CSPI05G13460 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G13460
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
DescriptionDAR GTPase 3, chloroplastic
LocationChr5: 13121274 .. 13125219 (-)
RNA-Seq ExpressionCSPI05G13460
SyntenyCSPI05G13460
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCACATCATTTATAGTAAGGTGGGTCACGTACAATAGTATCTACGTAGCCTTTTATCCTCTACTCGTACTCATAGTCATGGATTTAGGATTTTATAATAGGAAATAATGAATTCAAATTCTCATTAACAACTTTATTCAATCCAAATTTTGAAAAATGGGGTTATTTGACCATTTCACCTTTAAAATAGGTTATTTCTTTTTGGCATGTTATCGTGCAATTTTCCTTTGCGGCTTCCTTACCTTATCTGCGCTTGTACGAGTAATAATCTGTTCTAAAATGCTGGAATGAGTCTTCATCTTCTTCATATGGTTTAATCATTGACGCTCACTACAACTGCAAGCAATGAGTCTTCATATTCATCCCTCAGGGTTCTTCCTCCCCAATACTCGCACAATCTACCCCCAGCTCTTTTTCTGCCGAAGCGGACCATCTCCACCATCTCTCGCCCCATCAGCCTCTGTTTCTTCGCCTCACCCCACAATTCAGGTAATACCACGAATTCAATTCTCCTTCCAAGCAGCATTTGGCTGCTACTTTTGTTTCTTTAATTTTAATCTCTTATACTGTTTACGACTATCACAATCGTTCTTGTTTCTACATCACTTGTGTTACTCTCATACCTCTCTATTAGCTTTTTATACAACTTACCTTTAGTAGTTATTGAATTTAGTTTTGAAATTTCATAATGTTTTAGATTGTTGGTGGTAAAACATCAAATTTCCTTGGAGATTCGACCCCCAAAGGTGCATCTAACAACGAACTACTTGAGGGTGATTGGGTTGATTTTGAAGCTGATCTGTATTACTGGACAAATGCATTACGTCCCGTTCAGGTATGATAAAATGCCTTCTGGTTTTGAAAATAGGGCAAGATTCTTGATGAATATGCTAAAGAATATATATATTTTCCCTCCACGTTTGGTGCTGATGTGCTGGCATCTCGGCAGTGGTATCCTGGGCATATAGCAAAAACAGAAAAAGAGCTGAAGGATCAACTAAAGCTGATGGATGTTGTGATAGAGGTTCGAGATGCTAGAATACCCATGTCGACAAGTCACCCGCAGGTTTTGCATTCAAGTTCTGATTTTGTTCATTTCATTCATTATACTCTTGACTCGCCATTTTTTTTTAATGTATGTATTATTGAAGTGTTTAGGAGATTTGACTCTTTTCAATTCTTAATTGGATTGTAACTATCGGTACCCTTACCCTTACTACATTGCCATTGACATGACGAAAAGCATGGAGGCTGTAAGTTTTTTGGCTAAGGTCTGGGTGAGGTTGGGAAAAGGAAAGTTGGTGGGTTTGGGGAAAATTAGAACAGTGGCGTTTAGTAGATAATGAGATAGAGTGAACGAGGTGCTTGGCTGGGGTATTTGGTAGGTATTAGGATGGGGTCAGTATCGAAAGCTTGTCTGCTGAGTGGAGAAAGGAAGAGACCATGGCTGTGGTTTTTGTGTGGATAGAAGAGGTTTGTTTGTTGGAATGGTAAAGTTGAAGTGAATTTTGGGGTACCAATGCCTTGAACTGGAGGTGCGGTGAGAGGCAGATTTGCTAAAGGGCTTGAAAGAGACGGAGGACTTTGTGTTATTACCATAGTCGTGACTCTAGAGCTTTTGTTGGCAATTGAAGGACGAATTGCAAGAGGAAAACTCCACACTGGAGGAAGAAACTTTTTTTGATGCTGCTAGAATACCTTGAAGCTCTAATACCCAATTACAATAAAGAGAACACAGAAAGGAATATTTAATTTTTATTTTTTGAAACGGAGACGAAAGGAATATTGATGAAAAAGAGGAAGTTCTCTGCACACGTTGTCTGAGAGTAAAAGAATTCGTTTAAAGAAATGTATATCAATGTGTCGACTACTTACTAGATACAACAAAGTAAAAAGGAAAAGAGGTCAAAACTATAAAGATGCACATAATTACCATGATAACAGGGAGTACGGTGAAAATTAAGAAAACTACAAATATGGCTTAACAACTTTTAGTGTTGCTTTAACTTGCCACATTTTAGTGTATCTACACTGATTTCTTTTACTTGCTTTAGTGCTTTATATTTCATTACTCAAAGATTATTTGACCACAATCTTTTCTTATATTTACAAATTTATTCATCATTTCCATGGTTAGATGGATGCATGGCTTGGTAATAGGAGAAGAATATTGGTATTGAATAGAGAAGATATGATATCTTCAGCAGACCGGAATGCTTGGGCAACTTATTTTACAAGACAGGGAATAAAAGTAGTGTTTTCAAATGGGCAGCATGGAATGGTATCTAAACAAGCATTATTCTCTTTCTAATCATGTGGAGTTTGACATTGTAATAGTCAATAATGTTTTCATGTTGCAACCTTAGGGCACAATGAAACTAGGGCGGTTGGCCAAGACATTAGCAGCAGATGTAAATGTCAAACGTAGAGCAAAGGGACTACTTCCTCGAGCGGTTTGTTGCATGAACTCACTCAATTCTAATGTTTAAGCTTGTTTTCAAGGTTTTTATATGTCAGAATTCTTTCTATGTCAAATCATCTGCATAGCATAAATGGTTTTTGTTCTATTTTGACACAACCAAAAGACATTTAGCCTTAAGCCTGAGCGAAGCATATTATTAGCTTTAACTGCTTAAGGCACCGTGTAGCAGCTAAAAATATTTTTCTGTGCTACAGCAATCAGTGAAAATCGTGGGGCTATCGGGCTATCTCGAGTTTATCCTTAATAGTAAATCAAAATTATTTTTAGAACTTGACTTACAACCTTAGTGATTATTGCATAGGTTCGTGCTGGAATAGTTGGATATCCTAATGTTGGAAAATCGTCTTTGATCAACCGCTTGTTGAAACGACGAATGTGTCCAGCAGCTCCAAGACCAGGTGTCACCAGAGAATTGAAGTATGATACTTTTGTTTTTTAATCTCAGTGTTAACTAAGACGTAAAAGCTGGCAATGTGGGAGAGAATTAGTTAAAAAGCCATTTTCATTCCCAGTAGGGTTCAGTGAAATCTATGATTAACCGTCTCCTTATTTTCATTCTCTCGTAAAAGGTGGGTTCGTTTTGGCAACGATCTTGAGTTGCTTGATTCTCCTGGTATCATACCAATGCGGATTAGTGACCAGACAGCTGCAATAAAGCTTGCTATATGCGATGACATTGGAGAGAGGTCCTACAATGCTGCTGATGTTGCTGCCATTCTTGTACAGATTCTGACCAAGCTCCCATCAGTAGGTACCATGGAAATACTTTGATCTCTTGAAAATTTACGGAGTACTAGTAACTCTCTTGGTGGGACTGGCATATTTCCTGCACTACTATGATAGACCCCTATCATCCACCAATATACTAGACGAGGGAAGAAGAATTAAGTAGGCACATGTGAATATTAGTGTAGGCACGTGTGAGTTGTTAGGGTAAATAGTTAGGATATGTTTTATAAATAGGTTTGAATTATTGGAGGGAAGGGCAGTTAGGTTGAGATTTGAGTCTGGAATCCGTGCACCTTCGAGAGAGAACGTAACAAAGAGATTGTAACCGAATTGAGATTTCTCAATTCGTTTCTTTTATTATTTTACTTTGACCTTGTACTCGATTTCTTTTCATTTATCAATAAAAGTTCCATTGATAGAACACAATCGTAGTGTTCCATCCTATTGGTATAAGAGCGGTCGATTTAAGGAAGAAACGATTTAATGATGGTTCAGACGCGTATGGAAGAAAAATTGGAAGCGTATACGATCACGAGATGATTGGTGTAAAGAAGTAACTAAGCTAGTTGCCGGCGATGGAGGAGAAGCCGACGTTGATTACGACAACATTGGAGAATTTGAGTGTGCAGTCAGACAAAAATCAGCAGATGATGATGTTGTTTGTGGAAACAATGGCAAAGGAACGAACGGCCACAAGAGAGAGAAAGGCCGAGCCAAGTGTGCGCAACACCAGTGA

mRNA sequence

TCACATCATTTATAGTAAGGTGGGTCACGTACAATAGTATCTACGTAGCCTTTTATCCTCTACTCGTACTCATAGTCATGGATTTAGGATTTTATAATAGGAAATAATGAATTCAAATTCTCATTAACAACTTTATTCAATCCAAATTTTGAAAAATGGGGTTATTTGACCATTTCACCTTTAAAATAGGTTATTTCTTTTTGGCATGTTATCGTGCAATTTTCCTTTGCGGCTTCCTTACCTTATCTGCGCTTGTACGAGTAATAATCTGTTCTAAAATGCTGGAATGAGTCTTCATCTTCTTCATATGGTTTAATCATTGACGCTCACTACAACTGCAAGCAATGAGTCTTCATATTCATCCCTCAGGGTTCTTCCTCCCCAATACTCGCACAATCTACCCCCAGCTCTTTTTCTGCCGAAGCGGACCATCTCCACCATCTCTCGCCCCATCAGCCTCTGTTTCTTCGCCTCACCCCACAATTCAGATTGTTGGTGGTAAAACATCAAATTTCCTTGGAGATTCGACCCCCAAAGGTGCATCTAACAACGAACTACTTGAGGGTGATTGGGTTGATTTTGAAGCTGATCTGTATTACTGGACAAATGCATTACGTCCCGTTCAGTGGTATCCTGGGCATATAGCAAAAACAGAAAAAGAGCTGAAGGATCAACTAAAGCTGATGGATGTTGTGATAGAGGTTCGAGATGCTAGAATACCCATGTCGACAAGTCACCCGCAGATGGATGCATGGCTTGGTAATAGGAGAAGAATATTGGTATTGAATAGAGAAGATATGATATCTTCAGCAGACCGGAATGCTTGGGCAACTTATTTTACAAGACAGGGAATAAAAGTAGTGTTTTCAAATGGGCAGCATGGAATGGGCACAATGAAACTAGGGCGGTTGGCCAAGACATTAGCAGCAGATGTAAATGTCAAACGTAGAGCAAAGGGACTACTTCCTCGAGCGGTTCGTGCTGGAATAGTTGGATATCCTAATGTTGGAAAATCGTCTTTGATCAACCGCTTGTTGAAACGACGAATGTGTCCAGCAGCTCCAAGACCAGGTGTCACCAGAGAATTGAAGTGGGTTCGTTTTGGCAACGATCTTGAGTTGCTTGATTCTCCTGGTATCATACCAATGCGGATTAGTGACCAGACAGCTGCAATAAAGCTTGCTATATGCGATGACATTGGAGAGAGGTCCTACAATGCTGCTGATGTTGCTGCCATTCTTGTACAGATTCTGACCAAGCTCCCATCAGGAAGGGCAGTTAGGTTGAGATTTGAGTCTGGAATCCGTGCACCTTCGAGAGAGAACGTAACAAAGAGATTACGCGTATGGAAGAAAAATTGGAAGCGTATACGATCACGAGATGATTGGTGTAAAGAAGTAACTAAGCTAGTTGCCGGCGATGGAGGAGAAGCCGACGTTGATTACGACAACATTGGAGAATTTGAGTGTGCAGTCAGACAAAAATCAGCAGATGATGATGTTGTTTGTGGAAACAATGGCAAAGGAACGAACGGCCACAAGAGAGAGAAAGGCCGAGCCAAGTGTGCGCAACACCAGTGA

Coding sequence (CDS)

ATGAGTCTTCATATTCATCCCTCAGGGTTCTTCCTCCCCAATACTCGCACAATCTACCCCCAGCTCTTTTTCTGCCGAAGCGGACCATCTCCACCATCTCTCGCCCCATCAGCCTCTGTTTCTTCGCCTCACCCCACAATTCAGATTGTTGGTGGTAAAACATCAAATTTCCTTGGAGATTCGACCCCCAAAGGTGCATCTAACAACGAACTACTTGAGGGTGATTGGGTTGATTTTGAAGCTGATCTGTATTACTGGACAAATGCATTACGTCCCGTTCAGTGGTATCCTGGGCATATAGCAAAAACAGAAAAAGAGCTGAAGGATCAACTAAAGCTGATGGATGTTGTGATAGAGGTTCGAGATGCTAGAATACCCATGTCGACAAGTCACCCGCAGATGGATGCATGGCTTGGTAATAGGAGAAGAATATTGGTATTGAATAGAGAAGATATGATATCTTCAGCAGACCGGAATGCTTGGGCAACTTATTTTACAAGACAGGGAATAAAAGTAGTGTTTTCAAATGGGCAGCATGGAATGGGCACAATGAAACTAGGGCGGTTGGCCAAGACATTAGCAGCAGATGTAAATGTCAAACGTAGAGCAAAGGGACTACTTCCTCGAGCGGTTCGTGCTGGAATAGTTGGATATCCTAATGTTGGAAAATCGTCTTTGATCAACCGCTTGTTGAAACGACGAATGTGTCCAGCAGCTCCAAGACCAGGTGTCACCAGAGAATTGAAGTGGGTTCGTTTTGGCAACGATCTTGAGTTGCTTGATTCTCCTGGTATCATACCAATGCGGATTAGTGACCAGACAGCTGCAATAAAGCTTGCTATATGCGATGACATTGGAGAGAGGTCCTACAATGCTGCTGATGTTGCTGCCATTCTTGTACAGATTCTGACCAAGCTCCCATCAGGAAGGGCAGTTAGGTTGAGATTTGAGTCTGGAATCCGTGCACCTTCGAGAGAGAACGTAACAAAGAGATTACGCGTATGGAAGAAAAATTGGAAGCGTATACGATCACGAGATGATTGGTGTAAAGAAGTAACTAAGCTAGTTGCCGGCGATGGAGGAGAAGCCGACGTTGATTACGACAACATTGGAGAATTTGAGTGTGCAGTCAGACAAAAATCAGCAGATGATGATGTTGTTTGTGGAAACAATGGCAAAGGAACGAACGGCCACAAGAGAGAGAAAGGCCGAGCCAAGTGTGCGCAACACCAGTGA

Protein sequence

MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGDSTPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHGMGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAPRPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILVQILTKLPSGRAVRLRFESGIRAPSRENVTKRLRVWKKNWKRIRSRDDWCKEVTKLVAGDGGEADVDYDNIGEFECAVRQKSADDDVVCGNNGKGTNGHKREKGRAKCAQHQ*
Homology
BLAST of CSPI05G13460 vs. ExPASy Swiss-Prot
Match: Q8H1F6 (DAR GTPase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGP3 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.4e-109
Identity = 198/277 (71.48%), Postives = 231/277 (83.39%), Query Frame = 0

Query: 36  PSASVSSPHPTIQIVGGKTSNFLGDSTPKGASNNELLEG-----DWVDFEADLYYWTNAL 95
           P+A+ S P P IQIVGGK  N   D T K  S    LE      DW++ E+D+  WT AL
Sbjct: 37  PTANSSRP-PPIQIVGGKDLNL--DVTRKDDSIGFKLEANEDEIDWMNLESDIRLWTRAL 96

Query: 96  RPVQWYPGHIAKTEKELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWLGNRRRILVLNRE 155
           RPVQWYPGHI KTEKEL++QLKLMDVVIEVRDARIP+ST+HP+MDAWLGNR+RILVLNRE
Sbjct: 97  RPVQWYPGHIMKTEKELREQLKLMDVVIEVRDARIPLSTTHPKMDAWLGNRKRILVLNRE 156

Query: 156 DMISSADRNAWATYFTRQGIKVVFSNGQHGMGTMKLGRLAKTLAADVNVKRRAKGLLPRA 215
           DMIS+ DRN WA YF +QGIKV+F+NG+ GMG MKLGRLAK+LA DVN KRR KGLLPR+
Sbjct: 157 DMISNDDRNDWARYFAKQGIKVIFTNGKLGMGAMKLGRLAKSLAGDVNGKRREKGLLPRS 216

Query: 216 VRAGIVGYPNVGKSSLINRLLKRRMCPAAPRPGVTRELKWVRFGNDLELLDSPGIIPMRI 275
           VRAGI+GYPNVGKSSLINRLLKR++C AAPRPGVTRE+KWV+ G DL+LLDSPG++PMRI
Sbjct: 217 VRAGIIGYPNVGKSSLINRLLKRKICAAAPRPGVTREMKWVKLGKDLDLLDSPGMLPMRI 276

Query: 276 SDQTAAIKLAICDDIGERSYNAADVAAILVQILTKLP 308
            DQ AAIKLAICDDIGE++Y+  DVA ILVQ+L ++P
Sbjct: 277 DDQAAAIKLAICDDIGEKAYDFTDVAGILVQMLARIP 310

BLAST of CSPI05G13460 vs. ExPASy Swiss-Prot
Match: E0TTS5 (Ribosome biogenesis GTPase A OS=Bacillus subtilis subsp. spizizenii (strain ATCC 23059 / NRRL B-14472 / W23) OX=655816 GN=rbgA PE=3 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 6.0e-44
Identity = 86/211 (40.76%), Postives = 135/211 (63.98%), Query Frame = 0

Query: 93  VQWYPGHIAKTEKELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWLGNRRRILVLNREDM 152
           +QW+PGH+AK  +E+ ++LKL+D+V E+ DARIPMS+ +P ++  L N+ RI++LN+ D 
Sbjct: 3   IQWFPGHMAKARREVTEKLKLIDIVYELVDARIPMSSRNPMIEDILKNKPRIMLLNKADK 62

Query: 153 ISSADRNAWATYFTRQGIKVVFSNGQHGMGTMKLGRLAKTLAADVNVKRRAKGLLPRAVR 212
             +A    W  +F  QGI+ +  N  +G G  ++   +K +  +   + RAKG+ PRA+R
Sbjct: 63  ADAAVTQQWKEHFENQGIRSLSINSVNGQGLNQIVPASKEILQEKFDRMRAKGVKPRAIR 122

Query: 213 AGIVGYPNVGKSSLINRLLKRRMCPAAPRPGVTRELKWVRFGNDLELLDSPGIIPMRISD 272
           A I+G PNVGKS+LINRL K+ +     RPG+T   +WV+ G +LELLD+PGI+  +  D
Sbjct: 123 ALIIGIPNVGKSTLINRLAKKNIAKTGDRPGITTSQQWVKVGKELELLDTPGILWPKFED 182

Query: 273 QTAAIKLAICDDIGERSYNAADVAAILVQIL 304
           +   ++LA+   I +   N  DVA   ++ L
Sbjct: 183 ELVGLRLAVTGAIKDSIINLQDVAVFGLRFL 213

BLAST of CSPI05G13460 vs. ExPASy Swiss-Prot
Match: O31743 (Ribosome biogenesis GTPase A OS=Bacillus subtilis (strain 168) OX=224308 GN=rbgA PE=1 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 6.0e-44
Identity = 86/211 (40.76%), Postives = 135/211 (63.98%), Query Frame = 0

Query: 93  VQWYPGHIAKTEKELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWLGNRRRILVLNREDM 152
           +QW+PGH+AK  +E+ ++LKL+D+V E+ DARIPMS+ +P ++  L N+ RI++LN+ D 
Sbjct: 3   IQWFPGHMAKARREVTEKLKLIDIVYELVDARIPMSSRNPMIEDILKNKPRIMLLNKADK 62

Query: 153 ISSADRNAWATYFTRQGIKVVFSNGQHGMGTMKLGRLAKTLAADVNVKRRAKGLLPRAVR 212
             +A    W  +F  QGI+ +  N  +G G  ++   +K +  +   + RAKG+ PRA+R
Sbjct: 63  ADAAVTQQWKEHFENQGIRSLSINSVNGQGLNQIVPASKEILQEKFDRMRAKGVKPRAIR 122

Query: 213 AGIVGYPNVGKSSLINRLLKRRMCPAAPRPGVTRELKWVRFGNDLELLDSPGIIPMRISD 272
           A I+G PNVGKS+LINRL K+ +     RPG+T   +WV+ G +LELLD+PGI+  +  D
Sbjct: 123 ALIIGIPNVGKSTLINRLAKKNIAKTGDRPGITTSQQWVKVGKELELLDTPGILWPKFED 182

Query: 273 QTAAIKLAICDDIGERSYNAADVAAILVQIL 304
           +   ++LA+   I +   N  DVA   ++ L
Sbjct: 183 ELVGLRLAVTGAIKDSIINLQDVAVFGLRFL 213

BLAST of CSPI05G13460 vs. ExPASy Swiss-Prot
Match: Q65JP4 (Ribosome biogenesis GTPase A OS=Bacillus licheniformis (strain ATCC 14580 / DSM 13 / JCM 2505 / NBRC 12200 / NCIMB 9375 / NRRL NRS-1264 / Gibson 46) OX=279010 GN=rbgA PE=3 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.0e-43
Identity = 89/211 (42.18%), Postives = 134/211 (63.51%), Query Frame = 0

Query: 93  VQWYPGHIAKTEKELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWLGNRRRILVLNREDM 152
           +QW+PGH+AK  +E+ ++LKL+D+V E+ DARIPMS+ +P ++  L N+ RI++LN+ D 
Sbjct: 3   IQWFPGHMAKARREVTEKLKLIDIVYELVDARIPMSSRNPMIEDILKNKPRIMLLNKADK 62

Query: 153 ISSADRNAWATYFTRQGIKVVFSNGQHGMGTMKLGRLAKTLAADVNVKRRAKGLLPRAVR 212
             S+   AW  +F + GI  +  N  +G G  ++   +K L  +   K +AKG+ PRA+R
Sbjct: 63  ADSSVTKAWKQHFEKDGIPTLAINSVNGQGLNQILPASKELLKEKFDKMKAKGVKPRAIR 122

Query: 213 AGIVGYPNVGKSSLINRLLKRRMCPAAPRPGVTRELKWVRFGNDLELLDSPGIIPMRISD 272
           A IVG PNVGKS+LINRL K+ +     RPGVT   +WV+ G +LELLD+PGI+  +  D
Sbjct: 123 ALIVGIPNVGKSTLINRLAKKNIAKTGDRPGVTTAQQWVKVGKELELLDTPGILWPKFED 182

Query: 273 QTAAIKLAICDDIGERSYNAADVAAILVQIL 304
           +   ++LA    I +   N  DVA   ++ L
Sbjct: 183 ELVGLRLAATGAIKDSIINLQDVAVYGLRFL 213

BLAST of CSPI05G13460 vs. ExPASy Swiss-Prot
Match: Q819W8 (Ribosome biogenesis GTPase A OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / JCM 2152 / NBRC 15305 / NCIMB 9373 / NRRL B-3711) OX=226900 GN=rbgA PE=3 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.3e-43
Identity = 85/213 (39.91%), Postives = 131/213 (61.50%), Query Frame = 0

Query: 93  VQWYPGHIAKTEKELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWLGNRRRILVLNREDM 152
           +QW+PGH+AK  +++ ++LKL+DVVIE+ DAR+P+S+ +P +D  + ++ R++VLN+ DM
Sbjct: 3   IQWFPGHMAKARRQVTEKLKLIDVVIELVDARLPLSSRNPMIDEIITHKPRLVVLNKADM 62

Query: 153 ISSADRNAWATYFTRQGIKVVFSNGQHGMGTMKLGRLAKTLAADVNVKRRAKGLLPRAVR 212
                   W  YF  +G   +  N Q G G  ++    K L  +   K  AKG+ PRA+R
Sbjct: 63  ADDRLTKQWIAYFKEKGHMAISINAQAGQGMKEIAAACKVLVKEKFDKMVAKGIRPRAIR 122

Query: 213 AGIVGYPNVGKSSLINRLLKRRMCPAAPRPGVTRELKWVRFGNDLELLDSPGIIPMRISD 272
           A IVG PNVGKS+LIN+L K+ +     RPGVT   +W++ G ++ELLD+PGI+  +  D
Sbjct: 123 ALIVGIPNVGKSTLINKLAKKNIAKTGDRPGVTTAQQWIKVGKEMELLDTPGILWPKFED 182

Query: 273 QTAAIKLAICDDIGERSYNAADVAAILVQILTK 306
           Q   ++LA    I +   N  DVA   ++ + K
Sbjct: 183 QLVGLRLATTGAIKDSILNLQDVAVYALRFMEK 215

BLAST of CSPI05G13460 vs. ExPASy TrEMBL
Match: A0A0A0KMJ7 (G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G308800 PE=4 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.2e-175
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60
           MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD
Sbjct: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60

Query: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120
           STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV
Sbjct: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120

Query: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180
           RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG
Sbjct: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180

Query: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240
           MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP
Sbjct: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240

Query: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300
           RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV
Sbjct: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300

Query: 301 QILTKLPS 309
           QILTKLPS
Sbjct: 301 QILTKLPS 308

BLAST of CSPI05G13460 vs. ExPASy TrEMBL
Match: A0A5D3CV40 (DAR GTPase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G001140 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 9.6e-170
Identity = 300/308 (97.40%), Postives = 302/308 (98.05%), Query Frame = 0

Query: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60
           MSLHIHPSGFFLPNTRTI P L FCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD
Sbjct: 1   MSLHIHPSGFFLPNTRTISPHLSFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60

Query: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120
           STPKGASN++LLEGDWVDFEADLYYWTN LRPVQWYPGHIAKTEKELKDQLKLMDVVIEV
Sbjct: 61  STPKGASNSDLLEGDWVDFEADLYYWTNTLRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120

Query: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180
           RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQ G
Sbjct: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQLG 180

Query: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240
           MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP
Sbjct: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240

Query: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300
           RPGVTRELKWVRFG DLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV
Sbjct: 241 RPGVTRELKWVRFGKDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300

Query: 301 QILTKLPS 309
           QILTKLPS
Sbjct: 301 QILTKLPS 308

BLAST of CSPI05G13460 vs. ExPASy TrEMBL
Match: A0A1S3CIW2 (DAR GTPase 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501342 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 9.6e-170
Identity = 300/308 (97.40%), Postives = 302/308 (98.05%), Query Frame = 0

Query: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60
           MSLHIHPSGFFLPNTRTI P L FCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD
Sbjct: 1   MSLHIHPSGFFLPNTRTISPHLSFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60

Query: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120
           STPKGASN++LLEGDWVDFEADLYYWTN LRPVQWYPGHIAKTEKELKDQLKLMDVVIEV
Sbjct: 61  STPKGASNSDLLEGDWVDFEADLYYWTNTLRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120

Query: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180
           RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQ G
Sbjct: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQLG 180

Query: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240
           MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP
Sbjct: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240

Query: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300
           RPGVTRELKWVRFG DLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV
Sbjct: 241 RPGVTRELKWVRFGKDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300

Query: 301 QILTKLPS 309
           QILTKLPS
Sbjct: 301 QILTKLPS 308

BLAST of CSPI05G13460 vs. ExPASy TrEMBL
Match: A0A6J1HS12 (DAR GTPase 3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466972 PE=4 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 2.4e-152
Identity = 271/308 (87.99%), Postives = 285/308 (92.53%), Query Frame = 0

Query: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60
           MSLH+  SG  LP TR I  + F+CRSGPSP SL PSAS S PHP IQIVGG+TSNFLGD
Sbjct: 1   MSLHVQLSGLCLPTTRAIPTRHFYCRSGPSPASLTPSASASLPHPAIQIVGGRTSNFLGD 60

Query: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120
            + KGA N++L+E DWVDFEADLYYWTN LRPVQWYPGHIAKTEKELKDQL+LMDVVIEV
Sbjct: 61  LSTKGAPNSDLVEKDWVDFEADLYYWTNPLRPVQWYPGHIAKTEKELKDQLRLMDVVIEV 120

Query: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180
           RDARIPMST HPQMDAWLGNRRRILVLNREDMISSADRNAWA YFTR+GIKVVFSNG+ G
Sbjct: 121 RDARIPMSTCHPQMDAWLGNRRRILVLNREDMISSADRNAWADYFTRRGIKVVFSNGKLG 180

Query: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240
           MGTMKLGRLA+TLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP
Sbjct: 181 MGTMKLGRLARTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240

Query: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300
           RPGVTREL+WVRFG DLELLDSPGIIPMRISDQTAAIKLAICDDIGE+SYNAADVAAILV
Sbjct: 241 RPGVTRELRWVRFGKDLELLDSPGIIPMRISDQTAAIKLAICDDIGEKSYNAADVAAILV 300

Query: 301 QILTKLPS 309
           QILTKLPS
Sbjct: 301 QILTKLPS 308

BLAST of CSPI05G13460 vs. ExPASy TrEMBL
Match: A0A6J1HIQ5 (DAR GTPase 3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111463946 PE=4 SV=1)

HSP 1 Score: 546.6 bits (1407), Expect = 9.0e-152
Identity = 271/308 (87.99%), Postives = 284/308 (92.21%), Query Frame = 0

Query: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60
           MSLH+  SG  LP TR I  + F+CRSGPSP SL PSAS S PH TIQIVGG+TSNFLGD
Sbjct: 1   MSLHVQLSGLCLPTTRAIPTRHFYCRSGPSPASLTPSASASLPHSTIQIVGGRTSNFLGD 60

Query: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120
              KGA N++++E DWVDFEADLYYWTN LRPVQWYPGHIAKTEKELKDQL+LMDVVIEV
Sbjct: 61  LGTKGAPNSDVVEKDWVDFEADLYYWTNPLRPVQWYPGHIAKTEKELKDQLRLMDVVIEV 120

Query: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180
           RDARIPMSTSHPQMDAW+GNRRRILVLNREDMISSADRNAWA YFTRQGIKVVFSNG+ G
Sbjct: 121 RDARIPMSTSHPQMDAWVGNRRRILVLNREDMISSADRNAWADYFTRQGIKVVFSNGKLG 180

Query: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240
           MG MKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP
Sbjct: 181 MGAMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240

Query: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300
           RPGVTREL+WVRFG DLELLDSPGIIPMRISDQTAAIKLAICDDIGE+SYNAADVAAILV
Sbjct: 241 RPGVTRELRWVRFGKDLELLDSPGIIPMRISDQTAAIKLAICDDIGEKSYNAADVAAILV 300

Query: 301 QILTKLPS 309
           QILTKLPS
Sbjct: 301 QILTKLPS 308

BLAST of CSPI05G13460 vs. NCBI nr
Match: XP_011655122.1 (DAR GTPase 3, chloroplastic [Cucumis sativus])

HSP 1 Score: 625.9 bits (1613), Expect = 2.4e-175
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60
           MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD
Sbjct: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60

Query: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120
           STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV
Sbjct: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120

Query: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180
           RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG
Sbjct: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180

Query: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240
           MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP
Sbjct: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240

Query: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300
           RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV
Sbjct: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300

Query: 301 QILTKLPS 309
           QILTKLPS
Sbjct: 301 QILTKLPS 308

BLAST of CSPI05G13460 vs. NCBI nr
Match: KAE8648331.1 (hypothetical protein Csa_023136 [Cucumis sativus])

HSP 1 Score: 625.9 bits (1613), Expect = 2.4e-175
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0

Query: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60
           MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD
Sbjct: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60

Query: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120
           STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV
Sbjct: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120

Query: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180
           RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG
Sbjct: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180

Query: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240
           MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP
Sbjct: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240

Query: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300
           RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV
Sbjct: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300

Query: 301 QILTKLPS 309
           QILTKLPS
Sbjct: 301 QILTKLPS 308

BLAST of CSPI05G13460 vs. NCBI nr
Match: XP_008463116.1 (PREDICTED: DAR GTPase 3, chloroplastic [Cucumis melo])

HSP 1 Score: 606.3 bits (1562), Expect = 2.0e-169
Identity = 300/308 (97.40%), Postives = 302/308 (98.05%), Query Frame = 0

Query: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60
           MSLHIHPSGFFLPNTRTI P L FCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD
Sbjct: 1   MSLHIHPSGFFLPNTRTISPHLSFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60

Query: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120
           STPKGASN++LLEGDWVDFEADLYYWTN LRPVQWYPGHIAKTEKELKDQLKLMDVVIEV
Sbjct: 61  STPKGASNSDLLEGDWVDFEADLYYWTNTLRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120

Query: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180
           RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQ G
Sbjct: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQLG 180

Query: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240
           MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP
Sbjct: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240

Query: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300
           RPGVTRELKWVRFG DLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV
Sbjct: 241 RPGVTRELKWVRFGKDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300

Query: 301 QILTKLPS 309
           QILTKLPS
Sbjct: 301 QILTKLPS 308

BLAST of CSPI05G13460 vs. NCBI nr
Match: KAA0066191.1 (DAR GTPase 3 [Cucumis melo var. makuwa] >TYK15230.1 DAR GTPase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 606.3 bits (1562), Expect = 2.0e-169
Identity = 300/308 (97.40%), Postives = 302/308 (98.05%), Query Frame = 0

Query: 1   MSLHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60
           MSLHIHPSGFFLPNTRTI P L FCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD
Sbjct: 1   MSLHIHPSGFFLPNTRTISPHLSFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGD 60

Query: 61  STPKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120
           STPKGASN++LLEGDWVDFEADLYYWTN LRPVQWYPGHIAKTEKELKDQLKLMDVVIEV
Sbjct: 61  STPKGASNSDLLEGDWVDFEADLYYWTNTLRPVQWYPGHIAKTEKELKDQLKLMDVVIEV 120

Query: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHG 180
           RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQ G
Sbjct: 121 RDARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQLG 180

Query: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240
           MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP
Sbjct: 181 MGTMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAP 240

Query: 241 RPGVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300
           RPGVTRELKWVRFG DLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV
Sbjct: 241 RPGVTRELKWVRFGKDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILV 300

Query: 301 QILTKLPS 309
           QILTKLPS
Sbjct: 301 QILTKLPS 308

BLAST of CSPI05G13460 vs. NCBI nr
Match: XP_038887632.1 (DAR GTPase 3, chloroplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 560.5 bits (1443), Expect = 1.2e-155
Identity = 278/306 (90.85%), Postives = 285/306 (93.14%), Query Frame = 0

Query: 3   LHIHPSGFFLPNTRTIYPQLFFCRSGPSPPSLAPSASVSSPHPTIQIVGGKTSNFLGDST 62
           LHI  SG  LP    I P LF+CRSGPSP SL+PS S+SS HPTIQIVGGKTSNF GD +
Sbjct: 4   LHIQLSGLCLPTAPAISPHLFYCRSGPSPSSLSPSTSLSSSHPTIQIVGGKTSNFFGDLS 63

Query: 63  PKGASNNELLEGDWVDFEADLYYWTNALRPVQWYPGHIAKTEKELKDQLKLMDVVIEVRD 122
           PKG SN++LLEGDWVDFEADLYYWTN LRPVQWYPGHIAKTEKELK QLKLMDVVIEVRD
Sbjct: 64  PKGTSNSDLLEGDWVDFEADLYYWTNTLRPVQWYPGHIAKTEKELKHQLKLMDVVIEVRD 123

Query: 123 ARIPMSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWATYFTRQGIKVVFSNGQHGMG 182
           ARIP+STSHPQMDAWLGNRRRILVLNREDMISSADRNAWA YFTRQGIKVVFSNGQ GMG
Sbjct: 124 ARIPLSTSHPQMDAWLGNRRRILVLNREDMISSADRNAWAAYFTRQGIKVVFSNGQLGMG 183

Query: 183 TMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAPRP 242
           TMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAPRP
Sbjct: 184 TMKLGRLAKTLAADVNVKRRAKGLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAPRP 243

Query: 243 GVTRELKWVRFGNDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILVQI 302
           GVTRELKWVRFG DLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILVQI
Sbjct: 244 GVTRELKWVRFGKDLELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILVQI 303

Query: 303 LTKLPS 309
           LTKLPS
Sbjct: 304 LTKLPS 309

BLAST of CSPI05G13460 vs. TAIR 10
Match: AT4G02790.1 (GTP-binding family protein )

HSP 1 Score: 397.9 bits (1021), Expect = 9.9e-111
Identity = 198/277 (71.48%), Postives = 231/277 (83.39%), Query Frame = 0

Query: 36  PSASVSSPHPTIQIVGGKTSNFLGDSTPKGASNNELLEG-----DWVDFEADLYYWTNAL 95
           P+A+ S P P IQIVGGK  N   D T K  S    LE      DW++ E+D+  WT AL
Sbjct: 37  PTANSSRP-PPIQIVGGKDLNL--DVTRKDDSIGFKLEANEDEIDWMNLESDIRLWTRAL 96

Query: 96  RPVQWYPGHIAKTEKELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWLGNRRRILVLNRE 155
           RPVQWYPGHI KTEKEL++QLKLMDVVIEVRDARIP+ST+HP+MDAWLGNR+RILVLNRE
Sbjct: 97  RPVQWYPGHIMKTEKELREQLKLMDVVIEVRDARIPLSTTHPKMDAWLGNRKRILVLNRE 156

Query: 156 DMISSADRNAWATYFTRQGIKVVFSNGQHGMGTMKLGRLAKTLAADVNVKRRAKGLLPRA 215
           DMIS+ DRN WA YF +QGIKV+F+NG+ GMG MKLGRLAK+LA DVN KRR KGLLPR+
Sbjct: 157 DMISNDDRNDWARYFAKQGIKVIFTNGKLGMGAMKLGRLAKSLAGDVNGKRREKGLLPRS 216

Query: 216 VRAGIVGYPNVGKSSLINRLLKRRMCPAAPRPGVTRELKWVRFGNDLELLDSPGIIPMRI 275
           VRAGI+GYPNVGKSSLINRLLKR++C AAPRPGVTRE+KWV+ G DL+LLDSPG++PMRI
Sbjct: 217 VRAGIIGYPNVGKSSLINRLLKRKICAAAPRPGVTREMKWVKLGKDLDLLDSPGMLPMRI 276

Query: 276 SDQTAAIKLAICDDIGERSYNAADVAAILVQILTKLP 308
            DQ AAIKLAICDDIGE++Y+  DVA ILVQ+L ++P
Sbjct: 277 DDQAAAIKLAICDDIGEKAYDFTDVAGILVQMLARIP 310

BLAST of CSPI05G13460 vs. TAIR 10
Match: AT2G41670.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 110.5 bits (275), Expect = 3.2e-24
Identity = 80/312 (25.64%), Postives = 141/312 (45.19%), Query Frame = 0

Query: 93  VQWYPGHIAKTEKELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWLGNRRRILVLNREDM 152
           + W+PGH+A   + ++++LKL D+VIEVRDARIP+S+++  + + +  +RRI+ LN++D+
Sbjct: 26  INWFPGHMAAATRAIRNRLKLSDLVIEVRDARIPLSSANEDLQSQMSAKRRIIALNKKDL 85

Query: 153 ISSADRNAWATYFTRQGIKVVFSNGQHGMGTMKLGRLAKTLAADVNVKRRAKGLLPRAVR 212
            +    N W  +F       +  N       MKL  L +    +V  +     ++     
Sbjct: 86  ANPNVLNKWTRHFESSKQDCIAINAHSRSSVMKLLDLVELKLKEVIAREPTLLVM----- 145

Query: 213 AGIVGYPNVGKSSLINRL-------------LKRRMCPAAPRPGVTRELKWVRFGN--DL 272
             +VG PNVGKS+LIN +             LKR      P PGVT+++   +  +   +
Sbjct: 146 --VVGVPNVGKSALINSIHQIAAARFPVQERLKR--ATVGPLPGVTQDIAGFKIAHRPSI 205

Query: 273 ELLDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILVQILTKLPSGRAVRLRFE 332
            +LDSPG++   I D    +KLA+   + +       +A   + IL    +    +   E
Sbjct: 206 YVLDSPGVLVPSIPDIETGLKLALSGSVKDSVVGEERIAQYFLAILNIRGTPLHWKYLVE 265

Query: 333 SGIRAPSRENVTK---------RLRVWKKNWKRIRSRDDWCKEVTKLVAGDGGEADVDYD 381
                P  + + K           R  + +   +    D   EV + +     E D D +
Sbjct: 266 GINEGPHADCIDKPSYNLKDLRHQRTKQPDSSALHYVGDMISEVQRSLYITLSEFDGDTE 325

BLAST of CSPI05G13460 vs. TAIR 10
Match: AT3G07050.1 (GTP-binding family protein )

HSP 1 Score: 94.4 bits (233), Expect = 2.3e-19
Identity = 73/235 (31.06%), Postives = 110/235 (46.81%), Query Frame = 0

Query: 105 KELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWL----GNRRRILVLNREDMISSADRNA 164
           KEL   ++L DV++EV DAR P+ T    M+  +     N+  +L+LN+ D++       
Sbjct: 128 KELVKVIELSDVILEVLDARDPLGTRCTDMERMVMQAGPNKHLVLLLNKIDLVPREAAEK 187

Query: 165 WATYFTRQGIKVVF--------SN-GQHGMGTMKLGRLAKT---LAADVNVK----RRAK 224
           W  Y   +   V F        SN G       K   + +T   L AD  +K        
Sbjct: 188 WLMYLREEFPAVAFKCSTQEQRSNLGWKSSKASKPSNMLQTSDCLGADTLIKLLKNYSRS 247

Query: 225 GLLPRAVRAGIVGYPNVGKSSLINRLLKRRMCPAAPRPGVTRELKWVRFGNDLELLDSPG 284
             L +++  GI+G PNVGKSSLIN L +  +      PG+TR L+ V    +++LLD PG
Sbjct: 248 HELKKSITVGIIGLPNVGKSSLINSLKRAHVVNVGATPGLTRSLQEVHLDKNVKLLDCPG 307

Query: 285 IIPMRISDQTAAIKLAICDDIGERSYNAADVAAIL-------VQILTKLPSGRAV 313
           ++ ++ S   A+I L  C  I +     + V  IL       +  L K+PS  AV
Sbjct: 308 VVMLKSSGNDASIALRNCKRIEKLDDPVSPVKEILKLCPKDMLVTLYKIPSFEAV 362

BLAST of CSPI05G13460 vs. TAIR 10
Match: AT4G10650.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 80.1 bits (196), Expect = 4.6e-15
Identity = 58/224 (25.89%), Postives = 109/224 (48.66%), Query Frame = 0

Query: 94  QWYPGHIAKTEKELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWLG-NRRRILVLNREDM 153
           +WY  H+A   + + +++ L+D V+E+RDARIP+S+ +  +  +     +RI+VLN+ ++
Sbjct: 27  RWYGPHMAAAVRAISERIPLVDFVLEIRDARIPLSSEYELLRKFSPLPSKRIIVLNKMEL 86

Query: 154 ISSADRNAWATYFTRQGIKVVFSNGQHGMGTMKLGRLAKTLAADVNVKRRAKGLLPRAVR 213
               +      YF  +   + ++   H    +K  +L   L + V    +A G       
Sbjct: 87  ADPLELKKCIDYFEERNY-LSYAVNSHNKDCVK--QLLNFLQSQVRELHKA-GHSGHTTT 146

Query: 214 AGIVGYPNVGKSSLINRLL-----------KRRMCPAAPRPGVTRELKWVRFGN--DLEL 273
             ++G PNVGKS+L N L            K +    + +PG T+++  ++ G+  ++ +
Sbjct: 147 MMLLGIPNVGKSALSNSLHHIGRISAAEKGKLKHTTVSSQPGDTKDIMSLKIGSHPNVYV 206

Query: 274 LDSPGIIPMRISDQTAAIKLAICDDIGERSYNAADVAAILVQIL 304
           LD+PGI P  + D     KLA+   I +       +A + + IL
Sbjct: 207 LDTPGIFPPNLYDAEICAKLALTGAIPDDIVGELKLARLFLTIL 246

BLAST of CSPI05G13460 vs. TAIR 10
Match: AT2G27200.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 76.3 bits (186), Expect = 6.6e-14
Identity = 55/203 (27.09%), Postives = 94/203 (46.31%), Query Frame = 0

Query: 105 KELKDQLKLMDVVIEVRDARIPMSTSHPQMDAWL----GNRRRILVLNREDMISSADRNA 164
           ++L   L+  D+++ V DAR P+    P ++A+      +++ +L++N+ D++ S  R  
Sbjct: 159 RQLWRVLERSDLIVMVVDARDPLFYRCPDLEAYAQEIDEHKKTMLLVNKADLLPSYVREK 218

Query: 165 WATYFTRQGIKVVFSNGQHGMGTMK------------------------------LGRLA 224
           WA YF+R  I  VF + +    T++                              L RL 
Sbjct: 219 WAEYFSRNNILFVFWSAKAATATLEGKPLKEQWRAPDTTQKTDNPAVKVYGRDDLLDRL- 278

Query: 225 KTLAADVNVKRRAKGLLPRA-------VRAGIVGYPNVGKSSLINRLLKRRMCPAAPRPG 267
           K  A ++   R+++G+   +       V  G VGYPNVGKSS IN L+ ++       PG
Sbjct: 279 KLEALEIVKMRKSRGVSATSTESHCEQVVVGFVGYPNVGKSSTINALVGQKRTGVTSTPG 338

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H1F61.4e-10971.48DAR GTPase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DGP3 PE=2 SV=1[more]
E0TTS56.0e-4440.76Ribosome biogenesis GTPase A OS=Bacillus subtilis subsp. spizizenii (strain ATCC... [more]
O317436.0e-4440.76Ribosome biogenesis GTPase A OS=Bacillus subtilis (strain 168) OX=224308 GN=rbgA... [more]
Q65JP41.0e-4342.18Ribosome biogenesis GTPase A OS=Bacillus licheniformis (strain ATCC 14580 / DSM ... [more]
Q819W81.3e-4339.91Ribosome biogenesis GTPase A OS=Bacillus cereus (strain ATCC 14579 / DSM 31 / JC... [more]
Match NameE-valueIdentityDescription
A0A0A0KMJ71.2e-175100.00G domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G308800 PE=4 SV=1[more]
A0A5D3CV409.6e-17097.40DAR GTPase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G001140 ... [more]
A0A1S3CIW29.6e-17097.40DAR GTPase 3, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501342 PE=4 SV=1[more]
A0A6J1HS122.4e-15287.99DAR GTPase 3, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466972 PE=4 SV=... [more]
A0A6J1HIQ59.0e-15287.99DAR GTPase 3, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111463946 PE=4 S... [more]
Match NameE-valueIdentityDescription
XP_011655122.12.4e-175100.00DAR GTPase 3, chloroplastic [Cucumis sativus][more]
KAE8648331.12.4e-175100.00hypothetical protein Csa_023136 [Cucumis sativus][more]
XP_008463116.12.0e-16997.40PREDICTED: DAR GTPase 3, chloroplastic [Cucumis melo][more]
KAA0066191.12.0e-16997.40DAR GTPase 3 [Cucumis melo var. makuwa] >TYK15230.1 DAR GTPase 3 [Cucumis melo v... [more]
XP_038887632.11.2e-15590.85DAR GTPase 3, chloroplastic isoform X2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
AT4G02790.19.9e-11171.48GTP-binding family protein [more]
AT2G41670.13.2e-2425.64P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT3G07050.12.3e-1931.06GTP-binding family protein [more]
AT4G10650.14.6e-1525.89P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G27200.16.6e-1427.09P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006073GTP binding domainPRINTSPR00326GTP1OBGcoord: 259..274
score: 27.26
coord: 213..233
score: 49.84
IPR006073GTP binding domainPFAMPF01926MMR_HSR1coord: 213..301
e-value: 1.3E-15
score: 57.4
IPR019991GTP-binding protein, ribosome biogenesisTIGRFAMTIGR03596TIGR03596coord: 93..306
e-value: 6.4E-76
score: 253.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 93..266
e-value: 6.5E-43
score: 148.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 99..280
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 387..411
NoneNo IPR availablePANTHERPTHR45782:SF5DAR GTPASE 3, CHLOROPLASTICcoord: 41..332
NoneNo IPR availablePANTHERPTHR45782MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 1coord: 41..332
NoneNo IPR availableCDDcd01856YlqFcoord: 95..265
e-value: 1.72367E-79
score: 240.893

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G13460.1CSPI05G13460.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005525 GTP binding