Carg00525 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg00525
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionATP-dependent 6-phosphofructokinase
LocationCarg_Chr03: 5432382 .. 5434839 (-)
RNA-Seq ExpressionCarg00525
SyntenyCarg00525
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACCGGTTCATTCTTCATTTCACTCATCCCCCCAAACTCGCCTCTCAATTTCCCCTTCCCCTAAGATGGCCACCACCGCCGCCGCCGAAATCGCTGACCGGCACCCCCCCATCGCCGTCGCAAGCCTGCCCCACCTGACCGACCACTTCCCGACTCTCCAAAGCAGAACCAACCCACTCCAGAACAACCAATTCTTCCACCCAATAGACGGCTTCTACGCCACCTCCACCGACGTGGTCCTTCGCCGCATCGCCTTCGACCTCTCCAGAAAGCCTTCTCCAGGACACGTGGCGTACTACCGAGCTGGTCCACGGCGGTCGGTTTACTTCGAACCGGAGTCGGTTCGAGCGGCGATCGTGACGTGTGGAGGGCTGTGCCCCGGGATGAACACGGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAGCTGTACGGAGTGCGGCAGATACATGGGATTGTGGCTGGCTACAGAGGGTTCTACTCGAGCGAGCCGGTGGAGCTGAGTCTGAAGCTGGTCGATGATTGGCATAAGAGAGGCGGCACCGCCCTTCGAACCTCCCGAGGTGGGTTTGATCTCCGGAGGATCGTCGATGCCATCCAAGATCGTGGTTTCAATCAGGTTTTTTTAATTTAATTCTAAATTTAAATCATTATTTATTTTAATTTAGAGTTAGTTTGAAGGCATTTAAAAAAATGGGACACTTGGCGCGCCAACACTGAAACTTAGGATGTTTTTTTGGTAGTTAATAATGTGTCGTGGGGCGGGAGATGTCCACGTGTCTAATTTGTAATTTTTATTCTTTATGATTATTATTATTATTATTTTAAAGTTTTAATATTTCAATATAATCTTTATAAAAAAAAAAAAAAAAAAATCCTAATAAATAAAATATATTTATTATGCAAACATCAGCCTAACATAATTTTAAAAAATATTACTAGAAATATAAAATGTAATATTTACAAACATAGAAAAAAACCCAAAGCGTATATATATATATATATATATTATCTGTGTGTGTGTGTGTATATGTGTAGATATATATATATGTGTGTGTGTAATGTATATATATATGTATGTGTGTGTATGCGTATATGTGTGTATAGATATATATGTGTGTGTATATATATGTGTATGTATGTGTATATGTGTGTATAGATATATATGTGTGTGTGTGTATATATATGTATGTATGCTGTCTGTCTATATATATGTATGTATGTATGTATGTATGTATGTATATCTGTCTAATATATATATATCTATATGTATGTATGTATATGTATGTATGTCTCTATATATATGTATGTATGTCTGTCTAATATATATATATATATATATATATCTATATCTATGTATGTATGTATGTCTATGTATGTCTATGTATGTATCTATATATATATTTTTATATATATCCAGGTTTGTCGGGCTAGCAGATAAAATGGATATCTCTAATGGCTACGCTAAATAATTTCTTTAATATTTCTGTTTCCTAATTTCTCCAATAGTTTTCATTGTCAAGAATTTTCATATTTGGATTAGGAATTAGGATTGTTTTAATGGGAGATTTCATTGCAAATTAATTCACTAGGTCTATATAATCGGAGGAGATGGCACAATGCGCGGCGCCGTCAAGATATTCGAAGAAGTATGTCGTCGGAATCTGCAAGTCGCCGTCACCGGGATCCCGAAAACAGTTGACAATGACATAGGAATCATCGACCGATCATTCGGATTCCAAACAGCCGTCGAAATGGCGCAGCAGGCAATCAGCGCCGCTCATGTGGAGGCTGAGAGCGCAGTGAACGGCATAGGTCTCGTGAAATTGATGGGCAGAAGCACTGGCCATATCGCTCTTCACGCCACATTGAGCAGTCGAGACGTCGATTGCTGCTTAATACCTGAAATGGAGTTTTATTTGGAAGGCGACGGAGGTTTGTTTCCGTTTCTTGAGCGGAGGCTGAAGGAGAATGGGCATGCGGTTCTGGTGGTTGCAGAGGGAGCAGGACAGGATACAATACCTAGAACTGAGGCACAAAAGGAAGAGCGGGACGAATCTGGAAATCCGGTGTTCTTGGATGTGGGCGGATGGTTAAAGACGGAACTCAAAGATTGGTGGGCGAGAACGCACCCCGGTGAGTTGTTTACTGTTAAATACATAGATCCTACTTACATGATTCGTGCTGTGCCTGCAAATGCTACAGATAACTCCTATTGTACATTGCTTGCTCATTCTGCAATTCATGGGGTAATGGCTGGTTATACTGGATTTGTCTCTGGCCCTATTAATGGAAACTATTCGTATATTCCTCTGAAAGATGTAGCAGAAGCAAAGAATGAGGTTAATACGAGAGATCATAAGTGGGCATGGGTTAGATCTGTCACTAATCAGCCTGATTTTGTAAAAACTCGACTATTCGATCCAGAAAATCAGAGTTCATCAGGGATTTCTAGCTGA

mRNA sequence

CAACCGGTTCATTCTTCATTTCACTCATCCCCCCAAACTCGCCTCTCAATTTCCCCTTCCCCTAAGATGGCCACCACCGCCGCCGCCGAAATCGCTGACCGGCACCCCCCCATCGCCGTCGCAAGCCTGCCCCACCTGACCGACCACTTCCCGACTCTCCAAAGCAGAACCAACCCACTCCAGAACAACCAATTCTTCCACCCAATAGACGGCTTCTACGCCACCTCCACCGACGTGGTCCTTCGCCGCATCGCCTTCGACCTCTCCAGAAAGCCTTCTCCAGGACACGTGGCGTACTACCGAGCTGGTCCACGGCGGTCGGTTTACTTCGAACCGGAGTCGGTTCGAGCGGCGATCGTGACGTGTGGAGGGCTGTGCCCCGGGATGAACACGGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAGCTGTACGGAGTGCGGCAGATACATGGGATTGTGGCTGGCTACAGAGGGTTCTACTCGAGCGAGCCGGTGGAGCTGAGTCTGAAGCTGGTCGATGATTGGCATAAGAGAGGCGGCACCGCCCTTCGAACCTCCCGAGGTGGGTTTGATCTCCGGAGGATCGTCGATGCCATCCAAGATCGTGGTTTCAATCAGGTCTATATAATCGGAGGAGATGGCACAATGCGCGGCGCCGTCAAGATATTCGAAGAAGTATGTCGTCGGAATCTGCAAGTCGCCGTCACCGGGATCCCGAAAACAGTTGACAATGACATAGGAATCATCGACCGATCATTCGGATTCCAAACAGCCGTCGAAATGGCGCAGCAGGCAATCAGCGCCGCTCATGTGGAGGCTGAGAGCGCAGTGAACGGCATAGGTCTCGTGAAATTGATGGGCAGAAGCACTGGCCATATCGCTCTTCACGCCACATTGAGCAGTCGAGACGTCGATTGCTGCTTAATACCTGAAATGGAGTTTTATTTGGAAGGCGACGGAGGTTTGTTTCCGTTTCTTGAGCGGAGGCTGAAGGAGAATGGGCATGCGGTTCTGGTGGTTGCAGAGGGAGCAGGACAGGATACAATACCTAGAACTGAGGCACAAAAGGAAGAGCGGGACGAATCTGGAAATCCGGTGTTCTTGGATGTGGGCGGATGGTTAAAGACGGAACTCAAAGATTGGTGGGCGAGAACGCACCCCGGTGAGTTGTTTACTGTTAAATACATAGATCCTACTTACATGATTCGTGCTGTGCCTGCAAATGCTACAGATAACTCCTATTGTACATTGCTTGCTCATTCTGCAATTCATGGGGTAATGGCTGGTTATACTGGATTTGTCTCTGGCCCTATTAATGGAAACTATTCGTATATTCCTCTGAAAGATGTAGCAGAAGCAAAGAATGAGGTTAATACGAGAGATCATAAGTGGGCATGGGTTAGATCTGTCACTAATCAGCCTGATTTTGTAAAAACTCGACTATTCGATCCAGAAAATCAGAGTTCATCAGGGATTTCTAGCTGA

Coding sequence (CDS)

ATGGCCACCACCGCCGCCGCCGAAATCGCTGACCGGCACCCCCCCATCGCCGTCGCAAGCCTGCCCCACCTGACCGACCACTTCCCGACTCTCCAAAGCAGAACCAACCCACTCCAGAACAACCAATTCTTCCACCCAATAGACGGCTTCTACGCCACCTCCACCGACGTGGTCCTTCGCCGCATCGCCTTCGACCTCTCCAGAAAGCCTTCTCCAGGACACGTGGCGTACTACCGAGCTGGTCCACGGCGGTCGGTTTACTTCGAACCGGAGTCGGTTCGAGCGGCGATCGTGACGTGTGGAGGGCTGTGCCCCGGGATGAACACGGTGATCAGAGAGCTGGTGGTGGGGCTGTGGGAGCTGTACGGAGTGCGGCAGATACATGGGATTGTGGCTGGCTACAGAGGGTTCTACTCGAGCGAGCCGGTGGAGCTGAGTCTGAAGCTGGTCGATGATTGGCATAAGAGAGGCGGCACCGCCCTTCGAACCTCCCGAGGTGGGTTTGATCTCCGGAGGATCGTCGATGCCATCCAAGATCGTGGTTTCAATCAGGTCTATATAATCGGAGGAGATGGCACAATGCGCGGCGCCGTCAAGATATTCGAAGAAGTATGTCGTCGGAATCTGCAAGTCGCCGTCACCGGGATCCCGAAAACAGTTGACAATGACATAGGAATCATCGACCGATCATTCGGATTCCAAACAGCCGTCGAAATGGCGCAGCAGGCAATCAGCGCCGCTCATGTGGAGGCTGAGAGCGCAGTGAACGGCATAGGTCTCGTGAAATTGATGGGCAGAAGCACTGGCCATATCGCTCTTCACGCCACATTGAGCAGTCGAGACGTCGATTGCTGCTTAATACCTGAAATGGAGTTTTATTTGGAAGGCGACGGAGGTTTGTTTCCGTTTCTTGAGCGGAGGCTGAAGGAGAATGGGCATGCGGTTCTGGTGGTTGCAGAGGGAGCAGGACAGGATACAATACCTAGAACTGAGGCACAAAAGGAAGAGCGGGACGAATCTGGAAATCCGGTGTTCTTGGATGTGGGCGGATGGTTAAAGACGGAACTCAAAGATTGGTGGGCGAGAACGCACCCCGGTGAGTTGTTTACTGTTAAATACATAGATCCTACTTACATGATTCGTGCTGTGCCTGCAAATGCTACAGATAACTCCTATTGTACATTGCTTGCTCATTCTGCAATTCATGGGGTAATGGCTGGTTATACTGGATTTGTCTCTGGCCCTATTAATGGAAACTATTCGTATATTCCTCTGAAAGATGTAGCAGAAGCAAAGAATGAGGTTAATACGAGAGATCATAAGTGGGCATGGGTTAGATCTGTCACTAATCAGCCTGATTTTGTAAAAACTCGACTATTCGATCCAGAAAATCAGAGTTCATCAGGGATTTCTAGCTGA

Protein sequence

MATTAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKTRLFDPENQSSSGISS
Homology
BLAST of Carg00525 vs. NCBI nr
Match: KAG6603845.1 (ATP-dependent 6-phosphofructokinase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034027.1 ATP-dependent 6-phosphofructokinase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 970.7 bits (2508), Expect = 4.6e-279
Identity = 472/472 (100.00%), Postives = 472/472 (100.00%), Query Frame = 0

Query: 1   MATTAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLR 60
           MATTAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLR
Sbjct: 1   MATTAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLR 60

Query: 61  RIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWE 120
           RIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWE
Sbjct: 61  RIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWE 120

Query: 121 LYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDR 180
           LYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDR
Sbjct: 121 LYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDR 180

Query: 181 GFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMA 240
           GFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMA
Sbjct: 181 GFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMA 240

Query: 241 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL 300
           QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL
Sbjct: 241 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL 300

Query: 301 FPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWW 360
           FPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWW
Sbjct: 301 FPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWW 360

Query: 361 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNY 420
           ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNY
Sbjct: 361 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNY 420

Query: 421 SYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKTRLFDPENQSSSGISS 473
           SYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKTRLFDPENQSSSGISS
Sbjct: 421 SYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKTRLFDPENQSSSGISS 472

BLAST of Carg00525 vs. NCBI nr
Match: XP_023544772.1 (ATP-dependent 6-phosphofructokinase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 965.7 bits (2495), Expect = 1.5e-277
Identity = 469/472 (99.36%), Postives = 470/472 (99.58%), Query Frame = 0

Query: 1   MATTAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLR 60
           MAT AAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLR
Sbjct: 1   MATAAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLR 60

Query: 61  RIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWE 120
           RIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWE
Sbjct: 61  RIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWE 120

Query: 121 LYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDR 180
           LYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDR
Sbjct: 121 LYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDR 180

Query: 181 GFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMA 240
           GFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMA
Sbjct: 181 GFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMA 240

Query: 241 QQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL 300
           QQA+SAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL
Sbjct: 241 QQAVSAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGL 300

Query: 301 FPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWW 360
           FPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWW
Sbjct: 301 FPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWW 360

Query: 361 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNY 420
           ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNY
Sbjct: 361 ARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNY 420

Query: 421 SYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKTRLFDPENQSSSGISS 473
           SYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKT LFDPENQSSSGISS
Sbjct: 421 SYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKTPLFDPENQSSSGISS 472

BLAST of Carg00525 vs. NCBI nr
Match: XP_022977934.1 (ATP-dependent 6-phosphofructokinase 2 [Cucurbita maxima])

HSP 1 Score: 954.9 bits (2467), Expect = 2.6e-274
Identity = 466/473 (98.52%), Postives = 469/473 (99.15%), Query Frame = 0

