CSPI05G14670 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G14670
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionpolygalacturonase-like
LocationChr5: 15210988 .. 15214072 (-)
RNA-Seq ExpressionCSPI05G14670
SyntenyCSPI05G14670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTCTTGTTGGAAACTTACCCCTTCTTTCCCTTCTGTTTCTATTTCTTAACCATGGTTTGGCTGCTGGATCGACATTCAACATCGTTAGTCTCGGAGCCATAGCTGATGGCAAGACGGACGCCTCACATGCATTTCAAGAGGCATGGGCCAACGCCTGTGGCTCATCAGAACCGGCCACCATTTACGTGCCTAACGGTGCATTCTACATTCAGAGTGGGAACTTTAGTGGACCTTGTAAGAACAATGCTATTACTATTGTCATCAATGGCACTCTTATTGCTTCTTCAAATATTCAAGTTTTAGCTCAATCTCAAGCTTGGATAGCATTTAGACAAATAAATGGGCTTTCCATTTATGGTGGAGTTATCGATGGTCAAGGAATTGGGTTATGGAATTGTAAGCATTCAGGAAAGAGTTGCCCTGATGGAGCCACGGTATGCAAATAAATTTGAAACAAAAAAATGCAAGGTTGAATTTTGATTTGTACTGATTATTATTGTTCTTCATTTGTTTTGAATGTGTGTGCGTAGAATCTTGAAATCTCCCATGCACAAAATGTAAATGTGAATGGACTATCTTCCATTAATAGCCAAATGTTCAATATTGTGGTGTATGGTTGTGAAAATGTGCAAATCCAAGGAGTGAATGTCTCGTCTGCGGGCAATAGTCCAAACACTGATGGCATCCATGTCCAGCAATCATTGAACGTGAACATATCGAGCACAAGCATTGGCACTGGTGACGACTGCATTTCAATTGGTCCAGGAACTACCAACTTGTGGATGGAAAATATTAAATGTGGACCCGGACATGGAATCAGGTAAATTAAACTAGGAGTAGATGACAATAATTTATATGTTTGGTAAGGTTGATGAAAGTGTTACGATATTTTTATCATCTTTAAAATTGTTCCAACCATGCCATCAAATACTTAAGATCAATATCTATGAAACAAAATTTGAATTTGATGTGTATAGAAATAACAAAACAATGAGACCAACCAAAATGATTTTGGCCTTGTTTTTAAATAAAAAAATATGCTTCTTCCATCCAATATTTTAAGGTTGTTTTTTAATTATACAAAATATCATTAATAAATGTTGATACATAATGGTAGACTAATATCATCTATCTTTGATAGATGTTCGTAAATACTAACAAAACAACCCTTATCACTCTCCAATATTACTATATATATATATATTTAATATTTTTAGTAATTTTTATTTAAAACAATTTTATATATTTTTAAGTTTTCTACTTCATTAAAAAAAAAGCATTATAATTGAAAGCAAAGTTGGAAAAATCATTTTTAACTCAAATTCAGGTCGTTATTTCAAAAACATATTTTAAAAGAAGTATTAAAAGAAAAAGAAAAAGTGGTCTGTCATACTAAAAGGAAAAATTAATAGCTATGAATAGAAGTAATTTCGAAATTAAAGGACAATAATTATTATTTCCATATCTCAACAAACAAAATGGTGTGCATCAAATTTCCATATGGTGGTAAAATAAGATAAAACAGTCATTTTTTAATAAATAAAAATCTCTGCATGTCAAATTCTTATTTATCACTAAGTTTAATTGATTTTCATTCCTAGCAATTTCAAAATCGTTTTAAAAATATCCTAAACACACGATTCAAAAGTGATATTAACCCTTTCAAGAATAGTTGAAAAGAAGTTAAAAGTAGTTACACACGACATATTTTGTCATAAGCTTAGAGAAAATTTTTCATACCATCCTCGATTCATTACTTGTCCCCATCTATTAGCATTTAAGAAAAATATAAGTCCATAAATTAAATGAATATATATCATTGGATAACATATGTCAAATGATGAACGAGAGCAAAGTATGTGTTGTGTAATTGTAGCATTGGGAGCTTGGGGAGGCAAGTAGAAGAGGCTGGAGTGGAAAACGTGACGGTAACAACTGCAACTTTCACGGGGACTCAAAATGGAGTTAGGATTAAGTCTTGGGGAAGACCAAGCAATGCGTTCGCTACAAAAATTCTTTTTCAACACATTCGTTTCTATAATGTCAACAATCCTATCTTAATCGATCAAAATTACTGTCCAAATAACCAAGGCTGCCCTGGTCAGGTACGTTATTTATAGTTGTATCTATCATTGTGTAACTCATTTAGGGTATCGAAACACAAGTAACCATCATCTGTTTTTTCCTTTAAAGCTTGAAATAACTAATTTACTTTAGACGAAAATTTAGTAAGCAAATATATGCATTCGACATAACCCTTCATGTATCTCTGTAAAGGATGGATAATTGTCTATTTTAATTTAGCCATCATAAACTTTAATTTTTAATTACATTTCAAAGATTTTTGTTGAAGATATAATTGGCACGTCCTCACCTTGTAGTATAATTTCTCCTTGCATAGGAAAACATGACACTTTGAATGTTGCATTTGATTTGTATATATCATTGTTTCACTTTAGGGTTAGTTTATATGTTGCATTTTCTTGCTTCCAAGTTACCAAACATATATGAGGATTTTGCTTTTAAGAAACAAAATTTGAAGAACTAAAAAACAAAAACAAAAATTTAAATGGTTATCAAATAGAATGTTTTCCTCAACCTCTTACCATATTCTAATTTTTCAATTAACCCATCACAATTAAAATATTAGTTAACTTTCTTTTATTTTTTAGTTATGGTCCAAATACAAAATTTGAAATAAATCAATTTCAAATATTTATCGTAAATTGCATGCAAAGTGTTTAGTTTTAAATTATTCCTCAAATTTCAAATTACATAATTTAAAATCGTTTCAGGCGGATATGTATATCCTTGTTGATTCAATTTTCATAACATTGTGATTTTTGTGTGTGTGACTAGGCTTCCGGAGTTAAAGTTAGCGATGTGACCTATCAAGACGTTAACGGAACATCAGCCAGTGAAGTAGCAATCAATTTTGACTGTAGCCCAACAAATCCATGCACTGGAATAACGTTGGAAGACATACAACTCACTTACAACAATCAAATTCCAAAAGCTTCATGTGAAAATGCTCGTGGAACTGCCTCTGGTCCTCTTCAACCTCGGAGTTGCTTGACTTAG

