CSPI05G21550 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G21550
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionProteasome subunit beta
LocationChr5: 22268649 .. 22272621 (-)
RNA-Seq ExpressionCSPI05G21550
SyntenyCSPI05G21550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCCCAAGAAACCGATTTACTTAAACCTTCACCCAAGTCCCAATGGCGAAAATCAAACAGTTACCGATTCGTTTCGTCCACCTCAGTCTTCTTCGTTCTCTACATACGCCTCCAATCCTTAACGGTATCGTGTACTTTTTCATTCTATTCTTTATGGTGGTGATTGTCGAAATTTTCGTTTAGTTTTCATTATGATCACTTCATTTCTTGTGTCACATTAAATTATGATTTGCAGCAATAGTTGAAGTATCAGTTTGGTTACTCAGACTATGTGGATAGAGGATTTGATAGTTAGCTGAGACTCTAATTTAATTTATGGGTTTATTTCTCTGTGCTCTTCATTGTTTGTTTATAATTGAGCGCTCAATGAAGTACGGAGGAGCTTTCGGGTTGGGATTAGATGATGTTAGATGGTTTTGGGAGTTCTTTTTGTATATTGTGGTTATTAATAGCCAATCTTTTAATTGGGTTCCTGTCACAAGATCTTGAGCATGAAGCTGGACACTAGCGGTCTTGAATCGGCTACTTTTCTCTCTGGCGCGGGCAGTACTGAGCTACTCGATGGATTATCCTCGGCGCCTTCTTTTGGTATTCCAAATGCTAGGAATGTGAGTGAGAATTATGTTATTCTTAGTTCTTATTGATTCTTTTTCTTTTGCAAAATGTTAACGCTGAACTTCGCTTTTCAGTTTGATGGGTTTCAGAAAGAGGCTATTCAGATGGTGAAGCCAGCAAAGGGGACAACCACTCTGGCCTTCACTTTCAAGGAAGGAGTCATGGTTGCTGCTGATTCCCGAGCGAGCATGGGAGGGTACATATGTCAGTTCTGAAAGTCCTGAATTCTTTTACATATATATTTTGCCACTTGTTTCTCTAGATTTTCTTTATGTTCCTCTATTTCTTAATGTTCTAAGTGGATTTGATTTGAGTGCGAAACTATCATGTATAAAATTGTTAGATGTTGTCTGTCTATGTGAAGATGAATCTCCTTAGTTTGTTTTGGTACATCAGTATTCCATCTGAGAAACAACGCATAAACATCATTGCTTGGCATCTAGTGGTCAATGTATGTGAGAGAGGTAAAAAGGGTAAAGAGAAATTTAACTGTTAGTTAAGGGGAGTTGTGCCCTGCTTGTATGGGGAGGTTGCAATTGGAGAAAATTTGCTATCATTTTAGCTTAAAAAAAAACTGGGGGTGGTGTGGACTTAAACCAAGTTTTGGTTATTTTCTACATTGACTGACACTCATATACCTAACTACTTACATTTAGCTTAGTTGTGTACTTACTGTGCAACATCTACCCTTTCACTATGCTATCTGCTACTGAATAATTCATTTATGTTTTCTGATATTTTCAGCATCCCAATCTGCGAAGAAGATTATTGAAATAAATTCATACATGCTTGGCACAATGGCTGGAGGAGCTGCTGATTGCCAGTTTTGGCACAGAAATCTTGGAATTAAGGTGATCTATGGACTCCTTTAGCATCAAAGGGTTATGAATTGTTATACACCTATTTTATCACAGCTTTCACAACTTGAACCCGACTATTTCCGTGACAATGAGATGCACATGAGTTTGTGTTTGTGTACTGTTTGCTCAGTCAAAATCCCTTAGGATAGGAATGGAATGAAAGTTGAAATTCCCATAGAAAGTCTATCAAGTTGGTTATTGATTGAAATAACACATATTTAGACATTTCCATGCCTTCCTTTTTTGGCAGAATCATTTTGTTGGTAAGGGGAGAAGTTAGCGTTTGGATTCCTATTGATTCCAGCCTTTCTTTTCTCTCAGCACCAATTAGAAAGGTCTTGTGACGTTATTTTTTCTTTTTAATTGGAAGAAAGGGAAGATTTTGGGCATTTAACTCTATGGTAGTTAACCAGACAGACTCAGGAGATATCCTTTTGGACTCTGCAAACTAATTGGTACCCATTCCGTTTTGATTTTACCATTCCAAGAATCTCACTGGTCTTCCTTCTGAAAAACATTAGTCATTGAAAGCCTGAAGGTTGGTTAATGGTTGGTGTCGAGGTTCATTTTTAATGATTGGTGACCTGGGTATTTGAGTGCATTCACTTGTTTTCCATGGTGAATTTGTGGCTAATAATCAGATTGGAACATTGAACAAATCTTCCTAGCTTGTGTATTCACGTGTTTTCCCTGGTGAATTCCAGAGCCATGAGATCTTGAATGTTTGAATTTTAATAGTTCATGAGAACGTCTTTTCTCTAGATATGATAGATTATTGCATTACTCTAATGTTTGGATTAACATGACATGACTAGTTTGAGTGATGGTGACGTTGACATTCATGTAGTGTATCTTTTCTATTGTAGGCTGTTAAACTTGATGTCATAAATTTACATTTTACATGATTTGCTGATGGAAATCGTTTGATACGATACAGTGTCGACGACATGAGTTGGAAAACAAGCGTAGAATTTCAGTGAAGGGTGCATCTAAGTTGCTAGCTAACATTCTATATTCGTATCGGGGAATGGGTCTTTCAGTTGGTACCATGATTGCTGGATGGGATGAAACTGTAAGTCTGTAATTTCTTCCCTAACGGATTTGATTAACGCTCTCTAGTAATTTAAGCTAAATTATCAGAACATATATTTATATCTTATGGGGTTGCAGGGTCCTGGATTGTACTATGTTGACAGTGAAGGAAGAAGACTGAAAGGAACTCGATTCTCAGTTGGATCAGGTTCACCTTATGCATATTGTGTCCTGGACAATGGGTAATCATCTTTGCTTATTACATCTGATTGGATACTTTTTACTGCAACAAATTCTTTAGAAATGCATAAAACACTGATTGAGTAAACTAAAAAGACACATATGTGAAATAATAGTAAATTTTGAGTGTGAAATACATTCTACATTAAACTAATTATTTTATAAGCATATAAATGCATCGATCCATTTACTTAAAATTTTCTTCTAGTATAAAAATATATATTTTTAAATATGTATCCCTTTATGCTTCTTAGCATATGCTTCAGTTTCAACAAATTAGAAACTTGTAACCTACATTATTGGTCTGTCATTCCTTTATGAATTGGTAATTTCTTGACTATGCCAAAGTTTAGAATTAACTGGGTTGTTTCTTGGGATTTAATTACTCTCTGCTTCTACTTGGTCTTCTACTTCTAAATTCTTTTGTAACTATACCCTAGCTCATATTTTTCCACCAGTTGGGCTTTTCTTTTTTTATGCCCGGTTGGTGTTTGGAGATATCTCATTCCCCTCTTTTATATCTTCCTTTTGATAATGAAAGTTAAGTTTCTTAAAATATGCCAAACTTAAACTTGGATTCAAGATATTCTAATTTCCAACATATGTGCAGTTATCAGTACGATTTATCAGTTGAAGAGGCTGCTGAACTGGCTAGAAGAGCAATTTATCATGCAACATTCCGAGACGGGGCCAGTGGTGGAGTTGCGAGTGGTATCTCCTGATTGCTCCTCTCATTCTCAAGTTGATATGCATATTGTGTATTGATTTCTGGTTTACAAGCATATATTTATTTGAAACTTGTCCTTTGTTCAGTTTATCATGTTGGACCAAATGGATGGAAGAAACTATCTGGTGATGATGTTGGAGAACTTCACTACAAATATTATCCTGTTGTGCATGATACAGTTGAACAGGAAATGATTGAAGTCGCCGTGTAGATCTTTTTAACAAATGGATCCCAGGTTTCACCTTCTCTCCAAACAAACTACTTGATGTATTTTGTTAACTAGCAAACACATTTCGCTTTAGAAAATTACTTAGATGCATCTTATTTTATGCATCATACCAAATTGTCCGATCATAATCTGTCATGCTGTAAATTCATCTCACGTCTTCTTTAAATATTTTTATTTTCTCTTTTGTATGTGTGTGATGAGAGCAGGGTAGCGATAGCACTCAATTATTGCTCTTTGTTTGGTTGAAAATCTATGTACTATAGTGCTTTTA