Query: 1   MAT-TAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVL 60
           MAT TAAAEI+DRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVL
Sbjct: 1   MATATAAAEISDRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVL 60

Query: 61  RRIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLW 120
           RRIAFDLSRKPSP HVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLW
Sbjct: 61  RRIAFDLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLW 120

Query: 121 ELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQD 180
           ELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQD
Sbjct: 121 ELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQD 180

Query: 181 RGFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEM 240
           RGFNQVYIIGGDGTMRGAVKIFEEVCRR LQVAVTGIPKTVDNDIGIIDRSFGFQTAVEM
Sbjct: 181 RGFNQVYIIGGDGTMRGAVKIFEEVCRRELQVAVTGIPKTVDNDIGIIDRSFGFQTAVEM 240

Query: 241 AQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG 300
           AQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG
Sbjct: 241 AQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG 300

Query: 301 LFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDW 360
           LFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDW
Sbjct: 301 LFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDW 360

Query: 361 WARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGN 420
           WARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGN
Sbjct: 361 WARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGN 420

Query: 421 YSYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKTRLFDPENQSSSGISS 473
           YSYIPLKDVAEAKNEVNT+DHKWAWVRSVTNQPDFV+TRLFDP NQSSSGISS
Sbjct: 421 YSYIPLKDVAEAKNEVNTKDHKWAWVRSVTNQPDFVETRLFDPGNQSSSGISS 473

BLAST of Carg00525 vs. NCBI nr
Match: XP_022950529.1 (ATP-dependent 6-phosphofructokinase 2 [Cucurbita moschata])

HSP 1 Score: 954.1 bits (2465), Expect = 4.4e-274
Identity = 464/469 (98.93%), Postives = 466/469 (99.36%), Query Frame = 0

Query: 4   TAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIA 63
           TAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIA
Sbjct: 3   TAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIA 62

Query: 64  FDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 123
           FDLSRKPSP HVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG
Sbjct: 63  FDLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 122

Query: 124 VRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFN 183
           VRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFN
Sbjct: 123 VRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFN 182

Query: 184 QVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 243
           QVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA
Sbjct: 183 QVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 242

Query: 244 ISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPF 303
           ISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPF
Sbjct: 243 ISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPF 302

Query: 304 LERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWART 363
           LERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWART
Sbjct: 303 LERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWART 362

Query: 364 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 423
           HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI
Sbjct: 363 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 422

Query: 424 PLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKTRLFDPENQSSSGISS 473
           PLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFV+TRLF P N+SSSGISS
Sbjct: 423 PLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVETRLFVPGNRSSSGISS 471

BLAST of Carg00525 vs. NCBI nr
Match: XP_038881531.1 (ATP-dependent 6-phosphofructokinase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 869.8 bits (2246), Expect = 1.1e-248
Identity = 416/453 (91.83%), Postives = 438/453 (96.69%), Query Frame = 0

Query: 5   AAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAF 64
           A A+I+D+HPPIAVA LPHLTD FP L++RTNPLQ NQFFHPIDGFYA+STDVVLRRIAF
Sbjct: 2   ATAKISDQHPPIAVADLPHLTDDFPDLETRTNPLQTNQFFHPIDGFYASSTDVVLRRIAF 61

Query: 65  DLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 124
           DLS K SP HVAY+RAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV
Sbjct: 62  DLSTKESPAHVAYHRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 121

Query: 125 RQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQ 184
           R+I+GI+AGYRGFYS+EPVEL+LKLVD+WHKRGGTALRTSRGGFDLR+IVD IQ  GFNQ
Sbjct: 122 RRIYGILAGYRGFYSTEPVELNLKLVDNWHKRGGTALRTSRGGFDLRKIVDTIQHHGFNQ 181

Query: 185 VYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 244
           VYIIGGDGTMRGAVKIF+E+ RRNLQVAVTGIPKTVDNDIGI+DRSFGFQTAVEMAQQAI
Sbjct: 182 VYIIGGDGTMRGAVKIFDEIRRRNLQVAVTGIPKTVDNDIGIVDRSFGFQTAVEMAQQAI 241

Query: 245 SAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFL 304
           SAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEGDGGLFPFL
Sbjct: 242 SAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGDGGLFPFL 301

Query: 305 ERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTH 364
           ERRLKENGHAVLVVAEGAGQ  IPRTEA+KEERDESGNPVFLDVGGWLKTELK+WWARTH
Sbjct: 302 ERRLKENGHAVLVVAEGAGQSMIPRTEAEKEERDESGNPVFLDVGGWLKTELKNWWARTH 361

Query: 365 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 424
           PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP
Sbjct: 362 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 421

Query: 425 LKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKT 458
           LK+VAEAKNEVNTRDHKWAWVRSVTNQPDFV++
Sbjct: 422 LKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVRS 454

BLAST of Carg00525 vs. ExPASy Swiss-Prot
Match: Q9FIK0 (ATP-dependent 6-phosphofructokinase 2 OS=Arabidopsis thaliana OX=3702 GN=PFK2 PE=1 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.2e-200
Identity = 335/438 (76.48%), Postives = 379/438 (86.53%), Query Frame = 0

Query: 17  AVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVA 76
           ++  LP L+     L+ R NPL++N +FHP +GFY T +DV+L ++A+D S   S   VA
Sbjct: 6   SIRKLPSLSG----LRHRRNPLEDNPYFHPSNGFYITPSDVILAQVAYDHSAH-SQSRVA 65

Query: 77  YYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRG 136
           Y+RAGPRR + +EP +V+AAIVTCGGLCPGMNTVIRELVVGLWELYGVR+I+GI AGYRG
Sbjct: 66  YHRAGPRREIMYEPSAVKAAIVTCGGLCPGMNTVIRELVVGLWELYGVREIYGIPAGYRG 125

Query: 137 FYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRG 196
           FYS + V+L  K V DWHK+GGT L TSRGGF L++IVDAI   G+NQVYIIGGDGTMRG
Sbjct: 126 FYSMKAVKLDPKAVHDWHKKGGTVLATSRGGFHLQKIVDAIHLNGYNQVYIIGGDGTMRG 185

Query: 197 AVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVN 256
           AV+IF+E+  R L+V +T IPKTVDND+GIIDRSFGFQTAVEMAQ+AISAAHVEAESAVN
Sbjct: 186 AVEIFKEISLRKLEVGITVIPKTVDNDVGIIDRSFGFQTAVEMAQEAISAAHVEAESAVN 245

Query: 257 GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVL 316
           GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM+FYLEG GGLF FLE+RLKE GHAVL
Sbjct: 246 GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMDFYLEGKGGLFEFLEKRLKERGHAVL 305

Query: 317 VVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDP 376
           VVAEGAGQ+ IPR E+QK+ERDESGN VFLDVG W K+ LK WW R HP ELFTVKYIDP
Sbjct: 306 VVAEGAGQEMIPRNESQKQERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTVKYIDP 365

Query: 377 TYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVN 436
           TYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGNY+YIPL++VA+ KN+VN
Sbjct: 366 TYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQTKNQVN 425

Query: 437 TRDHKWAWVRSVTNQPDF 455
           TRDHKWAWVRSVTNQPDF
Sbjct: 426 TRDHKWAWVRSVTNQPDF 438

BLAST of Carg00525 vs. ExPASy Swiss-Prot
Match: Q94AA4 (ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE=1 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 4.8e-146
Identity = 257/451 (56.98%), Postives = 327/451 (72.51%), Query Frame = 0

Query: 21  LPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVAYYRA 80
           +PHL+D+ P L +  NPLQ+N  +  +  ++  + D V ++I   +  K  P  + + RA
Sbjct: 23  VPHLSDYLPGLPTYPNPLQDNPAYSVVKQYFVDADDSVPQKI---VVHKDGPRGIHFRRA 82

Query: 81  GPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRGFYSS 140
           GPR+ VYFE + V A IVTCGGLCPG+NTVIRE+V  L  +YGV++I GI  GYRGFY+ 
Sbjct: 83  GPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFYAK 142

Query: 141 EPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRGAVKI 200
             V L  K+V+D HKRGGT L TSRGG D  +IVD+IQDRG NQVYIIGGDGT RGA  I
Sbjct: 143 NTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRGASVI 202

Query: 201 FEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVNGIGL 260
           FEE+ RR L+VAV GIPKT+DNDI +ID+SFGF TAVE AQ+AI+AAHVEAES  NGIG+
Sbjct: 203 FEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIENGIGV 262

Query: 261 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVLVVAE 320
           VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG+GGLF ++E+RLKE+GH VLV+AE
Sbjct: 263 VKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVLVIAE 322

Query: 321 GAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDPTYMI 380
           GAGQD + ++      +D SGN +  DVG WL   +KD +       +  +KYIDPTYMI
Sbjct: 323 GAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHF--NQKKMVMNLKYIDPTYMI 382

Query: 381 RAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVNTRDH 440
           RAVP+NA+DN YCTLLA SA+HG MAGYTG++SG +NG  +YIP   + E +N V   D 
Sbjct: 383 RAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVITDR 442

Query: 441 KWAWVRSVTNQPDFVKTR-LFDPENQSSSGI 471
            WA + S TNQP F+  + +FD + +  S +
Sbjct: 443 MWARLLSSTNQPSFLGPKDVFDNKEKPMSAL 468

BLAST of Carg00525 vs. ExPASy Swiss-Prot
Match: Q9FKG3 (ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PFK4 PE=1 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 5.3e-145
Identity = 253/435 (58.16%), Postives = 315/435 (72.41%), Query Frame = 0

Query: 21  LPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVAYYRA 80
           +PHLT   P L S  NPL+ +Q +  +   + +S DVV + I   + +K S   V + RA
Sbjct: 74  VPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNI---VVQKGSKRGVHFRRA 133

Query: 81  GPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRGFYSS 140
           GPR  VYF  + V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  GYRGFYS 
Sbjct: 134 GPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGYRGFYSK 193

Query: 141 EPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRGAVKI 200
             + L+ K+V+D HKRGGT L+TSRGG D  +IVD IQDRG NQVYIIGG GT +GA KI
Sbjct: 194 NTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAEKI 253

Query: 201 FEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVNGIGL 260
           +EEV RR LQVAV+GIPKT+DNDI +ID+SFGF TAVE AQ+AI+AAHVE ES  NG+G+
Sbjct: 254 YEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVENGVGI 313

Query: 261 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVLVVAE 320
           VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F+E RLKEN H V+V+AE
Sbjct: 314 VKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHMVIVIAE 373

Query: 321 GAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDPTYMI 380
           GAGQD + ++    E +D SGN + LDVG WL  ++KD +       +  +KYIDPTYMI
Sbjct: 374 GAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKYIDPTYMI 433

Query: 381 RAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVNTRDH 440
           RA+P+NA+DN YCTLLA SA+HG MAGY+GF  GP+N  ++YIP+  V E  N V   D 
Sbjct: 434 RAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTVKLTDR 493

Query: 441 KWAWVRSVTNQPDFV 456
            WA + + TNQP F+
Sbjct: 494 MWARLLASTNQPSFL 504

BLAST of Carg00525 vs. ExPASy Swiss-Prot
Match: Q9C5J7 (ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 3.4e-144
Identity = 252/435 (57.93%), Postives = 318/435 (73.10%), Query Frame = 0

Query: 21  LPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVAYYRA 80
           +PHL D+ P L +  NPLQ+N  +  +  ++  + D V  ++   +  K  P  V + RA
Sbjct: 23  VPHLIDYLPDLPTYPNPLQDNPAYSVVKQYFVHADDSVPEKV---VVHKDGPRGVHFRRA 82

Query: 81  GPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRGFYSS 140
           GPR+ VYFE + V A IVTCGGLCPG+NTVIRE+V  L  +YGV++I GI  GYRGFY+ 
Sbjct: 83  GPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFYAK 142

Query: 141 EPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRGAVKI 200
             + L+ K+V+D HKRGGT + TSRGG D  +IVD+IQDRG NQVYIIGGDGT RGA  I
Sbjct: 143 NTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRGASVI 202

Query: 201 FEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVNGIGL 260
           FEE+ RR L+VAV GIPKT+DNDI +ID+SFGF TAVE AQ+AI+AAHVEAES  NGIG 
Sbjct: 203 FEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESNENGIGF 262

Query: 261 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVLVVAE 320
           VKLMGR +G+IA++ATL+SRDVDCCLIPE  FYLEG+GGLF F+ERRLK++GH V+V+AE
Sbjct: 263 VKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGHMVIVLAE 322

Query: 321 GAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDPTYMI 380
           GAGQD + ++  +    D SGN +  DVG WL   +KD + +     +  +KYIDPTYMI
Sbjct: 323 GAGQDLMCKS-MESTPMDASGNKLLKDVGLWLSQSIKDHFKKNK--MVMNLKYIDPTYMI 382

Query: 381 RAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVNTRDH 440
           RAVP+NA+DN YCTLLA SA+HG MAGYTG+ SG +NG  +YIP   + E +N V   D 
Sbjct: 383 RAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQNNVVITDR 442

Query: 441 KWAWVRSVTNQPDFV 456
            WA + S TNQP F+
Sbjct: 443 MWARLLSSTNQPSFL 451

BLAST of Carg00525 vs. ExPASy Swiss-Prot
Match: Q9M076 (ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE=1 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 4.9e-143
Identity = 249/435 (57.24%), Postives = 320/435 (73.56%), Query Frame = 0

Query: 21  LPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVAYYRA 80
           +PHL+D+   L +  NPLQ+N  +  +  ++    D V  +I   +  K SP    + RA
Sbjct: 24  VPHLSDYILDLPTYPNPLQSNAAYSVVRQYFVDEDDTVQEKI---VVHKDSPRGTHFRRA 83

Query: 81  GPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRGFYSS 140
           GPR+ VYF+P  VRA IVTCGGLCPG+NTVIRE+V GL  +YGV ++ G+  G+RGFYS 
Sbjct: 84  GPRQKVYFKPSDVRACIVTCGGLCPGLNTVIREIVCGLHFMYGVTEVIGVDCGFRGFYSK 143

Query: 141 EPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRGAVKI 200
             V L+ K V D HKRGGT L TSRGG D  +IVD IQDR  NQVYIIGGDGT +GA  I
Sbjct: 144 NTVALTPKTVSDIHKRGGTILGTSRGGHDTSKIVDNIQDREINQVYIIGGDGTQKGANAI 203

Query: 201 FEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVNGIGL 260
           ++E+ RR L+VAV GIPKT+DNDI +ID+SFGF TAVE AQ+AI+AAHVEA S  NGIG+
Sbjct: 204 YKEIRRRGLKVAVAGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEATSVENGIGI 263

Query: 261 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVLVVAE 320
           VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG GGL+ F+ +RL+ENGH V+V+AE
Sbjct: 264 VKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGKGGLYEFIAKRLRENGHMVIVIAE 323

Query: 321 GAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDPTYMI 380
           GAGQD +  +    E++D SGN +  DVG W+  ++K+++A+ +  ++ T+KYIDPTYMI
Sbjct: 324 GAGQDLVAES---IEQQDASGNKLLKDVGLWMSLKIKEYFAKHNVMDI-TLKYIDPTYMI 383

Query: 381 RAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVNTRDH 440
           RA+PANA+DN Y TLLA SA+HG MAGYTGFVSG +NG ++YIP   + E +N+V   D 
Sbjct: 384 RAIPANASDNVYSTLLAQSAVHGAMAGYTGFVSGLVNGRHTYIPFNRITERQNKVVITDR 443

Query: 441 KWAWVRSVTNQPDFV 456
            WA + S TNQP F+
Sbjct: 444 MWARMLSSTNQPSFM 451

BLAST of Carg00525 vs. ExPASy TrEMBL
Match: A0A6J1ISR0 (ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111478077 PE=3 SV=1)

HSP 1 Score: 954.9 bits (2467), Expect = 1.3e-274
Identity = 466/473 (98.52%), Postives = 469/473 (99.15%), Query Frame = 0

Query: 1   MAT-TAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVL 60
           MAT TAAAEI+DRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVL
Sbjct: 1   MATATAAAEISDRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVL 60

Query: 61  RRIAFDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLW 120
           RRIAFDLSRKPSP HVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLW
Sbjct: 61  RRIAFDLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLW 120

Query: 121 ELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQD 180
           ELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQD
Sbjct: 121 ELYGVRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQD 180

Query: 181 RGFNQVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEM 240
           RGFNQVYIIGGDGTMRGAVKIFEEVCRR LQVAVTGIPKTVDNDIGIIDRSFGFQTAVEM
Sbjct: 181 RGFNQVYIIGGDGTMRGAVKIFEEVCRRELQVAVTGIPKTVDNDIGIIDRSFGFQTAVEM 240

Query: 241 AQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG 300
           AQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG
Sbjct: 241 AQQAISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGG 300

Query: 301 LFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDW 360
           LFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDW
Sbjct: 301 LFPFLERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDW 360

Query: 361 WARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGN 420
           WARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGN
Sbjct: 361 WARTHPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGN 420

Query: 421 YSYIPLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKTRLFDPENQSSSGISS 473
           YSYIPLKDVAEAKNEVNT+DHKWAWVRSVTNQPDFV+TRLFDP NQSSSGISS
Sbjct: 421 YSYIPLKDVAEAKNEVNTKDHKWAWVRSVTNQPDFVETRLFDPGNQSSSGISS 473

BLAST of Carg00525 vs. ExPASy TrEMBL
Match: A0A6J1GG28 (ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC111453612 PE=3 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.1e-274
Identity = 464/469 (98.93%), Postives = 466/469 (99.36%), Query Frame = 0

Query: 4   TAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIA 63
           TAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIA
Sbjct: 3   TAAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIA 62

Query: 64  FDLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 123
           FDLSRKPSP HVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG
Sbjct: 63  FDLSRKPSPAHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYG 122

Query: 124 VRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFN 183
           VRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFN
Sbjct: 123 VRQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFN 182

Query: 184 QVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 243
           QVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA
Sbjct: 183 QVYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQA 242

Query: 244 ISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPF 303
           ISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPF
Sbjct: 243 ISAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPF 302

Query: 304 LERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWART 363
           LERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWART
Sbjct: 303 LERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWART 362

Query: 364 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 423
           HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI
Sbjct: 363 HPGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYI 422

Query: 424 PLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKTRLFDPENQSSSGISS 473
           PLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFV+TRLF P N+SSSGISS
Sbjct: 423 PLKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVETRLFVPGNRSSSGISS 471

BLAST of Carg00525 vs. ExPASy TrEMBL
Match: A0A1S3B325 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103485214 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 3.2e-246
Identity = 413/453 (91.17%), Postives = 436/453 (96.25%), Query Frame = 0

Query: 5   AAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAF 64
           A A+I+D+HP IAVA LPHLTD+FP L++RTNPLQ NQFFHPIDGFYATSTD+VLRRIAF
Sbjct: 2   ATAKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIAF 61

Query: 65  DLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 124
           DLS   SP HVAY RAGPRR+VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV
Sbjct: 62  DLSGNTSPAHVAYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 121

Query: 125 RQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQ 184
           R+I+GIVAGYRGFYS EPVEL+LKLVD+WHKRGGTALRTSRGGFDL++IVDAI++ GFNQ
Sbjct: 122 RRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGFNQ 181

Query: 185 VYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 244
           VYI GGDGTMRGAVKIFEE+ RRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI
Sbjct: 182 VYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 241

Query: 245 SAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFL 304
           +AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEG+GGLFPFL
Sbjct: 242 NAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFPFL 301

Query: 305 ERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTH 364
           E+RLKENGHAVLVVAEGAGQ  IPRTEAQKEERDESGNPVFLDVGGWLKTELK+WW RTH
Sbjct: 302 EQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWERTH 361

Query: 365 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 424
           PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP
Sbjct: 362 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 421

Query: 425 LKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKT 458
           LK+VAEAKNEVNTRDHKWAWVRSVTNQPDFVK+
Sbjct: 422 LKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454

BLAST of Carg00525 vs. ExPASy TrEMBL
Match: A0A0A0KL11 (ATP-dependent 6-phosphofructokinase OS=Cucumis sativus OX=3659 GN=PFK PE=3 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 9.4e-246
Identity = 413/453 (91.17%), Postives = 434/453 (95.81%), Query Frame = 0

Query: 5   AAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAF 64
           A A+I+D+HP I VA LPHLTD+FP L++RTNPLQ NQFFHPIDGFYATSTD+VLRRIAF
Sbjct: 2   ATAKISDQHPTITVADLPHLTDNFPNLKTRTNPLQTNQFFHPIDGFYATSTDIVLRRIAF 61

Query: 65  DLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 124
           DLS   SP HVAY RAGPRR VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV
Sbjct: 62  DLSGDTSPAHVAYRRAGPRRKVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 121

Query: 125 RQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQ 184
           RQI+GIVAGYRGFYS+EPVEL+LKLVD+WHKRGGTAL+TSRGGFDL++IVDAI++ GFNQ
Sbjct: 122 RQIYGIVAGYRGFYSTEPVELNLKLVDNWHKRGGTALQTSRGGFDLKKIVDAIENHGFNQ 181

