Carg01299 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg01299
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionWAT1-related protein
LocationCarg_Chr06: 136443 .. 137419 (+)
RNA-Seq ExpressionCarg01299
SyntenyCarg01299
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATTCATAGAATGCCTTGGCTGTTTCTTTCAAAATGCAAGGCCTTACATCGCAGTCATCTCCCTCCAATTCGGCTACGCCGGGATGAACATCATCTCCGTCGTCTCCCTCAACCGTGGTATGAGCCATTACGTCCTCGTTGTCTACCGCCACGCCTTTGCCACCGCTGTCATGGCTCCTTTTGCTCTCGTTCTCGAGAGGTACATATCTTTGAGATTCATTCTTACTTATATAACAATTCTCATTTCTTACTCACGTAGTACTATACTATTCATGGTTATTGTTTCGTAGGAAAGTAAGACCCAAGATTACATGGAAGATTTTCATTCAGATGTTTGTTCTCGCTCTTCTTGGGTTAGTATATCAAAACTCGATAGTTTTTCCAAACATCCATTAATCCGATCTTTTGTATATTTCATAACATCGATCTCAACAGGCCACTGATCGATCAGAATTTCTACTACGTCGGGCTGAAAATGACATCCCCAACGTACTCGTGTGCCATAAGCAACATGCTTCCTTCGATGACATTCATCATGGCAGTCCTTTGCAGGATGGAGAAGCTGGACTTGAGGAAAGTGAGATGCCAAGCCAAGTTGTTTGGAACAATAGTGACGGTGATGGGAGCCATGTTGATGACATTTTACAAAGGAAGTGTCATCAACTTCTTCTGGACCGAGCAAGCCCACCACCCGAACTCACCACCGACAGCGGATGCGGCTGCGCTCAACCACCACCACGACGGAGAGTTCATCAAAGGCTCAATTTTGCTCATCATAGCCACTCTAGCTTGGGCGGGGTTCTTCATTCTCCAAGTCATAACCTTGAGAAAATACACCGCCCATCTTTCTCTCACAACCATGGTTTGCTTTTTGGGAACCCTTCAGGCCATTGTTGTCACCCTCGCTATGGAACACCGCCCCTCCGCCTGGGCCATCGGCTGGGACATGAACCTTCTCGCCGCCGCTTATGCG

mRNA sequence

ATGGGATTCATAGAATGCCTTGGCTGTTTCTTTCAAAATGCAAGGCCTTACATCGCAGTCATCTCCCTCCAATTCGGCTACGCCGGGATGAACATCATCTCCGTCGTCTCCCTCAACCGTGGTATGAGCCATTACGTCCTCGTTGTCTACCGCCACGCCTTTGCCACCGCTGTCATGGCTCCTTTTGCTCTCGTTCTCGAGAGGAAAGTAAGACCCAAGATTACATGGAAGATTTTCATTCAGATGTTTGTTCTCGCTCTTCTTGGGCCACTGATCGATCAGAATTTCTACTACGTCGGGCTGAAAATGACATCCCCAACGTACTCGTGTGCCATAAGCAACATGCTTCCTTCGATGACATTCATCATGGCAGTCCTTTGCAGGATGGAGAAGCTGGACTTGAGGAAAGTGAGATGCCAAGCCAAGTTGTTTGGAACAATAGTGACGGTGATGGGAGCCATGTTGATGACATTTTACAAAGGAAGTGTCATCAACTTCTTCTGGACCGAGCAAGCCCACCACCCGAACTCACCACCGACAGCGGATGCGGCTGCGCTCAACCACCACCACGACGGAGAGTTCATCAAAGGCTCAATTTTGCTCATCATAGCCACTCTAGCTTGGGCGGGGTTCTTCATTCTCCAAGTCATAACCTTGAGAAAATACACCGCCCATCTTTCTCTCACAACCATGGTTTGCTTTTTGGGAACCCTTCAGGCCATTGTTGTCACCCTCGCTATGGAACACCGCCCCTCCGCCTGGGCCATCGGCTGGGACATGAACCTTCTCGCCGCCGCTTATGCG