mRNA sequence

ATGCCTCTTGTTGGAAACTTACCCCTTCTTTCCCTTCTGTTTCTATTTCTTAACCATGGTTTGGCTGCTGGATCGACATTCAACATCGTTAGTCTCGGAGCCATAGCTGATGGCAAGACGGACGCCTCACATGCATTTCAAGAGGCATGGGCCAACGCCTGTGGCTCATCAGAACCGGCCACCATTTACGTGCCTAACGGTGCATTCTACATTCAGAGTGGGAACTTTAGTGGACCTTGTAAGAACAATGCTATTACTATTGTCATCAATGGCACTCTTATTGCTTCTTCAAATATTCAAGTTTTAGCTCAATCTCAAGCTTGGATAGCATTTAGACAAATAAATGGGCTTTCCATTTATGGTGGAGTTATCGATGGTCAAGGAATTGGGTTATGGAATTGTAAGCATTCAGGAAAGAGTTGCCCTGATGGAGCCACGAATCTTGAAATCTCCCATGCACAAAATGTAAATGTGAATGGACTATCTTCCATTAATAGCCAAATGTTCAATATTGTGGTGTATGGTTGTGAAAATGTGCAAATCCAAGGAGTGAATGTCTCGTCTGCGGGCAATAGTCCAAACACTGATGGCATCCATGTCCAGCAATCATTGAACGTGAACATATCGAGCACAAGCATTGGCACTGGTGACGACTGCATTTCAATTGGTCCAGGAACTACCAACTTGTGGATGGAAAATATTAAATGTGGACCCGGACATGGAATCAGCATTGGGAGCTTGGGGAGGCAAGTAGAAGAGGCTGGAGTGGAAAACGTGACGGTAACAACTGCAACTTTCACGGGGACTCAAAATGGAGTTAGGATTAAGTCTTGGGGAAGACCAAGCAATGCGTTCGCTACAAAAATTCTTTTTCAACACATTCGTTTCTATAATGTCAACAATCCTATCTTAATCGATCAAAATTACTGTCCAAATAACCAAGGCTGCCCTGGTCAGGCTTCCGGAGTTAAAGTTAGCGATGTGACCTATCAAGACGTTAACGGAACATCAGCCAGTGAAGTAGCAATCAATTTTGACTGTAGCCCAACAAATCCATGCACTGGAATAACGTTGGAAGACATACAACTCACTTACAACAATCAAATTCCAAAAGCTTCATGTGAAAATGCTCGTGGAACTGCCTCTGGTCCTCTTCAACCTCGGAGTTGCTTGACTTAG

Coding sequence (CDS)

ATGCCTCTTGTTGGAAACTTACCCCTTCTTTCCCTTCTGTTTCTATTTCTTAACCATGGTTTGGCTGCTGGATCGACATTCAACATCGTTAGTCTCGGAGCCATAGCTGATGGCAAGACGGACGCCTCACATGCATTTCAAGAGGCATGGGCCAACGCCTGTGGCTCATCAGAACCGGCCACCATTTACGTGCCTAACGGTGCATTCTACATTCAGAGTGGGAACTTTAGTGGACCTTGTAAGAACAATGCTATTACTATTGTCATCAATGGCACTCTTATTGCTTCTTCAAATATTCAAGTTTTAGCTCAATCTCAAGCTTGGATAGCATTTAGACAAATAAATGGGCTTTCCATTTATGGTGGAGTTATCGATGGTCAAGGAATTGGGTTATGGAATTGTAAGCATTCAGGAAAGAGTTGCCCTGATGGAGCCACGAATCTTGAAATCTCCCATGCACAAAATGTAAATGTGAATGGACTATCTTCCATTAATAGCCAAATGTTCAATATTGTGGTGTATGGTTGTGAAAATGTGCAAATCCAAGGAGTGAATGTCTCGTCTGCGGGCAATAGTCCAAACACTGATGGCATCCATGTCCAGCAATCATTGAACGTGAACATATCGAGCACAAGCATTGGCACTGGTGACGACTGCATTTCAATTGGTCCAGGAACTACCAACTTGTGGATGGAAAATATTAAATGTGGACCCGGACATGGAATCAGCATTGGGAGCTTGGGGAGGCAAGTAGAAGAGGCTGGAGTGGAAAACGTGACGGTAACAACTGCAACTTTCACGGGGACTCAAAATGGAGTTAGGATTAAGTCTTGGGGAAGACCAAGCAATGCGTTCGCTACAAAAATTCTTTTTCAACACATTCGTTTCTATAATGTCAACAATCCTATCTTAATCGATCAAAATTACTGTCCAAATAACCAAGGCTGCCCTGGTCAGGCTTCCGGAGTTAAAGTTAGCGATGTGACCTATCAAGACGTTAACGGAACATCAGCCAGTGAAGTAGCAATCAATTTTGACTGTAGCCCAACAAATCCATGCACTGGAATAACGTTGGAAGACATACAACTCACTTACAACAATCAAATTCCAAAAGCTTCATGTGAAAATGCTCGTGGAACTGCCTCTGGTCCTCTTCAACCTCGGAGTTGCTTGACTTAG

Protein sequence

MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYGGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVNNPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYNNQIPKASCENARGTASGPLQPRSCLT*
Homology
BLAST of CSPI05G14670 vs. ExPASy Swiss-Prot
Match: P48979 (Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 2.6e-137
Identity = 234/384 (60.94%), Postives = 296/384 (77.08%), Query Frame = 0

Query: 10  LSLLFLF-LNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGA 69
           LSL+F+F +N  +A+  T+N+ SLGA ADGKTD++ AF  AWA AC S  P  IYVP G 
Sbjct: 10  LSLIFVFMINSAIASPLTYNVASLGAKADGKTDSTKAFLSAWAKACASMNPGVIYVPAGT 69

Query: 70  FYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYGGVIDGQG 129
           F+++   FSGPCKNNAIT  I GTL+A S+ +V+  +  WI F  +NG++I GG++DGQG
Sbjct: 70  FFLRDVVFSGPCKNNAITFRIAGTLVAPSDYRVIGNAANWIFFHHVNGVTISGGILDGQG 129

Query: 130 IGLWNCKH-SGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVS 189
             LW CK   G+SCP GAT L  S + N+ V+GL+S+NSQMF+IV+   +NVQ+QGV VS
Sbjct: 130 TALWACKACHGESCPSGATTLGFSDSNNIVVSGLASLNSQMFHIVINDFQNVQMQGVRVS 189

Query: 190 SAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSL 249
            +GNSPNTDGIHVQ S  V I ++ I TGDDC+SIGPGT+NLW+E + CGPGHGISIGSL
Sbjct: 190 RSGNSPNTDGIHVQMSSGVTILNSKIATGDDCVSIGPGTSNLWIEGVACGPGHGISIGSL 249

Query: 250 GRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVNNPILIDQ 309
           G++ EEAGV+NVTV T TF+GTQNG+RIKSWGRPS  FA  ILFQH    NV NPI+IDQ
Sbjct: 250 GKEQEEAGVQNVTVKTVTFSGTQNGLRIKSWGRPSTGFARNILFQHATMVNVENPIVIDQ 309

Query: 310 NYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYNN 369
           +YCP+N+GCPGQ SGV++SDVTY+D++GTSA+EVA+ FDCSP +PC  I LED++LTY N
Sbjct: 310 HYCPDNKGCPGQVSGVQISDVTYEDIHGTSATEVAVKFDCSPKHPCREIKLEDVKLTYKN 369

Query: 370 QIPKASCENARGTASGPLQPRSCL 392
           Q  ++SC +A GT  G +QP SCL
Sbjct: 370 QAAESSCSHADGTTEGVVQPTSCL 393

BLAST of CSPI05G14670 vs. ExPASy Swiss-Prot
Match: O22818 (Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g43860 PE=2 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 2.6e-105
Identity = 191/369 (51.76%), Postives = 261/369 (70.73%), Query Frame = 0

Query: 25  STFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGAFYIQSGNFSG-PCKNN 84
           ST N++S GA  DG  D++ AF  AW  AC S+ P TI VP G F + +  F G  CK  
Sbjct: 31  STLNVLSYGAKPDGSKDSTKAFLAAWDVACASANPTTIIVPKGRFLVGNLVFHGNECKQA 90

