CSPI07G06150 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI07G06150
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Phloem filament protein
Location	Chr7: 4579824 .. 4580801 (+)
RNA-Seq Expression	CSPI07G06150
Synteny	CSPI07G06150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GATACAATATATGCATGGACGTTTGAGAATTAAACAAGTTTCTAATTCGGAGGTTGACAAAACATTTTGACACACAAATATACTGAAGTGACGTAGATACGGATGGATCTTATCTCTTACAAATTTTTAGTTTGACTATCATCCATCCATCCACAATTTGATAGACTTATTTAATATATATGTGTCAAACAACCACGATTCGTGAAATAAAGCCCCAAAATTAATTAATTTTTACATAAAACTAAGGTCATCAAAATGAGTTCTTTGCCAAGTATTTGTTCCAAAAGGTGGATTAAGTTCCCTAATGTTTTGATTCCTTGTTTGCAAGCCATTGCAGAGCATGGAGTAGAAGAGTTCAAGAAAAAACATGAAGTGAGCCTGATATACAAGAACGTTGTTGAAGGTTGGTATAAGGAGTTGGATGATCATGGAAATACTATAAGGTACCGTCTTCATATTCAAGCATACGATTGTATTAGACGTCTACTCAAATTTGAAGCTGTTTTGCTCCAACAACATGCTCAGAATAATGAAAAATCTACCATCACTCTTGAGTCTTTTGAACAGATATAATTTCTTACCTCTAAAATAAAGTAAACTATAATATATATATATCAAACTTTCCTTTCCCTTTTTGTCTTGTGTTCTTCCTTCTAGCTCTCTACACACTTTGTCACTTATTTGTATGCTTTGTACTGTCTTTTGCTATCTATATATTGTTCTATCTGAATAACCATATGTAGTAATGAGTCGTACGAGTTTTTTGTATTAGGTTTGAAGCTTTTCGGGCAAGTTTTGTTTCTCTCTTTCTGTAATCGCTCTAGCAATTGTATTGCGGATCAAGGCGTTTGTTTTGAGTGAGATTATTAATAGTTTTATGAAGTGTTTCTATTGAAAAAAATAAATAAAGCTATATACCAAACTTTCCCTTTTGTGTTGTGGTCTATCTGATGATCTACAATATATATTCTTTACCGC

mRNA sequence

GATACAATATATGCATGGACGTTTGAGAATTAAACAAGTTTCTAATTCGGAGGTTGACAAAACATTTTGACACACAAATATACTGAAGTGACGTAGATACGGATGGATCTTATCTCTTACAAATTTTTAGTTTGACTATCATCCATCCATCCACAATTTGATAGACTTATTTAATATATATGTGTCAAACAACCACGATTCGTGAAATAAAGCCCCAAAATTAATTAATTTTTACATAAAACTAAGGTCATCAAAATGAGTTCTTTGCCAAGTATTTGTTCCAAAAGGTGGATTAAGTTCCCTAATGTTTTGATTCCTTGTTTGCAAGCCATTGCAGAGCATGGAGTAGAAGAGTTCAAGAAAAAACATGAAGTGAGCCTGATATACAAGAACGTTGTTGAAGGTTGGTATAAGGAGTTGGATGATCATGGAAATACTATAAGGTACCGTCTTCATATTCAAGCATACGATTGTATTAGACGTCTACTCAAATTTGAAGCTGTTTTGCTCCAACAACATGCTCAGAATAATGAAAAATCTACCATCACTCTTGAGTCTTTTGAACAGATATAATTTCTTACCTCTAAAATAAAGTAAACTATAATATATATATATCAAACTTTCCTTTCCCTTTTTGTCTTGTGTTCTTCCTTCTAGCTCTCTACACACTTTGTCACTTATTTGTATGCTTTGTACTGTCTTTTGCTATCTATATATTGTTCTATCTGAATAACCATATGTAGTAATGAGTCGTACGAGTTTTTTGTATTAGGTTTGAAGCTTTTCGGGCAAGTTTTGTTTCTCTCTTTCTGTAATCGCTCTAGCAATTGTATTGCGGATCAAGGCGTTTGTTTTGAGTGAGATTATTAATAGTTTTATGAAGTGTTTCTATTGAAAAAAATAAATAAAGCTATATACCAAACTTTCCCTTTTGTGTTGTGGTCTATCTGATGATCTACAATATATATTCTTTACCGC

Coding sequence (CDS)

ATGAGTTCTTTGCCAAGTATTTGTTCCAAAAGGTGGATTAAGTTCCCTAATGTTTTGATTCCTTGTTTGCAAGCCATTGCAGAGCATGGAGTAGAAGAGTTCAAGAAAAAACATGAAGTGAGCCTGATATACAAGAACGTTGTTGAAGGTTGGTATAAGGAGTTGGATGATCATGGAAATACTATAAGGTACCGTCTTCATATTCAAGCATACGATTGTATTAGACGTCTACTCAAATTTGAAGCTGTTTTGCTCCAACAACATGCTCAGAATAATGAAAAATCTACCATCACTCTTGAGTCTTTTGAACAGATATAA

Protein sequence

MSSLPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGNTIRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQI*

Homology

[Close]

BLAST of CSPI07G06150 vs. ExPASy TrEMBL
Match: A0A0A0K471 (Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G067340 PE=4 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.1e-53
Identity = 104/105 (99.05%), Postives = 105/105 (100.00%), Query Frame = 0


Query: 1   MSSLPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGN 60
           MSSLPSICSKRWIKFPNVLIPCLQAIAEHG+EEFKKKHEVSLIYKNVVEGWYKELDDHGN
Sbjct: 1   MSSLPSICSKRWIKFPNVLIPCLQAIAEHGIEEFKKKHEVSLIYKNVVEGWYKELDDHGN 60

Query: 61  TIRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQI 106
           TIRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQI
Sbjct: 61  TIRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQI 105

[Close]

BLAST of CSPI07G06150 vs. ExPASy TrEMBL
Match: A0A5A7SN25 (Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G001820 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 5.3e-48
Identity = 92/105 (87.62%), Postives = 103/105 (98.10%), Query Frame = 0


Query: 1   MSSLPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGN 60
           MSSLPS CSK+WIK P+VLIPCLQAIAEHGVEEFKKK++VSLIYKNVVEGWY+ELDDHGN
Sbjct: 1   MSSLPSGCSKKWIKLPSVLIPCLQAIAEHGVEEFKKKYDVSLIYKNVVEGWYQELDDHGN 60

Query: 61  TIRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQI 106
           TIRYRLH+QAYDC+RRLLKFEA+LLQQHAQNNE+STITLESF++I
Sbjct: 61  TIRYRLHVQAYDCLRRLLKFEAILLQQHAQNNEESTITLESFDRI 105

[Close]

BLAST of CSPI07G06150 vs. ExPASy TrEMBL
Match: E5GCR7 (Phloem filament protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 5.3e-48
Identity = 92/105 (87.62%), Postives = 103/105 (98.10%), Query Frame = 0


Query: 1   MSSLPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGN 60
           MSSLPS CSK+WIK P+VLIPCLQAIAEHGVEEFKKK++VSLIYKNVVEGWY+ELDDHGN
Sbjct: 1   MSSLPSGCSKKWIKLPSVLIPCLQAIAEHGVEEFKKKYDVSLIYKNVVEGWYQELDDHGN 60

Query: 61  TIRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQI 106
           TIRYRLH+QAYDC+RRLLKFEA+LLQQHAQNNE+STITLESF++I
Sbjct: 61  TIRYRLHVQAYDCLRRLLKFEAILLQQHAQNNEESTITLESFDRI 105

[Close]

BLAST of CSPI07G06150 vs. ExPASy TrEMBL
Match: A0A5D3C4U6 (Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001810 PE=4 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 6.5e-46
Identity = 89/105 (84.76%), Postives = 101/105 (96.19%), Query Frame = 0


Query: 1   MSSLPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGN 60
           MSSL S CSK+WIK P+VLIPCLQAIAEHGVEEFKKK++VSLIYKNVVEGWY+ELD HGN
Sbjct: 1   MSSLASGCSKKWIKLPSVLIPCLQAIAEHGVEEFKKKYDVSLIYKNVVEGWYQELDVHGN 60

Query: 61  TIRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQI 106
           T+RYRLH+QAYDC+RRLLKFEA+LLQQHAQNNE+STITLESF++I
Sbjct: 61  TVRYRLHVQAYDCLRRLLKFEAILLQQHAQNNEESTITLESFDRI 105

[Close]

BLAST of CSPI07G06150 vs. ExPASy TrEMBL
Match: A0A6J1EIQ8 (uncharacterized protein LOC111434517 OS=Cucurbita moschata OX=3662 GN=LOC111434517 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 8.8e-11
Identity = 36/88 (40.91%), Postives = 57/88 (64.77%), Query Frame = 0


Query: 4   LPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGNTIR 63
           L S+  ++WI+ P+V  PC+Q +AE  V+EF +K+   L + +V EGWY EL    N+++
Sbjct: 212 LSSLVVEKWIQIPSVKEPCVQVVAEFAVDEFNRKYGNILKFNSVYEGWYWELSP--NSLK 271

Query: 64  YRLHIQAYDCIRRLLKFEAVLLQQHAQN 92
           YRLHI+A D + R L +E V+ +   Q+
Sbjct: 272 YRLHIKAIDFLERCLNYEFVVFEVKYQS 297

[Close]

BLAST of CSPI07G06150 vs. NCBI nr
Match: ADN34265.1 (phloem filament protein [Cucumis melo subsp. melo] >KAA0031426.1 phloem filament protein [Cucumis melo var. makuwa])

HSP 1 Score: 199.9 bits (507), Expect = 1.1e-47
Identity = 92/105 (87.62%), Postives = 103/105 (98.10%), Query Frame = 0


Query: 1   MSSLPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGN 60
           MSSLPS CSK+WIK P+VLIPCLQAIAEHGVEEFKKK++VSLIYKNVVEGWY+ELDDHGN
Sbjct: 1   MSSLPSGCSKKWIKLPSVLIPCLQAIAEHGVEEFKKKYDVSLIYKNVVEGWYQELDDHGN 60

Query: 61  TIRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQI 106
           TIRYRLH+QAYDC+RRLLKFEA+LLQQHAQNNE+STITLESF++I
Sbjct: 61  TIRYRLHVQAYDCLRRLLKFEAILLQQHAQNNEESTITLESFDRI 105

[Close]

BLAST of CSPI07G06150 vs. NCBI nr
Match: TYK06877.1 (phloem filament protein [Cucumis melo var. makuwa])

HSP 1 Score: 193.0 bits (489), Expect = 1.3e-45
Identity = 89/105 (84.76%), Postives = 101/105 (96.19%), Query Frame = 0


Query: 1   MSSLPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGN 60
           MSSL S CSK+WIK P+VLIPCLQAIAEHGVEEFKKK++VSLIYKNVVEGWY+ELD HGN
Sbjct: 1   MSSLASGCSKKWIKLPSVLIPCLQAIAEHGVEEFKKKYDVSLIYKNVVEGWYQELDVHGN 60

Query: 61  TIRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQI 106
           T+RYRLH+QAYDC+RRLLKFEA+LLQQHAQNNE+STITLESF++I
Sbjct: 61  TVRYRLHVQAYDCLRRLLKFEAILLQQHAQNNEESTITLESFDRI 105

[Close]

BLAST of CSPI07G06150 vs. NCBI nr
Match: KAE8645824.1 (hypothetical protein Csa_017165 [Cucumis sativus])

HSP 1 Score: 135.2 bits (339), Expect = 3.3e-28
Identity = 61/62 (98.39%), Postives = 62/62 (100.00%), Query Frame = 0


Query: 1  MSSLPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGN 60
          MSSLPSICSKRWIKFPNVLIPCLQAIAEHG+EEFKKKHEVSLIYKNVVEGWYKELDDHGN
Sbjct: 1  MSSLPSICSKRWIKFPNVLIPCLQAIAEHGIEEFKKKHEVSLIYKNVVEGWYKELDDHGN 60

Query: 61 TI 63
          TI
Sbjct: 61 TI 62

[Close]

BLAST of CSPI07G06150 vs. NCBI nr
Match: XP_038887119.1 (uncharacterized protein LOC120077304 [Benincasa hispida])

HSP 1 Score: 106.3 bits (264), Expect = 1.6e-19
Identity = 53/103 (51.46%), Postives = 73/103 (70.87%), Query Frame = 0


Query: 2   SSLPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGNT 61
           S+ P  CSK+WIK  ++  PC++  A+  +EEF  KH  SL YK V+EGW KELD+  + 
Sbjct: 3   STFPCGCSKKWIKITDLETPCVEDNAKFAIEEFNIKHNGSLRYKKVIEGWCKELDE--SH 62

Query: 62  IRYRLHIQAYDCIRRLLKFEAVLLQQHAQNNEKSTITLESFEQ 105
           IRYRL +QA DC+RRLL+FEA++   +++N E + I LESFEQ
Sbjct: 63  IRYRLLVQANDCLRRLLEFEAIVFAHYSENGETTRI-LESFEQ 102

[Close]

BLAST of CSPI07G06150 vs. NCBI nr
Match: KAG6588835.1 (hypothetical protein SDJN03_17400, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 76.3 bits (186), Expect = 1.8e-10
Identity = 36/88 (40.91%), Postives = 57/88 (64.77%), Query Frame = 0


Query: 4   LPSICSKRWIKFPNVLIPCLQAIAEHGVEEFKKKHEVSLIYKNVVEGWYKELDDHGNTIR 63
           L S+  ++WI+ P+V  PC+Q +AE  V+EF +K+   L + +V EGWY EL    N+++
Sbjct: 212 LSSLVVEKWIQIPSVKEPCVQVVAEFAVDEFNRKYGNILKFNSVYEGWYWELSP--NSLK 271

Query: 64  YRLHIQAYDCIRRLLKFEAVLLQQHAQN 92
           YRLHI+A D + R L +E V+ +   Q+
Sbjct: 272 YRLHIKAIDFLERCLNYEFVVFEVKYQS 297

The following BLAST results are available for this feature:

BLAST of CSPI07G06150 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI07G06150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K471	1.1e-53	99.05	Phloem filament protein OS=Cucumis sativus OX=3659 GN=Csa_7G067340 PE=4 SV=1	[more]
A0A5A7SN25	5.3e-48	87.62	Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
E5GCR7	5.3e-48	87.62	Phloem filament protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1	[more]
A0A5D3C4U6	6.5e-46	84.76	Phloem filament protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A6J1EIQ8	8.8e-11	40.91	uncharacterized protein LOC111434517 OS=Cucurbita moschata OX=3662 GN=LOC1114345...	[more]

BLAST of CSPI07G06150 vs. NCBI nr


Match Name	E-value	Identity	Description
ADN34265.1	1.1e-47	87.62	phloem filament protein [Cucumis melo subsp. melo] >KAA0031426.1 phloem filament...	[more]
TYK06877.1	1.3e-45	84.76	phloem filament protein [Cucumis melo var. makuwa]	[more]
KAE8645824.1	3.3e-28	98.39	hypothetical protein Csa_017165 [Cucumis sativus]	[more]
XP_038887119.1	1.6e-19	51.46	uncharacterized protein LOC120077304 [Benincasa hispida]	[more]
KAG6588835.1	1.8e-10	40.91	hypothetical protein SDJN03_17400, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CSPI07G06150 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.10.450.10		coord: 13..105 e-value: 3.3E-7 score: 32.2
None	No IPR available	PANTHER	PTHR47116:SF5	PHLOEM FILAMENT PROTEIN	coord: 4..105
None	No IPR available	SUPERFAMILY	54403	Cystatin/monellin	coord: 12..94
IPR009994	Phloem filament PP1	PFAM	PF07430	PP1	coord: 21..91 e-value: 2.3E-17 score: 62.8
IPR027214	Cystatin	PANTHER	PTHR47116	PHLOEM FILAMENT PROTEIN	coord: 4..105

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI07G06150.1	CSPI07G06150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004869	cysteine-type endopeptidase inhibitor activity