mRNA sequence

GCCCAAGAAACCGATTTACTTAAACCTTCACCCAAGTCCCAATGGCGAAAATCAAACAGTTACCGATTCGTTTCGTCCACCTCAGTCTTCTTCGTTCTCTACATACGCCTCCAATCCTTAACGATCTTGAGCATGAAGCTGGACACTAGCGGTCTTGAATCGGCTACTTTTCTCTCTGGCGCGGGCAGTACTGAGCTACTCGATGGATTATCCTCGGCGCCTTCTTTTGGTATTCCAAATGCTAGGAATTTTGATGGGTTTCAGAAAGAGGCTATTCAGATGGTGAAGCCAGCAAAGGGGACAACCACTCTGGCCTTCACTTTCAAGGAAGGAGTCATGGTTGCTGCTGATTCCCGAGCGAGCATGGGAGGGTACATATCATCCCAATCTGCGAAGAAGATTATTGAAATAAATTCATACATGCTTGGCACAATGGCTGGAGGAGCTGCTGATTGCCAGTTTTGGCACAGAAATCTTGGAATTAAGTGTCGACGACATGAGTTGGAAAACAAGCGTAGAATTTCAGTGAAGGGTGCATCTAAGTTGCTAGCTAACATTCTATATTCGTATCGGGGAATGGGTCTTTCAGTTGGTACCATGATTGCTGGATGGGATGAAACTGGTCCTGGATTGTACTATGTTGACAGTGAAGGAAGAAGACTGAAAGGAACTCGATTCTCAGTTGGATCAGGTTCACCTTATGCATATTGTGTCCTGGACAATGGTTATCAGTACGATTTATCAGTTGAAGAGGCTGCTGAACTGGCTAGAAGAGCAATTTATCATGCAACATTCCGAGACGGGGCCAGTGGTGGAGTTGCGAGTGTTTATCATGTTGGACCAAATGGATGGAAGAAACTATCTGGTGATGATGTTGGAGAACTTCACTACAAATATTATCCTGTTGTGCATGATACAGTTGAACAGGAAATGATTGAAGTCGCCGTGTAGATCTTTTTAACAAATGGATCCCAGGTTTCACCTTCTCTCCAAACAAACTACTTGATGTATTTTGTTAACTAGCAAACACATTTCGCTTTAGAAAATTACTTAGATGCATCTTATTTTATGCATCATACCAAATTGTCCGATCATAATCTGTCATGCTGTAAATTCATCTCACGTCTTCTTTAAATATTTTTATTTTCTCTTTTGTATGTGTGTGATGAGAGCAGGGTAGCGATAGCACTCAATTATTGCTCTTTGTTTGGTTGAAAATCTATGTACTATAGTGCTTTTA

Coding sequence (CDS)

ATGAAGCTGGACACTAGCGGTCTTGAATCGGCTACTTTTCTCTCTGGCGCGGGCAGTACTGAGCTACTCGATGGATTATCCTCGGCGCCTTCTTTTGGTATTCCAAATGCTAGGAATTTTGATGGGTTTCAGAAAGAGGCTATTCAGATGGTGAAGCCAGCAAAGGGGACAACCACTCTGGCCTTCACTTTCAAGGAAGGAGTCATGGTTGCTGCTGATTCCCGAGCGAGCATGGGAGGGTACATATCATCCCAATCTGCGAAGAAGATTATTGAAATAAATTCATACATGCTTGGCACAATGGCTGGAGGAGCTGCTGATTGCCAGTTTTGGCACAGAAATCTTGGAATTAAGTGTCGACGACATGAGTTGGAAAACAAGCGTAGAATTTCAGTGAAGGGTGCATCTAAGTTGCTAGCTAACATTCTATATTCGTATCGGGGAATGGGTCTTTCAGTTGGTACCATGATTGCTGGATGGGATGAAACTGGTCCTGGATTGTACTATGTTGACAGTGAAGGAAGAAGACTGAAAGGAACTCGATTCTCAGTTGGATCAGGTTCACCTTATGCATATTGTGTCCTGGACAATGGTTATCAGTACGATTTATCAGTTGAAGAGGCTGCTGAACTGGCTAGAAGAGCAATTTATCATGCAACATTCCGAGACGGGGCCAGTGGTGGAGTTGCGAGTGTTTATCATGTTGGACCAAATGGATGGAAGAAACTATCTGGTGATGATGTTGGAGAACTTCACTACAAATATTATCCTGTTGTGCATGATACAGTTGAACAGGAAATGATTGAAGTCGCCGTGTAG

Protein sequence

MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTLAFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCRRHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGTRFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGWKKLSGDDVGELHYKYYPVVHDTVEQEMIEVAV*
Homology
BLAST of CSPI05G21550 vs. ExPASy Swiss-Prot
Match: O24361 (Proteasome subunit beta type-5 OS=Spinacia oleracea OX=3562 PE=2 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 1.6e-125
Identity = 223/270 (82.59%), Postives = 241/270 (89.26%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLES   +    S  + DGL   PSF +PN  +FDGFQKEA+QMVKPAKGTTTL
Sbjct: 1   MKLDTSGLESTAPIF-RRSDFVFDGLQMTPSFDLPNPTDFDGFQKEAVQMVKPAKGTTTL 60

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AF FK GVMVAADSRASMGGYISSQS KKIIEIN YMLGTMAGGAADCQFWHRNLGIKCR
Sbjct: 61  AFIFKHGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCR 120

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
            HEL NKRRISV GASKLLANILY+YRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKG 
Sbjct: 121 LHELANKRRISVTGASKLLANILYNYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGM 180

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAY VLDNGY+YD++VEEA+ELARRAIYHAT+RDGASGGV SVYHVGP+GW
Sbjct: 181 RFSVGSGSPYAYGVLDNGYKYDMTVEEASELARRAIYHATYRDGASGGVVSVYHVGPDGW 240

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEV 271
           KK++GDDVG+LH++YYPVV  TVEQEM+EV
Sbjct: 241 KKVTGDDVGDLHFQYYPVVPATVEQEMVEV 269

BLAST of CSPI05G21550 vs. ExPASy Swiss-Prot
Match: Q9LIP2 (Proteasome subunit beta type-5-B OS=Arabidopsis thaliana OX=3702 GN=PBE2 PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 2.7e-125
Identity = 224/272 (82.35%), Postives = 241/272 (88.60%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGS-TELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSGLE+   + G GS +E+LDG SSAPSF +P   +FDGFQK+A++MVKPAKGTTT
Sbjct: 1   MKLDTSGLETTMPVIGFGSNSEMLDGFSSAPSFDLPRTTDFDGFQKKAVEMVKPAKGTTT 60

Query: 61  LAFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKC 120
           LAF FKEGVMVAADSRASMGGYISSQS KKIIEIN YMLGTMAGGAADCQFWHRNLGIKC
Sbjct: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKG 180
           R HEL NKRRISV GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EG RLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNG 240
            RFSVGSGSPYAY VLD+GY++D+SVEEA+ELARR+IYHATFRDGASGGVASVYHVGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPQG 240

Query: 241 WKKLSGDDVGELHYKYYPVVHDTVEQEMIEVA 272
           W KLSGDDVGELHY YYPV   T E  M E A
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPITAEHVMEEAA 272

BLAST of CSPI05G21550 vs. ExPASy Swiss-Prot
Match: O23717 (Proteasome subunit beta type-5-A OS=Arabidopsis thaliana OX=3702 GN=PBE1 PE=1 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 1.4e-121
Identity = 219/270 (81.11%), Postives = 236/270 (87.41%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAG-STELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG E++  + G G S+++LD LSS PSF +P  + FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKC 120
           LAF FK GVMVAADSRASMGGYISSQS KKIIEIN YMLGTMAGGAADCQFWHRNLGIKC
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKG 180
           R HEL NKRRISV GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EG RLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNG 240
            RFSVGSGSPYAY VLD+GY+YD+SVEEA+ELARR+IYHATFRDGASGGVASVYHVGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPEG 240

