CSPI05G11260 (gene) Cucumber (PI 183967) v1

Overview
NameCSPI05G11260
Typegene
OrganismCucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Descriptionstaphylococcal-like nuclease CAN1
LocationChr5: 9855542 .. 9858965 (+)
RNA-Seq ExpressionCSPI05G11260
SyntenyCSPI05G11260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGTCCAAATTAATGAAACCTTAAGATTCCTATTATTGCCTAAGGCCGCAAAAAAATGGGAAACGCACTCAGGTTCCTCTGCGGCCACTTCTCCCGTCCCACCTCCGATTCCTTCCCTCCTCACGCCGCCGTATCTTCCTCCACTGCCGGCGTCTCCGCTCTCCCTCACGATCTCTTCGAATTCGAGATCACATCACAGGTAAATCTCTAGCTCCCGTTGTTGCTGTTGTTGTGGTGAGCATTATTTTAAGATTTGAATTGGATCATGTCTTGTGCCCTAGTTCAATTTGTTAATTGAACGATTTGGACTTTGATGGTGTTGACGAATTTGTTTGTTTTTTGATAGGTTCCTCCAGGGCTTAGTAAGCATGTGGTTTCATCGAAGAAGGCACAGGCGAATTGGTGAGGTTCTTTTTACGGGGTGGGGAATTTGTAGTTATGTAGTGGTGAGATTTTGATTGTTTGGTGGTCATTTGAAGGTATGCAAAATTGGCTGAGGCTTGGAAGACGGAGAAACCAACTCCACGAACTCCTGAAGAAGCTTCTAGACTTGTGATTCAGACTCTCAAAAGACACCAAAAGAAAGATGTCGAGGTACAGTAAAAAATTTAGTTTAATTATGCCATTGTTTACTCTGTTGTATGTGTTGTTTGATTGAGAATCTGGCAACCTCTTCACTTTAAATAGTTCAATTGAATGAATCAAAGTCACCCTTTCAAGTTTGGGCAGCTCTGATTATCTCATTGATTAAATTTGTTTTTCTTTCCATTTCGTGTTCAAAATTTTAGTAAAGAAAAGCTGTATGTATATACATGGAGATTTCTAGATGACTTTATAGAACACTCCAAAATCCAATGGGGTTGGATCTGATTAACTGTAGTGCAAAACAAATCACTATATACATAATAGTCAATTTCTCCAAAACCATAATTTACAAAATGGGTAAAATAAAGAACTCCCTCTACAAATTTCCGAGAAAGAAAAAAGAAAAAAGGAAAACAATTCTAGTCTCCCATATGCACTTCAAAAGGGCATGTCAAACTCACACTTAATCAAGTTTTGTCTCATGGGCAACAGGGACTATTGACATTCTATGGTCTTCCTCTTCCTCATACTCTGGTCAAACCTTCTGCTCCGGTTCCAACTGCCGTCTCGATACCTGACGGAGTCAAGTTTGAGCTGCAGACGCTGCCAGTAAGATACTTCTATGGGAAATGACCAACCATTTTGATCATAACGTGTACGTTCTCATTGTGTTAAATTCATACTAGGTTGATGCCAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGCACATCACACCCGAGAGAGTCCTCTTGTGTTCCAAAAGAGGTTAGAAATGCAGCCCTTCAAAGATCACGAGCTAGAAACGCAAAGAACTATGCAAAGGCCGATGCTCTTCACAAGACAATCACGGATGCTGGTTACAGGTATATGATCTCTATCTCTACTATATATAATTGGAATTTATAAAGAGATTGCTGAGATTCAATGCCTGTTTCTTCTTCTTCGAAAGGGTAATTACTTTTCAGAATGAGGAGGTTCTTGCAAAAAAGTACAGAATTCGTCTCAGGTAATTAAAAATGTTAAATTAGCTCTATTTGCATTAGATGCTTTAGACCCTATATTTTGATCATCGTTTGTCCTTTCTGACTCATAAACTCTCAGTGCGTTTATTTTAGTTTATGTAAATTGTAAATGTTGTCTCCTTTGATCTAACCGTTCTTTTGAATCTGGAGTTTTCGTTTGGTTTTTTGTACACTCAAATTTTGAGTTTGACTAATACTACCATTTTATTTTTGAAATTTGGATTTTTGTTTTTGTTTCGTATCTAGGTTTCAACATTTATATCTTTAACTTTAATTTTCATTTCAAACCCACTTCTATTCATTAATTAAGATGTTTAAAAATATCTATTAATTAACGTTATAAATTTTTTTATTAATTAATGTTAGAAAATTTTAATTTGACAAAATATTTACTAACTTATTGCAAAATTTCAGATTCTATCACATTCATAGACAGTTTATCGATTTCTATCCTTGATAGAATCCAAAATTTTGTTATATTTTTTAAATATTTTAGTTTATTTTACTATATTTGAAAATGCTTCCTAATCTTAAATACGAAACTTAATTAATAATAGTTTTTTAGATAAATTATTACTCATTAACACTAAAGATTAAAAAAAAAAAAAGAGTACTGATTCGAAAATCAAAATTGGAAGTGTAAATCTTGCCACCTAAGACCAAATTGAAACAATTTCAAATTTCAAAATTAAAATTATAACATATTGAAATCTGAAGCCTAAATTGAACCAACTCTTAAGATAATAACTAACTATATATATGTTTTTCCCCATAATTTGTTTCCTCATTTTTTTAATCACAATATTTATATATACACCAAACTTTGTAAAATATTATTACAACTCTATATTAAACAATTATCACAGTAAATAGTTGAGATCGTGAATTTATTCCATAAAAAAATTACAAATTGTATTGCCCCTTATAGTTGATATGATGCAGAGGAATAGACGCACCAGAAAGTGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAACTGAAAAGGCTTGTTGAAGGCAAGTGTTTGAGAGTACATGTGTATGGTGAAGATCGGTACAATCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGTAAAACACAAATTTACACAAATTTTTTTTTTGTTCTTCGCTATGATATAGGCTTCATGTTCATGTTATTGGTTTAGACAAATACAAGTTTTTGGTTCTTTACAGGAAGCAATGTTGAAAAAGGGGTTTGCTTGGCATTACACAGCATATGACAAACGTCCAGAGCTGTCAAAGGCAAGGGCTAAATTTACCATTTAACCCATCTTTAATTAATTTAGTGTTTGATTGCATACTTTTAACCCTTATTTCAAAATTAAGGTTCAAAATCACATGCAAAATTTCATTAATCATAACATAGGAAAAGCAGAGCGACACAGATTTCTCAACAAGAGCCTTTTTGGCTAAGCCTTACAAATTGTTTAATTATTGATAACAGTGGGAAAACGAGGCTCGAGCTAAAAGGGTTGGGTTGTGGGCTTCTTCAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAGGGGAAATAATAATGGCTGCTTGTTGCTATTGCTGTTTCAATACACAACACTTATAATTTATATATGAAGGACGTTCATCCATTTCATGTCTACCAACGACAAGAAGCAGAAGAGGGAACCCTAAAATCAACCCAAAAAGGTCTCTTCTGTCTTTAAAGTATTTATTAATCAACATCTTCAACATTGCTGGTAATTTGTAAATTTGTAACTGAAGAGAAATGAAAAGGAACACATTGTTA

