CSPI06G20780 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G20780
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionAlpha/beta-Hydrolases superfamily protein
LocationChr6: 18909196 .. 18910995 (+)
RNA-Seq ExpressionCSPI06G20780
SyntenyCSPI06G20780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGACGAACTAACCTCAAAATTTCATCCCTAATTTCCCATTACACATTTCCCCTTCGTTCAATTCCAATTCTCAGTTTTTATATAAACCCATTCATTCTTCCTAATTTCTCTCCATCATATCTTCTTCTTCTCAATTTCTTCTCCATAATTCCACCGTTTTTTTAATACTCTGTTTTCCTTCACAGTTCCGATGACCAAAGGAAAAAGCTTAGTTCTCGACGTTTCCGGTTGGCTCAGACTCTACGACGATGGCTCCGTTGATCGGACCTGGAATGGACCGCCGGAGGTCAAGTTTGTCGCCGAGTCTGTTCCGCCTCACGATGAATTCATTGACGGAGTCGCCGTTCGTGACTTGGTGATTGATCAGAACTCTGGTCTTCGTGTTCGGATCTATTTACCGGAGGTGAAATGCGGCGGCGAAGTAAAGAAACTTCCGGTTGTCGTACATTTTCACGGCGGTGGATTTTGTATTAGCGAAGCGGATTGGTTTATGTATTATCATACTTACACTAATTTTGTGAAATCCGCCGAAGCAATTTGTGTCTCTGTTTATCTCCGTCGAGCGCCGGAGCACCGTCTTCCAGCAGCGATCGAGGACGGGTTGTCCGGTCTGAAGTGGCTTCAATCGGTGGCTCTCGGTAACGAAATCGAGCCTTGGATTGTCGAAAACGCGGATTTCAACCGCGTTTTCCTAATCGGAGATAGCGCTGGTGGAAACCTTGTTCACTCTGTCGCTGCTCTAGCCGGAGAAACCGATCTCGCCCCGTTGAAATTAGCCGGTGGAATTCCAATTCATCCCGGTTTTGTTCGAGCAAAACGAAGCAAATCAGAGATGGAGAATCCACAATCGCCGTTCTTGAATCTGGATATGGTGGACAACTTTCTGAACCTCGCATTACCGGTTGGGAGTTCAAAGGATAATCCGATCACATGTCCGATGGGAAGGGCGGCGCCGCCTCTTGAAAAGCTAAATCTGCCACCGTTTTTACTGTGCGTTGCGGAGAAGGATTTGGTAATCGACACTCAAATGGAGTATTATGAAGCAATGAAGGCGGCGAATAAAGAAGTGGAGATTCTAATGAGTAAAGGAATGGGACATAGCTTTTATTTGAATAAGATTGCTTTGAAGTTAGACCCTGAAACTGCTGCTGAATCGGACCGCCTCTTCGCCGGAATTGCTCGATTCATTAAGCAACATTAGAAGCGTTTCCCGGTGAATATAATAATGCTAGTTCTTATCAACGACGTACGTGGGCGTGGGAAATTTAATTTCTTCAAAAGTTTATATTTCAAAGTTACACCTTGAGCCTTCATCTATTGATAAAATAAATCTTAAAATAACACAAATCCTACTTTAATTAATTTTGGTCTCTCCAAATTAATTTATCATACTTTTCATGCAAATAAACACGACAATATGTGGATGTAATTTTAAAATTTAACGTGAAATCCCAGAAATTTGAAACTATATGAACTAAAATTAAATTTACATAAAAAGGAAATTTAATTTCAGGATTACAATTATGGTCCCTACAAATACCATTTTTTACTTTTTCAGAAAAATAGACAAATCACAAAGCCAAACAAATAGATGAACATATCACGAAACACAACCATGCATGATGAACTGCTCGGAGTTACATATTTTGATCACGATGAAAATGAGTAGAAAAGACAATATCCTTCTCTGCTACACTATCTTTAGCCTCTTCAATATATAAATAATAAAAATGATAAAAATACAAGAAAACTCCAAAAATTTGACAGAAGGTTACGAAAGATGGACCAATTTGTAGGCCA