Query: 241 WKKLSGDDVGELHYKYYPVVHDTVEQEMIE 270
           W KLSGDDVGELHY YYPV   T EQ M E
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPATAEQVMEE 270

BLAST of CSPI05G21550 vs. ExPASy Swiss-Prot
Match: P28074 (Proteasome subunit beta type-5 OS=Homo sapiens OX=9606 GN=PSMB5 PE=1 SV=3)

HSP 1 Score: 291.6 bits (745), Expect = 9.3e-78
Identity = 149/250 (59.60%), Postives = 182/250 (72.80%), Query Frame = 0

Query: 7   GLESATFLSGAGSTELLDGLS-SAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTLAFTFK 66
           GL     L   G   L DGLS +AP +G+P        ++  I+M+    GTTTLAF F+
Sbjct: 20  GLGGRADLLDLGPGSLSDGLSLAAPGWGVP--------EEPGIEML---HGTTTLAFKFR 79

Query: 67  EGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCRRHELE 126
            GV+VAADSRA+ G YI+SQ+ KK+IEIN Y+LGTMAGGAADC FW R L  +CR +EL 
Sbjct: 80  HGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELR 139

Query: 127 NKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGTRFSVG 186
           NK RISV  ASKLLAN++Y Y+GMGLS+GTMI GWD+ GPGLYYVDSEG R+ G  FSVG
Sbjct: 140 NKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVG 199

Query: 187 SGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGWKKLSG 246
           SGS YAY V+D GY YDL VE+A +LARRAIY AT+RD  SGG  ++YHV  +GW ++S 
Sbjct: 200 SGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSS 258

Query: 247 DDVGELHYKY 256
           D+V +LH KY
Sbjct: 260 DNVADLHEKY 258

BLAST of CSPI05G21550 vs. ExPASy Swiss-Prot
Match: Q5R8S2 (Proteasome subunit beta type-5 OS=Pongo abelii OX=9601 GN=PSMB5 PE=2 SV=3)

HSP 1 Score: 291.6 bits (745), Expect = 9.3e-78
Identity = 149/250 (59.60%), Postives = 182/250 (72.80%), Query Frame = 0

Query: 7   GLESATFLSGAGSTELLDGLS-SAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTLAFTFK 66
           GL     L   G   L DGLS +AP +G+P        ++  I+M+    GTTTLAF F+
Sbjct: 20  GLGGRADLLDLGPGSLSDGLSLAAPGWGVP--------EEPGIEML---HGTTTLAFKFR 79

Query: 67  EGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCRRHELE 126
            GV+VAADSRA+ G YI+SQ+ KK+IEIN Y+LGTMAGGAADC FW R L  +CR +EL 
Sbjct: 80  HGVIVAADSRATAGAYIASQTVKKVIEINPYLLGTMAGGAADCSFWERLLARQCRIYELR 139

Query: 127 NKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGTRFSVG 186
           NK RISV  ASKLLAN++Y Y+GMGLS+GTMI GWD+ GPGLYYVDSEG R+ G  FSVG
Sbjct: 140 NKERISVAAASKLLANMVYQYKGMGLSMGTMICGWDKRGPGLYYVDSEGNRISGATFSVG 199

Query: 187 SGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGWKKLSG 246
           SGS YAY V+D GY YDL VE+A +LARRAIY AT+RD  SGG  ++YHV  +GW ++S 
Sbjct: 200 SGSVYAYGVMDRGYSYDLEVEQAYDLARRAIYQATYRDAYSGGAVNLYHVREDGWIRVSS 258

Query: 247 DDVGELHYKY 256
           D+V +LH KY
Sbjct: 260 DNVADLHEKY 258

BLAST of CSPI05G21550 vs. ExPASy TrEMBL
Match: A0A0A0KS11 (Proteasome subunit beta OS=Cucumis sativus OX=3659 GN=Csa_5G590010 PE=3 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 2.5e-150
Identity = 269/272 (98.90%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL
Sbjct: 17  MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 76

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR
Sbjct: 77  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 136

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
           RHELENKRRISV GASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKGT
Sbjct: 137 RHELENKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGT 196

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW
Sbjct: 197 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 256

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVAV 273
           KKLSGDDVGELHYKYYPVVHDTVEQEMIEV V
Sbjct: 257 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVTV 288

BLAST of CSPI05G21550 vs. ExPASy TrEMBL
Match: A0A6J1FZ24 (Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111449218 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 6.4e-138
Identity = 246/271 (90.77%), Postives = 257/271 (94.83%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLESAT+ SG+ STELLDGLS+ PSF +PNA NFDGFQK+AIQMVKPAKGTTTL
Sbjct: 1   MKLDTSGLESATYFSGSSSTELLDGLSTVPSFDVPNAANFDGFQKDAIQMVKPAKGTTTL 60

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AF FKEGVMVAADSRASMGGYISSQS KKIIEIN YMLGTMAGGAADCQFWHRNLGIKCR
Sbjct: 61  AFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCR 120

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
            HEL NKRRISV GASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKGT
Sbjct: 121 LHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGT 180

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAY VLDNGY+YDLS+EEAAELARRAIYHATFRDGASGGVASVY+VGPNGW
Sbjct: 181 RFSVGSGSPYAYGVLDNGYRYDLSIEEAAELARRAIYHATFRDGASGGVASVYYVGPNGW 240

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVA 272
           KKLSGDDVGELH+KYYPVV DTVEQEM+E+A
Sbjct: 241 KKLSGDDVGELHFKYYPVVADTVEQEMVEIA 271

BLAST of CSPI05G21550 vs. ExPASy TrEMBL
Match: A0A6J1DEC4 (Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111019662 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.1e-137
Identity = 249/271 (91.88%), Postives = 256/271 (94.46%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLESA+FLSGA +TELLD LS+APSF IPNA NFDGFQKEAIQMVKPAKGTTTL
Sbjct: 1   MKLDTSGLESASFLSGASNTELLDELSTAPSFDIPNATNFDGFQKEAIQMVKPAKGTTTL 60

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AF FKEGVMVAADSRASMGGYISSQS KK+IEIN YMLGTMAGGAADCQFWH NLGIKCR
Sbjct: 61  AFIFKEGVMVAADSRASMGGYISSQSVKKVIEINPYMLGTMAGGAADCQFWHGNLGIKCR 120

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
            HEL NKRRISV GASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKGT
Sbjct: 121 LHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGT 180

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAY VLDNGY+YD+ VEEAAELARRAIYHATFRDGASGGVASVY VGPNGW
Sbjct: 181 RFSVGSGSPYAYGVLDNGYRYDMLVEEAAELARRAIYHATFRDGASGGVASVYLVGPNGW 240

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVA 272
           KKLSGDDVGELHYKYYPVVHDTVEQEM+EVA
Sbjct: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMVEVA 271

BLAST of CSPI05G21550 vs. ExPASy TrEMBL
Match: A0A6J1DFM4 (Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111019662 PE=3 SV=1)

HSP 1 Score: 499.2 bits (1284), Expect = 1.1e-137
Identity = 249/271 (91.88%), Postives = 256/271 (94.46%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLESA+FLSGA +TELLD LS+APSF IPNA NFDGFQKEAIQMVKPAKGTTTL
Sbjct: 19  MKLDTSGLESASFLSGASNTELLDELSTAPSFDIPNATNFDGFQKEAIQMVKPAKGTTTL 78

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AF FKEGVMVAADSRASMGGYISSQS KK+IEIN YMLGTMAGGAADCQFWH NLGIKCR
Sbjct: 79  AFIFKEGVMVAADSRASMGGYISSQSVKKVIEINPYMLGTMAGGAADCQFWHGNLGIKCR 138

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
            HEL NKRRISV GASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKGT
Sbjct: 139 LHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGT 198

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAY VLDNGY+YD+ VEEAAELARRAIYHATFRDGASGGVASVY VGPNGW
Sbjct: 199 RFSVGSGSPYAYGVLDNGYRYDMLVEEAAELARRAIYHATFRDGASGGVASVYLVGPNGW 258

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVA 272
           KKLSGDDVGELHYKYYPVVHDTVEQEM+EVA
Sbjct: 259 KKLSGDDVGELHYKYYPVVHDTVEQEMVEVA 289

BLAST of CSPI05G21550 vs. ExPASy TrEMBL
Match: A0A6J1HQU8 (Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111466533 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 4.1e-137
Identity = 244/271 (90.04%), Postives = 257/271 (94.83%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLESAT+ SG+ STELL+GLS+ PSF +PNA NFDGFQK+AIQMVKPAKGTTTL
Sbjct: 1   MKLDTSGLESATYFSGSSSTELLNGLSTVPSFDVPNAANFDGFQKDAIQMVKPAKGTTTL 60

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AF FKEGVMVAADSRASMGGYISSQS KKIIEIN YMLGTMAGGAADCQFWHRNLGIKCR
Sbjct: 61  AFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCR 120

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
            HEL NKRRISV GASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKGT
Sbjct: 121 LHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGT 180

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAY VLDNGY+YD+S+EEAAELARRAIYHATFRDGASGGVASVY+VGPNGW
Sbjct: 181 RFSVGSGSPYAYGVLDNGYRYDISIEEAAELARRAIYHATFRDGASGGVASVYYVGPNGW 240

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVA 272
           KKLSGDDVGELH+KYYPVV DTVEQEM+E+A
Sbjct: 241 KKLSGDDVGELHFKYYPVVADTVEQEMVEIA 271

BLAST of CSPI05G21550 vs. NCBI nr
Match: XP_004135309.1 (proteasome subunit beta type-5 [Cucumis sativus] >KAE8648681.1 hypothetical protein Csa_007769 [Cucumis sativus])

HSP 1 Score: 541.2 bits (1393), Expect = 5.2e-150
Identity = 269/272 (98.90%), Postives = 269/272 (98.90%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL
Sbjct: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR
Sbjct: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
           RHELENKRRISV GASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKGT
Sbjct: 121 RHELENKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGT 180

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW
Sbjct: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVAV 273
           KKLSGDDVGELHYKYYPVVHDTVEQEMIEV V
Sbjct: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVTV 272

BLAST of CSPI05G21550 vs. NCBI nr
Match: XP_022944784.1 (proteasome subunit beta type-5-like [Cucurbita moschata])

HSP 1 Score: 500.0 bits (1286), Expect = 1.3e-137
Identity = 246/271 (90.77%), Postives = 257/271 (94.83%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLESAT+ SG+ STELLDGLS+ PSF +PNA NFDGFQK+AIQMVKPAKGTTTL
Sbjct: 1   MKLDTSGLESATYFSGSSSTELLDGLSTVPSFDVPNAANFDGFQKDAIQMVKPAKGTTTL 60

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AF FKEGVMVAADSRASMGGYISSQS KKIIEIN YMLGTMAGGAADCQFWHRNLGIKCR
Sbjct: 61  AFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCR 120

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
            HEL NKRRISV GASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKGT
Sbjct: 121 LHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGT 180

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAY VLDNGY+YDLS+EEAAELARRAIYHATFRDGASGGVASVY+VGPNGW
Sbjct: 181 RFSVGSGSPYAYGVLDNGYRYDLSIEEAAELARRAIYHATFRDGASGGVASVYYVGPNGW 240

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVA 272
           KKLSGDDVGELH+KYYPVV DTVEQEM+E+A
Sbjct: 241 KKLSGDDVGELHFKYYPVVADTVEQEMVEIA 271

BLAST of CSPI05G21550 vs. NCBI nr
Match: XP_022151757.1 (proteasome subunit beta type-5-like isoform X2 [Momordica charantia])

HSP 1 Score: 499.2 bits (1284), Expect = 2.3e-137
Identity = 249/271 (91.88%), Postives = 256/271 (94.46%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLESA+FLSGA +TELLD LS+APSF IPNA NFDGFQKEAIQMVKPAKGTTTL
Sbjct: 1   MKLDTSGLESASFLSGASNTELLDELSTAPSFDIPNATNFDGFQKEAIQMVKPAKGTTTL 60

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AF FKEGVMVAADSRASMGGYISSQS KK+IEIN YMLGTMAGGAADCQFWH NLGIKCR
Sbjct: 61  AFIFKEGVMVAADSRASMGGYISSQSVKKVIEINPYMLGTMAGGAADCQFWHGNLGIKCR 120

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
            HEL NKRRISV GASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKGT
Sbjct: 121 LHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGT 180

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAY VLDNGY+YD+ VEEAAELARRAIYHATFRDGASGGVASVY VGPNGW
Sbjct: 181 RFSVGSGSPYAYGVLDNGYRYDMLVEEAAELARRAIYHATFRDGASGGVASVYLVGPNGW 240

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVA 272
           KKLSGDDVGELHYKYYPVVHDTVEQEM+EVA
Sbjct: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMVEVA 271

BLAST of CSPI05G21550 vs. NCBI nr
Match: XP_022151756.1 (proteasome subunit beta type-5-like isoform X1 [Momordica charantia])

HSP 1 Score: 499.2 bits (1284), Expect = 2.3e-137
Identity = 249/271 (91.88%), Postives = 256/271 (94.46%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLESA+FLSGA +TELLD LS+APSF IPNA NFDGFQKEAIQMVKPAKGTTTL
Sbjct: 19  MKLDTSGLESASFLSGASNTELLDELSTAPSFDIPNATNFDGFQKEAIQMVKPAKGTTTL 78

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AF FKEGVMVAADSRASMGGYISSQS KK+IEIN YMLGTMAGGAADCQFWH NLGIKCR
Sbjct: 79  AFIFKEGVMVAADSRASMGGYISSQSVKKVIEINPYMLGTMAGGAADCQFWHGNLGIKCR 138

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
            HEL NKRRISV GASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKGT
Sbjct: 139 LHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGT 198

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAY VLDNGY+YD+ VEEAAELARRAIYHATFRDGASGGVASVY VGPNGW
Sbjct: 199 RFSVGSGSPYAYGVLDNGYRYDMLVEEAAELARRAIYHATFRDGASGGVASVYLVGPNGW 258

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVA 272
           KKLSGDDVGELHYKYYPVVHDTVEQEM+EVA
Sbjct: 259 KKLSGDDVGELHYKYYPVVHDTVEQEMVEVA 289

BLAST of CSPI05G21550 vs. NCBI nr
Match: XP_022966996.1 (proteasome subunit beta type-5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 497.3 bits (1279), Expect = 8.6e-137
Identity = 244/271 (90.04%), Postives = 257/271 (94.83%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGSTELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTTL 60
           MKLDTSGLESAT+ SG+ STELL+GLS+ PSF +PNA NFDGFQK+AIQMVKPAKGTTTL
Sbjct: 1   MKLDTSGLESATYFSGSSSTELLNGLSTVPSFDVPNAANFDGFQKDAIQMVKPAKGTTTL 60

Query: 61  AFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKCR 120
           AF FKEGVMVAADSRASMGGYISSQS KKIIEIN YMLGTMAGGAADCQFWHRNLGIKCR
Sbjct: 61  AFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKCR 120

Query: 121 RHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKGT 180
            HEL NKRRISV GASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEG RLKGT
Sbjct: 121 LHELANKRRISVTGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGGRLKGT 180

Query: 181 RFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNGW 240
           RFSVGSGSPYAY VLDNGY+YD+S+EEAAELARRAIYHATFRDGASGGVASVY+VGPNGW
Sbjct: 181 RFSVGSGSPYAYGVLDNGYRYDISIEEAAELARRAIYHATFRDGASGGVASVYYVGPNGW 240

Query: 241 KKLSGDDVGELHYKYYPVVHDTVEQEMIEVA 272
           KKLSGDDVGELH+KYYPVV DTVEQEM+E+A
Sbjct: 241 KKLSGDDVGELHFKYYPVVADTVEQEMVEIA 271

BLAST of CSPI05G21550 vs. TAIR 10
Match: AT3G26340.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 449.5 bits (1155), Expect = 1.9e-126
Identity = 224/272 (82.35%), Postives = 241/272 (88.60%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAGS-TELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSGLE+   + G GS +E+LDG SSAPSF +P   +FDGFQK+A++MVKPAKGTTT
Sbjct: 1   MKLDTSGLETTMPVIGFGSNSEMLDGFSSAPSFDLPRTTDFDGFQKKAVEMVKPAKGTTT 60

Query: 61  LAFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKC 120
           LAF FKEGVMVAADSRASMGGYISSQS KKIIEIN YMLGTMAGGAADCQFWHRNLGIKC
Sbjct: 61  LAFIFKEGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKG 180
           R HEL NKRRISV GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EG RLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNG 240
            RFSVGSGSPYAY VLD+GY++D+SVEEA+ELARR+IYHATFRDGASGGVASVYHVGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKFDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPQG 240

Query: 241 WKKLSGDDVGELHYKYYPVVHDTVEQEMIEVA 272
           W KLSGDDVGELHY YYPV   T E  M E A
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPITAEHVMEEAA 272

BLAST of CSPI05G21550 vs. TAIR 10
Match: AT1G13060.1 (20S proteasome beta subunit E1 )

HSP 1 Score: 437.2 bits (1123), Expect = 9.8e-123
Identity = 219/270 (81.11%), Postives = 236/270 (87.41%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAG-STELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG E++  + G G S+++LD LSS PSF +P  + FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNLGIKC 120
           LAF FK GVMVAADSRASMGGYISSQS KKIIEIN YMLGTMAGGAADCQFWHRNLGIKC
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISSQSVKKIIEINPYMLGTMAGGAADCQFWHRNLGIKC 120

Query: 121 RRHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGRRLKG 180
           R HEL NKRRISV GASKLLAN+LYSYRGMGLSVGTMIAGWDETGPGLYYVD+EG RLKG
Sbjct: 121 RLHELANKRRISVSGASKLLANMLYSYRGMGLSVGTMIAGWDETGPGLYYVDNEGGRLKG 180

Query: 181 TRFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYHVGPNG 240
            RFSVGSGSPYAY VLD+GY+YD+SVEEA+ELARR+IYHATFRDGASGGVASVYHVGP G
Sbjct: 181 DRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRSIYHATFRDGASGGVASVYHVGPEG 240

Query: 241 WKKLSGDDVGELHYKYYPVVHDTVEQEMIE 270
           W KLSGDDVGELHY YYPV   T EQ M E
Sbjct: 241 WTKLSGDDVGELHYHYYPVAPATAEQVMEE 270

BLAST of CSPI05G21550 vs. TAIR 10
Match: AT1G13060.2 (20S proteasome beta subunit E1 )

HSP 1 Score: 423.7 bits (1088), Expect = 1.1e-118
Identity = 219/294 (74.49%), Postives = 236/294 (80.27%), Query Frame = 0

Query: 1   MKLDTSGLESATFLSGAG-STELLDGLSSAPSFGIPNARNFDGFQKEAIQMVKPAKGTTT 60
           MKLDTSG E++  + G G S+++LD LSS PSF +P  + FDGFQK+A  M+K AKGTTT
Sbjct: 1   MKLDTSGFETSMPMIGFGSSSDMLDELSSVPSFDLPRTKEFDGFQKKAKDMLKHAKGTTT 60

Query: 61  LAFTFKEGVMVAADSRASMGGYIS------------------------SQSAKKIIEINS 120
           LAF FK GVMVAADSRASMGGYIS                        SQS KKIIEIN 
Sbjct: 61  LAFIFKGGVMVAADSRASMGGYISVSSLKNSSKNMHPFDIVDFGRNTTSQSVKKIIEINP 120

Query: 121 YMLGTMAGGAADCQFWHRNLGIKCRRHELENKRRISVKGASKLLANILYSYRGMGLSVGT 180
           YMLGTMAGGAADCQFWHRNLGIKCR HEL NKRRISV GASKLLAN+LYSYRGMGLSVGT
Sbjct: 121 YMLGTMAGGAADCQFWHRNLGIKCRLHELANKRRISVSGASKLLANMLYSYRGMGLSVGT 180

Query: 181 MIAGWDETGPGLYYVDSEGRRLKGTRFSVGSGSPYAYCVLDNGYQYDLSVEEAAELARRA 240
           MIAGWDETGPGLYYVD+EG RLKG RFSVGSGSPYAY VLD+GY+YD+SVEEA+ELARR+
Sbjct: 181 MIAGWDETGPGLYYVDNEGGRLKGDRFSVGSGSPYAYGVLDSGYKYDMSVEEASELARRS 240

Query: 241 IYHATFRDGASGGVASVYHVGPNGWKKLSGDDVGELHYKYYPVVHDTVEQEMIE 270
           IYHATFRDGASGGVASVYHVGP GW KLSGDDVGELHY YYPV   T EQ M E
Sbjct: 241 IYHATFRDGASGGVASVYHVGPEGWTKLSGDDVGELHYHYYPVAPATAEQVMEE 294

BLAST of CSPI05G21550 vs. TAIR 10
Match: AT4G31300.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 4.1e-20
Identity = 57/185 (30.81%), Postives = 99/185 (53.51%), Query Frame = 0

Query: 56  GTTTLAFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNL 115
           GTT +  T+  GV++ ADSR S G Y++++++ KI ++   +    +G AAD Q     +
Sbjct: 12  GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 71

Query: 116 GIKCRRHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGR 175
                +H +++ +  +VK ++ L+  + Y+ + M L  G ++ GWD+   G  Y    G 
Sbjct: 72  RYFLHQHTIQHGQPATVKVSANLIRMLAYNNKNM-LQTGLIVGGWDKYEGGKIYGIPLGG 131

Query: 176 RLKGTRFSV-GSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYH 235
            +    F++ GSGS Y Y   D  ++ +++ EEA +L  +A+  A  RDGASGGV     
Sbjct: 132 TVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVI 191

Query: 236 VGPNG 240
           +   G
Sbjct: 192 INSEG 195

BLAST of CSPI05G21550 vs. TAIR 10
Match: AT4G31300.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 4.1e-20
Identity = 56/185 (30.27%), Postives = 98/185 (52.97%), Query Frame = 0

Query: 56  GTTTLAFTFKEGVMVAADSRASMGGYISSQSAKKIIEINSYMLGTMAGGAADCQFWHRNL 115
           GTT +  T+  GV++ ADSR S G Y++++++ KI ++   +    +G AAD Q     +
Sbjct: 12  GTTIIGVTYNGGVVLGADSRTSTGMYVANRASDKITQLTDNVYVCRSGSAADSQVVSDYV 71

Query: 116 GIKCRRHELENKRRISVKGASKLLANILYSYRGMGLSVGTMIAGWDETGPGLYYVDSEGR 175
                +H +++ +  +VK ++ L+  + Y+ +   L  G ++ GWD+   G  Y    G 
Sbjct: 72  RYFLHQHTIQHGQPATVKVSANLIRMLAYNNKQNMLQTGLIVGGWDKYEGGKIYGIPLGG 131

Query: 176 RLKGTRFSV-GSGSPYAYCVLDNGYQYDLSVEEAAELARRAIYHATFRDGASGGVASVYH 235
            +    F++ GSGS Y Y   D  ++ +++ EEA +L  +A+  A  RDGASGGV     
Sbjct: 132 TVVEQPFAIGGSGSSYLYGFFDQAWKDNMTKEEAEQLVVKAVSLAIARDGASGGVVRTVI 191

Query: 236 VGPNG 240
           +   G
Sbjct: 192 INSEG 196

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O243611.6e-12582.59Proteasome subunit beta type-5 OS=Spinacia oleracea OX=3562 PE=2 SV=1[more]
Q9LIP22.7e-12582.35Proteasome subunit beta type-5-B OS=Arabidopsis thaliana OX=3702 GN=PBE2 PE=1 SV... [more]
O237171.4e-12181.11Proteasome subunit beta type-5-A OS=Arabidopsis thaliana OX=3702 GN=PBE1 PE=1 SV... [more]
P280749.3e-7859.60Proteasome subunit beta type-5 OS=Homo sapiens OX=9606 GN=PSMB5 PE=1 SV=3[more]
Q5R8S29.3e-7859.60Proteasome subunit beta type-5 OS=Pongo abelii OX=9601 GN=PSMB5 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A0A0KS112.5e-15098.90Proteasome subunit beta OS=Cucumis sativus OX=3659 GN=Csa_5G590010 PE=3 SV=1[more]
A0A6J1FZ246.4e-13890.77Proteasome subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111449218 PE=3 SV=1[more]
A0A6J1DEC41.1e-13791.88Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111019662 PE=3 SV=1[more]
A0A6J1DFM41.1e-13791.88Proteasome subunit beta OS=Momordica charantia OX=3673 GN=LOC111019662 PE=3 SV=1[more]
A0A6J1HQU84.1e-13790.04Proteasome subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111466533 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004135309.15.2e-15098.90proteasome subunit beta type-5 [Cucumis sativus] >KAE8648681.1 hypothetical prot... [more]
XP_022944784.11.3e-13790.77proteasome subunit beta type-5-like [Cucurbita moschata][more]
XP_022151757.12.3e-13791.88proteasome subunit beta type-5-like isoform X2 [Momordica charantia][more]
XP_022151756.12.3e-13791.88proteasome subunit beta type-5-like isoform X1 [Momordica charantia][more]
XP_022966996.18.6e-13790.04proteasome subunit beta type-5-like isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G26340.11.9e-12682.35N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT1G13060.19.8e-12381.1120S proteasome beta subunit E1 [more]
AT1G13060.21.1e-11874.4920S proteasome beta subunit E1 [more]
AT4G31300.14.1e-2030.81N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
AT4G31300.24.1e-2030.27N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000243Peptidase T1A, proteasome beta-subunitPRINTSPR00141PROTEASOMEcoord: 221..232
score: 53.98
coord: 196..207
score: 44.89
coord: 64..79
score: 58.24
coord: 185..196
score: 63.83
IPR001353Proteasome, subunit alpha/betaPFAMPF00227Proteasomecoord: 55..235
e-value: 5.4E-48
score: 163.0
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 57..266
e-value: 1.0E-73
score: 249.7
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 49..255
NoneNo IPR availablePANTHERPTHR11599PROTEASOME SUBUNIT ALPHA/BETAcoord: 30..269
NoneNo IPR availablePANTHERPTHR11599:SF154PROTEASOME SUBUNIT BETAcoord: 30..269
NoneNo IPR availableCDDcd03761proteasome_beta_type_5coord: 57..244
e-value: 5.39417E-126
score: 354.243
IPR016050Proteasome beta-type subunit, conserved sitePROSITEPS00854PROTEASOME_BETA_1coord: 60..107
IPR023333Proteasome B-type subunitPROSITEPS51476PROTEASOME_BETA_2coord: 56..235
score: 53.542328

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G21550.1CSPI05G21550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010498 proteasomal protein catabolic process
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0019774 proteasome core complex, beta-subunit complex
cellular_component GO:0005839 proteasome core complex
molecular_function GO:0004298 threonine-type endopeptidase activity