Query: 85  AITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYGGVIDGQGIGLWNCKHS-GKSCP 144
            I+I I G+++A  + +++A S+ WI F  +  +SIYGG++D QG  LW CK++ G +CP
Sbjct: 91  PISIRIAGSIVAPEDFRIIASSKHWIWFEDVTDVSIYGGILDAQGTSLWKCKNNGGHNCP 150

Query: 145 DGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSSAGNSPNTDGIHVQQ 204
            GA +L  S + N+ ++GL+SINSQ F+IV+    NV I GV VS+  NSPNTDGIHV+ 
Sbjct: 151 TGAKSLVFSGSNNIKISGLTSINSQKFHIVIDNSNNVNIDGVKVSADENSPNTDGIHVES 210

Query: 205 SLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSLGRQVEEAGVENVTVT 264
           S +V+I+++ IGTGDDCISIGPG+TN++++ I+CGPGHGISIGSLGR  EE GV+NVTV+
Sbjct: 211 SHSVHITNSRIGTGDDCISIGPGSTNVFIQTIRCGPGHGISIGSLGRAEEEQGVDNVTVS 270

Query: 265 TATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVNNPILIDQNYCPNNQGCPGQASG 324
              F GT NGVRIK+WG+ SN+FA  I+FQHI    V NPI+IDQ+YC  ++ CP Q SG
Sbjct: 271 NVDFMGTNNGVRIKTWGKDSNSFARNIVFQHINMKMVKNPIIIDQHYC-LHKPCPKQESG 330

Query: 325 VKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYNNQIPKASCENARGTAS 384
           VKVS+V Y+D++GTS +EVA+  DCS   PCTGI ++D+ L   ++  +ASC+NA G+A+
Sbjct: 331 VKVSNVRYEDIHGTSNTEVAVLLDCSKEKPCTGIVMDDVNLVSVHRPAQASCDNANGSAN 390

Query: 385 GPLQPRSCL 392
             +    CL
Sbjct: 391 DVVPFTPCL 398

BLAST of CSPI05G14670 vs. ExPASy Swiss-Prot
Match: P35339 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.5e-78
Identity = 155/373 (41.55%), Postives = 236/373 (63.27%), Query Frame = 0

Query: 24  GSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGAFYIQSGNFSGPCKNN 83
           G +F+I  LGA  +GKTD++ A QEAWA+ACG +   TI +P G F +   NF+GPCK +
Sbjct: 38  GGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGPLNFTGPCKGD 97

Query: 84  AITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYG-GVIDGQGIGLWNCKHSGK--S 143
            +TI +NG L+A++++        WI   +++ L I G G +DGQG  +W+     K   
Sbjct: 98  -VTIQVNGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGKLDGQGPAVWSKNSCVKKYD 157

Query: 144 CPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSSAGNSPNTDGIHV 203
           C     +L +    N  V+G++ +NS+ F++ +Y C+++ I+ VNV++ G+SPNTDGIH+
Sbjct: 158 CKILPNSLVMDFVNNGEVSGITLLNSKFFHMNMYKCKDMLIKDVNVTAPGDSPNTDGIHM 217

Query: 204 QQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSLGRQVEEAGVENVT 263
             S  V I++T IG GDDCISIGPGT+ + +  + CGPGHGISIGSLGR  +E  V ++ 
Sbjct: 218 GDSSGVTITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDIN 277

Query: 264 VTTATFTGTQNGVRIKSWGRPSNAF-ATKILFQHIRFYNVNNPILIDQNYCPNNQGCPGQ 323
           V   T   T NGVRIK++   ++   A+KI +++I+  +   PI+ID  YCPN       
Sbjct: 278 VKDCTLKKTANGVRIKAYEDAASVLTASKIHYENIKMEDSGYPIIIDMKYCPNKLCTANG 337

Query: 324 ASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYN--NQIPKASCENA 383
           AS V V DVT++++ GTS++  A+N  C+   PCTG+T++D+ + Y+  N    A C+NA
Sbjct: 338 ASKVTVKDVTFKNITGTSSTPEAVNLLCTAKIPCTGVTMDDVNIKYSGTNNKTMAVCKNA 397

Query: 384 RGTASGPLQPRSC 391
           +G+A G L+  +C
Sbjct: 398 KGSAKGCLKELAC 409

BLAST of CSPI05G14670 vs. ExPASy Swiss-Prot
Match: P35338 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 6.2e-75
Identity = 151/373 (40.48%), Postives = 231/373 (61.93%), Query Frame = 0

Query: 24  GSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGAFYIQSGNFSGPCKNN 83
           G +F+I  LGA  +GKTD++ A QEAWA+ACG +   TI +P G F +   NF+GPCK +
Sbjct: 38  GGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGD 97

Query: 84  AITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYG-GVIDGQGIGLWNCKHSGK--S 143
            +TI ++G L+A++++    +   WI   +++ L I G G +DGQG  +W+     K   
Sbjct: 98  -VTIQVDGNLLATTDLSQYKEHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYD 157

Query: 144 CPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSSAGNSPNTDGIHV 203
           C     +L +    N  V+G++ +NS+ F++ +Y C+N+ I+ V V++ G+SPNTDGIH+
Sbjct: 158 CKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYQCKNMLIKDVTVTAPGDSPNTDGIHM 217

Query: 204 QQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSLGRQVEEAGVENVT 263
             S  + I++T IG GDDCISIGPGT+ + +  + CGPGHGISIGSLGR  +E  V ++ 
Sbjct: 218 GDSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDIN 277

Query: 264 VTTATFTGTQNGVRIKSWGRPSNAF-ATKILFQHIRFYNVNNPILIDQNYCPNNQGCPGQ 323
           V   T   T  GVRIK++   ++    +KI +++I+  +  NPI ID  YCPN       
Sbjct: 278 VKDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANG 337

Query: 324 ASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYN--NQIPKASCENA 383
           AS V V DVT++++ GTS++  AI+  C+   PCTG T++D+ + Y+  N    A C NA
Sbjct: 338 ASKVTVKDVTFKNITGTSSTPEAISLLCTAKVPCTGATMDDVNVEYSGTNNKTMAICTNA 397

Query: 384 RGTASGPLQPRSC 391
           +G+  G L+  +C
Sbjct: 398 KGSTKGCLKELAC 409

BLAST of CSPI05G14670 vs. ExPASy Swiss-Prot
Match: P26216 (Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 1.8e-74
Identity = 149/373 (39.95%), Postives = 231/373 (61.93%), Query Frame = 0

Query: 24  GSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGAFYIQSGNFSGPCKNN 83
           G +F+I  LGA  +GKTD++ A QEAWA+ACG +   TI +P G F +   NF+GPCK +
Sbjct: 38  GGSFDITKLGASGNGKTDSTKAVQEAWASACGGTGKQTILIPKGDFLVGQLNFTGPCKGD 97

Query: 84  AITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYG-GVIDGQGIGLWNCKHSGK--S 143
            +TI ++G L+A++++        WI   +++ L I G G +DGQG  +W+     K   
Sbjct: 98  -VTIQVDGNLLATTDLSQYKDHGNWIEILRVDNLVITGKGNLDGQGPAVWSKNSCTKKYD 157

Query: 144 CPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSSAGNSPNTDGIHV 203
           C     +L +    N  V+G++ +NS+ F++ +Y C+++ I+ V V++ G+SPNTDGIH+
Sbjct: 158 CKILPNSLVMDFVNNGEVSGVTLLNSKFFHMNMYRCKDMLIKDVTVTAPGDSPNTDGIHM 217