mRNA sequence

AGTCCAAATTAATGAAACCTTAAGATTCCTATTATTGCCTAAGGCCGCAAAAAAATGGGAAACGCACTCAGGTTCCTCTGCGGCCACTTCTCCCGTCCCACCTCCGATTCCTTCCCTCCTCACGCCGCCGTATCTTCCTCCACTGCCGGCGTCTCCGCTCTCCCTCACGATCTCTTCGAATTCGAGATCACATCACAGGTTCCTCCAGGGCTTAGTAAGCATGTGGTTTCATCGAAGAAGGCACAGGCGAATTGGTATGCAAAATTGGCTGAGGCTTGGAAGACGGAGAAACCAACTCCACGAACTCCTGAAGAAGCTTCTAGACTTGTGATTCAGACTCTCAAAAGACACCAAAAGAAAGATGTCGAGGGACTATTGACATTCTATGGTCTTCCTCTTCCTCATACTCTGGTCAAACCTTCTGCTCCGGTTCCAACTGCCGTCTCGATACCTGACGGAGTCAAGTTTGAGCTGCAGACGCTGCCAGTTGATGCCAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGCACATCACACCCGAGAGAGTCCTCTTGTGTTCCAAAAGAGGTTAGAAATGCAGCCCTTCAAAGATCACGAGCTAGAAACGCAAAGAACTATGCAAAGGCCGATGCTCTTCACAAGACAATCACGGATGCTGGTTACAGGGTAATTACTTTTCAGAATGAGGAGGTTCTTGCAAAAAAGTACAGAATTCGTCTCAGAGGAATAGACGCACCAGAAAGTGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAACTGAAAAGGCTTGTTGAAGGCAAGTGTTTGAGAGTACATGTGTATGGTGAAGATCGGTACAATCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGAAGCAATGTTGAAAAAGGGGTTTGCTTGGCATTACACAGCATATGACAAACGTCCAGAGCTGTCAAAGTGGGAAAACGAGGCTCGAGCTAAAAGGGTTGGGTTGTGGGCTTCTTCAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAGGGGAAATAATAATGGCTGCTTGTTGCTATTGCTGTTTCAATACACAACACTTATAATTTATATATGAAGGACGTTCATCCATTTCATGTCTACCAACGACAAGAAGCAGAAGAGGGAACCCTAAAATCAACCCAAAAAGGTCTCTTCTGTCTTTAAAGTATTTATTAATCAACATCTTCAACATTGCTGGTAATTTGTAAATTTGTAACTGAAGAGAAATGAAAAGGAACACATTGTTA

Coding sequence (CDS)

ATGGGAAACGCACTCAGGTTCCTCTGCGGCCACTTCTCCCGTCCCACCTCCGATTCCTTCCCTCCTCACGCCGCCGTATCTTCCTCCACTGCCGGCGTCTCCGCTCTCCCTCACGATCTCTTCGAATTCGAGATCACATCACAGGTTCCTCCAGGGCTTAGTAAGCATGTGGTTTCATCGAAGAAGGCACAGGCGAATTGGTATGCAAAATTGGCTGAGGCTTGGAAGACGGAGAAACCAACTCCACGAACTCCTGAAGAAGCTTCTAGACTTGTGATTCAGACTCTCAAAAGACACCAAAAGAAAGATGTCGAGGGACTATTGACATTCTATGGTCTTCCTCTTCCTCATACTCTGGTCAAACCTTCTGCTCCGGTTCCAACTGCCGTCTCGATACCTGACGGAGTCAAGTTTGAGCTGCAGACGCTGCCAGTTGATGCCAAAGCTGTAGCAGATGGTGATACAGTGACAGTGTATGTGAGCACATCACACCCGAGAGAGTCCTCTTGTGTTCCAAAAGAGGTTAGAAATGCAGCCCTTCAAAGATCACGAGCTAGAAACGCAAAGAACTATGCAAAGGCCGATGCTCTTCACAAGACAATCACGGATGCTGGTTACAGGGTAATTACTTTTCAGAATGAGGAGGTTCTTGCAAAAAAGTACAGAATTCGTCTCAGAGGAATAGACGCACCAGAAAGTGCAATGCCTTATGGGAAAGAGGCAAAAGAAGAACTGAAAAGGCTTGTTGAAGGCAAGTGTTTGAGAGTACATGTGTATGGTGAAGATCGGTACAATCGATGTGTGGGTGACTTGTATTGTAATGGAAAGTTTATTCAGGAAGCAATGTTGAAAAAGGGGTTTGCTTGGCATTACACAGCATATGACAAACGTCCAGAGCTGTCAAAGTGGGAAAACGAGGCTCGAGCTAAAAGGGTTGGGTTGTGGGCTTCTTCAAACCCAGAGCAGCCATGGGAATGGAGAAAGGGAAAGCGTGAGGGGAAATAA

Protein sequence

MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPELSKWENEARAKRVGLWASSNPEQPWEWRKGKREGK*
Homology
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BLAST of CSPI05G11260 vs. ExPASy Swiss-Prot
Match: Q39635 (Uncharacterized 38.1 kDa protein OS=Capnoides sempervirens OX=3464 PE=2 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 4.6e-127
Identity = 231/341 (67.74%), Postives = 272/341 (79.77%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTSDSF--PPHA-AVSSSTAGVSALPHDLFEFEITSQVPPGLSKHV 60
           MGNALRFL GH  +PT +    PPH   VS++T GVSAL HDLF FE TSQ+P GL+K+V
Sbjct: 1   MGNALRFLYGHCCKPTVEDHYQPPHGYGVSTATVGVSALAHDLFHFENTSQIPEGLTKYV 60

Query: 61  VSSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPH 120
           VSSKKAQ NWY KL++AW+  KP P+T E+A+RL+I TLKRHQK DV+GLL FYGLPL +
Sbjct: 61  VSSKKAQTNWYKKLSQAWREAKPPPQTAEQATRLIILTLKRHQKADVKGLLRFYGLPLSN 120

Query: 121 TLVKPSAPVPTAVSIP---DGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKE 180
               PS    T V+ P    GVKFEL TLPVD KAVADGDTVTVYV+T  PRE+S +PK 
Sbjct: 121 ---NPSTEATTVVAPPQADQGVKFELHTLPVDVKAVADGDTVTVYVNTEDPREASNLPKS 180

Query: 181 VRNAALQRSRARNAKNYAKADALHKTITDAGYRVITF-QNEEVLAKKYRIRLRGIDAPES 240
           V+ AA +R++AR  ++Y KADAL K I DAGYRV++   NE++LA+KYRIRLRGID+PES
Sbjct: 181 VKVAAQERAKARAVRDYVKADALQKNIVDAGYRVLSGPNNEDILARKYRIRLRGIDSPES 240

Query: 241 AMPYGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTA 300
           +MP+GKEAKEEL +LV GKCL VH+Y EDRY R VGD+YCNG+FIQE MLKKG AWHYTA
Sbjct: 241 SMPFGKEAKEELIKLVVGKCLTVHIYEEDRYGRSVGDIYCNGQFIQEKMLKKGLAWHYTA 300

Query: 301 YDKRPELSKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           YDKRP+LSKWE +ARA RVGLWASSNPE+PWEWRK KR GK
Sbjct: 301 YDKRPQLSKWEEKARAARVGLWASSNPEKPWEWRKNKRNGK 338

[Close]
BLAST of CSPI05G11260 vs. ExPASy Swiss-Prot
Match: F4IH31 (Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana OX=3702 GN=CAN2 PE=1 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 8.7e-126
Identity = 227/333 (68.17%), Postives = 266/333 (79.88%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTS--DSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVV 60
           MGNAL FL G   +PT+  DS  PH  VS++T GVSAL HDLF FEITSQVP GL ++V 
Sbjct: 1   MGNALTFLYGKCCKPTTTDDSLGPH-GVSAATVGVSALAHDLFNFEITSQVPEGLGRYVQ 60

Query: 61  SSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHT 120
           SS+KAQANWY K+ EAWK  KP P+T EEASRLV   LKR+QK DVEGLL+FYGLPL HT
Sbjct: 61  SSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSHT 120

Query: 121 LVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNA 180
           LV+ +   P  VS+P+G+ FE QTLPVD KAVADGDT+TVYVSTS P  SS VP+EV  A
Sbjct: 121 LVEVTVEAP--VSLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNLA 180

Query: 181 ALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYG 240
           A+QR++AR  +NY KAD LH+ I D+GYRV+  +NEEVLA+K+RIRLRGIDAPES MP+G
Sbjct: 181 AVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLRGIDAPESQMPFG 240

Query: 241 KEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRP 300
           KEA+E L ++V  K L+V VYGED+Y RCVGDLYCNG F+QEAMLKKG AWHY AYDKRP
Sbjct: 241 KEAQEGLLKIVGRKSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLKKGLAWHYLAYDKRP 300

Query: 301 ELSKWENEARAKRVGLWASSNPEQPWEWRKGKR 332
            L+KWE EAR KR+GLWASSNPE+PW+WRK  R
Sbjct: 301 VLAKWEKEARQKRIGLWASSNPEKPWDWRKNNR 330

[Close]
BLAST of CSPI05G11260 vs. ExPASy Swiss-Prot
Match: F4IZC5 (Staphylococcal-like nuclease CAN1 OS=Arabidopsis thaliana OX=3702 GN=CAN1 PE=1 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.3e-124
Identity = 227/333 (68.17%), Postives = 261/333 (78.38%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS 60
           MGNA+R L     R   +S      VS+S+ GVSAL  DL  FE TSQVP  L  +VVSS
Sbjct: 1   MGNAIRLL-----RKCLNSH----GVSASSGGVSALSRDLLNFETTSQVPEKLGSYVVSS 60

Query: 61  KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120
           +KAQANWY K+ EAWK  KP P+TPEEASRLVI  LK HQK DVEGLL+FYGLP PH LV
Sbjct: 61  QKAQANWYRKILEAWKQAKPRPKTPEEASRLVIAALKNHQKADVEGLLSFYGLPSPHNLV 120

Query: 121 KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL 180
           +   P    VS+P GV+FEL TLPVD K+VADGDTVTVYVS+  P  SS +PK+V  AA+
Sbjct: 121 E--VPTEAPVSLPKGVRFELNTLPVDTKSVADGDTVTVYVSSKDPLVSSSLPKDVSLAAV 180

