CSPI05G28380 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G28380
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionRING-type E3 ubiquitin transferase
LocationChr5: 26825348 .. 26827745 (-)
RNA-Seq ExpressionCSPI05G28380
SyntenyCSPI05G28380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAAATCAAAACAATAAATTCGAGCTTCTTGTTCTTCTTCTTCTATCTGCTTCCATGGCAGAGTATTCAAAGCTAATTAACCAAATAATGATGATGAAGAAGAAGGAATTAGCATATTCTATTTTATCCCAAAACACTCAGATTTTTTCTTCATCACTTACTTTGGGATTTACTTAACTTTACTCTTTCTCTCGATTCTACTGATATTCCTCAAACCCCAAATTCTCTCTCTTCGATTCCATTCTGTTGAGACCTCCACGGGAATTAGTCCACCGTTCACCGCTCTTCTTCAGCATTCCCATCCGCTCTTTCCCCTTTACCGGACTTCTCACTAAATTATGGAGCTTCATCAACAATTCATGGACCCGTTTGATTAACGTCGTCGTCGTCGTCTTCTTCTTTCCGTTTTCGTTAAAAAGCTCTTTTAAGGGTTTTCTTCTTTCTTGCAGAGGGATTTGATTTCTGAATCTGAGATTTGTTAATGGAGTTTCAAGAAGGTTTGATGAAGTTTATGTTTCATGAAGATGTTAAACCCAACTGGGTTTTTGGGAAGGATTCTCCATCTGTTGCTGTTTCGGAGATAAGTAGTACTCCTAGACGGAAATGGCGGATCTTCAATCGGTCTTCTTCTTCACCATTTCCGTCAAAACCAAAACCACCCCATTTTAAGGAAATACCTAAAGAATTGCTCTGTCCGATTACTGGGTCTTTAATGGCGGATCCTGTGATTGTTTCCTCTGGTCATACTTTTGAAGCTGCTTGTGTTCAGGTTTGTAAAGATTTAGGGGTGAAACCGACTTTGTTAGATGGTTCTAAGCCGGATTTCTCATCTGTGATTCCAAATCTGGCTCTTAAATCCACCATTTTCAATTGGTGTAAAAATTCCTCTTCTGAACCTCCTCAACCCCTTGATTTCTCCTCTGCTGAAAAGCTTGTTCGTAAATTTGTCGCAGCTCATAGTAAAAGCGATGAGGAATTGATTCAAGGGGTTGCTGAGACTCCTGTGGTTCGATTCAACCATGCCGCCACTGAAGTTGCTCGTCGAAGTTCTCATTTTCATTCGAGTTCCGATGAATCTGTAAGCGCCGTTGTTCCTACTCTGCCTCTTCCTCTTGCGATTCGCCCGAGTTGCTGTTCTTCGTCTTCTTCCTCTGATAACGAGATCATTGGTACTCTTAACTTGCCTGAAGAGGAAGAGATCGTCGTGAAGCTTAAAAGTTCTCAGGTTATTGAGATTGAAGAGGCTGTGACGACGTTGAGAAAAATCACGAGAACTCGAGAGGACAGTAGAGTTCATCTCTGTTCTCCTATGATTCTCTCTGCTTTGCGATCTCTCATCGTCTCAAGGTACTCTGGCGTTCAAGTGAATTCCGTTGCGGCGCTTGTAAATCTATCTTTAGAGAATTTGAACAAGGTGAAGATCGTGCGGTCGGGGATTCTCCCTAATTTGATCGATGTTCTGAAAGGGGGTTCGCCGGAGGTTCAAGAACATGCTGCCGGCGCCATTTTCAGCTTAGCTCTCGAAGATAACAACAAGACGGCGATAGGCGTATTGGGTGCTCTACCACCATTGATAAGGCTGTTGTTATCCAACTCGGAGCAAACTCGGCACGACTCAGCTCTTGCTCTTTACCATTTATCCCACGTTCAGAGCAACCGTTCGAAGTTAGTGAAACTTGGGTCGGTGCCGATACTTCTCGGGATGGTGAAATCCCGTCACATGGCTGGCCGTATTTTGCTGACTTTGTGTAATTTAGCAGCTTGTTTTGAGGGCCGGGCGGCGTTGTTAGACTCCGGCGCGGTGGAGTGTTTGGTGGGGATGTTGAGAGAAAACGAGTTGGACTCTGAATCAACTAGGGAGAGTTGTGTGGCGGTTCTGTTTGGACTGAGTTTCGGCGGATTGAGGTTCAAAGGTCTGGCAAAAACCGCCGGAGCAATGGATGTTTTTATGGCAGTGGAGAAAAATGGGAGTGAAAGATCAAAAGAGAAGGTGAAGAGGATGATGGAGTATATGAAAGCCAGAGATGAAGAAGCTGAGGATGTGAATTGGGAGGAATTGCTCGACTCAGGTTGTTTCGGTAGCCGAAGTCGGTGTCGACTTGGTGCCGGAATGGACAGATCCACCGCAAACTCATCAGAATTCTGAACAGAACCCATTTGAATTTGTAAACTTGGGATTTTTTTTTTCTTTTTTGTTTATCATTTGGGTTGTGAATATTAGTGAAGAACAAACAACTTTTGAATTTCATTTGATTACTCTGTTTTCTTCTCTTTCATCTTCATCTGTTTCCCTTTTTTCTCTTTTCCAGATGAAGATGCCAAATAAAAAGCCTTCTAAAGAAGCAACCTCAAAATTTTGTATTGTGAATTTGTGTATTGTGATTCAGTTACAATCTTC

mRNA sequence

ATAAATCAAAACAATAAATTCGAGCTTCTTGTTCTTCTTCTTCTATCTGCTTCCATGGCAGAGTATTCAAAGCTAATTAACCAAATAATGATGATGAAGAAGAAGGAATTAGCATATTCTATTTTATCCCAAAACACTCAGATTTTTTCTTCATCACTTACTTTGGGATTTACTTAACTTTACTCTTTCTCTCGATTCTACTGATATTCCTCAAACCCCAAATTCTCTCTCTTCGATTCCATTCTGTTGAGACCTCCACGGGAATTAGTCCACCGTTCACCGCTCTTCTTCAGCATTCCCATCCGCTCTTTCCCCTTTACCGGACTTCTCACTAAATTATGGAGCTTCATCAACAATTCATGGACCCGTTTGATTAACGTCGTCGTCGTCGTCTTCTTCTTTCCGTTTTCGTTAAAAAGCTCTTTTAAGGGTTTTCTTCTTTCTTGCAGAGGGATTTGATTTCTGAATCTGAGATTTGTTAATGGAGTTTCAAGAAGGTTTGATGAAGTTTATGTTTCATGAAGATGTTAAACCCAACTGGGTTTTTGGGAAGGATTCTCCATCTGTTGCTGTTTCGGAGATAAGTAGTACTCCTAGACGGAAATGGCGGATCTTCAATCGGTCTTCTTCTTCACCATTTCCGTCAAAACCAAAACCACCCCATTTTAAGGAAATACCTAAAGAATTGCTCTGTCCGATTACTGGGTCTTTAATGGCGGATCCTGTGATTGTTTCCTCTGGTCATACTTTTGAAGCTGCTTGTGTTCAGGTTTGTAAAGATTTAGGGGTGAAACCGACTTTGTTAGATGGTTCTAAGCCGGATTTCTCATCTGTGATTCCAAATCTGGCTCTTAAATCCACCATTTTCAATTGGTGTAAAAATTCCTCTTCTGAACCTCCTCAACCCCTTGATTTCTCCTCTGCTGAAAAGCTTGTTCGTAAATTTGTCGCAGCTCATAGTAAAAGCGATGAGGAATTGATTCAAGGGGTTGCTGAGACTCCTGTGGTTCGATTCAACCATGCCGCCACTGAAGTTGCTCGTCGAAGTTCTCATTTTCATTCGAGTTCCGATGAATCTGTAAGCGCCGTTGTTCCTACTCTGCCTCTTCCTCTTGCGATTCGCCCGAGTTGCTGTTCTTCGTCTTCTTCCTCTGATAACGAGATCATTGGTACTCTTAACTTGCCTGAAGAGGAAGAGATCGTCGTGAAGCTTAAAAGTTCTCAGGTTATTGAGATTGAAGAGGCTGTGACGACGTTGAGAAAAATCACGAGAACTCGAGAGGACAGTAGAGTTCATCTCTGTTCTCCTATGATTCTCTCTGCTTTGCGATCTCTCATCGTCTCAAGGTACTCTGGCGTTCAAGTGAATTCCGTTGCGGCGCTTGTAAATCTATCTTTAGAGAATTTGAACAAGGTGAAGATCGTGCGGTCGGGGATTCTCCCTAATTTGATCGATGTTCTGAAAGGGGGTTCGCCGGAGGTTCAAGAACATGCTGCCGGCGCCATTTTCAGCTTAGCTCTCGAAGATAACAACAAGACGGCGATAGGCGTATTGGGTGCTCTACCACCATTGATAAGGCTGTTGTTATCCAACTCGGAGCAAACTCGGCACGACTCAGCTCTTGCTCTTTACCATTTATCCCACGTTCAGAGCAACCGTTCGAAGTTAGTGAAACTTGGGTCGGTGCCGATACTTCTCGGGATGGTGAAATCCCGTCACATGGCTGGCCGTATTTTGCTGACTTTGTGTAATTTAGCAGCTTGTTTTGAGGGCCGGGCGGCGTTGTTAGACTCCGGCGCGGTGGAGTGTTTGGTGGGGATGTTGAGAGAAAACGAGTTGGACTCTGAATCAACTAGGGAGAGTTGTGTGGCGGTTCTGTTTGGACTGAGTTTCGGCGGATTGAGGTTCAAAGGTCTGGCAAAAACCGCCGGAGCAATGGATGTTTTTATGGCAGTGGAGAAAAATGGGAGTGAAAGATCAAAAGAGAAGGTGAAGAGGATGATGGAGTATATGAAAGCCAGAGATGAAGAAGCTGAGGATGTGAATTGGGAGGAATTGCTCGACTCAGGTTGTTTCGGTAGCCGAAGTCGGTGTCGACTTGGTGCCGGAATGGACAGATCCACCGCAAACTCATCAGAATTCTGAACAGAACCCATTTGAATTTGTAAACTTGGGATTTTTTTTTTCTTTTTTGTTTATCATTTGGGTTGTGAATATTAGTGAAGAACAAACAACTTTTGAATTTCATTTGATTACTCTGTTTTCTTCTCTTTCATCTTCATCTGTTTCCCTTTTTTCTCTTTTCCAGATGAAGATGCCAAATAAAAAGCCTTCTAAAGAAGCAACCTCAAAATTTTGTATTGTGAATTTGTGTATTGTGATTCAGTTACAATCTTC

Coding sequence (CDS)

ATGGAGTTTCAAGAAGGTTTGATGAAGTTTATGTTTCATGAAGATGTTAAACCCAACTGGGTTTTTGGGAAGGATTCTCCATCTGTTGCTGTTTCGGAGATAAGTAGTACTCCTAGACGGAAATGGCGGATCTTCAATCGGTCTTCTTCTTCACCATTTCCGTCAAAACCAAAACCACCCCATTTTAAGGAAATACCTAAAGAATTGCTCTGTCCGATTACTGGGTCTTTAATGGCGGATCCTGTGATTGTTTCCTCTGGTCATACTTTTGAAGCTGCTTGTGTTCAGGTTTGTAAAGATTTAGGGGTGAAACCGACTTTGTTAGATGGTTCTAAGCCGGATTTCTCATCTGTGATTCCAAATCTGGCTCTTAAATCCACCATTTTCAATTGGTGTAAAAATTCCTCTTCTGAACCTCCTCAACCCCTTGATTTCTCCTCTGCTGAAAAGCTTGTTCGTAAATTTGTCGCAGCTCATAGTAAAAGCGATGAGGAATTGATTCAAGGGGTTGCTGAGACTCCTGTGGTTCGATTCAACCATGCCGCCACTGAAGTTGCTCGTCGAAGTTCTCATTTTCATTCGAGTTCCGATGAATCTGTAAGCGCCGTTGTTCCTACTCTGCCTCTTCCTCTTGCGATTCGCCCGAGTTGCTGTTCTTCGTCTTCTTCCTCTGATAACGAGATCATTGGTACTCTTAACTTGCCTGAAGAGGAAGAGATCGTCGTGAAGCTTAAAAGTTCTCAGGTTATTGAGATTGAAGAGGCTGTGACGACGTTGAGAAAAATCACGAGAACTCGAGAGGACAGTAGAGTTCATCTCTGTTCTCCTATGATTCTCTCTGCTTTGCGATCTCTCATCGTCTCAAGGTACTCTGGCGTTCAAGTGAATTCCGTTGCGGCGCTTGTAAATCTATCTTTAGAGAATTTGAACAAGGTGAAGATCGTGCGGTCGGGGATTCTCCCTAATTTGATCGATGTTCTGAAAGGGGGTTCGCCGGAGGTTCAAGAACATGCTGCCGGCGCCATTTTCAGCTTAGCTCTCGAAGATAACAACAAGACGGCGATAGGCGTATTGGGTGCTCTACCACCATTGATAAGGCTGTTGTTATCCAACTCGGAGCAAACTCGGCACGACTCAGCTCTTGCTCTTTACCATTTATCCCACGTTCAGAGCAACCGTTCGAAGTTAGTGAAACTTGGGTCGGTGCCGATACTTCTCGGGATGGTGAAATCCCGTCACATGGCTGGCCGTATTTTGCTGACTTTGTGTAATTTAGCAGCTTGTTTTGAGGGCCGGGCGGCGTTGTTAGACTCCGGCGCGGTGGAGTGTTTGGTGGGGATGTTGAGAGAAAACGAGTTGGACTCTGAATCAACTAGGGAGAGTTGTGTGGCGGTTCTGTTTGGACTGAGTTTCGGCGGATTGAGGTTCAAAGGTCTGGCAAAAACCGCCGGAGCAATGGATGTTTTTATGGCAGTGGAGAAAAATGGGAGTGAAAGATCAAAAGAGAAGGTGAAGAGGATGATGGAGTATATGAAAGCCAGAGATGAAGAAGCTGAGGATGTGAATTGGGAGGAATTGCTCGACTCAGGTTGTTTCGGTAGCCGAAGTCGGTGTCGACTTGGTGCCGGAATGGACAGATCCACCGCAAACTCATCAGAATTCTGA

Protein sequence

MEFQEGLMKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPPHFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIPNLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNHAATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEIVVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGALPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILLTLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLAKTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRLGAGMDRSTANSSEF*
Homology
BLAST of CSPI05G28380 vs. ExPASy Swiss-Prot
Match: Q9FL17 (U-box domain-containing protein 40 OS=Arabidopsis thaliana OX=3702 GN=PUB40 PE=2 SV=2)

HSP 1 Score: 526.9 bits (1356), Expect = 2.7e-148
Identity = 301/528 (57.01%), Postives = 379/528 (71.78%), Query Frame = 0

Query: 39  RRKWRIFNRSSSSPFPSKPKPPHFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVC 98
           + KWR     SSS   S    P   EIP E LCPI+GSLMADP+IVSSGH++E ACV  C
Sbjct: 32  KSKWRTSLSRSSSSSSSNNNSPTKTEIPAEFLCPISGSLMADPIIVSSGHSYERACVIAC 91

Query: 99  KDLGVKPTLLDGSKPDFSSVIPNLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAA 158
           K LG  PT      PDFS+VIPNLALKS I +WC+     PP+PL+ ++AEKL+   +  
Sbjct: 92  KTLGFTPT----PPPDFSTVIPNLALKSAIHSWCERRCFPPPKPLNSAAAEKLILALMEK 151

Query: 159 H------SKSDEELIQGVAETPVVRFNHAATEVARRSSHFHSSSDESVSAVVPTLPLPLA 218
                  S S++ELIQ + + P VR NHAATE+ RR ++F+SSSDES+++   T  L L 
Sbjct: 152 KPQRRKVSVSEKELIQAIRDKPSVRLNHAATELDRRPNYFNSSSDESIASSSRT--LQLT 211

Query: 219 IRPSCCSSSSSSDNEIIGTLNLPEEEEIVVKLKSSQVIEIEEAVTTLRKITRTREDSRVH 278
            +PSC SS SS + E +     PEEE ++ KLKS+++ EIEEA+ ++R+ITR  E SR+ 
Sbjct: 212 TKPSCFSSPSSGEIESLEPNLTPEEEALLTKLKSNRISEIEEALISIRRITRIDESSRIS 271

Query: 279 LCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLENLNKVKIVRSGILPNLIDVLKGGSP 338
           LC+  ++SAL+SLIVSRY+ VQVN  A LVNLSLE  NKVKIVRSGI+P LIDVLK GS 
Sbjct: 272 LCTTRVISALKSLIVSRYATVQVNVTAVLVNLSLEKSNKVKIVRSGIVPPLIDVLKCGSV 331

Query: 339 EVQEHAAGAIFSLALEDNNKTAIGVLGALPPLIRLLLSNSEQTRHDSALALYHLSHVQSN 398
           E QEH+AG IFSLALED NKTAIGVLG L PL+ L+   +E TRHDSALALYHLS VQSN
Sbjct: 332 EAQEHSAGVIFSLALEDENKTAIGVLGGLEPLLHLIRVGTELTRHDSALALYHLSLVQSN 391

Query: 399 RSKLVKLGSVPILLGMVKSRHMAGRILLTLCNLAACFEGRAALLDSGAVECLVGMLRENE 458
           R KLVKLG+V +LLGMV    M GR+LL LCN+A+C   R ALLDSG VEC+VG+LR + 
Sbjct: 392 RGKLVKLGAVQMLLGMVSLGQMIGRVLLILCNMASCPVSRPALLDSGGVECMVGVLRRDR 451

Query: 459 LDSESTRESCVAVLFGLSF-GGLRFKGLAKTAGAMDVFMAVEKNGSERSKEKVKRMMEYM 518
             +ESTRESCVAVL+GLS  GGLRFKGLA  A A++  + VE++G ER+K+K +R++E +
Sbjct: 452 EVNESTRESCVAVLYGLSHDGGLRFKGLAMAANAVEELVKVERSGRERAKQKARRVLEVL 511

Query: 519 KARDE-----EAEDVNWEELLDSGCFGSRSRCRLGAGMDRSTANSSEF 555
           +A+ E     E E+++WEELL+SG   SRSRCRLG   ++S  NS+EF
Sbjct: 512 RAKIEDDDLVENEEIDWEELLNSGDV-SRSRCRLGG--EKSCVNSAEF 550

BLAST of CSPI05G28380 vs. ExPASy Swiss-Prot
Match: Q9FJP6 (U-box domain-containing protein 38 OS=Arabidopsis thaliana OX=3702 GN=PUB38 PE=1 SV=1)

HSP 1 Score: 436.8 bits (1122), Expect = 3.7e-121
Identity = 272/553 (49.19%), Postives = 362/553 (65.46%), Query Frame = 0

Query: 39  RRKWRIF-NRSSSSPFPSKPKPPHFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQV 98
           R +W  F +RSSSS   S  +    ++ P E LCPI+ S+M+DPV+VSSG TFE  CVQV
Sbjct: 6   RLRWNPFSHRSSSSTSSSSRQQQQEQQPPVEFLCPISKSVMSDPVVVSSGQTFERVCVQV 65

Query: 99  CKDLGVKPTLLDG--SKPDFSSVIPNLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKF 158
           C+DL   P L D   S PDFS++IPNL +KSTI  WC       PQP D+S+ E+++R+ 
Sbjct: 66  CRDLNFIPKLNDDEESLPDFSNIIPNLNMKSTIDTWCDTVGVSRPQPPDYSTVERILRQQ 125

Query: 159 VAAHS----KSDEELIQGVAETPVVRFNHAATEVARRSSHFHS--SSDESV-SAVVPTLP 218
           +         S++EL++ VA    +  +HA +E+  R    +S  SSDESV  A  P  P
Sbjct: 126 MPPPDVEIRVSEQELLRAVAHRAPMIIHHADSELMGRRDFNNSTTSSDESVIVAHSPFTP 185

Query: 219 LPLAIRPSCCSSSSSSDNEIIGTL-----------NLPEEEEIVV-KLKSSQVIEIEEAV 278
           LPL  RP+C S S SS +  I TL              EE+E++  KLKSS++ + E+ +
Sbjct: 186 LPLTTRPACFSPSPSSSSSEIETLTHHTFFSNSTSTATEEDEVIYNKLKSSEIFDQEQGL 245

Query: 279 TTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLENLNKVKIVR 338
             +RK+TRT +++RV LCSP ILS L+++IVSRYS VQ N++A+LVNLSL+  NK+ IVR
Sbjct: 246 IMMRKMTRTNDEARVSLCSPRILSLLKNMIVSRYSLVQTNALASLVNLSLDKKNKLTIVR 305

Query: 339 SGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGALPPLIRLL-LSNSEQT 398
            G +P LIDVLK GS E QEHAAG IFSL+LED+NK  IGVLGAL PL+  L  + S++T
Sbjct: 306 LGFVPILIDVLKSGSREAQEHAAGTIFSLSLEDDNKMPIGVLGALQPLLHALRAAESDRT 365

Query: 399 RHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILLTLCNLAACFEGRAAL 458
           RHDSALALYHL+  Q+NRSKLV+LG+VP L  MV+S   A R LL +CNLA C EGR+A+
Sbjct: 366 RHDSALALYHLTLNQTNRSKLVRLGAVPALFSMVRSGESASRALLVICNLACCSEGRSAM 425

Query: 459 LDSGAVECLVGMLRENELD-------SESTRESCVAVLFGLSFGGLRFKGLAKTAGAMDV 518
           LD+ AV  LVG LRE   +       S S RE+CVA LF LS   LRFKGLAK A A++V
Sbjct: 426 LDANAVAILVGKLREEWTEEPTEARSSSSARENCVAALFALSHESLRFKGLAKEARAVEV 485

Query: 519 FMAVEKNGSERSKEKVKRMMEYMKAR--DEEAED----VNWEELLDSGCFGS-RSRCRLG 555
              VE+ G+ER++EK K++++ M+ R  +++ ED    ++W+ ++DS   GS RSR R+G
Sbjct: 486 LKEVEERGTERAREKAKKILQLMRERVPEDDEEDGEGSIDWDRVIDSN--GSIRSRFRVG 545

BLAST of CSPI05G28380 vs. ExPASy Swiss-Prot
Match: Q0WUF6 (U-box domain-containing protein 41 OS=Arabidopsis thaliana OX=3702 GN=PUB41 PE=2 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 4.2e-109
Identity = 255/559 (45.62%), Postives = 337/559 (60.29%), Query Frame = 0

Query: 39  RRKWRIFN-RSSSSPFPSKPKPPHFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQV 98
           +++W  F+ RSSS+   + P+  H  E P E LCPITG LM+DPV+VSSG TFE   VQV
Sbjct: 5   KQRWFSFHQRSSSATTTTLPQHKH-DETPPEFLCPITGFLMSDPVVVSSGQTFERLSVQV 64

Query: 99  CKDLGVKPTLLDGSKPDFSSVIPNLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFV- 158
           C++LG  P LLDG++PD S+VIPNLA+KSTIF+WC     + P+P D +  E +VR  + 
Sbjct: 65  CRNLGYIPDLLDGTRPDLSTVIPNLAMKSTIFSWCDRQKVDHPRPPDAAYVEGVVRARMD 124

Query: 159 ------------AAHSKSDEELIQGVAETPVVRFNHAATEVARRSSHFHS--SSDESVSA 218
                             + E++  V E     ++     +  RS +  S  +S ESV+ 
Sbjct: 125 KDPNPSPGQSPGPGDKDPEPEILPPVEENSPSDYDAVMEAIRARSKNSMSPTTSLESVTI 184

Query: 219 VVPTLPLPLAIRPSCCSSSSSSDNEIIGTLN------------------LPEEEEIVVKL 278
              +     A+     S++SSS     G  +                   PEEEEI  KL
Sbjct: 185 GQSSYHPVRAVSMFSSSTTSSSSGVFAGADSPFRNAMSFSSTDHSSSPMSPEEEEIFNKL 244

Query: 279 KSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNL 338
           + + + + E+ +  LRK+TR+ ED RV LC+  ILS LRSL+VSRY+ VQ N+ A++VNL
Sbjct: 245 RGTDIFDHEQGLILLRKMTRSSEDLRVSLCTDRILSFLRSLLVSRYNLVQTNAAASVVNL 304

Query: 339 SLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGALPPL 398
           SLE  NKVKIVRSG +P LIDVLK G+ E QEH AGA+FSLALED NK  IGVLGA+ PL
Sbjct: 305 SLEKQNKVKIVRSGFVPLLIDVLKSGTTEAQEHVAGALFSLALEDENKMVIGVLGAVEPL 364

Query: 399 IRLLLSN-SEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILLTLC 458
           +  L S+ SE+ R D+ALALYHLS + SNR++LV+ G+VP LL MV+S     RILL LC
Sbjct: 365 LHALRSSESERARQDAALALYHLSLIPSNRTRLVRAGAVPTLLSMVRSGDSTSRILLVLC 424

Query: 459 NLAACFEGRAALLDSGAVECLVGMLRE-NELDSESTRESCVAVLFGLSFGGLRFKGLAKT 518
           NLAAC +G+ A+LD  AV  LVG LRE    DSE+ RE+CVAVL  L  G LRF+GLA  
Sbjct: 425 NLAACPDGKGAMLDGNAVAILVGKLREVGGGDSEAARENCVAVLLTLCQGNLRFRGLASE 484

Query: 519 AGAMDVFMAVEKNGSERSKEKVKRMMEYMKAR-------DEEAEDVNWEELLDSGCFGSR 555
           AGA +V M VE+NG+ER KEK  +++  M+          E AE   W  +L++      
Sbjct: 485 AGAEEVLMEVEENGNERVKEKASKILLAMRGGGGGESEFGENAEAREWNRMLEA---TGL 544

BLAST of CSPI05G28380 vs. ExPASy Swiss-Prot
Match: Q9STT1 (U-box domain-containing protein 39 OS=Arabidopsis thaliana OX=3702 GN=PUB39 PE=1 SV=2)

HSP 1 Score: 388.3 bits (996), Expect = 1.5e-106
Identity = 245/537 (45.62%), Postives = 334/537 (62.20%), Query Frame = 0

Query: 34  ISSTPRRKWRIFNRSSSSPFPSKPKPPHFKEIPKELLCPITGSLMADPVIVSSGHTFEAA 93
           +  T R KW  F+   S+   + P+     E P E LCPITG LM+DPV+V+SG TFE  
Sbjct: 1   MGDTGRHKWFSFHHHRSASATTMPQHNSPGETPTEFLCPITGFLMSDPVVVASGQTFERI 60

Query: 94  CVQVCKDLGVKPTLLDGSKPDFSSVIPNLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVR 153
            VQVC++L   P L DG++PD S+VIPNLA+KSTI +WC  +  E P+P D++  E +VR
Sbjct: 61  SVQVCRNLSFAPKLHDGTQPDLSTVIPNLAMKSTILSWCDRNKMEHPRPPDYAYVEGVVR 120

Query: 154 KFV------AAHSKSDEELIQGVAETPVVRFNHAATEVARRSSHFHSSSDESVSAVVPTL 213
             +      + H  +  E++  VAE      ++ +   A RS    S S  +   +  T 
Sbjct: 121 TRMDSLPPGSGHRIAKSEILPPVAENSNSNSDYDSVMGAIRSRSRTSLSSTTSLPLHQTR 180

Query: 214 PLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEIVVKLKSSQVIEIEEAVTTLRKITRTRE 273
           P+  + R    SS S+SD      ++ PEEEEI  KL S   I+ E+ +  LRK TR+ E
Sbjct: 181 PINHSTRIQ--SSFSTSDYSSFPPMS-PEEEEIYNKLTSVDTIDHEQGLIQLRKTTRSNE 240

Query: 274 DSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLENLNKVKIVRSGILPNLIDVL 333
            +R+ LC+  ILS LRSLIVSRY+ VQ N+ A++VNLSLE  NK+KIVRSG +P LIDVL
Sbjct: 241 TTRISLCTDRILSLLRSLIVSRYNIVQTNAAASIVNLSLEKPNKLKIVRSGFVPLLIDVL 300

Query: 334 KGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGALPPLIRLLLSN-SEQTRHDSALALYHL 393
           K GS E QEH  GA+FSLA+E+ NK  IGVLGA+ PL+  L S+ SE+ R D+ALALYHL
Sbjct: 301 KSGSTEAQEHVIGALFSLAVEEENKMVIGVLGAVEPLLHALRSSESERARQDAALALYHL 360

Query: 394 SHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILLTLCNLAACFEGRAALLDSGAVECLVG 453
           S + +NRS+LVK G+VP++L M++S   A RILL LCNLAAC EG+ A+LD  AV  LVG
Sbjct: 361 SLIPNNRSRLVKAGAVPMMLSMIRSGESASRILLLLCNLAACSEGKGAMLDGNAVSILVG 420

Query: 454 MLREN---ELDSESTRESCVAVLFGLSFGGLRFKGLAKTAGAMDVFMAV--EKNGSERSK 513
            LRE+   E D+ + RE+CV  L  LS G +RF+GLA  AGA ++   +   ++GS R K
Sbjct: 421 KLRESGGAESDA-AARENCVGALLTLSVGNMRFRGLASEAGAEEILTEIVESESGSGRLK 480

Query: 514 EKVKRMMEYMKARDEE----AEDVNWEELLDSGCFGSRSRCRLGAGMDRSTANSSEF 555
           EK  ++++ ++    E    AE   W  +L++    SRS+ + G G     A SS+F
Sbjct: 481 EKASKILQTLRGGGSEFGEGAEAREWNRMLEASGL-SRSQFQQG-GQKGGFAYSSQF 531

BLAST of CSPI05G28380 vs. ExPASy Swiss-Prot
Match: Q5XEZ8 (U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 2.1e-39
Identity = 140/474 (29.54%), Postives = 238/474 (50.21%), Query Frame = 0

Query: 65  IPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIPNLAL 124
           +P +  C ++  LM DPVIV+SG TFE   +Q   D+G+            +++ PN  +
Sbjct: 240 VPSDFRCSLSLELMTDPVIVASGQTFERVFIQKWIDMGLMVCPKTRQALSHTTLTPNFIV 299

Query: 125 KSTIFNWCKNSSSEPPQPLDFSSAEK----LVRKFVAA-----HSKS--DEELIQ----- 184
           ++ + +WC+ ++  PP PL+   + +    LV    A+     HS+S   EEL Q     
Sbjct: 300 RAFLASWCETNNVYPPDPLELIHSSEPFPLLVESVRASSSENGHSESLDAEELRQVFSRS 359

Query: 185 ----GVAETPVV---RFNHAATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSS 244
               G+    V    R N+AA + +   S+      E      P + +P  +R +   SS
Sbjct: 360 ASAPGIVSEVVCKTKRNNNAAADRSLTRSNTPWKFPEERHWRHPGI-IPATVRET--GSS 419

Query: 245 SSSDNEIIGTLNLPEEEEIVVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSA 304
           SS + E+         ++++  LKSS +    EA   +R + R   D+R+ +     + +
Sbjct: 420 SSIETEV---------KKLIDDLKSSSLDTQREATARIRILARNSTDNRIVIARCEAIPS 479

Query: 305 LRSLIVSRYSGVQVNSVAALVNLSLENLNKVKIVRSGILPNLIDVLKGG-SPEVQEHAAG 364
           L SL+ S    +Q ++V  L+NLS+ + NK  I  SG +  LI VLK G   E + ++A 
Sbjct: 480 LVSLLYSTDERIQADAVTCLLNLSINDNNKSLIAESGAIVPLIHVLKTGYLEEAKANSAA 539

Query: 365 AIFSLALEDNNKTAIGVLGALPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLG 424
            +FSL++ +  KT IG  GA+ PL+ LL S S   + D+A AL++LS    N++K+++ G
Sbjct: 540 TLFSLSVIEEYKTEIGEAGAIEPLVDLLGSGSLSGKKDAATALFNLSIHHENKTKVIEAG 599

Query: 425 SVPILLGMVKSRH-MAGRILLTLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTR 484
           +V  L+ ++     M  + ++ L NLA   EG+ A+ + G +  LV ++   EL S   +
Sbjct: 600 AVRYLVELMDPAFGMVEKAVVVLANLATVREGKIAIGEEGGIPVLVEVV---ELGSARGK 659

Query: 485 ESCVAVLFGLSFGGLRFKGLAKTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKA 514
           E+  A L  L     +F       G +   +A+ K+G+ R KEK + +++Y KA
Sbjct: 660 ENATAALLQLCTHSPKFCNNVIREGVIPPLVALTKSGTARGKEKAQNLLKYFKA 698

BLAST of CSPI05G28380 vs. ExPASy TrEMBL
Match: A0A0A0KX66 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G631520 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 2.7e-308
Identity = 553/554 (99.82%), Postives = 553/554 (99.82%), Query Frame = 0

Query: 1   MEFQEGLMKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP 60
           MEFQEGL KFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP
Sbjct: 1   MEFQEGLKKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP 60

Query: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP 120
           HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP
Sbjct: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP 120

Query: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH 180
           NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH
Sbjct: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH 180

Query: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240
           AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI
Sbjct: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240

Query: 241 VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300
           VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA
Sbjct: 241 VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300

Query: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA 360
           LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA
Sbjct: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA 360

Query: 361 LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL 420
           LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL
Sbjct: 361 LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL 420

Query: 421 TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480
           TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA
Sbjct: 421 TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480

Query: 481 KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL 540
           KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL
Sbjct: 481 KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL 540

Query: 541 GAGMDRSTANSSEF 555
           GAGMDRSTANSSEF
Sbjct: 541 GAGMDRSTANSSEF 554

BLAST of CSPI05G28380 vs. ExPASy TrEMBL
Match: A0A5A7T1Z7 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold808G00390 PE=4 SV=1)

HSP 1 Score: 1040.4 bits (2689), Expect = 2.7e-300
Identity = 539/554 (97.29%), Postives = 548/554 (98.92%), Query Frame = 0

Query: 1   MEFQEGLMKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP 60
           MEFQEGL+KFMFH DVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPK  
Sbjct: 1   MEFQEGLVKFMFHGDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPK-A 60

Query: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP 120
           HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLG+KPTLLDGSKPDFSSVIP
Sbjct: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGLKPTLLDGSKPDFSSVIP 120

Query: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH 180
           NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKF+AAHSKSDEELIQGVAETPVVRFNH
Sbjct: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFIAAHSKSDEELIQGVAETPVVRFNH 180

Query: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240
           AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI
Sbjct: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240

Query: 241 VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300
           VVKLKSSQVIE+EEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA
Sbjct: 241 VVKLKSSQVIEVEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300

Query: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA 360
           LVNLSLEN NKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLAL+DNNKTAIGVLGA
Sbjct: 301 LVNLSLENSNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALDDNNKTAIGVLGA 360

Query: 361 LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL 420
           LPPLIRLLLS+SEQTRHDSALALYHLSHVQSNRSKLVK GSVPILLGMVKSRHMAGRILL
Sbjct: 361 LPPLIRLLLSDSEQTRHDSALALYHLSHVQSNRSKLVKFGSVPILLGMVKSRHMAGRILL 420

Query: 421 TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480
            LCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA
Sbjct: 421 ILCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480

Query: 481 KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL 540
           KTAGAMD+FMA+EKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSR+RCRL
Sbjct: 481 KTAGAMDLFMAMEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRTRCRL 540

Query: 541 GAGMDRSTANSSEF 555
           GAGMDRSTANSSEF
Sbjct: 541 GAGMDRSTANSSEF 553

BLAST of CSPI05G28380 vs. ExPASy TrEMBL
Match: A0A1S3C8I3 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498047 PE=4 SV=1)

HSP 1 Score: 1001.5 bits (2588), Expect = 1.4e-288
Identity = 522/541 (96.49%), Postives = 531/541 (98.15%), Query Frame = 0

Query: 14  EDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPPHFKEIPKELLCPI 73
           E + P   FGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPK  HFKEIPKELLCPI
Sbjct: 2   EMLNPTGFFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPK-AHFKEIPKELLCPI 61

Query: 74  TGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIPNLALKSTIFNWCK 133
           TGSLMADPVIVSSGHTFEAACVQVCKDLG+KPTLLDGSKPDFSSVIPNLALKSTIFNWCK
Sbjct: 62  TGSLMADPVIVSSGHTFEAACVQVCKDLGLKPTLLDGSKPDFSSVIPNLALKSTIFNWCK 121

Query: 134 NSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNHAATEVARRSSHFH 193
           NSSSEPPQPLDFSSAEKLVRKF+AAHSKSDEELIQGVAETPVVRFNHAATEVARRSSHFH
Sbjct: 122 NSSSEPPQPLDFSSAEKLVRKFIAAHSKSDEELIQGVAETPVVRFNHAATEVARRSSHFH 181

Query: 194 SSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEIVVKLKSSQVIEIE 253
           SSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEIVVKLKSSQVIE+E
Sbjct: 182 SSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEIVVKLKSSQVIEVE 241

Query: 254 EAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLENLNKVK 313
           EAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLEN NKVK
Sbjct: 242 EAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLENSNKVK 301

Query: 314 IVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGALPPLIRLLLSNSE 373
           IVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLAL+DNNKTAIGVLGALPPLIRLLLS+SE
Sbjct: 302 IVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALDDNNKTAIGVLGALPPLIRLLLSDSE 361

Query: 374 QTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILLTLCNLAACFEGRA 433
           QTRHDSALALYHLSHVQSNRSKLVK GSVPILLGMVKSRHMAGRILL LCNLAACFEGRA
Sbjct: 362 QTRHDSALALYHLSHVQSNRSKLVKFGSVPILLGMVKSRHMAGRILLILCNLAACFEGRA 421

Query: 434 ALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLAKTAGAMDVFMAVE 493
           ALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLAKTAGAMD+FMA+E
Sbjct: 422 ALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLAKTAGAMDLFMAME 481

Query: 494 KNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRLGAGMDRSTANSSE 553
           KNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSR+RCRLGAGMDRSTANSSE
Sbjct: 482 KNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRTRCRLGAGMDRSTANSSE 541

Query: 554 F 555
           F
Sbjct: 542 F 541

BLAST of CSPI05G28380 vs. ExPASy TrEMBL
Match: A0A6J1K2N7 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489531 PE=4 SV=1)

HSP 1 Score: 968.0 bits (2501), Expect = 1.7e-278
Identity = 499/554 (90.07%), Postives = 526/554 (94.95%), Query Frame = 0

Query: 1   MEFQEGLMKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP 60
           MEFQEGLMKFM H  +K NWV  K+SP VAVSEI+S+PRRKWRIFNRSSSSPFPS  KPP
Sbjct: 1   MEFQEGLMKFMIHRHIKHNWVLEKNSPPVAVSEITSSPRRKWRIFNRSSSSPFPS--KPP 60

Query: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP 120
             KEIPKELLCPI+GSLMADPVIVSSGHTFEAACVQVCKDLG+KPTLLDGS PDFSSVIP
Sbjct: 61  QSKEIPKELLCPISGSLMADPVIVSSGHTFEAACVQVCKDLGLKPTLLDGSNPDFSSVIP 120

Query: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH 180
           NLALKSTI NWCKNSSSE P+PLDFSSAEKLVR+F+AAH+KSDEELIQG+AE PVVRFNH
Sbjct: 121 NLALKSTILNWCKNSSSESPKPLDFSSAEKLVRQFMAAHTKSDEELIQGIAENPVVRFNH 180

Query: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240
           AATEV RR+SHFHSSSDESV A VPTLPLPLA RPSCCSSSSSSD E+I  LNLPEEEEI
Sbjct: 181 AATEVTRRTSHFHSSSDESVGAAVPTLPLPLATRPSCCSSSSSSDIEVISDLNLPEEEEI 240

Query: 241 VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300
           + KLKSSQVIEIEEAVT LRKITRTREDSRVHLCSP+ILSALRSLIVSRY+GVQVNSVAA
Sbjct: 241 IAKLKSSQVIEIEEAVTALRKITRTREDSRVHLCSPLILSALRSLIVSRYTGVQVNSVAA 300

Query: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA 360
           LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALED+NKTAIGVLGA
Sbjct: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDDNKTAIGVLGA 360

Query: 361 LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL 420
           LPPLIRLL+S+SEQTRHDSALALYHLSHVQSNRSKLVKLGSVP+ LGMVKS HMAGRILL
Sbjct: 361 LPPLIRLLVSDSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPVFLGMVKSGHMAGRILL 420

Query: 421 TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480
            LCNLAACFEGRAALLDSGAVECLVGM+RENELD+ESTRESCVAVLFGLS+GGLRFKGLA
Sbjct: 421 ILCNLAACFEGRAALLDSGAVECLVGMMRENELDTESTRESCVAVLFGLSYGGLRFKGLA 480

Query: 481 KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL 540
           KTAGAM+VFMAVEKNGSERSKEKVKR+MEYMKARDEEAEDVNWEELLDSGCFGSR+RCR+
Sbjct: 481 KTAGAMEVFMAVEKNGSERSKEKVKRIMEYMKARDEEAEDVNWEELLDSGCFGSRTRCRI 540

Query: 541 GAGMDRSTANSSEF 555
           GAGMD STANSSEF
Sbjct: 541 GAGMDISTANSSEF 552

BLAST of CSPI05G28380 vs. ExPASy TrEMBL
Match: A0A6J1FNQ0 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111445559 PE=4 SV=1)

HSP 1 Score: 967.6 bits (2500), Expect = 2.2e-278
Identity = 500/554 (90.25%), Postives = 525/554 (94.77%), Query Frame = 0

Query: 1   MEFQEGLMKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP 60
           MEFQEGLMKFM H  VK NWV  K+SP VAVSEI+S+PRRKWRIFNRSSSSPFPS  KPP
Sbjct: 1   MEFQEGLMKFMIHRHVKHNWVLEKNSPPVAVSEITSSPRRKWRIFNRSSSSPFPS--KPP 60

Query: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP 120
             KEIPKELLCPI+GSLMADPVIVSSGHTFEAACVQVCKDLG+KPTLLDGS PDFSSVIP
Sbjct: 61  QSKEIPKELLCPISGSLMADPVIVSSGHTFEAACVQVCKDLGLKPTLLDGSNPDFSSVIP 120

Query: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH 180
           NLALKSTI NWCKNSSSE P+PLDFSSAEKLVR+F+AAH+KSDEELIQG+AE PVVRFNH
Sbjct: 121 NLALKSTILNWCKNSSSESPKPLDFSSAEKLVRQFMAAHTKSDEELIQGIAENPVVRFNH 180

Query: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240
           AATEV RR+SHFHSSSDESV A V TLPLPLA RPSCCSSSSSSD E+I  LNLPEEEEI
Sbjct: 181 AATEVTRRTSHFHSSSDESVGAAVQTLPLPLATRPSCCSSSSSSDIEVISDLNLPEEEEI 240

Query: 241 VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300
           + KLKSSQVIEIEEAVT LRKITRTREDSRVHLCSP+ILS+LRSLIVSRY+GVQVNSVAA
Sbjct: 241 IAKLKSSQVIEIEEAVTALRKITRTREDSRVHLCSPLILSSLRSLIVSRYTGVQVNSVAA 300

Query: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA 360
           LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALED+NKTAIGVLGA
Sbjct: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDDNKTAIGVLGA 360

Query: 361 LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL 420
           LPPLIRLL+S SEQTRHDSALALYHLSHVQSNRSKLVKLGSVP+ LGMVKS HMAGRILL
Sbjct: 361 LPPLIRLLVSESEQTRHDSALALYHLSHVQSNRSKLVKLGSVPVFLGMVKSGHMAGRILL 420

Query: 421 TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480
            LCNLAACFEGRAALLDSGAVECLVGMLRENELD+ESTRESCVAVLFGLS+GGLRFKGLA
Sbjct: 421 ILCNLAACFEGRAALLDSGAVECLVGMLRENELDTESTRESCVAVLFGLSYGGLRFKGLA 480

Query: 481 KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL 540
           KTAGAM+VFMAVEKNGSERSKEKVKR+MEYMKARDEEAEDVNWEELLDSGCFGSR+RCR+
Sbjct: 481 KTAGAMEVFMAVEKNGSERSKEKVKRIMEYMKARDEEAEDVNWEELLDSGCFGSRTRCRI 540

Query: 541 GAGMDRSTANSSEF 555
           GAGMDRSTANSSEF
Sbjct: 541 GAGMDRSTANSSEF 552

BLAST of CSPI05G28380 vs. NCBI nr
Match: XP_011655976.1 (U-box domain-containing protein 40 [Cucumis sativus] >KGN52401.1 hypothetical protein Csa_009420 [Cucumis sativus])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.5e-308
Identity = 553/554 (99.82%), Postives = 553/554 (99.82%), Query Frame = 0

Query: 1   MEFQEGLMKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP 60
           MEFQEGL KFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP
Sbjct: 1   MEFQEGLKKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP 60

Query: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP 120
           HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP
Sbjct: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP 120

Query: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH 180
           NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH
Sbjct: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH 180

Query: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240
           AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI
Sbjct: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240

Query: 241 VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300
           VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA
Sbjct: 241 VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300

Query: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA 360
           LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA
Sbjct: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA 360

Query: 361 LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL 420
           LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL
Sbjct: 361 LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL 420

Query: 421 TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480
           TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA
Sbjct: 421 TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480

Query: 481 KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL 540
           KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL
Sbjct: 481 KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL 540

Query: 541 GAGMDRSTANSSEF 555
           GAGMDRSTANSSEF
Sbjct: 541 GAGMDRSTANSSEF 554

BLAST of CSPI05G28380 vs. NCBI nr
Match: KAA0037472.1 (U-box domain-containing protein 40 [Cucumis melo var. makuwa] >TYK01948.1 U-box domain-containing protein 40 [Cucumis melo var. makuwa])

HSP 1 Score: 1040.4 bits (2689), Expect = 5.5e-300
Identity = 539/554 (97.29%), Postives = 548/554 (98.92%), Query Frame = 0

Query: 1   MEFQEGLMKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP 60
           MEFQEGL+KFMFH DVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPK  
Sbjct: 1   MEFQEGLVKFMFHGDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPK-A 60

Query: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP 120
           HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLG+KPTLLDGSKPDFSSVIP
Sbjct: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGLKPTLLDGSKPDFSSVIP 120

Query: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH 180
           NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKF+AAHSKSDEELIQGVAETPVVRFNH
Sbjct: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFIAAHSKSDEELIQGVAETPVVRFNH 180

Query: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240
           AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI
Sbjct: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240

Query: 241 VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300
           VVKLKSSQVIE+EEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA
Sbjct: 241 VVKLKSSQVIEVEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300

Query: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA 360
           LVNLSLEN NKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLAL+DNNKTAIGVLGA
Sbjct: 301 LVNLSLENSNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALDDNNKTAIGVLGA 360

Query: 361 LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL 420
           LPPLIRLLLS+SEQTRHDSALALYHLSHVQSNRSKLVK GSVPILLGMVKSRHMAGRILL
Sbjct: 361 LPPLIRLLLSDSEQTRHDSALALYHLSHVQSNRSKLVKFGSVPILLGMVKSRHMAGRILL 420

Query: 421 TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480
            LCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA
Sbjct: 421 ILCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480

Query: 481 KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL 540
           KTAGAMD+FMA+EKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSR+RCRL
Sbjct: 481 KTAGAMDLFMAMEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRTRCRL 540

Query: 541 GAGMDRSTANSSEF 555
           GAGMDRSTANSSEF
Sbjct: 541 GAGMDRSTANSSEF 553

BLAST of CSPI05G28380 vs. NCBI nr
Match: XP_008458723.1 (PREDICTED: U-box domain-containing protein 40 [Cucumis melo])

HSP 1 Score: 1001.5 bits (2588), Expect = 2.8e-288
Identity = 522/541 (96.49%), Postives = 531/541 (98.15%), Query Frame = 0

Query: 14  EDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPPHFKEIPKELLCPI 73
           E + P   FGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPK  HFKEIPKELLCPI
Sbjct: 2   EMLNPTGFFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPK-AHFKEIPKELLCPI 61

Query: 74  TGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIPNLALKSTIFNWCK 133
           TGSLMADPVIVSSGHTFEAACVQVCKDLG+KPTLLDGSKPDFSSVIPNLALKSTIFNWCK
Sbjct: 62  TGSLMADPVIVSSGHTFEAACVQVCKDLGLKPTLLDGSKPDFSSVIPNLALKSTIFNWCK 121

Query: 134 NSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNHAATEVARRSSHFH 193
           NSSSEPPQPLDFSSAEKLVRKF+AAHSKSDEELIQGVAETPVVRFNHAATEVARRSSHFH
Sbjct: 122 NSSSEPPQPLDFSSAEKLVRKFIAAHSKSDEELIQGVAETPVVRFNHAATEVARRSSHFH 181

Query: 194 SSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEIVVKLKSSQVIEIE 253
           SSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEIVVKLKSSQVIE+E
Sbjct: 182 SSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEIVVKLKSSQVIEVE 241

Query: 254 EAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLENLNKVK 313
           EAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLEN NKVK
Sbjct: 242 EAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLENSNKVK 301

Query: 314 IVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGALPPLIRLLLSNSE 373
           IVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLAL+DNNKTAIGVLGALPPLIRLLLS+SE
Sbjct: 302 IVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALDDNNKTAIGVLGALPPLIRLLLSDSE 361

Query: 374 QTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILLTLCNLAACFEGRA 433
           QTRHDSALALYHLSHVQSNRSKLVK GSVPILLGMVKSRHMAGRILL LCNLAACFEGRA
Sbjct: 362 QTRHDSALALYHLSHVQSNRSKLVKFGSVPILLGMVKSRHMAGRILLILCNLAACFEGRA 421

Query: 434 ALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLAKTAGAMDVFMAVE 493
           ALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLAKTAGAMD+FMA+E
Sbjct: 422 ALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLAKTAGAMDLFMAME 481

Query: 494 KNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRLGAGMDRSTANSSE 553
           KNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSR+RCRLGAGMDRSTANSSE
Sbjct: 482 KNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRTRCRLGAGMDRSTANSSE 541

Query: 554 F 555
           F
Sbjct: 542 F 541

BLAST of CSPI05G28380 vs. NCBI nr
Match: XP_038889155.1 (U-box domain-containing protein 40 [Benincasa hispida])

HSP 1 Score: 986.1 bits (2548), Expect = 1.2e-283
Identity = 506/554 (91.34%), Postives = 533/554 (96.21%), Query Frame = 0

Query: 1   MEFQEGLMKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP 60
           MEFQEGLMKFM H  +KPNWV  KDSPSVAVSEI+++PRRKWRIF+RSSSSPFPSKP+  
Sbjct: 1   MEFQEGLMKFMVHRHIKPNWVLEKDSPSVAVSEITNSPRRKWRIFSRSSSSPFPSKPQ-- 60

Query: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP 120
             KEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLG+ PTLLDGSKPDFSSVIP
Sbjct: 61  -LKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGLNPTLLDGSKPDFSSVIP 120

Query: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH 180
           NLALKS IFNWCKNSSSEPP+PLDFSSAEKLVRKF+AAHSKSDEELIQG+AE PVVRFNH
Sbjct: 121 NLALKSAIFNWCKNSSSEPPKPLDFSSAEKLVRKFMAAHSKSDEELIQGIAENPVVRFNH 180

Query: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240
           AATEV RRSSHFHSSSDESVSA VPTLPLPL  RPSCCSSSSSSD EIIGTLNLPEEEEI
Sbjct: 181 AATEVTRRSSHFHSSSDESVSAAVPTLPLPLTTRPSCCSSSSSSDVEIIGTLNLPEEEEI 240

Query: 241 VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300
           + KLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSP++LSALRSLIVSRY+GVQVN+VAA
Sbjct: 241 IAKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPLVLSALRSLIVSRYTGVQVNAVAA 300

Query: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA 360
           LVNLSLEN+NKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALED+NKTAIGVLGA
Sbjct: 301 LVNLSLENVNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDDNKTAIGVLGA 360

Query: 361 LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL 420
           LPPLIRLL+S+SEQTRHDSALALYHLSHVQSNRSKLVKLGSVP+ LGMVKSRHMAG ILL
Sbjct: 361 LPPLIRLLVSDSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPVFLGMVKSRHMAGCILL 420

Query: 421 TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480
            LCN+AACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLS+GGLRFKGLA
Sbjct: 421 ILCNIAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSYGGLRFKGLA 480

Query: 481 KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL 540
           KTAGAMDVFMA+EKNG+ERSKEKVKR++EYMKARDEEAEDVNWEELLDSGCFGSR+RCRL
Sbjct: 481 KTAGAMDVFMAIEKNGTERSKEKVKRIIEYMKARDEEAEDVNWEELLDSGCFGSRTRCRL 540

Query: 541 GAGMDRSTANSSEF 555
           GAGMDRSTANSSEF
Sbjct: 541 GAGMDRSTANSSEF 551

BLAST of CSPI05G28380 vs. NCBI nr
Match: XP_022993578.1 (U-box domain-containing protein 40 [Cucurbita maxima])

HSP 1 Score: 968.0 bits (2501), Expect = 3.5e-278
Identity = 499/554 (90.07%), Postives = 526/554 (94.95%), Query Frame = 0

Query: 1   MEFQEGLMKFMFHEDVKPNWVFGKDSPSVAVSEISSTPRRKWRIFNRSSSSPFPSKPKPP 60
           MEFQEGLMKFM H  +K NWV  K+SP VAVSEI+S+PRRKWRIFNRSSSSPFPS  KPP
Sbjct: 1   MEFQEGLMKFMIHRHIKHNWVLEKNSPPVAVSEITSSPRRKWRIFNRSSSSPFPS--KPP 60

Query: 61  HFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIP 120
             KEIPKELLCPI+GSLMADPVIVSSGHTFEAACVQVCKDLG+KPTLLDGS PDFSSVIP
Sbjct: 61  QSKEIPKELLCPISGSLMADPVIVSSGHTFEAACVQVCKDLGLKPTLLDGSNPDFSSVIP 120

Query: 121 NLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAAHSKSDEELIQGVAETPVVRFNH 180
           NLALKSTI NWCKNSSSE P+PLDFSSAEKLVR+F+AAH+KSDEELIQG+AE PVVRFNH
Sbjct: 121 NLALKSTILNWCKNSSSESPKPLDFSSAEKLVRQFMAAHTKSDEELIQGIAENPVVRFNH 180

Query: 181 AATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEEEEI 240
           AATEV RR+SHFHSSSDESV A VPTLPLPLA RPSCCSSSSSSD E+I  LNLPEEEEI
Sbjct: 181 AATEVTRRTSHFHSSSDESVGAAVPTLPLPLATRPSCCSSSSSSDIEVISDLNLPEEEEI 240

Query: 241 VVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAA 300
           + KLKSSQVIEIEEAVT LRKITRTREDSRVHLCSP+ILSALRSLIVSRY+GVQVNSVAA
Sbjct: 241 IAKLKSSQVIEIEEAVTALRKITRTREDSRVHLCSPLILSALRSLIVSRYTGVQVNSVAA 300

Query: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGA 360
           LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALED+NKTAIGVLGA
Sbjct: 301 LVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDDNKTAIGVLGA 360

Query: 361 LPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILL 420
           LPPLIRLL+S+SEQTRHDSALALYHLSHVQSNRSKLVKLGSVP+ LGMVKS HMAGRILL
Sbjct: 361 LPPLIRLLVSDSEQTRHDSALALYHLSHVQSNRSKLVKLGSVPVFLGMVKSGHMAGRILL 420

Query: 421 TLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTRESCVAVLFGLSFGGLRFKGLA 480
            LCNLAACFEGRAALLDSGAVECLVGM+RENELD+ESTRESCVAVLFGLS+GGLRFKGLA
Sbjct: 421 ILCNLAACFEGRAALLDSGAVECLVGMMRENELDTESTRESCVAVLFGLSYGGLRFKGLA 480

Query: 481 KTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKARDEEAEDVNWEELLDSGCFGSRSRCRL 540
           KTAGAM+VFMAVEKNGSERSKEKVKR+MEYMKARDEEAEDVNWEELLDSGCFGSR+RCR+
Sbjct: 481 KTAGAMEVFMAVEKNGSERSKEKVKRIMEYMKARDEEAEDVNWEELLDSGCFGSRTRCRI 540

Query: 541 GAGMDRSTANSSEF 555
           GAGMD STANSSEF
Sbjct: 541 GAGMDISTANSSEF 552

BLAST of CSPI05G28380 vs. TAIR 10
Match: AT5G40140.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 526.9 bits (1356), Expect = 1.9e-149
Identity = 301/528 (57.01%), Postives = 379/528 (71.78%), Query Frame = 0

Query: 39  RRKWRIFNRSSSSPFPSKPKPPHFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQVC 98
           + KWR     SSS   S    P   EIP E LCPI+GSLMADP+IVSSGH++E ACV  C
Sbjct: 32  KSKWRTSLSRSSSSSSSNNNSPTKTEIPAEFLCPISGSLMADPIIVSSGHSYERACVIAC 91

Query: 99  KDLGVKPTLLDGSKPDFSSVIPNLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFVAA 158
           K LG  PT      PDFS+VIPNLALKS I +WC+     PP+PL+ ++AEKL+   +  
Sbjct: 92  KTLGFTPT----PPPDFSTVIPNLALKSAIHSWCERRCFPPPKPLNSAAAEKLILALMEK 151

Query: 159 H------SKSDEELIQGVAETPVVRFNHAATEVARRSSHFHSSSDESVSAVVPTLPLPLA 218
                  S S++ELIQ + + P VR NHAATE+ RR ++F+SSSDES+++   T  L L 
Sbjct: 152 KPQRRKVSVSEKELIQAIRDKPSVRLNHAATELDRRPNYFNSSSDESIASSSRT--LQLT 211

Query: 219 IRPSCCSSSSSSDNEIIGTLNLPEEEEIVVKLKSSQVIEIEEAVTTLRKITRTREDSRVH 278
            +PSC SS SS + E +     PEEE ++ KLKS+++ EIEEA+ ++R+ITR  E SR+ 
Sbjct: 212 TKPSCFSSPSSGEIESLEPNLTPEEEALLTKLKSNRISEIEEALISIRRITRIDESSRIS 271

Query: 279 LCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLENLNKVKIVRSGILPNLIDVLKGGSP 338
           LC+  ++SAL+SLIVSRY+ VQVN  A LVNLSLE  NKVKIVRSGI+P LIDVLK GS 
Sbjct: 272 LCTTRVISALKSLIVSRYATVQVNVTAVLVNLSLEKSNKVKIVRSGIVPPLIDVLKCGSV 331

Query: 339 EVQEHAAGAIFSLALEDNNKTAIGVLGALPPLIRLLLSNSEQTRHDSALALYHLSHVQSN 398
           E QEH+AG IFSLALED NKTAIGVLG L PL+ L+   +E TRHDSALALYHLS VQSN
Sbjct: 332 EAQEHSAGVIFSLALEDENKTAIGVLGGLEPLLHLIRVGTELTRHDSALALYHLSLVQSN 391

Query: 399 RSKLVKLGSVPILLGMVKSRHMAGRILLTLCNLAACFEGRAALLDSGAVECLVGMLRENE 458
           R KLVKLG+V +LLGMV    M GR+LL LCN+A+C   R ALLDSG VEC+VG+LR + 
Sbjct: 392 RGKLVKLGAVQMLLGMVSLGQMIGRVLLILCNMASCPVSRPALLDSGGVECMVGVLRRDR 451

Query: 459 LDSESTRESCVAVLFGLSF-GGLRFKGLAKTAGAMDVFMAVEKNGSERSKEKVKRMMEYM 518
             +ESTRESCVAVL+GLS  GGLRFKGLA  A A++  + VE++G ER+K+K +R++E +
Sbjct: 452 EVNESTRESCVAVLYGLSHDGGLRFKGLAMAANAVEELVKVERSGRERAKQKARRVLEVL 511

Query: 519 KARDE-----EAEDVNWEELLDSGCFGSRSRCRLGAGMDRSTANSSEF 555
           +A+ E     E E+++WEELL+SG   SRSRCRLG   ++S  NS+EF
Sbjct: 512 RAKIEDDDLVENEEIDWEELLNSGDV-SRSRCRLGG--EKSCVNSAEF 550

BLAST of CSPI05G28380 vs. TAIR 10
Match: AT5G65200.1 (plant U-box 38 )

HSP 1 Score: 436.8 bits (1122), Expect = 2.6e-122
Identity = 272/553 (49.19%), Postives = 362/553 (65.46%), Query Frame = 0

Query: 39  RRKWRIF-NRSSSSPFPSKPKPPHFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQV 98
           R +W  F +RSSSS   S  +    ++ P E LCPI+ S+M+DPV+VSSG TFE  CVQV
Sbjct: 6   RLRWNPFSHRSSSSTSSSSRQQQQEQQPPVEFLCPISKSVMSDPVVVSSGQTFERVCVQV 65

Query: 99  CKDLGVKPTLLDG--SKPDFSSVIPNLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKF 158
           C+DL   P L D   S PDFS++IPNL +KSTI  WC       PQP D+S+ E+++R+ 
Sbjct: 66  CRDLNFIPKLNDDEESLPDFSNIIPNLNMKSTIDTWCDTVGVSRPQPPDYSTVERILRQQ 125

Query: 159 VAAHS----KSDEELIQGVAETPVVRFNHAATEVARRSSHFHS--SSDESV-SAVVPTLP 218
           +         S++EL++ VA    +  +HA +E+  R    +S  SSDESV  A  P  P
Sbjct: 126 MPPPDVEIRVSEQELLRAVAHRAPMIIHHADSELMGRRDFNNSTTSSDESVIVAHSPFTP 185

Query: 219 LPLAIRPSCCSSSSSSDNEIIGTL-----------NLPEEEEIVV-KLKSSQVIEIEEAV 278
           LPL  RP+C S S SS +  I TL              EE+E++  KLKSS++ + E+ +
Sbjct: 186 LPLTTRPACFSPSPSSSSSEIETLTHHTFFSNSTSTATEEDEVIYNKLKSSEIFDQEQGL 245

Query: 279 TTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNLSLENLNKVKIVR 338
             +RK+TRT +++RV LCSP ILS L+++IVSRYS VQ N++A+LVNLSL+  NK+ IVR
Sbjct: 246 IMMRKMTRTNDEARVSLCSPRILSLLKNMIVSRYSLVQTNALASLVNLSLDKKNKLTIVR 305

Query: 339 SGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGALPPLIRLL-LSNSEQT 398
            G +P LIDVLK GS E QEHAAG IFSL+LED+NK  IGVLGAL PL+  L  + S++T
Sbjct: 306 LGFVPILIDVLKSGSREAQEHAAGTIFSLSLEDDNKMPIGVLGALQPLLHALRAAESDRT 365

Query: 399 RHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILLTLCNLAACFEGRAAL 458
           RHDSALALYHL+  Q+NRSKLV+LG+VP L  MV+S   A R LL +CNLA C EGR+A+
Sbjct: 366 RHDSALALYHLTLNQTNRSKLVRLGAVPALFSMVRSGESASRALLVICNLACCSEGRSAM 425

Query: 459 LDSGAVECLVGMLRENELD-------SESTRESCVAVLFGLSFGGLRFKGLAKTAGAMDV 518
           LD+ AV  LVG LRE   +       S S RE+CVA LF LS   LRFKGLAK A A++V
Sbjct: 426 LDANAVAILVGKLREEWTEEPTEARSSSSARENCVAALFALSHESLRFKGLAKEARAVEV 485

Query: 519 FMAVEKNGSERSKEKVKRMMEYMKAR--DEEAED----VNWEELLDSGCFGS-RSRCRLG 555
              VE+ G+ER++EK K++++ M+ R  +++ ED    ++W+ ++DS   GS RSR R+G
Sbjct: 486 LKEVEERGTERAREKAKKILQLMRERVPEDDEEDGEGSIDWDRVIDSN--GSIRSRFRVG 545

BLAST of CSPI05G28380 vs. TAIR 10
Match: AT5G62560.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 396.7 bits (1018), Expect = 3.0e-110
Identity = 255/559 (45.62%), Postives = 337/559 (60.29%), Query Frame = 0

Query: 39  RRKWRIFN-RSSSSPFPSKPKPPHFKEIPKELLCPITGSLMADPVIVSSGHTFEAACVQV 98
           +++W  F+ RSSS+   + P+  H  E P E LCPITG LM+DPV+VSSG TFE   VQV
Sbjct: 5   KQRWFSFHQRSSSATTTTLPQHKH-DETPPEFLCPITGFLMSDPVVVSSGQTFERLSVQV 64

Query: 99  CKDLGVKPTLLDGSKPDFSSVIPNLALKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFV- 158
           C++LG  P LLDG++PD S+VIPNLA+KSTIF+WC     + P+P D +  E +VR  + 
Sbjct: 65  CRNLGYIPDLLDGTRPDLSTVIPNLAMKSTIFSWCDRQKVDHPRPPDAAYVEGVVRARMD 124

Query: 159 ------------AAHSKSDEELIQGVAETPVVRFNHAATEVARRSSHFHS--SSDESVSA 218
                             + E++  V E     ++     +  RS +  S  +S ESV+ 
Sbjct: 125 KDPNPSPGQSPGPGDKDPEPEILPPVEENSPSDYDAVMEAIRARSKNSMSPTTSLESVTI 184

Query: 219 VVPTLPLPLAIRPSCCSSSSSSDNEIIGTLN------------------LPEEEEIVVKL 278
              +     A+     S++SSS     G  +                   PEEEEI  KL
Sbjct: 185 GQSSYHPVRAVSMFSSSTTSSSSGVFAGADSPFRNAMSFSSTDHSSSPMSPEEEEIFNKL 244

Query: 279 KSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNSVAALVNL 338
           + + + + E+ +  LRK+TR+ ED RV LC+  ILS LRSL+VSRY+ VQ N+ A++VNL
Sbjct: 245 RGTDIFDHEQGLILLRKMTRSSEDLRVSLCTDRILSFLRSLLVSRYNLVQTNAAASVVNL 304

Query: 339 SLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGVLGALPPL 398
           SLE  NKVKIVRSG +P LIDVLK G+ E QEH AGA+FSLALED NK  IGVLGA+ PL
Sbjct: 305 SLEKQNKVKIVRSGFVPLLIDVLKSGTTEAQEHVAGALFSLALEDENKMVIGVLGAVEPL 364

Query: 399 IRLLLSN-SEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAGRILLTLC 458
           +  L S+ SE+ R D+ALALYHLS + SNR++LV+ G+VP LL MV+S     RILL LC
Sbjct: 365 LHALRSSESERARQDAALALYHLSLIPSNRTRLVRAGAVPTLLSMVRSGDSTSRILLVLC 424

Query: 459 NLAACFEGRAALLDSGAVECLVGMLRE-NELDSESTRESCVAVLFGLSFGGLRFKGLAKT 518
           NLAAC +G+ A+LD  AV  LVG LRE    DSE+ RE+CVAVL  L  G LRF+GLA  
Sbjct: 425 NLAACPDGKGAMLDGNAVAILVGKLREVGGGDSEAARENCVAVLLTLCQGNLRFRGLASE 484

Query: 519 AGAMDVFMAVEKNGSERSKEKVKRMMEYMKAR-------DEEAEDVNWEELLDSGCFGSR 555
           AGA +V M VE+NG+ER KEK  +++  M+          E AE   W  +L++      
Sbjct: 485 AGAEEVLMEVEENGNERVKEKASKILLAMRGGGGGESEFGENAEAREWNRMLEA---TGL 544

BLAST of CSPI05G28380 vs. TAIR 10
Match: AT3G47820.1 (PLANT U-BOX 39 )

HSP 1 Score: 381.7 bits (979), Expect = 9.9e-106
Identity = 238/507 (46.94%), Postives = 322/507 (63.51%), Query Frame = 0

Query: 64  EIPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIPNLA 123
           E P E LCPITG LM+DPV+V+SG TFE   VQVC++L   P L DG++PD S+VIPNLA
Sbjct: 9   ETPTEFLCPITGFLMSDPVVVASGQTFERISVQVCRNLSFAPKLHDGTQPDLSTVIPNLA 68

Query: 124 LKSTIFNWCKNSSSEPPQPLDFSSAEKLVRKFV------AAHSKSDEELIQGVAETPVVR 183
           +KSTI +WC  +  E P+P D++  E +VR  +      + H  +  E++  VAE     
Sbjct: 69  MKSTILSWCDRNKMEHPRPPDYAYVEGVVRTRMDSLPPGSGHRIAKSEILPPVAENSNSN 128

Query: 184 FNHAATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSSSSSDNEIIGTLNLPEE 243
            ++ +   A RS    S S  +   +  T P+  + R    SS S+SD      ++ PEE
Sbjct: 129 SDYDSVMGAIRSRSRTSLSSTTSLPLHQTRPINHSTRIQ--SSFSTSDYSSFPPMS-PEE 188

Query: 244 EEIVVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSALRSLIVSRYSGVQVNS 303
           EEI  KL S   I+ E+ +  LRK TR+ E +R+ LC+  ILS LRSLIVSRY+ VQ N+
Sbjct: 189 EEIYNKLTSVDTIDHEQGLIQLRKTTRSNETTRISLCTDRILSLLRSLIVSRYNIVQTNA 248

Query: 304 VAALVNLSLENLNKVKIVRSGILPNLIDVLKGGSPEVQEHAAGAIFSLALEDNNKTAIGV 363
            A++VNLSLE  NK+KIVRSG +P LIDVLK GS E QEH  GA+FSLA+E+ NK  IGV
Sbjct: 249 AASIVNLSLEKPNKLKIVRSGFVPLLIDVLKSGSTEAQEHVIGALFSLAVEEENKMVIGV 308

Query: 364 LGALPPLIRLLLSN-SEQTRHDSALALYHLSHVQSNRSKLVKLGSVPILLGMVKSRHMAG 423
           LGA+ PL+  L S+ SE+ R D+ALALYHLS + +NRS+LVK G+VP++L M++S   A 
Sbjct: 309 LGAVEPLLHALRSSESERARQDAALALYHLSLIPNNRSRLVKAGAVPMMLSMIRSGESAS 368

Query: 424 RILLTLCNLAACFEGRAALLDSGAVECLVGMLREN---ELDSESTRESCVAVLFGLSFGG 483
           RILL LCNLAAC EG+ A+LD  AV  LVG LRE+   E D+ + RE+CV  L  LS G 
Sbjct: 369 RILLLLCNLAACSEGKGAMLDGNAVSILVGKLRESGGAESDA-AARENCVGALLTLSVGN 428

Query: 484 LRFKGLAKTAGAMDVFMAV--EKNGSERSKEKVKRMMEYMKARDEE----AEDVNWEELL 543
           +RF+GLA  AGA ++   +   ++GS R KEK  ++++ ++    E    AE   W  +L
Sbjct: 429 MRFRGLASEAGAEEILTEIVESESGSGRLKEKASKILQTLRGGGSEFGEGAEAREWNRML 488

Query: 544 DSGCFGSRSRCRLGAGMDRSTANSSEF 555
           ++    SRS+ + G G     A SS+F
Sbjct: 489 EASGL-SRSQFQQG-GQKGGFAYSSQF 509

BLAST of CSPI05G28380 vs. TAIR 10
Match: AT5G67340.1 (ARM repeat superfamily protein )

HSP 1 Score: 165.2 bits (417), Expect = 1.5e-40
Identity = 140/474 (29.54%), Postives = 238/474 (50.21%), Query Frame = 0

Query: 65  IPKELLCPITGSLMADPVIVSSGHTFEAACVQVCKDLGVKPTLLDGSKPDFSSVIPNLAL 124
           +P +  C ++  LM DPVIV+SG TFE   +Q   D+G+            +++ PN  +
Sbjct: 240 VPSDFRCSLSLELMTDPVIVASGQTFERVFIQKWIDMGLMVCPKTRQALSHTTLTPNFIV 299

Query: 125 KSTIFNWCKNSSSEPPQPLDFSSAEK----LVRKFVAA-----HSKS--DEELIQ----- 184
           ++ + +WC+ ++  PP PL+   + +    LV    A+     HS+S   EEL Q     
Sbjct: 300 RAFLASWCETNNVYPPDPLELIHSSEPFPLLVESVRASSSENGHSESLDAEELRQVFSRS 359

Query: 185 ----GVAETPVV---RFNHAATEVARRSSHFHSSSDESVSAVVPTLPLPLAIRPSCCSSS 244
               G+    V    R N+AA + +   S+      E      P + +P  +R +   SS
Sbjct: 360 ASAPGIVSEVVCKTKRNNNAAADRSLTRSNTPWKFPEERHWRHPGI-IPATVRET--GSS 419

Query: 245 SSSDNEIIGTLNLPEEEEIVVKLKSSQVIEIEEAVTTLRKITRTREDSRVHLCSPMILSA 304
           SS + E+         ++++  LKSS +    EA   +R + R   D+R+ +     + +
Sbjct: 420 SSIETEV---------KKLIDDLKSSSLDTQREATARIRILARNSTDNRIVIARCEAIPS 479

Query: 305 LRSLIVSRYSGVQVNSVAALVNLSLENLNKVKIVRSGILPNLIDVLKGG-SPEVQEHAAG 364
           L SL+ S    +Q ++V  L+NLS+ + NK  I  SG +  LI VLK G   E + ++A 
Sbjct: 480 LVSLLYSTDERIQADAVTCLLNLSINDNNKSLIAESGAIVPLIHVLKTGYLEEAKANSAA 539

Query: 365 AIFSLALEDNNKTAIGVLGALPPLIRLLLSNSEQTRHDSALALYHLSHVQSNRSKLVKLG 424
            +FSL++ +  KT IG  GA+ PL+ LL S S   + D+A AL++LS    N++K+++ G
Sbjct: 540 TLFSLSVIEEYKTEIGEAGAIEPLVDLLGSGSLSGKKDAATALFNLSIHHENKTKVIEAG 599

Query: 425 SVPILLGMVKSRH-MAGRILLTLCNLAACFEGRAALLDSGAVECLVGMLRENELDSESTR 484
           +V  L+ ++     M  + ++ L NLA   EG+ A+ + G +  LV ++   EL S   +
Sbjct: 600 AVRYLVELMDPAFGMVEKAVVVLANLATVREGKIAIGEEGGIPVLVEVV---ELGSARGK 659

Query: 485 ESCVAVLFGLSFGGLRFKGLAKTAGAMDVFMAVEKNGSERSKEKVKRMMEYMKA 514
           E+  A L  L     +F       G +   +A+ K+G+ R KEK + +++Y KA
Sbjct: 660 ENATAALLQLCTHSPKFCNNVIREGVIPPLVALTKSGTARGKEKAQNLLKYFKA 698

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FL172.7e-14857.01U-box domain-containing protein 40 OS=Arabidopsis thaliana OX=3702 GN=PUB40 PE=2... [more]
Q9FJP63.7e-12149.19U-box domain-containing protein 38 OS=Arabidopsis thaliana OX=3702 GN=PUB38 PE=1... [more]
Q0WUF64.2e-10945.62U-box domain-containing protein 41 OS=Arabidopsis thaliana OX=3702 GN=PUB41 PE=2... [more]
Q9STT11.5e-10645.62U-box domain-containing protein 39 OS=Arabidopsis thaliana OX=3702 GN=PUB39 PE=1... [more]
Q5XEZ82.1e-3929.54U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A0A0KX662.7e-30899.82RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_5G631520 PE... [more]
A0A5A7T1Z72.7e-30097.29RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C8I31.4e-28896.49RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103498047 PE=4 ... [more]
A0A6J1K2N71.7e-27890.07RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111489531 P... [more]
A0A6J1FNQ02.2e-27890.25RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111445559... [more]
Match NameE-valueIdentityDescription
XP_011655976.15.5e-30899.82U-box domain-containing protein 40 [Cucumis sativus] >KGN52401.1 hypothetical pr... [more]
KAA0037472.15.5e-30097.29U-box domain-containing protein 40 [Cucumis melo var. makuwa] >TYK01948.1 U-box ... [more]
XP_008458723.12.8e-28896.49PREDICTED: U-box domain-containing protein 40 [Cucumis melo][more]
XP_038889155.11.2e-28391.34U-box domain-containing protein 40 [Benincasa hispida][more]
XP_022993578.13.5e-27890.07U-box domain-containing protein 40 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT5G40140.11.9e-14957.01RING/U-box superfamily protein with ARM repeat domain [more]
AT5G65200.12.6e-12249.19plant U-box 38 [more]
AT5G62560.13.0e-11045.62RING/U-box superfamily protein with ARM repeat domain [more]
AT3G47820.19.9e-10646.94PLANT U-BOX 39 [more]
AT5G67340.11.5e-4029.54ARM repeat superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000225ArmadilloSMARTSM00185arm_5coord: 266..306
e-value: 360.0
score: 1.2
coord: 428..471
e-value: 4.6
score: 15.9
coord: 307..347
e-value: 0.045
score: 22.9
coord: 389..427
e-value: 170.0
score: 3.7
coord: 348..388
e-value: 1.6
score: 17.7
IPR000225ArmadilloPFAMPF00514Armcoord: 310..346
e-value: 1.2E-4
score: 22.0
coord: 350..387
e-value: 1.2E-5
score: 25.1
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 318..355
score: 9.6424
IPR000225ArmadilloPROSITEPS50176ARM_REPEATcoord: 359..401
score: 9.9224
IPR003613U box domainSMARTSM00504Ubox_2coord: 68..131
e-value: 2.8E-8
score: 43.5
IPR003613U box domainPFAMPF04564U-boxcoord: 65..135
e-value: 2.5E-9
score: 37.2
IPR003613U box domainPROSITEPS51698U_BOXcoord: 64..138
score: 31.722446
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 64..137
e-value: 2.1E-15
score: 58.5
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 235..510
e-value: 8.3E-40
score: 138.6
NoneNo IPR availablePANTHERPTHR23315U BOX DOMAIN-CONTAININGcoord: 39..542
NoneNo IPR availablePANTHERPTHR23315:SF276U-BOX DOMAIN-CONTAINING PROTEIN 38coord: 39..542
NoneNo IPR availableCDDcd16453RING-Uboxcoord: 70..105
e-value: 6.22851E-11
score: 55.5864
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 60..139
IPR009465Spondin, N-terminalPROSITEPS51020SPONDINcoord: 1..81
score: 8.737619
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 243..490

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G28380.1CSPI05G28380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005515 protein binding
molecular_function GO:0004842 ubiquitin-protein transferase activity