Query: 204 QQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSLGRQVEEAGVENVT 263
             S  + I++T IG GDDCISIGPGT+ + +  + CGPGHGISIGSLGR  +E  V ++ 
Sbjct: 218 GDSSGITITNTVIGVGDDCISIGPGTSKVNITGVTCGPGHGISIGSLGRYKDEKDVTDIN 277

Query: 264 VTTATFTGTQNGVRIKSWGRPSNAF-ATKILFQHIRFYNVNNPILIDQNYCPNNQGCPGQ 323
           V   T   T  GVRIK++   ++    +KI +++I+  +  NPI ID  YCPN       
Sbjct: 278 VKDCTLKKTMFGVRIKAYEDAASVLTVSKIHYENIKMEDSANPIFIDMKYCPNKLCTANG 337

Query: 324 ASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYN--NQIPKASCENA 383
           AS V V DVT++++ GTS++  A++  C+   PCTG+T++D+ + Y+  N    A C NA
Sbjct: 338 ASKVTVKDVTFKNITGTSSTPEAVSLLCTAKVPCTGVTMDDVNVEYSGTNNKTMAICTNA 397

Query: 384 RGTASGPLQPRSC 391
           +G+  G L+  +C
Sbjct: 398 KGSTKGCLKELAC 409

BLAST of CSPI05G14670 vs. ExPASy TrEMBL
Match: A0A0A0KN91 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G409610 PE=3 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.4e-223
Identity = 388/391 (99.23%), Postives = 390/391 (99.74%), Query Frame = 0

Query: 1   MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA 60
           MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA
Sbjct: 1   MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA 60

Query: 61  TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY 120
           TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY
Sbjct: 61  TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY 120

Query: 121 GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ 180
           GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ
Sbjct: 121 GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ 180

Query: 181 IQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH 240
           IQGVNVSSAG+SPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH
Sbjct: 181 IQGVNVSSAGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH 240

Query: 241 GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVN 300
           GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFA KILFQHIRFYNVN
Sbjct: 241 GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFANKILFQHIRFYNVN 300

Query: 301 NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED 360
           NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED
Sbjct: 301 NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED 360

Query: 361 IQLTYNNQIPKASCENARGTASGPLQPRSCL 392
           IQLTYNNQIPKASC+NARGTASGPLQPRSCL
Sbjct: 361 IQLTYNNQIPKASCKNARGTASGPLQPRSCL 391

BLAST of CSPI05G14670 vs. ExPASy TrEMBL
Match: A0A5D3DS41 (Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold313G001940 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 2.9e-200
Identity = 343/391 (87.72%), Postives = 370/391 (94.63%), Query Frame = 0

Query: 1   MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA 60
           MPLVGNL LL LLFLFLNHGLAAGSTFNIVSLGA ADG TDAS AFQEAWANACGSSEPA
Sbjct: 1   MPLVGNLHLLFLLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEPA 60

Query: 61  TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY 120
           TIYVPNGAFY+ SGNF+GPCKNNAIT++INGTL+ASSNIQVLAQSQAWIAFR INGLSIY
Sbjct: 61  TIYVPNGAFYVHSGNFTGPCKNNAITVLINGTLMASSNIQVLAQSQAWIAFRHINGLSIY 120

Query: 121 GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ 180
           GGVIDGQG+GLWNCKHSG+SCPDGAT+LEI  +QNVNVNGLSS+NSQMFNIVVYGC+NVQ
Sbjct: 121 GGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNVQ 180

Query: 181 IQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH 240
           IQGVNVSSA +SPNTDGIHVQQSLNVNIS+++IGTGDDCISIGPGTTNLWMENI CGPGH
Sbjct: 181 IQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPGH 240

Query: 241 GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVN 300
           GISIGSLG+Q EEAGV+NVTVTTATF GTQNGVRIKSWGRPS+ FATKILFQHI FYNV+
Sbjct: 241 GISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNVS 300

Query: 301 NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED 360
           NPI IDQNYCP+ Q CPG+ASGVK+SDVTYQD+NGTSA+EVAINFDCSP+NPCTGI LED
Sbjct: 301 NPIFIDQNYCPHQQSCPGRASGVKISDVTYQDINGTSATEVAINFDCSPSNPCTGIHLED 360

Query: 361 IQLTYNNQIPKASCENARGTASGPLQPRSCL 392
           I L YNNQIP+ASCENA+GTASGPLQPRSCL
Sbjct: 361 INLNYNNQIPQASCENAQGTASGPLQPRSCL 391

BLAST of CSPI05G14670 vs. ExPASy TrEMBL
Match: A0A1S3CCA2 (polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103499335 PE=3 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 2.9e-200
Identity = 344/391 (87.98%), Postives = 370/391 (94.63%), Query Frame = 0

Query: 1   MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA 60
           MPLVGNL LL LLFLFLNHGLAAGSTFNIVSLGA ADG TDAS AFQEAWANACGSSEPA
Sbjct: 1   MPLVGNLHLLFLLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEPA 60

Query: 61  TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY 120
           TIYVPNGAFY+ SGNF+GPCKNNAITI+INGTL+ASSNIQVLAQSQAWIAFR INGLSIY
Sbjct: 61  TIYVPNGAFYVHSGNFTGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRHINGLSIY 120

Query: 121 GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ 180
           GGVIDGQG+GLWNCKHSG+SCPDGAT+LEI  +QNVNVNGLSS+NSQMFNIVVYGC+NVQ
Sbjct: 121 GGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNVQ 180

Query: 181 IQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH 240
           IQGVNVSSA +SPNTDGIHVQQSLNVNIS+++IGTGDDCISIGPGTTNLWMENI CGPGH
Sbjct: 181 IQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPGH 240

Query: 241 GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVN 300
           GISIGSLG+Q EEAGV+NVTVTTATF GTQNGVRIKSWGRPS+ FATKILFQHI FYNV+
Sbjct: 241 GISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNVS 300

Query: 301 NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED 360
           NPI IDQNYCP+ Q CPGQASGVK+SDVTYQD+NGTSA+EVAINFDCSP+NPCTGI LED
Sbjct: 301 NPIFIDQNYCPHQQSCPGQASGVKISDVTYQDINGTSATEVAINFDCSPSNPCTGIHLED 360

Query: 361 IQLTYNNQIPKASCENARGTASGPLQPRSCL 392
           I L YN+QIP+ASCENA+GTASGPLQPRSCL
Sbjct: 361 INLNYNDQIPQASCENAQGTASGPLQPRSCL 391

BLAST of CSPI05G14670 vs. ExPASy TrEMBL
Match: A0A6J1F3I5 (polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439547 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 5.5e-159
Identity = 275/393 (69.97%), Postives = 331/393 (84.22%), Query Frame = 0

Query: 1   MPLVGNLPLL--SLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSE 60
           M L G L L+   LL L LNH LA G TF+IVSLGA  DGKTDAS   Q AW  AC S+ 
Sbjct: 1   MALYGTLMLILSFLLLLLLNHVLADGLTFDIVSLGAKPDGKTDASSVLQTAWVKACDSTG 60

Query: 61  PATIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLS 120
           PATI+VP G FY+QSG+F+GPCKNNAITI I+GTL+ASS IQVLA+++AWI F+ +NGLS
Sbjct: 61  PATIHVPIGTFYVQSGSFNGPCKNNAITIRIDGTLVASSEIQVLAKTKAWIGFKDVNGLS 120