Coding sequence (CDS)

ATGGGATTCATAGAATGCCTTGGCTGTTTCTTTCAAAATGCAAGGCCTTACATCGCAGTCATCTCCCTCCAATTCGGCTACGCCGGGATGAACATCATCTCCGTCGTCTCCCTCAACCGTGGTATGAGCCATTACGTCCTCGTTGTCTACCGCCACGCCTTTGCCACCGCTGTCATGGCTCCTTTTGCTCTCGTTCTCGAGAGGAAAGTAAGACCCAAGATTACATGGAAGATTTTCATTCAGATGTTTGTTCTCGCTCTTCTTGGGCCACTGATCGATCAGAATTTCTACTACGTCGGGCTGAAAATGACATCCCCAACGTACTCGTGTGCCATAAGCAACATGCTTCCTTCGATGACATTCATCATGGCAGTCCTTTGCAGGATGGAGAAGCTGGACTTGAGGAAAGTGAGATGCCAAGCCAAGTTGTTTGGAACAATAGTGACGGTGATGGGAGCCATGTTGATGACATTTTACAAAGGAAGTGTCATCAACTTCTTCTGGACCGAGCAAGCCCACCACCCGAACTCACCACCGACAGCGGATGCGGCTGCGCTCAACCACCACCACGACGGAGAGTTCATCAAAGGCTCAATTTTGCTCATCATAGCCACTCTAGCTTGGGCGGGGTTCTTCATTCTCCAAGTCATAACCTTGAGAAAATACACCGCCCATCTTTCTCTCACAACCATGGTTTGCTTTTTGGGAACCCTTCAGGCCATTGTTGTCACCCTCGCTATGGAACACCGCCCCTCCGCCTGGGCCATCGGCTGGGACATGAACCTTCTCGCCGCCGCTTATGCG

Protein sequence

MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPTADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQAIVVTLAMEHRPSAWAIGWDMNLLAAAYA
Homology
BLAST of Carg01299 vs. NCBI nr
Match: KAG7027706.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 530.4 bits (1365), Expect = 8.9e-147
Identity = 268/268 (100.00%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60
           MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA
Sbjct: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120
           PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120

Query: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180
           FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180

Query: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240
           ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA
Sbjct: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240

Query: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 269
           IVVTLAMEHRPSAWAIGWDMNLLAAAYA
Sbjct: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 268

BLAST of Carg01299 vs. NCBI nr
Match: KAG6596165.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 529.3 bits (1362), Expect = 2.0e-146
Identity = 267/268 (99.63%), Postives = 268/268 (100.00%), Query Frame = 0

Query: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60
           MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA
Sbjct: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120
           PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAI+NMLPSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAITNMLPSMT 120

Query: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180
           FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180

Query: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240
           ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA
Sbjct: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240

Query: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 269
           IVVTLAMEHRPSAWAIGWDMNLLAAAYA
Sbjct: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 268

BLAST of Carg01299 vs. NCBI nr
Match: XP_022933516.1 (WAT1-related protein At5g07050-like [Cucurbita moschata])

HSP 1 Score: 524.2 bits (1349), Expect = 6.4e-145
Identity = 266/268 (99.25%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60
           MGFIECLG FFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA
Sbjct: 1   MGFIECLGRFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120
           PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120

Query: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180
           FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180

Query: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240
           AD AALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA
Sbjct: 181 ADTAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240

Query: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 269
           IVVTLAMEHRPSAWAIGWDMNLLAAAYA
Sbjct: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 268

BLAST of Carg01299 vs. NCBI nr
Match: XP_022971200.1 (WAT1-related protein At5g07050-like [Cucurbita maxima])

HSP 1 Score: 518.1 bits (1333), Expect = 4.6e-143
Identity = 264/268 (98.51%), Postives = 264/268 (98.51%), Query Frame = 0

