSGFLVVPSGYNGPVFEDLDEKMRDV 180

Query: 181 FHNYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 229
            HNYI+ERG+NESLFPFLQAWLYVKEHRNLMRWFRS GSFINERK AKE+
Sbjct: 181 LHNYIDERGVNESLFPFLQAWLYVKEHRNLMRWFRSAGSFINERKGAKEN 230

[Close]

BLAST of Carg01665 vs. ExPASy TrEMBL
Match: A0A0A0KCG3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G046350 PE=4 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 3.9e-104
Identity = 191/230 (83.04%), Postives = 207/230 (90.00%), Query Frame = 0


Query: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60
           MLKK+ A +AVALR   RPSLM TCRHSTS S+AVNS LLRSLKEHYLEI+RM  PPKVS
Sbjct: 1   MLKKMMAASAVALRSGTRPSLMFTCRHSTSASSAVNSFLLRSLKEHYLEITRMAAPPKVS 60

Query: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120
           PPSSFSII GALDGNGPVLT++YG EEIN+SVMRLANIV GGGG D DDEINQLFLH+TV
Sbjct: 61  PPSSFSIIVGALDGNGPVLTKNYGKEEINISVMRLANIVRGGGGGDDDDEINQLFLHVTV 120

Query: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAES--SGFLVVPSGYNGPVFEDLDEKMRDV 180
           SK EQ+++LHFLCGLYPDALGIHSVSMRPKAES  SGFLVVPS YNGP FEDLDEKMRD+
Sbjct: 121 SKLEQKDNLHFLCGLYPDALGIHSVSMRPKAESSGSGFLVVPSSYNGPTFEDLDEKMRDM 180

Query: 181 FHNYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 229
           FHNYIEERG+NESLFPFLQAWLYVKEHRNL+RWFRSVG+FI E KN K+D
Sbjct: 181 FHNYIEERGVNESLFPFLQAWLYVKEHRNLLRWFRSVGTFIYEPKNGKKD 230

[Close]

BLAST of Carg01665 vs. ExPASy TrEMBL
Match: A0A1S3C3N7 (uncharacterized protein At2g39795, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103496527 PE=4 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.6e-102
Identity = 183/230 (79.57%), Postives = 206/230 (89.57%), Query Frame = 0


Query: 1   MLKKVTAVAAVALRGSHRPSLMATCRHSTSVSTAVNSLLLRSLKEHYLEISRMTPPPKVS 60
           ML K+ A  AVALR   RPSLM TCRHSTS S+AVNS LLRSLKEHYLEI+RM  PPKVS
Sbjct: 1   MLNKLMAATAVALRSGSRPSLMVTCRHSTSASSAVNSFLLRSLKEHYLEITRMPAPPKVS 60

Query: 61  PPSSFSIIKGALDGNGPVLTRSYGNEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTV 120
           PPSSFSII GALDG+GPVLT++YG EE+N+SVMRL+NIV GGGGD+ DD+INQLFLH++V
Sbjct: 61  PPSSFSIITGALDGHGPVLTKNYGKEEVNISVMRLSNIVRGGGGDNDDDDINQLFLHVSV 120

Query: 121 SKPEQRESLHFLCGLYPDALGIHSVSMRPKAES--SGFLVVPSGYNGPVFEDLDEKMRDV 180
           SKPEQ+++LHFLCGLYPDALGIHSVSMRPK ES  SGFL+VPS YNGP FEDLDEKMRD+
Sbjct: 121 SKPEQKDTLHFLCGLYPDALGIHSVSMRPKGESSGSGFLLVPSSYNGPTFEDLDEKMRDM 180

Query: 181 FHNYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINERKNAKED 229
           FHNYI+ERG+NESLFPFLQAWLYVKEHRNL+RWFRSVG+FI E KN K+D
Sbjct: 181 FHNYIDERGVNESLFPFLQAWLYVKEHRNLLRWFRSVGTFIYEPKNGKKD 230

[Close]

BLAST of Carg01665 vs. TAIR 10
Match: AT4G32605.1 (Mitochondrial glycoprotein family protein )

HSP 1 Score: 285.0 bits (728), Expect = 5.2e-77
Identity = 137/200 (68.50%), Postives = 167/200 (83.50%), Query Frame = 0


Query: 28  STSVSTAVNSLLLRSLKEHYLEISRMTPPPKVSPPSSFSIIKGALDGN-GPVLTRSYGNE 87
           S +VS AV+S+LLRSLKEHYLE+S+MTPPPKVSPPS F I+KG+L+G+ G VL +S GNE
Sbjct: 29  SANVSAAVDSMLLRSLKEHYLEVSKMTPPPKVSPPSPFEIVKGSLEGSTGAVLKKSVGNE 88

Query: 88  EINVSVMRLANIVPGGGGDDGDDEINQLFLHLTVSKPEQRESLHFLCGLYPDALGIHSVS 147
           EIN+ VMRLA+    GG ++ D  INQLFLH+ VSKP Q +SLHFLCGLYPDALGIHSVS
Sbjct: 89  EINLFVMRLAH----GGDEEDDGGINQLFLHVAVSKPNQPDSLHFLCGLYPDALGIHSVS 148

Query: 148 MRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFHNYIEERGINESLFPFLQAWLYVKEHR 207
           MRPK E+      P+ Y GP FE+LDEKMRDVFH ++EERG+NESLFPFLQAWLYVK+HR
Sbjct: 149 MRPKLEALEMSDDPTQYTGPSFEELDEKMRDVFHGFLEERGVNESLFPFLQAWLYVKDHR 208

Query: 208 NLMRWFRSVGSFINERKNAK 227
           NL+RWF+SVG+F++E  +A+
Sbjct: 209 NLLRWFKSVGTFVHENPSAE 224

[Close]

BLAST of Carg01665 vs. TAIR 10
Match: AT1G80720.1 (Mitochondrial glycoprotein family protein )

HSP 1 Score: 84.0 bits (206), Expect = 1.8e-16
Identity = 55/191 (28.80%), Postives = 93/191 (48.69%), Query Frame = 0


Query: 39  LLRSLKEHYLEISRMTPPPKVSPPSSFS--IIKGALDGNGPVLTRSYGN-EEINVSVMRL 98
           L+R+  E+ L+ S     P + PP+SF    +          L R++G+ E+I +     
Sbjct: 13  LIRNEIEYELDHS-----PPLQPPNSFGPFTVDERPGEQWISLKRNFGDKEDIKIEATMF 72

Query: 99  ANIVPGGGGDDGDDEINQLFLHLT----VSKPEQRESLHFLCGLYPDALGIHSVSMRPKA 158
              VP       + E     LH+T    +SK    E+L  +C  +PD + I  + +R   
Sbjct: 73  DRSVPTSKSTKTEPE---YILHITFIVNISKAGATEALEIMCSAWPDTIEISKLCIR--- 132

Query: 159 ESSGFLVVPSGYNGPVFEDLDEKMRDVFHNYIEERGINESLFPFLQAWLYVKEHRNLMRW 218
              G    PS Y GP FE+LD++++D  + ++EERGI++ L  FL  ++  K     +RW
Sbjct: 133 --RGINTSPSSYGGPEFEELDDQLQDALYQFLEERGISDELAVFLHRYMKNKGKAEYVRW 190

Query: 219 FRSVGSFINER 223
             SV S++ ++
Sbjct: 193 MESVKSYVEQK 190

[Close]

BLAST of Carg01665 vs. TAIR 10
Match: AT3G55605.1 (Mitochondrial glycoprotein family protein )

HSP 1 Score: 79.7 bits (195), Expect = 3.3e-15
Identity = 49/158 (31.01%), Postives = 79/158 (50.00%), Query Frame = 0


Query: 79  LTRSYGNEEINVSVMRLANIVPGGGGDDGDDEI---------------NQLFLHLTVSKP 138
           LTR Y  E+I V V      +PG   D+ +D++               + + L +TV+K 
Sbjct: 110 LTREYNGEQIKVEVS-----MPGLAMDENEDDVDDDEDGDGRHEKSNESSIPLVVTVTK- 169

Query: 139 EQRESLHFLCGLYPDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFHNYI 198
           +   SL F C  +PD + I  +S+    +SS   +    Y+GP F++LDE MR  FH ++
Sbjct: 170 KSGLSLEFSCTAFPDEIVIDGLSVNRPDDSSEEQLT---YDGPDFQELDENMRKSFHKFL 229

Query: 199 EERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFINE 222
           E RGI  S   FL  ++  K+ R  + W + + +F+ E
Sbjct: 230 ETRGIKASATDFLYEYMMKKDSREYLLWLKKLKTFVQE 258

[Close]

BLAST of Carg01665 vs. TAIR 10
Match: AT5G05990.1 (Mitochondrial glycoprotein family protein )

HSP 1 Score: 79.3 bits (194), Expect = 4.3e-15
Identity = 54/160 (33.75%), Postives = 76/160 (47.50%), Query Frame = 0


Query: 79  LTRSYGNEEINVSVMRLANIVPGGGGDDGDDE------------------INQLFLHLTV 138
           LTR Y  E + V V  + N+V G   DD DDE                   + + L +T+
Sbjct: 104 LTREYEGETVKVEV-HMTNLVTGDKEDDEDDEEEAENEEDEDEDKPLKPKQSNVPLLVTL 163

Query: 139 SKPEQRESLHFLCGLYPDALGIHSVSMR-PKAESSGFLVVPSGYNGPVFEDLDEKMRDVF 198
           SK +   SL F C  +PD + I  + +  P   S   L     Y GP F  LDEK+R  F
Sbjct: 164 SK-KTGPSLEFRCTAFPDRIAIKDMWVTFPDDPSKDEL----AYEGPSFRVLDEKLRKAF 223

Query: 199 HNYIEERGINESLFPFLQAWLYVKEHRNLMRWFRSVGSFI 220
           H YIE RGI  S+  FL  ++  K+ R  + W +S+ +F+
Sbjct: 224 HRYIEIRGIKPSMINFLHEYMINKDSREHLLWLKSLKNFV 257

[Close]

BLAST of Carg01665 vs. TAIR 10
Match: AT1G15870.1 (Mitochondrial glycoprotein family protein )

HSP 1 Score: 77.4 bits (189), Expect = 1.6e-14
Identity = 39/146 (26.71%), Postives = 75/146 (51.37%), Query Frame = 0


Query: 79  LTRSYG-NEEINVSVMRLANIVPGGGGDDGDDEINQLFLHLTVS-KPEQRESLHFLCGLY 138
           L R +G  E+I +        VP       D E  QL +   V+      E+L  +C  +
Sbjct: 102 LRRKFGEKEDIKIEATMFDGSVPSSKSTTSDPEDVQLHITFIVNISKGDGETLEIMCSAW 161

Query: 139 PDALGIHSVSMRPKAESSGFLVVPSGYNGPVFEDLDEKMRDVFHNYIEERGINESLFPFL 198
           PD + I    +R  +++S     P+ Y GP F++++++++D  + ++EERGI++ L  FL
Sbjct: 162 PDTIQITKFFVRKSSQNS-----PNAYIGPEFQEMEDELQDSVYRFLEERGISDDLAEFL 221

Query: 199 QAWLYVKEHRNLMRWFRSVGSFINER 223
             ++  K+    +RW  +V S++ ++
Sbjct: 222 HQYMKNKDKAEYIRWMETVKSYVEQK 242

The following BLAST results are available for this feature:

BLAST of Carg01665 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022938797.1	5.7e-126	100.00	uncharacterized protein At2g39795, mitochondrial [Cucurbita moschata] >KAG657883...	[more]
XP_022992760.1	1.7e-122	97.37	uncharacterized protein At2g39795, mitochondrial [Cucurbita maxima]	[more]
XP_023549717.1	8.5e-122	97.81	uncharacterized protein At2g39795, mitochondrial [Cucurbita pepo subsp. pepo]	[more]
XP_038884404.1	8.5e-114	90.35	uncharacterized protein At2g39795, mitochondrial [Benincasa hispida]	[more]
XP_022134253.1	7.4e-110	86.96	uncharacterized protein At2g39795, mitochondrial [Momordica charantia]	[more]

BLAST of Carg01665 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8W487	1.1e-12	26.61	Uncharacterized protein At2g39795, mitochondrial OS=Arabidopsis thaliana OX=3702...	[more]
P40513	1.9e-07	22.33	Mitochondrial acidic protein MAM33 OS=Saccharomyces cerevisiae (strain ATCC 2045...	[more]
O94675	4.2e-07	42.11	Mitochondrial acidic protein mam33 OS=Schizosaccharomyces pombe (strain 972 / AT...	[more]

BLAST of Carg01665 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FF36	2.7e-126	100.00	uncharacterized protein At2g39795, mitochondrial OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1JWM0	8.3e-123	97.37	uncharacterized protein At2g39795, mitochondrial OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1C1F6	3.6e-110	86.96	uncharacterized protein At2g39795, mitochondrial OS=Momordica charantia OX=3673 ...	[more]
A0A0A0KCG3	3.9e-104	83.04	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G046350 PE=4 SV=1	[more]
A0A1S3C3N7	3.6e-102	79.57	uncharacterized protein At2g39795, mitochondrial OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of Carg01665 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G32605.1	5.2e-77	68.50	Mitochondrial glycoprotein family protein	[more]
AT1G80720.1	1.8e-16	28.80	Mitochondrial glycoprotein family protein	[more]
AT3G55605.1	3.3e-15	31.01	Mitochondrial glycoprotein family protein	[more]
AT5G05990.1	4.3e-15	33.75	Mitochondrial glycoprotein family protein	[more]
AT1G15870.1	1.6e-14	26.71	Mitochondrial glycoprotein family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003428	Mitochondrial glycoprotein	PFAM	PF02330	MAM33	coord: 78..219 e-value: 1.7E-22 score: 80.5
IPR036561	Mitochondrial glycoprotein superfamily	GENE3D	3.10.280.10	Mitochondrial glycoprotein	coord: 37..219 e-value: 5.2E-31 score: 109.9
IPR036561	Mitochondrial glycoprotein superfamily	SUPERFAMILY	54529	Mitochondrial glycoprotein MAM33-like	coord: 41..220
None	No IPR available	PANTHER	PTHR31365	EXPRESSED PROTEIN	coord: 1..224
None	No IPR available	PANTHER	PTHR31365:SF2	MITOCHONDRIAL GLYCOPROTEIN FAMILY PROTEIN-LIKE	coord: 1..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg01665-RA	Carg01665-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005759	mitochondrial matrix