Query: 185 VYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 244
           VYI GGDGTMRGAVKIFEEV RRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI
Sbjct: 182 VYITGGDGTMRGAVKIFEEVRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 241

Query: 245 SAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFL 304
           +AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM FYLEG+GGLFPFL
Sbjct: 242 NAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMAFYLEGNGGLFPFL 301

Query: 305 ERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTH 364
           ERRLKENGHAVLVVAEGAGQ  IPRTEAQKEERDESGNPVFLDVGGWLKTELK+WWA TH
Sbjct: 302 ERRLKENGHAVLVVAEGAGQGMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWATTH 361

Query: 365 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 424
           PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP
Sbjct: 362 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 421

Query: 425 LKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKT 458
           LK+VAEAKNEVNTRDHKWAWVRSVTNQPDFVK+
Sbjct: 422 LKEVAEAKNEVNTRDHKWAWVRSVTNQPDFVKS 454

BLAST of Carg00525 vs. ExPASy TrEMBL
Match: A0A5D3CPV9 (ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PFK PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.2e-245
Identity = 412/453 (90.95%), Postives = 435/453 (96.03%), Query Frame = 0

Query: 5   AAAEIADRHPPIAVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAF 64
           A A+I+D+HP IAVA LPHLTD+FP L++RTNPLQ NQFFHPIDGFYATSTD+VLRRIAF
Sbjct: 2   ATAKISDQHPLIAVADLPHLTDNFPNLETRTNPLQTNQFFHPIDGFYATSTDIVLRRIAF 61

Query: 65  DLSRKPSPGHVAYYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 124
           DLS   SP HVAY RAGPRR+VYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV
Sbjct: 62  DLSGNTSPAHVAYRRAGPRRTVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGV 121

Query: 125 RQIHGIVAGYRGFYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQ 184
           R+I+GIVAGYRGFYS EPVEL+LKLVD+WHKRGGTALRTSRGGFDL++IVDAI++ GFNQ
Sbjct: 122 RRIYGIVAGYRGFYSMEPVELNLKLVDNWHKRGGTALRTSRGGFDLKKIVDAIENHGFNQ 181

Query: 185 VYIIGGDGTMRGAVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 244
           VYI GGDGTMRGAVKIFEE+ RRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI
Sbjct: 182 VYITGGDGTMRGAVKIFEEIRRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAI 241

Query: 245 SAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFL 304
           +AAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEG+GGLFPFL
Sbjct: 242 NAAHVEAESAVNGIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGNGGLFPFL 301

Query: 305 ERRLKENGHAVLVVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTH 364
           E+RLKENGHAVLVVAEGAGQ  IPRTEAQKEERDESGNPVFLDVGGWLKTELK+WW RTH
Sbjct: 302 EQRLKENGHAVLVVAEGAGQRMIPRTEAQKEERDESGNPVFLDVGGWLKTELKNWWERTH 361

Query: 365 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 424
           PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP
Sbjct: 362 PGELFTVKYIDPTYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIP 421

Query: 425 LKDVAEAKNEVNTRDHKWAWVRSVTNQPDFVKT 458
           LK+VAEAKNEVNTRDHKWAWVRSV NQPDFVK+
Sbjct: 422 LKEVAEAKNEVNTRDHKWAWVRSVANQPDFVKS 454

BLAST of Carg00525 vs. TAIR 10
Match: AT5G47810.1 (phosphofructokinase 2 )

HSP 1 Score: 700.7 bits (1807), Expect = 8.2e-202
Identity = 335/438 (76.48%), Postives = 379/438 (86.53%), Query Frame = 0

Query: 17  AVASLPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVA 76
           ++  LP L+     L+ R NPL++N +FHP +GFY T +DV+L ++A+D S   S   VA
Sbjct: 6   SIRKLPSLSG----LRHRRNPLEDNPYFHPSNGFYITPSDVILAQVAYDHSAH-SQSRVA 65

Query: 77  YYRAGPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRG 136
           Y+RAGPRR + +EP +V+AAIVTCGGLCPGMNTVIRELVVGLWELYGVR+I+GI AGYRG
Sbjct: 66  YHRAGPRREIMYEPSAVKAAIVTCGGLCPGMNTVIRELVVGLWELYGVREIYGIPAGYRG 125

Query: 137 FYSSEPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRG 196
           FYS + V+L  K V DWHK+GGT L TSRGGF L++IVDAI   G+NQVYIIGGDGTMRG
Sbjct: 126 FYSMKAVKLDPKAVHDWHKKGGTVLATSRGGFHLQKIVDAIHLNGYNQVYIIGGDGTMRG 185

Query: 197 AVKIFEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVN 256
           AV+IF+E+  R L+V +T IPKTVDND+GIIDRSFGFQTAVEMAQ+AISAAHVEAESAVN
Sbjct: 186 AVEIFKEISLRKLEVGITVIPKTVDNDVGIIDRSFGFQTAVEMAQEAISAAHVEAESAVN 245

Query: 257 GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVL 316
           GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEM+FYLEG GGLF FLE+RLKE GHAVL
Sbjct: 246 GIGLVKLMGRSTGHIALHATLSSRDVDCCLIPEMDFYLEGKGGLFEFLEKRLKERGHAVL 305

Query: 317 VVAEGAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDP 376
           VVAEGAGQ+ IPR E+QK+ERDESGN VFLDVG W K+ LK WW R HP ELFTVKYIDP
Sbjct: 306 VVAEGAGQEMIPRNESQKQERDESGNAVFLDVGVWFKSVLKAWWEREHPDELFTVKYIDP 365

Query: 377 TYMIRAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVN 436
           TYMIRAVPANATDN YCTLLAHSAIHGVMAGYTGFV GPINGNY+YIPL++VA+ KN+VN
Sbjct: 366 TYMIRAVPANATDNLYCTLLAHSAIHGVMAGYTGFVPGPINGNYAYIPLEEVAQTKNQVN 425

Query: 437 TRDHKWAWVRSVTNQPDF 455
           TRDHKWAWVRSVTNQPDF
Sbjct: 426 TRDHKWAWVRSVTNQPDF 438

BLAST of Carg00525 vs. TAIR 10
Match: AT4G26270.1 (phosphofructokinase 3 )

HSP 1 Score: 519.2 bits (1336), Expect = 3.4e-147
Identity = 257/451 (56.98%), Postives = 327/451 (72.51%), Query Frame = 0

Query: 21  LPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVAYYRA 80
           +PHL+D+ P L +  NPLQ+N  +  +  ++  + D V ++I   +  K  P  + + RA
Sbjct: 23  VPHLSDYLPGLPTYPNPLQDNPAYSVVKQYFVDADDSVPQKI---VVHKDGPRGIHFRRA 82

Query: 81  GPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRGFYSS 140
           GPR+ VYFE + V A IVTCGGLCPG+NTVIRE+V  L  +YGV++I GI  GYRGFY+ 
Sbjct: 83  GPRQKVYFESDEVHACIVTCGGLCPGLNTVIREIVSSLSYMYGVKRILGIDGGYRGFYAK 142

