CSPI06G06850 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI06G06850
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr6: 5893547 .. 5896808 (+)
RNA-Seq ExpressionCSPI06G06850
SyntenyCSPI06G06850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAGTTGAAAAAAAAGGAAAAGTGAGAAGAGAAGAACAGCTCAGAAAGAAGGAACTTTCTTCATTTATAACCGATCCACATCGACATCTCTGTCCCTCGTATCGACCTATCAGAAATTTTGGAATGAAAAAGGGGAAGAAAAAACTTCCATTATTAACTAATCTCTCCTTCTAAATCCCTCCTTCCTTCTTCTTTCTCGTCATTTCTCTAATCTCAACCACCCATTTCATCTCTTTGATCATCCATTTTCATTCCTTTCTTCATTCATTCTTCACTTTTTCTCGATACCCTTTTCATTTCTATCCCCAATTCGCCGGTTTTCATGGCGAATACCTCTTCCCCTTCAAAACCCATCATCACATTTTGATTCTCTCTCGATTTAATTAATACCCAGTTGGATAGGTCTTTGTTTGTTCTGCAAAAATGGAGCTGGATGGCATCGATGGTATGCCATCCATGGATGTGATGGACGAGGAAGATAAGACCCTTCAACGTCACTGTCAGTTTCCTTCGATTTCCAAGCCTCGAATCAACAATAACGACAATCCCACAACCACAAGTGTCCATGAGCTTCTTGAATGTCCTGTTTGTACCAATTCTATGTATCCCCCAATTCATCAGGTTCCTTTCCCCCAACCCTCTTTTTCTTTTCTTCAATCACTGTTTTGATCCGTTTCTGCTTTCTGTTTTCTGATCTATTTCGTATTGTGGGTCTGTTGTTAGAGGTTCTTGAGATTCTTCTCCTCCATTGGGCCTTTGGTTTTCTCGATCGATCGGTTCTTGTAATGATTATTTTTATAGTTATTTGCATTTTCATTTGCCTTTGCCTTTGTGGAGATTTCTAATCTTTGTGAGTCTAAATTGTTTGCTAAAGCCCTGCCCTTCAATATACTCATAATCATCAAAATGAAGAGCCATTTTGTTGTGTTGCTATCATTTGTTGTGGTTTAGTCAACTTTGTTCAGAAACTGAAATCATGTCCCAATTGTGGCTCTTTTTTAGGTTAGTTTAATTAATACATGGAGGGGAAAATCTGGGATCTCTTTCTCATATTATCTTAAATATCATCAATTGGGGAAGTGGGAATCCTATGTTTGACCTTTAGAGCGACTATCTTAGCGCTGCAAGATCAGGCTTGCATAGGGCAGGTGAATGGAATGGTGAAGGTGAAATTTGAAAAGAATTTACTTCATTGTTTTACCTTCCCCGTTCCAAATCGAGTTCTCAGTGGTACTTGCAGGCTGGAATGGCTAGAATATAGGATTGTCTTGGACTCACAATGCTGGGATCATCTCTTTTTCTATTTCAATTTAGATTGAATATTGTCAAAGGTTTTGCTCTAAATCAATGCTCAGATCATCTCTTTTGCTTATAGCATACGGTTTCATAAGCGAGTTAGAAGGTTGAGGCACTTAGGATAGGCATTGGACATGGTAAATAGATGGTTATATTTTTCAATATAGCATCCTAAAGTTTAGTAATTGATCTAATATTCTGTTTGATTTCTTGTTGACTATGTGGTGCTATGCAACGATGTTAGTTTTGCATTAGGTTAGTACAAACGACAGGTCTCGTCTCTTCTTGTTTGATGTTAGTAACCAATTCCTAAAATTATACTCGTTCTTAAAACATTTTCAATAAAAGTGGTTGTAAAATTAACTTGCTTATGGGTGTGGTTACATTTTCTAAATTTTTAGTCACAAGTTTGTCTGGTCGCATCACATCTCAAAGTTTACTAATGTTAAATTACAATATTGATTTTATATGATTCTCAGCCAATAACTTTAGAAATCATTTTTGCATAATCTACATCAAGTGTTTCTGGAAAAATATTCTAAAATCATTTTTGAATTTCTTAAATGTGATGTTTATTTTCATCCAAAATCACTTGGGAACTTATGTCAAACTCAATCTTAGAACTCCTGGACGATTTTTGAATACTCCCTTCTTGGCTTAGCCTGGATATAGATGTTTATGTGTATGTGTGTATGTCTATTTAACACTATCACGTAGGAATGTCTTGTCTATTTTGGTATTCGCACCACGATAGGTGTGGATTCTTCGAAAGGACAAAACAACTAATTCGTCAATCTTGTGAAATCTCTAGCGTTAAATGATATTGAATCTTCATGAATGAATTTCGGGTAATGACTATCCAGTTTGGGATAGTTGACGAGAAGAAATTGCTGAATGTTTTGAACTTTTTGTTTTGTTTGTGCAGTGTCCCAATGGGCATACCCTCTGTTCTTCGTGTAAAACAAGGGTAGACAACCGTTGCCCGACATGCAGGCAAGAGCTCGGTGATATCAGATGTCTAGCATTAGAAAAAATAGCTGAATCGCTTAAACTGGCTTGTAAATTCTCCACATTTGGGTGCCAGGAGATATTGCCTTACTACAGCAAACTCAAACATGAATCTGCCTGTTACTATAGACCATATACCTGCCCTTATGCTGGATCAGACTGCCCAATTGATGGCAATATTCCTTTCCTTGTTTCCCATCTTCGGGATGATCACAAGGTCGATATGCATTCTGGATGCACGTTTAATCATCGTTATGTAAAGGCTAATCCATGCGAAGTCGAAAACGCCACATGGATGCTAACTGTACGTATCCATTCAACATGATCGAACAGTCGTGGGTGACAATATTCAAGATTATTTTTCACTTATAATGAGCTTCTATTTATGCTCTTATTTCACCTTTGGCTCAGGTCTTCCACTGCTTTGGCCAGTATTTCTGTCTTCATTTTGAAGCCTTTCAGCTGGGTATGGCACCAGTTTACATGGCATTTCTGCGATTCATGGGGGACGAAACCGACGCTCGAAACTTCAGTTACAGCCTCGAGGTTGGTGGAAATGGTAGGAAGCTGATATGGGAAGGCAACCCTCGAAGCATTCGGGACAACCACAAGAAAGTTAGGGACAGCCATGACGGTCTCATTATACAACGAAACATGGCGCTGTTCTTTTCGGGAGGAGAAAGGAAAGAGCTGAAGCTGCGGATCACGGGACGGATTTGGAAAGAACAAAATAAACAGTAAACTTGTTAGAGATTCAACTCTTTATGTATTTGACTGTAATGCATAACATTTGCTTCAACTTTATCCACCAGAAGCCAATCTTGAATCAGTTCATATCTATTCTTCAAATCTAACAGGAAGAAGAAAAAAAAAAGGCCTTAAAACCATTTCCAATTCCAATTCCAATTCCAACCTTCTTGTAATTGAGATTTAGCTTTTACAATGTCAAACATTGAGA

mRNA sequence

GAAGTTGAAAAAAAAGGAAAAGTGAGAAGAGAAGAACAGCTCAGAAAGAAGGAACTTTCTTCATTTATAACCGATCCACATCGACATCTCTGTCCCTCGTATCGACCTATCAGAAATTTTGGAATGAAAAAGGGGAAGAAAAAACTTCCATTATTAACTAATCTCTCCTTCTAAATCCCTCCTTCCTTCTTCTTTCTCGTCATTTCTCTAATCTCAACCACCCATTTCATCTCTTTGATCATCCATTTTCATTCCTTTCTTCATTCATTCTTCACTTTTTCTCGATACCCTTTTCATTTCTATCCCCAATTCGCCGGTTTTCATGGCGAATACCTCTTCCCCTTCAAAACCCATCATCACATTTTGATTCTCTCTCGATTTAATTAATACCCAGTTGGATAGGTCTTTGTTTGTTCTGCAAAAATGGAGCTGGATGGCATCGATGGTATGCCATCCATGGATGTGATGGACGAGGAAGATAAGACCCTTCAACGTCACTGTCAGTTTCCTTCGATTTCCAAGCCTCGAATCAACAATAACGACAATCCCACAACCACAAGTGTCCATGAGCTTCTTGAATGTCCTGTTTGTACCAATTCTATGTATCCCCCAATTCATCAGTGTCCCAATGGGCATACCCTCTGTTCTTCGTGTAAAACAAGGGTAGACAACCGTTGCCCGACATGCAGGCAAGAGCTCGGTGATATCAGATGTCTAGCATTAGAAAAAATAGCTGAATCGCTTAAACTGGCTTGTAAATTCTCCACATTTGGGTGCCAGGAGATATTGCCTTACTACAGCAAACTCAAACATGAATCTGCCTGTTACTATAGACCATATACCTGCCCTTATGCTGGATCAGACTGCCCAATTGATGGCAATATTCCTTTCCTTGTTTCCCATCTTCGGGATGATCACAAGGTCGATATGCATTCTGGATGCACGTTTAATCATCGTTATGTAAAGGCTAATCCATGCGAAGTCGAAAACGCCACATGGATGCTAACTGTCTTCCACTGCTTTGGCCAGTATTTCTGTCTTCATTTTGAAGCCTTTCAGCTGGGTATGGCACCAGTTTACATGGCATTTCTGCGATTCATGGGGGACGAAACCGACGCTCGAAACTTCAGTTACAGCCTCGAGGTTGGTGGAAATGGTAGGAAGCTGATATGGGAAGGCAACCCTCGAAGCATTCGGGACAACCACAAGAAAGTTAGGGACAGCCATGACGGTCTCATTATACAACGAAACATGGCGCTGTTCTTTTCGGGAGGAGAAAGGAAAGAGCTGAAGCTGCGGATCACGGGACGGATTTGGAAAGAACAAAATAAACAGTAAACTTGTTAGAGATTCAACTCTTTATGTATTTGACTGTAATGCATAACATTTGCTTCAACTTTATCCACCAGAAGCCAATCTTGAATCAGTTCATATCTATTCTTCAAATCTAACAGGAAGAAGAAAAAAAAAAGGCCTTAAAACCATTTCCAATTCCAATTCCAATTCCAACCTTCTTGTAATTGAGATTTAGCTTTTACAATGTCAAACATTGAGA

Coding sequence (CDS)

ATGGAGCTGGATGGCATCGATGGTATGCCATCCATGGATGTGATGGACGAGGAAGATAAGACCCTTCAACGTCACTGTCAGTTTCCTTCGATTTCCAAGCCTCGAATCAACAATAACGACAATCCCACAACCACAAGTGTCCATGAGCTTCTTGAATGTCCTGTTTGTACCAATTCTATGTATCCCCCAATTCATCAGTGTCCCAATGGGCATACCCTCTGTTCTTCGTGTAAAACAAGGGTAGACAACCGTTGCCCGACATGCAGGCAAGAGCTCGGTGATATCAGATGTCTAGCATTAGAAAAAATAGCTGAATCGCTTAAACTGGCTTGTAAATTCTCCACATTTGGGTGCCAGGAGATATTGCCTTACTACAGCAAACTCAAACATGAATCTGCCTGTTACTATAGACCATATACCTGCCCTTATGCTGGATCAGACTGCCCAATTGATGGCAATATTCCTTTCCTTGTTTCCCATCTTCGGGATGATCACAAGGTCGATATGCATTCTGGATGCACGTTTAATCATCGTTATGTAAAGGCTAATCCATGCGAAGTCGAAAACGCCACATGGATGCTAACTGTCTTCCACTGCTTTGGCCAGTATTTCTGTCTTCATTTTGAAGCCTTTCAGCTGGGTATGGCACCAGTTTACATGGCATTTCTGCGATTCATGGGGGACGAAACCGACGCTCGAAACTTCAGTTACAGCCTCGAGGTTGGTGGAAATGGTAGGAAGCTGATATGGGAAGGCAACCCTCGAAGCATTCGGGACAACCACAAGAAAGTTAGGGACAGCCATGACGGTCTCATTATACAACGAAACATGGCGCTGTTCTTTTCGGGAGGAGAAAGGAAAGAGCTGAAGCTGCGGATCACGGGACGGATTTGGAAAGAACAAAATAAACAGTAA

Protein sequence

MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQEILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLEVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKEQNKQ*
Homology
BLAST of CSPI06G06850 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 9.0e-146
Identity = 240/314 (76.43%), Postives = 269/314 (85.67%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDN-----------PTTTSVHE 60
           M+LD +D   +MDV D+E+    RH  + S+SK    NN+            PTTTSVHE
Sbjct: 1   MDLDSMDCTSTMDVTDDEEIHQDRH-SYASVSKHHHTNNNTTNVNAAASGLLPTTTSVHE 60

Query: 61  LLECPVCTNSMYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKL 120
           LLECPVCTNSMYPPIHQC NGHTLCS+CK RV NRCPTCRQELGDIRCLALEK+AESL+L
Sbjct: 61  LLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLEL 120

Query: 121 ACKFSTFGCQEILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDM 180
            CK  + GC EI PYYSKLKHE+ C +RPY+CPYAGS+C + G+IPFLV+HLRDDHKVDM
Sbjct: 121 PCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDM 180

Query: 181 HSGCTFNHRYVKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDE 240
           HSGCTFNHRYVK+NP EVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDE
Sbjct: 181 HSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDE 240

Query: 241 TDARNFSYSLEVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKEL 300
           T+ARN++YSLEVGG GRKLIWEG PRS+RD+H+KVRDSHDGLIIQRNMALFFSGG+RKEL
Sbjct: 241 TEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKEL 300

Query: 301 KLRITGRIWKEQNK 304
           KLR+TGRIWKEQ +
Sbjct: 301 KLRVTGRIWKEQQQ 313

BLAST of CSPI06G06850 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 3.5e-134
Identity = 217/258 (84.11%), Postives = 238/258 (92.25%), Query Frame = 0

Query: 45  TSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIA 104
           TSV+ELLECPVCT SMYPPIHQC NGHTLCS+CK RV NRCPTCRQELGDIRCLALEK+A
Sbjct: 56  TSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVA 115

Query: 105 ESLKLACKFSTFGCQEILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDD 164
           ESL+L CKF   GC EI PYYSKLKHES C +RPY+CPYAGS+C I G+IPFLV+HLRDD
Sbjct: 116 ESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDD 175

Query: 165 HKVDMHSGCTFNHRYVKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLR 224
           HKVDMH+G TFNHRYVK+NP EVENATWMLTVFHCFGQYFCLHFEAFQLGM PVYMAFLR
Sbjct: 176 HKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLR 235

Query: 225 FMGDETDARNFSYSLEVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGG 284
           FMGDE DAR++SYSLEVGG+GRKL WEG PRSIRD+H+KVRDS+DGLIIQRNMALFFSGG
Sbjct: 236 FMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGG 295

Query: 285 ERKELKLRITGRIWKEQN 303
           +RKELKLR+TG+IWKEQ+
Sbjct: 296 DRKELKLRVTGKIWKEQH 313

BLAST of CSPI06G06850 vs. ExPASy Swiss-Prot
Match: Q8S3N1 (E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=1 SV=2)

HSP 1 Score: 478.8 bits (1231), Expect = 4.6e-134
Identity = 222/291 (76.29%), Postives = 250/291 (85.91%), Query Frame = 0

Query: 11  SMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSMYPPIHQCPNG 70
           S+D + ++D+  Q+H QF S              TSV+ELLECPVCTNSMYPPIHQC NG
Sbjct: 5   SIDSVIDDDEIHQKH-QFSSTKSQGGATVVISPATSVYELLECPVCTNSMYPPIHQCHNG 64

Query: 71  HTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQEILPYYSKLKH 130
           HTLCS+CK+RV NRCPTCRQELGDIRCLALEK+AESL+L CK+   GC  I PYYSKLKH
Sbjct: 65  HTLCSTCKSRVHNRCPTCRQELGDIRCLALEKVAESLELPCKYYNLGCLGIFPYYSKLKH 124

Query: 131 ESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENA 190
           ES C +RPY+CPYAGS+C   G+I FLV+HLRDDHKVDMH+GCTFNHRYVK+NP EVENA
Sbjct: 125 ESQCNFRPYSCPYAGSECAAVGDITFLVAHLRDDHKVDMHTGCTFNHRYVKSNPREVENA 184

Query: 191 TWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLEVGGNGRKLIW 250
           TWMLTVF CFGQYFCLHFEAFQLGMAPVYMAFLRFMGDE DARN++YSLEVGG+GRK  W
Sbjct: 185 TWMLTVFQCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTW 244

Query: 251 EGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKEQ 302
           EG PRS+RD+H+KVRDSHDGLIIQRNMALFFSGG++KELKLR+TGRIWKEQ
Sbjct: 245 EGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDKKELKLRVTGRIWKEQ 294

BLAST of CSPI06G06850 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 1.2e-129
Identity = 206/258 (79.84%), Postives = 234/258 (90.70%), Query Frame = 0

Query: 44  TTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKI 103
           ++SV ELLECPVC ++MYPPIHQC NGHTLCS CK RV NRCPTCR ELG+IRCLALEK+
Sbjct: 44  SSSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNIRCLALEKV 103

Query: 104 AESLKLACKFSTFGCQEILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRD 163
           A SL+L CK+  FGC  I PYY KLKHES C YRPY+CPYAGS+C + G+IP+LV+HL+D
Sbjct: 104 AASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIPYLVNHLKD 163

Query: 164 DHKVDMHSGCTFNHRYVKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFL 223
           DHKVDMH+GCTFNHRYVK+NP EVENATWMLTVF CFGQYFCLHFEAFQLGMAPVY+AFL
Sbjct: 164 DHKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGMAPVYIAFL 223

Query: 224 RFMGDETDARNFSYSLEVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSG 283
           RFMGD+ +A+N+SYSLEVGG GRK+IW+G PRSIRD+H+KVRDS+DGLIIQRNMALFFSG
Sbjct: 224 RFMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQRNMALFFSG 283

Query: 284 GERKELKLRITGRIWKEQ 302
           GERKELKLR+TGRIWKEQ
Sbjct: 284 GERKELKLRVTGRIWKEQ 301

BLAST of CSPI06G06850 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 3.0e-125
Identity = 206/290 (71.03%), Postives = 238/290 (82.07%), Query Frame = 0

Query: 15  MDEEDKTLQRHCQFPSISKP---RINNNDNPTTTSVHELLECPVCTNSMYPPIHQCPNGH 74
           MD E KT +        +KP    I      +   V+ELLECPVCTN MYPPIHQCPNGH
Sbjct: 19  MDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGH 78

Query: 75  TLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQEILPYYSKLKHE 134
           TLCS+CK RV N CPTCR ELG+IRCLALEK+AESL++ C++   GC +I PYYSKLKHE
Sbjct: 79  TLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHE 138

Query: 135 SACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAT 194
             C +RPYTCPYAGS+C + G+IP LV HL+DDHKVDMH GCTFNHRYVK+NP EVENAT
Sbjct: 139 QHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENAT 198

Query: 195 WMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLEVGGNGRKLIWE 254
           WMLTVF+CFG+ FCLHFEAFQLGMAPVYMAFLRFMGDE +A+ FSYSLEVG +GRKL W+
Sbjct: 199 WMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQ 258

Query: 255 GNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKEQ 302
           G PRSIRD+H+KVRDS DGLII RN+AL+FSGG+R+ELKLR+TGRIWKE+
Sbjct: 259 GIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of CSPI06G06850 vs. ExPASy TrEMBL
Match: A0A0A0KF08 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G087840 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 6.0e-185
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60
           MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM
Sbjct: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60

Query: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120
           YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE
Sbjct: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120

Query: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180
           ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV
Sbjct: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180

Query: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240
           KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE
Sbjct: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240

Query: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300
           VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE
Sbjct: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300

Query: 301 QNKQ 305
           QNKQ
Sbjct: 301 QNKQ 304

BLAST of CSPI06G06850 vs. ExPASy TrEMBL
Match: A0A5D3DLU6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001410 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.7e-184
Identity = 303/304 (99.67%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60
           MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM
Sbjct: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60

Query: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120
           YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE
Sbjct: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120

Query: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180
           ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV
Sbjct: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180

Query: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240
           KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDE+DARNFSYSLE
Sbjct: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDESDARNFSYSLE 240

Query: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300
           VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE
Sbjct: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300

Query: 301 QNKQ 305
           QNKQ
Sbjct: 301 QNKQ 304

BLAST of CSPI06G06850 vs. ExPASy TrEMBL
Match: A0A1S3CBH3 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498939 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.7e-184
Identity = 303/304 (99.67%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60
           MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM
Sbjct: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60

Query: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120
           YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE
Sbjct: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120

Query: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180
           ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV
Sbjct: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180

Query: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240
           KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDE+DARNFSYSLE
Sbjct: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDESDARNFSYSLE 240

Query: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300
           VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE
Sbjct: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300

Query: 301 QNKQ 305
           QNKQ
Sbjct: 301 QNKQ 304

BLAST of CSPI06G06850 vs. ExPASy TrEMBL
Match: A0A6J1JVF7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488681 PE=3 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.1e-181
Identity = 296/304 (97.37%), Postives = 302/304 (99.34%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60
           MELDGI+G+PSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM
Sbjct: 1   MELDGIEGVPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60

Query: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120
           YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKF TFGC E
Sbjct: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCPE 120

Query: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180
           ILPYYSKLKHESACY+RPYTCPYAGSDCP+DG+IPFLVSHLRDDHKVDMHSGCTFNHRYV
Sbjct: 121 ILPYYSKLKHESACYFRPYTCPYAGSDCPVDGDIPFLVSHLRDDHKVDMHSGCTFNHRYV 180

Query: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240
           KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARN+SYSLE
Sbjct: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNYSYSLE 240

Query: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300
           VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE
Sbjct: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300

Query: 301 QNKQ 305
           QNKQ
Sbjct: 301 QNKQ 304

BLAST of CSPI06G06850 vs. ExPASy TrEMBL
Match: A0A6J1ENP4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111436299 PE=3 SV=1)

HSP 1 Score: 644.0 bits (1660), Expect = 3.1e-181
Identity = 296/304 (97.37%), Postives = 302/304 (99.34%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60
           MELDGI+G+PSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM
Sbjct: 1   MELDGIEGVPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60

Query: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120
           YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKF TFGC E
Sbjct: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCPE 120

Query: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180
           ILPYYSKLKHESACY+RPYTCPYAGSDCP+DG+IPFLVSHLRDDHKVDMHSGCTFNHRYV
Sbjct: 121 ILPYYSKLKHESACYFRPYTCPYAGSDCPVDGDIPFLVSHLRDDHKVDMHSGCTFNHRYV 180

Query: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240
           KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARN+SYSLE
Sbjct: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNYSYSLE 240

Query: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300
           VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE
Sbjct: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300

Query: 301 QNKQ 305
           QNKQ
Sbjct: 301 QNKQ 304

BLAST of CSPI06G06850 vs. NCBI nr
Match: XP_004140587.1 (E3 ubiquitin-protein ligase SINAT3 [Cucumis sativus] >KGN46356.1 hypothetical protein Csa_004964 [Cucumis sativus])

HSP 1 Score: 656.4 bits (1692), Expect = 1.2e-184
Identity = 304/304 (100.00%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60
           MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM
Sbjct: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60

Query: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120
           YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE
Sbjct: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120

Query: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180
           ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV
Sbjct: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180

Query: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240
           KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE
Sbjct: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240

Query: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300
           VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE
Sbjct: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300

Query: 301 QNKQ 305
           QNKQ
Sbjct: 301 QNKQ 304

BLAST of CSPI06G06850 vs. NCBI nr
Match: XP_008459990.1 (PREDICTED: E3 ubiquitin-protein ligase SINAT3-like [Cucumis melo] >KAA0039905.1 E3 ubiquitin-protein ligase SINAT3-like [Cucumis melo var. makuwa] >TYK24595.1 E3 ubiquitin-protein ligase SINAT3-like [Cucumis melo var. makuwa])

HSP 1 Score: 654.8 bits (1688), Expect = 3.6e-184
Identity = 303/304 (99.67%), Postives = 304/304 (100.00%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60
           MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM
Sbjct: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60

Query: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120
           YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE
Sbjct: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120

Query: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180
           ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV
Sbjct: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180

Query: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240
           KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDE+DARNFSYSLE
Sbjct: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDESDARNFSYSLE 240

Query: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300
           VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE
Sbjct: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300

Query: 301 QNKQ 305
           QNKQ
Sbjct: 301 QNKQ 304

BLAST of CSPI06G06850 vs. NCBI nr
Match: XP_022929797.1 (E3 ubiquitin-protein ligase SINAT3-like [Cucurbita moschata] >XP_022992340.1 E3 ubiquitin-protein ligase SINAT3-like [Cucurbita maxima] >XP_023548870.1 E3 ubiquitin-protein ligase SINAT3-like [Cucurbita pepo subsp. pepo] >KAG6575290.1 E3 ubiquitin-protein ligase SINAT3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013845.1 E3 ubiquitin-protein ligase SINAT3 [Cucurbita argyrosperma subsp. argyrosperma] >KAG6575314.1 E3 ubiquitin-protein ligase SINAT3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 644.0 bits (1660), Expect = 6.3e-181
Identity = 296/304 (97.37%), Postives = 302/304 (99.34%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60
           MELDGI+G+PSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM
Sbjct: 1   MELDGIEGVPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60

Query: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120
           YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKF TFGC E
Sbjct: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCPE 120

Query: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180
           ILPYYSKLKHESACY+RPYTCPYAGSDCP+DG+IPFLVSHLRDDHKVDMHSGCTFNHRYV
Sbjct: 121 ILPYYSKLKHESACYFRPYTCPYAGSDCPVDGDIPFLVSHLRDDHKVDMHSGCTFNHRYV 180

Query: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240
           KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARN+SYSLE
Sbjct: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNYSYSLE 240

Query: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300
           VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE
Sbjct: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300

Query: 301 QNKQ 305
           QNKQ
Sbjct: 301 QNKQ 304

BLAST of CSPI06G06850 vs. NCBI nr
Match: XP_038876068.1 (E3 ubiquitin-protein ligase SINAT3-like [Benincasa hispida])

HSP 1 Score: 643.7 bits (1659), Expect = 8.3e-181
Identity = 296/304 (97.37%), Postives = 302/304 (99.34%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60
           MELDGI+G+PSMDVMDEEDKTLQRHCQFPSISKPRI+NNDNPTTTSVHELLECPVCTNSM
Sbjct: 1   MELDGIEGVPSMDVMDEEDKTLQRHCQFPSISKPRIHNNDNPTTTSVHELLECPVCTNSM 60

Query: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120
           YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKF TFGCQE
Sbjct: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTFGCQE 120

Query: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180
           ILPYYSKLKHESACYYRPY CPYAGSDCP+DG+IPFLVSHLRDDHKVDMHSGCTFNHRYV
Sbjct: 121 ILPYYSKLKHESACYYRPYICPYAGSDCPVDGDIPFLVSHLRDDHKVDMHSGCTFNHRYV 180

Query: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240
           KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARN+SYSLE
Sbjct: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNYSYSLE 240

Query: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300
           VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE
Sbjct: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300

Query: 301 QNKQ 305
           QNKQ
Sbjct: 301 QNKQ 304

BLAST of CSPI06G06850 vs. NCBI nr
Match: XP_022157793.1 (E3 ubiquitin-protein ligase SINAT3-like [Momordica charantia])

HSP 1 Score: 637.1 bits (1642), Expect = 7.7e-179
Identity = 291/304 (95.72%), Postives = 301/304 (99.01%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60
           MELDGI+G+PSMDVMDEED TLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM
Sbjct: 1   MELDGIEGVPSMDVMDEEDTTLQRHCQFPSISKPRINNNDNPTTTSVHELLECPVCTNSM 60

Query: 61  YPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQE 120
           YPPIHQCPNGHTLCS+CKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKF T+GC E
Sbjct: 61  YPPIHQCPNGHTLCSTCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFCTYGCPE 120

Query: 121 ILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYV 180
           ILPYYSKLKHE+ACY+RPYTCPYAGSDCP+DG+IPFLVSHLRDDHKVDMHSGCTFNHRYV
Sbjct: 121 ILPYYSKLKHEAACYFRPYTCPYAGSDCPVDGDIPFLVSHLRDDHKVDMHSGCTFNHRYV 180

Query: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLE 240
           KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARN+SYSLE
Sbjct: 181 KANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNYSYSLE 240

Query: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKE 300
           VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGE+KELKLRITGRIWKE
Sbjct: 241 VGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGEKKELKLRITGRIWKE 300

Query: 301 QNKQ 305
           QNKQ
Sbjct: 301 QNKQ 304

BLAST of CSPI06G06850 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 517.7 bits (1332), Expect = 6.4e-147
Identity = 240/314 (76.43%), Postives = 269/314 (85.67%), Query Frame = 0

Query: 1   MELDGIDGMPSMDVMDEEDKTLQRHCQFPSISKPRINNNDN-----------PTTTSVHE 60
           M+LD +D   +MDV D+E+    RH  + S+SK    NN+            PTTTSVHE
Sbjct: 1   MDLDSMDCTSTMDVTDDEEIHQDRH-SYASVSKHHHTNNNTTNVNAAASGLLPTTTSVHE 60

Query: 61  LLECPVCTNSMYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKL 120
           LLECPVCTNSMYPPIHQC NGHTLCS+CK RV NRCPTCRQELGDIRCLALEK+AESL+L
Sbjct: 61  LLECPVCTNSMYPPIHQCHNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLEL 120

Query: 121 ACKFSTFGCQEILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDM 180
            CK  + GC EI PYYSKLKHE+ C +RPY+CPYAGS+C + G+IPFLV+HLRDDHKVDM
Sbjct: 121 PCKHMSLGCPEIFPYYSKLKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDM 180

Query: 181 HSGCTFNHRYVKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDE 240
           HSGCTFNHRYVK+NP EVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDE
Sbjct: 181 HSGCTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDE 240

Query: 241 TDARNFSYSLEVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKEL 300
           T+ARN++YSLEVGG GRKLIWEG PRS+RD+H+KVRDSHDGLIIQRNMALFFSGG+RKEL
Sbjct: 241 TEARNYNYSLEVGGYGRKLIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKEL 300

Query: 301 KLRITGRIWKEQNK 304
           KLR+TGRIWKEQ +
Sbjct: 301 KLRVTGRIWKEQQQ 313

BLAST of CSPI06G06850 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 479.2 bits (1232), Expect = 2.5e-135
Identity = 217/258 (84.11%), Postives = 238/258 (92.25%), Query Frame = 0

Query: 45  TSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEKIA 104
           TSV+ELLECPVCT SMYPPIHQC NGHTLCS+CK RV NRCPTCRQELGDIRCLALEK+A
Sbjct: 56  TSVYELLECPVCTYSMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVA 115

Query: 105 ESLKLACKFSTFGCQEILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDD 164
           ESL+L CKF   GC EI PYYSKLKHES C +RPY+CPYAGS+C I G+IPFLV+HLRDD
Sbjct: 116 ESLELPCKFYNLGCPEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDD 175

Query: 165 HKVDMHSGCTFNHRYVKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLR 224
           HKVDMH+G TFNHRYVK+NP EVENATWMLTVFHCFGQYFCLHFEAFQLGM PVYMAFLR
Sbjct: 176 HKVDMHAGSTFNHRYVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLR 235

Query: 225 FMGDETDARNFSYSLEVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGG 284
           FMGDE DAR++SYSLEVGG+GRKL WEG PRSIRD+H+KVRDS+DGLIIQRNMALFFSGG
Sbjct: 236 FMGDEEDARSYSYSLEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGG 295

Query: 285 ERKELKLRITGRIWKEQN 303
           +RKELKLR+TG+IWKEQ+
Sbjct: 296 DRKELKLRVTGKIWKEQH 313

BLAST of CSPI06G06850 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 449.5 bits (1155), Expect = 2.1e-126
Identity = 206/290 (71.03%), Postives = 238/290 (82.07%), Query Frame = 0

Query: 15  MDEEDKTLQRHCQFPSISKP---RINNNDNPTTTSVHELLECPVCTNSMYPPIHQCPNGH 74
           MD E KT +        +KP    I      +   V+ELLECPVCTN MYPPIHQCPNGH
Sbjct: 19  MDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLECPVCTNLMYPPIHQCPNGH 78

Query: 75  TLCSSCKTRVDNRCPTCRQELGDIRCLALEKIAESLKLACKFSTFGCQEILPYYSKLKHE 134
           TLCS+CK RV N CPTCR ELG+IRCLALEK+AESL++ C++   GC +I PYYSKLKHE
Sbjct: 79  TLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQNLGCHDIFPYYSKLKHE 138

Query: 135 SACYYRPYTCPYAGSDCPIDGNIPFLVSHLRDDHKVDMHSGCTFNHRYVKANPCEVENAT 194
             C +RPYTCPYAGS+C + G+IP LV HL+DDHKVDMH GCTFNHRYVK+NP EVENAT
Sbjct: 139 QHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGCTFNHRYVKSNPHEVENAT 198

Query: 195 WMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETDARNFSYSLEVGGNGRKLIWE 254
           WMLTVF+CFG+ FCLHFEAFQLGMAPVYMAFLRFMGDE +A+ FSYSLEVG +GRKL W+
Sbjct: 199 WMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKKFSYSLEVGAHGRKLTWQ 258

Query: 255 GNPRSIRDNHKKVRDSHDGLIIQRNMALFFSGGERKELKLRITGRIWKEQ 302
           G PRSIRD+H+KVRDS DGLII RN+AL+FSGG+R+ELKLR+TGRIWKE+
Sbjct: 259 GIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRVTGRIWKEE 308

BLAST of CSPI06G06850 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 443.4 bits (1139), Expect = 1.5e-124
Identity = 195/259 (75.29%), Postives = 227/259 (87.64%), Query Frame = 0

Query: 43  TTTSVHELLECPVCTNSMYPPIHQCPNGHTLCSSCKTRVDNRCPTCRQELGDIRCLALEK 102
           ++  V+ELLECPVCTN MYPPIHQCPNGHTLCSSCK RV N CPTCR ELG+IRCLALEK
Sbjct: 47  SSNGVYELLECPVCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEK 106

Query: 103 IAESLKLACKFSTFGCQEILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFLVSHLR 162
           +AESL++ C++   GCQ+I PYYSKLKHE  C +R Y+CPYAGS+C + G+IP LV HL+
Sbjct: 107 VAESLEVPCRYQNLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLK 166

Query: 163 DDHKVDMHSGCTFNHRYVKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAF 222
           DDHKVDMH GCTFNHRYVK+NP EVENATWMLTVF+CFG+ FCLHFEAFQLGMAPVYMAF
Sbjct: 167 DDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAF 226

Query: 223 LRFMGDETDARNFSYSLEVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNMALFFS 282
           LRFMGDE +A+ FSYSLEVG + RKL W+G PRSIRD+H+KVRDS DGLII RN+AL+FS
Sbjct: 227 LRFMGDENEAKKFSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFS 286

Query: 283 GGERKELKLRITGRIWKEQ 302
           G +++ELKLR+TGRIWKE+
Sbjct: 287 GSDKEELKLRVTGRIWKEE 305

BLAST of CSPI06G06850 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 367.1 bits (941), Expect = 1.4e-101
Identity = 165/204 (80.88%), Postives = 185/204 (90.69%), Query Frame = 0

Query: 98  LALEKIAESLKLACKFSTFGCQEILPYYSKLKHESACYYRPYTCPYAGSDCPIDGNIPFL 157
           +ALEK+AESL+L CK+   GC  I PYYSKLKHES C +RPY+CPYAGS+C   G+I FL
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 158 VSHLRDDHKVDMHSGCTFNHRYVKANPCEVENATWMLTVFHCFGQYFCLHFEAFQLGMAP 217
           V+HLRDDHKVDMH+GCTFNHRYVK+NP EVENATWMLTVF CFGQYFCLHFEAFQLGMAP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 218 VYMAFLRFMGDETDARNFSYSLEVGGNGRKLIWEGNPRSIRDNHKKVRDSHDGLIIQRNM 277
           VYMAFLRFMGDE DARN++YSLEVGG+GRK  WEG PRS+RD+H+KVRDSHDGLIIQRNM
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 278 ALFFSGGERKELKLRITGRIWKEQ 302
           ALFFSGG++KELKLR+TGRIWKEQ
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q84JL39.0e-14676.43E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=... [more]
Q9STN83.5e-13484.11E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=... [more]
Q8S3N14.6e-13476.29E3 ubiquitin-protein ligase SINAT5 OS=Arabidopsis thaliana OX=3702 GN=SINAT5 PE=... [more]
Q10L911.2e-12979.84E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS... [more]
Q9M2P43.0e-12571.03E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=... [more]
Match NameE-valueIdentityDescription
A0A0A0KF086.0e-185100.00RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G087840 PE... [more]
A0A5D3DLU61.7e-18499.67RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CBH31.7e-18499.67RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498939 PE=3 ... [more]
A0A6J1JVF73.1e-18197.37RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111488681 P... [more]
A0A6J1ENP43.1e-18197.37RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111436299... [more]
Match NameE-valueIdentityDescription
XP_004140587.11.2e-184100.00E3 ubiquitin-protein ligase SINAT3 [Cucumis sativus] >KGN46356.1 hypothetical pr... [more]
XP_008459990.13.6e-18499.67PREDICTED: E3 ubiquitin-protein ligase SINAT3-like [Cucumis melo] >KAA0039905.1 ... [more]
XP_022929797.16.3e-18197.37E3 ubiquitin-protein ligase SINAT3-like [Cucurbita moschata] >XP_022992340.1 E3 ... [more]
XP_038876068.18.3e-18197.37E3 ubiquitin-protein ligase SINAT3-like [Benincasa hispida][more]
XP_022157793.17.7e-17995.72E3 ubiquitin-protein ligase SINAT3-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT3G61790.16.4e-14776.43Protein with RING/U-box and TRAF-like domains [more]
AT4G27880.12.5e-13584.11Protein with RING/U-box and TRAF-like domains [more]
AT3G58040.12.1e-12671.03seven in absentia of Arabidopsis 2 [more]
AT2G41980.11.5e-12475.29Protein with RING/U-box and TRAF-like domains [more]
AT5G53360.11.4e-10180.88TRAF-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 101..161
e-value: 1.6E-15
score: 59.0
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 47..95
e-value: 2.8E-7
score: 31.9
IPR008974TRAF-likeGENE3D2.60.210.10Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain Acoord: 165..294
e-value: 2.2E-38
score: 133.0
IPR018121Seven-in-absentia protein, TRAF-like domainPFAMPF03145Sinacoord: 95..294
e-value: 1.5E-77
score: 260.1
NoneNo IPR availablePANTHERPTHR10315:SF125UBIQUITIN LIGASE SINAT3coord: 9..302
NoneNo IPR availablePANTHERPTHR10315E3 UBIQUITIN PROTEIN LIGASE SIAHcoord: 9..302
NoneNo IPR availableCDDcd03829Sinacoord: 167..295
e-value: 2.16957E-65
score: 199.535
NoneNo IPR availableCDDcd16571RING-HC_SIAHscoord: 51..89
e-value: 1.70639E-14
score: 64.2282
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 47..111
NoneNo IPR availableSUPERFAMILY49599TRAF domain-likecoord: 106..298
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 53..89
score: 9.877997
IPR013010Zinc finger, SIAH-typePROSITEPS51081ZF_SIAHcoord: 106..166
score: 13.810078

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI06G06850.1CSPI06G06850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0007275 multicellular organism development
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005515 protein binding
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding