CaUC07G134270 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC07G134270
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
Description9-cis-epoxycarotenoid dioxygenase
LocationCiama_Chr07: 24220560 .. 24222347 (+)
RNA-Seq ExpressionCaUC07G134270
SyntenyCaUC07G134270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTCTTTCTTCTTCTTCATGGATCAAATCTGGTTTTTCCTCTTCAATGCCGGAGAATCTCTCTGTAAATACAAATTATGGTAGAAACAGAGTTGCTACCTCCTGTTCTCTTCATACGCCTTCTATTATTCAAATTCCTAAGCATTCTCACACCGCATTTCCATCTCCTATTAAGACTGCAGTGCCTAAACCCCTTCCAGTTTCGCCTCCCTCCCTGTCCTCCGCATCCGAGGACTGGAATTTCTTGCAGCGGGCTGCGGCTATGGCTCTTGATGCGGTTGAAAATGCTCTGGTTTCGGCAGAGCGGAAGCATTCCTTGCCCAAGACCGCCGACCCTGCGGTTCAAATTGCTGGCAATTTTGCCCCGGTGCCGGAGCAGCCGGTTCGGAAGGGCTTACCGGTGATTGGGAAAGTTCCTGACTGTATTCGAGGGGTTTATGTGCGAAACGGGGCCAACCCACTTCACGAACCCATGTCCGGTCATCATTTGTTCGACGGTGATGGGATGGTTCACGCTGTGGAATTCTCTGAGGGCGGCGGTGTTAGTTACGCTTGCCGGTTCACGGAGACCCAACGGCTGGTTCAGGAGCGAGCTTACGGCCGGCCGGTATTCCCAAAGGCGATCGGCGAACTCCATGGTCACTCTGGGATAGCTCGGCTGATGCTTTTCTATGCCAGAGGATTATTTGGCCTTGTCGATCACAACCATGGAATTGGAGTCGCTAACGCCGGTCTGGTCTTTTTCAACGGCCGGCTTCTTGCCATGTCGGAGGACGATTTGCCTTACCAAATCAGAGTCACTCCGGCGGGTGATTTGAAAACCGTCGGCCGGTTCGATTTCGATGGCCAACTGACATCCACAATGATAGCCCACCCGAAACTCGACCCTGTTTCCGGCGAGATGTTCGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGAGAGAAATCACCGGACGTTGAAATTCCCCTGCCTCAGCCTACGATGATGCACGATTTCGCAATTACAGAGAAATTCGTCGTAATTCCCGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGCGGGTCTCCGGTGGTATACGACAAAGAAAAAACCTCCCGATTCGGAATTTTGGACAAAAATGCCACAGACGCCAATTCTATTAAATGGATTGAAGCTCCTGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAAACTAACGAAATCGTCGTAATTGGGTCGTGCATGACGCCGCCAGACTCCATTTTCAACGAATGCGAAGAGAATTTGAAATCCGTTTTGTCGGAAATTCGTCTGAATCTCTCGACTGGAAAATCGACCCGTCGACCAATCATCACTGAAACAGAGCAAGTGAACTTAGAGGCCGGAATGGTGAACCGAAATCTACTGGGGAGAAAAACCCAGTTTGCTTATCTTGCTCTAGCAGAGCCATGGCCAAAAGTTTCTGGTTTTGCGAAGGTCGATCTCTTCACCGGAGAAATCAAAAAGTATCTCTATGGCGAACAGAGGTACGGCGGTGAGCCTCTGTTCCTGCCTAGAGAAGGGGCTGAGGCAGAGGACGATGGCCACATCTTGGCCTTCGTTCACGACGAGAAGGAATGGAAATCGGAGCTTCAGATCGTTAACGCCATGACTTTGGAGCTTGAAGCTACTGTAAAGCTGCCTTCTCGAGTTCCTTATGGCTTCCATGGAACTTTCATAAGTTCCGAGGATTTGCAGAAGCAGATACGGTAA

mRNA sequence

ATGGCTTCTCTTTCTTCTTCTTCATGGATCAAATCTGGTTTTTCCTCTTCAATGCCGGAGAATCTCTCTGTAAATACAAATTATGGTAGAAACAGAGTTGCTACCTCCTGTTCTCTTCATACGCCTTCTATTATTCAAATTCCTAAGCATTCTCACACCGCATTTCCATCTCCTATTAAGACTGCAGTGCCTAAACCCCTTCCAGTTTCGCCTCCCTCCCTGTCCTCCGCATCCGAGGACTGGAATTTCTTGCAGCGGGCTGCGGCTATGGCTCTTGATGCGGTTGAAAATGCTCTGGTTTCGGCAGAGCGGAAGCATTCCTTGCCCAAGACCGCCGACCCTGCGGTTCAAATTGCTGGCAATTTTGCCCCGGTGCCGGAGCAGCCGGTTCGGAAGGGCTTACCGGTGATTGGGAAAGTTCCTGACTGTATTCGAGGGGTTTATGTGCGAAACGGGGCCAACCCACTTCACGAACCCATGTCCGGTCATCATTTGTTCGACGGTGATGGGATGGTTCACGCTGTGGAATTCTCTGAGGGCGGCGGTGTTAGTTACGCTTGCCGGTTCACGGAGACCCAACGGCTGGTTCAGGAGCGAGCTTACGGCCGGCCGGTATTCCCAAAGGCGATCGGCGAACTCCATGGTCACTCTGGGATAGCTCGGCTGATGCTTTTCTATGCCAGAGGATTATTTGGCCTTGTCGATCACAACCATGGAATTGGAGTCGCTAACGCCGGTCTGGTCTTTTTCAACGGCCGGCTTCTTGCCATGTCGGAGGACGATTTGCCTTACCAAATCAGAGTCACTCCGGCGGGTGATTTGAAAACCGTCGGCCGGTTCGATTTCGATGGCCAACTGACATCCACAATGATAGCCCACCCGAAACTCGACCCTGTTTCCGGCGAGATGTTCGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGAGAGAAATCACCGGACGTTGAAATTCCCCTGCCTCAGCCTACGATGATGCACGATTTCGCAATTACAGAGAAATTCGTCGTAATTCCCGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGCGGGTCTCCGGTGGTATACGACAAAGAAAAAACCTCCCGATTCGGAATTTTGGACAAAAATGCCACAGACGCCAATTCTATTAAATGGATTGAAGCTCCTGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAAACTAACGAAATCGTCGTAATTGGGTCGTGCATGACGCCGCCAGACTCCATTTTCAACGAATGCGAAGAGAATTTGAAATCCGTTTTGTCGGAAATTCGTCTGAATCTCTCGACTGGAAAATCGACCCGTCGACCAATCATCACTGAAACAGAGCAAGTGAACTTAGAGGCCGGAATGGTGAACCGAAATCTACTGGGGAGAAAAACCCAGTTTGCTTATCTTGCTCTAGCAGAGCCATGGCCAAAAGTTTCTGGTTTTGCGAAGGTCGATCTCTTCACCGGAGAAATCAAAAAGTATCTCTATGGCGAACAGAGGTACGGCGGTGAGCCTCTGTTCCTGCCTAGAGAAGGGGCTGAGGCAGAGGACGATGGCCACATCTTGGCCTTCGTTCACGACGAGAAGGAATGGAAATCGGAGCTTCAGATCGTTAACGCCATGACTTTGGAGCTTGAAGCTACTGTAAAGCTGCCTTCTCGAGTTCCTTATGGCTTCCATGGAACTTTCATAAGTTCCGAGGATTTGCAGAAGCAGATACGGTAA

Coding sequence (CDS)

ATGGCTTCTCTTTCTTCTTCTTCATGGATCAAATCTGGTTTTTCCTCTTCAATGCCGGAGAATCTCTCTGTAAATACAAATTATGGTAGAAACAGAGTTGCTACCTCCTGTTCTCTTCATACGCCTTCTATTATTCAAATTCCTAAGCATTCTCACACCGCATTTCCATCTCCTATTAAGACTGCAGTGCCTAAACCCCTTCCAGTTTCGCCTCCCTCCCTGTCCTCCGCATCCGAGGACTGGAATTTCTTGCAGCGGGCTGCGGCTATGGCTCTTGATGCGGTTGAAAATGCTCTGGTTTCGGCAGAGCGGAAGCATTCCTTGCCCAAGACCGCCGACCCTGCGGTTCAAATTGCTGGCAATTTTGCCCCGGTGCCGGAGCAGCCGGTTCGGAAGGGCTTACCGGTGATTGGGAAAGTTCCTGACTGTATTCGAGGGGTTTATGTGCGAAACGGGGCCAACCCACTTCACGAACCCATGTCCGGTCATCATTTGTTCGACGGTGATGGGATGGTTCACGCTGTGGAATTCTCTGAGGGCGGCGGTGTTAGTTACGCTTGCCGGTTCACGGAGACCCAACGGCTGGTTCAGGAGCGAGCTTACGGCCGGCCGGTATTCCCAAAGGCGATCGGCGAACTCCATGGTCACTCTGGGATAGCTCGGCTGATGCTTTTCTATGCCAGAGGATTATTTGGCCTTGTCGATCACAACCATGGAATTGGAGTCGCTAACGCCGGTCTGGTCTTTTTCAACGGCCGGCTTCTTGCCATGTCGGAGGACGATTTGCCTTACCAAATCAGAGTCACTCCGGCGGGTGATTTGAAAACCGTCGGCCGGTTCGATTTCGATGGCCAACTGACATCCACAATGATAGCCCACCCGAAACTCGACCCTGTTTCCGGCGAGATGTTCGCTTTAAGCTACGACGTAATCCAAAAGCCATATTTGAAATACTTCAAATTCTCGCCGGAGGGAGAGAAATCACCGGACGTTGAAATTCCCCTGCCTCAGCCTACGATGATGCACGATTTCGCAATTACAGAGAAATTCGTCGTAATTCCCGACCAGCAGGTGGTTTTCAAGTTGCCGGAGATGATCCGCGGCGGGTCTCCGGTGGTATACGACAAAGAAAAAACCTCCCGATTCGGAATTTTGGACAAAAATGCCACAGACGCCAATTCTATTAAATGGATTGAAGCTCCTGATTGCTTCTGTTTCCATCTCTGGAACGCTTGGGAAGAACCAGAAACTAACGAAATCGTCGTAATTGGGTCGTGCATGACGCCGCCAGACTCCATTTTCAACGAATGCGAAGAGAATTTGAAATCCGTTTTGTCGGAAATTCGTCTGAATCTCTCGACTGGAAAATCGACCCGTCGACCAATCATCACTGAAACAGAGCAAGTGAACTTAGAGGCCGGAATGGTGAACCGAAATCTACTGGGGAGAAAAACCCAGTTTGCTTATCTTGCTCTAGCAGAGCCATGGCCAAAAGTTTCTGGTTTTGCGAAGGTCGATCTCTTCACCGGAGAAATCAAAAAGTATCTCTATGGCGAACAGAGGTACGGCGGTGAGCCTCTGTTCCTGCCTAGAGAAGGGGCTGAGGCAGAGGACGATGGCCACATCTTGGCCTTCGTTCACGACGAGAAGGAATGGAAATCGGAGCTTCAGATCGTTAACGCCATGACTTTGGAGCTTGAAGCTACTGTAAAGCTGCCTTCTCGAGTTCCTTATGGCTTCCATGGAACTTTCATAAGTTCCGAGGATTTGCAGAAGCAGATACGGTAA

Protein sequence

MASLSSSSWIKSGFSSSMPENLSVNTNYGRNRVATSCSLHTPSIIQIPKHSHTAFPSPIKTAVPKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR
Homology
BLAST of CaUC07G134270 vs. NCBI nr
Match: APO14273.1 (9-cis-epoxycarotenoid dioxygenase NCED1 [Luffa aegyptiaca])

HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 563/598 (94.15%), Postives = 580/598 (96.99%), Query Frame = 0

Query: 1   MASL--SSSSWIKSGFSSSMPENLSVNTNYG-RNRVATSCSLHTPSIIQIPKHSHTAFPS 60
           MASL  SSSSW+KS FSSSMPE +SVN NYG +NRVA  CSLHTPSIIQIPKHS TAF S
Sbjct: 1   MASLGSSSSSWVKSRFSSSMPELVSVNRNYGLQNRVA--CSLHTPSIIQIPKHSQTAFRS 60

Query: 61  PIKTAVPKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQ 120
           P+KTAVPKP+PVSPPS+SS SE+WNFLQRAAA+ALDAVE+ALVSAERKHSLPKTADPAVQ
Sbjct: 61  PVKTAVPKPVPVSPPSVSSGSEEWNFLQRAAAVALDAVEDALVSAERKHSLPKTADPAVQ 120

Query: 121 IAGNFAPVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEF 180
           IAGNFAPVPEQPVRK LPV GKVPDCIRGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEF
Sbjct: 121 IAGNFAPVPEQPVRKALPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEF 180

Query: 181 SEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHN 240
           SEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDH+
Sbjct: 181 SEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHS 240

Query: 241 HGIGVANAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLD 300
           HG+GVANAGLV+FNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQL STMIAHPKLD
Sbjct: 241 HGLGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLD 300

Query: 301 PVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQ 360
           PVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQ
Sbjct: 301 PVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQ 360

Query: 361 VVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPET 420
           VVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDAN+IKWIEAPDCFCFHLWNAWEEPET
Sbjct: 361 VVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPET 420

Query: 421 NEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVN 480
           +EIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPII ETEQVNLEAGMVN
Sbjct: 421 DEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVN 480

Query: 481 RNLLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAE 540
           RN LGRKTQFAYLALAEPWPKVSGFAKVDL TGEI+KYLYGEQRYGGEPLFLPREGA AE
Sbjct: 481 RNQLGRKTQFAYLALAEPWPKVSGFAKVDLATGEIRKYLYGEQRYGGEPLFLPREGATAE 540

Query: 541 DDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 596
           DDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISS+DLQKQIR
Sbjct: 541 DDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQIR 596

BLAST of CaUC07G134270 vs. NCBI nr
Match: XP_038878047.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic [Benincasa hispida])

HSP 1 Score: 1130.5 bits (2923), Expect = 0.0e+00
Identity = 557/594 (93.77%), Postives = 571/594 (96.13%), Query Frame = 0

Query: 2   ASLSSSSWIKSGFSSSMPENLSVNTNYGRNRVATSCSLHTPSIIQIPKHSHTAFPSPIKT 61
           +S SSSS +KS  SS M +NLS+N+NYGRNRVA +CSLHTPSIIQIPKHSHTAFPSP+KT
Sbjct: 4   SSSSSSSLLKSPLSSPMLQNLSLNSNYGRNRVAPTCSLHTPSIIQIPKHSHTAFPSPLKT 63

Query: 62  AVPKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGN 121
            VP     SPPSLSS S DWNFLQRAAA+ALDAVENALVSAERKHSLPKTADPAVQIAGN
Sbjct: 64  TVPP----SPPSLSSPSHDWNFLQRAAAIALDAVENALVSAERKHSLPKTADPAVQIAGN 123

Query: 122 FAPVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGG 181
           FAPVPEQPVRKGLPV GKVPD IRGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEFS+GG
Sbjct: 124 FAPVPEQPVRKGLPVTGKVPDSIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSDGG 183

Query: 182 GVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIG 241
            VSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIG
Sbjct: 184 AVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIG 243

Query: 242 VANAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSG 301
           VANAGLVFFNGRLLAMSEDDLPYQI VTPAGDLKTVGRFDF GQLTSTMIAHPKLDPVSG
Sbjct: 244 VANAGLVFFNGRLLAMSEDDLPYQITVTPAGDLKTVGRFDFHGQLTSTMIAHPKLDPVSG 303

Query: 302 EMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFK 361
           EMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFK
Sbjct: 304 EMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFK 363

Query: 362 LPEMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIV 421
           LPEMIRGGSPVVYDKEK SRFGILDKNATDAN+IKWIEAPDCFCFHLWNAWEEPETNEIV
Sbjct: 364 LPEMIRGGSPVVYDKEKISRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNEIV 423

Query: 422 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLL 481
           VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPII ETEQVNLEAGMVNRN L
Sbjct: 424 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNRL 483

Query: 482 GRKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGH 541
           GRKTQFAYLALAEPWPKVSGFAKVDLFTGEI+KYLYG+QRYGGEPLFLPREGAEAEDDGH
Sbjct: 484 GRKTQFAYLALAEPWPKVSGFAKVDLFTGEIRKYLYGDQRYGGEPLFLPREGAEAEDDGH 543

Query: 542 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 596
           ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISS+DLQKQIR
Sbjct: 544 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQIR 593

BLAST of CaUC07G134270 vs. NCBI nr
Match: XP_008465499.1 (PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis melo])

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 547/592 (92.40%), Postives = 569/592 (96.11%), Query Frame = 0

Query: 4   LSSSSWIKSGFSSSMPENLSVNTNYGRNRVATSCSLHTPSIIQIPKHSHTAFPSPIKTAV 63
           ++SSS++KS FSSSMP+  +++    R  ++ S SLHTPSIIQIPKHSHT FPSP+KT++
Sbjct: 1   MASSSFLKSPFSSSMPDK-TLSNYTSRRLLSVSSSLHTPSIIQIPKHSHTTFPSPLKTSI 60

Query: 64  PKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFA 123
           PKP P+S     S SEDWNFLQRAAAMALDAVENAL+SAERKHSLPKTADPAVQIAGNFA
Sbjct: 61  PKPPPLSTTPPLSGSEDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFA 120

Query: 124 PVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGV 183
           PVPEQPVRKGLPVIGKVP+CIRGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEFSE GGV
Sbjct: 121 PVPEQPVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEAGGV 180

Query: 184 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVA 243
           SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHN GIGVA
Sbjct: 181 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNQGIGVA 240

Query: 244 NAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEM 303
           NAGLV+FNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQL STMIAHPKLDPVSGEM
Sbjct: 241 NAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEM 300

Query: 304 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLP 363
           FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVVFKLP
Sbjct: 301 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLP 360

Query: 364 EMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVI 423
           EMIRGGSPVVYDKEKTSRFGILDKNA DAN+IKWIEAPDCFCFHLWNAWEEPETNEIVVI
Sbjct: 361 EMIRGGSPVVYDKEKTSRFGILDKNAADANAIKWIEAPDCFCFHLWNAWEEPETNEIVVI 420

Query: 424 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGR 483
           GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPII+ETEQVNLEAGMVNRNLLGR
Sbjct: 421 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIISETEQVNLEAGMVNRNLLGR 480

Query: 484 KTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHIL 543
           KTQF+YLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHIL
Sbjct: 481 KTQFSYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHIL 540

Query: 544 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 596
           AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR
Sbjct: 541 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 591

BLAST of CaUC07G134270 vs. NCBI nr
Match: XP_004147768.1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic [Cucumis sativus] >KAE8649239.1 hypothetical protein Csa_015296 [Cucumis sativus])

HSP 1 Score: 1110.5 bits (2871), Expect = 0.0e+00
Identity = 545/596 (91.44%), Postives = 571/596 (95.81%), Query Frame = 0

Query: 1   MASLSSSSWIKSGFSSSMPENLSVNTNYGRNR-VATSCSLHTPSIIQIPKHSHTAFPSPI 60
           MAS SSSS+++S FSSSMP+     +NY  NR ++ S SLHTPSIIQIPKHSHT FPSP+
Sbjct: 1   MASSSSSSFLQSPFSSSMPDKKL--SNYTTNRLLSVSSSLHTPSIIQIPKHSHTTFPSPL 60

Query: 61  KTAVPKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIA 120
           +T++PKP P++     S S+DWNFLQRAAAMALDAVENAL+SAERKHSLPKTADPAVQIA
Sbjct: 61  QTSIPKPPPLTTTPPVSGSQDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIA 120

Query: 121 GNFAPVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSE 180
           GNFAPVPEQPVRKGLPVIGKVPD IRGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEFSE
Sbjct: 121 GNFAPVPEQPVRKGLPVIGKVPDHIRGVYVRNGANPLHEPLSGHHLFDGDGMVHAVEFSE 180

Query: 181 GGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHG 240
            GGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHG
Sbjct: 181 AGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHG 240

Query: 241 IGVANAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPV 300
           IGVANAGLV+FNGRLLAMSEDDLPYQIRVTPAGDLKTVGRF+FDGQL STMIAHPKLDPV
Sbjct: 241 IGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFNFDGQLESTMIAHPKLDPV 300

Query: 301 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVV 360
           SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVV
Sbjct: 301 SGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVV 360

Query: 361 FKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNE 420
           FKLPEMIRGGSPVVYDKEKTSRFGILDKNATDAN+IKWIEAPDCFCFHLWNAWEEPETNE
Sbjct: 361 FKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPETNE 420

Query: 421 IVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRN 480
           +VVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRN
Sbjct: 421 VVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRN 480

Query: 481 LLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDD 540
           LLGRKTQF+YLALAEPWPKVSGFAKVD+ +GE+KKYLYGEQRYGGEPLFLPREGAEAEDD
Sbjct: 481 LLGRKTQFSYLALAEPWPKVSGFAKVDVLSGEVKKYLYGEQRYGGEPLFLPREGAEAEDD 540

Query: 541 GHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 596
           GHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFIS +DLQKQIR
Sbjct: 541 GHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISCKDLQKQIR 594

BLAST of CaUC07G134270 vs. NCBI nr
Match: KAA0062986.1 (9-cis-epoxycarotenoid dioxygenase NCED3 [Cucumis melo var. makuwa] >TYK16360.1 9-cis-epoxycarotenoid dioxygenase NCED3 [Cucumis melo var. makuwa])

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 538/571 (94.22%), Postives = 553/571 (96.85%), Query Frame = 0

Query: 26  TNY-GRNRVATSCSLHTPSIIQIPKHSHTAFPSPIKTAVPKPLPVSPPSLSSASEDWNFL 85
           +NY  R  ++ S SLHTPSIIQIPKHSHT FPSP+KT++PKP P+S     S SEDWNFL
Sbjct: 7   SNYTSRRLLSVSSSLHTPSIIQIPKHSHTTFPSPLKTSIPKPPPLSTTPPLSGSEDWNFL 66

Query: 86  QRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVPDCI 145
           QRAAAMALDAVENAL+SAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVP+CI
Sbjct: 67  QRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVPECI 126

Query: 146 RGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAYGRP 205
           RGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEFSE GGVSYACRFTETQRLVQERAYGRP
Sbjct: 127 RGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEAGGVSYACRFTETQRLVQERAYGRP 186

Query: 206 VFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAMSEDDLPY 265
           VFPKAIGELHGHSGIARLMLFYARGLFGLVDHN GIGVANAGLV+FNGRLLAMSEDDLPY
Sbjct: 187 VFPKAIGELHGHSGIARLMLFYARGLFGLVDHNQGIGVANAGLVYFNGRLLAMSEDDLPY 246

Query: 266 QIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPE 325
           QIRVTPAGDLKTVGRFDFDGQL STMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPE
Sbjct: 247 QIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPE 306

Query: 326 GEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGI 385
           GEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGI
Sbjct: 307 GEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGI 366

Query: 386 LDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSV 445
           LDKNA DAN+IKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSV
Sbjct: 367 LDKNAADANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSV 426

Query: 446 LSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQFAYLALAEPWPKVSGFAK 505
           LSEIRLNLSTGKSTRRPII+ETEQVNLEAGMVNRNLLGRKTQF+YLALAEPWPKVSGFAK
Sbjct: 427 LSEIRLNLSTGKSTRRPIISETEQVNLEAGMVNRNLLGRKTQFSYLALAEPWPKVSGFAK 486

Query: 506 VDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAMTL 565
           VDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAMTL
Sbjct: 487 VDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAMTL 546

Query: 566 ELEATVKLPSRVPYGFHGTFISSEDLQKQIR 596
           ELEATVKLPSRVPYGFHGTFISSEDLQKQIR
Sbjct: 547 ELEATVKLPSRVPYGFHGTFISSEDLQKQIR 577

BLAST of CaUC07G134270 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 1.8e-254
Identity = 435/592 (73.48%), Postives = 492/592 (83.11%), Query Frame = 0

Query: 4   LSSSSWIKSGFSSSMPENLSVNTNYGRNRVATSCSLHTPSIIQIPKHSHTAFPSPIKTAV 63
           LSSS      + SS+P    V       ++  S +LHTP  +  PK S  +         
Sbjct: 24  LSSSQSSDLSYCSSLPMASRVT-----RKLNVSSALHTPPALHFPKQSSNS--------- 83

Query: 64  PKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFA 123
              + V P +  S ++  N  QRAAA ALDA E  LVS E+ H LPKTADP+VQIAGNFA
Sbjct: 84  -PAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFA 143

Query: 124 PVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGV 183
           PV EQPVR+ LPV+GK+PD I+GVYVRNGANPLHEP++GHH FDGDGMVHAV+F E G  
Sbjct: 144 PVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EHGSA 203

Query: 184 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVA 243
           SYACRFT+T R VQER  GRPVFPKAIGELHGH+GIARLMLFYAR   G+VD  HG GVA
Sbjct: 204 SYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVA 263

Query: 244 NAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEM 303
           NAGLV+FNGRLLAMSEDDLPYQ+++TP GDLKTVGRFDFDGQL STMIAHPK+DP SGE+
Sbjct: 264 NAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGEL 323

Query: 304 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLP 363
           FALSYDV+ KPYLKYF+FSP+G KSPDVEI L QPTMMHDFAITE FVV+PDQQVVFKLP
Sbjct: 324 FALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLP 383

Query: 364 EMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVI 423
           EMIRGGSPVVYDK K +RFGILDK A D+++IKWI+APDCFCFHLWNAWEEPET+E+VVI
Sbjct: 384 EMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVVI 443

Query: 424 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIT-ETEQVNLEAGMVNRNLLG 483
           GSCMTPPDSIFNE +ENLKSVLSEIRLNL TG+STRRPII+ E +QVNLEAGMVNRN+LG
Sbjct: 444 GSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLG 503

Query: 484 RKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHI 543
           RKT+FAYLALAEPWPKVSGFAKVDL TGE+KK+LYG+ RYGGEPLFLP EG E ED+G+I
Sbjct: 504 RKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGE-EDEGYI 563

Query: 544 LAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQI 595
           L FVHDEK WKSELQIVNA++LE+EATVKLPSRVPYGFHGTFI ++DL KQ+
Sbjct: 564 LCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQV 598

BLAST of CaUC07G134270 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 876.7 bits (2264), Expect = 1.5e-253
Identity = 431/601 (71.71%), Postives = 495/601 (82.36%), Query Frame = 0

Query: 3   SLSSSSWIKSGFSSSMPENLSVNTN--------YGRNRVATSCSLHTPSIIQIPKHSHTA 62
           S ++++WIK+  S    +     +N        + R  +  + SL  P I+  PK S + 
Sbjct: 6   SHATNTWIKTKLSMPSSKEFGFASNSISLLKNQHNRQSLNINSSLQAPPILHFPKQS-SN 65

Query: 63  FPSPIKTAVPKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADP 122
           + +P    +  P   +  S SS++  WN +Q+AAAMALDAVE+AL   E +H LPKTADP
Sbjct: 66  YQTPKNNTISHPKQENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTADP 125

Query: 123 AVQIAGNFAPVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHA 182
            VQI+GNFAPVPE PV + LPV GK+P C++GVYVRNGANPL EP +GHH FDGDGMVHA
Sbjct: 126 RVQISGNFAPVPENPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHA 185

Query: 183 VEFSEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLV 242
           V+F + G  SYACRFTET+RLVQE+A GRPVFPKAIGELHGHSGIARLMLFYARGLFGLV
Sbjct: 186 VQF-KNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLV 245

Query: 243 DHNHGIGVANAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHP 302
           DH+ G GVANAGLV+FN RLLAMSEDDLPY ++VTP GDLKT GRFDFDGQL STMIAHP
Sbjct: 246 DHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHP 305

Query: 303 KLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIP 362
           KLDPVSGE+FALSYDVIQKPYLKYF+FS  GEKS DVEIP+  PTMMHDFAITE FVVIP
Sbjct: 306 KLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIP 365

Query: 363 DQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEE 422
           DQQVVFK+ EMIRGGSPVVYDK K SRFGILDK A D + +KW+E PDCFCFHLWNAWEE
Sbjct: 366 DQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEE 425

Query: 423 PETNEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPII-TETEQVNLEA 482
            ET+EIVVIGSCMTPPDSIFNEC+E LKSVLSEIRLNL TGKSTR+ II    EQVNLEA
Sbjct: 426 AETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEA 485

Query: 483 GMVNRNLLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPRE- 542
           GMVNRN LGRKT++AYLA+AEPWPKVSGFAKV+LFTGE++K++YG+ +YGGEPLFLPR+ 
Sbjct: 486 GMVNRNKLGRKTEYAYLAIAEPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDP 545

Query: 543 GAEAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQK 594
            ++ EDDG+ILAFVHDEKEWKSELQIVNAM+L+LEATVKLPSRVPYGFHGTFI++ DL  
Sbjct: 546 NSKEEDDGYILAFVHDEKEWKSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLAN 604

BLAST of CaUC07G134270 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 3.4e-250
Identity = 419/566 (74.03%), Postives = 481/566 (84.98%), Query Frame = 0

Query: 30  RNRVATSCSLHTPSIIQIPKHSHTAFPSPIKTAVPKPLPVSPPSLSSASEDWNFLQRAAA 89
           +N   T   +H+ +++ +PK ++T              P + P   ++   WNF Q+AAA
Sbjct: 34  KNHTTTKTKIHS-ALLTLPKQNNT--------------PKNQPQFQTS--HWNFFQKAAA 93

Query: 90  MALDAVENALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVPDCIRGVYV 149
            ALD VE+ALVS E ++ LPKTADP VQIAGNFAPVPEQ VR  LPV G +PDCI GVYV
Sbjct: 94  KALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVPEQSVRHNLPVTGTIPDCINGVYV 153

Query: 150 RNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAYGRPVFPKA 209
           RNGANPL EP++GHHLFDGDGMVHAV   E G VSY+CRFTET+RLVQER  G PVFPKA
Sbjct: 154 RNGANPLFEPVAGHHLFDGDGMVHAVTV-ENGSVSYSCRFTETERLVQERELGHPVFPKA 213

Query: 210 IGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAMSEDDLPYQIRVT 269
           IGELHGHSGIARL+LFYARG+FGLVDH+HG GVANAGLVFFN RLLAMSEDD+PY ++V 
Sbjct: 214 IGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAGLVFFNNRLLAMSEDDVPYHVQVL 273

Query: 270 PAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSP 329
           P+GDL+TVGR++FD QL STMIAHPK+DPVSGE+FALSYDV+QKPYLK FKFSP+GEKSP
Sbjct: 274 PSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFALSYDVVQKPYLKSFKFSPDGEKSP 333

Query: 330 DVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNA 389
           DVEIPL  PTMMHDFAITE +VVIPDQQVVFKL EMI+GGSPV+YDK K SRFGIL KNA
Sbjct: 334 DVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMIKGGSPVIYDKNKKSRFGILPKNA 393

Query: 390 TDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSVLSEIR 449
            ++ +I W+E+ + FCFHLWNAWEEPET+E++VIGSCMTPPDSIFNEC ENLKSVLSEIR
Sbjct: 394 ENSENIIWVESAETFCFHLWNAWEEPETDEVIVIGSCMTPPDSIFNECNENLKSVLSEIR 453

Query: 450 LNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQFAYLALAEPWPKVSGFAKVDLFT 509
           LNL TG+STRR +++ ++QVNLEAGMVNRN LGRKTQFAYLA+AEPWPKVSGFAKVDL T
Sbjct: 454 LNLKTGESTRRQLLSPSDQVNLEAGMVNRNKLGRKTQFAYLAIAEPWPKVSGFAKVDLST 513

Query: 510 GEIKKYLYGEQRYGGEPLFLPRE-GAEAEDDGHILAFVHDEKEWKSELQIVNAMTLELEA 569
           GEIKK++YG++RYGGEPLFLPR   +E EDDG+ILAF HDEK WKSELQIVNAMTLELEA
Sbjct: 514 GEIKKHIYGDKRYGGEPLFLPRNVNSEKEDDGYILAFCHDEKTWKSELQIVNAMTLELEA 573

Query: 570 TVKLPSRVPYGFHGTFISSEDLQKQI 595
           TVKLPSRVPYGFHGTFISS+DLQ Q+
Sbjct: 574 TVKLPSRVPYGFHGTFISSKDLQNQV 581

BLAST of CaUC07G134270 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 864.8 bits (2233), Expect = 5.8e-250
Identity = 434/617 (70.34%), Postives = 493/617 (79.90%), Query Frame = 0

Query: 1   MASLSSSSWIK--------------SGFSSSMPENLSVNTNYGRNRVATSCS---LHTPS 60
           M S +S++WI               +  SSS    L        N    +CS   LH P 
Sbjct: 1   MPSPASNTWINTTLPSSCSSPFKDLASTSSSPTTLLPFKKRSSSNTNTITCSLQTLHYPK 60

Query: 61  IIQIPKHSHTAFPSPIK----TAVPKPLPVSPPSLSSAS---EDWNFLQRAAAMALDAVE 120
             Q    S T  P+PIK    T    P   + P   +     + WNFLQ+AAA  LD VE
Sbjct: 61  QYQPTSTSTTTTPTPIKPTTTTTTTTPHRETKPLSDTKQPFPQKWNFLQKAAATGLDMVE 120

Query: 121 NALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPL 180
            ALVS E KH LPKTADP VQIAGNFAPVPE    + LPV+GK+P CI GVYVRNGANPL
Sbjct: 121 TALVSHESKHPLPKTADPKVQIAGNFAPVPEHAADQALPVVGKIPKCIDGVYVRNGANPL 180

Query: 181 HEPMSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGH 240
           +EP++GHH FDGDGMVHAV+F+  G  SYACRFTETQRL QE++ GRPVFPKAIGELHGH
Sbjct: 181 YEPVAGHHFFDGDGMVHAVKFT-NGAASYACRFTETQRLAQEKSLGRPVFPKAIGELHGH 240

Query: 241 SGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKT 300
           SGIARL+LFYAR LF LVD +HG+GVANAGLV+FN  LLAMSEDDLPY +R+T  GDL T
Sbjct: 241 SGIARLLLFYARSLFQLVDGSHGMGVANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTT 300

Query: 301 VGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLP 360
           VGR+DF+GQL STMIAHPKLDPV+G++ ALSYDV+QKPYLKYF+FS +G KSPDVEIPL 
Sbjct: 301 VGRYDFNGQLNSTMIAHPKLDPVNGDLHALSYDVVQKPYLKYFRFSADGVKSPDVEIPLK 360

Query: 361 QPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANSIK 420
           +PTMMHDFAITE FVV+PDQQVVFKL EMI GGSPVVYDK KTSRFGILDKNA DAN+++
Sbjct: 361 EPTMMHDFAITENFVVVPDQQVVFKLTEMITGGSPVVYDKNKTSRFGILDKNAKDANAMR 420

Query: 421 WIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGK 480
           WI+AP+CFCFHLWNAWEEPET+EIVVIGSCMTP DSIFNEC+E+LKSVLSEIRLNL TGK
Sbjct: 421 WIDAPECFCFHLWNAWEEPETDEIVVIGSCMTPADSIFNECDESLKSVLSEIRLNLRTGK 480

Query: 481 STRRPIITETEQVNLEAGMVNRNLLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYL 540
           STRRPII++ EQVNLEAGMVNRN LGRKTQFAYLALAEPWPKVSGFAKVDLF+GE++KY+
Sbjct: 481 STRRPIISDAEQVNLEAGMVNRNKLGRKTQFAYLALAEPWPKVSGFAKVDLFSGEVQKYM 540

Query: 541 YGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRV 594
           YGE+++GGEPLFLP    E E DG+ILAFVHDEKEWKSELQIVNA  L+LEA++KLPSRV
Sbjct: 541 YGEEKFGGEPLFLP--NGEEEGDGYILAFVHDEKEWKSELQIVNAQNLKLEASIKLPSRV 600

BLAST of CaUC07G134270 vs. ExPASy Swiss-Prot
Match: Q9M9F5 (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED9 PE=2 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 1.5e-237
Identity = 413/586 (70.48%), Postives = 480/586 (81.91%), Query Frame = 0

Query: 15  SSSMP--ENLSVNTNYGRNRVATSC----SLHTPSIIQIPKHSHTAFPSPIKTAVPKPLP 74
           SSS P  ++LS ++     ++   C    S++  S +     S T F  P    +   + 
Sbjct: 74  SSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPT-FKPPSWKKLCNDVT 133

Query: 75  VSPPSLSSASEDWNFLQRAAAMALDAVENALVSAE-RKHSLPKTADPAVQIAGNFAPVPE 134
              P  ++ +   N +QR AAM LDAVENA++S E R+H  PKTADPAVQIAGNF PVPE
Sbjct: 134 NLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPE 193

Query: 135 QPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGVSYAC 194
           +PV   LPV G VP+CI+GVYVRNGANPLH+P+SGHHLFDGDGMVHAV F + G VSYAC
Sbjct: 194 KPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF-DNGSVSYAC 253

Query: 195 RFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGL 254
           RFTET RLVQER  GRPVFPKAIGELHGH GIA+LMLF  RGLFGLVD   G+GVANAGL
Sbjct: 254 RFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGL 313

Query: 255 VFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALS 314
           V+FNG LLAMSEDDLPY ++VT  GDL+T GR+DFDGQL STMIAHPK+DP + E+FALS
Sbjct: 314 VYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALS 373

Query: 315 YDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIR 374
           YDV+ KPYLKYF+F+ +GEKSPDVEIPL QPTM+HDFAITE FVVIPDQQVVF+LPEMIR
Sbjct: 374 YDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIR 433

Query: 375 GGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCM 434
           GGSPVVYD++K SRFGIL+KNA DA+SI+WIE PDCFCFHLWN+WEEPET+E+VVIGSCM
Sbjct: 434 GGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGSCM 493

Query: 435 TPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQF 494
           TPPDSIFNE +E L+SVLSEIRLNL TG+STRRP+I  +EQVNLEAGMVNRNLLGRKT++
Sbjct: 494 TPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNRNLLGRKTRY 553

Query: 495 AYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILAFVH 554
           AYLAL EPWPKVSGFAKVDL TGEI+KY+YGE +YGGEPLFLP  G   ED G+I+ FVH
Sbjct: 554 AYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIMVFVH 613

Query: 555 DEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQ 594
           DE++ KSELQ++NA+ ++LEATV LPSRVPYGFHGTFIS EDL KQ
Sbjct: 614 DEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

BLAST of CaUC07G134270 vs. ExPASy TrEMBL
Match: A0A1L5JHV0 (9-cis-epoxycarotenoid dioxygenase NCED1 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 563/598 (94.15%), Postives = 580/598 (96.99%), Query Frame = 0

Query: 1   MASL--SSSSWIKSGFSSSMPENLSVNTNYG-RNRVATSCSLHTPSIIQIPKHSHTAFPS 60
           MASL  SSSSW+KS FSSSMPE +SVN NYG +NRVA  CSLHTPSIIQIPKHS TAF S
Sbjct: 1   MASLGSSSSSWVKSRFSSSMPELVSVNRNYGLQNRVA--CSLHTPSIIQIPKHSQTAFRS 60

Query: 61  PIKTAVPKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQ 120
           P+KTAVPKP+PVSPPS+SS SE+WNFLQRAAA+ALDAVE+ALVSAERKHSLPKTADPAVQ
Sbjct: 61  PVKTAVPKPVPVSPPSVSSGSEEWNFLQRAAAVALDAVEDALVSAERKHSLPKTADPAVQ 120

Query: 121 IAGNFAPVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEF 180
           IAGNFAPVPEQPVRK LPV GKVPDCIRGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEF
Sbjct: 121 IAGNFAPVPEQPVRKALPVTGKVPDCIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEF 180

Query: 181 SEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHN 240
           SEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDH+
Sbjct: 181 SEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHS 240

Query: 241 HGIGVANAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLD 300
           HG+GVANAGLV+FNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQL STMIAHPKLD
Sbjct: 241 HGLGVANAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLD 300

Query: 301 PVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQ 360
           PVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQ
Sbjct: 301 PVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQ 360

Query: 361 VVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPET 420
           VVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDAN+IKWIEAPDCFCFHLWNAWEEPET
Sbjct: 361 VVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANAIKWIEAPDCFCFHLWNAWEEPET 420

Query: 421 NEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVN 480
           +EIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPII ETEQVNLEAGMVN
Sbjct: 421 DEIVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVN 480

Query: 481 RNLLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAE 540
           RN LGRKTQFAYLALAEPWPKVSGFAKVDL TGEI+KYLYGEQRYGGEPLFLPREGA AE
Sbjct: 481 RNQLGRKTQFAYLALAEPWPKVSGFAKVDLATGEIRKYLYGEQRYGGEPLFLPREGATAE 540

Query: 541 DDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 596
           DDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISS+DLQKQIR
Sbjct: 541 DDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSKDLQKQIR 596

BLAST of CaUC07G134270 vs. ExPASy TrEMBL
Match: A0A1S3CQH4 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103503120 PE=3 SV=1)

HSP 1 Score: 1117.8 bits (2890), Expect = 0.0e+00
Identity = 547/592 (92.40%), Postives = 569/592 (96.11%), Query Frame = 0

Query: 4   LSSSSWIKSGFSSSMPENLSVNTNYGRNRVATSCSLHTPSIIQIPKHSHTAFPSPIKTAV 63
           ++SSS++KS FSSSMP+  +++    R  ++ S SLHTPSIIQIPKHSHT FPSP+KT++
Sbjct: 1   MASSSFLKSPFSSSMPDK-TLSNYTSRRLLSVSSSLHTPSIIQIPKHSHTTFPSPLKTSI 60

Query: 64  PKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFA 123
           PKP P+S     S SEDWNFLQRAAAMALDAVENAL+SAERKHSLPKTADPAVQIAGNFA
Sbjct: 61  PKPPPLSTTPPLSGSEDWNFLQRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFA 120

Query: 124 PVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGV 183
           PVPEQPVRKGLPVIGKVP+CIRGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEFSE GGV
Sbjct: 121 PVPEQPVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEAGGV 180

Query: 184 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVA 243
           SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHN GIGVA
Sbjct: 181 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNQGIGVA 240

Query: 244 NAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEM 303
           NAGLV+FNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQL STMIAHPKLDPVSGEM
Sbjct: 241 NAGLVYFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEM 300

Query: 304 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLP 363
           FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVVFKLP
Sbjct: 301 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLP 360

Query: 364 EMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVI 423
           EMIRGGSPVVYDKEKTSRFGILDKNA DAN+IKWIEAPDCFCFHLWNAWEEPETNEIVVI
Sbjct: 361 EMIRGGSPVVYDKEKTSRFGILDKNAADANAIKWIEAPDCFCFHLWNAWEEPETNEIVVI 420

Query: 424 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGR 483
           GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPII+ETEQVNLEAGMVNRNLLGR
Sbjct: 421 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIISETEQVNLEAGMVNRNLLGR 480

Query: 484 KTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHIL 543
           KTQF+YLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHIL
Sbjct: 481 KTQFSYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHIL 540

Query: 544 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 596
           AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR
Sbjct: 541 AFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQIR 591

