CaUC02G042900 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC02G042900
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionO-methyltransferase, putative
LocationCiama_Chr02: 30425452 .. 30426804 (-)
RNA-Seq ExpressionCaUC02G042900
SyntenyCaUC02G042900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCTAAAATGCGCCGTCGAACTTCAGTTACCCGATATCATCAACTCCCATGGCTCTCCCATCTCTCTCTCTCAGATTGCTTCCTCCATTGCCGCCTCCAATCCCTCTTCCTCCCCACAAATCTCCTACCTCTCTCGCATCATGAGGTTGTTAGTCCGTCGTAACATCTTCGCCGCTCATCACCCATCCGACGGCGGAGACACCCTCTACGGTCTAACCCACTCCTCCAAATGGCTCCTTCGAGACTCCCCTCTCACCCTCGCTCCCATGGTCTTCTCGGAGCTTCACCAATGGATGGTCAACCCATGGCTCTGTTTCACCCAGGCCGTTAAGGAAGGCGGAAGCCCTTTCCAAATAGCCAACGGCCTTGACATTTGGGATTTCGCTTCCCGAAACCCCCAATTCAACCATTTTTTCAACGACGCCATGGCTTCCACTTCCAAAGTCGTCATGAAGGCCATCCTCTCTGTTTACCAAGATGGGTTCAGCTCTGTTCACTCACTCGCCGACGTGGGCGGCGGAATCGGGGGCTCTGTCTCCGAGATCGTGAAAGCCTTCCCCCACATCAAAGGCATCAATTACGATCTCCCACACGTGGTCAGCACCGCGCCGGTCTATCAAGGAGTGACCCACATCGGAGGAGACATGTTCGAGGACATTCCCAAAGCGGACGCCATTTTTATGAAGGTAAGCGATTGATTGATTGATTGATTCTTTCATTAAAGGGTTTGAAAATGGTGGTCTAAATTTGGAATCGGTGAATGGCAGTGGATATTGCACGATTGGAACGAGGAAGAGTGCGTGAGAATTCTGGTGAATTGCAAGAAAGCGATTCCGGAGAAAACGGGGAAAGTGATTATCGTGGAAGTGGTGTTGAATGAAGATGGGGAAGGGGCATTTGATGACACAAGATTCTACTTCGATCTGCTAATGTTGGCGCATACAAATGGAAAAGAAAGAAGAGAGAAGGAGTGGAAGACAATACTTGAAGAAGCTGGTTTCCCTCGCTACAGACTCATTCCCCTTCCTGCTTTAACTTCCATTATTGAAGCTTATCCATTTTGATTTGTGAATCTGAGTTTCTCCAACTTATTCCACCAATAACTTCCCCCTTACAAACTTTCCACCTTCATTCTTCGTCTTTTACAATTTGGGTCCTCTTTTAAATATTTATATGATTTGATCTTTGTATGTCCCATTTATCCCATCAATAAGTTCCCCTCATAAACTTTGGAGTTGTAAATGGATGATAATGAAATGTTTATAAAGGAACATTATAATATGAGAAAAGAATTCGTTGTCATATCTGTAAATTTGTGACATAGAAATATAGTTTGCAAAGATTTTCAC

mRNA sequence

ATGGCCCTAAAATGCGCCGTCGAACTTCAGTTACCCGATATCATCAACTCCCATGGCTCTCCCATCTCTCTCTCTCAGATTGCTTCCTCCATTGCCGCCTCCAATCCCTCTTCCTCCCCACAAATCTCCTACCTCTCTCGCATCATGAGGTTGTTAGTCCGTCGTAACATCTTCGCCGCTCATCACCCATCCGACGGCGGAGACACCCTCTACGGTCTAACCCACTCCTCCAAATGGCTCCTTCGAGACTCCCCTCTCACCCTCGCTCCCATGGTCTTCTCGGAGCTTCACCAATGGATGGTCAACCCATGGCTCTGTTTCACCCAGGCCGTTAAGGAAGGCGGAAGCCCTTTCCAAATAGCCAACGGCCTTGACATTTGGGATTTCGCTTCCCGAAACCCCCAATTCAACCATTTTTTCAACGACGCCATGGCTTCCACTTCCAAAGTCGTCATGAAGGCCATCCTCTCTGTTTACCAAGATGGGTTCAGCTCTGTTCACTCACTCGCCGACGTGGGCGGCGGAATCGGGGGCTCTGTCTCCGAGATCGTGAAAGCCTTCCCCCACATCAAAGGCATCAATTACGATCTCCCACACGTGGTCAGCACCGCGCCGGTCTATCAAGGAGTGACCCACATCGGAGGAGACATGTTCGAGGACATTCCCAAAGCGGACGCCATTTTTATGAAGTGGATATTGCACGATTGGAACGAGGAAGAGTGCGTGAGAATTCTGGTGAATTGCAAGAAAGCGATTCCGGAGAAAACGGGGAAAGTGATTATCGTGGAAGTGGTGTTGAATGAAGATGGGGAAGGGGCATTTGATGACACAAGATTCTACTTCGATCTGCTAATGTTGGCGCATACAAATGGAAAAGAAAGAAGAGAGAAGGAGTGGAAGACAATACTTGAAGAAGCTGGTTTCCCTCGCTACAGACTCATTCCCCTTCCTGCTTTAACTTCCATTATTGAAGCTTATCCATTTTGATTTGTGAATCTGAGTTTCTCCAACTTATTCCACCAATAACTTCCCCCTTACAAACTTTCCACCTTCATTCTTCGTCTTTTACAATTTGGGTCCTCTTTTAAATATTTATATGATTTGATCTTTGTATGTCCCATTTATCCCATCAATAAGTTCCCCTCATAAACTTTGGAGTTGTAAATGGATGATAATGAAATGTTTATAAAGGAACATTATAATATGAGAAAAGAATTCGTTGTCATATCTGTAAATTTGTGACATAGAAATATAGTTTGCAAAGATTTTCAC

Coding sequence (CDS)

ATGGCCCTAAAATGCGCCGTCGAACTTCAGTTACCCGATATCATCAACTCCCATGGCTCTCCCATCTCTCTCTCTCAGATTGCTTCCTCCATTGCCGCCTCCAATCCCTCTTCCTCCCCACAAATCTCCTACCTCTCTCGCATCATGAGGTTGTTAGTCCGTCGTAACATCTTCGCCGCTCATCACCCATCCGACGGCGGAGACACCCTCTACGGTCTAACCCACTCCTCCAAATGGCTCCTTCGAGACTCCCCTCTCACCCTCGCTCCCATGGTCTTCTCGGAGCTTCACCAATGGATGGTCAACCCATGGCTCTGTTTCACCCAGGCCGTTAAGGAAGGCGGAAGCCCTTTCCAAATAGCCAACGGCCTTGACATTTGGGATTTCGCTTCCCGAAACCCCCAATTCAACCATTTTTTCAACGACGCCATGGCTTCCACTTCCAAAGTCGTCATGAAGGCCATCCTCTCTGTTTACCAAGATGGGTTCAGCTCTGTTCACTCACTCGCCGACGTGGGCGGCGGAATCGGGGGCTCTGTCTCCGAGATCGTGAAAGCCTTCCCCCACATCAAAGGCATCAATTACGATCTCCCACACGTGGTCAGCACCGCGCCGGTCTATCAAGGAGTGACCCACATCGGAGGAGACATGTTCGAGGACATTCCCAAAGCGGACGCCATTTTTATGAAGTGGATATTGCACGATTGGAACGAGGAAGAGTGCGTGAGAATTCTGGTGAATTGCAAGAAAGCGATTCCGGAGAAAACGGGGAAAGTGATTATCGTGGAAGTGGTGTTGAATGAAGATGGGGAAGGGGCATTTGATGACACAAGATTCTACTTCGATCTGCTAATGTTGGCGCATACAAATGGAAAAGAAAGAAGAGAGAAGGAGTGGAAGACAATACTTGAAGAAGCTGGTTTCCCTCGCTACAGACTCATTCCCCTTCCTGCTTTAACTTCCATTATTGAAGCTTATCCATTTTGA

Protein sequence

MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAAHHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQIANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSVSEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEECVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWKTILEEAGFPRYRLIPLPALTSIIEAYPF
Homology
BLAST of CaUC02G042900 vs. NCBI nr
Match: XP_038902222.1 (xanthohumol 4-O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 630.9 bits (1626), Expect = 6.0e-177
Identity = 303/327 (92.66%), Postives = 318/327 (97.25%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL LPDIINSHGSPISLSQIASSIAASNPSSSPQISYL+RIMRLLVRRNIF A
Sbjct: 30  MALKCAVELHLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLTRIMRLLVRRNIFTA 89

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
           HHPS+GG+TLYGLTHSSKWLLRDSPL+LAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI
Sbjct: 90  HHPSNGGETLYGLTHSSKWLLRDSPLSLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 149

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSV 180
           ANGLDIWDFAS+NPQFNHFFNDAMASTSKVVM AILSVY+DGF+SV SLADVGGGIGGSV
Sbjct: 150 ANGLDIWDFASKNPQFNHFFNDAMASTSKVVMNAILSVYKDGFNSVQSLADVGGGIGGSV 209

Query: 181 SEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEE 240
           SEIVKAFPH+KGINYDLPHV+STAP+Y GVTHIGGDMF+ IPKADAIFMKWILHDWN++E
Sbjct: 210 SEIVKAFPHVKGINYDLPHVISTAPLYHGVTHIGGDMFDYIPKADAIFMKWILHDWNDKE 269

Query: 241 CVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWK 300
           CV+IL NCKKAIPEKTGKVIIVEVVLNEDGEG FDDTRFYFDLLMLAHTNGKER EKEWK
Sbjct: 270 CVKILENCKKAIPEKTGKVIIVEVVLNEDGEGTFDDTRFYFDLLMLAHTNGKERTEKEWK 329

Query: 301 TILEEAGFPRYRLIPLPALTSIIEAYP 328
           TILEEAGFPRYR+IPLPALTSIIEAYP
Sbjct: 330 TILEEAGFPRYRVIPLPALTSIIEAYP 356

BLAST of CaUC02G042900 vs. NCBI nr
Match: XP_008448276.2 (PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo] >KAA0062101.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK04926.1 (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 619.4 bits (1596), Expect = 1.8e-173
Identity = 297/327 (90.83%), Postives = 315/327 (96.33%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL L DIINSHGSPISLSQI+SSIAASNPSSSPQISYL+RIMRLLVRRNIFAA
Sbjct: 30  MALKCAVELHLADIINSHGSPISLSQISSSIAASNPSSSPQISYLNRIMRLLVRRNIFAA 89

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
           HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM FSELHQWMV+PWLCFT+AVKEGGSPF+I
Sbjct: 90  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMAFSELHQWMVSPWLCFTEAVKEGGSPFKI 149

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSV 180
           A+GLDIWDFAS+NPQFNHFFNDAMASTSKVVM AILSVYQDGF+S+ SLADVGGGIGGS+
Sbjct: 150 AHGLDIWDFASKNPQFNHFFNDAMASTSKVVMNAILSVYQDGFNSLDSLADVGGGIGGSI 209

Query: 181 SEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEE 240
           SEIVKAFPHIKGINYDLPHV+STAPVY+GVTHIGGDMFEDIPK DAIFMKWILHDWN++E
Sbjct: 210 SEIVKAFPHIKGINYDLPHVISTAPVYEGVTHIGGDMFEDIPKVDAIFMKWILHDWNDKE 269

Query: 241 CVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWK 300
           CV+IL NCKKAIPEK GKVIIVEVVLNE+G+GAFDDT+FYFDLLMLAHTNGKER EKEWK
Sbjct: 270 CVKILENCKKAIPEKRGKVIIVEVVLNEEGKGAFDDTKFYFDLLMLAHTNGKERTEKEWK 329

Query: 301 TILEEAGFPRYRLIPLPALTSIIEAYP 328
           TILEEAGF RY LIPLPAL SIIEAYP
Sbjct: 330 TILEEAGFSRYNLIPLPALVSIIEAYP 356

BLAST of CaUC02G042900 vs. NCBI nr
Match: XP_004139935.1 (xanthohumol 4-O-methyltransferase [Cucumis sativus] >KGN46755.1 hypothetical protein Csa_020899 [Cucumis sativus])

HSP 1 Score: 615.1 bits (1585), Expect = 3.4e-172
Identity = 296/327 (90.52%), Postives = 312/327 (95.41%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL L DIINSHGSPISLSQIASSIAASNPSS+PQISYL+RIMRLLVRRNIFAA
Sbjct: 30  MALKCAVELHLADIINSHGSPISLSQIASSIAASNPSSAPQISYLNRIMRLLVRRNIFAA 89

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
           HHPSDGGD LYGLTHSSKWLLRDSPLTLAPM FSELHQWMVNPWLCFT AVKEGGSPF+I
Sbjct: 90  HHPSDGGDALYGLTHSSKWLLRDSPLTLAPMAFSELHQWMVNPWLCFTGAVKEGGSPFKI 149

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSV 180
           ANGLDIWDFA +NPQFNHFFN+AMASTSKVVM A+LSVY DGF+SVHSLADVGGGIGGS+
Sbjct: 150 ANGLDIWDFAFKNPQFNHFFNNAMASTSKVVMNAVLSVYLDGFNSVHSLADVGGGIGGSI 209

Query: 181 SEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEE 240
           SEIVKA PHIKGINYDLPHVVSTAPVY+GV HIGGDMFEDIPKADAIFMKWILHDWN++E
Sbjct: 210 SEIVKALPHIKGINYDLPHVVSTAPVYEGVIHIGGDMFEDIPKADAIFMKWILHDWNDKE 269

Query: 241 CVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWK 300
           CV+IL NCKKAIPEK GKVIIV+VVLNE+G+GAFDDTRFYFDLLMLAHTNGKER EKEWK
Sbjct: 270 CVKILENCKKAIPEKRGKVIIVDVVLNEEGKGAFDDTRFYFDLLMLAHTNGKERTEKEWK 329

Query: 301 TILEEAGFPRYRLIPLPALTSIIEAYP 328
           TILEEAGF RY+LIPLPAL SIIEAYP
Sbjct: 330 TILEEAGFSRYKLIPLPALVSIIEAYP 356

BLAST of CaUC02G042900 vs. NCBI nr
Match: XP_022946951.1 (xanthohumol 4'-O-methyltransferase-like [Cucurbita moschata])

HSP 1 Score: 572.8 bits (1475), Expect = 1.9e-159
Identity = 273/327 (83.49%), Postives = 299/327 (91.44%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL L DIINSHGSPISLSQIASSI AS+P ++P+ISYLSRIMRLLVRRNIFA 
Sbjct: 33  MALKCAVELHLADIINSHGSPISLSQIASSIVASDPLATPEISYLSRIMRLLVRRNIFAV 92

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
           HH SDGG+ LYGLTHSSKWLLRD+ LTLAPMV +ELH+WMV+PWLCF+Q VKEGG  FQ 
Sbjct: 93  HHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELHKWMVDPWLCFSQGVKEGGDQFQK 152

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSV 180
           ANGLDIW FAS NPQFN  FN+AMASTSKV+MKAILSVYQDGFSS+ SL DVGGGIGG +
Sbjct: 153 ANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILSVYQDGFSSIESLTDVGGGIGGVI 212

Query: 181 SEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEE 240
           SEIVKAFPHIKGIN+DLPHV+STAP YQGVTHIGGDMFE IPKADA+FMKWILHDW+++E
Sbjct: 213 SEIVKAFPHIKGINFDLPHVISTAPPYQGVTHIGGDMFEAIPKADAVFMKWILHDWDDKE 272

Query: 241 CVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWK 300
           CVRIL NCKKAIPEKTGKVI+VEV+L  DG+GAFDDTRFYFDLLMLAHTNG+ER EKEWK
Sbjct: 273 CVRILKNCKKAIPEKTGKVILVEVILKADGDGAFDDTRFYFDLLMLAHTNGRERTEKEWK 332

Query: 301 TILEEAGFPRYRLIPLPALTSIIEAYP 328
            ILEEAGFPRYR+I LPA+TSIIEAYP
Sbjct: 333 NILEEAGFPRYRVIALPAITSIIEAYP 359

BLAST of CaUC02G042900 vs. NCBI nr
Match: KAG7034926.1 (xanthohumol 4'-O-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 571.2 bits (1471), Expect = 5.6e-159
Identity = 271/327 (82.87%), Postives = 300/327 (91.74%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL L DIINSHGSPISLSQIASSI AS+P ++P+ISYLSRIMRLLVRRNIFA 
Sbjct: 310 MALKCAVELHLADIINSHGSPISLSQIASSIVASDPLATPEISYLSRIMRLLVRRNIFAV 369

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
           HH SDGG+ LYGLTHSSKWLLRD+ LTLAPMV +ELH+WMV+PWLCF++ VKEGG  FQ 
Sbjct: 370 HHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELHKWMVDPWLCFSRGVKEGGDQFQK 429

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSV 180
           ANGLDIW FAS NPQFN  FN+AMASTSKV+MKAILSVYQDGFSS+ SL DVGGGIGG +
Sbjct: 430 ANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILSVYQDGFSSIESLTDVGGGIGGVI 489

Query: 181 SEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEE 240
           SEIVKAFPHIKG+N+DLPHV+STAP+YQGVTHIGGDMFE IPKADA+FMKWILHDW+++E
Sbjct: 490 SEIVKAFPHIKGMNFDLPHVISTAPLYQGVTHIGGDMFEAIPKADAVFMKWILHDWDDKE 549

Query: 241 CVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWK 300
           CVRIL NCKKAIPEKTGKVI+VEV+L  DG+GAFDDTRFYFDLLMLAHTNG+ER EKEWK
Sbjct: 550 CVRILKNCKKAIPEKTGKVILVEVILKADGDGAFDDTRFYFDLLMLAHTNGRERTEKEWK 609

Query: 301 TILEEAGFPRYRLIPLPALTSIIEAYP 328
            ILEEAGFPRYR+I LPA+TSIIEAYP
Sbjct: 610 NILEEAGFPRYRVIALPAITSIIEAYP 636

BLAST of CaUC02G042900 vs. ExPASy Swiss-Prot
Match: B0ZB56 (Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 2.3e-107
Identity = 190/333 (57.06%), Postives = 246/333 (73.87%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           M +KCA+EL++PDI++SH +PI+L+QIASS+     S S  +SYLSRIMRLLVRR IF+ 
Sbjct: 30  MVMKCALELRIPDIVHSHSAPITLAQIASSVP---DSPSLNLSYLSRIMRLLVRRKIFSQ 89

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPL----TLAPMVFSELHQWMVNPWLCFTQAVKEGGS 120
           H   DG + LYG THSS+ LL  + L    TLAP V    H ++  PW C  + VKEGG+
Sbjct: 90  HKSLDGEEVLYGPTHSSRLLLSKTTLPDQVTLAPFVAFMTHPYLSAPWSCLARCVKEGGN 149

Query: 121 PFQIAN-GLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGG 180
            F++ + G  +WD +  NP+FN  FND MAST+++   AILS Y+D F  + SL DVGG 
Sbjct: 150 GFEMVHGGRQLWDLSPGNPEFNKVFNDGMASTARITTMAILSEYRDVFCGICSLVDVGGE 209

Query: 181 IGGSVSEIVKAFPHIKGINYDLPHVVSTAPVYQG-VTHIGGDMFEDIPKADAIFMKWILH 240
            GGS+S IVK+ PHIKGINYDLPHVV+TAP Y G V+H+GG+MFE IP A A+FMKWILH
Sbjct: 210 FGGSISAIVKSHPHIKGINYDLPHVVATAPTYTGLVSHVGGNMFEWIPTAVAVFMKWILH 269

Query: 241 DWNEEECVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKER 300
           DW +E+CV+IL NC++A+PEK GK+IIV++VL  +G G FDD     D+ ++A T GKER
Sbjct: 270 DWADEDCVKILKNCRRAMPEKGGKIIIVDIVLEPEGNGLFDDAAVMLDIALMALTRGKER 329

Query: 301 REKEWKTILEEAGFPRYRLIPLPALTSIIEAYP 328
            EKEWK +LEE GFPRY+++ +PALTS+IEAYP
Sbjct: 330 TEKEWKRVLEEGGFPRYQILKIPALTSVIEAYP 359

BLAST of CaUC02G042900 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 4.7e-100
Identity = 178/330 (53.94%), Postives = 231/330 (70.00%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL +PDIINSHG P+++S+I  S+  + PSSSP I YL+RIMRLLV + +F +
Sbjct: 25  MALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSPNIDYLTRIMRLLVHKRLFTS 84

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
               +    LY LT SSKWLL+DS   L+P+V  E +  ++ PW    +  +E  SPF+ 
Sbjct: 85  ELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPILLKPWQYLGKCAQEKSSPFER 144

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSV-HSLADVGGGIGGS 180
           A+G +IWD A  +P+FN+F N AM  ++  ++  +L  Y+DGFS +  SL DVGGG G  
Sbjct: 145 AHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLEYKDGFSGIAGSLVDVGGGTGSI 204

Query: 181 VSEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEE 240
           ++EIVKA PHI+GIN+DLPHVV+TA  + GV H+GGDMF DIP+ADA+ MKWILHDW++E
Sbjct: 205 IAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDMFVDIPEADAVIMKWILHDWSDE 264

Query: 241 ECVRILVNCKKAI-PEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTN-GKERREK 300
           +C  IL NC +AI  +K GKVIIV+ VL  DG   FD     FD+LM+AHT  GKER E 
Sbjct: 265 DCTIILKNCYRAIRKKKNGKVIIVDCVLRPDGNDLFDKMGLIFDVLMMAHTTAGKERTEA 324

Query: 301 EWKTILEEAGFPRYRLIPLPALTSIIEAYP 328
           EWK +L  AGFPRY +I  PA   IIEA+P
Sbjct: 325 EWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

BLAST of CaUC02G042900 vs. ExPASy Swiss-Prot
Match: B0ZB55 (Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 PE=1 SV=2)

HSP 1 Score: 344.0 bits (881), Expect = 1.9e-93
Identity = 172/330 (52.12%), Postives = 234/330 (70.91%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAA-SNPSSSPQISYLSRIMRLLVRRNIFA 60
           MALKCA+EL++ DII+SHG PI+LSQIAS I + SN S SP I YLSRIMR LVR+NIF 
Sbjct: 21  MALKCAIELRIADIIHSHGKPITLSQIASGIRSNSNSSISPNIPYLSRIMRFLVRKNIFT 80

Query: 61  AH-HPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPF 120
            H   +D   +LYGL+ SS+WLLRD   +LAPMV  + H   +  W      V+   + F
Sbjct: 81  EHQEDNDEVISLYGLSDSSRWLLRDFKSSLAPMVLMQTHPLSMAVWHFLEDYVRNSSNTF 140

Query: 121 QIANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVH-SLADVGGGIG 180
           + A+G +IW+F+S NP FN  FN+AMAS   + M A+LS Y+DG   +  ++ DVGGG G
Sbjct: 141 EKAHGCNIWEFSSANPDFNKIFNNAMASIVPIYMGAVLSSYKDGLGCIKGTVVDVGGGTG 200

Query: 181 GSVSEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWN 240
           GS+SE++K +P+IKGIN+DLPHV++TAP   GVTHI GD+FE IP ADA+ MK +LH ++
Sbjct: 201 GSISELMKYYPNIKGINFDLPHVIATAPALDGVTHISGDIFESIPSADAVLMKGVLHCFS 260

Query: 241 EEECVRILVNCKKAIPE-KTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERRE 300
           +E+CV++L NC+KAI + K GK+II+E+VL+      FD+TR  +DLL+   + GKER E
Sbjct: 261 DEKCVKVLRNCRKAITDKKNGKIIILEIVLDPTSNQIFDETRMVYDLLIPLFSGGKERTE 320

Query: 301 KEWKTILEEAGFPRYRLIPLPALTSIIEAY 327
            EWK +L EAGF   ++  +P + +IIEA+
Sbjct: 321 LEWKRLLNEAGFTSIKITKIPIIPAIIEAF 350

BLAST of CaUC02G042900 vs. ExPASy Swiss-Prot
Match: B0ZB57 (Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.0e-70
Identity = 141/338 (41.72%), Postives = 209/338 (61.83%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFA- 60
           M+LKCA++L +PDIIN+HG P+++SQ+  ++   N   SP +    R+MR+L+    FA 
Sbjct: 44  MSLKCAIQLGIPDIINNHGKPMTISQLTLALPI-NRKKSPCV---YRLMRILIHSGFFAL 103

Query: 61  --AHHPSDGG--DTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGG 120
             A    +GG  +  Y +T +SK LL+D+P+++ P + + L   M  PW   +   + G 
Sbjct: 104 QKAEVGEEGGGEEEGYVITDASKLLLKDNPMSVTPFLLAMLDPVMTKPWDFLSNWFQNGD 163

Query: 121 -SPFQIANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGG 180
            +PF  ANG+  WD+ S  P+   FFNDAMAS +++V   ++   +  F  V SL DVGG
Sbjct: 164 PTPFDTANGMAFWDYGSHEPKLARFFNDAMASDARLVTSVVIEKCKGVFEGVESLVDVGG 223

Query: 181 GIGGSVSEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILH 240
           G G   S I  AFPHI+   +DLPHVV+       +  +GGDMF D+P  + + +KWILH
Sbjct: 224 GTGTVASSIAAAFPHIQCTVFDLPHVVADLQGGNNLNFVGGDMFVDVPATEVVLLKWILH 283