mRNA sequence

GGACGAACTAACCTCAAAATTTCATCCCTAATTTCCCATTACACATTTCCCCTTCGTTCAATTCCAATTCTCAGTTTTTATATAAACCCATTCATTCTTCCTAATTTCTCTCCATCATATCTTCTTCTTCTCAATTTCTTCTCCATAATTCCACCGTTTTTTTAATACTCTGTTTTCCTTCACAGTTCCGATGACCAAAGGAAAAAGCTTAGTTCTCGACGTTTCCGGTTGGCTCAGACTCTACGACGATGGCTCCGTTGATCGGACCTGGAATGGACCGCCGGAGGTCAAGTTTGTCGCCGAGTCTGTTCCGCCTCACGATGAATTCATTGACGGAGTCGCCGTTCGTGACTTGGTGATTGATCAGAACTCTGGTCTTCGTGTTCGGATCTATTTACCGGAGGTGAAATGCGGCGGCGAAGTAAAGAAACTTCCGGTTGTCGTACATTTTCACGGCGGTGGATTTTGTATTAGCGAAGCGGATTGGTTTATGTATTATCATACTTACACTAATTTTGTGAAATCCGCCGAAGCAATTTGTGTCTCTGTTTATCTCCGTCGAGCGCCGGAGCACCGTCTTCCAGCAGCGATCGAGGACGGGTTGTCCGGTCTGAAGTGGCTTCAATCGGTGGCTCTCGGTAACGAAATCGAGCCTTGGATTGTCGAAAACGCGGATTTCAACCGCGTTTTCCTAATCGGAGATAGCGCTGGTGGAAACCTTGTTCACTCTGTCGCTGCTCTAGCCGGAGAAACCGATCTCGCCCCGTTGAAATTAGCCGGTGGAATTCCAATTCATCCCGGTTTTGTTCGAGCAAAACGAAGCAAATCAGAGATGGAGAATCCACAATCGCCGTTCTTGAATCTGGATATGGTGGACAACTTTCTGAACCTCGCATTACCGGTTGGGAGTTCAAAGGATAATCCGATCACATGTCCGATGGGAAGGGCGGCGCCGCCTCTTGAAAAGCTAAATCTGCCACCGTTTTTACTGTGCGTTGCGGAGAAGGATTTGGTAATCGACACTCAAATGGAGTATTATGAAGCAATGAAGGCGGCGAATAAAGAAGTGGAGATTCTAATGAGTAAAGGAATGGGACATAGCTTTTATTTGAATAAGATTGCTTTGAAGTTAGACCCTGAAACTGCTGCTGAATCGGACCGCCTCTTCGCCGGAATTGCTCGATTCATTAAGCAACATTAGAAGCGTTTCCCGGTGAATATAATAATGCTAGTTCTTATCAACGACGTACGTGGGCGTGGGAAATTTAATTTCTTCAAAAGTTTATATTTCAAAGTTACACCTTGAGCCTTCATCTATTGATAAAATAAATCTTAAAATAACACAAATCCTACTTTAATTAATTTTGGTCTCTCCAAATTAATTTATCATACTTTTCATGCAAATAAACACGACAATATGTGGATGTAATTTTAAAATTTAACGTGAAATCCCAGAAATTTGAAACTATATGAACTAAAATTAAATTTACATAAAAAGGAAATTTAATTTCAGGATTACAATTATGGTCCCTACAAATACCATTTTTTACTTTTTCAGAAAAATAGACAAATCACAAAGCCAAACAAATAGATGAACATATCACGAAACACAACCATGCATGATGAACTGCTCGGAGTTACATATTTTGATCACGATGAAAATGAGTAGAAAAGACAATATCCTTCTCTGCTACACTATCTTTAGCCTCTTCAATATATAAATAATAAAAATGATAAAAATACAAGAAAACTCCAAAAATTTGACAGAAGGTTACGAAAGATGGACCAATTTGTAGGCCA

Coding sequence (CDS)

ATGACCAAAGGAAAAAGCTTAGTTCTCGACGTTTCCGGTTGGCTCAGACTCTACGACGATGGCTCCGTTGATCGGACCTGGAATGGACCGCCGGAGGTCAAGTTTGTCGCCGAGTCTGTTCCGCCTCACGATGAATTCATTGACGGAGTCGCCGTTCGTGACTTGGTGATTGATCAGAACTCTGGTCTTCGTGTTCGGATCTATTTACCGGAGGTGAAATGCGGCGGCGAAGTAAAGAAACTTCCGGTTGTCGTACATTTTCACGGCGGTGGATTTTGTATTAGCGAAGCGGATTGGTTTATGTATTATCATACTTACACTAATTTTGTGAAATCCGCCGAAGCAATTTGTGTCTCTGTTTATCTCCGTCGAGCGCCGGAGCACCGTCTTCCAGCAGCGATCGAGGACGGGTTGTCCGGTCTGAAGTGGCTTCAATCGGTGGCTCTCGGTAACGAAATCGAGCCTTGGATTGTCGAAAACGCGGATTTCAACCGCGTTTTCCTAATCGGAGATAGCGCTGGTGGAAACCTTGTTCACTCTGTCGCTGCTCTAGCCGGAGAAACCGATCTCGCCCCGTTGAAATTAGCCGGTGGAATTCCAATTCATCCCGGTTTTGTTCGAGCAAAACGAAGCAAATCAGAGATGGAGAATCCACAATCGCCGTTCTTGAATCTGGATATGGTGGACAACTTTCTGAACCTCGCATTACCGGTTGGGAGTTCAAAGGATAATCCGATCACATGTCCGATGGGAAGGGCGGCGCCGCCTCTTGAAAAGCTAAATCTGCCACCGTTTTTACTGTGCGTTGCGGAGAAGGATTTGGTAATCGACACTCAAATGGAGTATTATGAAGCAATGAAGGCGGCGAATAAAGAAGTGGAGATTCTAATGAGTAAAGGAATGGGACATAGCTTTTATTTGAATAAGATTGCTTTGAAGTTAGACCCTGAAACTGCTGCTGAATCGGACCGCCTCTTCGCCGGAATTGCTCGATTCATTAAGCAACATTAG

Protein sequence

MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSVYLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHSVAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGSSKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKGMGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH*
Homology
BLAST of CSPI06G20780 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 3.2e-43
Identity = 102/304 (33.55%), Postives = 161/304 (52.96%), Query Frame = 0

Query: 10  DVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVRIYL 69
           D  G L+L  +G+V R+      +  + + +P  +     V  +D +  + + L +R+Y 
Sbjct: 13  DCMGLLQLLSNGTVLRS----ESIDLITQQIPFKNN--QTVLFKDSIYHKPNNLHLRLYK 72

Query: 70  PEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSVYLRRAPEHR 129
           P          LPVVV FHGGGFC     W  +++       S  A+ VS   R APEHR
Sbjct: 73  P--ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHR 132

Query: 130 LPAAIEDGLSGLKWLQSVALGNEIEPWIVE--NADFNRVFLIGDSAGGNLVHSVAAL--A 189
           LPAA ED  + L WL   A+ + +  W  +  + DF+RVF++GDS+GGN+ H +A    +
Sbjct: 133 LPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGS 192

Query: 190 GETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGSSKDNP 249
           G  +L P+++ G + + P F   +R+ SE   P    L+LD++D F  L+LP G+++D+ 
Sbjct: 193 GSIELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHH 252

Query: 250 ITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEY-YEAMKAANKEVEILMSKGMGHS 309
           +  P G  +P LE ++L P L+ V   +L+ D   EY Y+  K   K V+ +  +   H 
Sbjct: 253 MANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHG 307

BLAST of CSPI06G20780 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 2.7e-42
Identity = 107/304 (35.20%), Postives = 166/304 (54.61%), Query Frame = 0

Query: 7   LVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVR 66
           +V +V G +++Y DG V+R+   P         V P      GV   D+VID+ + +  R
Sbjct: 23  VVDEVEGLIKVYKDGHVERSQLLP--------CVDPSLPLELGVTCSDVVIDKLTNVWAR 82

Query: 67  IYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSVYLRRAP 126
           +Y+P       V KLP++V+FHGGGFC+  A W  Y+         +  + +SV  R AP
Sbjct: 83  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 142

Query: 127 EHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHSVAA-LA 186
           E+ LPAA EDG++ + WL      N    W  +  DF R+FL GDSAGGN+   VAA LA
Sbjct: 143 ENPLPAAYEDGVNAILWLNKARNDN---LW-AKQCDFGRIFLAGDSAGGNIAQQVAARLA 202

Query: 187 GETDLAPLKLAGGIPIHPGFVRAKRSKSE--MENPQSPFLNLDMVDNFLNLALPVGSSKD 246
              DLA LK+ G I I P +   +R++SE  + N ++  L L   D +  ++LP G++++
Sbjct: 203 SPEDLA-LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANRE 262

Query: 247 NPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKGMGH 306
           +P   P+      ++   +   L+CVAE DL++D+ ME  +  +   K V   + KG+GH
Sbjct: 263 HPYCKPVKMI---IKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDVIKRV---LHKGVGH 307

Query: 307 SFYL 308
           +F++
Sbjct: 323 AFHI 307

BLAST of CSPI06G20780 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.5e-42
Identity = 123/345 (35.65%), Postives = 177/345 (51.30%), Query Frame = 0

Query: 2   TKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAES-VPPHDEFID-GVAVRDLVIDQ 61
           T  K +  ++   LR+Y DG+V+R         F+  S VPP  E  + GV+ +D+VI +
Sbjct: 8   TTSKEIDRELPPLLRVYKDGTVER---------FLGSSFVPPSPEDPETGVSTKDIVISE 67

Query: 62  NSGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKS-AEAICV 121
           N  +  R+YLP  K     +KLP++V++HGG FC+  A  F+ +  Y N V S A  + V
Sbjct: 68  NPTISARVYLP--KLNNTTEKLPILVYYHGGAFCLESAFSFL-HQRYLNIVASKANVLVV 127

Query: 122 SVYLRRAPEHRLPAAIEDGLSGLKWLQSVALGN----EIEPWIVENADFNRVFLIGDSAG 181
           S+  R APEH LPAA EDG   LKW+ S +  N      +PW++++ DFNR ++ GD++G
Sbjct: 128 SIEYRLAPEHPLPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSG 187

Query: 182 GNLVHSVAALAGETDL-APLKLAGGIPIHPGFVRAKRSKSEM---ENPQSPFLNLDMVDN 241
            N+ H+ A   G   L   L++AG +   P F  +K   SE        SP      V N
Sbjct: 188 ANIAHNAALRVGAEALPGGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPM----QVWN 247

Query: 242 FLNLALPVGSSKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAA- 301
           F+    P G   DNP+  P+   AP L  L  P  L+ VA KD + D  + YYEA+K + 
Sbjct: 248 FVYPDAPGGI--DNPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESG 307

Query: 302 -NKEVEILMSKGMGHSFYLNKIALKLDPETAAESDRLFAGIARFI 334
              +VE+   +G  H F +        PET    D L   IA F+
Sbjct: 308 WKGDVELAQYEGEEHCFQI------YHPETENSKD-LIGRIASFL 327

BLAST of CSPI06G20780 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 5.1e-41
Identity = 101/307 (32.90%), Postives = 166/307 (54.07%), Query Frame = 0

Query: 7   LVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVR 66
           +V ++ G +++++DG V+R    PP V  V+ ++ P  +        D+ +  ++    R
Sbjct: 28  VVEEIEGLIKVFNDGCVER----PPIVPIVSPTIHPSSK----ATAFDIKLSNDTW--TR 87

Query: 67  IYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSVYLRRAP 126
           +Y+P+         LP++V+FHGGGFC+  A W  Y+   T+    A  + VSV  R AP
Sbjct: 88  VYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAP 147

Query: 127 EHRLPAAIEDGLSGLKWL--QSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHSVAA- 186
           EHRLPAA +DG++ + WL  Q ++ G     W+    + + VFL GDSAG N+ + VA  
Sbjct: 148 EHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWL-SKCNLSNVFLAGDSAGANIAYQVAVR 207

Query: 187 -LAGETDLAPLKLAGGIPIHPGFVRAKRSKSEME--NPQSPFLNLDMVDNFLNLALPVGS 246
            +A       L L G I IHP F    R+ SE +  + +S  L L   D +  LALP G+
Sbjct: 208 IMASGKYANTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGA 267

Query: 247 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 306
           S+D+P   P+  +A       LP  ++ +AE D++ +  +E  + M++  K VE ++  G
Sbjct: 268 SRDHPWCNPLMSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGG 319

Query: 307 MGHSFYL 308
           +GH+F++
Sbjct: 328 VGHAFHI 319

BLAST of CSPI06G20780 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.9e-40
Identity = 102/304 (33.55%), Postives = 162/304 (53.29%), Query Frame = 0

Query: 7   LVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVR 66
           +VLD+  +L +Y  G ++R         F+  +V P       VA +D+VID  +G+ VR
Sbjct: 83  IVLDLKPFLIIYKSGRIER---------FLGTTVIP---ACPEVATKDVVIDPATGVSVR 142

Query: 67  IYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSVYLRRAP 126
           +YLP V      KKLPV+V+FHGGGF I       Y++  T     A  + VS+  R AP
Sbjct: 143 LYLPNV-VDLPSKKLPVLVYFHGGGFVIENTGSPNYHNYLTLLAAKAGVLIVSINYRLAP 202

Query: 127 EHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHSVAALAG 186
           E+ LPA+ +D ++G  W+ S + G  +EPW+ ++ DF+++ L GDSAGGN+ H VA  A 
Sbjct: 203 EYPLPASYDDCMAGFNWVVSHSAGPALEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRAD 262

Query: 187 ETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGSSKDNPI 246
                   + G   +HP F+ ++   +E+ +P     N++  D    LA P     D+P+
Sbjct: 263 AG-----VIEGVAIVHPYFLGSEPVGNEINDP----ANIEFHDKLWRLAAPDTEGLDDPL 322

Query: 247 TCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAA--NKEVEILMSKGMGHS 306
             P+   AP L  L     ++ VA  D +++    YYEA+  +    E E++  +G+GH 
Sbjct: 323 INPVAPGAPSLAGLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWRGEAELVQHEGVGHV 364

Query: 307 FYLN 309
           F+L+
Sbjct: 383 FHLS 364

BLAST of CSPI06G20780 vs. ExPASy TrEMBL
Match: A0A0A0KDX8 (Catalytic OS=Cucumis sativus OX=3659 GN=Csa_6G401340 PE=4 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 9.2e-195
Identity = 334/336 (99.40%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60
           MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN
Sbjct: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60

Query: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120
           SGLRVRIYLPEVKCGGEVKKLPVV+HFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCGGEVKKLPVVLHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHS 180
           YLRRAPEHRLPAAIEDGLSGLKWLQSVALG+EIEPWIVENADFNRVFLIGDSAGGNLVHS
Sbjct: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGDEIEPWIVENADFNRVFLIGDSAGGNLVHS 180

Query: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240
           VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS
Sbjct: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240

Query: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300
           SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG
Sbjct: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 337
           MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 336

BLAST of CSPI06G20780 vs. ExPASy TrEMBL
Match: A0A5A7V9G7 (Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001000 PE=4 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 1.7e-185
Identity = 318/336 (94.64%), Postives = 323/336 (96.13%), Query Frame = 0

Query: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60
           MTKGKSLVLDVSGWLRLYDDGSVDRTW GPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN
Sbjct: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWTGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60

Query: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120
           SGLRVRIYLPEVKCG EVKKLPVVVHFHGGGFCISEADWFMYYHTYTN  KSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCGAEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNLAKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHS 180
           YLRRAPEHRLPA IEDG S LKWLQSVALGNEIEPWIVE ADFNRVFLIGDS+GGNLVHS
Sbjct: 121 YLRRAPEHRLPAQIEDGFSALKWLQSVALGNEIEPWIVEKADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240
           VAA AGETDLAPLKLAGGIPIHPGFVRAKRSKSE+ENPQSPFL LDMVD FLNLALPVGS
Sbjct: 181 VAARAGETDLAPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLNLALPVGS 240

Query: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300
           SKD+PITCPMG AAPPLE LNLPPFLLC+AEKDLVIDT+MEYYEAMKAANKEVEILMSKG
Sbjct: 241 SKDHPITCPMGSAAPPLENLNLPPFLLCIAEKDLVIDTEMEYYEAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 337
           MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 336

BLAST of CSPI06G20780 vs. ExPASy TrEMBL
Match: A0A1S3BCL7 (probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103488436 PE=4 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 3.0e-185
Identity = 318/336 (94.64%), Postives = 323/336 (96.13%), Query Frame = 0

Query: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60
           MTKGKSLVLDVSGWLRLYDDGSVDRTW GPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN
Sbjct: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWTGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60

Query: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120
           SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTN  KSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNLAKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHS 180
           YLRRAPEHRLPA IEDG S LKWLQSVALGNEIEPWIVE ADFNRVFLIGDS+GGNLVHS
Sbjct: 121 YLRRAPEHRLPAQIEDGFSALKWLQSVALGNEIEPWIVEKADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240
           VAA AGETDLAPLKLAGGIPIHPGFVRAKRSKSE+ENPQSPFL LDMVD FLNLALPVGS
Sbjct: 181 VAARAGETDLAPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLNLALPVGS 240

Query: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300
           SKD+PITCPMG AAPPLE LNLPPFLLC+AEKDLVIDT+MEYYEAMKAANKEVEILMSK 
Sbjct: 241 SKDHPITCPMGSAAPPLENLNLPPFLLCIAEKDLVIDTEMEYYEAMKAANKEVEILMSKE 300

Query: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 337
           MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 336

BLAST of CSPI06G20780 vs. ExPASy TrEMBL
Match: A0A6J1K8Y5 (probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111492182 PE=4 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.3e-172
Identity = 292/336 (86.90%), Postives = 317/336 (94.35%), Query Frame = 0

Query: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60
           MT GKS++LDVSGWLRL+DDGSVDRTW GPPEVKFVAESVPPHD+FIDGVAVRDLVID+ 
Sbjct: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60

Query: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120
           SGLRVRIYLPEVKC G  +KLPVVVHFHGGGFCISEADWFMYYHTYT+ VKSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCSGG-EKLPVVVHFHGGGFCISEADWFMYYHTYTDVVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHS 180
           YLRRAPEHRLPAA++DG S L+WLQSVALG+EIEPWI+ENADFNRVFLIGDS+GGNLVHS
Sbjct: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWIIENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240
           VAALAGE DL+P+KLAGGIPIHPGFVR++RSKSE+ENPQSPFL LDMVD FL+LALP GS
Sbjct: 181 VAALAGEADLSPVKLAGGIPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240

Query: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300
           +KD+PITCPMG  A PLE LNLPPFLLC+AEKDL+IDT+MEYYEAMKAANKEVEILMSKG
Sbjct: 241 TKDHPITCPMGANAQPLESLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 337
           MGHSFYLNKIALK+DPETAA SD LFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 335

BLAST of CSPI06G20780 vs. ExPASy TrEMBL
Match: A0A6J1HAY3 (probable carboxylesterase 15 OS=Cucurbita moschata OX=3662 GN=LOC111462421 PE=4 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 3.7e-172
Identity = 293/336 (87.20%), Postives = 315/336 (93.75%), Query Frame = 0

Query: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60
           MT GKS+VLDVSGWLRL+DDGSVDRTW GPPEVKFVAESVPPHD+FIDGVAVRDLVID+ 
Sbjct: 1   MTNGKSIVLDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60

Query: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120
           SGL VRIYLPEVKC G  +KLPVVVHFHGGGFCISEADWFMYYHTYT+ VKSAEAICVSV
Sbjct: 61  SGLCVRIYLPEVKCSGG-EKLPVVVHFHGGGFCISEADWFMYYHTYTDVVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHS 180
           YLRRAPEHRLPAA++DG S L+WLQSVALG+EIEPWI ENADFNRVFLIGDS+GGNLVHS
Sbjct: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240
           VAALAGE DLAP+KLAGGIPIHPGFVR++RSKSE+ENPQSPFL LDMVD FL+LALP GS
Sbjct: 181 VAALAGEADLAPVKLAGGIPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240

Query: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300
           +KD+PITCPMG  APPLE LNLPPFLLC+AEKDL+IDT+MEYY AMKAANKEVEILMSKG
Sbjct: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYRAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 337
           MGHSFYLNKIALK+DPETAA SD LFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 335

BLAST of CSPI06G20780 vs. NCBI nr
Match: XP_004146771.1 (probable carboxylesterase 15 [Cucumis sativus] >KGN47763.1 hypothetical protein Csa_003699 [Cucumis sativus])

HSP 1 Score: 689.1 bits (1777), Expect = 1.9e-194
Identity = 334/336 (99.40%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60
           MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN
Sbjct: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60

Query: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120
           SGLRVRIYLPEVKCGGEVKKLPVV+HFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCGGEVKKLPVVLHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHS 180
           YLRRAPEHRLPAAIEDGLSGLKWLQSVALG+EIEPWIVENADFNRVFLIGDSAGGNLVHS
Sbjct: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGDEIEPWIVENADFNRVFLIGDSAGGNLVHS 180

Query: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240
           VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS
Sbjct: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240

Query: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300
           SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG
Sbjct: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 337
           MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 336

BLAST of CSPI06G20780 vs. NCBI nr
Match: KAA0064793.1 (putative carboxylesterase 15 [Cucumis melo var. makuwa])

HSP 1 Score: 658.3 bits (1697), Expect = 3.6e-185
Identity = 318/336 (94.64%), Postives = 323/336 (96.13%), Query Frame = 0

Query: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60
           MTKGKSLVLDVSGWLRLYDDGSVDRTW GPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN
Sbjct: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWTGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60

Query: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120
           SGLRVRIYLPEVKCG EVKKLPVVVHFHGGGFCISEADWFMYYHTYTN  KSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCGAEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNLAKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHS 180
           YLRRAPEHRLPA IEDG S LKWLQSVALGNEIEPWIVE ADFNRVFLIGDS+GGNLVHS
Sbjct: 121 YLRRAPEHRLPAQIEDGFSALKWLQSVALGNEIEPWIVEKADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240
           VAA AGETDLAPLKLAGGIPIHPGFVRAKRSKSE+ENPQSPFL LDMVD FLNLALPVGS
Sbjct: 181 VAARAGETDLAPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLNLALPVGS 240

Query: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300
           SKD+PITCPMG AAPPLE LNLPPFLLC+AEKDLVIDT+MEYYEAMKAANKEVEILMSKG
Sbjct: 241 SKDHPITCPMGSAAPPLENLNLPPFLLCIAEKDLVIDTEMEYYEAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 337
           MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 336

BLAST of CSPI06G20780 vs. NCBI nr
Match: XP_008445398.1 (PREDICTED: probable carboxylesterase 15 [Cucumis melo])

HSP 1 Score: 657.5 bits (1695), Expect = 6.1e-185
Identity = 318/336 (94.64%), Postives = 323/336 (96.13%), Query Frame = 0

Query: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60
           MTKGKSLVLDVSGWLRLYDDGSVDRTW GPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN
Sbjct: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWTGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60

Query: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120
           SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTN  KSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNLAKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHS 180
           YLRRAPEHRLPA IEDG S LKWLQSVALGNEIEPWIVE ADFNRVFLIGDS+GGNLVHS
Sbjct: 121 YLRRAPEHRLPAQIEDGFSALKWLQSVALGNEIEPWIVEKADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240
           VAA AGETDLAPLKLAGGIPIHPGFVRAKRSKSE+ENPQSPFL LDMVD FLNLALPVGS
Sbjct: 181 VAARAGETDLAPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLNLALPVGS 240

Query: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300
           SKD+PITCPMG AAPPLE LNLPPFLLC+AEKDLVIDT+MEYYEAMKAANKEVEILMSK 
Sbjct: 241 SKDHPITCPMGSAAPPLENLNLPPFLLCIAEKDLVIDTEMEYYEAMKAANKEVEILMSKE 300

Query: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 337
           MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 336

BLAST of CSPI06G20780 vs. NCBI nr
Match: XP_038886113.1 (probable carboxylesterase 15 [Benincasa hispida])

HSP 1 Score: 635.2 bits (1637), Expect = 3.2e-178
Identity = 303/336 (90.18%), Postives = 321/336 (95.54%), Query Frame = 0

Query: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60
           MTK KSL+LDVSGWLRL+DDGSVDRTW GPPEVKFVAESVPPHD+FIDGVAVRDLVIDQN
Sbjct: 1   MTKEKSLILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQN 60

Query: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120
           SGLRVRIYLPEVKC G  KKLPV+VHFHGGGFCISEADWFMYYHTYTN V+SAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKC-GRGKKLPVIVHFHGGGFCISEADWFMYYHTYTNLVRSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHS 180
           YLRRAPEHRLPAAI+DG S LKWLQSVALGNEIEPWIVENADFNRVFLIGDS+GGNLVHS
Sbjct: 121 YLRRAPEHRLPAAIDDGFSALKWLQSVALGNEIEPWIVENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240
           VAALAGETDL PLKLAGGIPIHPGFVRAKRSKSEMENPQSPFL LDMVD FL+LALPVGS
Sbjct: 181 VAALAGETDLTPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLTLDMVDKFLSLALPVGS 240

Query: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300
           SKD+PITCPMG  APPLE+LNLPPFLLC+A+KDL+IDT+MEYY+AMK ANKEVE+LMSKG
Sbjct: 241 SKDHPITCPMGIDAPPLERLNLPPFLLCIADKDLIIDTEMEYYKAMKVANKEVELLMSKG 300

Query: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 337
           MGHSFYLNKIA+K+DPETAAESD LFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIAMKMDPETAAESDHLFAGIARFIKQH 335

BLAST of CSPI06G20780 vs. NCBI nr
Match: XP_023547302.1 (probable carboxylesterase 15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618.6 bits (1594), Expect = 3.1e-173
Identity = 293/336 (87.20%), Postives = 316/336 (94.05%), Query Frame = 0

Query: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60
           MT GKS++LDVSGWLRL+DDGSVDRTW GPPEVKFVAESVPPHD+FIDGVAVRDLVID+ 
Sbjct: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60

Query: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120
           SGL VRIYLPEVKC G  +KLPVVVHFHGGGFCISEADWFMYYHTYT+ VKSAEAICVSV
Sbjct: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHS 180
           YLRRAPEHRLPAA++DG S L+WLQSVALG+EIEPWI ENADFNRVFLIGDS+GGNLVHS
Sbjct: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240
           VAALAGE DLAP+KLAGGIPIHPGFVRA+RSKSE+ENPQSPFL LDMVD FL+LALP GS
Sbjct: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240

Query: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300
           +KD+PITCPMG  APPLE LNLPPFLLC+AEKDL+IDT+MEYY+AMKAANKEVEILMSKG
Sbjct: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 337
           MGHSFYLNKIALK+DPETAA SD LFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336

BLAST of CSPI06G20780 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.3e-44
Identity = 102/304 (33.55%), Postives = 161/304 (52.96%), Query Frame = 0

Query: 10  DVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVRIYL 69
           D  G L+L  +G+V R+      +  + + +P  +     V  +D +  + + L +R+Y 
Sbjct: 13  DCMGLLQLLSNGTVLRS----ESIDLITQQIPFKNN--QTVLFKDSIYHKPNNLHLRLYK 72

Query: 70  PEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSVYLRRAPEHR 129
           P          LPVVV FHGGGFC     W  +++       S  A+ VS   R APEHR
Sbjct: 73  P--ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHR 132

Query: 130 LPAAIEDGLSGLKWLQSVALGNEIEPWIVE--NADFNRVFLIGDSAGGNLVHSVAAL--A 189
           LPAA ED  + L WL   A+ + +  W  +  + DF+RVF++GDS+GGN+ H +A    +
Sbjct: 133 LPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGS 192

Query: 190 GETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGSSKDNP 249
           G  +L P+++ G + + P F   +R+ SE   P    L+LD++D F  L+LP G+++D+ 
Sbjct: 193 GSIELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHH 252

Query: 250 ITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEY-YEAMKAANKEVEILMSKGMGHS 309
           +  P G  +P LE ++L P L+ V   +L+ D   EY Y+  K   K V+ +  +   H 
Sbjct: 253 MANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHG 307

BLAST of CSPI06G20780 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 177.2 bits (448), Expect = 2.3e-44
Identity = 102/304 (33.55%), Postives = 161/304 (52.96%), Query Frame = 0

Query: 10  DVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVRIYL 69
           D  G L+L  +G+V R+      +  + + +P  +     V  +D +  + + L +R+Y 
Sbjct: 13  DCMGLLQLLSNGTVLRS----ESIDLITQQIPFKNN--QTVLFKDSIYHKPNNLHLRLYK 72

Query: 70  PEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSVYLRRAPEHR 129
           P          LPVVV FHGGGFC     W  +++       S  A+ VS   R APEHR
Sbjct: 73  P--ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHR 132

Query: 130 LPAAIEDGLSGLKWLQSVALGNEIEPWIVE--NADFNRVFLIGDSAGGNLVHSVAAL--A 189
           LPAA ED  + L WL   A+ + +  W  +  + DF+RVF++GDS+GGN+ H +A    +
Sbjct: 133 LPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGS 192

Query: 190 GETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGSSKDNP 249
           G  +L P+++ G + + P F   +R+ SE   P    L+LD++D F  L+LP G+++D+ 
Sbjct: 193 GSIELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHH 252

Query: 250 ITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEY-YEAMKAANKEVEILMSKGMGHS 309
           +  P G  +P LE ++L P L+ V   +L+ D   EY Y+  K   K V+ +  +   H 
Sbjct: 253 MANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHG 307

BLAST of CSPI06G20780 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 174.1 bits (440), Expect = 1.9e-43
Identity = 107/304 (35.20%), Postives = 166/304 (54.61%), Query Frame = 0

Query: 7   LVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVR 66
           +V +V G +++Y DG V+R+   P         V P      GV   D+VID+ + +  R
Sbjct: 23  VVDEVEGLIKVYKDGHVERSQLLP--------CVDPSLPLELGVTCSDVVIDKLTNVWAR 82

Query: 67  IYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSVYLRRAP 126
           +Y+P       V KLP++V+FHGGGFC+  A W  Y+         +  + +SV  R AP
Sbjct: 83  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 142

Query: 127 EHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHSVAA-LA 186
           E+ LPAA EDG++ + WL      N    W  +  DF R+FL GDSAGGN+   VAA LA
Sbjct: 143 ENPLPAAYEDGVNAILWLNKARNDN---LW-AKQCDFGRIFLAGDSAGGNIAQQVAARLA 202

Query: 187 GETDLAPLKLAGGIPIHPGFVRAKRSKSE--MENPQSPFLNLDMVDNFLNLALPVGSSKD 246
              DLA LK+ G I I P +   +R++SE  + N ++  L L   D +  ++LP G++++
Sbjct: 203 SPEDLA-LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANRE 262

Query: 247 NPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKGMGH 306
           +P   P+      ++   +   L+CVAE DL++D+ ME  +  +   K V   + KG+GH
Sbjct: 263 HPYCKPVKMI---IKSSTVTRTLVCVAEMDLLMDSNMEMCDGNEDVIKRV---LHKGVGH 307

Query: 307 SFYL 308
           +F++
Sbjct: 323 AFHI 307

BLAST of CSPI06G20780 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 169.9 bits (429), Expect = 3.6e-42
Identity = 101/307 (32.90%), Postives = 166/307 (54.07%), Query Frame = 0

Query: 7   LVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVR 66
           +V ++ G +++++DG V+R    PP V  V+ ++ P  +        D+ +  ++    R
Sbjct: 28  VVEEIEGLIKVFNDGCVER----PPIVPIVSPTIHPSSK----ATAFDIKLSNDTW--TR 87

Query: 67  IYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSVYLRRAP 126
           +Y+P+         LP++V+FHGGGFC+  A W  Y+   T+    A  + VSV  R AP
Sbjct: 88  VYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAP 147

Query: 127 EHRLPAAIEDGLSGLKWL--QSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHSVAA- 186
           EHRLPAA +DG++ + WL  Q ++ G     W+    + + VFL GDSAG N+ + VA  
Sbjct: 148 EHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWL-SKCNLSNVFLAGDSAGANIAYQVAVR 207

Query: 187 -LAGETDLAPLKLAGGIPIHPGFVRAKRSKSEME--NPQSPFLNLDMVDNFLNLALPVGS 246
            +A       L L G I IHP F    R+ SE +  + +S  L L   D +  LALP G+
Sbjct: 208 IMASGKYANTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGA 267

Query: 247 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 306
           S+D+P   P+  +A       LP  ++ +AE D++ +  +E  + M++  K VE ++  G
Sbjct: 268 SRDHPWCNPLMSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGG 319

Query: 307 MGHSFYL 308
           +GH+F++
Sbjct: 328 VGHAFHI 319

BLAST of CSPI06G20780 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 161.0 bits (406), Expect = 1.7e-39
Identity = 111/334 (33.23%), Postives = 173/334 (51.80%), Query Frame = 0

Query: 7   LVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQNSGLRVR 66
           + +D S  L++Y  G ++R             +VPP  E  +GV  +D+V   ++ L VR
Sbjct: 5   IAVDCSPLLKIYKSGRIERLMG--------EATVPPSSEPQNGVVSKDVVYSADNNLSVR 64

Query: 67  IYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTY-TNFVKSAEAICVSVYLRRA 126
           IYLPE        KLP++V+FHGGGF I E  +   YHT+ T  V ++  + VSV  RRA
Sbjct: 65  IYLPEKAAAETDSKLPLLVYFHGGGFII-ETAFSPTYHTFLTTSVSASNCVAVSVDYRRA 124

Query: 127 PEHRLPAAIEDGLSGLKWLQSVALGNEIEPWIVENADFNRVFLIGDSAGGNLVHSVAALA 186
           PEH +    +D  + LKW+ +   G+  E W+ ++ADF+RVFL GDSAG N+VH +A  A
Sbjct: 125 PEHPISVPFDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRA 184

Query: 187 GETDLAP----LKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDM-VDNFLNLALPVG- 246
            +  L+P      ++G I +HP F     SK+ ++   +    L M ++ F  +A P   
Sbjct: 185 AKEKLSPGLNDTGISGIILLHPYF----WSKTPIDEKDTKDETLRMKIEAFWMMASPNSK 244

Query: 247 SSKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAA--NKEVEILM 306
              D+P+   +   +  L  L     L+ VAEKD ++     Y   ++ +    EVE++ 
Sbjct: 245 DGTDDPLLNVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVE 304

Query: 307 SKGMGHSFYLNKIALKLDPETAAESDRLFAGIAR 332
           S+G  H F+L    LK + + A E    F+G  +
Sbjct: 305 SEGEDHVFHL----LKPECDNAIEVMHKFSGFIK 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FG133.2e-4333.55Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q9SX252.7e-4235.20Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q5NUF43.5e-4235.652-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ... [more]
Q9LFR75.1e-4132.90Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
I4DST81.9e-4033.55Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0KDX89.2e-19599.40Catalytic OS=Cucumis sativus OX=3659 GN=Csa_6G401340 PE=4 SV=1[more]
A0A5A7V9G71.7e-18594.64Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3BCL73.0e-18594.64probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103488436 PE=4 SV=1[more]
A0A6J1K8Y51.3e-17286.90probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111492182 PE=4 SV... [more]
A0A6J1HAY33.7e-17287.20probable carboxylesterase 15 OS=Cucurbita moschata OX=3662 GN=LOC111462421 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_004146771.11.9e-19499.40probable carboxylesterase 15 [Cucumis sativus] >KGN47763.1 hypothetical protein ... [more]
KAA0064793.13.6e-18594.64putative carboxylesterase 15 [Cucumis melo var. makuwa][more]
XP_008445398.16.1e-18594.64PREDICTED: probable carboxylesterase 15 [Cucumis melo][more]
XP_038886113.13.2e-17890.18probable carboxylesterase 15 [Benincasa hispida][more]
XP_023547302.13.1e-17387.20probable carboxylesterase 15 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT5G06570.12.3e-4433.55alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.22.3e-4433.55alpha/beta-Hydrolases superfamily protein [more]
AT1G68620.11.9e-4335.20alpha/beta-Hydrolases superfamily protein [more]
AT5G16080.13.6e-4232.90carboxyesterase 17 [more]
AT3G48690.11.7e-3933.23alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 84..306
e-value: 2.5E-48
score: 164.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 7..335
e-value: 1.9E-73
score: 249.7
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 7..336
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 4..333
NoneNo IPR availablePANTHERPTHR23024:SF135CELL DEATH ASSOCIATED PROTEINcoord: 4..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G20780.1CSPI06G20780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity