CaUC01G000570 (gene) Watermelon (USVL246-FR2) v1

Overview
NameCaUC01G000570
Typegene
OrganismCitrullus amarus (Watermelon (USVL246-FR2) v1)
DescriptionSuccinate dehydrogenase assembly factor 4, mitochondrial
LocationCiama_Chr01: 419290 .. 419625 (+)
RNA-Seq ExpressionCaUC01G000570
SyntenyCaUC01G000570
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGCAATCTCAGCCGTCTATTCTCGTCGCCGCCGACTCTCTCCGCGCTACCGGCGGCGAGTCGCCTGACTGCCAACTCGTTGAGCCGGCTCGTTAGCTCCTCGTCCAATCAACAACCTTCTCGATCGGAGCAAGGTGTGTCTAGCGAGGAACAACGAGAGGCCGGGAAAGGAAGTTTGCCGAATCAACACGAGGCTGCCATAGATGACCGAGACGGTGAAGATGAAGGAGTTGATATAAAAGAAGCGACCGGCGAGGTCGGTGGACCTAAAGGCCCCGAGCCCACCCGCTACGGCGATTGGGAACGAAACGGTCGGTGCTACGATTTCTGA

mRNA sequence

ATGTCGAGCAATCTCAGCCGTCTATTCTCGTCGCCGCCGACTCTCTCCGCGCTACCGGCGGCGAGTCGCCTGACTGCCAACTCGTTGAGCCGGCTCGTTAGCTCCTCGTCCAATCAACAACCTTCTCGATCGGAGCAAGGTGTGTCTAGCGAGGAACAACGAGAGGCCGGGAAAGGAAGTTTGCCGAATCAACACGAGGCTGCCATAGATGACCGAGACGGTGAAGATGAAGGAGTTGATATAAAAGAAGCGACCGGCGAGGTCGGTGGACCTAAAGGCCCCGAGCCCACCCGCTACGGCGATTGGGAACGAAACGGTCGGTGCTACGATTTCTGA

Coding sequence (CDS)

ATGTCGAGCAATCTCAGCCGTCTATTCTCGTCGCCGCCGACTCTCTCCGCGCTACCGGCGGCGAGTCGCCTGACTGCCAACTCGTTGAGCCGGCTCGTTAGCTCCTCGTCCAATCAACAACCTTCTCGATCGGAGCAAGGTGTGTCTAGCGAGGAACAACGAGAGGCCGGGAAAGGAAGTTTGCCGAATCAACACGAGGCTGCCATAGATGACCGAGACGGTGAAGATGAAGGAGTTGATATAAAAGAAGCGACCGGCGAGGTCGGTGGACCTAAAGGCCCCGAGCCCACCCGCTACGGCGATTGGGAACGAAACGGTCGGTGCTACGATTTCTGA

Protein sequence

MSSNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGSLPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF
Homology
BLAST of CaUC01G000570 vs. NCBI nr
Match: KGN49871.1 (hypothetical protein Csa_000062 [Cucumis sativus])

HSP 1 Score: 173.3 bits (438), Expect = 1.2e-39
Identity = 93/111 (83.78%), Postives = 99/111 (89.19%), Query Frame = 0

Query: 1   MSSNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGS 60
           MSSNLSRLFSSPPTLSALPA SRLT NSLSR VSSSSNQQPSR E+GVSSEEQREAGKGS
Sbjct: 1   MSSNLSRLFSSPPTLSALPAVSRLTFNSLSRFVSSSSNQQPSRLEKGVSSEEQREAGKGS 60

Query: 61  LPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF 112
            PN+HEAA DDR G+DEGVD ++ T E+GGPKGPEPTRYGDWER GRC DF
Sbjct: 61  SPNRHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERKGRCSDF 111

BLAST of CaUC01G000570 vs. NCBI nr
Match: KAA0042555.1 (succinate dehydrogenase assembly factor 4 [Cucumis melo var. makuwa] >TYK05959.1 succinate dehydrogenase assembly factor 4 [Cucumis melo var. makuwa])

HSP 1 Score: 168.7 bits (426), Expect = 2.8e-38
Identity = 90/111 (81.08%), Postives = 97/111 (87.39%), Query Frame = 0

Query: 1   MSSNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGS 60
           MSS+LSRLFSSPPTLSALP   RLT+NSLSR VSSSSNQQPSRSEQGVSSEEQREA KGS
Sbjct: 1   MSSHLSRLFSSPPTLSALPTVGRLTSNSLSRFVSSSSNQQPSRSEQGVSSEEQREAEKGS 60

Query: 61  LPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF 112
            PNQHEAA DDR  +D+GVD ++   E+GGPKG EPTRYGDWERNGRCYDF
Sbjct: 61  SPNQHEAAGDDRGSQDQGVDTEKMAPEIGGPKGHEPTRYGDWERNGRCYDF 111

BLAST of CaUC01G000570 vs. NCBI nr
Match: XP_022995844.1 (succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita maxima])

HSP 1 Score: 168.7 bits (426), Expect = 2.8e-38
Identity = 89/111 (80.18%), Postives = 100/111 (90.09%), Query Frame = 0

Query: 1   MSSNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGS 60
           MSSNL+RLF SPP+L+AL A +R+T+ S+SRLVSSS+NQQPSR EQGV  EEQREAGKGS
Sbjct: 1   MSSNLTRLF-SPPSLTALSAVNRVTSTSVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  LPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF 112
           LP+QHEAA DDRD EDEG D+ +ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of CaUC01G000570 vs. NCBI nr
Match: XP_022957978.1 (succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita moschata])

HSP 1 Score: 166.8 bits (421), Expect = 1.1e-37
Identity = 89/111 (80.18%), Postives = 99/111 (89.19%), Query Frame = 0

Query: 1   MSSNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGS 60
           MSSNL+RLF SPP+L+AL A +R T+ S+SRLVSSS+NQQPSR EQGV  EEQREAGKGS
Sbjct: 1   MSSNLTRLF-SPPSLTALSAVNRATSISVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  LPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF 112
           LP+QHEAA DDRD EDEG D+ +ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of CaUC01G000570 vs. NCBI nr
Match: XP_023532848.1 (succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 165.6 bits (418), Expect = 2.4e-37
Identity = 88/111 (79.28%), Postives = 99/111 (89.19%), Query Frame = 0

Query: 1   MSSNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGS 60
           MSSNL+RLF SPP+L++L A +R T+ S+SRLVSSS+NQQPSR EQGV  EEQREAGKGS
Sbjct: 1   MSSNLTRLF-SPPSLTSLSAVNRATSISVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  LPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF 112
           LP+QHEAA DDRD EDEG D+ +ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of CaUC01G000570 vs. ExPASy Swiss-Prot
Match: Q8BTE0 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Mus musculus OX=10090 GN=Sdhaf4 PE=3 SV=2)

HSP 1 Score: 55.1 bits (131), Expect = 6.0e-07
Identity = 31/72 (43.06%), Postives = 40/72 (55.56%), Query Frame = 0

Query: 45  EQGVSSEEQREAGKGSLPNQHEAAIDDRDGEDEGV-----DIKEATGEVGGPKGPEPTRY 104
           ++G     ++   K  LP     +++D   E E +     D+   T E GGPKGPEPTRY
Sbjct: 33  QEGKPEPAKQALKKSKLPLGRFDSLEDSPEEREPLQKFPDDVNPVTKEKGGPKGPEPTRY 92

Query: 105 GDWERNGRCYDF 112
           GDWER GRC DF
Sbjct: 93  GDWERKGRCIDF 104

BLAST of CaUC01G000570 vs. ExPASy Swiss-Prot
Match: Q9VLU6 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Drosophila melanogaster OX=7227 GN=Sirup PE=3 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 7.8e-07
Identity = 22/27 (81.48%), Postives = 23/27 (85.19%), Query Frame = 0

Query: 85  TGEVGGPKGPEPTRYGDWERNGRCYDF 112
           TGE+GGP GPEPTRYGDWER GR  DF
Sbjct: 92  TGEIGGPAGPEPTRYGDWERKGRVSDF 118

BLAST of CaUC01G000570 vs. ExPASy Swiss-Prot
Match: Q5VUM1 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Homo sapiens OX=9606 GN=SDHAF4 PE=1 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.0e-06
Identity = 35/80 (43.75%), Postives = 44/80 (55.00%), Query Frame = 0

Query: 37  SNQQPSRSEQGVSSEEQREAGKGSLPNQHEAAIDDRDGEDEGV-----DIKEATGEVGGP 96
           S ++ S S+ G S   ++   K  LP     A +D   E E +     D+   T E GGP
Sbjct: 29  SLRKTSSSQGGKSELVKQSLKKPKLPEGRFDAPEDSHLEKEPLEKFPDDVNPVTKEKGGP 88

Query: 97  KGPEPTRYGDWERNGRCYDF 112
           +GPEPTRYGDWER GRC DF
Sbjct: 89  RGPEPTRYGDWERKGRCIDF 108

BLAST of CaUC01G000570 vs. ExPASy Swiss-Prot
Match: Q54Y25 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=DDB_G0278459 PE=3 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 1.5e-05
Identity = 34/113 (30.09%), Postives = 60/113 (53.10%), Query Frame = 0

Query: 3   SNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGSLP 62
           +NL   F+    L+A    +++  +  S  +++++N   + +   +S++      K  + 
Sbjct: 2   NNLISKFNKIIYLNATKQTNKINCSFYSTTINNNNNNNNNNNNNNISNK------KAEMS 61

Query: 63  NQHEAAIDDRDGEDEGVDIK----EATGEVGGPKGPEPTRYGDWERNGRCYDF 112
            +++  + D + E +  + K      T E+GGPKGPEPTRY DWERNGR  DF
Sbjct: 62  KENQQLLQDLEEEFDEDEFKPYVNPITKEIGGPKGPEPTRYNDWERNGRVSDF 108

BLAST of CaUC01G000570 vs. ExPASy TrEMBL
Match: A0A0A0KQ66 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucumis sativus OX=3659 GN=Csa_5G139490 PE=3 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 5.6e-40
Identity = 93/111 (83.78%), Postives = 99/111 (89.19%), Query Frame = 0

Query: 1   MSSNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGS 60
           MSSNLSRLFSSPPTLSALPA SRLT NSLSR VSSSSNQQPSR E+GVSSEEQREAGKGS
Sbjct: 1   MSSNLSRLFSSPPTLSALPAVSRLTFNSLSRFVSSSSNQQPSRLEKGVSSEEQREAGKGS 60

Query: 61  LPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF 112
            PN+HEAA DDR G+DEGVD ++ T E+GGPKGPEPTRYGDWER GRC DF
Sbjct: 61  SPNRHEAAGDDRGGQDEGVDTEKMTREIGGPKGPEPTRYGDWERKGRCSDF 111

BLAST of CaUC01G000570 vs. ExPASy TrEMBL
Match: A0A5A7TMD3 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00530 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.4e-38
Identity = 90/111 (81.08%), Postives = 97/111 (87.39%), Query Frame = 0

Query: 1   MSSNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGS 60
           MSS+LSRLFSSPPTLSALP   RLT+NSLSR VSSSSNQQPSRSEQGVSSEEQREA KGS
Sbjct: 1   MSSHLSRLFSSPPTLSALPTVGRLTSNSLSRFVSSSSNQQPSRSEQGVSSEEQREAEKGS 60

Query: 61  LPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF 112
            PNQHEAA DDR  +D+GVD ++   E+GGPKG EPTRYGDWERNGRCYDF
Sbjct: 61  SPNQHEAAGDDRGSQDQGVDTEKMAPEIGGPKGHEPTRYGDWERNGRCYDF 111

BLAST of CaUC01G000570 vs. ExPASy TrEMBL
Match: A0A6J1K722 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111491251 PE=3 SV=1)

HSP 1 Score: 168.7 bits (426), Expect = 1.4e-38
Identity = 89/111 (80.18%), Postives = 100/111 (90.09%), Query Frame = 0

Query: 1   MSSNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGS 60
           MSSNL+RLF SPP+L+AL A +R+T+ S+SRLVSSS+NQQPSR EQGV  EEQREAGKGS
Sbjct: 1   MSSNLTRLF-SPPSLTALSAVNRVTSTSVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  LPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF 112
           LP+QHEAA DDRD EDEG D+ +ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of CaUC01G000570 vs. ExPASy TrEMBL
Match: A0A6J1H1T7 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111459345 PE=3 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 5.2e-38
Identity = 89/111 (80.18%), Postives = 99/111 (89.19%), Query Frame = 0

Query: 1   MSSNLSRLFSSPPTLSALPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSEEQREAGKGS 60
           MSSNL+RLF SPP+L+AL A +R T+ S+SRLVSSS+NQQPSR EQGV  EEQREAGKGS
Sbjct: 1   MSSNLTRLF-SPPSLTALSAVNRATSISVSRLVSSSANQQPSRLEQGVPGEEQREAGKGS 60

Query: 61  LPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF 112
           LP+QHEAA DDRD EDEG D+ +ATGEVGGPKGPEPTRYGDWERNGRCYDF
Sbjct: 61  LPDQHEAAGDDRDSEDEGADVNKATGEVGGPKGPEPTRYGDWERNGRCYDF 110

BLAST of CaUC01G000570 vs. ExPASy TrEMBL
Match: W9RP47 (Succinate dehydrogenase assembly factor 4, mitochondrial OS=Morus notabilis OX=981085 GN=L484_000138 PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.5e-16
Identity = 62/120 (51.67%), Postives = 77/120 (64.17%), Query Frame = 0

Query: 1   MSSNLSRLFSSPPTLSA---------LPAASRLTANSLSRLVSSSSNQQPSRSEQGVSSE 60
           M+SNL RLFSS   LS              +R   NS SRLVSSS+ Q+P   ++ + ++
Sbjct: 1   MASNLGRLFSSITNLSTPKVILSSTRSEPLTRCATNSASRLVSSSA-QKPQYDQENM-NK 60

Query: 61  EQREAGKGSLPNQHEAAIDDRDGEDEGVDIKEATGEVGGPKGPEPTRYGDWERNGRCYDF 112
           EQREA    L N+ E  ++D   E+EG  + E TGEVGGP+GPEPTRYGDWERNGRCYDF
Sbjct: 61  EQREAIAKDLENEKEDGVED---EEEGTSLNEQTGEVGGPRGPEPTRYGDWERNGRCYDF 115

BLAST of CaUC01G000570 vs. TAIR 10
Match: AT5G67490.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1674 (InterPro:IPR012875); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 53.1 bits (126), Expect = 1.6e-07
Identity = 32/85 (37.65%), Postives = 44/85 (51.76%), Query Frame = 0

Query: 29  LSRLVSSSSNQQPSRSEQGVSSEEQREAGKGSLPNQHEAAIDDRDGEDEGVD--IKEATG 88
           +SR +SS +   P             +  +    N      ++ +GE  G    + E TG
Sbjct: 24  VSRFLSSGTPPPPQAPSPNQDLNRDVKPDQNLQQNLQMQKEEEEEGEGGGGGEFVNEDTG 83

Query: 89  EVGGPKGPEPTRYGDWERNGRCYDF 112
           E+GGP+GPEPTRYGDWE+ GRC DF
Sbjct: 84  EIGGPRGPEPTRYGDWEQRGRCSDF 108

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KGN49871.11.2e-3983.78hypothetical protein Csa_000062 [Cucumis sativus][more]
KAA0042555.12.8e-3881.08succinate dehydrogenase assembly factor 4 [Cucumis melo var. makuwa] >TYK05959.1... [more]
XP_022995844.12.8e-3880.18succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita maxima][more]
XP_022957978.11.1e-3780.18succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita moschata][more]
XP_023532848.12.4e-3779.28succinate dehydrogenase assembly factor 4, mitochondrial [Cucurbita pepo subsp. ... [more]
Match NameE-valueIdentityDescription
Q8BTE06.0e-0743.06Succinate dehydrogenase assembly factor 4, mitochondrial OS=Mus musculus OX=1009... [more]
Q9VLU67.8e-0781.48Succinate dehydrogenase assembly factor 4, mitochondrial OS=Drosophila melanogas... [more]
Q5VUM11.0e-0643.75Succinate dehydrogenase assembly factor 4, mitochondrial OS=Homo sapiens OX=9606... [more]
Q54Y251.5e-0530.09Succinate dehydrogenase assembly factor 4, mitochondrial OS=Dictyostelium discoi... [more]
Match NameE-valueIdentityDescription
A0A0A0KQ665.6e-4083.78Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucumis sativus OX=3... [more]
A0A5A7TMD31.4e-3881.08Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucumis melo var. ma... [more]
A0A6J1K7221.4e-3880.18Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucurbita maxima OX=... [more]
A0A6J1H1T75.2e-3880.18Succinate dehydrogenase assembly factor 4, mitochondrial OS=Cucurbita moschata O... [more]
W9RP472.5e-1651.67Succinate dehydrogenase assembly factor 4, mitochondrial OS=Morus notabilis OX=9... [more]
Match NameE-valueIdentityDescription
AT5G67490.11.6e-0737.65unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF1674... [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL246-FR2) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012875Succinate dehydrogenase assembly factor 4PFAMPF07896DUF1674coord: 80..111
e-value: 3.1E-16
score: 59.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..111
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..47
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 97..111
NoneNo IPR availablePANTHERPTHR28524SUCCINATE DEHYDROGENASE ASSEMBLY FACTOR 4, MITOCHONDRIALcoord: 1..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CaUC01G000570.1CaUC01G000570.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008652 cellular amino acid biosynthetic process
biological_process GO:0009064 glutamine family amino acid metabolic process
biological_process GO:0034553 mitochondrial respiratory chain complex II assembly
cellular_component GO:0005739 mitochondrion
molecular_function GO:0043167 ion binding
molecular_function GO:0016638 oxidoreductase activity, acting on the CH-NH2 group of donors