Query: 241 DWNEEECVRILVNCKKAIP---EKTGKVIIVEV-VLNE-DGEGAFDDTRFYFDLLMLAHT 300
           DWN+EE V+IL  CK+AI    +K GKVII+++ V NE D +    +T+ +FD+LM+A  
Sbjct: 284 DWNDEESVKILKKCKEAISKSNKKGGKVIIIDMKVENEKDEDDESYETQLFFDMLMMALV 343

Query: 301 NGKERREKEWKTILEEAGFPRYRLIPLPALTSIIEAYP 328
            G+ER EKEW  + ++AGF  Y++ P+  L S+IE YP
Sbjct: 344 TGRERNEKEWAKLFKDAGFSNYKITPILGLRSLIEVYP 377

BLAST of CaUC02G042900 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.4e-67
Identity = 132/329 (40.12%), Postives = 204/329 (62.01%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           + LKCAV+L L +II++ G+ ++LS+++S +    PS       L R+MR LV   +F  
Sbjct: 25  LVLKCAVQLDLANIIHNSGTSMTLSELSSRL----PSQPVNEDALYRVMRYLVHMKLFT- 84

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVK-EGGSPFQ 120
              S  G+  YGL   +K+L++     +   + +   +  + PW      +  E G+ F+
Sbjct: 85  -KASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFMAPWHYLKDGLSGESGTAFE 144

Query: 121 IANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGS 180
            A G +IW + + +P+ N  FN+AMA+ S+++M A++    + F+ + +L DVGGG G +
Sbjct: 145 KALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVKECGNIFNGITTLVDVGGGTGTA 204

Query: 181 VSEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEE 240
           V  I  AFPHIK   YDLPHV++ +P Y  V  + GDMF+ IPKADAI MK ILHDW+++
Sbjct: 205 VRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFKFIPKADAIMMKCILHDWDDK 264

Query: 241 ECVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEW 300
           EC+ IL  CK+A+P K GKVIIV++VLN   E  +   R   DL M+ +T GKER E+EW
Sbjct: 265 ECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRLTLDLDMMLNTGGKERTEEEW 324

Query: 301 KTILEEAGFPRYRLIPLPALTSIIEAYPF 329
           K ++ +AG+  +++  + A+ S+IEAYP+
Sbjct: 325 KKLIHDAGYKGHKITQITAVQSVIEAYPY 347

BLAST of CaUC02G042900 vs. ExPASy TrEMBL
Match: A0A5A7V8T5 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G001350 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 8.7e-174
Identity = 297/327 (90.83%), Postives = 315/327 (96.33%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL L DIINSHGSPISLSQI+SSIAASNPSSSPQISYL+RIMRLLVRRNIFAA
Sbjct: 30  MALKCAVELHLADIINSHGSPISLSQISSSIAASNPSSSPQISYLNRIMRLLVRRNIFAA 89

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
           HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM FSELHQWMV+PWLCFT+AVKEGGSPF+I
Sbjct: 90  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMAFSELHQWMVSPWLCFTEAVKEGGSPFKI 149

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSV 180
           A+GLDIWDFAS+NPQFNHFFNDAMASTSKVVM AILSVYQDGF+S+ SLADVGGGIGGS+
Sbjct: 150 AHGLDIWDFASKNPQFNHFFNDAMASTSKVVMNAILSVYQDGFNSLDSLADVGGGIGGSI 209

Query: 181 SEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEE 240
           SEIVKAFPHIKGINYDLPHV+STAPVY+GVTHIGGDMFEDIPK DAIFMKWILHDWN++E
Sbjct: 210 SEIVKAFPHIKGINYDLPHVISTAPVYEGVTHIGGDMFEDIPKVDAIFMKWILHDWNDKE 269

Query: 241 CVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWK 300
           CV+IL NCKKAIPEK GKVIIVEVVLNE+G+GAFDDT+FYFDLLMLAHTNGKER EKEWK
Sbjct: 270 CVKILENCKKAIPEKRGKVIIVEVVLNEEGKGAFDDTKFYFDLLMLAHTNGKERTEKEWK 329

Query: 301 TILEEAGFPRYRLIPLPALTSIIEAYP 328
           TILEEAGF RY LIPLPAL SIIEAYP
Sbjct: 330 TILEEAGFSRYNLIPLPALVSIIEAYP 356

BLAST of CaUC02G042900 vs. ExPASy TrEMBL
Match: A0A1S3BJA7 ((R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490512 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 8.7e-174
Identity = 297/327 (90.83%), Postives = 315/327 (96.33%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL L DIINSHGSPISLSQI+SSIAASNPSSSPQISYL+RIMRLLVRRNIFAA
Sbjct: 30  MALKCAVELHLADIINSHGSPISLSQISSSIAASNPSSSPQISYLNRIMRLLVRRNIFAA 89

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
           HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPM FSELHQWMV+PWLCFT+AVKEGGSPF+I
Sbjct: 90  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMAFSELHQWMVSPWLCFTEAVKEGGSPFKI 149

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSV 180
           A+GLDIWDFAS+NPQFNHFFNDAMASTSKVVM AILSVYQDGF+S+ SLADVGGGIGGS+
Sbjct: 150 AHGLDIWDFASKNPQFNHFFNDAMASTSKVVMNAILSVYQDGFNSLDSLADVGGGIGGSI 209

Query: 181 SEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEE 240
           SEIVKAFPHIKGINYDLPHV+STAPVY+GVTHIGGDMFEDIPK DAIFMKWILHDWN++E
Sbjct: 210 SEIVKAFPHIKGINYDLPHVISTAPVYEGVTHIGGDMFEDIPKVDAIFMKWILHDWNDKE 269

Query: 241 CVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWK 300
           CV+IL NCKKAIPEK GKVIIVEVVLNE+G+GAFDDT+FYFDLLMLAHTNGKER EKEWK
Sbjct: 270 CVKILENCKKAIPEKRGKVIIVEVVLNEEGKGAFDDTKFYFDLLMLAHTNGKERTEKEWK 329

Query: 301 TILEEAGFPRYRLIPLPALTSIIEAYP 328
           TILEEAGF RY LIPLPAL SIIEAYP
Sbjct: 330 TILEEAGFSRYNLIPLPALVSIIEAYP 356

BLAST of CaUC02G042900 vs. ExPASy TrEMBL
Match: A0A0A0KAJ3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G128650 PE=3 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 1.6e-172
Identity = 296/327 (90.52%), Postives = 312/327 (95.41%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL L DIINSHGSPISLSQIASSIAASNPSS+PQISYL+RIMRLLVRRNIFAA
Sbjct: 30  MALKCAVELHLADIINSHGSPISLSQIASSIAASNPSSAPQISYLNRIMRLLVRRNIFAA 89

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
           HHPSDGGD LYGLTHSSKWLLRDSPLTLAPM FSELHQWMVNPWLCFT AVKEGGSPF+I
Sbjct: 90  HHPSDGGDALYGLTHSSKWLLRDSPLTLAPMAFSELHQWMVNPWLCFTGAVKEGGSPFKI 149

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSV 180
           ANGLDIWDFA +NPQFNHFFN+AMASTSKVVM A+LSVY DGF+SVHSLADVGGGIGGS+
Sbjct: 150 ANGLDIWDFAFKNPQFNHFFNNAMASTSKVVMNAVLSVYLDGFNSVHSLADVGGGIGGSI 209

Query: 181 SEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEE 240
           SEIVKA PHIKGINYDLPHVVSTAPVY+GV HIGGDMFEDIPKADAIFMKWILHDWN++E
Sbjct: 210 SEIVKALPHIKGINYDLPHVVSTAPVYEGVIHIGGDMFEDIPKADAIFMKWILHDWNDKE 269

Query: 241 CVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWK 300
           CV+IL NCKKAIPEK GKVIIV+VVLNE+G+GAFDDTRFYFDLLMLAHTNGKER EKEWK
Sbjct: 270 CVKILENCKKAIPEKRGKVIIVDVVLNEEGKGAFDDTRFYFDLLMLAHTNGKERTEKEWK 329

Query: 301 TILEEAGFPRYRLIPLPALTSIIEAYP 328
           TILEEAGF RY+LIPLPAL SIIEAYP
Sbjct: 330 TILEEAGFSRYKLIPLPALVSIIEAYP 356

BLAST of CaUC02G042900 vs. ExPASy TrEMBL
Match: A0A6J1G506 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111450978 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.3e-160
Identity = 273/327 (83.49%), Postives = 299/327 (91.44%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL L DIINSHGSPISLSQIASSI AS+P ++P+ISYLSRIMRLLVRRNIFA 
Sbjct: 33  MALKCAVELHLADIINSHGSPISLSQIASSIVASDPLATPEISYLSRIMRLLVRRNIFAV 92

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
           HH SDGG+ LYGLTHSSKWLLRD+ LTLAPMV +ELH+WMV+PWLCF+Q VKEGG  FQ 
Sbjct: 93  HHSSDGGEPLYGLTHSSKWLLRDAELTLAPMVLAELHKWMVDPWLCFSQGVKEGGDQFQK 152

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSV 180
           ANGLDIW FAS NPQFN  FN+AMASTSKV+MKAILSVYQDGFSS+ SL DVGGGIGG +
Sbjct: 153 ANGLDIWSFASTNPQFNQLFNNAMASTSKVIMKAILSVYQDGFSSIESLTDVGGGIGGVI 212

Query: 181 SEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEE 240
           SEIVKAFPHIKGIN+DLPHV+STAP YQGVTHIGGDMFE IPKADA+FMKWILHDW+++E
Sbjct: 213 SEIVKAFPHIKGINFDLPHVISTAPPYQGVTHIGGDMFEAIPKADAVFMKWILHDWDDKE 272

Query: 241 CVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWK 300
           CVRIL NCKKAIPEKTGKVI+VEV+L  DG+GAFDDTRFYFDLLMLAHTNG+ER EKEWK
Sbjct: 273 CVRILKNCKKAIPEKTGKVILVEVILKADGDGAFDDTRFYFDLLMLAHTNGRERTEKEWK 332

Query: 301 TILEEAGFPRYRLIPLPALTSIIEAYP 328
            ILEEAGFPRYR+I LPA+TSIIEAYP
Sbjct: 333 NILEEAGFPRYRVIALPAITSIIEAYP 359

BLAST of CaUC02G042900 vs. ExPASy TrEMBL
Match: A0A6J1I4R1 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111469616 PE=3 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 8.7e-158
Identity = 269/327 (82.26%), Postives = 296/327 (90.52%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALKCAVEL L DIINSH SP+SLSQIASSI AS+P ++P+ISYL+RIMRLLVRRNIFA 
Sbjct: 30  MALKCAVELHLADIINSHASPLSLSQIASSIVASDPLATPEISYLTRIMRLLVRRNIFAV 89

Query: 61  HHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQI 120
           HH  DGG+ LYGLTHSSKWLLRD+  TLAPMV +ELH+WMV+PWLCF+Q VKEGG  FQ 
Sbjct: 90  HHSPDGGEPLYGLTHSSKWLLRDAEHTLAPMVLAELHKWMVDPWLCFSQGVKEGGDQFQK 149

Query: 121 ANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGSV 180
           ANGLDIW FASRNPQFN  FN+AMASTSKV+MKA+LSVYQDGFSS+ SL DVGGGIGG +
Sbjct: 150 ANGLDIWSFASRNPQFNQLFNNAMASTSKVIMKAVLSVYQDGFSSIESLTDVGGGIGGVI 209

Query: 181 SEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEEE 240
           SEIVKAFPHIKGINYDLPHV+STAP YQGVTHIGGDMFE IPKADA+FMKWILHDW+++E
Sbjct: 210 SEIVKAFPHIKGINYDLPHVISTAPPYQGVTHIGGDMFEAIPKADAVFMKWILHDWDDKE 269

Query: 241 CVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTRFYFDLLMLAHTNGKERREKEWK 300
           CVRIL NCKKAIPEKTGKVI+VEV+L  DG+G FDDTRFYFDLLMLAHTNG+ER EKEWK
Sbjct: 270 CVRILENCKKAIPEKTGKVILVEVILKADGDGPFDDTRFYFDLLMLAHTNGRERTEKEWK 329

Query: 301 TILEEAGFPRYRLIPLPALTSIIEAYP 328
            ILEEAGFPRYR+I LPALTSIIEAYP
Sbjct: 330 NILEEAGFPRYRVIALPALTSIIEAYP 356

BLAST of CaUC02G042900 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.1e-67
Identity = 139/343 (40.52%), Postives = 203/343 (59.18%), Query Frame = 0

Query: 2   ALKCAVELQLPDIINSHGS--PISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFA 61
           A KCA++L++P+ I +H S  P++L++++S+++AS        S+L RIMR LV + IF 
Sbjct: 45  AAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSASP-------SHLRRIMRFLVHQGIFK 104

Query: 62  AHHPSDGGDTLYGLTHSSKWLL--RDSPLTLAPMVFSELHQWMVNPWLCFTQAVKE--GG 121
                DG  T Y  T  S+ L+  R    +LAP V  E    M+ PWL  +  V     G
Sbjct: 105 EIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPEMLAPWLRLSSVVSSPVNG 164

Query: 122 S---PFQIANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADV 181
           S   PF   +G D+W FA  NP  +   N+AMA  ++ V+  +       F  V ++ DV
Sbjct: 165 STPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRVVPRVAGACHGLFDGVTTMVDV 224

Query: 182 GGGIGGSVSEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWI 241
           GGG G ++  +VK FP IKG N+DLPHV+  A V  GV ++ GDMF+ IP  DAIF+KW+
Sbjct: 225 GGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENVEGDMFDSIPACDAIFIKWV 284

Query: 242 LHDWNEEECVRILVNCKKAIPEKTGKVIIVEVVLNE--------DGEGAFDDTRFYFDLL 301
           LHDW +++C++IL NCK+A+P   GKV+IVE V+ E        + +   +  R   D++
Sbjct: 285 LHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTMIVDERDEKLEHVRLMLDMV 344

Query: 302 MLAHTN-GKERREKEWKTILEEAGFPRYRLIPLPALTSIIEAY 327
           M+AHT+ GKER  KEW  +L+EAGF RY +  +  + S+I AY
Sbjct: 345 MMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLIIAY 380

BLAST of CaUC02G042900 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 243.8 bits (621), Expect = 1.9e-64
Identity = 126/320 (39.38%), Postives = 197/320 (61.56%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFAA 60
           MALK A+EL L +I+  +GSP+S ++IAS +   NP +      L RI+RLL   ++   
Sbjct: 34  MALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVM---LDRILRLLTSYSVLTC 93

Query: 61  HH---PSDGGDTLYGLTHSSKWLLR-DSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGS 120
            +     DG + +YGL    K+L + +  +++A +      + ++  W     A+ +GG 
Sbjct: 94  SNRKLSGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGGI 153

Query: 121 PFQIANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGI 180
           PF  A G+  +++   +P+FN  FN+ M++ S + MK IL  Y+ GF  + SL DVGGGI
Sbjct: 154 PFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYK-GFEGLTSLVDVGGGI 213

Query: 181 GGSVSEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDW 240
           G ++  IV  +P++KGIN+DLPHV+  AP + G+ H+GGDMF  +PK DAIFMKWI HDW
Sbjct: 214 GATLKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDW 273

Query: 241 NEEECVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTR-FYFDLLMLAHT-NGKER 300
           ++E CV+ L NC +++PE  GKVI+ E +L E  + +    +  + D +MLAH   GKER
Sbjct: 274 SDEHCVKFLKNCYESLPE-DGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKER 333

Query: 301 REKEWKTILEEAGFPRYRLI 315
            EKE++ + + +GF   +++
Sbjct: 334 TEKEFEALAKASGFKGIKVV 348

BLAST of CaUC02G042900 vs. TAIR 10
Match: AT1G51990.1 (O-methyltransferase family protein )

HSP 1 Score: 215.3 bits (547), Expect = 7.3e-56
Identity = 131/336 (38.99%), Postives = 198/336 (58.93%), Query Frame = 0

Query: 3   LKCAVELQLPDII---NSHGSPISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFA 62
           +K A EL L +I+      GS +S   +AS  A  NP +   I    R++R LV  ++  
Sbjct: 32  VKTARELDLFEIMAKARPLGSYLSPVDLASMAAPKNPHAPMMI---DRLLRFLVAYSVCT 91

Query: 63  AHHPSD--GGDT-LYGLTHSSKWLLRDSP-LTLAPMVFSELHQWMVNPWLCFTQAVKEGG 122
                D  G ++  YGL    K L++D    ++AP V +   +     W   T+A++EGG
Sbjct: 92  CKLVKDEEGRESRAYGLGKVGKKLIKDEDGFSIAPYVLAGCTKAKGGVWSYLTEAIQEGG 151

Query: 123 -SPFQIANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGG 182
            S ++ AN   I+++  +N      FN++M + + +VMK IL  Y  GF  V    DVGG
Sbjct: 152 ASAWERANEALIFEYMKKNENLKKIFNESMTNHTSIVMKKILENY-IGFEGVSDFVDVGG 211

Query: 183 GIGGSVSEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILH 242
            +G ++++I+  +PHIKGIN+DLPH+V  AP   GV HIGGDMF++IP+ + I MKWILH
Sbjct: 212 SLGSNLAQILSKYPHIKGINFDLPHIVKEAPQIHGVEHIGGDMFDEIPRGEVILMKWILH 271

Query: 243 DWNEEECVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGAFDDTR--FYFDLLMLAHTN-G 302
           DWN+E+CV IL NCKKA+PE TG++I++E+++  +       T+     DL M++ T+ G
Sbjct: 272 DWNDEKCVEILKNCKKALPE-TGRIIVIEMIVPREVSETDLATKNSLSADLTMMSLTSGG 331

Query: 303 KERREKEWKTILEEAGFPRYRLIPLPALTSIIEAYP 328
           KER +KE++ + +EAGF   ++I       IIE YP
Sbjct: 332 KERTKKEFEDLAKEAGFKLPKIIYGAYSYWIIELYP 362

BLAST of CaUC02G042900 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.6e-55
Identity = 132/339 (38.94%), Postives = 192/339 (56.64%), Query Frame = 0

Query: 1   MALKCAVELQLPDIINSHGSPISLS--QIASSIAASNPSSSPQISYLSRIMRLLVRRNIF 60
           M LK A EL + D I + G+   LS  +IA S+  + P++      L R++ LLV  +I 
Sbjct: 44  MVLKAAFELGVIDTIAAAGNDTWLSPCEIACSL-PTKPTNPEAPVLLDRMLSLLVSHSIL 103

Query: 61  AAHHPSDGG-------DTLYGLTHSSKWLLRDSPLT--LAPMVFSELH-QWMVNPWLCFT 120
                  G        + +Y      K+ LRDS  T  L P+ F  LH Q     W    
Sbjct: 104 KCRMIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPL-FMLLHTQVFFKTWTNLK 163

Query: 121 QAVKEGGSPFQIANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHS 180
             + EG   F  A+G+ I+++ + +  F   FN AM+  S ++MK +L VY+ GF  V++
Sbjct: 164 DVILEGRDAFNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVLDVYR-GFEDVNT 223

Query: 181 LADVGGGIGGSVSEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIF 240
           L DVGGG G  +  +   +PHIKG+N+DL  V++ AP Y GV H+ GDMF ++PK DA+F
Sbjct: 224 LVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDAVF 283

Query: 241 MKWILHDWNEEECVRILVNCKKAIPEKTGKVIIVEVVLNEDGEGA--FDDTRFYFDLLML 300
           MKWILHDW +E+C++IL NC K++PEK GK+IIVE V  ++ +G     +T F  DLLML
Sbjct: 284 MKWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEFVTPKEPKGGDLSSNTVFAMDLLML 343

Query: 301 AH-TNGKERREKEWKTILEEAGFPRYRLIPLPALTSIIE 325
              + GKER   +++ +   +GF R  +I L    S+IE
Sbjct: 344 TQCSGGKERSLSQFENLAFASGFLRCEIICLAYSYSVIE 378

BLAST of CaUC02G042900 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 213.0 bits (541), Expect = 3.6e-55
Identity = 122/336 (36.31%), Postives = 184/336 (54.76%), Query Frame = 0

Query: 2   ALKCAVELQLPDIINSHGS--PISLSQIASSIAASNPSSSPQISYLSRIMRLLVRRNIFA 61
           A KCA++L++P+ I +H S  P++LS+++S+++AS        S+L RIMR LV + +F 
Sbjct: 31  AAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASP-------SHLRRIMRFLVHQGLFK 90

Query: 62  AHHPSDGGDTLYGLTHSSKWLLRDSPLTLAPMVFSELHQWMVNPWLCFTQAVKEGGSPFQ 121
                DG  T Y  T  S+            M+ ++LH                      
Sbjct: 91  EVPTKDGLATGYTNTPLSR-----------RMMITKLH---------------------- 150

Query: 122 IANGLDIWDFASRNPQFNHFFNDAMASTSKVVMKAILSVYQDGFSSVHSLADVGGGIGGS 181
              G D+W FA  N   +   N+AMA  ++ V+  +    Q  F  V ++ DVGGG G +
Sbjct: 151 ---GKDLWAFAQDNLCHSQLINEAMACDARRVVPRVAGACQGLFDGVATVVDVGGGTGET 210

Query: 182 VSEIVKAFPHIKGINYDLPHVVSTAPVYQGVTHIGGDMFEDIPKADAIFMKWILHDWNEE 241
           +  +VK FP IKG N+DLPHV+  A V  GV ++ GDMF+ IP +DA+ +KW+LHDW ++
Sbjct: 211 MGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPASDAVIIKWVLHDWGDK 270

Query: 242 ECVRILVNCKKAIPEKTGKVIIVEVVLN--------EDGEGAFDDTRFYFDLLMLAHTN- 301
           +C++IL NCK+A+    GKV+IVE V+         E+ +   +  R   D++M+ HT+ 
Sbjct: 271 DCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDKLEHVRLQLDMVMMVHTST 323

Query: 302 GKERREKEWKTILEEAGFPRYRLIPLPALTSIIEAY 327
           GKER  KEW  +L EAGF RY +     + S+I AY
Sbjct: 331 GKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIAY 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902222.16.0e-17792.66xanthohumol 4-O-methyltransferase-like [Benincasa hispida][more]
XP_008448276.21.8e-17390.83PREDICTED: (R,S)-reticuline 7-O-methyltransferase-like [Cucumis melo] >KAA006210... [more]
XP_004139935.13.4e-17290.52xanthohumol 4-O-methyltransferase [Cucumis sativus] >KGN46755.1 hypothetical pro... [more]
XP_022946951.11.9e-15983.49xanthohumol 4'-O-methyltransferase-like [Cucurbita moschata][more]
KAG7034926.15.6e-15982.87xanthohumol 4'-O-methyltransferase, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
B0ZB562.3e-10757.06Xanthohumol 4-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT2 PE=1 SV=1[more]
Q6WUC24.7e-10053.94(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=... [more]
B0ZB551.9e-9352.12Desmethylxanthohumol 6'-O-methyltransferase OS=Humulus lupulus OX=3486 GN=OMT1 P... [more]
B0ZB571.0e-7041.72Probable O-methyltransferase 3 OS=Humulus lupulus OX=3486 GN=OMT3 PE=2 SV=1[more]
Q9LEL61.4e-6740.12(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7V8T58.7e-17490.83(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3BJA78.7e-17490.83(R,S)-reticuline 7-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490... [more]
A0A0A0KAJ31.6e-17290.52Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G128650 PE=3 SV=1[more]
A0A6J1G5069.3e-16083.49xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I4R18.7e-15882.26xanthohumol 4'-O-methyltransferase-like OS=Cucurbita maxima OX=3661 GN=LOC111469... [more]
Match NameE-valueIdentityDescription
AT4G35160.14.1e-6740.52O-methyltransferase family protein [more]
AT5G54160.11.9e-6439.38O-methyltransferase 1 [more]
AT1G51990.17.3e-5638.99O-methyltransferase family protein [more]
AT1G77520.13.6e-5538.94O-methyltransferase family protein [more]
AT4G35150.13.6e-5536.31O-methyltransferase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001077O-methyltransferase domainPFAMPF00891Methyltransf_2coord: 105..308
e-value: 2.9E-63
score: 213.0
IPR016461O-methyltransferase COMT-typePIRSFPIRSF005739O-mtasecoord: 1..328
e-value: 3.3E-121
score: 402.5
IPR016461O-methyltransferase COMT-typePANTHERPTHR11746O-METHYLTRANSFERASEcoord: 1..327
IPR016461O-methyltransferase COMT-typePROSITEPS51683SAM_OMT_IIcoord: 1..327
score: 77.88372
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 83..328
e-value: 2.0E-82
score: 278.1
NoneNo IPR availablePIRSRPIRSR005739-1PIRSR005739-1coord: 1..326
e-value: 2.4E-94
score: 313.5
NoneNo IPR availablePANTHERPTHR11746:SF235(R,S)-RETICULINE 7-O-METHYLTRANSFERASE-LIKE ISOFORM X1coord: 1..327
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 1..80
e-value: 1.0E-25
score: 91.4
IPR012967Plant methyltransferase dimerisationPFAMPF08100Dimerisationcoord: 1..53
e-value: 1.4E-15
score: 57.0
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 1..85
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 85..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC02G042900.1CaUC02G042900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019438 aromatic compound biosynthetic process
biological_process GO:0032259 methylation
molecular_function GO:0050630 (iso)eugenol O-methyltransferase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0102719 S-adenosyl-L-methionine:eugenol-O-methyltransferase activity
molecular_function GO:0008757 S-adenosylmethionine-dependent methyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0008171 O-methyltransferase activity