BLAST of CaUC07G134270 vs. ExPASy TrEMBL
Match: A0A5D3CYC5 (9-cis-epoxycarotenoid dioxygenase NCED3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G001440 PE=3 SV=1)

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 538/571 (94.22%), Postives = 553/571 (96.85%), Query Frame = 0

Query: 26  TNY-GRNRVATSCSLHTPSIIQIPKHSHTAFPSPIKTAVPKPLPVSPPSLSSASEDWNFL 85
           +NY  R  ++ S SLHTPSIIQIPKHSHT FPSP+KT++PKP P+S     S SEDWNFL
Sbjct: 7   SNYTSRRLLSVSSSLHTPSIIQIPKHSHTTFPSPLKTSIPKPPPLSTTPPLSGSEDWNFL 66

Query: 86  QRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVPDCI 145
           QRAAAMALDAVENAL+SAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVP+CI
Sbjct: 67  QRAAAMALDAVENALISAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVPECI 126

Query: 146 RGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAYGRP 205
           RGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEFSE GGVSYACRFTETQRLVQERAYGRP
Sbjct: 127 RGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFSEAGGVSYACRFTETQRLVQERAYGRP 186

Query: 206 VFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAMSEDDLPY 265
           VFPKAIGELHGHSGIARLMLFYARGLFGLVDHN GIGVANAGLV+FNGRLLAMSEDDLPY
Sbjct: 187 VFPKAIGELHGHSGIARLMLFYARGLFGLVDHNQGIGVANAGLVYFNGRLLAMSEDDLPY 246

Query: 266 QIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPE 325
           QIRVTPAGDLKTVGRFDFDGQL STMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPE
Sbjct: 247 QIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPE 306

Query: 326 GEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGI 385
           GEKSPDVEIPLPQPTMMHDFAITEK+VVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGI
Sbjct: 307 GEKSPDVEIPLPQPTMMHDFAITEKYVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGI 366

Query: 386 LDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSV 445
           LDKNA DAN+IKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSV
Sbjct: 367 LDKNAADANAIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSV 426

Query: 446 LSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQFAYLALAEPWPKVSGFAK 505
           LSEIRLNLSTGKSTRRPII+ETEQVNLEAGMVNRNLLGRKTQF+YLALAEPWPKVSGFAK
Sbjct: 427 LSEIRLNLSTGKSTRRPIISETEQVNLEAGMVNRNLLGRKTQFSYLALAEPWPKVSGFAK 486

Query: 506 VDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAMTL 565
           VDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAMTL
Sbjct: 487 VDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILAFVHDEKEWKSELQIVNAMTL 546

Query: 566 ELEATVKLPSRVPYGFHGTFISSEDLQKQIR 596
           ELEATVKLPSRVPYGFHGTFISSEDLQKQIR
Sbjct: 547 ELEATVKLPSRVPYGFHGTFISSEDLQKQIR 577

BLAST of CaUC07G134270 vs. ExPASy TrEMBL
Match: A0A6J1ETU1 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111437497 PE=3 SV=1)

HSP 1 Score: 1097.0 bits (2836), Expect = 0.0e+00
Identity = 534/593 (90.05%), Postives = 563/593 (94.94%), Query Frame = 0

Query: 1   MASLSSSSWIKSGFSSSMPENLSVNTNYGRNRVATSCSLHTPSIIQIPKHSHTAFPSPIK 60
           + S SSSSW+KSGFSSSM E  SVN + GRNRVA  CSLHTPSIIQIPK S TAF SP+K
Sbjct: 4   LVSSSSSSWVKSGFSSSMSECFSVNMDSGRNRVA--CSLHTPSIIQIPKRSQTAFRSPVK 63

Query: 61  TAVPKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAG 120
           T VPKP+PVS PS+   SE+WNFLQRAAAMALDAVE+ALVSAE+KHSLPKTADP+VQIAG
Sbjct: 64  TTVPKPVPVSAPSV---SEEWNFLQRAAAMALDAVEDALVSAEKKHSLPKTADPSVQIAG 123

Query: 121 NFAPVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEG 180
           NFAPVPEQ VRKGLPVIGKVP+CIRGVYVRNGANPLHEP+SGHHLFDGDGMVHAVEF+E 
Sbjct: 124 NFAPVPEQSVRKGLPVIGKVPECIRGVYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTED 183

Query: 181 GGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGI 240
           GGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HGI
Sbjct: 184 GGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGI 243

Query: 241 GVANAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVS 300
           GVANAGLV+FNG+LLAMSEDDLPY+IRVTPAGDLKTVGRFDFDGQL STMIAHPKLDPVS
Sbjct: 244 GVANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVS 303

Query: 301 GEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVF 360
           GEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFV+IPDQQVVF
Sbjct: 304 GEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVF 363

Query: 361 KLPEMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEI 420
           KLPEMIRGGSPV+YDKEKTSRFG++DKNATDA++IKWI+ PDCFCFHLWNAWEEPET EI
Sbjct: 364 KLPEMIRGGSPVIYDKEKTSRFGVMDKNATDADAIKWIDVPDCFCFHLWNAWEEPETEEI 423

Query: 421 VVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNL 480
           VVIGSCMTPPDSIFNECEENL S+LSEIRLNLSTGKSTRRPII ETEQVNLEAGMVNRN 
Sbjct: 424 VVIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNQ 483

Query: 481 LGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDG 540
           LGRKTQF Y+ALAEPWPKVSGF KV+LFTGE +KYLYGEQRYGGEPLFLPREGAEAEDDG
Sbjct: 484 LGRKTQFVYVALAEPWPKVSGFVKVNLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDDG 543

Query: 541 HILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQ 594
           HIL FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISS DLQKQ
Sbjct: 544 HILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 591

BLAST of CaUC07G134270 vs. ExPASy TrEMBL
Match: A0A6J1JCI7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111483246 PE=3 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 532/592 (89.86%), Postives = 559/592 (94.43%), Query Frame = 0

Query: 2   ASLSSSSWIKSGFSSSMPENLSVNTNYGRNRVATSCSLHTPSIIQIPKHSHTAFPSPIKT 61
           +S SSSSW+KSGFSS M E  SVN + GRNRVA  CSLHTPSIIQIPK S TAF SP+KT
Sbjct: 6   SSSSSSSWVKSGFSSLMSERFSVNMDSGRNRVA--CSLHTPSIIQIPKRSQTAFRSPVKT 65

Query: 62  AVPKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGN 121
            VPKP+PV  PS+   SE+WNFLQRAAAMALDAVE+ALVS ERKHSLPKTADP+VQIAGN
Sbjct: 66  TVPKPVPVPAPSV---SEEWNFLQRAAAMALDAVEDALVSVERKHSLPKTADPSVQIAGN 125

Query: 122 FAPVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGG 181
           FAPVPEQPVRKGLPVIGKVP+ IRG+YVRNGANPLHEP+SGHHLFDGDGMVHAVEF+E G
Sbjct: 126 FAPVPEQPVRKGLPVIGKVPESIRGIYVRNGANPLHEPVSGHHLFDGDGMVHAVEFTEDG 185

Query: 182 GVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIG 241
            VSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARL+LFYARGLFGLVDH+HGIG
Sbjct: 186 EVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLLLFYARGLFGLVDHSHGIG 245

Query: 242 VANAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSG 301
           VANAGLV+FNG+LLAMSEDDLPY+IRVTPAGDLKTVGRFDFDGQL STMIAHPKLDPVSG
Sbjct: 246 VANAGLVYFNGQLLAMSEDDLPYEIRVTPAGDLKTVGRFDFDGQLKSTMIAHPKLDPVSG 305

Query: 302 EMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFK 361
           EMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFV+IPDQQVVFK
Sbjct: 306 EMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVIIPDQQVVFK 365

Query: 362 LPEMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIV 421
           LPEMIRGGSPV+YDKEKTSRFG++DKNATDA +IKWI+ PDCFCFHLWNAWEEPET EIV
Sbjct: 366 LPEMIRGGSPVIYDKEKTSRFGVMDKNATDATAIKWIDVPDCFCFHLWNAWEEPETEEIV 425

Query: 422 VIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLL 481
           VIGSCMTPPDSIFNECEENL S+LSEIRLNLSTGKSTRRPII ETEQVNLEAGMVNRN L
Sbjct: 426 VIGSCMTPPDSIFNECEENLNSILSEIRLNLSTGKSTRRPIIAETEQVNLEAGMVNRNRL 485

Query: 482 GRKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGH 541
           GRKTQFAY+ALAEPWPKVSGF KVDLFTGE +KYLYGEQRYGGEPLFLPREGAEAEDDGH
Sbjct: 486 GRKTQFAYVALAEPWPKVSGFVKVDLFTGESRKYLYGEQRYGGEPLFLPREGAEAEDDGH 545

Query: 542 ILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQ 594
           IL FVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISS DLQKQ
Sbjct: 546 ILTFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSNDLQKQ 592

BLAST of CaUC07G134270 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 879.8 bits (2272), Expect = 1.2e-255
Identity = 435/592 (73.48%), Postives = 492/592 (83.11%), Query Frame = 0

Query: 4   LSSSSWIKSGFSSSMPENLSVNTNYGRNRVATSCSLHTPSIIQIPKHSHTAFPSPIKTAV 63
           LSSS      + SS+P    V       ++  S +LHTP  +  PK S  +         
Sbjct: 24  LSSSQSSDLSYCSSLPMASRVT-----RKLNVSSALHTPPALHFPKQSSNS--------- 83

Query: 64  PKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFA 123
              + V P +  S ++  N  QRAAA ALDA E  LVS E+ H LPKTADP+VQIAGNFA
Sbjct: 84  -PAIVVKPKAKESNTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFA 143

Query: 124 PVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGV 183
           PV EQPVR+ LPV+GK+PD I+GVYVRNGANPLHEP++GHH FDGDGMVHAV+F E G  
Sbjct: 144 PVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKF-EHGSA 203

Query: 184 SYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVA 243
           SYACRFT+T R VQER  GRPVFPKAIGELHGH+GIARLMLFYAR   G+VD  HG GVA
Sbjct: 204 SYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLMLFYARAAAGIVDPAHGTGVA 263

Query: 244 NAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEM 303
           NAGLV+FNGRLLAMSEDDLPYQ+++TP GDLKTVGRFDFDGQL STMIAHPK+DP SGE+
Sbjct: 264 NAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGEL 323

Query: 304 FALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLP 363
           FALSYDV+ KPYLKYF+FSP+G KSPDVEI L QPTMMHDFAITE FVV+PDQQVVFKLP
Sbjct: 324 FALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLP 383

Query: 364 EMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVI 423
           EMIRGGSPVVYDK K +RFGILDK A D+++IKWI+APDCFCFHLWNAWEEPET+E+VVI
Sbjct: 384 EMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVVI 443

Query: 424 GSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIIT-ETEQVNLEAGMVNRNLLG 483
           GSCMTPPDSIFNE +ENLKSVLSEIRLNL TG+STRRPII+ E +QVNLEAGMVNRN+LG
Sbjct: 444 GSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLG 503

Query: 484 RKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHI 543
           RKT+FAYLALAEPWPKVSGFAKVDL TGE+KK+LYG+ RYGGEPLFLP EG E ED+G+I
Sbjct: 504 RKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRYGGEPLFLPGEGGE-EDEGYI 563

Query: 544 LAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQI 595
           L FVHDEK WKSELQIVNA++LE+EATVKLPSRVPYGFHGTFI ++DL KQ+
Sbjct: 564 LCFVHDEKTWKSELQIVNAVSLEVEATVKLPSRVPYGFHGTFIGADDLAKQV 598

BLAST of CaUC07G134270 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 823.5 bits (2126), Expect = 1.1e-238
Identity = 413/586 (70.48%), Postives = 480/586 (81.91%), Query Frame = 0

Query: 15  SSSMP--ENLSVNTNYGRNRVATSC----SLHTPSIIQIPKHSHTAFPSPIKTAVPKPLP 74
           SSS P  ++LS ++     ++   C    S++  S +     S T F  P    +   + 
Sbjct: 74  SSSRPKLQSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPT-FKPPSWKKLCNDVT 133

Query: 75  VSPPSLSSASEDWNFLQRAAAMALDAVENALVSAE-RKHSLPKTADPAVQIAGNFAPVPE 134
              P  ++ +   N +QR AAM LDAVENA++S E R+H  PKTADPAVQIAGNF PVPE
Sbjct: 134 NLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPVPE 193

Query: 135 QPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGVSYAC 194
           +PV   LPV G VP+CI+GVYVRNGANPLH+P+SGHHLFDGDGMVHAV F + G VSYAC
Sbjct: 194 KPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRF-DNGSVSYAC 253

Query: 195 RFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGL 254
           RFTET RLVQER  GRPVFPKAIGELHGH GIA+LMLF  RGLFGLVD   G+GVANAGL
Sbjct: 254 RFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGL 313

Query: 255 VFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALS 314
           V+FNG LLAMSEDDLPY ++VT  GDL+T GR+DFDGQL STMIAHPK+DP + E+FALS
Sbjct: 314 VYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALS 373

Query: 315 YDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIR 374
           YDV+ KPYLKYF+F+ +GEKSPDVEIPL QPTM+HDFAITE FVVIPDQQVVF+LPEMIR
Sbjct: 374 YDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIR 433

Query: 375 GGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCM 434
           GGSPVVYD++K SRFGIL+KNA DA+SI+WIE PDCFCFHLWN+WEEPET+E+VVIGSCM
Sbjct: 434 GGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIGSCM 493

Query: 435 TPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQF 494
           TPPDSIFNE +E L+SVLSEIRLNL TG+STRRP+I  +EQVNLEAGMVNRNLLGRKT++
Sbjct: 494 TPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVI--SEQVNLEAGMVNRNLLGRKTRY 553

Query: 495 AYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFLPREGAEAEDDGHILAFVH 554
           AYLAL EPWPKVSGFAKVDL TGEI+KY+YGE +YGGEPLFLP  G   ED G+I+ FVH
Sbjct: 554 AYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP-SGDGEEDGGYIMVFVH 613

Query: 555 DEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQ 594
           DE++ KSELQ++NA+ ++LEATV LPSRVPYGFHGTFIS EDL KQ
Sbjct: 614 DEEKVKSELQLINAVNMKLEATVTLPSRVPYGFHGTFISKEDLSKQ 654

BLAST of CaUC07G134270 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 811.6 bits (2095), Expect = 4.2e-235
Identity = 394/570 (69.12%), Postives = 462/570 (81.05%), Query Frame = 0

Query: 25  NTNYGRNRVATSCSLHTPSIIQIPKHSHTAFPSPIKTAVPKPLPVSPPSLSSASEDWNFL 84
           NT   R +++ +    TP+++  P +             P P P+ P   +S    WN L
Sbjct: 36  NTKPRRRKLSANSVSDTPNLLNFPNY-------------PSPNPIIPEKDTSR---WNPL 95

Query: 85  QRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVPDCI 144
           QRAA+ ALD  E AL+  ER   LPKT DP  QI+GN+APVPEQ V+  L V GK+PDCI
Sbjct: 96  QRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCI 155

Query: 145 RGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAYGRP 204
            GVY+RNGANPL EP+SGHHLFDGDGMVHAV+ +  G  SY+CRFTET+RLVQE+  G P
Sbjct: 156 DGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKIT-NGDASYSCRFTETERLVQEKQLGSP 215

Query: 205 VFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAMSEDDLPY 264
           +FPKAIGELHGHSGIARLMLFYARGLFGL++H +G GVANAGLV+F+ RLLAMSEDDLPY
Sbjct: 216 IFPKAIGELHGHSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPY 275

Query: 265 QIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKFSPE 324
           Q+RVT  GDL+T+GRFDFDGQL+S MIAHPK+DPV+ E+FALSYDV++KPYLKYFKFSPE
Sbjct: 276 QVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPE 335

Query: 325 GEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSRFGI 384
           GEKSPDVEIPL  PTMMHDFAITE FVVIPDQQVVFKL +M  G SPV YD EK SRFGI
Sbjct: 336 GEKSPDVEIPLASPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGI 395

Query: 385 LDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENLKSV 444
           L +NA DA+ + W+E+P+ FCFHLWNAWE PET+E+VVIGSCMTP DSIFNEC+E L SV
Sbjct: 396 LPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSV 455

Query: 445 LSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQFAYLALAEPWPKVSGFAK 504
           LSEIRLNL TGKSTRR II  + Q+NLEAGMVNRNLLGRKT++AYLA+AEPWPKVSGFAK
Sbjct: 456 LSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAK 515

Query: 505 VDLFTGEIKKYLYGEQRYGGEPLFLPRE-GAEAEDDGHILAFVHDEKEWKSELQIVNAMT 564
           VDL TGE+K + YG ++YGGEP FLPR   ++ EDDG+I++FVHDE+ W+SEL IVNA+T
Sbjct: 516 VDLSTGEVKNHFYGGKKYGGEPFFLPRGLESDGEDDGYIMSFVHDEESWESELHIVNAVT 575

Query: 565 LELEATVKLPSRVPYGFHGTFISSEDLQKQ 594
           LELEATVKLPSRVPYGFHGTF++S D+  Q
Sbjct: 576 LELEATVKLPSRVPYGFHGTFVNSADMLNQ 588

BLAST of CaUC07G134270 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 761.5 bits (1965), Expect = 4.9e-220
Identity = 363/516 (70.35%), Postives = 432/516 (83.72%), Query Frame = 0

Query: 82  NFLQRAAAMALDAVENALVSAERKHSLPKTADPAVQIAGNFAPVPEQPVRKGLPVIGKVP 141
           N  Q+AAA+A+DA E AL+S E+   LPKTADP VQIAGN++PVPE  VR+ L V G +P
Sbjct: 70  NIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGTIP 129

Query: 142 DCIRGVYVRNGANPLHEPMSGHHLFDGDGMVHAVEFSEGGGVSYACRFTETQRLVQERAY 201
           DCI GVY+RNGANP+ EP +GHHLFDGDGMVHAV+ +  G  SYACRFT+T+RLVQE+  
Sbjct: 130 DCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKIT-NGSASYACRFTKTERLVQEKRL 189

Query: 202 GRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHNHGIGVANAGLVFFNGRLLAMSEDD 261
           GRPVFPKAIGELHGHSGIARLMLFYARGL GL+++ +G+GVANAGLV+FN RLLAMSEDD
Sbjct: 190 GRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSEDD 249

Query: 262 LPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAHPKLDPVSGEMFALSYDVIQKPYLKYFKF 321
           LPYQ+++T  GDL+TVGR+DFDGQL S MIAHPKLDPV+ E+ ALSYDV++KPYLKYF+F
Sbjct: 250 LPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFRF 309

Query: 322 SPEGEKSPDVEIPLPQPTMMHDFAITEKFVVIPDQQVVFKLPEMIRGGSPVVYDKEKTSR 381
           SP+G KSP++EIPL  PTM+HDFAITE FVVIPDQQVVFKL EMI G SPVV+D EK SR
Sbjct: 310 SPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGEKVSR 369

Query: 382 FGILDKNATDANSIKWIEAPDCFCFHLWNAWEEPETNEIVVIGSCMTPPDSIFNECEENL 441
            GI+ K+AT+A+ I W+ +P+ FCFHLWNAWE PET EIVVIGSCM+P DSIFNE +E+L
Sbjct: 370 LGIMPKDATEASQIIWVNSPETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDESL 429

Query: 442 KSVLSEIRLNLSTGKSTRRPIITETEQVNLEAGMVNRNLLGRKTQFAYLALAEPWPKVSG 501
           +SVLSEIR+NL T K+TRR ++   E VNLE GMVNRN LGRKT+FA+LA+A PWPKVSG
Sbjct: 430 RSVLSEIRINLRTRKTTRRSLLV-NEDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKVSG 489

Query: 502 FAKVDLFTGEIKKYLYGEQRYGGEPLFLP---REGAEAEDDGHILAFVHDEKEWKSELQI 561
           FAKVDL TGE+KKY+YG ++YGGEP FLP     G E EDDG+I   VHDE+   SELQI
Sbjct: 490 FAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYIFCHVHDEETKTSELQI 549

Query: 562 VNAMTLELEATVKLPSRVPYGFHGTFISSEDLQKQI 595
           +NA+ L+LEAT+KLPSRVPYGFHGTF+ S +L  Q+
Sbjct: 550 INAVNLKLEATIKLPSRVPYGFHGTFVDSNELVDQL 583

BLAST of CaUC07G134270 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 651.4 bits (1679), Expect = 7.2e-187
Identity = 314/545 (57.61%), Postives = 407/545 (74.68%), Query Frame = 0

Query: 55  FPSPIKTAVPKPLPVSPPSLSSASEDWNFLQRAAAMALDAVENALV-SAERKHSLPKTAD 114
           F  P    +  P+P SP  L     + N LQ+ AA  LD +E+++V   E+   LPK  D
Sbjct: 38  FKIPTLPDLTSPVP-SPVKLKPTYPNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTD 97

Query: 115 PAVQIAGNFAPVPEQPVRKGLPVIGKVPDCIRGVYVRNGANPLHEPMSGHHLFDGDGMVH 174
           PAVQ++GNFAPV E PV+ GL V+G++P C++GVY+RNGANP+  P++GHHLFDGDGM+H
Sbjct: 98  PAVQLSGNFAPVNECPVQNGLEVVGQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIH 157

Query: 175 AVEFSEGGGVSYACRFTETQRLVQERAYGRPVFPKAIGELHGHSGIARLMLFYARGLFGL 234
           AV       VSY+CR+T+T RLVQE A GR VFPK IGELHGHSG+ARL LF AR   GL
Sbjct: 158 AVSIGFDNQVSYSCRYTKTNRLVQETALGRSVFPKPIGELHGHSGLARLALFTARAGIGL 217

Query: 235 VDHNHGIGVANAGLVFFNGRLLAMSEDDLPYQIRVTPAGDLKTVGRFDFDGQLTSTMIAH 294
           VD   G+GVANAG+VFFNGRLLAMSEDDLPYQ+++   GDL+T+GRF FD Q+ S++IAH
Sbjct: 218 VDGTRGMGVANAGVVFFNGRLLAMSEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAH 277

Query: 295 PKLDPVSGEMFALSYDVIQKPYLKYFKFSPEGEKSPDVEIPLPQPTMMHDFAITEKFVVI 354
           PK+D  +G++  LSY+V++KP+L+Y KF+  G+K+ DVEI LP+PTM+HDFAITE FVVI
Sbjct: 278 PKVDATTGDLHTLSYNVLKKPHLRYLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVI 337

Query: 355 PDQQVVFKLPEMIRGGSPVVYDKEKTSRFGILDKNATDANSIKWIEAPDCFCFHLWNAWE 414
           PDQQ+VFKL EMIRGGSPV+Y KEK +RFG+L K     + I W++ PDCFCFHLWNAWE
Sbjct: 338 PDQQMVFKLSEMIRGGSPVIYVKEKMARFGVLSKQDLTGSDINWVDVPDCFCFHLWNAWE 397

Query: 415 EPETNE----IVVIGSCMTPPDSIFNECEENLKSVLSEIRLNLSTGKSTRRPIITETEQV 474
           E  T E    IVVIGSCM+PPD+IF+E  E  +  LSEIRLN+ T +S R+ I+T    V
Sbjct: 398 E-RTEEGDPVIVVIGSCMSPPDTIFSESGEPTRVELSEIRLNMRTKESNRKVIVT---GV 457

Query: 475 NLEAGMVNRNLLGRKTQFAYLALAEPWPKVSGFAKVDLFTGEIKKYLYGEQRYGGEPLFL 534
           NLEAG +NR+ +GRK+QF Y+A+A+PWPK SG AKVD+  G + ++ YG  R+GGEP F+
Sbjct: 458 NLEAGHINRSYVGRKSQFVYIAIADPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCFV 517

Query: 535 PREGAEAEDDGHILAFVHDEKEWKSELQIVNAMTLELEATVKLPSRVPYGFHGTFISSED 594
           P EG   ED G+++ FV DE++ +SE  +V+A  ++  A V+LP RVPYGFHGTF+S   
Sbjct: 518 P-EGEGEEDKGYVMGFVRDEEKDESEFVVVDATDMKQVAAVRLPERVPYGFHGTFVSENQ 576

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
APO14273.10.0e+0094.159-cis-epoxycarotenoid dioxygenase NCED1 [Luffa aegyptiaca][more]
XP_038878047.10.0e+0093.779-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic [Benincasa hispida][more]
XP_008465499.10.0e+0092.40PREDICTED: 9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like [Cucumis ... [more]
XP_004147768.10.0e+0091.449-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic [Cucumis sativus] >KAE864... [more]
KAA0062986.10.0e+0094.229-cis-epoxycarotenoid dioxygenase NCED3 [Cucumis melo var. makuwa] >TYK16360.1 9... [more]
Match NameE-valueIdentityDescription
Q9LRR71.8e-25473.489-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
O240231.5e-25371.719-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O... [more]
K4CJJ13.4e-25074.039-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O... [more]
Q9M6E85.8e-25070.349-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=... [more]
Q9M9F51.5e-23770.489-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A1L5JHV00.0e+0094.159-cis-epoxycarotenoid dioxygenase NCED1 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1[more]
A0A1S3CQH40.0e+0092.409-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucumis melo OX=3... [more]
A0A5D3CYC50.0e+0094.229-cis-epoxycarotenoid dioxygenase NCED3 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A6J1ETU10.0e+0090.059-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita moschat... [more]
A0A6J1JCI70.0e+0089.869-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic-like OS=Cucurbita maxima ... [more]
Match NameE-valueIdentityDescription
AT3G14440.11.2e-25573.48nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT1G78390.11.1e-23870.48nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT1G30100.14.2e-23569.12nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT4G18350.14.9e-22070.35nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT3G24220.17.2e-18757.61nine-cis-epoxycarotenoid dioxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 123..585
e-value: 2.9E-116
score: 389.4
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 65..593
NoneNo IPR availablePANTHERPTHR10543:SF1239-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATEDcoord: 65..593

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC07G134270.1CaUC07G134270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
biological_process GO:0010162 seed dormancy process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0045549 9-cis-epoxycarotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen