CSPI06G22640 (gene) Cucumber (PI 183967) v1

Overview


Name	CSPI06G22640
Type	gene
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	RING-type domain-containing protein
Location	Chr6: 20468484 .. 20470361 (+)
RNA-Seq Expression	CSPI06G22640
Synteny	CSPI06G22640

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GCACGCTGTAGTTTTCAAGGTTTTTATTTTGACCATTTCAAATTCATATGAGCTATCATGTTCATTTGAGGACAATCTCGATATGAATACATGATTTTGATGTTCAAATAACATCCCTTGTAATGTCTAAAAGGTGTCACTTCAAGATTCTTTTTCTCCCAGAATTAGATTTGTGTTTCTGAAAGGATTTAAGCTTAGAAATGGGAGAGATGATGATAACCACCCAACACTATCATGTGTTCATGTGTCACCTACCCAAAAGATTATTCTGTATTAACAATAATTTTCTGCAGGTTTGAGATACTAGACATCATGCAGTTAGTTCAGATGGTGGGCTTTCGTTTTTTTGTAGTTCAAAGGAAGTGGAGTGAAAGATTTGAATCTCTTACATTTGGTCAAAGACACAAGGTTTGATTTGGACTAATTAGTATATGCATTAAGGCCATACATTATACACTATTTCTATTTATTACAGGTTTAGAAGGTAAATGGTGTATTCTCGCCAGACACGGTTAGCTTGAATGGCGCATAGGTCTGCGTTTTCCCGCTCAAATAGAGGCACTAATTTGAATTAGGTACTTTCCAACCCACCTGAAGTCTTTGTTAGTGATAGAGATAGAGTTATGAGTTAGAGAGAGAAATGTCAATTGTCAACAGAGCAATCAAACATGGCGGGTCCCCTTTTCCATATAGATCGAGATCGATGCCTGCACGTTTATTGCTTTACTCTCTCTTCATTGCCTGCTGCGGCGCGCTCCCCTCCACTCCAATGCTAACCACAAGGTCTTTGATCAATCACCTCATTCAATTCAACTTCACAAGCTTCCCTTAGAACCAACTTCTTCAGTTCAGATATGGAATTGGAATGAATGGCAGACATGGCATGGCCCTTCATTCTACTTCCGAGTGTCTCTCTTCTTCACCTCCACTCGACATTGGCGGACTTCTTTCAGCTCAGTTAATGACGTCCTATCTCTGAAGATCTACAGAAAACCCAAAACTGGTTAGTAGGATCATTATCTCTTTCATGAAAAACCAACTTTTCTTTTCTTGATTTTGCTCCTTTGTTTTGAGAAGACATTGTTTGAGGCATTCTTCTTTGTGGGTATCTCTGGTTTATTCTCTGTTTCATTTCACACCATAATCCCTTTTCGGGGTTCGTTGTGTCAGATTGGTACCTAGAAGAAATTTGAAGAGGAAATGGAGCGGGCTAATCGAGGGCGGAGATGGACAAATTTAAAGCAACGGCTAGGATTGAAGGGGATGGGGTGCTGTAGAGGTTCATGGAGTCCAACATCGTCAACTTTGACAATGATTGAAGGGTTTAGCTTAAGCCTTCATGGAAGCAGCCGCAGTCAAGGAGAAGATGGGGGTCGGAGCTCAGCAGCTTCCGGTATGAACTTGGCGATGGCTCTGGCGGCGGAGAGAAATTTGAGGAGTGACGAAGGAGGCCCCACGGGGGGTAGTGATGTGAAATCACTGATGAGATTGTTTGAAGAAATGGACGGTGGGGATTGGAAAACAAAAAGAAAGGAAAGTGAGAATAACGGTGATTGGATGTGCTGCGTGTGCATGGAAAGGAGTAAAGGCGCGGCTTTTATTCCCTGTGGACACACCTTCTGTAGGGTATGTTCTAGGGAACTATGGCTTAACAGAGGCACGTGTCCCATCTGCAGCCGTTCGATCATTGAGATTCTTGACATTTTTTAAAATTTTTCAAAAACTCATTTTTGTTTTCCTAAAATTATTATCTCAATGTGATTTGTTTTTGTTTTATTTTGTGGTTTTGGTATATTTTTATTTTCTTCTGATAGGTATGATGGAAGGATAAGGCTGCACATGTTCATCAAAATTACATCATCCAAATATAACATTCTA

mRNA sequence

GCACGCTGTAGTTTTCAAGGTTTTTATTTTGACCATTTCAAATTCATATGAGCTATCATGTTCATTTGAGGACAATCTCGATATGAATACATGATTTTGATGTTCAAATAACATCCCTTGTAATGTCTAAAAGGTGTCACTTCAAGATTCTTTTTCTCCCAGAATTAGATTTGTGTTTCTGAAAGGATTTAAGCTTAGAAATGGGAGAGATGATGATAACCACCCAACACTATCATGTGTTCATGTGTCACCTACCCAAAAGATTATTCTGTATTAACAATAATTTTCTGCAGGTTTGAGATACTAGACATCATGCAGTTAGTTCAGATGGTGGGCTTTCGTTTTTTTGTAGTTCAAAGGAAGTGGAGTGAAAGATTTGAATCTCTTACATTTGGTCAAAGACACAAGGTTTGATTTGGACTAATTAGTATATGCATTAAGGCCATACATTATACACTATTTCTATTTATTACAGGTTTAGAAGGTAAATGGTGTATTCTCGCCAGACACGGTTAGCTTGAATGGCGCATAGGTCTGCGTTTTCCCGCTCAAATAGAGGCACTAATTTGAATTAGGTACTTTCCAACCCACCTGAAGTCTTTGTTAGTGATAGAGATAGAGTTATGAGTTAGAGAGAGAAATGTCAATTGTCAACAGAGCAATCAAACATGGCGGGTCCCCTTTTCCATATAGATCGAGATCGATGCCTGCACGTTTATTGCTTTACTCTCTCTTCATTGCCTGCTGCGGCGCGCTCCCCTCCACTCCAATGCTAACCACAAGGTCTTTGATCAATCACCTCATTCAATTCAACTTCACAAGCTTCCCTTAGAACCAACTTCTTCAGTTCAGATATGGAATTGGAATGAATGGCAGACATGGCATGGCCCTTCATTCTACTTCCGAGTGTCTCTCTTCTTCACCTCCACTCGACATTGGCGGACTTCTTTCAGCTCAGTTAATGACGTCCTATCTCTGAAGATCTACAGAAAACCCAAAACTGATTGGTACCTAGAAGAAATTTGAAGAGGAAATGGAGCGGGCTAATCGAGGGCGGAGATGGACAAATTTAAAGCAACGGCTAGGATTGAAGGGGATGGGGTGCTGTAGAGGTTCATGGAGTCCAACATCGTCAACTTTGACAATGATTGAAGGGTTTAGCTTAAGCCTTCATGGAAGCAGCCGCAGTCAAGGAGAAGATGGGGGTCGGAGCTCAGCAGCTTCCGGTATGAACTTGGCGATGGCTCTGGCGGCGGAGAGAAATTTGAGGAGTGACGAAGGAGGCCCCACGGGGGGTAGTGATGTGAAATCACTGATGAGATTGTTTGAAGAAATGGACGGTGGGGATTGGAAAACAAAAAGAAAGGAAAGTGAGAATAACGGTGATTGGATGTGCTGCGTGTGCATGGAAAGGAGTAAAGGCGCGGCTTTTATTCCCTGTGGACACACCTTCTGTAGGGTATGTTCTAGGGAACTATGGCTTAACAGAGGCACGTGTCCCATCTGCAGCCGTTCGATCATTGAGATTCTTGACATTTTTTAAAATTTTTCAAAAACTCATTTTTGTTTTCCTAAAATTATTATCTCAATGTGATTTGTTTTTGTTTTATTTTGTGGTTTTGGTATATTTTTATTTTCTTCTGATAGGTATGATGGAAGGATAAGGCTGCACATGTTCATCAAAATTACATCATCCAAATATAACATTCTA

Coding sequence (CDS)

ATGGAGCGGGCTAATCGAGGGCGGAGATGGACAAATTTAAAGCAACGGCTAGGATTGAAGGGGATGGGGTGCTGTAGAGGTTCATGGAGTCCAACATCGTCAACTTTGACAATGATTGAAGGGTTTAGCTTAAGCCTTCATGGAAGCAGCCGCAGTCAAGGAGAAGATGGGGGTCGGAGCTCAGCAGCTTCCGGTATGAACTTGGCGATGGCTCTGGCGGCGGAGAGAAATTTGAGGAGTGACGAAGGAGGCCCCACGGGGGGTAGTGATGTGAAATCACTGATGAGATTGTTTGAAGAAATGGACGGTGGGGATTGGAAAACAAAAAGAAAGGAAAGTGAGAATAACGGTGATTGGATGTGCTGCGTGTGCATGGAAAGGAGTAAAGGCGCGGCTTTTATTCCCTGTGGACACACCTTCTGTAGGGTATGTTCTAGGGAACTATGGCTTAACAGAGGCACGTGTCCCATCTGCAGCCGTTCGATCATTGAGATTCTTGACATTTTTTAA

Protein sequence

MERANRGRRWTNLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRSSAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWMCCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF*

Homology

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BLAST of CSPI06G22640 vs. ExPASy Swiss-Prot
Match: F4ISV9 (E3 ubiquitin-protein ligase APD4 OS=Arabidopsis thaliana OX=3702 GN=APD4 PE=2 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 1.9e-04
Identity = 16/51 (31.37%), Postives = 28/51 (54.90%), Query Frame = 0


Query: 120 MCCVCMERSKGAAFIPCGH-TFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           +C +C +  +   F+PCGH   C  C  ++   +G CPIC + I+ +  I+
Sbjct: 347 LCAICFDAPRDCCFLPCGHCVSCYQCGTKIKRTKGRCPICRKKIMHVKRIY 397

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BLAST of CSPI06G22640 vs. ExPASy Swiss-Prot
Match: P43254 (E3 ubiquitin-protein ligase COP1 OS=Arabidopsis thaliana OX=3702 GN=COP1 PE=1 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 2.5e-04
Identity = 21/64 (32.81%), Postives = 33/64 (51.56%), Query Frame = 0


Query: 99  EEMDGGDWKTKRKESENNGDWMCCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPIC 158
           E  DGG   ++    + + D +C +CM+  K A    CGH+FC +C      N+  CP C
Sbjct: 30  ENDDGGSGGSEIGAPDLDKDLLCPICMQIIKDAFLTACGHSFCYMCIITHLRNKSDCPCC 89

Query: 159 SRSI 163
           S+ +
Sbjct: 90  SQHL 93

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BLAST of CSPI06G22640 vs. ExPASy Swiss-Prot
Match: O35445 (E3 ubiquitin-protein ligase RNF5 OS=Mus musculus OX=10090 GN=Rnf5 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 7.3e-04
Identity = 23/72 (31.94%), Postives = 34/72 (47.22%), Query Frame = 0


Query: 95  MRLFEEMDGGDWKTKRKESENNGDWMCCVCMERSKGAAFIPCGHTFCRVCSRELWL---- 154
           M   EE DGG     R+    +  + C +C+E ++ A    CGH +C  C  + WL    
Sbjct: 1   MAAAEEEDGGPEGPNRERGGASATFECNICLETAREAVVSVCGHLYCWPCLHQ-WLETRP 60

Query: 155 NRGTCPICSRSI 163
           +R  CP+C   I
Sbjct: 61  DRQECPVCKAGI 71

[Close]

BLAST of CSPI06G22640 vs. ExPASy Swiss-Prot
Match: Q5M807 (E3 ubiquitin-protein ligase RNF5 OS=Rattus norvegicus OX=10116 GN=Rnf5 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 7.3e-04
Identity = 23/72 (31.94%), Postives = 34/72 (47.22%), Query Frame = 0


Query: 95  MRLFEEMDGGDWKTKRKESENNGDWMCCVCMERSKGAAFIPCGHTFCRVCSRELWL---- 154
           M   EE DGG     R+    +  + C +C+E ++ A    CGH +C  C  + WL    
Sbjct: 1   MAAAEEEDGGPEGPNRERGGASATFECNICLETAREAVVSVCGHLYCWPCLHQ-WLETRP 60

Query: 155 NRGTCPICSRSI 163
           +R  CP+C   I
Sbjct: 61  DRQECPVCKAGI 71

[Close]

BLAST of CSPI06G22640 vs. ExPASy TrEMBL
Match: A0A0A0KEL6 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G421620 PE=4 SV=1)

HSP 1 Score: 345.9 bits (886), Expect = 9.6e-92
Identity = 166/169 (98.22%), Postives = 168/169 (99.41%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS 60
           MERANR RRWTNL+QRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS
Sbjct: 1   MERANRRRRWTNLRQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS 60

Query: 61  SAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM 120
           SAASGMNLAMALAAERNLR+DEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM
Sbjct: 61  SAASGMNLAMALAAERNLRNDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM 120

Query: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF
Sbjct: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 169

[Close]

BLAST of CSPI06G22640 vs. ExPASy TrEMBL
Match: A0A5A7V0I3 (Zf-C3HC4_3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1465G00070 PE=4 SV=1)

HSP 1 Score: 342.4 bits (877), Expect = 1.1e-90
Identity = 165/169 (97.63%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS 60
           MERANRGRRW NLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQ EDGGRS
Sbjct: 1   MERANRGRRWANLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQREDGGRS 60

Query: 61  SAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM 120
           SAASGMNLAMALAAERNLRSDEGGPTG SDVKSLMRLFEEMDGGDWKTKRKE ENNGDWM
Sbjct: 61  SAASGMNLAMALAAERNLRSDEGGPTGASDVKSLMRLFEEMDGGDWKTKRKEGENNGDWM 120

Query: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF
Sbjct: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 169

[Close]

BLAST of CSPI06G22640 vs. ExPASy TrEMBL
Match: A0A6J1HXI8 (RNA-binding protein MEX3B-like OS=Cucurbita maxima OX=3661 GN=LOC111468383 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.3e-83
Identity = 153/169 (90.53%), Postives = 157/169 (92.90%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS 60
           MER N GRRW NLKQRLGLKGMGCC GSWSPTSSTLTMIEG SLS HGSSRS  EDG RS
Sbjct: 1   MERQNGGRRWANLKQRLGLKGMGCCGGSWSPTSSTLTMIEGLSLSRHGSSRSHREDGARS 60

Query: 61  SAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM 120
           SA+SG+NLAMALAAERNLR+DEGGPTG  DVKSLMRLFEEMDGGDWKTKRKESEN GDWM
Sbjct: 61  SASSGVNLAMALAAERNLRTDEGGPTGAGDVKSLMRLFEEMDGGDWKTKRKESENTGDWM 120

Query: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPIC+RSIIEILDIF
Sbjct: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICNRSIIEILDIF 169

[Close]

BLAST of CSPI06G22640 vs. ExPASy TrEMBL
Match: A0A6J1GME5 (RNA-binding protein MEX3B-like OS=Cucurbita moschata OX=3662 GN=LOC111455597 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.3e-83
Identity = 153/169 (90.53%), Postives = 157/169 (92.90%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS 60
           MER N GRRW NLKQRLGLKGMGCC GSWSPTSSTLTMIEG SLS HGSSRS  EDG RS
Sbjct: 1   MERQNGGRRWANLKQRLGLKGMGCCGGSWSPTSSTLTMIEGLSLSRHGSSRSHREDGARS 60

Query: 61  SAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM 120
           SA+SG+NLAMALAAERNLR+DEGGPTG  DVKSLMRLFEEMDGGDWKTKRKESEN GDWM
Sbjct: 61  SASSGVNLAMALAAERNLRTDEGGPTGAGDVKSLMRLFEEMDGGDWKTKRKESENTGDWM 120

Query: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPIC+RSIIEILDIF
Sbjct: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICNRSIIEILDIF 169

[Close]

BLAST of CSPI06G22640 vs. ExPASy TrEMBL
Match: A0A6J1EWQ9 (uncharacterized protein LOC111437226 OS=Cucurbita moschata OX=3662 GN=LOC111437226 PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 5.9e-73
Identity = 143/170 (84.12%), Postives = 154/170 (90.59%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGL-KGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGR 60
           MER NRG+RW NLKQRLGL KG+GCC GSWS TSSTLTMI+G  LS HGSSRS  +DG R
Sbjct: 62  MERPNRGQRWANLKQRLGLMKGIGCCGGSWSSTSSTLTMIDGIGLSRHGSSRSHRDDGTR 121

Query: 61  SSAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDW 120
           SSAASGMNLAMALAAERNL ++EGGPT G +VKSL+RLFEE+DGGDWKT+RKESENNGDW
Sbjct: 122 SSAASGMNLAMALAAERNL-TEEGGPTVG-NVKSLVRLFEEIDGGDWKTRRKESENNGDW 181

Query: 121 MCCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           +CCVCMERSKGAAFIPCGHTFCRVCSREL LN GTCPICSRSIIEILDIF
Sbjct: 182 LCCVCMERSKGAAFIPCGHTFCRVCSRELRLNGGTCPICSRSIIEILDIF 229

[Close]

BLAST of CSPI06G22640 vs. NCBI nr
Match: KGN47968.1 (hypothetical protein Csa_002927 [Cucumis sativus])

HSP 1 Score: 345.9 bits (886), Expect = 2.0e-91
Identity = 166/169 (98.22%), Postives = 168/169 (99.41%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS 60
           MERANR RRWTNL+QRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS
Sbjct: 1   MERANRRRRWTNLRQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS 60

Query: 61  SAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM 120
           SAASGMNLAMALAAERNLR+DEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM
Sbjct: 61  SAASGMNLAMALAAERNLRNDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM 120

Query: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF
Sbjct: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 169

[Close]

BLAST of CSPI06G22640 vs. NCBI nr
Match: KAA0061653.1 (zf-C3HC4_3 domain-containing protein [Cucumis melo var. makuwa] >TYK05853.1 zf-C3HC4_3 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 342.4 bits (877), Expect = 2.2e-90
Identity = 165/169 (97.63%), Postives = 165/169 (97.63%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS 60
           MERANRGRRW NLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQ EDGGRS
Sbjct: 1   MERANRGRRWANLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQREDGGRS 60

Query: 61  SAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM 120
           SAASGMNLAMALAAERNLRSDEGGPTG SDVKSLMRLFEEMDGGDWKTKRKE ENNGDWM
Sbjct: 61  SAASGMNLAMALAAERNLRSDEGGPTGASDVKSLMRLFEEMDGGDWKTKRKEGENNGDWM 120

Query: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF
Sbjct: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 169

[Close]

BLAST of CSPI06G22640 vs. NCBI nr
Match: XP_022953088.1 (RNA-binding protein MEX3B-like [Cucurbita moschata] >XP_022953089.1 RNA-binding protein MEX3B-like [Cucurbita moschata] >XP_022969345.1 RNA-binding protein MEX3B-like [Cucurbita maxima] >XP_022969346.1 RNA-binding protein MEX3B-like [Cucurbita maxima] >XP_023511758.1 RNA-binding protein MEX3B-like [Cucurbita pepo subsp. pepo] >XP_023511759.1 RNA-binding protein MEX3B-like [Cucurbita pepo subsp. pepo] >KAG6572419.1 hypothetical protein SDJN03_29147, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 318.9 bits (816), Expect = 2.6e-83
Identity = 153/169 (90.53%), Postives = 157/169 (92.90%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGRS 60
           MER N GRRW NLKQRLGLKGMGCC GSWSPTSSTLTMIEG SLS HGSSRS  EDG RS
Sbjct: 1   MERQNGGRRWANLKQRLGLKGMGCCGGSWSPTSSTLTMIEGLSLSRHGSSRSHREDGARS 60

Query: 61  SAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDWM 120
           SA+SG+NLAMALAAERNLR+DEGGPTG  DVKSLMRLFEEMDGGDWKTKRKESEN GDWM
Sbjct: 61  SASSGVNLAMALAAERNLRTDEGGPTGAGDVKSLMRLFEEMDGGDWKTKRKESENTGDWM 120

Query: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPIC+RSIIEILDIF
Sbjct: 121 CCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICNRSIIEILDIF 169

[Close]

BLAST of CSPI06G22640 vs. NCBI nr
Match: XP_023531397.1 (uncharacterized protein LOC111793648 [Cucurbita pepo subsp. pepo] >XP_023531398.1 uncharacterized protein LOC111793648 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 292.0 bits (746), Expect = 3.4e-75
Identity = 146/170 (85.88%), Postives = 155/170 (91.18%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGL-KGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGR 60
           MER NRG+RW NLKQRLGL KGMGCC GSWS TSSTLTMI+G  LS HGSSRS  +DG R
Sbjct: 62  MERPNRGQRWANLKQRLGLMKGMGCCGGSWSSTSSTLTMIDGIGLSRHGSSRSHRDDGTR 121

Query: 61  SSAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDW 120
           SSAASGMNLAMALAAERNL ++EGGPT G +VKSLMRLFEE+DGGDWKT+RKESENNGDW
Sbjct: 122 SSAASGMNLAMALAAERNL-TEEGGPTVG-NVKSLMRLFEEIDGGDWKTRRKESENNGDW 181

Query: 121 MCCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           +CCVCMERSKGAAFIPCGHTFCRVCSRELWLN GTCPICSRSIIEILDIF
Sbjct: 182 LCCVCMERSKGAAFIPCGHTFCRVCSRELWLNGGTCPICSRSIIEILDIF 229

[Close]

BLAST of CSPI06G22640 vs. NCBI nr
Match: KAG6589274.1 (hypothetical protein SDJN03_17839, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 292.0 bits (746), Expect = 3.4e-75
Identity = 146/170 (85.88%), Postives = 155/170 (91.18%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGL-KGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDGGR 60
           MER NRG+RW NLKQRLGL KGMGCC GSWS TSSTLTMI+G  LS HGSSRS  +DG R
Sbjct: 62  MERPNRGQRWANLKQRLGLMKGMGCCGGSWSSTSSTLTMIDGIGLSRHGSSRSHRDDGTR 121

Query: 61  SSAASGMNLAMALAAERNLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENNGDW 120
           SSAASGMNLAMALAAERNL ++EGGPT G +VKSLMRLFEE+DGGDWKT+RKESENNGDW
Sbjct: 122 SSAASGMNLAMALAAERNL-TEEGGPTVG-NVKSLMRLFEEIDGGDWKTRRKESENNGDW 181

Query: 121 MCCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
           +CCVCMERSKGAAFIPCGHTFCRVCSRELWLN GTCPICSRSIIEILDIF
Sbjct: 182 LCCVCMERSKGAAFIPCGHTFCRVCSRELWLNGGTCPICSRSIIEILDIF 229

[Close]

BLAST of CSPI06G22640 vs. TAIR 10
Match: AT4G03965.1 (RING/U-box superfamily protein )

HSP 1 Score: 133.7 bits (335), Expect = 1.4e-31
Identity = 87/184 (47.28%), Postives = 108/184 (58.70%), Query Frame = 0


Query: 1   MERANRGRRWTNLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSR-SQGEDGGR 60
           ++R+ R +R   LK+RLG K +GCC  +W      LT   G        +R   G D   
Sbjct: 32  LDRSRRRKR--TLKERLGFKRIGCCGPTW---GLRLTSNTGEDDDEPFENRLVSGSDHVT 91

Query: 61  SSAASGMNLAMALAAERNLRSDEGGPTGG-SDVK-SLMRLFEEMDGGDWKTKRKESEN-- 120
                GMNLA ALAAERN R++E   +G  + +K SLMRL EE        +  E+E   
Sbjct: 92  GPLNQGMNLATALAAERNYRTEETEASGSLTPLKVSLMRLLEE-TAERVVVEENETERVT 151

Query: 121 ----------NGDWMCCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEI 170
                     N D +CCVCM R KGAAFIPCGHTFCRVCSRE+WLNRG+CP+C+R IIEI
Sbjct: 152 ASASSTVRGVNNDSVCCVCMGRKKGAAFIPCGHTFCRVCSREVWLNRGSCPLCNRPIIEI 209

[Close]

BLAST of CSPI06G22640 vs. TAIR 10
Match: AT4G22250.1 (RING/U-box superfamily protein )

HSP 1 Score: 126.7 bits (317), Expect = 1.8e-29
Identity = 80/177 (45.20%), Postives = 96/177 (54.24%), Query Frame = 0


Query: 8   RRWTNLKQRLGLKGMGCCRGSWSPTSSTLTMIEGFSLSLHGSSRSQGEDG----GRSSAA 67
           RR   LK+RL  K +GCC  +W+   +  T +   +         Q E G        + 
Sbjct: 38  RRKRTLKERLRFKCIGCCGPTWNLRLTNNTTLHSPTSRDDEIEELQIETGLVLESDHGSG 97

Query: 68  SGMNLAMALAAERNLRSDEGGPTGGSDVK--SLMRLFEEMDGGDWKTKRKESE------- 127
           +GMNLA AL AER  R +          +  SLMRL EE          KE+E       
Sbjct: 98  TGMNLATALEAERYNRGESTEAEADMTPRRVSLMRLLEETAEKIVDDDGKETEILTASIG 157

Query: 128 --NNGDWMCCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
                D +CCVCM R KGAAFIPCGHTFCRVCSRELWLNRG+CP+C+R IIEILDIF
Sbjct: 158 TLTGNDSVCCVCMGRKKGAAFIPCGHTFCRVCSRELWLNRGSCPLCNRPIIEILDIF 214

[Close]

BLAST of CSPI06G22640 vs. TAIR 10
Match: AT1G62370.1 (RING/U-box superfamily protein )

HSP 1 Score: 115.2 bits (287), Expect = 5.3e-26
Identity = 73/173 (42.20%), Postives = 95/173 (54.91%), Query Frame = 0


Query: 9   RWTNLKQRLGLKGMGCC--------RGSWSP--TSSTLTMIEGFSLSLHGSSRSQGEDGG 68
           +WT LKQRL    +GCC        R + +P          E  +  +  S    G +  
Sbjct: 40  KWTTLKQRLRFDWVGCCGKPLTLRLRQAETPIVVDDDDDEEESQNQVVDFSDPGTGTELD 99

Query: 69  RSSAASGMNLAMALAAER--NLRSDEGGPTGGSDVKSLMRLFEEMDGGDWKTKRKESENN 128
             +  S  NLA ALA ER  ++  +E   T       LMRL  E DG D  T    +   
Sbjct: 100 CLTRGSTRNLAEALAEERLAHVTEEETAVT----KVPLMRLLAESDGCDSTT----TWLG 159

Query: 129 GDWMCCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCPICSRSIIEILDIF 170
            D +CCVCM R KGAAFIPCGHT+CRVCSRE+W+NRGTCP+C+RSI ++LD++
Sbjct: 160 NDPLCCVCMGREKGAAFIPCGHTYCRVCSREIWMNRGTCPLCNRSIFDVLDLY 204

[Close]

BLAST of CSPI06G22640 vs. TAIR 10
Match: AT3G25030.1 (RING/U-box superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 7.2e-23
Identity = 75/193 (38.86%), Postives = 101/193 (52.33%), Query Frame = 0


Query: 1   MERANRGRR----WTNLKQRLGLKGMGCCRGSWSPTSSTLTMIE---------GFSLSLH 60
           M+R +R  R    W +L+++L LK  G    S +   S  T I          GF LS  
Sbjct: 63  MQREHRHSRDKTAWKSLREKLRLKRNGSVWISSNSIPSLDTPIPNRDNVSHQLGFLLSNS 122

Query: 61  GSSRSQGEDGGRSSAASGMNLAMALAAERNLRSDEGGPTGGSDVK----SLMRLFEEMDG 120
           G+   +      SS    + L   LA ER L + E       +V+    SLM L EE +G
Sbjct: 123 GNVTEEA-----SSVEGRIRLGAVLAEERALSAREEETPAEREVEPARMSLMELLEENEG 182

Query: 121 G-DWKTKRKESENN------GDWMCCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCP 170
              + +   E+E         +  CCVCM RSKGAAFIPCGHTFCR+CSRELW+ RG CP
Sbjct: 183 QMSFVSVDGEAEEEVAAVTAAEISCCVCMVRSKGAAFIPCGHTFCRLCSRELWVQRGNCP 242

[Close]

BLAST of CSPI06G22640 vs. TAIR 10
Match: AT3G25030.2 (RING/U-box superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 7.2e-23
Identity = 75/193 (38.86%), Postives = 101/193 (52.33%), Query Frame = 0


Query: 1   MERANRGRR----WTNLKQRLGLKGMGCCRGSWSPTSSTLTMIE---------GFSLSLH 60
           M+R +R  R    W +L+++L LK  G    S +   S  T I          GF LS  
Sbjct: 63  MQREHRHSRDKTAWKSLREKLRLKRNGSVWISSNSIPSLDTPIPNRDNVSHQLGFLLSNS 122

Query: 61  GSSRSQGEDGGRSSAASGMNLAMALAAERNLRSDEGGPTGGSDVK----SLMRLFEEMDG 120
           G+   +      SS    + L   LA ER L + E       +V+    SLM L EE +G
Sbjct: 123 GNVTEEA-----SSVEGRIRLGAVLAEERALSAREEETPAEREVEPARMSLMELLEENEG 182

Query: 121 G-DWKTKRKESENN------GDWMCCVCMERSKGAAFIPCGHTFCRVCSRELWLNRGTCP 170
              + +   E+E         +  CCVCM RSKGAAFIPCGHTFCR+CSRELW+ RG CP
Sbjct: 183 QMSFVSVDGEAEEEVAAVTAAEISCCVCMVRSKGAAFIPCGHTFCRLCSRELWVQRGNCP 242

The following BLAST results are available for this feature:

BLAST of CSPI06G22640 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4ISV9	1.9e-04	31.37	E3 ubiquitin-protein ligase APD4 OS=Arabidopsis thaliana OX=3702 GN=APD4 PE=2 SV...	[more]
P43254	2.5e-04	32.81	E3 ubiquitin-protein ligase COP1 OS=Arabidopsis thaliana OX=3702 GN=COP1 PE=1 SV...	[more]
O35445	7.3e-04	31.94	E3 ubiquitin-protein ligase RNF5 OS=Mus musculus OX=10090 GN=Rnf5 PE=1 SV=1	[more]
Q5M807	7.3e-04	31.94	E3 ubiquitin-protein ligase RNF5 OS=Rattus norvegicus OX=10116 GN=Rnf5 PE=2 SV=1	[more]

BLAST of CSPI06G22640 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KEL6	9.6e-92	98.22	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G421620 P...	[more]
A0A5A7V0I3	1.1e-90	97.63	Zf-C3HC4_3 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A6J1HXI8	1.3e-83	90.53	RNA-binding protein MEX3B-like OS=Cucurbita maxima OX=3661 GN=LOC111468383 PE=4 ...	[more]
A0A6J1GME5	1.3e-83	90.53	RNA-binding protein MEX3B-like OS=Cucurbita moschata OX=3662 GN=LOC111455597 PE=...	[more]
A0A6J1EWQ9	5.9e-73	84.12	uncharacterized protein LOC111437226 OS=Cucurbita moschata OX=3662 GN=LOC1114372...	[more]

BLAST of CSPI06G22640 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN47968.1	2.0e-91	98.22	hypothetical protein Csa_002927 [Cucumis sativus]	[more]
KAA0061653.1	2.2e-90	97.63	zf-C3HC4_3 domain-containing protein [Cucumis melo var. makuwa] >TYK05853.1 zf-C...	[more]
XP_022953088.1	2.6e-83	90.53	RNA-binding protein MEX3B-like [Cucurbita moschata] >XP_022953089.1 RNA-binding ...	[more]
XP_023531397.1	3.4e-75	85.88	uncharacterized protein LOC111793648 [Cucurbita pepo subsp. pepo] >XP_023531398....	[more]
KAG6589274.1	3.4e-75	85.88	hypothetical protein SDJN03_17839, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of CSPI06G22640 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G03965.1	1.4e-31	47.28	RING/U-box superfamily protein	[more]
AT4G22250.1	1.8e-29	45.20	RING/U-box superfamily protein	[more]
AT1G62370.1	5.3e-26	42.20	RING/U-box superfamily protein	[more]
AT3G25030.1	7.2e-23	38.86	RING/U-box superfamily protein	[more]
AT3G25030.2	7.2e-23	38.86	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 121..158 e-value: 4.9E-7 score: 39.4
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 121..159 score: 11.205767
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 99..169 e-value: 2.3E-14 score: 54.6
None	No IPR available	PFAM	PF13920	zf-C3HC4_3	coord: 120..164 e-value: 2.6E-12 score: 46.4
None	No IPR available	PANTHER	PTHR46629	OS01G0917900 PROTEIN	coord: 1..169
None	No IPR available	PANTHER	PTHR46629:SF18	LIGASE, PUTATIVE-RELATED	coord: 1..169
None	No IPR available	CDD	cd16449	RING-HC	coord: 121..158 e-value: 1.00238E-8 score: 47.0769
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 111..166

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CSPI06G22640.1	CSPI06G22640.1	mRNA