CaUC07G127770 (gene) Watermelon (USVL246-FR2) v1

BLAST of CaUC07G127770 vs. NCBI nr
Match: XP_008455316.1 (PREDICTED: two pore calcium channel protein 1B isoform X1 [Cucumis melo])

HSP 1 Score: 1369.0 bits (3542), Expect = 0.0e+00
Identity = 702/733 (95.77%), Postives = 716/733 (97.68%), Query Frame = 0

Query: 1   MEESLL---GGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIP 60
           MEESLL   GGGGESS SS+SRRQ PHVRFRRRSDAIAYGSAYQRAAALVDLAEDG+GIP
Sbjct: 1   MEESLLGGGGGGGESSGSSTSRRQTPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGVGIP 60

Query: 61  EKILDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFF 120
           EKILDQSNFQSAAKLYFIFIKFD IWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFF
Sbjct: 61  EKILDQSNFQSAAKLYFIFIKFDWIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFF 120

Query: 121 LGQLPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFL 180
           LGQLPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYW N+LNQLKVICI ILVADFL
Sbjct: 121 LGQLPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWTNLLNQLKVICIIILVADFL 180

Query: 181 VYTLYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLL 240
           VYTLYLSPVAFDYLP R APYIRV+FFIMNIRHLRESIIILAGMLGTYLNV+ALW LFLL
Sbjct: 181 VYTLYLSPVAFDYLPFRAAPYIRVIFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLL 240

Query: 241 FSSWLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLY 300
           FSSWLAYVIFEDTQQGKLIFTSFG TLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLY
Sbjct: 241 FSSWLAYVIFEDTQQGKLIFTSFGTTLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLY 300

Query: 301 VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCL 360
           VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCL
Sbjct: 301 VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCL 360

Query: 361 ILLEELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWF 420
           +LLEELNRYRTLPKISRDDYELIFDELDDSRDFKIN+NEFDDLCNAIALKFQKEDIPSWF
Sbjct: 361 MLLEELNRYRTLPKISRDDYELIFDELDDSRDFKININEFDDLCNAIALKFQKEDIPSWF 420

Query: 421 EGYPSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELE 480
           EGYPSIYHS FSKKLKAFVRSP FGYAVSFILILNL+AVIIETTLDIENNVGQKAWQELE
Sbjct: 421 EGYPSIYHSSFSKKLKAFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELE 480

Query: 481 FVFGWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGE 540
           FVFGWIYVVEMALKIYA+GF+NYWRDGQNQFDFL+TWIIVIGETITFVAPSGLTFLSNGE
Sbjct: 481 FVFGWIYVVEMALKIYAYGFMNYWRDGQNQFDFLITWIIVIGETITFVAPSGLTFLSNGE 540

Query: 541 WIRYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG 600
           WIRYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG
Sbjct: 541 WIRYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG 600

Query: 601 GIVNAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS 660
           GIVNAGNPTL KTDL+DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS
Sbjct: 601 GIVNAGNPTLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS 660

Query: 661 LVYFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKS 720
           LVYFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQD DSRKDR RFVGTK+
Sbjct: 661 LVYFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDSDSRKDRPRFVGTKT 720

Query: 721 RSRKVDILLHHML 731
           RSRKVDILLHHML
Sbjct: 721 RSRKVDILLHHML 733

BLAST of CaUC07G127770 vs. NCBI nr
Match: XP_031744917.1 (two pore calcium channel protein 1A [Cucumis sativus] >KGN43617.1 hypothetical protein Csa_020586 [Cucumis sativus])

HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 699/730 (95.75%), Postives = 716/730 (98.08%), Query Frame = 0

Query: 1   MEESLLGGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60
           MEESLLGGGGESS SS+SRR  PHVRFRRRSDAIAYGSAYQRAAALVDLAEDG+GIPEKI
Sbjct: 1   MEESLLGGGGESSGSSTSRR--PHVRFRRRSDAIAYGSAYQRAAALVDLAEDGVGIPEKI 60

Query: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120
           LDQSNFQSAAKLYFIFIKFD IWSLNFFALIVLNFFEKPLWC KYDTHSCNDREYFFLGQ
Sbjct: 61  LDQSNFQSAAKLYFIFIKFDWIWSLNFFALIVLNFFEKPLWCFKYDTHSCNDREYFFLGQ 120

Query: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYT 180
           LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYW N+LNQLKVICITILVADFLVYT
Sbjct: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWTNLLNQLKVICITILVADFLVYT 180

Query: 181 LYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSS 240
           LYLSPVAFDYLP R APYIRVVFFIMNIRHLRESIIILAGMLGTYLNV+ALW LFLLFSS
Sbjct: 181 LYLSPVAFDYLPFRAAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSS 240

Query: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300
           WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLL
Sbjct: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLL 300

Query: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILL 360
           GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVG+INKEQCL+LL
Sbjct: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGYINKEQCLMLL 360

Query: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420
           EELNRYR+LPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY
Sbjct: 361 EELNRYRSLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420

Query: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVF 480
           PS+YHS  SKKLKAFVRSP FGYAVSFIL+LNL+AVIIETTLDIENNVGQKAWQELEFVF
Sbjct: 421 PSVYHSGSSKKLKAFVRSPKFGYAVSFILVLNLVAVIIETTLDIENNVGQKAWQELEFVF 480

Query: 481 GWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540
           GWIYVVEMALKIYA+GF+NYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR
Sbjct: 481 GWIYVVEMALKIYAYGFMNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540

Query: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600
           YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV
Sbjct: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600

Query: 601 NAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVY 660
           NAGNPTLSKT+L+DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSY+ELTGSIWSLVY
Sbjct: 601 NAGNPTLSKTELDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYRELTGSIWSLVY 660

Query: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSR 720
           FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQD DSRKDR RFVGTK+RSR
Sbjct: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDKDSRKDRPRFVGTKTRSR 720

Query: 721 KVDILLHHML 731
           KVDILLHHML
Sbjct: 721 KVDILLHHML 728

BLAST of CaUC07G127770 vs. NCBI nr
Match: XP_038887758.1 (two pore calcium channel protein 1A [Benincasa hispida])

HSP 1 Score: 1353.2 bits (3501), Expect = 0.0e+00
Identity = 699/731 (95.62%), Postives = 718/731 (98.22%), Query Frame = 0

Query: 1   MEESLLGGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60
           MEESLL GGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI
Sbjct: 1   MEESLLVGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60

Query: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120
           LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKY+THSCNDREYFFLGQ
Sbjct: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYNTHSCNDREYFFLGQ 120

Query: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYT 180
           LPYLTAVESLIYEAVTLIIL+IHTFFPISYEGAQLYWKN+LNQLKVICI ILVADFLVYT
Sbjct: 121 LPYLTAVESLIYEAVTLIILIIHTFFPISYEGAQLYWKNLLNQLKVICIIILVADFLVYT 180

Query: 181 LYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSS 240
           ++LSPVAFDYLP RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNV+ALWLLFLLFSS
Sbjct: 181 VFLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWLLFLLFSS 240

Query: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300
           WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL
Sbjct: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300

Query: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILL 360
           GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDT+NVGFINKEQCLILL
Sbjct: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTDNVGFINKEQCLILL 360

Query: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420
           EELNRYRTLPKISRDDYELIFDELDDSRDFKINM+EFDDLCNAIALKFQKEDIPSWFEGY
Sbjct: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMDEFDDLCNAIALKFQKEDIPSWFEGY 420

Query: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFV- 480
           PS+YHSVFSKKLKAFVRSPTFGYAVSFILILNL+AVIIETTLDIENN+G    Q + F+ 
Sbjct: 421 PSVYHSVFSKKLKAFVRSPTFGYAVSFILILNLVAVIIETTLDIENNMG----QSIPFLP 480

Query: 481 FGWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWI 540
            GWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWI
Sbjct: 481 TGWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWI 540

Query: 541 RYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGI 600
           RYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLG+QVFGGI
Sbjct: 541 RYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGMQVFGGI 600

Query: 601 VNAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLV 660
           VNAGNPTLSKTDL+DDDYLLFNFND+PNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLV
Sbjct: 601 VNAGNPTLSKTDLDDDDYLLFNFNDFPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLV 660

Query: 661 YFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRS 720
           YFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGE+QDQDGDS+KDRRR VGTK+RS
Sbjct: 661 YFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEQQDQDGDSKKDRRRLVGTKTRS 720

Query: 721 RKVDILLHHML 731
            KVDILLHHML
Sbjct: 721 SKVDILLHHML 727

BLAST of CaUC07G127770 vs. NCBI nr
Match: XP_022930707.1 (two pore calcium channel protein 1A-like [Cucurbita moschata])

HSP 1 Score: 1348.2 bits (3488), Expect = 0.0e+00
Identity = 692/730 (94.79%), Postives = 716/730 (98.08%), Query Frame = 0

Query: 1   MEESLLGGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60
           M+ESLL  G ESSSSSSSRRQIPHV+F+RRSDAIAYGSAYQRAAALVDLAEDG+GIPEKI
Sbjct: 1   MDESLL--GVESSSSSSSRRQIPHVKFKRRSDAIAYGSAYQRAAALVDLAEDGVGIPEKI 60

Query: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120
           LDQSNFQSAAKLYFIFIKFDLIWS+NFFAL+VLNFFEKPLWCSKYDTHSCNDREYFFLGQ
Sbjct: 61  LDQSNFQSAAKLYFIFIKFDLIWSVNFFALVVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120

Query: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYT 180
           LPYLTAVESLIYEAVTLIILMIHTFFPIS+EGAQLYWKN LNQLKVIC+ ILVADFL+YT
Sbjct: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISFEGAQLYWKNPLNQLKVICLIILVADFLIYT 180

Query: 181 LYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSS 240
           LYLSPVAFDYLP RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNV+ALW LFLLFSS
Sbjct: 181 LYLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSS 240

Query: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300
           WLAYVIFEDTQQGKL+FTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLL
Sbjct: 241 WLAYVIFEDTQQGKLLFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLL 300

Query: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILL 360
           GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDT+NVGFINK+QCLILL
Sbjct: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTDNVGFINKQQCLILL 360

Query: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420
           EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEF DLCNAIALKFQKEDIPS FE Y
Sbjct: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSVFEAY 420

Query: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVF 480
           PSIY+SV SKKLKAFVRSP+FGYA+SFILILNL+AVIIETTLDIENNVGQKAWQELEFVF
Sbjct: 421 PSIYNSVSSKKLKAFVRSPSFGYAISFILILNLVAVIIETTLDIENNVGQKAWQELEFVF 480

Query: 481 GWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540
           GWIYVVEMALKIYAFGF+NYWRDGQNQFDFLVTWIIVIGET+TFVAPSGLTFLSNGEWIR
Sbjct: 481 GWIYVVEMALKIYAFGFMNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIR 540

Query: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600
           YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+V
Sbjct: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLV 600

Query: 601 NAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVY 660
           NAGNP LSKTDL+DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS VY
Sbjct: 601 NAGNPALSKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSHVY 660

Query: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSR 720
           FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQ+QD DSRKDRRRFVGTK+RSR
Sbjct: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQEQDADSRKDRRRFVGTKTRSR 720

Query: 721 KVDILLHHML 731
           KVDILLHHML
Sbjct: 721 KVDILLHHML 728

BLAST of CaUC07G127770 vs. NCBI nr
Match: XP_022968991.1 (two pore calcium channel protein 1A-like [Cucurbita maxima])

HSP 1 Score: 1346.3 bits (3483), Expect = 0.0e+00
Identity = 689/730 (94.38%), Postives = 711/730 (97.40%), Query Frame = 0

Query: 1   MEESLLGGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60
           MEESLL  GGE SSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI
Sbjct: 1   MEESLL--GGEGSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60

Query: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120
           LDQSNFQSAA+LYFIFI+FD +WSLNFFALI+LNFFE+PLWCSKY+THSCNDREYFFLGQ
Sbjct: 61  LDQSNFQSAARLYFIFIRFDFLWSLNFFALIILNFFEEPLWCSKYETHSCNDREYFFLGQ 120

Query: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYT 180
           LPYLTAVESLIYEA TLIIL IHTFFPISYEGAQLYWKN LNQLKV+C  ILVADF++YT
Sbjct: 121 LPYLTAVESLIYEATTLIILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYT 180

Query: 181 LYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSS 240
           LYLSPVAFDYLP RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNV+AL LLFLLFSS
Sbjct: 181 LYLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSS 240

Query: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300
           WLAYVIFEDTQQGK++FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLL
Sbjct: 241 WLAYVIFEDTQQGKIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLL 300

Query: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILL 360
           GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAF LMDTNNVGFINKEQCL LL
Sbjct: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALL 360

Query: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420
           EELNRYR+LPK+SRDDYELIFDELDDSRDFKINMNEF DLCNAIALKFQKEDIPSWFEGY
Sbjct: 361 EELNRYRSLPKVSRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGY 420

Query: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVF 480
           PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNL+AVIIETTLDI NN GQKAWQELEFVF
Sbjct: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVF 480

Query: 481 GWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540
           GWIYVVEMALKIY+FGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR
Sbjct: 481 GWIYVVEMALKIYSFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540

Query: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600
           YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYC LGVQVFGG+V
Sbjct: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCNLGVQVFGGLV 600

Query: 601 NAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVY 660
           NAGN TLSKTDL++DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKELTGSIW+LVY
Sbjct: 601 NAGNATLSKTDLDEDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVY 660

Query: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSR 720
           FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTK+RSR
Sbjct: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSR 720

Query: 721 KVDILLHHML 731
           KVDILLHHML
Sbjct: 721 KVDILLHHML 728

BLAST of CaUC07G127770 vs. ExPASy Swiss-Prot
Match: Q75VR1 (Two pore calcium channel protein 1A OS=Nicotiana tabacum OX=4097 GN=TPC1A PE=2 SV=1)

HSP 1 Score: 1016.5 bits (2627), Expect = 1.6e-295
Identity = 513/730 (70.27%), Postives = 619/730 (84.79%), Query Frame = 0