Query: 141 EPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRGAVKI 200
             V L  K+V+D HKRGGT L TSRGG D  +IVD+IQDRG NQVYIIGGDGT RGA  I
Sbjct: 143 NTVSLDSKVVNDIHKRGGTILGTSRGGHDTTKIVDSIQDRGINQVYIIGGDGTQRGASVI 202

Query: 201 FEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVNGIGL 260
           FEE+ RR L+VAV GIPKT+DNDI +ID+SFGF TAVE AQ+AI+AAHVEAES  NGIG+
Sbjct: 203 FEEIRRRGLKVAVIGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESIENGIGV 262

Query: 261 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVLVVAE 320
           VKLMGR +G IA++ATL+SRDVDCCLIPE  FYLEG+GGLF ++E+RLKE+GH VLV+AE
Sbjct: 263 VKLMGRYSGFIAMYATLASRDVDCCLIPESPFYLEGEGGLFEYIEKRLKESGHMVLVIAE 322

Query: 321 GAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDPTYMI 380
           GAGQD + ++      +D SGN +  DVG WL   +KD +       +  +KYIDPTYMI
Sbjct: 323 GAGQDLMSKSMESMTLKDASGNKLLKDVGLWLSQSIKDHF--NQKKMVMNLKYIDPTYMI 382

Query: 381 RAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVNTRDH 440
           RAVP+NA+DN YCTLLA SA+HG MAGYTG++SG +NG  +YIP   + E +N V   D 
Sbjct: 383 RAVPSNASDNVYCTLLAQSAVHGAMAGYTGYISGLVNGRQTYIPFYRITEKQNHVVITDR 442

Query: 441 KWAWVRSVTNQPDFVKTR-LFDPENQSSSGI 471
            WA + S TNQP F+  + +FD + +  S +
Sbjct: 443 MWARLLSSTNQPSFLGPKDVFDNKEKPMSAL 468

BLAST of Carg00525 vs. TAIR 10
Match: AT5G61580.1 (phosphofructokinase 4 )

HSP 1 Score: 515.8 bits (1327), Expect = 3.7e-146
Identity = 253/435 (58.16%), Postives = 315/435 (72.41%), Query Frame = 0

Query: 21  LPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVAYYRA 80
           +PHLT   P L S  NPL+ +Q +  +   + +S DVV + I   + +K S   V + RA
Sbjct: 74  VPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNI---VVQKGSKRGVHFRRA 133

Query: 81  GPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRGFYSS 140
           GPR  VYF  + V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  GYRGFYS 
Sbjct: 134 GPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGYRGFYSK 193

Query: 141 EPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRGAVKI 200
             + L+ K+V+D HKRGGT L+TSRGG D  +IVD IQDRG NQVYIIGG GT +GA KI
Sbjct: 194 NTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAEKI 253

Query: 201 FEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVNGIGL 260
           +EEV RR LQVAV+GIPKT+DNDI +ID+SFGF TAVE AQ+AI+AAHVE ES  NG+G+
Sbjct: 254 YEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVENGVGI 313

Query: 261 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVLVVAE 320
           VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F+E RLKEN H V+V+AE
Sbjct: 314 VKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHMVIVIAE 373

Query: 321 GAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDPTYMI 380
           GAGQD + ++    E +D SGN + LDVG WL  ++KD +       +  +KYIDPTYMI
Sbjct: 374 GAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKYIDPTYMI 433

Query: 381 RAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVNTRDH 440
           RA+P+NA+DN YCTLLA SA+HG MAGY+GF  GP+N  ++YIP+  V E  N V   D 
Sbjct: 434 RAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPISQVTEVTNTVKLTDR 493

Query: 441 KWAWVRSVTNQPDFV 456
            WA + + TNQP F+
Sbjct: 494 MWARLLASTNQPSFL 504

BLAST of Carg00525 vs. TAIR 10
Match: AT5G56630.1 (phosphofructokinase 7 )

HSP 1 Score: 513.1 bits (1320), Expect = 2.4e-145
Identity = 252/435 (57.93%), Postives = 318/435 (73.10%), Query Frame = 0

Query: 21  LPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVAYYRA 80
           +PHL D+ P L +  NPLQ+N  +  +  ++  + D V  ++   +  K  P  V + RA
Sbjct: 23  VPHLIDYLPDLPTYPNPLQDNPAYSVVKQYFVHADDSVPEKV---VVHKDGPRGVHFRRA 82

Query: 81  GPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRGFYSS 140
           GPR+ VYFE + V A IVTCGGLCPG+NTVIRE+V  L  +YGV++I GI  GYRGFY+ 
Sbjct: 83  GPRQKVYFESDEVHACIVTCGGLCPGLNTVIREVVSSLSYMYGVKRILGIDGGYRGFYAK 142

Query: 141 EPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRGAVKI 200
             + L+ K+V+D HKRGGT + TSRGG D  +IVD+IQDRG NQVYIIGGDGT RGA  I
Sbjct: 143 NTIPLNSKVVNDIHKRGGTIIGTSRGGHDTNKIVDSIQDRGINQVYIIGGDGTQRGASVI 202

Query: 201 FEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVNGIGL 260
           FEE+ RR L+VAV GIPKT+DNDI +ID+SFGF TAVE AQ+AI+AAHVEAES  NGIG 
Sbjct: 203 FEEIRRRRLKVAVVGIPKTIDNDIPVIDKSFGFDTAVEEAQRAINAAHVEAESNENGIGF 262

Query: 261 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVLVVAE 320
           VKLMGR +G+IA++ATL+SRDVDCCLIPE  FYLEG+GGLF F+ERRLK++GH V+V+AE
Sbjct: 263 VKLMGRYSGYIAMYATLASRDVDCCLIPESPFYLEGEGGLFEFIERRLKDHGHMVIVLAE 322

Query: 321 GAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDPTYMI 380
           GAGQD + ++  +    D SGN +  DVG WL   +KD + +     +  +KYIDPTYMI
Sbjct: 323 GAGQDLMCKS-MESTPMDASGNKLLKDVGLWLSQSIKDHFKKNK--MVMNLKYIDPTYMI 382

Query: 381 RAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVNTRDH 440
           RAVP+NA+DN YCTLLA SA+HG MAGYTG+ SG +NG  +YIP   + E +N V   D 
Sbjct: 383 RAVPSNASDNVYCTLLAQSAVHGAMAGYTGYTSGLVNGRQTYIPFYRITETQNNVVITDR 442

Query: 441 KWAWVRSVTNQPDFV 456
            WA + S TNQP F+
Sbjct: 443 MWARLLSSTNQPSFL 451

BLAST of Carg00525 vs. TAIR 10
Match: AT5G61580.2 (phosphofructokinase 4 )

HSP 1 Score: 510.8 bits (1314), Expect = 1.2e-144
Identity = 253/435 (58.16%), Postives = 315/435 (72.41%), Query Frame = 0

Query: 21  LPHLTDHFPTLQSRTNPLQNNQFFHPIDGFYATSTDVVLRRIAFDLSRKPSPGHVAYYRA 80
           +PHLT   P L S  NPL+ +Q +  +   + +S DVV + I   + +K S   V + RA
Sbjct: 74  VPHLTKFLPDLPSYPNPLKESQAYAIVKRTFVSSEDVVAQNI---VVQKGSKRGVHFRRA 133

Query: 81  GPRRSVYFEPESVRAAIVTCGGLCPGMNTVIRELVVGLWELYGVRQIHGIVAGYRGFYSS 140
           GPR  VYF  + V+A IVTCGGLCPG+NTVIRE+V GL  +YGV  I GI  GYRGFYS 
Sbjct: 134 GPRERVYFRSDEVKACIVTCGGLCPGINTVIREIVCGLNNMYGVNNILGIQGGYRGFYSK 193

Query: 141 EPVELSLKLVDDWHKRGGTALRTSRGGFDLRRIVDAIQDRGFNQVYIIGGDGTMRGAVKI 200
             + L+ K+V+D HKRGGT L+TSRGG D  +IVD IQDRG NQVYIIGG GT +GA KI
Sbjct: 194 NTMNLTPKVVNDIHKRGGTFLQTSRGGHDTAKIVDNIQDRGINQVYIIGGGGTQKGAEKI 253

Query: 201 FEEVCRRNLQVAVTGIPKTVDNDIGIIDRSFGFQTAVEMAQQAISAAHVEAESAVNGIGL 260
           +EEV RR LQVAV+GIPKT+DNDI +ID+SFGF TAVE AQ+AI+AAHVE ES  NG+G+
Sbjct: 254 YEEVERRGLQVAVSGIPKTIDNDIAVIDKSFGFDTAVEEAQRAINAAHVEVESVENGVGI 313

Query: 261 VKLMGRSTGHIALHATLSSRDVDCCLIPEMEFYLEGDGGLFPFLERRLKENGHAVLVVAE 320
           VKLMGR +G IA+ ATL++RDVDCCLIPE  F+LEG GGLF F+E RLKEN H V+V+AE
Sbjct: 314 VKLMGRYSGFIAMIATLANRDVDCCLIPESPFFLEGKGGLFEFIEERLKENRHMVIVIAE 373

Query: 321 GAGQDTIPRTEAQKEERDESGNPVFLDVGGWLKTELKDWWARTHPGELFTVKYIDPTYMI 380
           GAGQD + ++    E +D SGN + LDVG WL  ++KD +       +  +KYIDPTYMI
Sbjct: 374 GAGQDYVAQSMRASETKDASGNRLLLDVGLWLTQQIKDHFTNVRK-MMINMKYIDPTYMI 433

Query: 381 RAVPANATDNSYCTLLAHSAIHGVMAGYTGFVSGPINGNYSYIPLKDVAEAKNEVNTRDH 440
           RA+P+NA+DN YCTLLA SA+HG MAGY+GF  GP+N  ++YIP+  V E  N V   D 
Sbjct: 434 RAIPSNASDNVYCTLLAQSAVHGAMAGYSGFTVGPVNSRHAYIPI-SVTEVTNTVKLTDR 493

Query: 441 KWAWVRSVTNQPDFV 456
            WA + + TNQP F+
Sbjct: 494 MWARLLASTNQPSFL 503

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6603845.14.6e-279100.00ATP-dependent 6-phosphofructokinase 2, partial [Cucurbita argyrosperma subsp. so... [more]
XP_023544772.11.5e-27799.36ATP-dependent 6-phosphofructokinase 2 [Cucurbita pepo subsp. pepo][more]
XP_022977934.12.6e-27498.52ATP-dependent 6-phosphofructokinase 2 [Cucurbita maxima][more]
XP_022950529.14.4e-27498.93ATP-dependent 6-phosphofructokinase 2 [Cucurbita moschata][more]
XP_038881531.11.1e-24891.83ATP-dependent 6-phosphofructokinase 2 isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q9FIK01.2e-20076.48ATP-dependent 6-phosphofructokinase 2 OS=Arabidopsis thaliana OX=3702 GN=PFK2 PE... [more]
Q94AA44.8e-14656.98ATP-dependent 6-phosphofructokinase 3 OS=Arabidopsis thaliana OX=3702 GN=PFK3 PE... [more]
Q9FKG35.3e-14558.16ATP-dependent 6-phosphofructokinase 4, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9C5J73.4e-14457.93ATP-dependent 6-phosphofructokinase 7 OS=Arabidopsis thaliana OX=3702 GN=PFK7 PE... [more]
Q9M0764.9e-14357.24ATP-dependent 6-phosphofructokinase 6 OS=Arabidopsis thaliana OX=3702 GN=PFK6 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1ISR01.3e-27498.52ATP-dependent 6-phosphofructokinase OS=Cucurbita maxima OX=3661 GN=LOC111478077 ... [more]
A0A6J1GG282.1e-27498.93ATP-dependent 6-phosphofructokinase OS=Cucurbita moschata OX=3662 GN=LOC11145361... [more]
A0A1S3B3253.2e-24691.17ATP-dependent 6-phosphofructokinase OS=Cucumis melo OX=3656 GN=LOC103485214 PE=3... [more]
A0A0A0KL119.4e-24691.17ATP-dependent 6-phosphofructokinase OS=Cucumis sativus OX=3659 GN=PFK PE=3 SV=1[more]
A0A5D3CPV91.2e-24590.95ATP-dependent 6-phosphofructokinase OS=Cucumis melo var. makuwa OX=1194695 GN=PF... [more]
Match NameE-valueIdentityDescription
AT5G47810.18.2e-20276.48phosphofructokinase 2 [more]
AT4G26270.13.4e-14756.98phosphofructokinase 3 [more]
AT5G61580.13.7e-14658.16phosphofructokinase 4 [more]
AT5G56630.12.4e-14557.93phosphofructokinase 7 [more]
AT5G61580.21.2e-14458.16phosphofructokinase 4 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR022953ATP-dependent 6-phosphofructokinasePRINTSPR00476PHFRCTKINASEcoord: 214..231
score: 36.18
coord: 181..197
score: 43.89
coord: 310..322
score: 37.28
coord: 253..269
score: 28.51
coord: 232..250
score: 26.05
coord: 97..116
score: 45.13
NoneNo IPR availableGENE3D3.40.50.450coord: 89..278
e-value: 3.8E-56
score: 191.4
NoneNo IPR availablePANTHERPTHR45770FAMILY NOT NAMEDcoord: 15..457
NoneNo IPR availablePANTHERPTHR45770:SF9ATP-DEPENDENT 6-PHOSPHOFRUCTOKINASE 2coord: 15..457
IPR000023Phosphofructokinase domainPFAMPF00365PFKcoord: 95..402
e-value: 3.0E-55
score: 187.6
IPR012004Pyrophosphate-dependent phosphofructokinase TP0108-typePIRSFPIRSF000534ATP_PFK_TP0108coord: 7..459
e-value: 2.4E-172
score: 571.4
IPR012004Pyrophosphate-dependent phosphofructokinase TP0108-typeHAMAPMF_01981Phosphofructokinase_II_Xcoord: 21..455
score: 51.573387
IPR035966Phosphofructokinase superfamilySUPERFAMILY53784Phosphofructokinasecoord: 25..457

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg00525-RACarg00525-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006002 fructose 6-phosphate metabolic process
biological_process GO:0061615 glycolytic process through fructose-6-phosphate
biological_process GO:0006096 glycolytic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003872 6-phosphofructokinase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0046872 metal ion binding