Query: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240
           +R++AR  KNY +ADALHKTI  +GYR+I+FQNEEVLAKK+RIRL GID+PES MPYGKE
Sbjct: 181 KRAKAREKKNYTEADALHKTIIASGYRMISFQNEEVLAKKFRIRLSGIDSPESKMPYGKE 240

Query: 241 AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300
           A +EL ++VEGKCL+V VY EDRY RCVGD+YCNGKF+QE MLKKG AWHY AYDKR EL
Sbjct: 241 AHDELLKMVEGKCLKVLVYTEDRYGRCVGDIYCNGKFVQEVMLKKGLAWHYVAYDKRAEL 300

Query: 301 SKWENEARAKRVGLWASSNPEQPWEWRKGKREG 334
           +KWENEAR KRVGLWASSNPE+PWEWRK KR G
Sbjct: 301 AKWENEARQKRVGLWASSNPEKPWEWRKNKRGG 322

[Close]
BLAST of CSPI05G11260 vs. ExPASy Swiss-Prot
Match: Q942N7 (Probable staphylococcal-like nuclease CAN1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0166100 PE=2 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.0e-118
Identity = 216/335 (64.48%), Postives = 256/335 (76.42%), Query Frame = 0 

Query: 1   MGNAL-RFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVS 60
           MGN++ RFLCG  S   S  + PH A       V+AL  D+ +FE TSQVP GLS+HVVS
Sbjct: 1   MGNSIYRFLCGLCS--PSPEYQPHGA----HPAVAALGRDIQQFEATSQVPDGLSRHVVS 60

Query: 61  SKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTL 120
           SKKAQANWY KL   WK  +PTPRTPEEA+RLV+ TLK HQK DVEG L FYGLP+P+  
Sbjct: 61  SKKAQANWYKKLIVTWKKARPTPRTPEEAARLVVTTLKNHQKADVEGFLVFYGLPIPNAA 120

Query: 121 VKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAA 180
               AP    V  P G KFEL TLPVDAKAVADGDT+TVY+ T+ PRES  VP+E++ AA
Sbjct: 121 ASTPAPHTAHVPKPQGCKFELHTLPVDAKAVADGDTITVYIDTADPRESGNVPREIQKAA 180

Query: 181 LQRSRARNAKNYAKADALHKTITDAGYR-VITFQNEEVLAKKYRIRLRGIDAPESAMPYG 240
            +R+RAR A++Y KAD L K I DAGYR V   + EEVLAKKYRIRLRGIDAPESAMPYG
Sbjct: 181 AERTRARAARDYQKADGLQKMIADAGYRQVPNARGEEVLAKKYRIRLRGIDAPESAMPYG 240

Query: 241 KEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRP 300
           KEAKE L ++V+GK L+V+VY EDRY RCVGD+YC+G F+QE MLKKG AWHYTAYD+RP
Sbjct: 241 KEAKEALLKMVQGKSLKVYVYDEDRYGRCVGDIYCDGVFVQEQMLKKGCAWHYTAYDQRP 300

Query: 301 ELSKWENEARAKRVGLWASSNPEQPWEWRKGKREG 334
           EL+KWE +A++ R GLWA+S P++PWEWR+ KR G
Sbjct: 301 ELAKWEKQAQSGRKGLWAASRPQKPWEWRRDKRNG 329

[Close]
BLAST of CSPI05G11260 vs. ExPASy Swiss-Prot
Match: Q2RBJ2 (Probable staphylococcal-like nuclease CAN2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0109500 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.1e-91
Identity = 164/313 (52.40%), Postives = 219/313 (69.97%), Query Frame = 0 

Query: 22  PHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSSKKAQANWYAKLAEAWKTEKPT 81
           PH  V+ ST GV+AL HDL  FE TS VP GLS+HVVSS+KAQ  WY KL EA+K   P 
Sbjct: 52  PH-GVTPSTVGVAALAHDLLNFESTSMVPDGLSQHVVSSRKAQVKWYQKLLEAYKNTTPP 111

Query: 82  PRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLVKPSAPVPTAVSIPDGVKFELQ 141
           P+TP  A++L+ + L   Q+ D+EG+L FY LP+P      S   P+   +P+GV+F L 
Sbjct: 112 PKTPANAAQLIARALNMIQRADLEGILEFYNLPIPSLPTASSNYQPSL--LPEGVQFVLN 171

Query: 142 TLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAALQRSRARNAKNYAKADALHKTI 201
           TLPV  K + DGD  T YV T+ PRES+ VP EV    + R++AR  ++Y  ADAL  ++
Sbjct: 172 TLPVYDKCIGDGDGFTAYVPTTDPRESANVPLEVHELVIARTQARKCRDYQSADALLSSL 231

Query: 202 TDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKEAKEELKRLVEGKCLRVHVYGE 261
            +AGY++I+  ++EVLA+KYRIR+RGIDAPE  MPYGKE++  L +L+ GK ++++VY  
Sbjct: 232 DEAGYKIISCSDDEVLARKYRIRMRGIDAPELKMPYGKESRTALVKLIGGKSVKIYVYDL 291

Query: 262 DRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPELSKWENEARAKRVGLWASSNPE 321
           D++ R VGD+YCN  FIQE MLK G AWH+  YDKRPE ++WE EARA   GLWAS NPE
Sbjct: 292 DQFGRYVGDIYCNNLFIQEQMLKNGHAWHFKTYDKRPEFARWEREARAANRGLWASGNPE 351

Query: 322 QPWEWRKGKREGK 335
           +PW+WR+ +R  +
Sbjct: 352 KPWDWRRDQRNAR 361

[Close]
BLAST of CSPI05G11260 vs. ExPASy TrEMBL
Match: B6CAZ6 (Calcium-dependent nuclease OS=Cucumis sativus OX=3659 GN=Csa_5G218750 PE=2 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.4e-190
Identity = 332/334 (99.40%), Postives = 333/334 (99.70%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS 60
           MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS
Sbjct: 1   MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS 60

Query: 61  KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120
           KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV
Sbjct: 61  KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120

Query: 121 KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL 180
           KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL
Sbjct: 121 KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL 180

Query: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240
           QRSRARNAKN+AKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE
Sbjct: 181 QRSRARNAKNFAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240

Query: 241 AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300
           AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL
Sbjct: 241 AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300

Query: 301 SKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           SKWENEARAKR GLWASSNPEQPWEWRKGKREGK
Sbjct: 301 SKWENEARAKRAGLWASSNPEQPWEWRKGKREGK 334

[Close]
BLAST of CSPI05G11260 vs. ExPASy TrEMBL
Match: A0A5D3DQ96 (Staphylococcal-like nuclease CAN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold436G00570 PE=4 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.8e-182
Identity = 319/334 (95.51%), Postives = 324/334 (97.01%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS 60
           MGNALRFLCGHF  PTSDS PPHAA+SSSTAGVSAL HDLFEFEITSQVPPGL KHVVSS
Sbjct: 1   MGNALRFLCGHFCHPTSDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKHVVSS 60

Query: 61  KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120
           KKAQANWY KLAEAWK+EKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV
Sbjct: 61  KKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120

Query: 121 KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL 180
           K SAP PTA SIPDGVKFELQTLPVDAKAVADGDTVTV+VSTSHPRESSCVPKEVRNAAL
Sbjct: 121 KSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVRNAAL 180

Query: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240
           QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE
Sbjct: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240

Query: 241 AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300
           AKEELKRLVEGKCLRV VYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL
Sbjct: 241 AKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300

Query: 301 SKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           SKWENEARAKRVGLWASSNP++PWEWRKGKREGK
Sbjct: 301 SKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

[Close]
BLAST of CSPI05G11260 vs. ExPASy TrEMBL
Match: A0A1S3C4Y1 (staphylococcal-like nuclease CAN1 OS=Cucumis melo OX=3656 GN=LOC103496714 PE=4 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.8e-182
Identity = 319/334 (95.51%), Postives = 324/334 (97.01%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS 60
           MGNALRFLCGHF  PTSDS PPHAA+SSSTAGVSAL HDLFEFEITSQVPPGL KHVVSS
Sbjct: 1   MGNALRFLCGHFCHPTSDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKHVVSS 60

Query: 61  KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120
           KKAQANWY KLAEAWK+EKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV
Sbjct: 61  KKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120

Query: 121 KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL 180
           K SAP PTA SIPDGVKFELQTLPVDAKAVADGDTVTV+VSTSHPRESSCVPKEVRNAAL
Sbjct: 121 KSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVRNAAL 180

Query: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240
           QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE
Sbjct: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240

Query: 241 AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300
           AKEELKRLVEGKCLRV VYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL
Sbjct: 241 AKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300

Query: 301 SKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           SKWENEARAKRVGLWASSNP++PWEWRKGKREGK
Sbjct: 301 SKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

[Close]
BLAST of CSPI05G11260 vs. ExPASy TrEMBL
Match: A0A6J1FPN5 (staphylococcal-like nuclease CAN2 OS=Cucurbita moschata OX=3662 GN=LOC111447091 PE=4 SV=1)

HSP 1 Score: 591.3 bits (1523), Expect = 2.6e-165
Identity = 290/338 (85.80%), Postives = 309/338 (91.42%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPT----SDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKH 60
           MGN LRFLCGHF  P     +DS  PH  VS +T GVSAL HDLF+FEITSQVP GLSKH
Sbjct: 1   MGNVLRFLCGHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60

Query: 61  VVSSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120
           VVSSKKAQANWY KLAEAW++EKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120

Query: 121 HTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVR 180
           HTLV+ SAP P   S+PDGVKFELQTLPVDAKAVADGDTVTVYVST+HPRESSC+P EVR
Sbjct: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180

Query: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRV VYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RPELSKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           R EL+KWENEARAKR+GLWAS NPEQPWEWRKGKREGK
Sbjct: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 337

[Close]
BLAST of CSPI05G11260 vs. ExPASy TrEMBL
Match: A0A6J1K113 (staphylococcal-like nuclease CAN2 OS=Cucurbita maxima OX=3661 GN=LOC111491421 PE=4 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 3.7e-164
Identity = 288/338 (85.21%), Postives = 308/338 (91.12%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPT----SDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKH 60
           MGNALRFLC HF  P     +DS  PH  VS +T GVSAL HDLF+FEITSQVP GLSKH
Sbjct: 1   MGNALRFLCSHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60

Query: 61  VVSSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120
           VVSSKKAQ NWY KLAEAW++EKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120

Query: 121 HTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVR 180
           HTLV+ SAP P   S+PDGVKFELQTLPVDAKAVADGDTVTVYVST+HPRESSC+P EVR
Sbjct: 121 HTLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180

Query: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRV VYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RPELSKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           R EL+KWENEARA+R+GLWAS NPEQPWEWRKGKREGK
Sbjct: 301 RAELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 337

[Close]
BLAST of CSPI05G11260 vs. NCBI nr
Match: NP_001275526.1 (Uncharacterized 38.1 kDa protein-like [Cucumis sativus] >ABX56707.1 calcium-dependent nuclease [Cucumis sativus] >ACS71330.1 calcium-dependent nuclease [Cucumis sativus] >KGN50709.1 hypothetical protein Csa_005779 [Cucumis sativus])

HSP 1 Score: 675.2 bits (1741), Expect = 2.8e-190
Identity = 332/334 (99.40%), Postives = 333/334 (99.70%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS 60
           MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS
Sbjct: 1   MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS 60

Query: 61  KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120
           KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV
Sbjct: 61  KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120

Query: 121 KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL 180
           KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL
Sbjct: 121 KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL 180

Query: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240
           QRSRARNAKN+AKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE
Sbjct: 181 QRSRARNAKNFAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240

Query: 241 AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300
           AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL
Sbjct: 241 AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300

Query: 301 SKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           SKWENEARAKR GLWASSNPEQPWEWRKGKREGK
Sbjct: 301 SKWENEARAKRAGLWASSNPEQPWEWRKGKREGK 334

[Close]
BLAST of CSPI05G11260 vs. NCBI nr
Match: XP_008456902.1 (PREDICTED: staphylococcal-like nuclease CAN1 [Cucumis melo] >TYK25847.1 staphylococcal-like nuclease CAN1 [Cucumis melo var. makuwa])

HSP 1 Score: 648.3 bits (1671), Expect = 3.7e-182
Identity = 319/334 (95.51%), Postives = 324/334 (97.01%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS 60
           MGNALRFLCGHF  PTSDS PPHAA+SSSTAGVSAL HDLFEFEITSQVPPGL KHVVSS
Sbjct: 1   MGNALRFLCGHFCHPTSDSLPPHAALSSSTAGVSALAHDLFEFEITSQVPPGLGKHVVSS 60

Query: 61  KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120
           KKAQANWY KLAEAWK+EKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV
Sbjct: 61  KKAQANWYGKLAEAWKSEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120

Query: 121 KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL 180
           K SAP PTA SIPDGVKFELQTLPVDAKAVADGDTVTV+VSTSHPRESSCVPKEVRNAAL
Sbjct: 121 KSSAPAPTAASIPDGVKFELQTLPVDAKAVADGDTVTVHVSTSHPRESSCVPKEVRNAAL 180

Query: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240
           QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE
Sbjct: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240

Query: 241 AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300
           AKEELKRLVEGKCLRV VYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL
Sbjct: 241 AKEELKRLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300

Query: 301 SKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           SKWENEARAKRVGLWASSNP++PWEWRKGKREGK
Sbjct: 301 SKWENEARAKRVGLWASSNPDKPWEWRKGKREGK 334

[Close]
BLAST of CSPI05G11260 vs. NCBI nr
Match: XP_038892991.1 (staphylococcal-like nuclease CAN1 isoform X1 [Benincasa hispida])

HSP 1 Score: 595.9 bits (1535), Expect = 2.2e-166
Identity = 292/334 (87.43%), Postives = 309/334 (92.51%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS 60
           MGN L FLCGHF  P+SDS PPH  VS +TAGVSAL HDLF+FEITSQVP GLSKHVVSS
Sbjct: 1   MGNVLGFLCGHFCNPSSDSQPPH-GVSYATAGVSALAHDLFDFEITSQVPQGLSKHVVSS 60

Query: 61  KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120
           KKAQANWY KLA+AWK EKP PRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV
Sbjct: 61  KKAQANWYRKLADAWKLEKPPPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120

Query: 121 KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL 180
           + SAP PT  S+PDGVKFEL+TLPVDAKAVADGDTVTVYVST+H RESSC+P EVRNAA+
Sbjct: 121 ELSAPAPTTASLPDGVKFELETLPVDAKAVADGDTVTVYVSTAHTRESSCIPIEVRNAAI 180

Query: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240
           QRS+ARNAKN+AKADALHKTITDAGYRV+TFQNEE+LA+KYRIRLRGIDAPES MPYGKE
Sbjct: 181 QRSQARNAKNFAKADALHKTITDAGYRVLTFQNEEILARKYRIRLRGIDAPESGMPYGKE 240

Query: 241 AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300
           AK+EL +LVEGKCLRV VYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHY AYDKR EL
Sbjct: 241 AKQELTKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYKAYDKRAEL 300

Query: 301 SKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           SKWENEARAKR GLWASSNPEQPWEWRKGKREGK
Sbjct: 301 SKWENEARAKRAGLWASSNPEQPWEWRKGKREGK 333

[Close]
BLAST of CSPI05G11260 vs. NCBI nr
Match: XP_022941859.1 (staphylococcal-like nuclease CAN2 [Cucurbita moschata] >XP_023552645.1 staphylococcal-like nuclease CAN2 [Cucurbita pepo subsp. pepo] >KAG6599960.1 Staphylococcal-like nuclease CAN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030638.1 Staphylococcal-like nuclease CAN2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 591.3 bits (1523), Expect = 5.3e-165
Identity = 290/338 (85.80%), Postives = 309/338 (91.42%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPT----SDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKH 60
           MGN LRFLCGHF  P     +DS  PH  VS +T GVSAL HDLF+FEITSQVP GLSKH
Sbjct: 1   MGNVLRFLCGHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60

Query: 61  VVSSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120
           VVSSKKAQANWY KLAEAW++EKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQANWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120

Query: 121 HTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVR 180
           HTLV+ SAP P   S+PDGVKFELQTLPVDAKAVADGDTVTVYVST+HPRESSC+P EVR
Sbjct: 121 HTLVELSAPAPVPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180

Query: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRV VYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RPELSKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           R EL+KWENEARAKR+GLWAS NPEQPWEWRKGKREGK
Sbjct: 301 RAELAKWENEARAKRIGLWASRNPEQPWEWRKGKREGK 337

[Close]
BLAST of CSPI05G11260 vs. NCBI nr
Match: XP_022996106.1 (staphylococcal-like nuclease CAN2 [Cucurbita maxima] >XP_022996112.1 staphylococcal-like nuclease CAN2 [Cucurbita maxima])

HSP 1 Score: 587.4 bits (1513), Expect = 7.7e-164
Identity = 288/338 (85.21%), Postives = 308/338 (91.12%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPT----SDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKH 60
           MGNALRFLC HF  P     +DS  PH  VS +T GVSAL HDLF+FEITSQVP GLSKH
Sbjct: 1   MGNALRFLCSHFCNPNAAADADSLGPH-GVSPATVGVSALAHDLFDFEITSQVPQGLSKH 60

Query: 61  VVSSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLP 120
           VVSSKKAQ NWY KLAEAW++EKP P+TPEEASRLVIQTLKRHQKKDVEGLL FYGLPLP
Sbjct: 61  VVSSKKAQTNWYRKLAEAWRSEKPPPKTPEEASRLVIQTLKRHQKKDVEGLLAFYGLPLP 120

Query: 121 HTLVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVR 180
           HTLV+ SAP P   S+PDGVKFELQTLPVDAKAVADGDTVTVYVST+HPRESSC+P EVR
Sbjct: 121 HTLVELSAPAPLPTSLPDGVKFELQTLPVDAKAVADGDTVTVYVSTAHPRESSCIPMEVR 180

Query: 181 NAALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMP 240
           NAA+QRS+ARNAKNYAKADALHKTITDAGYRV+T QNEE+LA+KYRIRLRGIDAPESAMP
Sbjct: 181 NAAIQRSQARNAKNYAKADALHKTITDAGYRVLTVQNEEILARKYRIRLRGIDAPESAMP 240

Query: 241 YGKEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300
           YGKEAKEEL++LVEGKCLRV VYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK
Sbjct: 241 YGKEAKEELRKLVEGKCLRVLVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDK 300

Query: 301 RPELSKWENEARAKRVGLWASSNPEQPWEWRKGKREGK 335
           R EL+KWENEARA+R+GLWAS NPEQPWEWRKGKREGK
Sbjct: 301 RAELAKWENEARARRIGLWASRNPEQPWEWRKGKREGK 337

[Close]
BLAST of CSPI05G11260 vs. TAIR 10
Match: AT2G40410.2 (Staphylococcal nuclease homologue )

HSP 1 Score: 451.4 bits (1160), Expect = 6.2e-127
Identity = 227/333 (68.17%), Postives = 266/333 (79.88%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTS--DSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVV 60
           MGNAL FL G   +PT+  DS  PH  VS++T GVSAL HDLF FEITSQVP GL ++V 
Sbjct: 1   MGNALTFLYGKCCKPTTTDDSLGPH-GVSAATVGVSALAHDLFNFEITSQVPEGLGRYVQ 60

Query: 61  SSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHT 120
           SS+KAQANWY K+ EAWK  KP P+T EEASRLV   LKR+QK DVEGLL+FYGLPL HT
Sbjct: 61  SSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSHT 120

Query: 121 LVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNA 180
           LV+ +   P  VS+P+G+ FE QTLPVD KAVADGDT+TVYVSTS P  SS VP+EV  A
Sbjct: 121 LVEVTVEAP--VSLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNLA 180

Query: 181 ALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYG 240
           A+QR++AR  +NY KAD LH+ I D+GYRV+  +NEEVLA+K+RIRLRGIDAPES MP+G
Sbjct: 181 AVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLRGIDAPESQMPFG 240

Query: 241 KEAKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRP 300
           KEA+E L ++V  K L+V VYGED+Y RCVGDLYCNG F+QEAMLKKG AWHY AYDKRP
Sbjct: 241 KEAQEGLLKIVGRKSLKVLVYGEDQYGRCVGDLYCNGIFVQEAMLKKGLAWHYLAYDKRP 300

Query: 301 ELSKWENEARAKRVGLWASSNPEQPWEWRKGKR 332
            L+KWE EAR KR+GLWASSNPE+PW+WRK  R
Sbjct: 301 VLAKWEKEARQKRIGLWASSNPEKPWDWRKNNR 330

[Close]
BLAST of CSPI05G11260 vs. TAIR 10
Match: AT3G56170.1 (Ca-2+ dependent nuclease )

HSP 1 Score: 447.6 bits (1150), Expect = 8.9e-126
Identity = 227/333 (68.17%), Postives = 261/333 (78.38%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTSDSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVVSS 60
           MGNA+R L     R   +S      VS+S+ GVSAL  DL  FE TSQVP  L  +VVSS
Sbjct: 1   MGNAIRLL-----RKCLNSH----GVSASSGGVSALSRDLLNFETTSQVPEKLGSYVVSS 60

Query: 61  KKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHTLV 120
           +KAQANWY K+ EAWK  KP P+TPEEASRLVI  LK HQK DVEGLL+FYGLP PH LV
Sbjct: 61  QKAQANWYRKILEAWKQAKPRPKTPEEASRLVIAALKNHQKADVEGLLSFYGLPSPHNLV 120

Query: 121 KPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNAAL 180
           +   P    VS+P GV+FEL TLPVD K+VADGDTVTVYVS+  P  SS +PK+V  AA+
Sbjct: 121 E--VPTEAPVSLPKGVRFELNTLPVDTKSVADGDTVTVYVSSKDPLVSSSLPKDVSLAAV 180

Query: 181 QRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLRGIDAPESAMPYGKE 240
           +R++AR  KNY +ADALHKTI  +GYR+I+FQNEEVLAKK+RIRL GID+PES MPYGKE
Sbjct: 181 KRAKAREKKNYTEADALHKTIIASGYRMISFQNEEVLAKKFRIRLSGIDSPESKMPYGKE 240

Query: 241 AKEELKRLVEGKCLRVHVYGEDRYNRCVGDLYCNGKFIQEAMLKKGFAWHYTAYDKRPEL 300
           A +EL ++VEGKCL+V VY EDRY RCVGD+YCNGKF+QE MLKKG AWHY AYDKR EL
Sbjct: 241 AHDELLKMVEGKCLKVLVYTEDRYGRCVGDIYCNGKFVQEVMLKKGLAWHYVAYDKRAEL 300

Query: 301 SKWENEARAKRVGLWASSNPEQPWEWRKGKREG 334
           +KWENEAR KRVGLWASSNPE+PWEWRK KR G
Sbjct: 301 AKWENEARQKRVGLWASSNPEKPWEWRKNKRGG 322

[Close]
BLAST of CSPI05G11260 vs. TAIR 10
Match: AT2G40410.1 (Staphylococcal nuclease homologue )

HSP 1 Score: 280.0 bits (715), Expect = 2.5e-75
Identity = 149/228 (65.35%), Postives = 177/228 (77.63%), Query Frame = 0 

Query: 1   MGNALRFLCGHFSRPTS--DSFPPHAAVSSSTAGVSALPHDLFEFEITSQVPPGLSKHVV 60
           MGNAL FL G   +PT+  DS  PH  VS++T GVSAL HDLF FEITSQVP GL ++V 
Sbjct: 1   MGNALTFLYGKCCKPTTTDDSLGPH-GVSAATVGVSALAHDLFNFEITSQVPEGLGRYVQ 60

Query: 61  SSKKAQANWYAKLAEAWKTEKPTPRTPEEASRLVIQTLKRHQKKDVEGLLTFYGLPLPHT 120
           SS+KAQANWY K+ EAWK  KP P+T EEASRLV   LKR+QK DVEGLL+FYGLPL HT
Sbjct: 61  SSRKAQANWYRKILEAWKQAKPPPQTAEEASRLVTDILKRNQKADVEGLLSFYGLPLSHT 120

Query: 121 LVKPSAPVPTAVSIPDGVKFELQTLPVDAKAVADGDTVTVYVSTSHPRESSCVPKEVRNA 180
           LV+ +   P  VS+P+G+ FE QTLPVD KAVADGDT+TVYVSTS P  SS VP+EV  A
Sbjct: 121 LVEVTVEAP--VSLPEGILFEFQTLPVDPKAVADGDTITVYVSTSEPVVSSSVPREVNLA 180

Query: 181 ALQRSRARNAKNYAKADALHKTITDAGYRVITFQNEEVLAKKYRIRLR 227
           A+QR++AR  +NY KAD LH+ I D+GYRV+  +NEEVLA+K+RIRLR
Sbjct: 181 AVQRAKAREKRNYPKADELHQKIIDSGYRVLNIENEEVLARKFRIRLR 225

The following BLAST results are available for this feature:
BLAST of CSPI05G11260 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q396354.6e-12767.74Uncharacterized 38.1 kDa protein OS=Capnoides sempervirens OX=3464 PE=2 SV=1[more]
F4IH318.7e-12668.17Staphylococcal-like nuclease CAN2 OS=Arabidopsis thaliana OX=3702 GN=CAN2 PE=1 S... [more]
F4IZC51.3e-12468.17Staphylococcal-like nuclease CAN1 OS=Arabidopsis thaliana OX=3702 GN=CAN1 PE=1 S... [more]
Q942N71.0e-11864.48Probable staphylococcal-like nuclease CAN1 OS=Oryza sativa subsp. japonica OX=39... [more]
Q2RBJ23.1e-9152.40Probable staphylococcal-like nuclease CAN2 OS=Oryza sativa subsp. japonica OX=39... [more]
BLAST of CSPI05G11260 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
B6CAZ61.4e-19099.40Calcium-dependent nuclease OS=Cucumis sativus OX=3659 GN=Csa_5G218750 PE=2 SV=1[more]
A0A5D3DQ961.8e-18295.51Staphylococcal-like nuclease CAN1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C4Y11.8e-18295.51staphylococcal-like nuclease CAN1 OS=Cucumis melo OX=3656 GN=LOC103496714 PE=4 S... [more]
A0A6J1FPN52.6e-16585.80staphylococcal-like nuclease CAN2 OS=Cucurbita moschata OX=3662 GN=LOC111447091 ... [more]
A0A6J1K1133.7e-16485.21staphylococcal-like nuclease CAN2 OS=Cucurbita maxima OX=3661 GN=LOC111491421 PE... [more]
BLAST of CSPI05G11260 vs. NCBI nr
Match NameE-valueIdentityDescription
NP_001275526.12.8e-19099.40Uncharacterized 38.1 kDa protein-like [Cucumis sativus] >ABX56707.1 calcium-depe... [more]
XP_008456902.13.7e-18295.51PREDICTED: staphylococcal-like nuclease CAN1 [Cucumis melo] >TYK25847.1 staphylo... [more]
XP_038892991.12.2e-16687.43staphylococcal-like nuclease CAN1 isoform X1 [Benincasa hispida][more]
XP_022941859.15.3e-16585.80staphylococcal-like nuclease CAN2 [Cucurbita moschata] >XP_023552645.1 staphyloc... [more]
XP_022996106.17.7e-16485.21staphylococcal-like nuclease CAN2 [Cucurbita maxima] >XP_022996112.1 staphylococ... [more]
BLAST of CSPI05G11260 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G40410.26.2e-12768.17Staphylococcal nuclease homologue [more]
AT3G56170.18.9e-12668.17Ca-2+ dependent nuclease [more]
AT2G40410.12.5e-7565.35Staphylococcal nuclease homologue [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016071Staphylococcal nuclease (SNase-like), OB-foldSMARTSM00318SNASE_2coord: 199..306
e-value: 1.7E-18
score: 77.5
IPR016071Staphylococcal nuclease (SNase-like), OB-foldPFAMPF00565SNasecoord: 222..301
e-value: 1.0E-21
score: 77.4
IPR016071Staphylococcal nuclease (SNase-like), OB-foldPROSITEPS50830TNASE_3coord: 141..309
score: 24.244762
IPR035437SNase-like, OB-fold superfamilyGENE3D2.40.50.90coord: 138..307
e-value: 7.4E-25
score: 89.6
NoneNo IPR availablePANTHERPTHR12302EBNA2 BINDING PROTEIN P100coord: 12..302
NoneNo IPR availablePANTHERPTHR12302:SF21STAPHYLOCOCCAL-LIKE NUCLEASE CAN2coord: 12..302
NoneNo IPR availableSUPERFAMILY50199Staphylococcal nucleasecoord: 143..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CSPI05G11260.1CSPI05G11260.1mRNA
CSPI05G11260.2CSPI05G11260.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0004518 nuclease activity
molecular_function GO:0003676 nucleic acid binding