Query: 1   MEESLLGGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60
           MEE LL   GESS+S  +RR+I  +  RR  DAIA+GSAYQ+AAALVDLAEDGIG+PE+I
Sbjct: 1   MEEYLL--SGESSNSGRTRRRIGSIFDRR--DAIAHGSAYQKAAALVDLAEDGIGLPEEI 60

Query: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120
           L+ ++F+ AA+LYFIF +FD +WSLN+ AL+VLNFFEKPLWCSK+   SCN+R+Y++LG+
Sbjct: 61  LEGASFEKAAELYFIFTRFDFLWSLNYLALVVLNFFEKPLWCSKHLAESCNNRDYYYLGE 120

Query: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYT 180
           LP+LT  ESLI+E VTL++L+IH  FPISYEG  LYW+++LN++KVI + ILVAD +VY 
Sbjct: 121 LPFLTGAESLIFEGVTLLLLIIHILFPISYEGFNLYWRSLLNRVKVILLLILVADIVVYI 180

Query: 181 LYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSS 240
           L+L+   F YLP R+APY+RVVFFI+NIR LR+S  ILAGMLGTYLNV+AL  LFLLFSS
Sbjct: 181 LFLAD--FYYLPFRIAPYLRVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFSS 240

Query: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300
           WLAYV FEDT+QGK  FTS+G TLYQMF+LFTTSNNPDVWIPAYK SRWYCLFFVLYVLL
Sbjct: 241 WLAYVFFEDTRQGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLL 300

Query: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILL 360
           GVYFVTNLILAVVYDSFKS+L KQ+++ DRLR   L KAFSL+D  N G +N++QC +L 
Sbjct: 301 GVYFVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGHLNEKQCTLLF 360

Query: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420
           EELN+YRTLPKIS DD++ IF ELDD+ DFKIN++EF DLC AI L+FQKED    FE  
Sbjct: 361 EELNKYRTLPKISGDDFKSIFSELDDTGDFKINLDEFADLCTAIGLRFQKEDSLPIFEAC 420

Query: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVF 480
           P+ YHS  S+KL+ FVR  TF Y + F+L++NL+AVIIETTLDI+NN GQ  WQ++EF F
Sbjct: 421 PNFYHSPASEKLRGFVRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTF 480

Query: 481 GWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540
           GW+YV+EMALK+Y +GF NYWRDGQN+FDF+VTW+IVIGET TFVAP GLTFLSNGEWIR
Sbjct: 481 GWLYVIEMALKVYTYGFENYWRDGQNRFDFVVTWVIVIGETATFVAPDGLTFLSNGEWIR 540

Query: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600
           YLLIARMLRLIRLLMHV +YRAF+ATF TLIPSL+PYLGTIFC+LC YC+LG+Q+FGGIV
Sbjct: 541 YLLIARMLRLIRLLMHVERYRAFVATFFTLIPSLVPYLGTIFCILCFYCSLGLQIFGGIV 600

Query: 601 NAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVY 660
           N GNP L++TDL  +DYLLFNFNDYPNGMVTLFN+LVMGNWQ WMQSYKELTG+ W+  Y
Sbjct: 601 NTGNPNLAQTDLAGNDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTAWTYAY 660

Query: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSR 720
           F+SFYLI+VL LLNL+VAFVLEAF AE+D+E+S    + D D +++ +RRR VGTK+RS+
Sbjct: 661 FVSFYLISVLWLLNLIVAFVLEAFQAEVDLEASARCVDGD-DKEAKSERRRNVGTKTRSQ 720

Query: 721 KVDILLHHML 731
           +VD LLHHML
Sbjct: 721 RVDFLLHHML 723

BLAST of CaUC07G127770 vs. ExPASy Swiss-Prot
Match: Q75VR0 (Two pore calcium channel protein 1B OS=Nicotiana tabacum OX=4097 GN=TPC1B PE=2 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 1.3e-294
Identity = 512/730 (70.14%), Postives = 618/730 (84.66%), Query Frame = 0

Query: 1   MEESLLGGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60
           MEE LL   GESS+S  +RR+   +  RR  DAIA+GSAYQ+AAALVDLAEDGIG+PE+I
Sbjct: 1   MEEYLL--PGESSNSCRTRRRSGSIFDRR--DAIAHGSAYQKAAALVDLAEDGIGLPEEI 60

Query: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120
           L+ ++F+ AA+LYF+F +FD +WSLN+ AL+VLNFFEKPLWCSK+   SCN+R+Y++LG+
Sbjct: 61  LEGASFEKAAELYFMFTRFDFLWSLNYLALVVLNFFEKPLWCSKHLAESCNNRDYYYLGE 120

Query: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYT 180
           LP+LT  ESLI+E VTL++L+IH  FPISYEG  LYW+++LN+LKVI + ILVAD +VY 
Sbjct: 121 LPFLTGAESLIFEGVTLLLLIIHILFPISYEGFNLYWRSLLNRLKVILLLILVADIVVYI 180

Query: 181 LYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSS 240
           L   P  F YLP R+APY+RVVFFI+NIR LR+S  ILAGMLGTYLNV+AL  LFLLFSS
Sbjct: 181 LL--PADFYYLPFRIAPYLRVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFSS 240

Query: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300
           WLAYV FEDT+QGK  FTS+G TLYQMF+LFTTSNNPDVWIPAYK SRWYCLFFVLYVLL
Sbjct: 241 WLAYVFFEDTRQGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLL 300

Query: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILL 360
           GVYFVTNLILAVVYDSFKS+L KQ+++ DRLR   L KAFSL+D  N G +N++QC +L 
Sbjct: 301 GVYFVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGLLNEKQCTLLF 360

Query: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420
           EELN+YRTLPKIS DD++ IF+ELDD+ DFKIN+ EF DLC+AI L+FQKED    FE  
Sbjct: 361 EELNKYRTLPKISGDDFKSIFNELDDTGDFKINLEEFADLCSAIGLRFQKEDSLPIFEAC 420

Query: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVF 480
           P+ YHS  S+KL+ F+R  TF Y + F+L++NL+AVIIETTLDI+NN GQ  WQ++EF F
Sbjct: 421 PNFYHSPASEKLRGFIRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTF 480

Query: 481 GWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540
           GW+YV+EMALK+Y +GF NYWRDGQN+FDF+VTW+IVIGET TFVAP  LTFLSNGEWIR
Sbjct: 481 GWLYVIEMALKVYTYGFENYWRDGQNRFDFIVTWVIVIGETTTFVAPDDLTFLSNGEWIR 540

Query: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600
           YLLIARMLRLIRLLMHV +YRAF+ATFLTLIPSLMPYLGTIFC+LC YC+LG+Q+FGGIV
Sbjct: 541 YLLIARMLRLIRLLMHVERYRAFVATFLTLIPSLMPYLGTIFCILCFYCSLGLQIFGGIV 600

Query: 601 NAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVY 660
           N GNP L++TDL  +DYLLFNFNDYPNGMVTLFN+LVMGNWQ WMQSYKELTG+ W+  Y
Sbjct: 601 NTGNPNLAQTDLAGNDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTSWTYAY 660

Query: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSR 720
           F+SFYLI+VL LLNL+VAFVLEAF AE+D+E+S    + D D +++++RRR VGTK+RS+
Sbjct: 661 FVSFYLISVLWLLNLIVAFVLEAFQAEMDLEASARCVDGD-DKEAKRERRRNVGTKTRSQ 720

Query: 721 KVDILLHHML 731
           +VD LLHHML
Sbjct: 721 RVDFLLHHML 723

BLAST of CaUC07G127770 vs. ExPASy Swiss-Prot
Match: Q94KI8 (Two pore calcium channel protein 1 OS=Arabidopsis thaliana OX=3702 GN=TPC1 PE=1 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 1.2e-292
Identity = 489/702 (69.66%), Postives = 607/702 (86.47%), Query Frame = 0

Query: 29  RRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILDQSNFQSAAKLYFIFIKFDLIWSLNFF 88
           RRS+AI +G+ +Q+AAALVDLAEDGIG+P +ILDQS+F  +A+ YFIF + DLIWSLN+F
Sbjct: 20  RRSEAITHGTPFQKAAALVDLAEDGIGLPVEILDQSSFGESARYYFIFTRLDLIWSLNYF 79

Query: 89  ALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQLPYLTAVESLIYEAVTLIILMIHTFFPI 148
           AL+ LNFFE+PLWC K    SC DR+Y++LG+LPYLT  ES+IYE +TL IL++HTFFPI
Sbjct: 80  ALLFLNFFEQPLWCEKNPKPSCKDRDYYYLGELPYLTNAESIIYEVITLAILLVHTFFPI 139

Query: 149 SYEGAQLYWKNILNQLKVICITILVADFLVYTLYLSPVAFDYLPVRVAPYIRVVFFIMNI 208
           SYEG++++W + LN +KV C+ IL  D LV  LYLSP+AFD+LP R+APY+RV+ FI++I
Sbjct: 140 SYEGSRIFWTSRLNLVKVACVVILFVDVLVDFLYLSPLAFDFLPFRIAPYVRVIIFILSI 199

Query: 209 RHLRESIIILAGMLGTYLNVMALWLLFLLFSSWLAYVIFEDTQQGKLIFTSFGATLYQMF 268
           R LR+++++L+GMLGTYLN++ALW+LFLLF+SW+A+V+FEDTQQG  +FTS+GATLYQMF
Sbjct: 200 RELRDTLVLLSGMLGTYLNILALWMLFLLFASWIAFVMFEDTQQGLTVFTSYGATLYQMF 259

Query: 269 ILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVYDSFKSQLAKQISEM 328
           ILFTTSNNPDVWIPAYK+SRW  +FFVLYVL+GVYFVTNLILAVVYDSFK QLAKQ+S M
Sbjct: 260 ILFTTSNNPDVWIPAYKSSRWSSVFFVLYVLIGVYFVTNLILAVVYDSFKEQLAKQVSGM 319

Query: 329 DRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKISRDDYELIFDELDDSR 388
           D++++ +L KAF L+D++  G I+K QC+ L E+L  YRTLPKIS++++ LIFDELDD+R
Sbjct: 320 DQMKRRMLEKAFGLIDSDKNGEIDKNQCIKLFEQLTNYRTLPKISKEEFGLIFDELDDTR 379

Query: 389 DFKINMNEFDDLCNAIALKFQKEDIPSWFEGYPSIYHSVFSKKLKAFVRSPTFGYAVSFI 448
           DFKIN +EF DLC AIAL+FQKE++PS FE +P IYHS  S++L+AFVRSP FGYA+SFI
Sbjct: 380 DFKINKDEFADLCQAIALRFQKEEVPSLFEHFPQIYHSALSQQLRAFVRSPNFGYAISFI 439

Query: 449 LILNLIAVIIETTLDIENNVGQKAWQELEFVFGWIYVVEMALKIYAFGFLNYWRDGQNQF 508
           LI+N IAV++ETTLDIE +  QK WQ  EFVFGWIYV+EMALKIY +GF NYWR+G N+F
Sbjct: 440 LIINFIAVVVETTLDIEESSAQKPWQVAEFVFGWIYVLEMALKIYTYGFENYWREGANRF 499

Query: 509 DFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRLLMHVRQYRAFLATFL 568
           DFLVTW+IVIGET TF+ P   TF SNGEWIRYLL+ARMLRLIRLLM+V++YRAF+ATF+
Sbjct: 500 DFLVTWVIVIGETATFITPDENTFFSNGEWIRYLLLARMLRLIRLLMNVQRYRAFIATFI 559

Query: 569 TLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNPTLSKTDLNDDDYLLFNFNDYPNG 628
           TLIPSLMPYLGTIFCVLC+YC++GVQVFGG+VNAGN  L +T+L +DDYLLFNFNDYPNG
Sbjct: 560 TLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKKLFETELAEDDYLLFNFNDYPNG 619

Query: 629 MVTLFNLLVMGNWQDWMQSYKELTGSIWSLVYFISFYLITVLLLLNLVVAFVLEAFFAEL 688
           MVTLFNLLVMGNWQ WM+SYK+LTG+ WS+ YF+SFY+IT+LLLLNLVVAFVLEAFF EL
Sbjct: 620 MVTLFNLLVMGNWQVWMESYKDLTGTWWSITYFVSFYVITILLLLNLVVAFVLEAFFTEL 679

Query: 689 DIESSENGEEQDQDGDSRKDRRRFVGTKSRSRKVDILLHHML 731
           D+E  E  + Q QD   +++RRR  G+KSRS++VD LLHHML
Sbjct: 680 DLEEEE--KCQGQDSQEKRNRRRSAGSKSRSQRVDTLLHHML 719

BLAST of CaUC07G127770 vs. ExPASy Swiss-Prot
Match: Q5QM84 (Two pore calcium channel protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=TPC1 PE=1 SV=2)

HSP 1 Score: 934.9 bits (2415), Expect = 6.0e-271
Identity = 468/724 (64.64%), Postives = 578/724 (79.83%), Query Frame = 0

Query: 7   GGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILDQSNF 66
           GGGG   S           +++RRSDA+AYG+ YQ+AAALVDLAEDG+GIPE +L+ + F
Sbjct: 40  GGGGWRGSR----------QYQRRSDALAYGNRYQKAAALVDLAEDGVGIPEDVLNDTRF 99

Query: 67  QSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQLPYLTA 126
           + A + YF++++ D +WSLN FALI+LNF EKPLWC  Y  H+C+ R+ +FLGQLPYL+ 
Sbjct: 100 ERAMRFYFVYLRLDWLWSLNLFALILLNFLEKPLWCRGYSQHACDQRDLYFLGQLPYLSK 159

Query: 127 VESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYTLYLSPV 186
            ESLIYE +TL+IL++  F+P+SYEG  L+WKN +N+LKV+ + IL  D LV+       
Sbjct: 160 TESLIYEGLTLVILVMDIFYPLSYEGLNLFWKNTINKLKVLLLFILACDILVF------- 219

Query: 187 AFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSSWLAYVI 246
           AF   P RVAPYIRV F IMNIR LR   + L GM+GTYLNV+AL LLFLLF+SWLAYV 
Sbjct: 220 AFSPQPFRVAPYIRVAFLIMNIRELRMCAVTLVGMVGTYLNVLALSLLFLLFASWLAYVT 279

Query: 247 FEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVT 306
           FEDT QGK +F+S+G TLYQMFILFTTSNNPDVW+PAYK+SRW  LFF++YVLLGVYF+T
Sbjct: 280 FEDTPQGKTVFSSYGTTLYQMFILFTTSNNPDVWVPAYKSSRWSSLFFIVYVLLGVYFLT 339

Query: 307 NLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRY 366
           NLILAV+YDSFK QLAKQ+S+ D  RKSIL KAF ++D    G++NKEQCL LL+ELN+Y
Sbjct: 340 NLILAVIYDSFKEQLAKQVSQADCTRKSILEKAFGIIDATGQGYLNKEQCLSLLDELNKY 399

Query: 367 RTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGYPSIYHS 426
           R+LPK SR+D+ELIF ELD S DFK+   EF  LCN IA+KFQKE  PS+ E YPS YHS
Sbjct: 400 RSLPKTSREDFELIFAELDQSGDFKVTSEEFATLCNTIAIKFQKEPPPSYLEKYPSFYHS 459

Query: 427 VFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVFGWIYVV 486
              + LK+FVRSP F Y V F+L++NL+AVIIETTLDIEN+  QK WQE+EFVFGWIYV+
Sbjct: 460 ALCEWLKSFVRSPLFEYIVIFVLLMNLVAVIIETTLDIENSSSQKVWQEVEFVFGWIYVI 519

Query: 487 EMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIAR 546
           EMALKI++ GF  YW +GQN+FDF++TW I IGET+TF  PS L+FLSNGEWIRYLL+ R
Sbjct: 520 EMALKIFSLGFGAYWMEGQNKFDFVLTWTIFIGETLTFAFPSKLSFLSNGEWIRYLLLGR 579

Query: 547 MLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNPT 606
           MLRL R+L+ VR++RAF+ATF TL+ SLMPYLG +FC LC+YC+LG+Q+FGGIV AGNPT
Sbjct: 580 MLRLTRILLQVRRFRAFVATFFTLMSSLMPYLGIVFCTLCIYCSLGLQIFGGIVYAGNPT 639

Query: 607 LSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVYFISFYL 666
           L +TDL  +DYLLFNFNDYP+GMVTLFNLLVMGNWQ WM+SY++LTGS WSL+YF+SFYL
Sbjct: 640 LEETDLFSNDYLLFNFNDYPSGMVTLFNLLVMGNWQAWMESYRQLTGSYWSLIYFVSFYL 699

Query: 667 ITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSRKVDILL 726
           I+VLLLLNL+VAFVLEAFFAE+++E     + QD   + R +RRR V  +++   VDILL
Sbjct: 700 ISVLLLLNLIVAFVLEAFFAEMELEKDGEADIQDPTLEGR-NRRRSVRVRTKGTMVDILL 745

Query: 727 HHML 731
           HHML
Sbjct: 760 HHML 745

BLAST of CaUC07G127770 vs. ExPASy Swiss-Prot
Match: Q6S5H8 (Two pore calcium channel protein 1 OS=Hordeum vulgare OX=4513 GN=TPC1 PE=2 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 2.4e-259
Identity = 450/724 (62.15%), Postives = 567/724 (78.31%), Query Frame = 0

Query: 7   GGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILDQSNF 66
           GGGG+ S            ++RRRSDA+A+G  YQ+AAALVDLAEDG+GIPE +L+ + F
Sbjct: 29  GGGGQGSR-----------KYRRRSDALAHGDRYQKAAALVDLAEDGVGIPEDVLNDTRF 88

Query: 67  QSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQLPYLTA 126
             A   YF++++ D +WSLN FALI+LNF EKPLWC K   H+C+ R+ +FLGQLPY + 
Sbjct: 89  GRAMSFYFVYLRLDWLWSLNIFALILLNFLEKPLWCRKDALHACDQRDMYFLGQLPYFSK 148

Query: 127 VESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYTLYLSPV 186
            ESLIYE +TL+IL++    P+SYEG  ++W++  N+LK++ + IL  D LV+       
Sbjct: 149 TESLIYEGLTLVILVMEILCPLSYEGLNIFWRSTTNKLKILLLFILACDILVF------- 208

Query: 187 AFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSSWLAYVI 246
           AF   P R+APYIRVVF IM IR LR   I LAG++GTYLNV+AL LLFLLF+SWLAYV 
Sbjct: 209 AFSSQPFRLAPYIRVVFLIMTIRELRMCAITLAGLIGTYLNVLALSLLFLLFASWLAYVT 268

Query: 247 FEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVT 306
           FEDT QGK IF+S+G TLYQMF+LFTTSNNPDVW+PAYK SRWY LFF++YVLLGVYF+T
Sbjct: 269 FEDTPQGKTIFSSYGVTLYQMFVLFTTSNNPDVWVPAYKISRWYSLFFIVYVLLGVYFLT 328

Query: 307 NLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRY 366
           NLILAV+YDSFK Q AKQ+ ++D +RK+IL KAF L+DTN  G++++EQC+ LL ELN+Y
Sbjct: 329 NLILAVIYDSFKEQFAKQLVQVDAIRKNILQKAFELIDTNTRGYLDREQCISLLNELNKY 388

Query: 367 RTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGYPSIYHS 426
           R+LPK SR+D+ELIF ELD S DFK+   EF DLCN IA+KFQKE  PS+ E +P  YHS
Sbjct: 389 RSLPKTSREDFELIFAELDRSGDFKVTSEEFADLCNTIAIKFQKEPPPSYLEKFP-FYHS 448

Query: 427 VFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVFGWIYVV 486
               +LK+FVRS TF Y + F+L++NL+AVIIETTLDIEN+  Q+ WQE+EF  GWIYV 
Sbjct: 449 PVCGRLKSFVRSRTFEYIIVFVLLINLVAVIIETTLDIENSSSQETWQEVEFFLGWIYVA 508

Query: 487 EMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIAR 546
           EMALKI++ GF  YW +GQN+FDF++TW I IGET+TF  PS L FLSNGEWIRYLL+ R
Sbjct: 509 EMALKIFSLGFGAYWMEGQNKFDFVLTWTIFIGETLTFAFPSKLPFLSNGEWIRYLLLGR 568

Query: 547 MLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNPT 606
           +LRL R+L+ V+++R F+ATF TL+ SLMPYLG +FC+LC+YC+LG+Q+FGGIV AGNPT
Sbjct: 569 VLRLTRILLQVQRFRVFVATFFTLMSSLMPYLGIVFCILCMYCSLGLQIFGGIVYAGNPT 628

Query: 607 LSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVYFISFYL 666
           L +TDL  +DYLLFNFNDYP+GMVTLFNLLVMGNWQ WM+SY +LTGS WSL+YF+SFYL
Sbjct: 629 LEETDLFSNDYLLFNFNDYPSGMVTLFNLLVMGNWQVWMESYWQLTGSSWSLIYFVSFYL 688

Query: 667 ITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSRKVDILL 726
           I++LLLLNL+VAFVLEAFFAE+++E  E  + Q       K +RR +  +S+   VDILL
Sbjct: 689 ISILLLLNLIVAFVLEAFFAEMELEKGEEVDIQSPTSGGIK-KRRSMRVRSKGTMVDILL 732

Query: 727 HHML 731
           HHML
Sbjct: 749 HHML 732

BLAST of CaUC07G127770 vs. ExPASy TrEMBL
Match: A0A1S3C1V7 (two pore calcium channel protein 1B isoform X1 OS=Cucumis melo OX=3656 GN=LOC103495512 PE=3 SV=1)

HSP 1 Score: 1369.0 bits (3542), Expect = 0.0e+00
Identity = 702/733 (95.77%), Postives = 716/733 (97.68%), Query Frame = 0

Query: 1   MEESLL---GGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIP 60
           MEESLL   GGGGESS SS+SRRQ PHVRFRRRSDAIAYGSAYQRAAALVDLAEDG+GIP
Sbjct: 1   MEESLLGGGGGGGESSGSSTSRRQTPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGVGIP 60

Query: 61  EKILDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFF 120
           EKILDQSNFQSAAKLYFIFIKFD IWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFF
Sbjct: 61  EKILDQSNFQSAAKLYFIFIKFDWIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFF 120

Query: 121 LGQLPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFL 180
           LGQLPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYW N+LNQLKVICI ILVADFL
Sbjct: 121 LGQLPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWTNLLNQLKVICIIILVADFL 180

Query: 181 VYTLYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLL 240
           VYTLYLSPVAFDYLP R APYIRV+FFIMNIRHLRESIIILAGMLGTYLNV+ALW LFLL
Sbjct: 181 VYTLYLSPVAFDYLPFRAAPYIRVIFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLL 240

Query: 241 FSSWLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLY 300
           FSSWLAYVIFEDTQQGKLIFTSFG TLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLY
Sbjct: 241 FSSWLAYVIFEDTQQGKLIFTSFGTTLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLY 300

Query: 301 VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCL 360
           VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCL
Sbjct: 301 VLLGVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCL 360

Query: 361 ILLEELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWF 420
           +LLEELNRYRTLPKISRDDYELIFDELDDSRDFKIN+NEFDDLCNAIALKFQKEDIPSWF
Sbjct: 361 MLLEELNRYRTLPKISRDDYELIFDELDDSRDFKININEFDDLCNAIALKFQKEDIPSWF 420

Query: 421 EGYPSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELE 480
           EGYPSIYHS FSKKLKAFVRSP FGYAVSFILILNL+AVIIETTLDIENNVGQKAWQELE
Sbjct: 421 EGYPSIYHSSFSKKLKAFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELE 480

Query: 481 FVFGWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGE 540
           FVFGWIYVVEMALKIYA+GF+NYWRDGQNQFDFL+TWIIVIGETITFVAPSGLTFLSNGE
Sbjct: 481 FVFGWIYVVEMALKIYAYGFMNYWRDGQNQFDFLITWIIVIGETITFVAPSGLTFLSNGE 540

Query: 541 WIRYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG 600
           WIRYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG
Sbjct: 541 WIRYLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFG 600

Query: 601 GIVNAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS 660
           GIVNAGNPTL KTDL+DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS
Sbjct: 601 GIVNAGNPTLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS 660

Query: 661 LVYFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKS 720
           LVYFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQD DSRKDR RFVGTK+
Sbjct: 661 LVYFISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDSDSRKDRPRFVGTKT 720

Query: 721 RSRKVDILLHHML 731
           RSRKVDILLHHML
Sbjct: 721 RSRKVDILLHHML 733

BLAST of CaUC07G127770 vs. ExPASy TrEMBL
Match: A0A0A0K5Q7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G048010 PE=3 SV=1)

HSP 1 Score: 1363.2 bits (3527), Expect = 0.0e+00
Identity = 699/730 (95.75%), Postives = 716/730 (98.08%), Query Frame = 0

Query: 1   MEESLLGGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60
           MEESLLGGGGESS SS+SRR  PHVRFRRRSDAIAYGSAYQRAAALVDLAEDG+GIPEKI
Sbjct: 1   MEESLLGGGGESSGSSTSRR--PHVRFRRRSDAIAYGSAYQRAAALVDLAEDGVGIPEKI 60

Query: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120
           LDQSNFQSAAKLYFIFIKFD IWSLNFFALIVLNFFEKPLWC KYDTHSCNDREYFFLGQ
Sbjct: 61  LDQSNFQSAAKLYFIFIKFDWIWSLNFFALIVLNFFEKPLWCFKYDTHSCNDREYFFLGQ 120

Query: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYT 180
           LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYW N+LNQLKVICITILVADFLVYT
Sbjct: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWTNLLNQLKVICITILVADFLVYT 180

Query: 181 LYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSS 240
           LYLSPVAFDYLP R APYIRVVFFIMNIRHLRESIIILAGMLGTYLNV+ALW LFLLFSS
Sbjct: 181 LYLSPVAFDYLPFRAAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSS 240

Query: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300
           WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLL
Sbjct: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLL 300

Query: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILL 360
           GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVG+INKEQCL+LL
Sbjct: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGYINKEQCLMLL 360

Query: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420
           EELNRYR+LPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY
Sbjct: 361 EELNRYRSLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420

Query: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVF 480
           PS+YHS  SKKLKAFVRSP FGYAVSFIL+LNL+AVIIETTLDIENNVGQKAWQELEFVF
Sbjct: 421 PSVYHSGSSKKLKAFVRSPKFGYAVSFILVLNLVAVIIETTLDIENNVGQKAWQELEFVF 480

Query: 481 GWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540
           GWIYVVEMALKIYA+GF+NYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR
Sbjct: 481 GWIYVVEMALKIYAYGFMNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540

Query: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600
           YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV
Sbjct: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600

Query: 601 NAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVY 660
           NAGNPTLSKT+L+DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSY+ELTGSIWSLVY
Sbjct: 601 NAGNPTLSKTELDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYRELTGSIWSLVY 660

Query: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSR 720
           FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQD DSRKDR RFVGTK+RSR
Sbjct: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDKDSRKDRPRFVGTKTRSR 720

Query: 721 KVDILLHHML 731
           KVDILLHHML
Sbjct: 721 KVDILLHHML 728

BLAST of CaUC07G127770 vs. ExPASy TrEMBL
Match: A0A6J1ERC4 (two pore calcium channel protein 1A-like OS=Cucurbita moschata OX=3662 GN=LOC111437105 PE=3 SV=1)

HSP 1 Score: 1348.2 bits (3488), Expect = 0.0e+00
Identity = 692/730 (94.79%), Postives = 716/730 (98.08%), Query Frame = 0

Query: 1   MEESLLGGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60
           M+ESLL  G ESSSSSSSRRQIPHV+F+RRSDAIAYGSAYQRAAALVDLAEDG+GIPEKI
Sbjct: 1   MDESLL--GVESSSSSSSRRQIPHVKFKRRSDAIAYGSAYQRAAALVDLAEDGVGIPEKI 60

Query: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120
           LDQSNFQSAAKLYFIFIKFDLIWS+NFFAL+VLNFFEKPLWCSKYDTHSCNDREYFFLGQ
Sbjct: 61  LDQSNFQSAAKLYFIFIKFDLIWSVNFFALVVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120

Query: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYT 180
           LPYLTAVESLIYEAVTLIILMIHTFFPIS+EGAQLYWKN LNQLKVIC+ ILVADFL+YT
Sbjct: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISFEGAQLYWKNPLNQLKVICLIILVADFLIYT 180

Query: 181 LYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSS 240
           LYLSPVAFDYLP RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNV+ALW LFLLFSS
Sbjct: 181 LYLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSS 240

Query: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300
           WLAYVIFEDTQQGKL+FTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLL
Sbjct: 241 WLAYVIFEDTQQGKLLFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLL 300

Query: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILL 360
           GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDT+NVGFINK+QCLILL
Sbjct: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTDNVGFINKQQCLILL 360

Query: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420
           EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEF DLCNAIALKFQKEDIPS FE Y
Sbjct: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSVFEAY 420

Query: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVF 480
           PSIY+SV SKKLKAFVRSP+FGYA+SFILILNL+AVIIETTLDIENNVGQKAWQELEFVF
Sbjct: 421 PSIYNSVSSKKLKAFVRSPSFGYAISFILILNLVAVIIETTLDIENNVGQKAWQELEFVF 480

Query: 481 GWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540
           GWIYVVEMALKIYAFGF+NYWRDGQNQFDFLVTWIIVIGET+TFVAPSGLTFLSNGEWIR
Sbjct: 481 GWIYVVEMALKIYAFGFMNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIR 540

Query: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600
           YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+V
Sbjct: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLV 600

Query: 601 NAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVY 660
           NAGNP LSKTDL+DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS VY
Sbjct: 601 NAGNPALSKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSHVY 660

Query: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSR 720
           FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQ+QD DSRKDRRRFVGTK+RSR
Sbjct: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQEQDADSRKDRRRFVGTKTRSR 720

Query: 721 KVDILLHHML 731
           KVDILLHHML
Sbjct: 721 KVDILLHHML 728

BLAST of CaUC07G127770 vs. ExPASy TrEMBL
Match: A0A6J1HWG3 (two pore calcium channel protein 1A-like OS=Cucurbita maxima OX=3661 GN=LOC111468128 PE=3 SV=1)

HSP 1 Score: 1346.3 bits (3483), Expect = 0.0e+00
Identity = 689/730 (94.38%), Postives = 711/730 (97.40%), Query Frame = 0

Query: 1   MEESLLGGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60
           MEESLL  GGE SSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI
Sbjct: 1   MEESLL--GGEGSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60

Query: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120
           LDQSNFQSAA+LYFIFI+FD +WSLNFFALI+LNFFE+PLWCSKY+THSCNDREYFFLGQ
Sbjct: 61  LDQSNFQSAARLYFIFIRFDFLWSLNFFALIILNFFEEPLWCSKYETHSCNDREYFFLGQ 120

Query: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYT 180
           LPYLTAVESLIYEA TLIIL IHTFFPISYEGAQLYWKN LNQLKV+C  ILVADF++YT
Sbjct: 121 LPYLTAVESLIYEATTLIILTIHTFFPISYEGAQLYWKNPLNQLKVVCTIILVADFVIYT 180

Query: 181 LYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSS 240
           LYLSPVAFDYLP RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNV+AL LLFLLFSS
Sbjct: 181 LYLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALGLLFLLFSS 240

Query: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300
           WLAYVIFEDTQQGK++FTSFGATLYQMFILFTTSN+PDVWIPAYKASRWYCLFFVLYVLL
Sbjct: 241 WLAYVIFEDTQQGKIVFTSFGATLYQMFILFTTSNSPDVWIPAYKASRWYCLFFVLYVLL 300

Query: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILL 360
           GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAF LMDTNNVGFINKEQCL LL
Sbjct: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFGLMDTNNVGFINKEQCLALL 360

Query: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420
           EELNRYR+LPK+SRDDYELIFDELDDSRDFKINMNEF DLCNAIALKFQKEDIPSWFEGY
Sbjct: 361 EELNRYRSLPKVSRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSWFEGY 420

Query: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVF 480
           PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNL+AVIIETTLDI NN GQKAWQELEFVF
Sbjct: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLVAVIIETTLDIANNAGQKAWQELEFVF 480

Query: 481 GWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540
           GWIYVVEMALKIY+FGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR
Sbjct: 481 GWIYVVEMALKIYSFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540

Query: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600
           YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYC LGVQVFGG+V
Sbjct: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCNLGVQVFGGLV 600

Query: 601 NAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVY 660
           NAGN TLSKTDL++DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKELTGSIW+LVY
Sbjct: 601 NAGNATLSKTDLDEDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGSIWTLVY 660

Query: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSR 720
           FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTK+RSR
Sbjct: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKTRSR 720

Query: 721 KVDILLHHML 731
           KVDILLHHML
Sbjct: 721 KVDILLHHML 728

BLAST of CaUC07G127770 vs. ExPASy TrEMBL
Match: A0A6J1JDS0 (two pore calcium channel protein 1A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485952 PE=3 SV=1)

HSP 1 Score: 1344.3 bits (3478), Expect = 0.0e+00
Identity = 691/730 (94.66%), Postives = 713/730 (97.67%), Query Frame = 0

Query: 1   MEESLLGGGGESSSSSSSRRQIPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGIGIPEKI 60
           M+ESLL  G ES SSSSSRRQIPHV+F+RRSDAIAYGSAYQRAAALVDLAEDG+GIPEKI
Sbjct: 1   MDESLL--GVESGSSSSSRRQIPHVKFKRRSDAIAYGSAYQRAAALVDLAEDGVGIPEKI 60

Query: 61  LDQSNFQSAAKLYFIFIKFDLIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120
           LDQSNFQSAAKLYFIFIKFDLIWS+NFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ
Sbjct: 61  LDQSNFQSAAKLYFIFIKFDLIWSVNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQ 120

Query: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISYEGAQLYWKNILNQLKVICITILVADFLVYT 180
           LPYLTAVESLIYEAVTLIILMIHTFFPIS+EGAQLYWKN LNQLKVIC+ ILVADFL+YT
Sbjct: 121 LPYLTAVESLIYEAVTLIILMIHTFFPISFEGAQLYWKNPLNQLKVICLIILVADFLIYT 180

Query: 181 LYLSPVAFDYLPVRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVMALWLLFLLFSS 240
           LYLSPVAFDYLP RVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNV+ALW LFLLFSS
Sbjct: 181 LYLSPVAFDYLPFRVAPYIRVVFFIMNIRHLRESIIILAGMLGTYLNVLALWFLFLLFSS 240

Query: 241 WLAYVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPDVWIPAYKASRWYCLFFVLYVLL 300
           WLAYVIFEDT+QGKL+FTSFGATLYQMFILFTTSNNP+VWIPAYKASRWYCLFFVLYVLL
Sbjct: 241 WLAYVIFEDTEQGKLLFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLL 300

Query: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLILL 360
           GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDT+NVGFINK+QCLILL
Sbjct: 301 GVYFVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTDNVGFINKQQCLILL 360

Query: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGY 420
           EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEF DLCNAIALKFQKEDIPS FE Y
Sbjct: 361 EELNRYRTLPKISRDDYELIFDELDDSRDFKINMNEFADLCNAIALKFQKEDIPSVFEAY 420

Query: 421 PSIYHSVFSKKLKAFVRSPTFGYAVSFILILNLIAVIIETTLDIENNVGQKAWQELEFVF 480
           P IY SV SKKLKAFVRSP+FGYA+SFILILNL+AVIIETTLDIENNVGQKAWQELEFVF
Sbjct: 421 PLIYDSVSSKKLKAFVRSPSFGYAISFILILNLVAVIIETTLDIENNVGQKAWQELEFVF 480

Query: 481 GWIYVVEMALKIYAFGFLNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIR 540
           GWIYVVEMALKIYAFGF+NYWRDGQNQFDFLVTWIIVIGET+TFVAPSGLTFLSNGEWIR
Sbjct: 481 GWIYVVEMALKIYAFGFMNYWRDGQNQFDFLVTWIIVIGETMTFVAPSGLTFLSNGEWIR 540

Query: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIV 600
           YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGG+V
Sbjct: 541 YLLIARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGLV 600

Query: 601 NAGNPTLSKTDLNDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVY 660
           NAGNP LSKTDL+DDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWS VY
Sbjct: 601 NAGNPALSKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSHVY 660

Query: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDGDSRKDRRRFVGTKSRSR 720
           FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQD DSRKDRRRFVGTK+RSR
Sbjct: 661 FISFYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDADSRKDRRRFVGTKTRSR 720

Query: 721 KVDILLHHML 731
           KVDILLHHML
Sbjct: 721 KVDILLHHML 728

BLAST of CaUC07G127770 vs. TAIR 10
Match: AT4G03560.1 (two-pore channel 1 )

HSP 1 Score: 1006.9 bits (2602), Expect = 8.8e-294
Identity = 489/702 (69.66%), Postives = 607/702 (86.47%), Query Frame = 0

Query: 29  RRSDAIAYGSAYQRAAALVDLAEDGIGIPEKILDQSNFQSAAKLYFIFIKFDLIWSLNFF 88
           RRS+AI +G+ +Q+AAALVDLAEDGIG+P +ILDQS+F  +A+ YFIF + DLIWSLN+F
Sbjct: 20  RRSEAITHGTPFQKAAALVDLAEDGIGLPVEILDQSSFGESARYYFIFTRLDLIWSLNYF 79

Query: 89  ALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQLPYLTAVESLIYEAVTLIILMIHTFFPI 148
           AL+ LNFFE+PLWC K    SC DR+Y++LG+LPYLT  ES+IYE +TL IL++HTFFPI
Sbjct: 80  ALLFLNFFEQPLWCEKNPKPSCKDRDYYYLGELPYLTNAESIIYEVITLAILLVHTFFPI 139

Query: 149 SYEGAQLYWKNILNQLKVICITILVADFLVYTLYLSPVAFDYLPVRVAPYIRVVFFIMNI 208
           SYEG++++W + LN +KV C+ IL  D LV  LYLSP+AFD+LP R+APY+RV+ FI++I
Sbjct: 140 SYEGSRIFWTSRLNLVKVACVVILFVDVLVDFLYLSPLAFDFLPFRIAPYVRVIIFILSI 199

Query: 209 RHLRESIIILAGMLGTYLNVMALWLLFLLFSSWLAYVIFEDTQQGKLIFTSFGATLYQMF 268
           R LR+++++L+GMLGTYLN++ALW+LFLLF+SW+A+V+FEDTQQG  +FTS+GATLYQMF
Sbjct: 200 RELRDTLVLLSGMLGTYLNILALWMLFLLFASWIAFVMFEDTQQGLTVFTSYGATLYQMF 259

Query: 269 ILFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVYFVTNLILAVVYDSFKSQLAKQISEM 328
           ILFTTSNNPDVWIPAYK+SRW  +FFVLYVL+GVYFVTNLILAVVYDSFK QLAKQ+S M
Sbjct: 260 ILFTTSNNPDVWIPAYKSSRWSSVFFVLYVLIGVYFVTNLILAVVYDSFKEQLAKQVSGM 319

Query: 329 DRLRKSILAKAFSLMDTNNVGFINKEQCLILLEELNRYRTLPKISRDDYELIFDELDDSR 388
           D++++ +L KAF L+D++  G I+K QC+ L E+L  YRTLPKIS++++ LIFDELDD+R
Sbjct: 320 DQMKRRMLEKAFGLIDSDKNGEIDKNQCIKLFEQLTNYRTLPKISKEEFGLIFDELDDTR 379

Query: 389 DFKINMNEFDDLCNAIALKFQKEDIPSWFEGYPSIYHSVFSKKLKAFVRSPTFGYAVSFI 448
           DFKIN +EF DLC AIAL+FQKE++PS FE +P IYHS  S++L+AFVRSP FGYA+SFI
Sbjct: 380 DFKINKDEFADLCQAIALRFQKEEVPSLFEHFPQIYHSALSQQLRAFVRSPNFGYAISFI 439

Query: 449 LILNLIAVIIETTLDIENNVGQKAWQELEFVFGWIYVVEMALKIYAFGFLNYWRDGQNQF 508
           LI+N IAV++ETTLDIE +  QK WQ  EFVFGWIYV+EMALKIY +GF NYWR+G N+F
Sbjct: 440 LIINFIAVVVETTLDIEESSAQKPWQVAEFVFGWIYVLEMALKIYTYGFENYWREGANRF 499

Query: 509 DFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYLLIARMLRLIRLLMHVRQYRAFLATFL 568
           DFLVTW+IVIGET TF+ P   TF SNGEWIRYLL+ARMLRLIRLLM+V++YRAF+ATF+
Sbjct: 500 DFLVTWVIVIGETATFITPDENTFFSNGEWIRYLLLARMLRLIRLLMNVQRYRAFIATFI 559

Query: 569 TLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAGNPTLSKTDLNDDDYLLFNFNDYPNG 628
           TLIPSLMPYLGTIFCVLC+YC++GVQVFGG+VNAGN  L +T+L +DDYLLFNFNDYPNG
Sbjct: 560 TLIPSLMPYLGTIFCVLCIYCSIGVQVFGGLVNAGNKKLFETELAEDDYLLFNFNDYPNG 619

Query: 629 MVTLFNLLVMGNWQDWMQSYKELTGSIWSLVYFISFYLITVLLLLNLVVAFVLEAFFAEL 688
           MVTLFNLLVMGNWQ WM+SYK+LTG+ WS+ YF+SFY+IT+LLLLNLVVAFVLEAFF EL
Sbjct: 620 MVTLFNLLVMGNWQVWMESYKDLTGTWWSITYFVSFYVITILLLLNLVVAFVLEAFFTEL 679

Query: 689 DIESSENGEEQDQDGDSRKDRRRFVGTKSRSRKVDILLHHML 731
           D+E  E  + Q QD   +++RRR  G+KSRS++VD LLHHML
Sbjct: 680 DLEEEE--KCQGQDSQEKRNRRRSAGSKSRSQRVDTLLHHML 719