Query: 121 IYGGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCEN 180
           I+GGVIDG+G G+WNCKHSG +CPDGA NLEI +AQNV +NGL+SINSQ+FNIVV+GC N
Sbjct: 121 IHGGVIDGKGSGIWNCKHSGNNCPDGARNLEIINAQNVIINGLASINSQIFNIVVHGCRN 180

Query: 181 VQIQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGP 240
           V IQGV VS  G+SPNTDGIHVQ+S +V I S++IGTGDDCISIGPGTTNLW+E++ CGP
Sbjct: 181 VNIQGVKVSDPGDSPNTDGIHVQESSDVTILSSNIGTGDDCISIGPGTTNLWIESVTCGP 240

Query: 241 GHGISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYN 300
           GHGISIGSLGR  +E GV+NVTVT++TFTG+ NGVRIKSW RPSN FAT I FQHI   N
Sbjct: 241 GHGISIGSLGRSSDEPGVKNVTVTSSTFTGSTNGVRIKSWARPSNGFATDIHFQHITLDN 300

Query: 301 VNNPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITL 360
           V NPI+IDQNYCP+++GCP Q SGVK+S+VTY+D+ GTSA+E+ + FDC+PTNPCTG+TL
Sbjct: 301 VKNPIIIDQNYCPHDKGCPHQDSGVKISEVTYEDIKGTSATEIGVKFDCNPTNPCTGLTL 360

Query: 361 EDIQLTYNNQIPKASCENARGTASGPLQPRSCL 392
           EDI++TYN+QI KASCE+A+GTASG ++P SCL
Sbjct: 361 EDIKVTYNDQIAKASCESAKGTASGVVEPASCL 393

BLAST of CSPI05G14670 vs. ExPASy TrEMBL
Match: A0A6J1IEE1 (polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472451 PE=3 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 2.7e-158
Identity = 275/393 (69.97%), Postives = 331/393 (84.22%), Query Frame = 0

Query: 1   MPLVGNLP-LLSL-LFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSE 60
           M L G L  LLSL L L LNH LA G T +IVSLGA  DGKTD S   Q AWA AC S+ 
Sbjct: 1   MALYGTLMLLLSLSLLLLLNHALAGGLTLDIVSLGAKPDGKTDVSPVLQTAWAKACDSTG 60

Query: 61  PATIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLS 120
           PATI+VP G FY+QSG+F+GPCKNNAITI I+GTL+ASS+IQVLA+++AWI F+ +NGLS
Sbjct: 61  PATIHVPIGTFYVQSGSFNGPCKNNAITIRIDGTLVASSDIQVLAKTKAWIGFKDVNGLS 120

Query: 121 IYGGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCEN 180
           I+GGVIDG+G  +WNCKHSG +CPDGA NLEI++AQNV +NGL+SINSQ+FNIVVYGC+N
Sbjct: 121 IHGGVIDGKGSDIWNCKHSGNNCPDGAPNLEITNAQNVIINGLASINSQIFNIVVYGCQN 180

Query: 181 VQIQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGP 240
           V IQGV VS  G+SPNTDGIHVQ+S +V I S++IGTGDDCISIGPGTTNLW+E++ CGP
Sbjct: 181 VNIQGVKVSDPGDSPNTDGIHVQESSDVTILSSNIGTGDDCISIGPGTTNLWIESVTCGP 240

Query: 241 GHGISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYN 300
           GHGISIGSLG+  +E GV+NVTVT++TFT T NGVRIKSWGRPS+ FAT I FQHI   N
Sbjct: 241 GHGISIGSLGKSADEPGVKNVTVTSSTFTETTNGVRIKSWGRPSSGFATDIHFQHITLEN 300

Query: 301 VNNPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITL 360
           V NPI+IDQNYCP+++GCP Q SGVK+S+VTY+D+ GTSA+E+ + FDCSPTNPC G+TL
Sbjct: 301 VKNPIIIDQNYCPHDEGCPHQDSGVKISEVTYEDIKGTSATEIGVKFDCSPTNPCIGLTL 360

Query: 361 EDIQLTYNNQIPKASCENARGTASGPLQPRSCL 392
           EDI++TYNNQI KASC+ A+GTASG ++P SCL
Sbjct: 361 EDIKVTYNNQIAKASCKGAKGTASGVVEPASCL 393

BLAST of CSPI05G14670 vs. NCBI nr
Match: XP_004140371.1 (polygalacturonase [Cucumis sativus])

HSP 1 Score: 785.0 bits (2026), Expect = 3.0e-223
Identity = 388/391 (99.23%), Postives = 390/391 (99.74%), Query Frame = 0

Query: 1   MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA 60
           MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA
Sbjct: 1   MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA 60

Query: 61  TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY 120
           TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY
Sbjct: 61  TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY 120

Query: 121 GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ 180
           GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ
Sbjct: 121 GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ 180

Query: 181 IQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH 240
           IQGVNVSSAG+SPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH
Sbjct: 181 IQGVNVSSAGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH 240

Query: 241 GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVN 300
           GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFA KILFQHIRFYNVN
Sbjct: 241 GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFANKILFQHIRFYNVN 300

Query: 301 NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED 360
           NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED
Sbjct: 301 NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED 360

Query: 361 IQLTYNNQIPKASCENARGTASGPLQPRSCL 392
           IQLTYNNQIPKASC+NARGTASGPLQPRSCL
Sbjct: 361 IQLTYNNQIPKASCKNARGTASGPLQPRSCL 391

BLAST of CSPI05G14670 vs. NCBI nr
Match: XP_008460550.1 (PREDICTED: polygalacturonase-like [Cucumis melo])

HSP 1 Score: 707.6 bits (1825), Expect = 6.0e-200
Identity = 344/391 (87.98%), Postives = 370/391 (94.63%), Query Frame = 0

Query: 1   MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA 60
           MPLVGNL LL LLFLFLNHGLAAGSTFNIVSLGA ADG TDAS AFQEAWANACGSSEPA
Sbjct: 1   MPLVGNLHLLFLLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEPA 60

Query: 61  TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY 120
           TIYVPNGAFY+ SGNF+GPCKNNAITI+INGTL+ASSNIQVLAQSQAWIAFR INGLSIY
Sbjct: 61  TIYVPNGAFYVHSGNFTGPCKNNAITILINGTLMASSNIQVLAQSQAWIAFRHINGLSIY 120

Query: 121 GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ 180
           GGVIDGQG+GLWNCKHSG+SCPDGAT+LEI  +QNVNVNGLSS+NSQMFNIVVYGC+NVQ
Sbjct: 121 GGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNVQ 180

Query: 181 IQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH 240
           IQGVNVSSA +SPNTDGIHVQQSLNVNIS+++IGTGDDCISIGPGTTNLWMENI CGPGH
Sbjct: 181 IQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPGH 240

Query: 241 GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVN 300
           GISIGSLG+Q EEAGV+NVTVTTATF GTQNGVRIKSWGRPS+ FATKILFQHI FYNV+
Sbjct: 241 GISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNVS 300

Query: 301 NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED 360
           NPI IDQNYCP+ Q CPGQASGVK+SDVTYQD+NGTSA+EVAINFDCSP+NPCTGI LED
Sbjct: 301 NPIFIDQNYCPHQQSCPGQASGVKISDVTYQDINGTSATEVAINFDCSPSNPCTGIHLED 360

Query: 361 IQLTYNNQIPKASCENARGTASGPLQPRSCL 392
           I L YN+QIP+ASCENA+GTASGPLQPRSCL
Sbjct: 361 INLNYNDQIPQASCENAQGTASGPLQPRSCL 391

BLAST of CSPI05G14670 vs. NCBI nr
Match: KAA0062404.1 (polygalacturonase-like [Cucumis melo var. makuwa] >TYK26577.1 polygalacturonase-like [Cucumis melo var. makuwa])

HSP 1 Score: 707.6 bits (1825), Expect = 6.0e-200
Identity = 343/391 (87.72%), Postives = 370/391 (94.63%), Query Frame = 0

Query: 1   MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA 60
           MPLVGNL LL LLFLFLNHGLAAGSTFNIVSLGA ADG TDAS AFQEAWANACGSSEPA
Sbjct: 1   MPLVGNLHLLFLLFLFLNHGLAAGSTFNIVSLGAKADGMTDASPAFQEAWANACGSSEPA 60

Query: 61  TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY 120
           TIYVPNGAFY+ SGNF+GPCKNNAIT++INGTL+ASSNIQVLAQSQAWIAFR INGLSIY
Sbjct: 61  TIYVPNGAFYVHSGNFTGPCKNNAITVLINGTLMASSNIQVLAQSQAWIAFRHINGLSIY 120

Query: 121 GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ 180
           GGVIDGQG+GLWNCKHSG+SCPDGAT+LEI  +QNVNVNGLSS+NSQMFNIVVYGC+NVQ
Sbjct: 121 GGVIDGQGMGLWNCKHSGQSCPDGATSLEIFDSQNVNVNGLSSVNSQMFNIVVYGCQNVQ 180

Query: 181 IQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH 240
           IQGVNVSSA +SPNTDGIHVQQSLNVNIS+++IGTGDDCISIGPGTTNLWMENI CGPGH
Sbjct: 181 IQGVNVSSASDSPNTDGIHVQQSLNVNISNSNIGTGDDCISIGPGTTNLWMENINCGPGH 240

Query: 241 GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVN 300
           GISIGSLG+Q EEAGV+NVTVTTATF GTQNGVRIKSWGRPS+ FATKILFQHI FYNV+
Sbjct: 241 GISIGSLGKQAEEAGVQNVTVTTATFRGTQNGVRIKSWGRPSDGFATKILFQHILFYNVS 300

Query: 301 NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED 360
           NPI IDQNYCP+ Q CPG+ASGVK+SDVTYQD+NGTSA+EVAINFDCSP+NPCTGI LED
Sbjct: 301 NPIFIDQNYCPHQQSCPGRASGVKISDVTYQDINGTSATEVAINFDCSPSNPCTGIHLED 360

Query: 361 IQLTYNNQIPKASCENARGTASGPLQPRSCL 392
           I L YNNQIP+ASCENA+GTASGPLQPRSCL
Sbjct: 361 INLNYNNQIPQASCENAQGTASGPLQPRSCL 391

BLAST of CSPI05G14670 vs. NCBI nr
Match: XP_038887074.1 (polygalacturonase-like [Benincasa hispida])

HSP 1 Score: 638.3 bits (1645), Expect = 4.5e-179
Identity = 304/392 (77.55%), Postives = 356/392 (90.82%), Query Frame = 0

Query: 1   MPLVGNLP-LLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEP 60
           MP++G L  LLSLL LF  H LA G TF+IVSLGA  +GKTD+S AFQ AW NAC SS P
Sbjct: 1   MPVLGTLQLLLSLLLLFFTHDLATGLTFDIVSLGAKPNGKTDSSPAFQTAWTNACSSSVP 60

Query: 61  ATIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSI 120
           ATIYVPNG FY+QSGNF+GPCKNNAITI+I+GTL+ASS+IQVLAQ+++WI+F+ INGLSI
Sbjct: 61  ATIYVPNGVFYVQSGNFNGPCKNNAITILIDGTLMASSDIQVLAQTESWISFKDINGLSI 120

Query: 121 YGGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENV 180
            GGV+DGQGIGLWNCKHSG+SCPDGATNL IS+AQNV V+GLSS+NSQMFNIVV+GC+NV
Sbjct: 121 SGGVLDGQGIGLWNCKHSGQSCPDGATNLLISNAQNVKVSGLSSVNSQMFNIVVHGCQNV 180

Query: 181 QIQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPG 240
            +QG+NVS AG+SPNTDGIHV+QSLNV I ++SIGTGDDCISIGPGTTNLWMENI CGPG
Sbjct: 181 LVQGMNVSDAGDSPNTDGIHVEQSLNVTILNSSIGTGDDCISIGPGTTNLWMENITCGPG 240

Query: 241 HGISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNV 300
           HG+SIGSLG++ EEAGVENVTV+TATF GT+NGVRIKSWGRPS+ FAT +LFQHI FY+V
Sbjct: 241 HGVSIGSLGKEAEEAGVENVTVSTATFRGTENGVRIKSWGRPSDGFATNVLFQHIVFYDV 300

Query: 301 NNPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLE 360
            NP++IDQNYCPN++ CPGQASGVK+SDVTYQD+NGTSA+EVAINF+CSP+NPCTG+ L+
Sbjct: 301 KNPLIIDQNYCPNHESCPGQASGVKISDVTYQDMNGTSATEVAINFNCSPSNPCTGLALD 360

Query: 361 DIQLTYNNQIPKASCENARGTASGPLQPRSCL 392
           DI+LTYNN IP+ASCENA+GTASGP+QP SCL
Sbjct: 361 DIKLTYNNGIPQASCENAQGTASGPVQPSSCL 392

BLAST of CSPI05G14670 vs. NCBI nr
Match: KAE8648418.1 (hypothetical protein Csa_008806 [Cucumis sativus])

HSP 1 Score: 607.8 bits (1566), Expect = 6.5e-170
Identity = 320/391 (81.84%), Postives = 326/391 (83.38%), Query Frame = 0

Query: 1   MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA 60
           MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA
Sbjct: 1   MPLVGNLPLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPA 60

Query: 61  TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY 120
           TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY
Sbjct: 61  TIYVPNGAFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIY 120

Query: 121 GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ 180
           GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ
Sbjct: 121 GGVIDGQGIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQ 180

Query: 181 IQGVNVSSAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH 240
           IQGVNVSSAG+SPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH
Sbjct: 181 IQGVNVSSAGDSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGH 240

Query: 241 GISIGSLGRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVN 300
           GISI                   +   G  + + +        AF   I   H R     
Sbjct: 241 GISI------------------ISPCIGKHDTLNV--------AFDLYISLFHFR----- 300

Query: 301 NPILIDQNYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED 360
                              ASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED
Sbjct: 301 -------------------ASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLED 341

Query: 361 IQLTYNNQIPKASCENARGTASGPLQPRSCL 392
           IQLTYNNQIPKASC+NARGTASGPLQPRSCL
Sbjct: 361 IQLTYNNQIPKASCKNARGTASGPLQPRSCL 341

BLAST of CSPI05G14670 vs. TAIR 10
Match: AT3G59850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 447.2 bits (1149), Expect = 1.4e-125
Identity = 209/384 (54.43%), Postives = 278/384 (72.40%), Query Frame = 0

Query: 9   LLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGA 68
           LL +L   L+   ++  T+NI+S GA  DGKTD++ AF   WA AC S +P TI VP G 
Sbjct: 5   LLIVLLFLLSVSSSSAQTYNILSYGAKPDGKTDSTKAFTVLWAKACASVKPVTILVPKGR 64

Query: 69  FYIQSGNFSG-PCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYGGVIDGQ 128
           F ++S  F G  CK  ++T  I GTL+A S+ +V+ +   WI F+ ++G+S+YGGV+D Q
Sbjct: 65  FLLRSIIFDGSKCKRKSVTFRIQGTLVAPSDYRVIGKENYWILFQHLDGISVYGGVLDAQ 124

Query: 129 GIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVS 188
           G  LW+CK SGK+CP GAT++    ++NV ++GL+S+NSQMF++ + GC NV++ GV VS
Sbjct: 125 GASLWSCKKSGKNCPSGATSIGFQSSRNVVISGLTSLNSQMFHVAINGCSNVKLDGVKVS 184

Query: 189 SAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSL 248
           + GNSPNTDGIHVQ S  V+I ++ I TGDDC+SIGPGT  LW+EN+ CGPGHGISIGSL
Sbjct: 185 ADGNSPNTDGIHVQSSSTVSILNSKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSL 244

Query: 249 GRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVNNPILIDQ 308
           G++  E GV+N+TV TATFTGT+NGVRIKSW RPSN FA  I FQH    NV NPI+IDQ
Sbjct: 245 GKESVEVGVQNITVKTATFTGTENGVRIKSWARPSNGFAKNIRFQHCVMNNVQNPIVIDQ 304

Query: 309 NYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYNN 368
           NYCP N+ CP Q SG+K+SDV + D++GTSA+EV +  DCS   PCTGI ++D++LTY N
Sbjct: 305 NYCPGNENCPNQVSGIKISDVMFFDIHGTSATEVGVKLDCSSKKPCTGIRIQDVKLTYRN 364

Query: 369 QIPKASCENARGTASGPLQPRSCL 392
           +     C +A G+ +G  +P SCL
Sbjct: 365 KPATTDCSHAGGSEAGFQRPNSCL 388

BLAST of CSPI05G14670 vs. TAIR 10
Match: AT2G43870.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 436.0 bits (1120), Expect = 3.1e-122
Identity = 209/383 (54.57%), Postives = 275/383 (71.80%), Query Frame = 0

Query: 10  LSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGAF 69
           L +LF+F      +  ++N++S GA  DGKTDA+ AF   W  AC SS P TI VP G F
Sbjct: 4   LLVLFVFFFISSCSAQSYNVLSFGAKPDGKTDATKAFMAVWQTACASSRPVTIVVPKGRF 63

Query: 70  YIQSGNFSG-PCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYGGVIDGQG 129
            ++S  F G  CK   +T  I+GTL+A ++ +V+     WI F+ ++G+++YGGV+D +G
Sbjct: 64  LLRSVTFDGSKCKPKPVTFRIDGTLVAPADYRVIGNEDYWIFFQHLDGITVYGGVLDARG 123

Query: 130 IGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSS 189
             LW+CK SGK+CP GAT +    + NV V+GL+S+NSQMF++V+ GC NV++QGV V +
Sbjct: 124 ASLWDCKKSGKNCPSGATTIGFQSSSNVVVSGLTSLNSQMFHVVINGCNNVKLQGVKVLA 183

Query: 190 AGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSLG 249
           AGNSPNTDGIHVQ S +V+I +T I TGDDC+SIGPGT  LW+EN+ CGPGHGISIGSLG
Sbjct: 184 AGNSPNTDGIHVQSSSSVSIFNTKISTGDDCVSIGPGTNGLWIENVACGPGHGISIGSLG 243

Query: 250 RQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVNNPILIDQN 309
           +   E+GV+NVTV T TFTGT NGVRIKSW RPS+ FA  I FQH    NV NPI+IDQN
Sbjct: 244 KDSVESGVQNVTVKTVTFTGTDNGVRIKSWARPSSGFAKNIRFQHCVMNNVENPIIIDQN 303

Query: 310 YCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYNNQ 369
           YCP++  CP Q SG+K+SDV + D++GTSA+EV +  DCS   PCTGI LED++LTY N+
Sbjct: 304 YCPDHD-CPRQVSGIKISDVLFVDIHGTSATEVGVKLDCSSKKPCTGIRLEDVKLTYQNK 363

Query: 370 IPKASCENARGTASGPLQPRSCL 392
              ++C +A G  +G  QP +CL
Sbjct: 364 PAASACTHAGGIEAGFFQP-NCL 384

BLAST of CSPI05G14670 vs. TAIR 10
Match: AT1G05660.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 392.9 bits (1008), Expect = 3.0e-109
Identity = 186/384 (48.44%), Postives = 265/384 (69.01%), Query Frame = 0

Query: 9   LLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGA 68
           LL  L  F++  ++A + FN+VS GA  DG TD++ AF +AW  AC S+  AT+ VP G 
Sbjct: 10  LLFTLLTFIDVSISASNVFNVVSFGAKPDGVTDSTGAFLKAWQGACVSASSATVVVPKGT 69

Query: 69  FYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYGGVIDGQG 128
           F ++   F GPCK+  IT  + GT+IA  + +    S  WI F ++N  S+ GG  D + 
Sbjct: 70  FLLKVITFGGPCKSK-ITFQVAGTVIAPEDYRTFGNSGFWILFNKVNRFSLVGGTFDARA 129

Query: 129 IGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSS 188
            G W+C+ SG++CP G  ++  + A++V ++G+ S+NSQ+ ++ + GC NV ++ V + +
Sbjct: 130 NGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVTHMTLNGCTNVVVRNVKLVA 189

Query: 189 AGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSLG 248
            GNSPNTDG HVQ S  V  + +++ TGDDC++IGPGT NL +  + CGPGHG+SIGSL 
Sbjct: 190 PGNSPNTDGFHVQHSTGVTFTGSTVQTGDDCVAIGPGTRNLLITKLACGPGHGVSIGSLA 249

Query: 249 RQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVNNPILIDQN 308
           ++++E GVENVTV+++ FTG+QNGVRIKSW RPSN F   + FQ +   NV NPI+IDQN
Sbjct: 250 KELKEDGVENVTVSSSVFTGSQNGVRIKSWARPSNGFVRTVFFQDLVMKNVENPIIIDQN 309

Query: 309 YCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYNNQ 368
           YCP ++GCP + SGVK+S VTY+++ GTSA++ A+   CS ++PCTGITL+DI+LTYN  
Sbjct: 310 YCPTHEGCPNEYSGVKISQVTYKNIQGTSATQEAMKLVCSKSSPCTGITLQDIKLTYNKG 369

Query: 369 IPKAS-CENARGTASGPLQPRSCL 392
            P  S C NA G + G +QP SCL
Sbjct: 370 TPATSFCFNAVGKSLGVIQPTSCL 392

BLAST of CSPI05G14670 vs. TAIR 10
Match: AT1G05650.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 391.3 bits (1004), Expect = 8.9e-109
Identity = 184/385 (47.79%), Postives = 264/385 (68.57%), Query Frame = 0

Query: 8   PLLSLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNG 67
           PL+  L  F++   +A   FN+VS GA  DG TD++ AF +AW  ACGS+  AT+ VP G
Sbjct: 9   PLIFTLLTFIDVSSSASIVFNVVSFGAKPDGVTDSTAAFLKAWQGACGSAASATVVVPTG 68

Query: 68  AFYIQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYGGVIDGQ 127
            F ++   F GPCK+  IT  + GT++A  + +    S +WI F ++N  S+ GG  D +
Sbjct: 69  TFLLKVITFGGPCKSK-ITFQVTGTVVAPEDYRTFGNSGSWILFNKVNRFSLVGGTFDAR 128

Query: 128 GIGLWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVS 187
           G G W+C+ SG++CP G  ++  + A++V ++G+ S+NSQ+ ++ + GC NV ++ + + 
Sbjct: 129 GSGFWSCRKSGQNCPPGVRSISFNSAKDVIISGVKSMNSQVSHMTLNGCTNVAVRNIRLV 188

Query: 188 SAGNSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSL 247
           + G+SPNTDG  VQ S  V ++ +++ TGDDC++IG GT N  +  + CGPGHG+SIGSL
Sbjct: 189 APGDSPNTDGFTVQFSTGVTLTGSTVQTGDDCVAIGQGTRNFLISKLACGPGHGVSIGSL 248

Query: 248 GRQVEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVNNPILIDQ 307
            +Q+ E GVENVTV+++ FTG+QNGVRIKSW RPS  F   + FQ++   NV NPI+IDQ
Sbjct: 249 AKQLNEDGVENVTVSSSVFTGSQNGVRIKSWARPSTGFVRNVFFQNLIMRNVQNPIIIDQ 308

Query: 308 NYCPNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYNN 367
           NYCP+NQGCP + SGVK++ VTY+++ GTSA++ A+   CS +NPCTGITL+DI+LTYN 
Sbjct: 309 NYCPSNQGCPTEHSGVKITQVTYKNIQGTSATQEAMKLVCSKSNPCTGITLQDIKLTYNK 368

Query: 368 QIPKAS-CENARGTASGPLQPRSCL 392
             P  S C NA G   G +QP SCL
Sbjct: 369 GTPATSLCFNAVGKNLGVIQPTSCL 392

BLAST of CSPI05G14670 vs. TAIR 10
Match: AT2G43880.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 387.5 bits (994), Expect = 1.3e-107
Identity = 184/381 (48.29%), Postives = 254/381 (66.67%), Query Frame = 0

Query: 11  SLLFLFLNHGLAAGSTFNIVSLGAIADGKTDASHAFQEAWANACGSSEPATIYVPNGAFY 70
           S+   FL     A  +FN+   GA  DG+ DA+ +F  AW+ ACGS   A +YVP G + 
Sbjct: 15  SINIFFLIKSSHAMPSFNVQRYGARGDGRADATKSFLTAWSLACGSRARAMVYVPRGTYL 74

Query: 71  IQSGNFSGPCKNNAITIVINGTLIASSNIQVLAQSQAWIAFRQINGLSIYGGVIDGQGIG 130
           +++  F GPCK N IT   +GTL+A +N   +  S  WI F ++N +S+YGG ID +G G
Sbjct: 75  VKNLVFWGPCK-NIITFKNDGTLVAPANYWDIGNSGYWILFAKVNRISVYGGTIDARGAG 134

Query: 131 LWNCKHSGKSCPDGATNLEISHAQNVNVNGLSSINSQMFNIVVYGCENVQIQGVNVSSAG 190
            W+C+  G  CP GA ++  S   NV ++GLSS NSQ  ++ V+   NV+I+ V + +  
Sbjct: 135 YWSCRKKGSHCPQGARSISFSWCNNVLLSGLSSFNSQNMHVTVHHSSNVRIENVRIRAPS 194

Query: 191 NSPNTDGIHVQQSLNVNISSTSIGTGDDCISIGPGTTNLWMENIKCGPGHGISIGSLGRQ 250
            SPNTDGIHVQ S  V IS  +I TGDDCI++  G+ N+W+E + CGPGHGISIGSLG  
Sbjct: 195 GSPNTDGIHVQSSSGVTISGGTIATGDDCIALSQGSRNIWIERVNCGPGHGISIGSLGDY 254

Query: 251 VEEAGVENVTVTTATFTGTQNGVRIKSWGRPSNAFATKILFQHIRFYNVNNPILIDQNYC 310
             E GV+NVTVT++ FT TQNGVRIK+W RPS  F   ++F+++   NV NP++IDQNYC
Sbjct: 255 ANEEGVQNVTVTSSVFTKTQNGVRIKTWARPSRGFVNNVVFRNLIMNNVENPVIIDQNYC 314

Query: 311 PNNQGCPGQASGVKVSDVTYQDVNGTSASEVAINFDCSPTNPCTGITLEDIQLTYNNQIP 370
           PN +GCP Q+SGVK+S VT+ ++ GTS + +A+  DCS +N CTG+ L+DI+LTY  +  
Sbjct: 315 PNGKGCPRQSSGVKISGVTFANIKGTSTTPIAMKLDCSGSNHCTGLRLQDIKLTYMRRSS 374

Query: 371 KASCENARGTASGPLQPRSCL 392
            + C NA G ASG + PR+C+
Sbjct: 375 ASYCRNAHGRASGVMVPRNCM 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P489792.6e-13760.94Polygalacturonase OS=Prunus persica OX=3760 PE=2 SV=1[more]
O228182.6e-10551.76Probable polygalacturonase At2g43860 OS=Arabidopsis thaliana OX=3702 GN=At2g4386... [more]
P353393.5e-7841.55Exopolygalacturonase OS=Zea mays OX=4577 GN=PG2C PE=2 SV=1[more]
P353386.2e-7540.48Exopolygalacturonase OS=Zea mays OX=4577 GN=PG9 PE=2 SV=1[more]
P262161.8e-7439.95Exopolygalacturonase OS=Zea mays OX=4577 GN=PG1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A0A0KN911.4e-22399.23Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G409610 PE=3 SV=1[more]
A0A5D3DS412.9e-20087.72Polygalacturonase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3... [more]
A0A1S3CCA22.9e-20087.98polygalacturonase-like OS=Cucumis melo OX=3656 GN=LOC103499335 PE=3 SV=1[more]
A0A6J1F3I55.5e-15969.97polygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111439547 PE=3 SV=1[more]
A0A6J1IEE12.7e-15869.97polygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111472451 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_004140371.13.0e-22399.23polygalacturonase [Cucumis sativus][more]
XP_008460550.16.0e-20087.98PREDICTED: polygalacturonase-like [Cucumis melo][more]
KAA0062404.16.0e-20087.72polygalacturonase-like [Cucumis melo var. makuwa] >TYK26577.1 polygalacturonase-... [more]
XP_038887074.14.5e-17977.55polygalacturonase-like [Benincasa hispida][more]
KAE8648418.16.5e-17081.84hypothetical protein Csa_008806 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
AT3G59850.11.4e-12554.43Pectin lyase-like superfamily protein [more]
AT2G43870.13.1e-12254.57Pectin lyase-like superfamily protein [more]
AT1G05660.13.0e-10948.44Pectin lyase-like superfamily protein [more]
AT1G05650.18.9e-10947.79Pectin lyase-like superfamily protein [more]
AT2G43880.11.3e-10748.29Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 226..246
e-value: 8600.0
score: 0.5
coord: 203..224
e-value: 5200.0
score: 2.1
coord: 286..307
e-value: 3100.0
score: 3.7
coord: 256..277
e-value: 76.0
score: 12.2
coord: 176..202
e-value: 11.0
score: 15.0
coord: 320..360
e-value: 2700.0
score: 4.2
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 19..389
e-value: 4.4E-110
score: 370.5
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 54..381
e-value: 3.0E-84
score: 282.9
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 233..246
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 11..390
NoneNo IPR availablePANTHERPTHR31375:SF219PUTATIVE-RELATEDcoord: 11..390
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 12..391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G14670.1CSPI05G14670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity