CSPI05G20100 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G20100
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionprotein MIZU-KUSSEI 1-like
LocationChr5: 21289559 .. 21290548 (+)
RNA-Seq ExpressionCSPI05G20100
SyntenyCSPI05G20100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACCAAGTGTGTTTATATTAAACTCACAAAGCTCCACCTTCCATTGCTCATTAAGTCTTACCCACCAGGACAAGACAAAGACATACAAGAAAGATCATCATCTTTGTTTCTGTCTTTGTTTAACTTTCCATCTTATTATTTTTTCTTTTTCTGAAGATGAAAACGATTATGGCCAATTCTTCTTCACATGATTCCTTCTCCTTCTCCAAGAGATATAATCATTATTTCAACTTCAACAAAAACACCAAACACAACCATAACCAACACCAAGATCAAGAAGATGACCACCACCAAATCCTAACTGTCAACCCTCCAAAACACAAGCACACCACTGCTGTCTCCGTCTCCAAGCTCCGTTCCGCCATTGCATTATCGTTCGGGATTAGGACTCGGTCGTGTCGAGTCCTGGGGACGATATTCGGTCATAGACGAGGGCACGTGCATTTCTCGGTACAAACAGAGGGGAGTGCGAAGCCGATGTTCTTAGTGGAGCTAGCGATGCCGACGACTGCGCTCGTTCGAGAAATGGCGTCGGGGGTGGCGAGGATTGCGTTGGAGTGCGAGAGAGGGGAGAAAAAGAAGAAGAAGGGAGAGTTGAACGAGGAAGGGATATGGAGAGCATATTGTAATGGGAAGAAGTATGGAGTTGCTCATAGGTTTGAGTGTGGGGCTGAAGAATGGAGGATTCTTAGAGCTGTTGGGCCTATAACTGTTGGAGCTGGGGTGTTGCCTGGGATTGAAGAGGGAGGTTGTGAAGGTGAAGTGATGTTCATGAGGGCTAGGTTTGAGAGAGTCGTGGGTTCGAAGGATTCAGAAGCTTTTTACATGATTAACCCTGATGGGGTTGGTGGCCCTGAGCTTAGTATTTTTTTGCTCAGGGTTTGATTCGTTTCTCTCGCAAGAATAGTACTTGGAGTATAGTTTGGGTTACAGTAATCTTCATCCATATACATGACAAGTACTTCTGGTCTCATACTTGAAAGAGCTGCA

mRNA sequence

AACCAAGTGTGTTTATATTAAACTCACAAAGCTCCACCTTCCATTGCTCATTAAGTCTTACCCACCAGGACAAGACAAAGACATACAAGAAAGATCATCATCTTTGTTTCTGTCTTTGTTTAACTTTCCATCTTATTATTTTTTCTTTTTCTGAAGATGAAAACGATTATGGCCAATTCTTCTTCACATGATTCCTTCTCCTTCTCCAAGAGATATAATCATTATTTCAACTTCAACAAAAACACCAAACACAACCATAACCAACACCAAGATCAAGAAGATGACCACCACCAAATCCTAACTGTCAACCCTCCAAAACACAAGCACACCACTGCTGTCTCCGTCTCCAAGCTCCGTTCCGCCATTGCATTATCGTTCGGGATTAGGACTCGGTCGTGTCGAGTCCTGGGGACGATATTCGGTCATAGACGAGGGCACGTGCATTTCTCGGTACAAACAGAGGGGAGTGCGAAGCCGATGTTCTTAGTGGAGCTAGCGATGCCGACGACTGCGCTCGTTCGAGAAATGGCGTCGGGGGTGGCGAGGATTGCGTTGGAGTGCGAGAGAGGGGAGAAAAAGAAGAAGAAGGGAGAGTTGAACGAGGAAGGGATATGGAGAGCATATTGTAATGGGAAGAAGTATGGAGTTGCTCATAGGTTTGAGTGTGGGGCTGAAGAATGGAGGATTCTTAGAGCTGTTGGGCCTATAACTGTTGGAGCTGGGGTGTTGCCTGGGATTGAAGAGGGAGGTTGTGAAGGTGAAGTGATGTTCATGAGGGCTAGGTTTGAGAGAGTCGTGGGTTCGAAGGATTCAGAAGCTTTTTACATGATTAACCCTGATGGGGTTGGTGGCCCTGAGCTTAGTATTTTTTTGCTCAGGGTTTGATTCGTTTCTCTCGCAAGAATAGTACTTGGAGTATAGTTTGGGTTACAGTAATCTTCATCCATATACATGACAAGTACTTCTGGTCTCATACTTGAAAGAGCTGCA

Coding sequence (CDS)

ATGAAAACGATTATGGCCAATTCTTCTTCACATGATTCCTTCTCCTTCTCCAAGAGATATAATCATTATTTCAACTTCAACAAAAACACCAAACACAACCATAACCAACACCAAGATCAAGAAGATGACCACCACCAAATCCTAACTGTCAACCCTCCAAAACACAAGCACACCACTGCTGTCTCCGTCTCCAAGCTCCGTTCCGCCATTGCATTATCGTTCGGGATTAGGACTCGGTCGTGTCGAGTCCTGGGGACGATATTCGGTCATAGACGAGGGCACGTGCATTTCTCGGTACAAACAGAGGGGAGTGCGAAGCCGATGTTCTTAGTGGAGCTAGCGATGCCGACGACTGCGCTCGTTCGAGAAATGGCGTCGGGGGTGGCGAGGATTGCGTTGGAGTGCGAGAGAGGGGAGAAAAAGAAGAAGAAGGGAGAGTTGAACGAGGAAGGGATATGGAGAGCATATTGTAATGGGAAGAAGTATGGAGTTGCTCATAGGTTTGAGTGTGGGGCTGAAGAATGGAGGATTCTTAGAGCTGTTGGGCCTATAACTGTTGGAGCTGGGGTGTTGCCTGGGATTGAAGAGGGAGGTTGTGAAGGTGAAGTGATGTTCATGAGGGCTAGGTTTGAGAGAGTCGTGGGTTCGAAGGATTCAGAAGCTTTTTACATGATTAACCCTGATGGGGTTGGTGGCCCTGAGCTTAGTATTTTTTTGCTCAGGGTTTGA

Protein sequence

MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTAVSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRAVGPITVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV*
Homology
BLAST of CSPI05G20100 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 1.9e-42
Identity = 90/172 (52.33%), Postives = 117/172 (68.02%), Query Frame = 0

Query: 82  RVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREMASGVARIALECERGEKK 141
           RV GT++GH+RGHV FSVQ    + P+ L++LAM T  LV+EM+SG+ RIALECE+  + 
Sbjct: 128 RVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEKRHRS 187

Query: 142 KKKGELNEEGIWRAYCNGKK--YGVAHRFECGAEEWRILRAVGPITVGAGVLP------- 201
             K  L +E  W  YCNG+K  Y V+    C   +WR+L  V  +TVGAGV+P       
Sbjct: 188 GTK--LFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTIDD 247

Query: 202 --GIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV 243
             G+  G   GE+++MR +FERVVGS+DSEAFYM+NPD  GGPELSIFLLR+
Sbjct: 248 VSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

BLAST of CSPI05G20100 vs. ExPASy TrEMBL
Match: A0A0A0KT21 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G577380 PE=4 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 3.8e-134
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0

Query: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTA 60
           MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTA
Sbjct: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTA 60

Query: 61  VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL 120
           VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL
Sbjct: 61  VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL 120

Query: 121 VREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRA 180
           VREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRA
Sbjct: 121 VREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRA 180

Query: 181 VGPITVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL 240
           VGPITVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL
Sbjct: 181 VGPITVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL 240

Query: 241 RV 243
           RV
Sbjct: 241 RV 242

BLAST of CSPI05G20100 vs. ExPASy TrEMBL
Match: A0A1S3CSZ4 (protein MIZU-KUSSEI 1-like OS=Cucumis melo OX=3656 GN=LOC103504606 PE=4 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 3.3e-130
Identity = 235/242 (97.11%), Postives = 240/242 (99.17%), Query Frame = 0

Query: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTA 60
           MKTIMANSSSHDSFSFSKRYN+YFNFNKNTKHNHNQHQ++EDDHHQILTVNPPKHKHTT 
Sbjct: 1   MKTIMANSSSHDSFSFSKRYNNYFNFNKNTKHNHNQHQEEEDDHHQILTVNPPKHKHTTV 60

Query: 61  VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL 120
           VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL
Sbjct: 61  VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL 120

Query: 121 VREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRA 180
           VREMASGVARIALECERGEKKKKKGELNEEGIWR+YCNGKKYG+AHRFECGAEEWRILRA
Sbjct: 121 VREMASGVARIALECERGEKKKKKGELNEEGIWRSYCNGKKYGIAHRFECGAEEWRILRA 180

Query: 181 VGPITVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL 240
           VGPITVGAGVLPGIEEGG EGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL
Sbjct: 181 VGPITVGAGVLPGIEEGGGEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL 240

Query: 241 RV 243
           RV
Sbjct: 241 RV 242

BLAST of CSPI05G20100 vs. ExPASy TrEMBL
Match: A0A5D3BMI5 (Protein MIZU-KUSSEI 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1728G00160 PE=4 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 2.0e-127
Identity = 230/238 (96.64%), Postives = 235/238 (98.74%), Query Frame = 0

Query: 5   MANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTAVSVS 64
           MANSSSHDSFSFSKRYN+YFNFNKNTKHNHNQHQ++EDDHHQILT+NPPKHKHTT  SVS
Sbjct: 1   MANSSSHDSFSFSKRYNNYFNFNKNTKHNHNQHQEEEDDHHQILTLNPPKHKHTTVASVS 60

Query: 65  KLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREM 124
           KLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREM
Sbjct: 61  KLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREM 120

Query: 125 ASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRAVGPI 184
           ASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYG+AHRFECGAEEWRILRAVGPI
Sbjct: 121 ASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGIAHRFECGAEEWRILRAVGPI 180

Query: 185 TVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV 243
           TVGAGVLPGIEEGG EGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV
Sbjct: 181 TVGAGVLPGIEEGGGEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV 238

BLAST of CSPI05G20100 vs. ExPASy TrEMBL
Match: A0A6J1IGY2 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111472829 PE=4 SV=1)

HSP 1 Score: 308.5 bits (789), Expect = 2.4e-80
Identity = 178/249 (71.49%), Postives = 195/249 (78.31%), Query Frame = 0

Query: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVN--PPKHKHT 60
           MKT+MA SS+  SFSFS+RY H+ N + N        Q   +D HQ+LT N   PKHKH 
Sbjct: 1   MKTMMAKSSNDSSFSFSRRYFHFKNKDSN--------QGGNEDDHQVLTFNHFTPKHKH- 60

Query: 61  TAVSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQ-TEGSAKPMFLVELAMPT 120
             VS+SKLRSAIA + G + RS RV+GTIFGHRRGHVHFSVQ  +G +KP FLVELAMPT
Sbjct: 61  GIVSISKLRSAIA-ALG-KARSDRVVGTIFGHRRGHVHFSVQPKKGDSKPTFLVELAMPT 120

Query: 121 TALVREMASGVARIALECERGEK--KKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEW 180
           TALVREMASG ARIALECER  K   +KK  L EE IWRAYCNGKKYGVA RFECGAEEW
Sbjct: 121 TALVREMASGAARIALECERWSKGEMRKKAVLQEEAIWRAYCNGKKYGVAQRFECGAEEW 180

Query: 181 RILRAVGPITVGAGVLP--GIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGP 240
           RILRAVGPITVGAGVLP  G  E   +GEVMFMRA FERVVGS+DSEAFYMINP+GVGGP
Sbjct: 181 RILRAVGPITVGAGVLPANGDVEEEEDGEVMFMRASFERVVGSRDSEAFYMINPEGVGGP 238

Query: 241 ELSIFLLRV 243
           ELSIFLLRV
Sbjct: 241 ELSIFLLRV 238

BLAST of CSPI05G20100 vs. ExPASy TrEMBL
Match: A0A6J1EW36 (protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436596 PE=4 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 1.2e-79
Identity = 176/249 (70.68%), Postives = 195/249 (78.31%), Query Frame = 0

Query: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVN--PPKHKHT 60
           MKT+MA SS+  SFS S+RY H+ N + N        Q   +D HQ+LT N   PKHKH 
Sbjct: 1   MKTMMAKSSNDSSFSLSRRYFHFKNKDSN--------QGGNEDDHQVLTFNHLTPKHKH- 60

Query: 61  TAVSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQT-EGSAKPMFLVELAMPT 120
             VS+SKLRSAIA + G + RS RV+GTIFGHRRGHVHFSVQ+ +G +KP FLVELAMPT
Sbjct: 61  GIVSISKLRSAIA-ALG-KARSDRVVGTIFGHRRGHVHFSVQSKKGDSKPTFLVELAMPT 120

Query: 121 TALVREMASGVARIALECERGEK--KKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEW 180
           TALVREMASG ARIALEC+R  K   +KK  L EE IWRAYCNGKKYGVA RFECGAEEW
Sbjct: 121 TALVREMASGAARIALECDRWSKGEMRKKAVLQEEAIWRAYCNGKKYGVAQRFECGAEEW 180

Query: 181 RILRAVGPITVGAGVLP--GIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGP 240
           RILRAVGPITVGAGVLP  G  E   +GEVMFMRA FERVVGS+DSEAFYMINP+GVGGP
Sbjct: 181 RILRAVGPITVGAGVLPANGDVEEEEDGEVMFMRANFERVVGSRDSEAFYMINPEGVGGP 238

Query: 241 ELSIFLLRV 243
           ELSIFLLRV
Sbjct: 241 ELSIFLLRV 238

BLAST of CSPI05G20100 vs. NCBI nr
Match: XP_004147139.2 (protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN51547.1 hypothetical protein Csa_007775 [Cucumis sativus])

HSP 1 Score: 487.3 bits (1253), Expect = 7.9e-134
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0

Query: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTA 60
           MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTA
Sbjct: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTA 60

Query: 61  VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL 120
           VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL
Sbjct: 61  VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL 120

Query: 121 VREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRA 180
           VREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRA
Sbjct: 121 VREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRA 180

Query: 181 VGPITVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL 240
           VGPITVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL
Sbjct: 181 VGPITVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL 240

Query: 241 RV 243
           RV
Sbjct: 241 RV 242

BLAST of CSPI05G20100 vs. NCBI nr
Match: XP_008467200.1 (PREDICTED: protein MIZU-KUSSEI 1-like [Cucumis melo])

HSP 1 Score: 474.2 bits (1219), Expect = 6.9e-130
Identity = 235/242 (97.11%), Postives = 240/242 (99.17%), Query Frame = 0

Query: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTA 60
           MKTIMANSSSHDSFSFSKRYN+YFNFNKNTKHNHNQHQ++EDDHHQILTVNPPKHKHTT 
Sbjct: 1   MKTIMANSSSHDSFSFSKRYNNYFNFNKNTKHNHNQHQEEEDDHHQILTVNPPKHKHTTV 60

Query: 61  VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL 120
           VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL
Sbjct: 61  VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL 120

Query: 121 VREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRA 180
           VREMASGVARIALECERGEKKKKKGELNEEGIWR+YCNGKKYG+AHRFECGAEEWRILRA
Sbjct: 121 VREMASGVARIALECERGEKKKKKGELNEEGIWRSYCNGKKYGIAHRFECGAEEWRILRA 180

Query: 181 VGPITVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL 240
           VGPITVGAGVLPGIEEGG EGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL
Sbjct: 181 VGPITVGAGVLPGIEEGGGEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL 240

Query: 241 RV 243
           RV
Sbjct: 241 RV 242

BLAST of CSPI05G20100 vs. NCBI nr
Match: TYK00275.1 (protein MIZU-KUSSEI 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 464.9 bits (1195), Expect = 4.2e-127
Identity = 230/238 (96.64%), Postives = 235/238 (98.74%), Query Frame = 0

Query: 5   MANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTAVSVS 64
           MANSSSHDSFSFSKRYN+YFNFNKNTKHNHNQHQ++EDDHHQILT+NPPKHKHTT  SVS
Sbjct: 1   MANSSSHDSFSFSKRYNNYFNFNKNTKHNHNQHQEEEDDHHQILTLNPPKHKHTTVASVS 60

Query: 65  KLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREM 124
           KLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREM
Sbjct: 61  KLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREM 120

Query: 125 ASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRAVGPI 184
           ASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYG+AHRFECGAEEWRILRAVGPI
Sbjct: 121 ASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGIAHRFECGAEEWRILRAVGPI 180

Query: 185 TVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV 243
           TVGAGVLPGIEEGG EGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV
Sbjct: 181 TVGAGVLPGIEEGGGEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV 238

BLAST of CSPI05G20100 vs. NCBI nr
Match: XP_038876009.1 (protein MIZU-KUSSEI 1-like [Benincasa hispida])

HSP 1 Score: 388.7 bits (997), Expect = 3.8e-104
Identity = 210/242 (86.78%), Postives = 219/242 (90.50%), Query Frame = 0

Query: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTA 60
           MKTIMA  SSHDSFSFS+RY  +FNFNK TKHN+N  QD EDD HQILT  PPK KH  A
Sbjct: 1   MKTIMA-KSSHDSFSFSRRY--HFNFNK-TKHNNNS-QDHEDD-HQILTFKPPKQKH-AA 60

Query: 61  VSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL 120
           VSVSKLRSAIAL  GIR+RSCRV+GTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL
Sbjct: 61  VSVSKLRSAIAL-LGIRSRSCRVVGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTAL 120

Query: 121 VREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRA 180
           VREMASGVARIALECERGEK+KKKG+LNEEGIWRAYCNGKKYGVAHRFECG EEWRIL+A
Sbjct: 121 VREMASGVARIALECERGEKRKKKGKLNEEGIWRAYCNGKKYGVAHRFECGGEEWRILKA 180

Query: 181 VGPITVGAGVLPGIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLL 240
           +GPITVGAGVLP   EG  EGEVMFMRA FERVVGSKDSEAFYMINPDGVGGPELSIFLL
Sbjct: 181 IGPITVGAGVLPESGEGCGEGEVMFMRASFERVVGSKDSEAFYMINPDGVGGPELSIFLL 234

Query: 241 RV 243
           RV
Sbjct: 241 RV 234

BLAST of CSPI05G20100 vs. NCBI nr
Match: XP_022974209.1 (protein MIZU-KUSSEI 1-like [Cucurbita maxima])

HSP 1 Score: 308.5 bits (789), Expect = 5.0e-80
Identity = 178/249 (71.49%), Postives = 195/249 (78.31%), Query Frame = 0

Query: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKNTKHNHNQHQDQEDDHHQILTVN--PPKHKHT 60
           MKT+MA SS+  SFSFS+RY H+ N + N        Q   +D HQ+LT N   PKHKH 
Sbjct: 1   MKTMMAKSSNDSSFSFSRRYFHFKNKDSN--------QGGNEDDHQVLTFNHFTPKHKH- 60

Query: 61  TAVSVSKLRSAIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQ-TEGSAKPMFLVELAMPT 120
             VS+SKLRSAIA + G + RS RV+GTIFGHRRGHVHFSVQ  +G +KP FLVELAMPT
Sbjct: 61  GIVSISKLRSAIA-ALG-KARSDRVVGTIFGHRRGHVHFSVQPKKGDSKPTFLVELAMPT 120

Query: 121 TALVREMASGVARIALECERGEK--KKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEW 180
           TALVREMASG ARIALECER  K   +KK  L EE IWRAYCNGKKYGVA RFECGAEEW
Sbjct: 121 TALVREMASGAARIALECERWSKGEMRKKAVLQEEAIWRAYCNGKKYGVAQRFECGAEEW 180

Query: 181 RILRAVGPITVGAGVLP--GIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGP 240
           RILRAVGPITVGAGVLP  G  E   +GEVMFMRA FERVVGS+DSEAFYMINP+GVGGP
Sbjct: 181 RILRAVGPITVGAGVLPANGDVEEEEDGEVMFMRASFERVVGSRDSEAFYMINPEGVGGP 238

Query: 241 ELSIFLLRV 243
           ELSIFLLRV
Sbjct: 241 ELSIFLLRV 238

BLAST of CSPI05G20100 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 206.1 bits (523), Expect = 3.3e-53
Identity = 131/271 (48.34%), Postives = 172/271 (63.47%), Query Frame = 0

Query: 1   MKTIMANSSSHDSFSFSKRYNHYFNFNKN--TKHNHNQHQDQEDDHH----QILT----- 60
           MK+I+AN+S   SFS SKR   YFN+ K    + +  + +++EDD++    ++LT     
Sbjct: 1   MKSILANTSLDSSFSLSKR---YFNWKKKKVQEDDDEEEEEEEDDYNHNEKKVLTRFNFS 60

Query: 61  VNPPKHKHTTAVSVSKLRS-------AIALSFGIRTRSCRVLGTIFGHRRGHVHFSVQTE 120
            +P +     A  + K +          AL F       RV+GT+FG+RRGHV+F+VQ +
Sbjct: 61  SDPTRPDQFNAQQIMKKKKKKTLEKIRYALGFSNSGLGFRVVGTLFGNRRGHVYFAVQDD 120

Query: 121 GSAKPMFLVELAMPTTALVREMASGVARIALECERGEKKKKKGELNEEGIWRAYCNGKKY 180
            +  P  L++L  PT+ LVREMASG+ RIALE    +   KK +L EE  WR YCNGKK 
Sbjct: 121 PTRLPAVLIQLPTPTSVLVREMASGLVRIALETAAYKTDSKK-KLLEESTWRTYCNGKKC 180

Query: 181 GVAHRFECGAEEWRILRAVGPITVGAGVLPGI-----EEG----GCE-GEVMFMRARFER 240
           G A R ECG  EW++L+AVGPIT+GAGVLP       EEG    G E GE+M+MRARFER
Sbjct: 181 GYAARKECGEAEWKVLKAVGPITMGAGVLPATTTTVDEEGNGAVGSEKGELMYMRARFER 240

Query: 241 VVGSKDSEAFYMINPD-GVGGPELSIFLLRV 243
           VVGS+DSEAFYM+NPD   GGPELS++ LRV
Sbjct: 241 VVGSRDSEAFYMMNPDVSSGGPELSVYFLRV 267

BLAST of CSPI05G20100 vs. TAIR 10
Match: AT5G23100.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 188.7 bits (478), Expect = 5.4e-48
Identity = 115/273 (42.12%), Postives = 163/273 (59.71%), Query Frame = 0

Query: 8   SSSHDSFSFSKRY----NHYFNFNKNTKHNHNQHQDQEDDHHQILTVNPPKHKHTTAVSV 67
           S  H+SF ++++     N   + +K   H++N              +   K   + A  V
Sbjct: 7   SKRHNSFHWTRKVGSDENDDVSSHKPLPHHNNTKPSSSSSSSSSNVITHKKKLQSFA--V 66

Query: 68  SKLRSAIALSFGIRTRS------CRVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPT 127
           S+LRS IA     R  +       RV+GT+FG RRGHVHFS+Q + ++ P FL+ELA P 
Sbjct: 67  SRLRSVIATLSRARPGNQNSGLGSRVVGTLFGSRRGHVHFSIQKDPNSPPAFLIELATPI 126

Query: 128 TALVREMASGVARIALECERGEKKKKKGELN------------------------EEGIW 187
           + LV+EMASG+ RIALEC++G+++++  E N                        EE +W
Sbjct: 127 SGLVKEMASGLVRIALECDKGKEEEEGEEKNGTLRHGGGDKTKTTTTAAVSRRLVEEPMW 186

Query: 188 RAYCNGKKYGVAHRFECGAEEWRILRAVGPITVGAGVLPGIEE----GGCEGEVMFMRAR 243
           R YCNGKK G A R ECG +E ++L+A+  +++GAGVLP  EE    GG  G++M+MRA+
Sbjct: 187 RTYCNGKKCGFATRRECGEKEKKVLKALEMVSMGAGVLPETEEIGGGGGGGGDIMYMRAK 246

BLAST of CSPI05G20100 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 174.1 bits (440), Expect = 1.4e-43
Identity = 90/172 (52.33%), Postives = 117/172 (68.02%), Query Frame = 0

Query: 82  RVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREMASGVARIALECERGEKK 141
           RV GT++GH+RGHV FSVQ    + P+ L++LAM T  LV+EM+SG+ RIALECE+  + 
Sbjct: 128 RVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALECEKRHRS 187

Query: 142 KKKGELNEEGIWRAYCNGKK--YGVAHRFECGAEEWRILRAVGPITVGAGVLP------- 201
             K  L +E  W  YCNG+K  Y V+    C   +WR+L  V  +TVGAGV+P       
Sbjct: 188 GTK--LFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAGVIPTPKTIDD 247

Query: 202 --GIEEGGCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV 243
             G+  G   GE+++MR +FERVVGS+DSEAFYM+NPD  GGPELSIFLLR+
Sbjct: 248 VSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLLRI 297

BLAST of CSPI05G20100 vs. TAIR 10
Match: AT4G39610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 156.8 bits (395), Expect = 2.3e-38
Identity = 78/169 (46.15%), Postives = 111/169 (65.68%), Query Frame = 0

Query: 82  RVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREMASGVARIALECER---- 141
           R+ GT+FG+R+G V  S+Q      P  +VELAM TT L +E+++G+ RIALE E+    
Sbjct: 96  RITGTLFGYRKGRVSLSIQENPKCLPSLVVELAMQTTTLQKELSTGMVRIALETEKQPRA 155

Query: 142 ---GEKKKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRAVGPITVGAGVLPGI 201
                K +KK ++ EE +W  YC G+K G   + E   E+  ++  + P+++GAGVLPG 
Sbjct: 156 DNNNSKTEKKTDILEEPLWTMYCKGEKTGYGVKREATEEDLNVMELLRPVSMGAGVLPGN 215

Query: 202 EEG-GCEGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV 243
            E  G +GE+ +MRA FERV+GSKDSE FYM++P+G  GPELS F +RV
Sbjct: 216 SESEGPDGEMAYMRAYFERVIGSKDSETFYMLSPEGNNGPELSFFFVRV 264

BLAST of CSPI05G20100 vs. TAIR 10
Match: AT2G21990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 150.2 bits (378), Expect = 2.1e-36
Identity = 79/163 (48.47%), Postives = 110/163 (67.48%), Query Frame = 0

Query: 82  RVLGTIFGHRRGHVHFSVQTEGSAKPMFLVELAMPTTALVREMASGVARIALECE-RGEK 141
           RV GT+FG+R+G V  S+Q      P  +VELAM T  L +E++ G+ RIALE E RG+K
Sbjct: 92  RVTGTLFGYRKGRVSLSIQESPRCLPSLVVELAMQTMVLQKELSGGMVRIALETEKRGDK 151

Query: 142 KKKKGELNEEGIWRAYCNGKKYGVAHRFECGAEEWRILRAVGPITVGAGVLPG-IEEGGC 201
           +K K  + +E +W  + NGKK G   + +   E+  ++  + P+++GAGVLPG  E  G 
Sbjct: 152 EKIK--IMDEPLWTMFSNGKKTGYGVKRDATEEDLNVMELLRPVSMGAGVLPGNTEFEGP 211

Query: 202 EGEVMFMRARFERVVGSKDSEAFYMINPDGVGGPELSIFLLRV 243
           + E+ +MRA FERVVGSKDSE FYM++P+G  GPELSIF +RV
Sbjct: 212 DSEMAYMRAYFERVVGSKDSETFYMLSPEGNNGPELSIFFVRV 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O222271.9e-4252.33Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KT213.8e-134100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G577380 PE=4 SV=1[more]
A0A1S3CSZ43.3e-13097.11protein MIZU-KUSSEI 1-like OS=Cucumis melo OX=3656 GN=LOC103504606 PE=4 SV=1[more]
A0A5D3BMI52.0e-12796.64Protein MIZU-KUSSEI 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A6J1IGY22.4e-8071.49protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111472829 PE=4 SV=1[more]
A0A6J1EW361.2e-7970.68protein MIZU-KUSSEI 1-like OS=Cucurbita moschata OX=3662 GN=LOC111436596 PE=4 SV... [more]
Match NameE-valueIdentityDescription
XP_004147139.27.9e-134100.00protein MIZU-KUSSEI 1 [Cucumis sativus] >KGN51547.1 hypothetical protein Csa_007... [more]
XP_008467200.16.9e-13097.11PREDICTED: protein MIZU-KUSSEI 1-like [Cucumis melo][more]
TYK00275.14.2e-12796.64protein MIZU-KUSSEI 1-like [Cucumis melo var. makuwa][more]
XP_038876009.13.8e-10486.78protein MIZU-KUSSEI 1-like [Benincasa hispida][more]
XP_022974209.15.0e-8071.49protein MIZU-KUSSEI 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT3G25640.13.3e-5348.34Protein of unknown function, DUF617 [more]
AT5G23100.15.4e-4842.12Protein of unknown function, DUF617 [more]
AT2G41660.11.4e-4352.33Protein of unknown function, DUF617 [more]
AT4G39610.12.3e-3846.15Protein of unknown function, DUF617 [more]
AT2G21990.12.1e-3648.47Protein of unknown function, DUF617 [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006460Protein MIZU-KUSSEI 1-like, plantPFAMPF04759DUF617coord: 83..241
e-value: 7.6E-64
score: 214.7
IPR006460Protein MIZU-KUSSEI 1-like, plantTIGRFAMTIGR01570TIGR01570coord: 83..242
e-value: 2.7E-61
score: 204.2
IPR006460Protein MIZU-KUSSEI 1-like, plantPANTHERPTHR31696PROTEIN MIZU-KUSSEI 1coord: 1..242
NoneNo IPR availablePANTHERPTHR31696:SF14OS01G0642600 PROTEINcoord: 1..242

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G20100.1CSPI05G20100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010274 hydrotropism