Query: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60
           MGFIECLG FFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA
Sbjct: 1   MGFIECLGRFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120
           PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120

Query: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180
           FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQA HPNSPPT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQARHPNSPPT 180

Query: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240
           ADA ALNHHHD EFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA
Sbjct: 181 ADAGALNHHHDREFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240

Query: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 269
           IVVTLAMEHRPSAWAIGWDMNLLAAAYA
Sbjct: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 268

BLAST of Carg01299 vs. NCBI nr
Match: XP_023539394.1 (WAT1-related protein At5g07050-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 513.8 bits (1322), Expect = 8.7e-142
Identity = 263/268 (98.13%), Postives = 263/268 (98.13%), Query Frame = 0

Query: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60
           MGFIECL  FFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA
Sbjct: 1   MGFIECLARFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120
           PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120

Query: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180
           FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQA HPNS PT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQARHPNSQPT 180

Query: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240
           ADAAALNHH DGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA
Sbjct: 181 ADAAALNHHDDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240

Query: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 269
           IVVTLAMEHRPSAWAIGWDMNLLAAAYA
Sbjct: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 268

BLAST of Carg01299 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 4.1e-94
Identity = 172/260 (66.15%), Postives = 208/260 (80.00%), Query Frame = 0

Query: 10  FFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERK 69
           F  +++PY A+ISLQFGYAGMNII+ +SLN GMSHYVLVVYRHA ATAV+APFA   ERK
Sbjct: 12  FLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERK 71

Query: 70  VRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRM 129
            +PKIT+ IF+Q+F+L LLGP+IDQNFYY+GLK TSPT+SCA+SNMLP+MTFI+AVL RM
Sbjct: 72  AQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRM 131

Query: 130 EKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPTADAAALNHH 189
           E LDL+K+ CQAK+ GT+VTV GAMLMT YKG ++  FWT+  H  +S      ++ N  
Sbjct: 132 EMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSS 191

Query: 190 HDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAH-LSLTTMVCFLGTLQAIVVTLAME 249
            D EF+KGSILLI ATLAWA  F+LQ   L+ Y  H LSLTT++CF+GTLQA+ VT  ME
Sbjct: 192 SDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVME 251

Query: 250 HRPSAWAIGWDMNLLAAAYA 269
           H PSAW IGWDMNLLAAAY+
Sbjct: 252 HNPSAWRIGWDMNLLAAAYS 271

BLAST of Carg01299 vs. ExPASy Swiss-Prot
Match: F4IJ08 (WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 6.8e-81
Identity = 149/257 (57.98%), Postives = 205/257 (79.77%), Query Frame = 0

Query: 12  QNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERKVR 71
           ++A+PY A++ LQFGYAGMN+++   L+RGMSHYVLV YR+AFATA +APFAL+ ERKVR
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRMEK 131
            K+T+ IF+++F+LALLGP+IDQN YY+GLK+TSPT+S A+SN++P++T I+A L RMEK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 132 LDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPTADAAALNHHHD 191
           +++RKVRC  K+ GT+VTV+G++LM FYKG  INFF        +SPPTAD         
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFF-RSHLTAASSPPTAD--------- 186

Query: 192 GEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQAIVVTLAMEHRP 251
             ++K ++ L++A+L+WA FF+LQ  TL+KY+AHLS++TMVCF+GTLQ++ +   MEH P
Sbjct: 187 --YLKAAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNP 246

Query: 252 SAWAIGWDMNLLAAAYA 269
           SA  IG+DMNLLA+AYA
Sbjct: 247 SALNIGFDMNLLASAYA 251

BLAST of Carg01299 vs. ExPASy Swiss-Prot
Match: Q9LXX8 (WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.1e-78
Identity = 146/257 (56.81%), Postives = 202/257 (78.60%), Query Frame = 0

Query: 12  QNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERKVR 71
           ++A+PY A++ LQFGYAGMN+++ V L+RGMSHYVLV YR+AFATA +APFAL+ ERKVR
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRMEK 131
           PK+T+ IF+Q+FVLALLGPLIDQN YY GLK+TSPT++ A++N++P++TFI++++CRMEK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 132 LDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPTADAAALNHHHD 191
           +++RKVR QAK+ GT+V V+GAMLM  +K  +I F  +    H  SP   D         
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFLRSHLTGHALSPAGED--------- 186

Query: 192 GEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQAIVVTLAMEHRP 251
             ++K ++ L+IA+ +WA FF+LQ  TL++Y++HLSL+TMVCF+GTLQ+  +T  ME   
Sbjct: 187 --YLKATVFLLIASFSWASFFVLQAATLKRYSSHLSLSTMVCFMGTLQSTALTFVMEPNL 246

Query: 252 SAWAIGWDMNLLAAAYA 269
           SAW IG+DMNLLA+AYA
Sbjct: 247 SAWNIGFDMNLLASAYA 252

BLAST of Carg01299 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 3.6e-66
Identity = 131/260 (50.38%), Postives = 187/260 (71.92%), Query Frame = 0

Query: 15  RPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERKVRPKI 74
           +PY+A+IS+QFGYAGM II++VSL  GM+HYVL VYRHA ATAV+APFAL  ERK+RPK+
Sbjct: 10  KPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERKIRPKM 69

Query: 75  TWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRMEKLDL 134
           T++IF+Q+ +L  + P++DQN YYVG+  TS T++ A +N+LP++TF++A++ R+E ++ 
Sbjct: 70  TFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRLESVNF 129

Query: 135 RKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT------ADAAALNH 194
           +KVR  AK+ GT++TV GA+LMT YKG +++F          S         A AAA++ 
Sbjct: 130 KKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGAAAMDK 189

Query: 195 HHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQAIVVTLAME 254
           H    +I G+++L+  T  WAGFFILQ  TL++Y A LSLTT++C +GTL+   V+L   
Sbjct: 190 H----WIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVTV 249

Query: 255 HRPSAWAIGWDMNLLAAAYA 269
              SAW IG+D NL AAAY+
Sbjct: 250 RDLSAWKIGFDSNLFAAAYS 265

BLAST of Carg01299 vs. ExPASy Swiss-Prot
Match: Q501F8 (WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 5.4e-62
Identity = 125/255 (49.02%), Postives = 170/255 (66.67%), Query Frame = 0

Query: 15  RPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERKVRPKI 74
           +P IA+ISLQFGYAGM II++VS   GM+H++L  YRH  AT V+APFAL+LERK+RPK+
Sbjct: 10  KPIIAIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALILERKIRPKM 69

Query: 75  TWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRMEKLDL 134
           TW +F+++  L  L PL+DQN YY+G+K TS TYS A  N LP++TFIMAV+ R+E ++L
Sbjct: 70  TWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVIFRIETVNL 129

Query: 135 RKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAH-HPNSPPTADAAALNHHHDGE 194
           +K R  AK+ GT +TV GAM+MT YKG  I  F T  +  H  S  T+         D  
Sbjct: 130 KKTRSLAKVIGTAITVGGAMVMTLYKGPAIELFKTAHSSLHGGSSGTSSETT-----DQN 189

Query: 195 FIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQAIVVTLAMEHRPSA 254
           ++ G++ ++ +   WAGFFILQ  TL+KY A LSL   +C +GT+   + +L M    SA
Sbjct: 190 WVTGTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSA 249

Query: 255 WAIGWDMNLLAAAYA 269
           W +G D   LAA Y+
Sbjct: 250 WKVGMDSGTLAAVYS 259

BLAST of Carg01299 vs. ExPASy TrEMBL
Match: A0A6J1EZ96 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111440914 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 3.1e-145
Identity = 266/268 (99.25%), Postives = 266/268 (99.25%), Query Frame = 0

Query: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60
           MGFIECLG FFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA
Sbjct: 1   MGFIECLGRFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120
           PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120

Query: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180
           FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180

Query: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240
           AD AALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA
Sbjct: 181 ADTAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240

Query: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 269
           IVVTLAMEHRPSAWAIGWDMNLLAAAYA
Sbjct: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 268

BLAST of Carg01299 vs. ExPASy TrEMBL
Match: A0A6J1I534 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469993 PE=3 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 2.2e-143
Identity = 264/268 (98.51%), Postives = 264/268 (98.51%), Query Frame = 0

Query: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60
           MGFIECLG FFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA
Sbjct: 1   MGFIECLGRFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120
           PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120

Query: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180
           FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQA HPNSPPT
Sbjct: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQARHPNSPPT 180

Query: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240
           ADA ALNHHHD EFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA
Sbjct: 181 ADAGALNHHHDREFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240

Query: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 269
           IVVTLAMEHRPSAWAIGWDMNLLAAAYA
Sbjct: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 268

BLAST of Carg01299 vs. ExPASy TrEMBL
Match: A0A5D3DPD1 (WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006520 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.7e-131
Identity = 242/268 (90.30%), Postives = 255/268 (95.15%), Query Frame = 0

Query: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60
           MGF+EC+G FFQNA PYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA
Sbjct: 1   MGFLECIGRFFQNANPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120
           PFALVLERKVRPKITWKIFIQMF LALLGPLIDQNFYYVGLKMTSPT+SCAISNMLP+MT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFALALLGPLIDQNFYYVGLKMTSPTFSCAISNMLPAMT 120

Query: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180
           F+MAV+CRMEKLDL++VRCQAKLFGTIVTV+GAMLMTFYKGSVIN F T  +H P+S   
Sbjct: 121 FVMAVICRMEKLDLKRVRCQAKLFGTIVTVVGAMLMTFYKGSVINIFGTGHSHQPSS--- 180

Query: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240
           ADAAA+NHHHDGEFIKGSILLIIATLAWA FFILQVITLRKYTAHLSLTTMVCFLGTLQA
Sbjct: 181 ADAAAVNHHHDGEFIKGSILLIIATLAWAAFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240

Query: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 269
           I+VTLAMEHRP AWAIGWDMNLLAAAYA
Sbjct: 241 IIVTLAMEHRPGAWAIGWDMNLLAAAYA 265

BLAST of Carg01299 vs. ExPASy TrEMBL
Match: E5GBP7 (WAT1-related protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.7e-131
Identity = 242/268 (90.30%), Postives = 255/268 (95.15%), Query Frame = 0

Query: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60
           MGF+EC+G FFQNA PYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA
Sbjct: 1   MGFLECIGRFFQNANPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120
           PFALVLERKVRPKITWKIFIQMF LALLGPLIDQNFYYVGLKMTSPT+SCAISNMLP+MT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFALALLGPLIDQNFYYVGLKMTSPTFSCAISNMLPAMT 120

Query: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180
           F+MAV+CRMEKLDL++VRCQAKLFGTIVTV+GAMLMTFYKGSVIN F T  +H P+S   
Sbjct: 121 FVMAVICRMEKLDLKRVRCQAKLFGTIVTVVGAMLMTFYKGSVINIFGTGHSHQPSS--- 180

Query: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240
           ADAAA+NHHHDGEFIKGSILLIIATLAWA FFILQVITLRKYTAHLSLTTMVCFLGTLQA
Sbjct: 181 ADAAAVNHHHDGEFIKGSILLIIATLAWAAFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240

Query: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 269
           I+VTLAMEHRP AWAIGWDMNLLAAAYA
Sbjct: 241 IIVTLAMEHRPGAWAIGWDMNLLAAAYA 265

BLAST of Carg01299 vs. ExPASy TrEMBL
Match: A0A1S3B7I5 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103486873 PE=3 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 5.7e-131
Identity = 242/268 (90.30%), Postives = 255/268 (95.15%), Query Frame = 0

Query: 1   MGFIECLGCFFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60
           MGF+EC+G FFQNA PYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA
Sbjct: 1   MGFLECIGRFFQNANPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMA 60

Query: 61  PFALVLERKVRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMT 120
           PFALVLERKVRPKITWKIFIQMF LALLGPLIDQNFYYVGLKMTSPT+SCAISNMLP+MT
Sbjct: 61  PFALVLERKVRPKITWKIFIQMFALALLGPLIDQNFYYVGLKMTSPTFSCAISNMLPAMT 120

Query: 121 FIMAVLCRMEKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT 180
           F+MAV+CRMEKLDL++VRCQAKLFGTIVTV+GAMLMTFYKGSVIN F T  +H P+S   
Sbjct: 121 FVMAVICRMEKLDLKRVRCQAKLFGTIVTVVGAMLMTFYKGSVINIFGTGHSHQPSS--- 180

Query: 181 ADAAALNHHHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240
           ADAAA+NHHHDGEFIKGSILLIIATLAWA FFILQVITLRKYTAHLSLTTMVCFLGTLQA
Sbjct: 181 ADAAAVNHHHDGEFIKGSILLIIATLAWAAFFILQVITLRKYTAHLSLTTMVCFLGTLQA 240

Query: 241 IVVTLAMEHRPSAWAIGWDMNLLAAAYA 269
           I+VTLAMEHRP AWAIGWDMNLLAAAYA
Sbjct: 241 IIVTLAMEHRPGAWAIGWDMNLLAAAYA 265

BLAST of Carg01299 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 345.9 bits (886), Expect = 2.9e-95
Identity = 172/260 (66.15%), Postives = 208/260 (80.00%), Query Frame = 0

Query: 10  FFQNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERK 69
           F  +++PY A+ISLQFGYAGMNII+ +SLN GMSHYVLVVYRHA ATAV+APFA   ERK
Sbjct: 12  FLTSSKPYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERK 71

Query: 70  VRPKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRM 129
            +PKIT+ IF+Q+F+L LLGP+IDQNFYY+GLK TSPT+SCA+SNMLP+MTFI+AVL RM
Sbjct: 72  AQPKITFSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRM 131

Query: 130 EKLDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPTADAAALNHH 189
           E LDL+K+ CQAK+ GT+VTV GAMLMT YKG ++  FWT+  H  +S      ++ N  
Sbjct: 132 EMLDLKKLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSS 191

Query: 190 HDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAH-LSLTTMVCFLGTLQAIVVTLAME 249
            D EF+KGSILLI ATLAWA  F+LQ   L+ Y  H LSLTT++CF+GTLQA+ VT  ME
Sbjct: 192 SDKEFLKGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVME 251

Query: 250 HRPSAWAIGWDMNLLAAAYA 269
           H PSAW IGWDMNLLAAAY+
Sbjct: 252 HNPSAWRIGWDMNLLAAAYS 271

BLAST of Carg01299 vs. TAIR 10
Match: AT2G40900.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 302.0 bits (772), Expect = 4.8e-82
Identity = 149/257 (57.98%), Postives = 205/257 (79.77%), Query Frame = 0

Query: 12  QNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERKVR 71
           ++A+PY A++ LQFGYAGMN+++   L+RGMSHYVLV YR+AFATA +APFAL+ ERKVR
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKTVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRMEK 131
            K+T+ IF+++F+LALLGP+IDQN YY+GLK+TSPT+S A+SN++P++T I+A L RMEK
Sbjct: 67  SKMTFPIFMRIFLLALLGPVIDQNLYYIGLKLTSPTFSSAVSNIVPAITIILATLFRMEK 126

Query: 132 LDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPTADAAALNHHHD 191
           +++RKVRC  K+ GT+VTV+G++LM FYKG  INFF        +SPPTAD         
Sbjct: 127 VEMRKVRCLVKVMGTLVTVVGSILMIFYKGPFINFF-RSHLTAASSPPTAD--------- 186

Query: 192 GEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQAIVVTLAMEHRP 251
             ++K ++ L++A+L+WA FF+LQ  TL+KY+AHLS++TMVCF+GTLQ++ +   MEH P
Sbjct: 187 --YLKAAVFLLLASLSWASFFVLQAATLKKYSAHLSMSTMVCFMGTLQSLALAFVMEHNP 246

Query: 252 SAWAIGWDMNLLAAAYA 269
           SA  IG+DMNLLA+AYA
Sbjct: 247 SALNIGFDMNLLASAYA 251

BLAST of Carg01299 vs. TAIR 10
Match: AT3G56620.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 294.7 bits (753), Expect = 7.7e-80
Identity = 146/257 (56.81%), Postives = 202/257 (78.60%), Query Frame = 0

Query: 12  QNARPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERKVR 71
           ++A+PY A++ LQFGYAGMN+++ V L+RGMSHYVLV YR+AFATA +APFAL+ ERKVR
Sbjct: 7   ESAKPYFAMVCLQFGYAGMNLVTKVVLDRGMSHYVLVAYRNAFATAAIAPFALLSERKVR 66

Query: 72  PKITWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRMEK 131
           PK+T+ IF+Q+FVLALLGPLIDQN YY GLK+TSPT++ A++N++P++TFI++++CRMEK
Sbjct: 67  PKMTFPIFMQIFVLALLGPLIDQNLYYAGLKLTSPTFAGAVTNIVPALTFIISIICRMEK 126

Query: 132 LDLRKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPTADAAALNHHHD 191
           +++RKVR QAK+ GT+V V+GAMLM  +K  +I F  +    H  SP   D         
Sbjct: 127 VEMRKVRFQAKVVGTLVIVVGAMLMILFKIPLITFLRSHLTGHALSPAGED--------- 186

Query: 192 GEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQAIVVTLAMEHRP 251
             ++K ++ L+IA+ +WA FF+LQ  TL++Y++HLSL+TMVCF+GTLQ+  +T  ME   
Sbjct: 187 --YLKATVFLLIASFSWASFFVLQAATLKRYSSHLSLSTMVCFMGTLQSTALTFVMEPNL 246

Query: 252 SAWAIGWDMNLLAAAYA 269
           SAW IG+DMNLLA+AYA
Sbjct: 247 SAWNIGFDMNLLASAYA 252

BLAST of Carg01299 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 253.1 bits (645), Expect = 2.6e-67
Identity = 131/260 (50.38%), Postives = 187/260 (71.92%), Query Frame = 0

Query: 15  RPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERKVRPKI 74
           +PY+A+IS+QFGYAGM II++VSL  GM+HYVL VYRHA ATAV+APFAL  ERK+RPK+
Sbjct: 10  KPYLAMISMQFGYAGMYIITMVSLKHGMNHYVLAVYRHAIATAVIAPFALFHERKIRPKM 69

Query: 75  TWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRMEKLDL 134
           T++IF+Q+ +L  + P++DQN YYVG+  TS T++ A +N+LP++TF++A++ R+E ++ 
Sbjct: 70  TFRIFLQIALLGFIEPVLDQNLYYVGMTYTSATFASATANVLPAITFVLAIIFRLESVNF 129

Query: 135 RKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAHHPNSPPT------ADAAALNH 194
           +KVR  AK+ GT++TV GA+LMT YKG +++F          S         A AAA++ 
Sbjct: 130 KKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGGGGGGSDGAGGSHGGAGAAAMDK 189

Query: 195 HHDGEFIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQAIVVTLAME 254
           H    +I G+++L+  T  WAGFFILQ  TL++Y A LSLTT++C +GTL+   V+L   
Sbjct: 190 H----WIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTLEGTAVSLVTV 249

Query: 255 HRPSAWAIGWDMNLLAAAYA 269
              SAW IG+D NL AAAY+
Sbjct: 250 RDLSAWKIGFDSNLFAAAYS 265

BLAST of Carg01299 vs. TAIR 10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 239.2 bits (609), Expect = 3.8e-63
Identity = 125/255 (49.02%), Postives = 170/255 (66.67%), Query Frame = 0

Query: 15  RPYIAVISLQFGYAGMNIISVVSLNRGMSHYVLVVYRHAFATAVMAPFALVLERKVRPKI 74
           +P IA+ISLQFGYAGM II++VS   GM+H++L  YRH  AT V+APFAL+LERK+RPK+
Sbjct: 10  KPIIAIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALILERKIRPKM 69

Query: 75  TWKIFIQMFVLALLGPLIDQNFYYVGLKMTSPTYSCAISNMLPSMTFIMAVLCRMEKLDL 134
           TW +F+++  L  L PL+DQN YY+G+K TS TYS A  N LP++TFIMAV+ R+E ++L
Sbjct: 70  TWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVIFRIETVNL 129

Query: 135 RKVRCQAKLFGTIVTVMGAMLMTFYKGSVINFFWTEQAH-HPNSPPTADAAALNHHHDGE 194
           +K R  AK+ GT +TV GAM+MT YKG  I  F T  +  H  S  T+         D  
Sbjct: 130 KKTRSLAKVIGTAITVGGAMVMTLYKGPAIELFKTAHSSLHGGSSGTSSETT-----DQN 189

Query: 195 FIKGSILLIIATLAWAGFFILQVITLRKYTAHLSLTTMVCFLGTLQAIVVTLAMEHRPSA 254
           ++ G++ ++ +   WAGFFILQ  TL+KY A LSL   +C +GT+   + +L M    SA
Sbjct: 190 WVTGTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMVRDVSA 249

Query: 255 WAIGWDMNLLAAAYA 269
           W +G D   LAA Y+
Sbjct: 250 WKVGMDSGTLAAVYS 259

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027706.18.9e-147100.00WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6596165.12.0e-14699.63WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022933516.16.4e-14599.25WAT1-related protein At5g07050-like [Cucurbita moschata][more]
XP_022971200.14.6e-14398.51WAT1-related protein At5g07050-like [Cucurbita maxima][more]
XP_023539394.18.7e-14298.13WAT1-related protein At5g07050-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9FL414.1e-9466.15WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
F4IJ086.8e-8157.98WAT1-related protein At2g40900 OS=Arabidopsis thaliana OX=3702 GN=At2g40900 PE=2... [more]
Q9LXX81.1e-7856.81WAT1-related protein At3g56620 OS=Arabidopsis thaliana OX=3702 GN=At3g56620 PE=2... [more]
F4HZQ73.6e-6650.38WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2... [more]
Q501F85.4e-6249.02WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1EZ963.1e-14599.25WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111440914 PE=3 SV=1[more]
A0A6J1I5342.2e-14398.51WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469993 PE=3 SV=1[more]
A0A5D3DPD15.7e-13190.30WAT1-related protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352... [more]
E5GBP75.7e-13190.30WAT1-related protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A1S3B7I55.7e-13190.30WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103486873 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G07050.12.9e-9566.15nodulin MtN21 /EamA-like transporter family protein [more]
AT2G40900.14.8e-8257.98nodulin MtN21 /EamA-like transporter family protein [more]
AT3G56620.17.7e-8056.81nodulin MtN21 /EamA-like transporter family protein [more]
AT1G21890.12.6e-6750.38nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08300.13.8e-6349.02nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 17..157
e-value: 3.1E-11
score: 43.6
NoneNo IPR availablePANTHERPTHR31218:SF342WAT1-RELATED PROTEINcoord: 11..268
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 50..159
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 11..268

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg01299-RACarg01299-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity